CA3088583A1 - Oligonucleotide and nucleic acid synthesis - Google Patents
Oligonucleotide and nucleic acid synthesis Download PDFInfo
- Publication number
- CA3088583A1 CA3088583A1 CA3088583A CA3088583A CA3088583A1 CA 3088583 A1 CA3088583 A1 CA 3088583A1 CA 3088583 A CA3088583 A CA 3088583A CA 3088583 A CA3088583 A CA 3088583A CA 3088583 A1 CA3088583 A1 CA 3088583A1
- Authority
- CA
- Canada
- Prior art keywords
- protecting group
- group
- phosphoramidite
- nucleoside
- sites
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H23/00—Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1801182.5A GB201801182D0 (en) | 2018-01-24 | 2018-01-24 | Oligonucleotide and nucleic acid synthesis |
| GB1801182.5 | 2018-01-24 | ||
| PCT/GB2019/050192 WO2019145713A1 (en) | 2018-01-24 | 2019-01-23 | Oligonucleotide and nucleic acid synthesis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3088583A1 true CA3088583A1 (en) | 2019-08-01 |
Family
ID=61283585
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3088583A Pending CA3088583A1 (en) | 2018-01-24 | 2019-01-23 | Oligonucleotide and nucleic acid synthesis |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US20210047361A1 (https=) |
| EP (1) | EP3743433A1 (https=) |
| JP (2) | JP7493452B2 (https=) |
| KR (1) | KR20200115561A (https=) |
| CN (1) | CN111770928A (https=) |
| AU (1) | AU2019213203B2 (https=) |
| CA (1) | CA3088583A1 (https=) |
| GB (1) | GB201801182D0 (https=) |
| IL (1) | IL276149B (https=) |
| SG (1) | SG11202006741WA (https=) |
| WO (1) | WO2019145713A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11970696B1 (en) | 2019-08-27 | 2024-04-30 | Leidos, Inc. | Optical methods and systems for DNA assembly for computer data storage |
| EP4298246A4 (en) | 2021-02-26 | 2024-12-11 | Avery Digital Data, Inc. | SEMICONDUCTOR CHIP DEVICES AND METHODS FOR SYNTHESIZING POLYNUCLEOTIDES |
| CN113004360A (zh) * | 2021-03-03 | 2021-06-22 | 通用生物系统（安徽）有限公司 | 一种用于膜杂交检测的氨基修饰引物的生产方法 |
| CN114621307B (zh) * | 2022-04-12 | 2024-12-13 | 中国科学院苏州生物医学工程技术研究所 | 一种寡核苷酸空间坐标编码方法及其微流控装置 |
| GB202300626D0 (en) | 2023-01-16 | 2023-03-01 | Evonetix Ltd | Hybridisation detection |
| CN116333006A (zh) * | 2023-04-07 | 2023-06-27 | 苏州欧利生物医药科技有限公司 | 一种带有荧光标记物的寡核苷酸的固相合成方法 |
| US20250206769A1 (en) | 2023-12-22 | 2025-06-26 | Imec Vzw | Synthesizing a Nucleic Acid or Nucleic Acid Analogue |
| WO2025163198A1 (en) | 2024-02-01 | 2025-08-07 | Analog Devices International Unlimited Company | Methods and systems for separating and manipulating a sample comprising a plurality of components |
| WO2025165064A1 (ko) * | 2024-02-02 | 2025-08-07 | 에스티팜 주식회사 | 올리고뉴클레오티드 합성을 위한 개선된 방법 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6270391A (ja) * | 1985-09-25 | 1987-03-31 | Nippon Zeon Co Ltd | 保護されたオリゴヌクレオチドの製造法 |
| US5419874A (en) * | 1992-07-06 | 1995-05-30 | Beckman Instruments, Inc. | Synthesis reaction column |
| US7135565B2 (en) * | 2000-07-28 | 2006-11-14 | Agilent Technologies, Inc. | Synthesis of polynucleotides using combined oxidation/deprotection chemistry |
| WO2004101582A2 (en) * | 2003-05-09 | 2004-11-25 | Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Thermolabile hydroxyl protecting groups and methods of use |
| US8138330B2 (en) * | 2006-09-11 | 2012-03-20 | Sigma-Aldrich Co. Llc | Process for the synthesis of oligonucleotides |
| JP2010248084A (ja) * | 2009-04-10 | 2010-11-04 | Invitrogen Japan Kk | 新規洗浄溶媒を用いるオリゴヌクレオチド合成法 |
-
2018
- 2018-01-24 GB GBGB1801182.5A patent/GB201801182D0/en not_active Ceased
-
2019
- 2019-01-23 CN CN201980015088.5A patent/CN111770928A/zh active Pending
- 2019-01-23 SG SG11202006741WA patent/SG11202006741WA/en unknown
- 2019-01-23 WO PCT/GB2019/050192 patent/WO2019145713A1/en not_active Ceased
- 2019-01-23 JP JP2020539253A patent/JP7493452B2/ja active Active
- 2019-01-23 IL IL276149A patent/IL276149B/en unknown
- 2019-01-23 EP EP19702955.6A patent/EP3743433A1/en active Pending
- 2019-01-23 KR KR1020207024362A patent/KR20200115561A/ko not_active Ceased
- 2019-01-23 AU AU2019213203A patent/AU2019213203B2/en active Active
- 2019-01-23 CA CA3088583A patent/CA3088583A1/en active Pending
- 2019-01-23 US US16/964,390 patent/US20210047361A1/en not_active Abandoned
-
2023
- 2023-07-17 US US18/353,269 patent/US20240199681A1/en active Pending
- 2023-11-24 JP JP2023198864A patent/JP2024023388A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2024023388A (ja) | 2024-02-21 |
| SG11202006741WA (en) | 2020-08-28 |
| US20240199681A1 (en) | 2024-06-20 |
| WO2019145713A9 (en) | 2020-08-06 |
| GB201801182D0 (en) | 2018-03-07 |
| AU2019213203B2 (en) | 2023-02-23 |
| IL276149B (en) | 2022-08-01 |
| AU2019213203A1 (en) | 2020-08-06 |
| US20210047361A1 (en) | 2021-02-18 |
| KR20200115561A (ko) | 2020-10-07 |
| CN111770928A (zh) | 2020-10-13 |
| JP2021510716A (ja) | 2021-04-30 |
| EP3743433A1 (en) | 2020-12-02 |
| AU2019213203A9 (en) | 2020-08-27 |
| JP7493452B2 (ja) | 2024-05-31 |
| WO2019145713A1 (en) | 2019-08-01 |
| IL276149A (en) | 2020-09-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20231229 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 6TH ANNIV.) - STANDARD Year of fee payment: 6 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250121 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT Effective date: 20250121 Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20250121 |
|
| D15 | Examination report completed |
Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT Effective date: 20250206 |
|
| B12 | Application deemed to be withdrawn, abandoned or lapsed |
Free format text: ST27 STATUS EVENT CODE: N-2-6-B10-B12-B303 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO AN EXAMINER'S REQUISITION Effective date: 20250606 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 7TH ANNIV.) - STANDARD Year of fee payment: 7 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20251209 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20251209 |