JP2021510516A - 粒子の精製および分画のための方法およびマイクロ流体装置 - Google Patents
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Abstract
Description
Claims (20)
- マイクロ流体チップの層を含む装置であって、前記層は入口と、出口と、前記入口および前記出口と流体連絡する凝縮器アレイとを含み、前記凝縮器アレイは1.0ナノリットル毎時以上のスループット速度を有する、装置。
- 前記層の入口は流体を受け取り、前記出口は前記流体の精製バージョンを送出し、前記凝縮器アレイは複数の列に配置された複数の支柱を含み、前記複数の列の第1の列内の前記複数の支柱の第1の支柱と、前記第1の列内の前記複数の支柱の第2の支柱との間に、1.0ナノリットル毎時以上の前記流体のスループット速度を促進するようにサイズ決めされた支柱間隙が位置する、請求項1に記載の装置。
- 前記マイクロ流体チップは、生物学的サンプル流体を精製するために設計されたラボ・オン・チップである、請求項2に記載の装置。
- 前記装置は、ハンドヘルド・デバイスまたは精製システムの少なくとも1つを含む、請求項2に記載の装置。
- 前記層は、ナノスケール決定論的横置換アレイをさらに含み、前記ナノスケール決定論的横置換アレイは前記凝縮器アレイに結合され、前記ナノスケール決定論的横置換アレイは前記凝縮器アレイにおいて精製される前記流体の粒子を分離する、請求項2に記載の装置。
- 前記装置はさらに、前記入口と結合されて流体連絡する第1のリザーバと、前記出口と結合されて流体連絡する第2のリザーバとを含み、前記第1のリザーバは前記凝縮器アレイによって精製される前記流体を受け取り、前記第2のリザーバは前記出口から送出される前記流体の前記精製バージョンを受け取る、請求項2に記載の装置。
- 前記複数の支柱は、前記凝縮器アレイを通って流れる流体を収集壁に向かって横方向に移動させる格子を定める、請求項2に記載の装置。
- 前記入口は入口バスと流体連絡し、前記入口バスは前記収集チャネルと流体連絡し、前記収集チャネルは出口バスと流体連絡し、前記出口バスは前記出口と流体連絡する、請求項7に記載の装置。
- 前記複数の支柱は格子を定め、第1の比率は第1の定められた値以下であり、前記第1の比率はDx/Dyを特徴とし、ここでDxは前記格子を第1の方向に横切る第1の距離を表し、Dyは前記格子を第2の方向に横切る第2の距離を表し、前記第1の方向は前記第2の方向と直交する、請求項2に記載の装置。
- 第2の比率は第2の定められた値よりも大きく、前記第2の比率はD0/Dyを特徴とし、ここでD0は前記複数の支柱の直径を表す、請求項9に記載の装置。
- 凝縮器アレイと、入口と、出口とを含むマイクロ流体チップにおいて緩衝溶液およびサンプル溶液を受け取るステップであって、前記サンプル溶液はサンプルおよび不要物を含み、前記緩衝溶液および前記サンプル溶液は、約1.0ナノリットル毎時よりも大きい速度で前記凝縮器アレイを通って流れる、ステップと、
前記凝縮器アレイによって、前記サンプル溶液からの前記サンプルを前記マイクロ流体チップの側壁に対して横の方向に移動させるステップであって、前記サンプルは前記緩衝溶液内に移動する、ステップと、
前記出口を介して前記不要物から分離した前記サンプルを収集するステップとを含む、方法。 - 前記凝縮器アレイは、マイクロスケール凝縮器アレイおよびメソスケール凝縮器アレイからなる群より選択される、請求項11に記載の方法。
- 前記凝縮器アレイは格子を定める複数の支柱を含み、第1の比率は第1の定められた値以下であり、前記第1の比率はDx/Dyを特徴とし、ここでDxは前記格子を第1の方向に横切る第1の距離を表し、Dyは前記格子を第2の方向に横切る第2の距離を表し、前記第1の方向は前記第2の方向と直交し、第2の比率は第2の定められた値よりも大きく、前記第2の比率はD0/Dyを特徴とし、ここでD0は前記複数の支柱の直径を表す、請求項12に記載の方法。
- 前記第1の定められた値は1.0であり、前記第2の定められた値は0.5である、請求項13に記載の方法。
- 前記マイクロ流体チップに前記緩衝溶液および前記サンプル溶液を前記受け取るステップは圧力駆動される、請求項12に記載の方法。
- ナノスケール決定論的横置換アレイを介して前記サンプルを分画するステップをさらに含む、請求項15に記載の方法。
- 前記凝縮器アレイは複数の列に配置された複数の支柱を含み、前記複数の列の第1の列内の前記複数の支柱の第1の支柱と、前記第1の列内の前記複数の支柱の第2の支柱との間に約0.5マイクロメートル以上の支柱間隙が位置しており、前記第1の支柱は前記第2の支柱と隣り合っている、請求項1に記載の装置。
- 前記複数の支柱は格子を定め、第1の比率は第1の定められた値以下であり、前記第1の比率はDx/Dyを特徴とし、ここでDxは前記格子を第1の方向に横切る第1の距離を表し、Dyは前記格子を第2の方向に横切る第2の距離を表し、前記第1の方向は前記第2の方向と直交し、第2の比率は第2の定められた値よりも大きく、前記第2の比率はD0/Dyを特徴とし、ここでD0は前記複数の支柱の直径を表す、請求項1に記載の装置。
- 前記第1の定められた値は1.0であり、前記第2の定められた値は0.5である、請求項1に記載の装置。
- 前記層は、ナノスケール決定論的横置換アレイをさらに含む、請求項1に記載の装置。
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US20190226953A1 (en) | 2018-01-19 | 2019-07-25 | International Business Machines Corporation | Microscale and mesoscale condenser devices |
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US10946380B2 (en) | 2018-01-19 | 2021-03-16 | International Business Machines Corporation | Microfluidic chips for particle purification and fractionation |
US11266989B2 (en) * | 2019-08-07 | 2022-03-08 | International Business Machines Corporation | Immunodetection and separation on nanoDLD |
US11517873B2 (en) | 2019-10-29 | 2022-12-06 | International Business Machines Corporation | Controlled chemical synthesis using polymer substrates and nanofluidic separation systems |
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