CN1561715A - Aseptic cultivating method for newly-hatched silkworm - Google Patents
Aseptic cultivating method for newly-hatched silkworm Download PDFInfo
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- CN1561715A CN1561715A CN 200410017109 CN200410017109A CN1561715A CN 1561715 A CN1561715 A CN 1561715A CN 200410017109 CN200410017109 CN 200410017109 CN 200410017109 A CN200410017109 A CN 200410017109A CN 1561715 A CN1561715 A CN 1561715A
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Abstract
An aseptic culture method for silkworm larvae includes disinfecting silkworm ova one day before they ar incubated by immersing in formaldehyde solution for 10-15 min and then in alcohol for 1-4 min, drying in air, inculating to obtain larvae and culturing.
Description
Technical field
The present invention relates to the breeding method of the aseptic newly-hatched silkworm of silkworm.
Background technology
Along with the developmental research that silkworm produces multiple biological product as bio-reactor, the demand of silkworm is increasing, and traditional sericulture not only is subjected to season and region restriction, and plants mulberry and also strengthen day by day with the contradiction that kind grain and other economic crops strive ground, struggle for the work force between farming and nonfarming sectors.Artificial diet rearing can provide silkworm whenever and wherever possible, alleviates to plant mulberry and strive the contradiction of ground, struggle for the work force between farming and nonfarming sectors with planting grain and other economic crops, raises labour productivity greatly and reduces labour intensity.The silkworm artificial feed is because nutritious, and the moisture height is added the higher temperatures humidity requirement of raising environment, very easily causes the feed moldy metamorphism, and causes the weak and flacherie generation of silkworm body.Common silkworm seed is owing to implement to hasten the hatching of silkworms, and unavoidable chorion carries disease germs, in case newly-hatched silkworm gnaws chorion when hatching, the silkworm body is carried disease germs.So the cultivation of aseptic newly-hatched silkworm is to guarantee one of key technology of artificial diet rearing silkworm.
Summary of the invention
It is simple, easy to operate, time saving and energy saving to the purpose of this invention is to provide a kind of method, helps the breeding method of the aseptic newly-hatched silkworm of applying in the rural area.
The breeding method of the aseptic newly-hatched silkworm of silkworm of the present invention, it is characterized in that the egg for silk production body pigmentation stage that hastens the hatching of silkworms, is that 4% formaldehyde flooded 10-15 minute in newly-hatched silkworm hatching proxima luce (prox. luc) to silkworm seed concentration, be 95% disinfection in alcohol dipping 1-4 minute again with concentration after pulling out, place aseptic place to dry naturally then, continue to mend and hasten the hatching of silkworms, until egg-incubation, obtain aseptic newly-hatched silkworm, the raising of collecting ants.
Find the feed mildew from collecting ants to through more than 6 days, young silkworm grew to 2 later stages in age, was suitable for carrying out therefore that educate for 1 age for 1 time or educate for 2 ages for 1 time.
The inventive method is simple, easy to operate, time saving and energy saving, helps the rural area and applies.
Embodiment
Embodiment 1
With the egg for silk production body pigmentation stage that hastens the hatching of silkworms, hatch two groups of last bus in newly-hatched silkworm, respectively to the disinfection of two groups of silkworm seed faces, wherein, first group is 4% formaldehyde dipping 15 minutes with concentration, is 95% alcohol dipping 1 minute with concentration again after pulling out.Second group is 4% formaldehyde dipping 10 minutes with concentration, is 95% alcohol dipping 4 minutes with concentration again after pulling out.To through two groups of silkworm seeds of disinfecting after dry naturally in aseptic place, continue to mend according to a conventional method to hasten the hatching of silkworms, until egg-incubation, obtain aseptic newly-hatched silkworm, the raising of collecting ants.
With above-mentioned first, second this two groups handle the thin mao of rate of control group newly-hatched silkworm and grow through the aseptic newly-hatched silkworm of disinfecting and non-sterile and compare, the results are shown in Table 1.
Can find out from table 1, see that the thin hair rate in two groups of aseptic newly-hatched silkworm districts is a little more than the check plot with ingesting property of feed, the sterilization back to 1 age silkworm growth have no adverse effects.
The thin hair rate of the different disinfection of egg surface silkworms of table 1 and growing
| The disinfection of egg surface | Dredge hair rate % | 1 age is through (h) | 1 hibernating worm body weight (g/ head) |
| Control group | ????79.7 | ????101 | ????0.005 |
| First group | ????80.0 | ????101 | ????0.006 |
| Second group | ????83.0 | ????101 | ????0.006 |
Embodiment 2
Embodiment 1 described first, second two groups of incubation rates with control group are compared, the results are shown in Table 2, as can be seen from Table 2, the disinfection of egg surface is handled does not almost have influence to incubation rate.
