CN111567485A - Method for feeding white muscardine silkworms in small scale - Google Patents
Method for feeding white muscardine silkworms in small scale Download PDFInfo
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- CN111567485A CN111567485A CN202010568663.9A CN202010568663A CN111567485A CN 111567485 A CN111567485 A CN 111567485A CN 202010568663 A CN202010568663 A CN 202010568663A CN 111567485 A CN111567485 A CN 111567485A
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- silkworms
- silkworm
- spore suspension
- feeding
- beauveria bassiana
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 96
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000000725 suspension Substances 0.000 claims abstract description 26
- 241000751139 Beauveria bassiana Species 0.000 claims abstract description 21
- 238000005728 strengthening Methods 0.000 claims abstract description 14
- 241000255791 Bombyx Species 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 9
- 239000002689 soil Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000008213 purified water Substances 0.000 claims description 10
- 230000007958 sleep Effects 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 239000004576 sand Substances 0.000 claims description 7
- 239000005995 Aluminium silicate Substances 0.000 claims description 6
- 235000012211 aluminium silicate Nutrition 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 6
- 235000008708 Morus alba Nutrition 0.000 claims description 4
- 240000000249 Morus alba Species 0.000 claims description 4
- 238000005360 mashing Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 238000005482 strain hardening Methods 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims 5
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 10
- 238000011081 inoculation Methods 0.000 abstract description 6
- 241000233866 Fungi Species 0.000 abstract description 5
- 208000015181 infectious disease Diseases 0.000 abstract description 5
- 230000002458 infectious effect Effects 0.000 abstract description 5
- 230000001954 sterilising effect Effects 0.000 abstract description 5
- 238000009987 spinning Methods 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 208000009132 Catalepsy Diseases 0.000 description 2
- 206010047853 Waxy flexibility Diseases 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
- 238000009317 small scale farming Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicinal Chemistry (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a method for feeding white muscardine silkworms in a small scale, which comprises the following steps: s1, feeding the silkworms waiting for the application of the fungus to be rigidified; s2, performing enlarged strengthening cultivation on the beauveria bassiana strain; s3, preparing spore suspension from fresh Bombyx Batryticatus, and applying the spore suspension. According to the method, the fresh stiff silkworm and the kwan-yin soil are adopted to prepare the spore suspension, the inoculation success rate is high, the infectious microbes are not easy to infect, a small amount of commercial beauveria bassiana spore powder is adopted to perform enlarged strengthening cultivation, and the cost can be reduced; if the silkworm larva is not died regularly or is not died after the expanded strengthening cultivation inoculation, the disinfection and sterilization treatment can be carried out in time, the disinfection and sterilization are very easy to be completed due to small amount, other silkworms which are not inoculated with the beauveria bassiana can be inoculated with other strains or can be normally raised until spinning and cocooning, the cultivation risk is low, and the cultivation income of the traditional silkworm growers can be kept stable.
Description
Technical Field
The invention relates to the technical field of silkworm breeding, in particular to a method for breeding white muscardine silkworms in a small scale.
Background
Silkworm, also called bombyx mori, is a lepidoptera seriator, belongs to invertebrate, the armoda moth family of bombyx mori silkworm species, the silkworm moth larvae take mulberry leaves as foodstuff, the silkworm passes through 4 morphological and physiological completely different development stages of eggs, larvae, pupae and imagoes in the whole life, and different parts and metabolites thereof in each development stage have wide medicinal value. Silkworm moth larvae spit out a large amount of silk for cocooning before pupation, which is an important textile raw material, and at present, silkworm moth larvae are infected with beauveria bassiana to harvest the white muscardine silkworm used as a traditional Chinese medicinal material, so that the current society has great demands on silkworm cocoons, silk and white muscardine silkworms.
