CN111615552B - 用于颗粒纯化和分馏的微流体芯片 - Google Patents

用于颗粒纯化和分馏的微流体芯片 Download PDF

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CN111615552B
CN111615552B CN201980008710.XA CN201980008710A CN111615552B CN 111615552 B CN111615552 B CN 111615552B CN 201980008710 A CN201980008710 A CN 201980008710A CN 111615552 B CN111615552 B CN 111615552B
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array
column
sample
outlet
fluid
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CN111615552A (zh
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J·史密斯
S·吉福德
金圣哲
B·旺施
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International Business Machines Corp
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1894—Cooling means; Cryo cooling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
CN201980008710.XA 2018-01-19 2019-01-14 用于颗粒纯化和分馏的微流体芯片 Active CN111615552B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15/875,905 2018-01-19
US15/875,905 US10946380B2 (en) 2018-01-19 2018-01-19 Microfluidic chips for particle purification and fractionation
PCT/IB2019/050272 WO2019142087A1 (en) 2018-01-19 2019-01-14 Microfluidic chips for particle purification and fractionation

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CN111615552A CN111615552A (zh) 2020-09-01
CN111615552B true CN111615552B (zh) 2023-07-11

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US (2) US10946380B2 (enExample)
JP (1) JP2021510516A (enExample)
CN (1) CN111615552B (enExample)
DE (1) DE112019000463B4 (enExample)
GB (1) GB2583625A (enExample)
WO (1) WO2019142087A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11458474B2 (en) 2018-01-19 2022-10-04 International Business Machines Corporation Microfluidic chips with one or more vias
US20190226953A1 (en) 2018-01-19 2019-07-25 International Business Machines Corporation Microscale and mesoscale condenser devices
US10946380B2 (en) 2018-01-19 2021-03-16 International Business Machines Corporation Microfluidic chips for particle purification and fractionation
US20200316230A1 (en) * 2019-04-08 2020-10-08 Vanderbilt University Devices and methods for radiopharmaceutical synthesis
US11266989B2 (en) * 2019-08-07 2022-03-08 International Business Machines Corporation Immunodetection and separation on nanoDLD
US11517873B2 (en) 2019-10-29 2022-12-06 International Business Machines Corporation Controlled chemical synthesis using polymer substrates and nanofluidic separation systems
US11565262B2 (en) * 2020-03-10 2023-01-31 International Business Machines Corporation Continous band-pass filter size separation using a negative angle DLD array
CN111821601A (zh) * 2020-05-27 2020-10-27 深圳艾素科技有限公司 一种电子过滤装置及其操作方法
CN111778138B (zh) * 2020-07-06 2022-01-11 中南大学 一种分选血浆中外泌体的微流控器件及其使用方法
CN113652333B (zh) * 2021-08-03 2023-03-21 中国科学院上海微系统与信息技术研究所 一种优化流体分布的微柱式多相位移通道
CN114574357B (zh) * 2022-02-14 2025-11-11 大连交通大学 一种基于确定性侧向位移和介电泳技术联用的微藻与细菌分选装置
TWI889038B (zh) * 2023-04-14 2025-07-01 前瞻優勢生醫股份有限公司 微流體微粒分選器
CN117504956B (zh) * 2023-10-16 2024-12-17 北京大学 一种微纳流控芯片及生化标志物分子的分离方法
WO2025212802A1 (en) * 2024-04-02 2025-10-09 The General Hospital Corporation Molecular analysis of cytology specimens

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105050718A (zh) * 2013-03-06 2015-11-11 哈佛学院院长及董事 形成相对单分散液滴的装置和方法
CN107110765A (zh) * 2014-11-03 2017-08-29 通用医疗公司 在微流体装置中浓缩颗粒