The incubation rate of the different disinfection of egg surfaces of table 2
| Incubation rate on the one | Incubation rate on the two | |
| Control group | ????95.9 | ????/ |
| First group | ????85.4 | ????95.6 |
| Second group | ????87.6 | ????95.3 |
Embodiment 3
Described two groups of the aseptic newly-hatched silkworms of embodiment 1 through disinfection of egg surface hatching, the situation of going mouldy of its artificial feed is investigated in raisings of collecting ants, and with control group (being placed with the test region of ovum, chorion and newly-hatched silkworm that non-sterile crosses) comparison, the results are shown in Table 3.Find that aseptic newly-hatched silkworm has postponed 42h than the feed of control group and just gone mouldy, ovum, chorion and newly-hatched silkworm of this explanation non-sterile have direct influence to going mouldy of artificial feed.
Relation between different ovum sterilizations of table 3 and artificial feed go mouldy
| The ovum sterilization method | The 1st time (h:min) that mildew occurs | Feed mouldy 50% through time (h:min) | ||
| The 1st batch of test | The 2nd batch of test | The 1st batch of test | The 2nd batch of test | |
| Control group | ????51:40 | ????55:00 | ????118:00 | ????143:00 |
| First group | ????174:20 | ????168:00 | ????197:20 | ????190:00 |
| Second group | ????150:20 | ????156:20 | ????197:20 | ????174:00 |
Can obviously find out from table 3, change blue or green ovum all can postpone artificial feed after the disinfection of egg surface mouldy time, find mildew from collecting ants to through more than 6 days, young silkworm grew to 2 middle and later periods in age, therefore had ready conditions fully and may carry out for 1 age and educate for 1 time.
Claims (1)
1. the breeding method of aseptic newly-hatched silkworm, it is characterized in that the egg for silk production body pigmentation stage that hastens the hatching of silkworms, is that 4% formaldehyde flooded 10-15 minute in newly-hatched silkworm hatching proxima luce (prox. luc) to silkworm seed concentration, be 95% disinfection in alcohol dipping 1-4 minute again with concentration after pulling out, place aseptic place to dry naturally then, continue to mend and hasten the hatching of silkworms, until egg-incubation, obtain aseptic newly-hatched silkworm, the raising of collecting ants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410017109 CN1561715A (en) | 2004-03-19 | 2004-03-19 | Aseptic cultivating method for newly-hatched silkworm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410017109 CN1561715A (en) | 2004-03-19 | 2004-03-19 | Aseptic cultivating method for newly-hatched silkworm |
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| Publication Number | Publication Date |
|---|---|
| CN1561715A true CN1561715A (en) | 2005-01-12 |
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| CN 200410017109 Pending CN1561715A (en) | 2004-03-19 | 2004-03-19 | Aseptic cultivating method for newly-hatched silkworm |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100334947C (en) * | 2005-07-14 | 2007-09-05 | 苏州大学 | Hatching control of silkworm eggs |
| CN101911927A (en) * | 2010-09-08 | 2010-12-15 | 江苏民星茧丝绸股份有限公司 | Incubation method for enhancing day hatchability rate of silkworm eggs |
| CN104542508A (en) * | 2014-12-31 | 2015-04-29 | 重庆市黔江区福子保种植股份合作社 | Silkworm breeding method |
| CN106804618A (en) * | 2016-11-30 | 2017-06-09 | 河池市技术开发中心 | A kind of disinfectant for cultivating young silkworm containing PVP I |
| CN106804616A (en) * | 2016-11-30 | 2017-06-09 | 河池市技术开发中心 | A kind of disinfectant for cultivating young silkworm containing Peracetic acid |
| CN109566543A (en) * | 2018-12-28 | 2019-04-05 | 中国农业科学院植物保护研究所 | A method of improving Candle-sticks stinkbug egg laying amount |
| CN112273339A (en) * | 2020-10-30 | 2021-01-29 | 来宾市农业科学院 | Simple ant collecting method for artificial feed breeding of silkworms |
-
2004
- 2004-03-19 CN CN 200410017109 patent/CN1561715A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100334947C (en) * | 2005-07-14 | 2007-09-05 | 苏州大学 | Hatching control of silkworm eggs |
| CN101911927A (en) * | 2010-09-08 | 2010-12-15 | 江苏民星茧丝绸股份有限公司 | Incubation method for enhancing day hatchability rate of silkworm eggs |
| CN101911927B (en) * | 2010-09-08 | 2011-09-28 | 江苏民星茧丝绸股份有限公司 | Incubation method for enhancing day hatchability rate of silkworm eggs |
| CN104542508A (en) * | 2014-12-31 | 2015-04-29 | 重庆市黔江区福子保种植股份合作社 | Silkworm breeding method |
| CN106804618A (en) * | 2016-11-30 | 2017-06-09 | 河池市技术开发中心 | A kind of disinfectant for cultivating young silkworm containing PVP I |
| CN106804616A (en) * | 2016-11-30 | 2017-06-09 | 河池市技术开发中心 | A kind of disinfectant for cultivating young silkworm containing Peracetic acid |
| CN109566543A (en) * | 2018-12-28 | 2019-04-05 | 中国农业科学院植物保护研究所 | A method of improving Candle-sticks stinkbug egg laying amount |
| CN112273339A (en) * | 2020-10-30 | 2021-01-29 | 来宾市农业科学院 | Simple ant collecting method for artificial feed breeding of silkworms |
| CN112273339B (en) * | 2020-10-30 | 2022-03-25 | 来宾市农业科学院 | Simple ant collecting method for artificial feed breeding of silkworms |
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