The traditional silkworm farmer needs to cultivate white muscardine silkworm on a small scale, the silkworm in the previous season is generally adopted, spore liquid is obtained by washing, silkworm bodies are sprayed before the five-year-old silkworm falls asleep and takes a sound to eat, the silkworm in the previous season is stored for a long time, the preservation environment of the general farmer is poor, the silkworm is provided with a plurality of mixed bacteria, the spore liquid is also provided with a plurality of mixed bacteria, the silkworm bodies are easy to infect the mixed bacteria in the process of spraying the spore liquid, and the strain, the dosage of the spore liquid and the like cannot be mastered due to the fact that the general silkworm farmer does not have professional detection instruments such as a microscope and the like, so that the stiff rate is low or the stiff is early, the quality of the silkworm. The beauveria bassiana needs to be stored at low temperature, so that the spore activity of commercial strains can not be ensured in the links of transportation, storage and the like, the commercial strains are more difficult to master by common silkworm farmers, and the commercial strains are high in price and large in dosage and are not accepted by the silkworm farmers.
Disclosure of Invention
The present invention is directed to a method for feeding Bombyx Batryticatus in a small scale, which solves the problems of the background art mentioned above.
The technical scheme provided by the invention is as follows:
a method for feeding Bombyx Batryticatus on a small scale comprises the following steps:
s1, feeding the silkworms waiting for the strain hardening: after normal silkworms are raised to the age of three to sleep, properly selecting the silkworms raised in the batch to a raising box or an independent room for later use;
s2, expanding and strengthening the white muscardine strains: selecting commercial beauveria bassiana spore powder, adding kwan-yin soil according to the use specification to prepare spore suspension, spraying the prepared spore suspension liquid to silkworm bodies by using an agricultural sprayer or a small portable spray can, feeding mulberry leaves after half a small time for normal feeding, and waiting for complete rigidification for later use;
s3, preparing spore suspension with fresh Bombyx Batryticatus and applying: mashing the fresh muscardine silkworm in the previous step S2 into mud, adding purified water and kaolin to prepare spore suspension, filtering with a sand screen, and spraying with a sprayer to wet the silkworm.
Preferably, in step S1, 1% of the silkworms in the group are selected to be kept in a breeding box or an independent room for future use.
Preferably, the purified water is added in step S3 to prepare spore suspension by using 1000g of purified water and 10g of kaolin for 20000 silkworms in the early stage of five-year-old dormancy.
Preferably, in step S3, three-layer overlapping filtering is performed with 100-mesh sand net.
The invention has the beneficial effects that:
1. the expanded and strengthened silkworm accounts for a few, the culture environment is easy to sterilize, the infectious microbes on the fresh stiff silkworms are few, the spore activity of the beauveria bassiana in the fresh stiff silkworms is optimal, the fresh stiff silkworms and the kwan-yin soil are locally adopted to prepare the spore suspension for inoculating the silkworms, the inoculation success rate is high, the silkworms are not easily infected with the infectious microbes, and the problem that the environment is polluted by strain diffusion possibly occurring in the storage and transportation processes is avoided.
2. According to the method, a small amount of commercial beauveria bassiana spore powder is adopted for enlarged strengthening culture, so that the cost can be reduced, the infectivity of commercial strains can be observed, if the commercial strains are not died neatly or are not died after enlarged strengthening culture inoculation, disinfection and sterilization can be timely carried out, the amount is small, disinfection and sterilization are extremely easy to complete, other silkworms which are not inoculated with beauveria bassiana can be inoculated with other strains or normally fed to spinning and cocooning, the culture risk is small, the method is beneficial to keeping stable culture income of traditional silkworm farmers, is more suitable for small-scale silkworm culture of farmers, is beneficial to popularization of silkworm culture in broad farmers, and has positive significance for development of the silkworm culture industry.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and the embodiments described below are only a part of the embodiments of the present invention, but not all of the embodiments.
This example provides a method for feeding Bombyx Batryticatus on a small scale, comprising the steps of:
s1, feeding the silkworms waiting for the strain hardening: after normal silkworms are raised to the age of three to sleep, properly selecting the silkworms raised in the batch to a raising box or an independent room for later use;
s2, expanding and strengthening the white muscardine strains: selecting commercial beauveria bassiana spore powder, adding kwan-yin soil according to the use specification to prepare spore suspension, spraying the prepared spore suspension liquid to silkworm bodies by using an agricultural sprayer or a small portable spray can, feeding mulberry leaves after half a small time for normal feeding, and waiting for complete rigidification for later use; it should be noted that, in the step, the silkworm catalepsy can occur within about five days, when all the silkworms for the beauveria bassiana strain expanded strengthening cultivation are completely catalepsy, other normally cultivated silkworms are just in the early stage of five-year-old onset of sleep, the early stage of five-year-old onset of sleep is a window period for inoculating the beauveria bassiana to the silkworms, and the success rate of inoculating the beauveria bassiana to the silkworms is extremely high;
s3, preparing spore suspension with fresh Bombyx Batryticatus and applying: mashing the fresh muscardine silkworm in the previous step S2 into mud, adding purified water and kaolin to prepare spore suspension, filtering with a sand screen, and spraying with a sprayer to wet the silkworm.