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2726018B2 (ja) 1995-07-07 1998-03-11 株式会社サクション瓦斯機関製作所 多管式熱交換器
US7892681B2 (en) 2005-07-19 2011-02-22 Pelton Walter E System of distributed electrochemical cells integrated with microelectronic structures
ES2341103T3 (es) 2002-10-23 2010-06-15 The Trustees Of Princeton University Procedimiento de separacion continua de particulas usando reticulados de obstaculos alineados asimetricamente respecto a campos.
WO2005100401A2 (en) * 2004-03-31 2005-10-27 Adnagen Ag Monoclonal antibodies with specificity for fetal erythroid cells
US7281387B2 (en) 2004-04-29 2007-10-16 Carrier Commercial Refrigeration Inc. Foul-resistant condenser using microchannel tubing
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
JP2008538282A (ja) * 2005-04-05 2008-10-23 セルポイント ダイアグノスティクス， インコーポレイテッド 装置および循環腫瘍細胞および他の粒子の濃縮および変更のための方法
WO2006124597A2 (en) 2005-05-12 2006-11-23 Foster Ron B Infinitely stackable interconnect device and method
US7863189B2 (en) 2007-01-05 2011-01-04 International Business Machines Corporation Methods for fabricating silicon carriers with conductive through-vias with low stress and low defect density
FR2931141B1 (fr) 2008-05-13 2011-07-01 Commissariat Energie Atomique Systeme microfluidique et procede pour le tri d'amas de cellules et de preference pour leur encapsulation en continu suite a leur tri
EP2304414B1 (en) 2008-07-24 2017-03-15 The Trustees of Princeton University Bump array device having asymmetric gaps for segregation of particles
US8093099B2 (en) 2008-09-26 2012-01-10 International Business Machines Corporation Lock and key through-via method for wafer level 3D integration and structures produced
WO2010124155A1 (en) 2009-04-23 2010-10-28 Logos Energy, Inc. Lateral displacement array for microfiltration
US9039996B2 (en) 2010-10-12 2015-05-26 Stmicroelectronics, Inc. Silicon substrate optimization for microarray technology
CN102052803B (zh) 2010-12-13 2012-11-28 上海环球制冷设备有限公司 集成冷凝满液式蒸发器装置及使用方法
WO2012094642A2 (en) 2011-01-06 2012-07-12 On-Q-ity Circulating tumor cell capture on a microfluidic chip incorporating both affinity and size
US20170271207A9 (en) 2011-01-29 2017-09-21 International Business Machines Corporation Novel 3D Integration Method Using SOI Substrates And Structures Produced Thereby
CN102856329B (zh) 2011-06-30 2015-02-11 中芯国际集成电路制造(上海)有限公司 一种硅通孔封装方法
CN105247042B (zh) * 2013-03-15 2021-06-11 普林斯顿大学理事会 用于高通量纯化的方法和设备
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
CN110186835B (zh) 2013-03-15 2022-05-31 Gpb科学有限公司 颗粒的片上微流体处理
WO2014166000A1 (en) * 2013-04-11 2014-10-16 The Governing Council Of The University Of Toronto Device for capture of particles in a flow
US10202577B2 (en) 2013-10-18 2019-02-12 The General Hospital Corporation Microfluidic sorting using high gradient magnetic fields
JP6604945B2 (ja) 2013-11-22 2019-11-13 ザ ジェネラル ホスピタル コーポレイション 粒子クラスタを単離するためのマイクロ流体方法およびシステム
US9636675B2 (en) 2014-11-26 2017-05-02 International Business Machines Corporation Pillar array structure with uniform and high aspect ratio nanometer gaps
US9835538B2 (en) 2014-11-26 2017-12-05 International Business Machines Corporation Biopolymer separation using nanostructured arrays
US10058895B2 (en) 2014-11-26 2018-08-28 International Business Machines Corporation Continuous flow, size-based separation of entities down to the nanometer scale using nanopillar arrays
CN105161473A (zh) 2015-07-09 2015-12-16 江苏中圣压力容器装备制造有限公司 硅基毛细泵回路微型冷却器
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
CN105293428B (zh) 2015-10-19 2017-04-19 北京航天控制仪器研究所 一种mems器件的全硅化圆片级真空封装方法及封装器件
US9700891B2 (en) 2015-11-13 2017-07-11 International Business Machines Corporation Integrated nanofluidic arrays for high capacity colloid separation
WO2017087940A1 (en) 2015-11-20 2017-05-26 University Of Kansas Non-invasive monitoring cancer using integrated microfluidic profiling of circulating microvesicles
US10507466B2 (en) 2016-04-27 2019-12-17 International Business Machines Corporation Metal assisted chemical etching for fabricating high aspect ratio and straight silicon nanopillar arrays for sorting applications
CN109328098A (zh) 2016-06-20 2019-02-12 凸版印刷株式会社 液体介质的置换方法及用于该方法的流路设备
US10386276B2 (en) 2016-09-20 2019-08-20 International Business Machines Corporation Phosphoprotein detection using a chip-based pillar array
US10010883B2 (en) * 2016-09-20 2018-07-03 International Business Machines Corporation Deterministic lateral displacement arrays
US10253350B2 (en) 2016-09-20 2019-04-09 International Business Machines Corporation Separation of molecules using nanopillar arrays
US20200038861A1 (en) * 2016-10-07 2020-02-06 The Regents Of The University Of Michigan Systems and methods for high throughput screening
US20190226953A1 (en) 2018-01-19 2019-07-25 International Business Machines Corporation Microscale and mesoscale condenser devices
US10946380B2 (en) 2018-01-19 2021-03-16 International Business Machines Corporation Microfluidic chips for particle purification and fractionation
US11149298B2 (en) 2018-06-13 2021-10-19 International Business Machines Corporation Detection of nucleic acid sequences using deterministic lateral displacement arrays
US11033901B2 (en) 2018-10-23 2021-06-15 International Business Machines Corporation Biomarker detection using integrated purification-detection devices

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105050718A (zh) * 2013-03-06 2015-11-11 哈佛学院院长及董事 形成相对单分散液滴的装置和方法
CN107110765A (zh) * 2014-11-03 2017-08-29 通用医疗公司 在微流体装置中浓缩颗粒

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US20190224677A1 (en) 2019-07-25
US11872560B2 (en) 2024-01-16
US20210114028A1 (en) 2021-04-22
DE112019000463B4 (de) 2024-04-25
JP2021510516A (ja) 2021-04-30
GB202010486D0 (en) 2020-08-19
DE112019000463T5 (de) 2020-10-01
US10946380B2 (en) 2021-03-16
WO2019142087A1 (en) 2019-07-25
CN111615552A (zh) 2020-09-01
GB2583625A (en) 2020-11-04

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