The ratio of expanded and strengthened silkworms is very small, the breeding environment is easy to sterilize, the infectious microbes on the fresh silkworms are very few, the spore activity of the beauveria bassiana in the fresh silkworms is optimal, the silkworms are inoculated by using the spore suspension prepared from the fresh silkworms and the kwan-yin soil, the kwa-yin soil and the water can be uniformly suspended and distributed in the water after being mixed, the spores of the beauveria bassiana and the kwa-yin soil are adhered together and can be uniformly suspended and distributed in the water, the spores of the beauveria bassiana in the spore suspension are uniformly distributed, and the kwa-yin soil also has a certain adhesion effect, the sprayed spore suspension can be well adhered to the surface of a silkworm body, the inoculation success rate is high, and the silkworms are not easy to infect the infectious microbes; through the step S2, the white muscardine fungi required by the small-scale farming of farmers can be obtained only by adopting a small amount of commercial white muscardine fungi spore powder for enlarged strengthening cultivation, the production cost of the farmers can be effectively reduced, the white muscardine fungi spore powder is more suitable for the small-scale farming of the farmers, the promotion of the silkworm farming in broad farmers is facilitated, and the white muscardine fungi spore powder has positive significance for the development of the silkworm farming industry.
In addition, the method adopts a small amount of commercial beauveria bassiana spore powder to carry out expanded strengthening culture, can reduce the cost, and can observe the infectivity of commercial strains, for example, after the expanded strengthening culture is inoculated, the strains die irregularly or do not become rigid, and can be disinfected and killed in time, the quantity is small, the disinfection and the sterilization are easy to complete, other silkworms which are not inoculated with the beauveria bassiana can be inoculated by other strains or normally fed to spinning and cocooning, the culture risk is small, and the method is favorable for keeping the culture income of the traditional silkworm raisers stable.
In a preferred embodiment of the present invention, in the step S1, 1% of the silkworms in the batch are selected to be kept in a cultivation box or an independent room for further use. Through actual production practice, 1% of the silkworms after the three-instar initial sleep period bred in the batch are selected for subsequently expanding, strengthening and breeding the beauveria bassiana, and the spore suspension prepared from the obtained fresh stiff silkworms is enough for inoculating other silkworms in the five-instar initial sleep period of the batch.
In a preferred embodiment of the present invention, the purified water is added in the step S3 to prepare spore suspension by adding 1000g of purified water and 10g of kaolin to 20000 silkworms in the early stage of five-year-old sleep. Through actual production practice, when the silkworm subjected to the expanded strengthening cultivation of the beauveria bassiana strain is completely rigidified, other silkworms are just in the early stage of five-year-old sleep, 20000 silkworms are suitable for one silkworm, and the obtained fresh stiff silkworms are prepared into a spore suspension according to 1000g of purified water of one silkworm, so that the final inoculation effect is good.
In a preferred embodiment of the present invention, the step S3 is performed by three-layer overlapping filtering with a 100-mesh sand screen. According to the invention, the spore suspension prepared from the fresh batryticated silkworm is filtered by the three-layer overlapped 100-mesh sand screen, so that the residues in the spore suspension can be removed, and the spraying equipment cannot be blocked in the spraying process.
The above-described embodiments are merely preferred embodiments of the present invention, which are intended to be illustrative and not limiting, and any modifications, equivalents, improvements and the like which are not inventive changes may be made within the spirit of the present invention and the scope of the claims without departing from the scope of the present invention.
Claims (4)
1. A method for feeding Bombyx Batryticatus on a small scale, which is characterized by comprising the following steps:
s1, feeding the silkworms waiting for the strain hardening: after normal silkworms are raised to the age of three to sleep, properly selecting the silkworms raised in the batch to a raising box or an independent room for later use;
s2, expanding and strengthening the white muscardine strains: selecting commercial beauveria bassiana spore powder, adding kwan-yin soil according to the use specification to prepare spore suspension, spraying the prepared spore suspension liquid to silkworm bodies by using an agricultural sprayer or a small portable spray can, feeding mulberry leaves after half a small time for normal feeding, and waiting for complete rigidification for later use;
s3, preparing spore suspension with fresh Bombyx Batryticatus and applying: mashing the fresh muscardine silkworm in the previous step S2 into mud, adding purified water and kaolin to prepare spore suspension, filtering with a sand screen, and spraying with a sprayer to wet the silkworm.
2. The method for rearing a silkworm on a small scale according to claim 1, wherein 1% of silkworms in the silkworm rearing lot are selected in step S1 and kept in a rearing box or a separate room for later use.
3. The method for rearing white silkworm according to claim 1, wherein the amount of purified water added in the step S3 is such that a spore suspension is prepared from 1000g of purified water and 10g of kaolin to 20000 silkworms in the early stage of five instars of sleep.
4. The method for rearing a Bombyx Batryticatus on a small scale according to claim 1, wherein the three-layer overlapping filtration is carried out with a 100 mesh sand screen in the step S3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010568663.9A CN111567485A (en) | 2020-06-19 | 2020-06-19 | Method for feeding white muscardine silkworms in small scale |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010568663.9A CN111567485A (en) | 2020-06-19 | 2020-06-19 | Method for feeding white muscardine silkworms in small scale |
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| Publication Number | Publication Date |
|---|---|
| CN111567485A true CN111567485A (en) | 2020-08-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010568663.9A Pending CN111567485A (en) | 2020-06-19 | 2020-06-19 | Method for feeding white muscardine silkworms in small scale |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5255979A (en) * | 1975-10-30 | 1977-05-07 | Mikasa Kagaku Kogyo Kk | Method of preventing silkworm from muscardine |
| CN103923841A (en) * | 2014-04-14 | 2014-07-16 | 广东省农业科学院蚕业与农产品加工研究所 | Beauveria bassiana strain having high pathogenicity to silkworms and application thereof |
| CN104642393A (en) * | 2015-01-29 | 2015-05-27 | 江西省新龙生物科技有限公司 | Insect virus and beauveria bassiana mixing insecticide suspending agent and preparation method thereof |
| CN105770657A (en) * | 2016-05-09 | 2016-07-20 | 薛俊芳 | Traditional Chinese medicinal preparation for treating enterobiasis and preparation method thereof |
| CN106719472A (en) * | 2016-12-01 | 2017-05-31 | 四川省农业科学院蚕业研究所 | A kind of production method of the larva of a silkworm with batrytis |
-
2020
- 2020-06-19 CN CN202010568663.9A patent/CN111567485A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5255979A (en) * | 1975-10-30 | 1977-05-07 | Mikasa Kagaku Kogyo Kk | Method of preventing silkworm from muscardine |
| CN103923841A (en) * | 2014-04-14 | 2014-07-16 | 广东省农业科学院蚕业与农产品加工研究所 | Beauveria bassiana strain having high pathogenicity to silkworms and application thereof |
| CN104642393A (en) * | 2015-01-29 | 2015-05-27 | 江西省新龙生物科技有限公司 | Insect virus and beauveria bassiana mixing insecticide suspending agent and preparation method thereof |
| CN105770657A (en) * | 2016-05-09 | 2016-07-20 | 薛俊芳 | Traditional Chinese medicinal preparation for treating enterobiasis and preparation method thereof |
| CN106719472A (en) * | 2016-12-01 | 2017-05-31 | 四川省农业科学院蚕业研究所 | A kind of production method of the larva of a silkworm with batrytis |
Non-Patent Citations (2)
| Title |
|---|
| 曹恒生: "《药用真菌的栽培与临床》", 31 May 1986 * |
| 赵根楠等: "《中国菇类栽培手册》", 31 January 1990 * |
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