CN111534579B - 基于捕获测序的大片段重排检测的捕获探针、试剂盒和检测方法 - Google Patents
基于捕获测序的大片段重排检测的捕获探针、试剂盒和检测方法 Download PDFInfo
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Abstract
本发明公开了一种基于捕获测序的大片段重排(LGR)检测方法。本发明的捕获探针包括外显子探针和单核苷酸多态性位点(SNP)探针。外显子探针包括针对基因每一个外显子及外显子两端60bp区域的至少3个探针，能够对外显子及两端60bp区域2X全覆盖。SNP探针包括针对基因内含子区域以及基因的上下游各5000bp范围内人群发生频率介于30％‑70％的SNP位点。同时，本发明还提供一种由序列深度算法模块、等位基因不平衡算法模块和非一致序列算法模块整合而成的算法，用于对NGS测序数据进行大片段重排的分析。本发明能够有效检测LGR，降低对核酸样本的纯度要求、减少单碱基变异引起的假阳性，对外显子扩增也具有较强的检测性能。
Description
技术领域
本发明涉及一种基于捕获测序的大片段重排检测的捕获探针、试剂盒和检测方法。
背景技术
大片段重排(large genomic rearrangement,LGR)，是指基因组中出现的大片段DNA断裂重组现象，会导致重排区域基因的功能变化，包括外显子的缺失(deletion)和扩增(duplication)。LGR是一类重要的致病性突变，其准确检测对于临床实践至关重要。
目前在临床实践中，多重连接依赖探针扩增技术(multiple ligation-dependentprobe amplification,MLPA)是应用最广泛的检测BRCA1/2大片段基因组重排的方法。MLPA有三个主要弊端：1.对于核酸样本的纯度要求较高：酒精，金属离子，苯酚和Trizol都会影响MLPA的检测性能；2.当探针结合位点DNA序列发生单碱基变异(SNV)时，容易产生假阳性。因此临床上检测到单外显子缺失(Exon deletion)，需要进一步确认是否是由SNV引起的假阳性；3.MLPA对duplication的检测性能较差：duplication的信号水平相较deletion低，在采样数据点少且高噪音的背景下难以判定是否为真实duplication变异。
除了MLPA技术，现有技术中的大片段重排检测方法还有多重扩增子定量(Multiplex Amplicon Quantification，MAQ)和扩增子测序(Amplicon-Seq)，都是基于多重扩增技术的方法。这类方法受限于扩增子(amplicon)的数量，每个外显子只能设计1到2个扩增子，使得每个外显子的数据采样点只有1个或2个。而样本历经采集，运输，核酸抽提，实验反应等环节，各环节在不同条件下累积的噪音水平不同，使得实验数据的噪音水平差别很大，导致一些样本，不能满足大片段重排检测的要求。另外，当探针或者引物区域出现SNV时，会由于扩增效率的降低导致假阳性，特别是对单外显子缺失或扩增的检测性能不够。
BRCA1与BRCA2是重要的抑癌基因，参与同源重组过程中的DNA损伤修复。BRCA1/2大片段重排，是一类重要的BRCA1/2致病性突变，包括外显子缺失(Exon deletion)和外显子扩增(Exon duplication)，约占总突变数目的10％。现已发现超过120种BRCA1和40种BRCA2基因大片段重排。检测BRCA突变对于提示遗传风险和指导临床用药有重要作用。
因此本领域为解决传统检测方法的弊端，急需新的基因大片段重排检测技术。
发明内容
本发明涉及一种基于捕获测序的大片段重排检测的捕获探针、试剂盒和检测方法。
本发明提供一种基于下一代测序技术检测大片段重排的捕获探针及检测方法：1.探针设计覆盖每个外显子和外显子两端60bp并设计至少3个探针，增加序列深度(Readsdepth)算法模块的数据点来提高Reads depth算法模块结果的准确性；2.针对杂合单核苷酸多态性(SNP)位点设计探针捕获，用于等位基因不平衡(Allelic imbalance)算法模块(此算法根据杂合SNP的等位基因频率判断是否发生了LGR变异)，排除假阳性；3.检测算法上，整合了Reads depth模块,Allelic imbalance模块和Discordant reads算法模块，相互验证，优势互补，提高检测性能。
本发明第一方面，提供了一组适用于大片段重排检测的捕获探针，包括针对基因每一个外显子及所述外显子两端60bp区域的探针，和针对单核苷酸多态性(SNP)位点的探针。
进一步的，所述捕获探针能够对每一个外显子及外显子两端60bp区域2X全覆盖，所述外显子及其两端60bp的区域至少有3条不同探针覆盖。
进一步的，所述SNP位点为基因内含子区域以及基因的上下游各5000bp范围内的SNP位点。
进一步的，所述SNP位点的人群发生频率(参考1000genomes和GenomeAggregation Database人群频率数据库)介于30％-70％。
进一步的，所述基因为BRCA1/2。
进一步的，所述捕获探针为SEQ ID NO:1～584所示的探针的混合物。
本发明第二方面，提供了一种基于下一代测序平台检测大片段重排的试剂盒，包括本发明第一方面所述的捕获探针。
本发明第三方面，提供了本发明第一方面所述捕获探针在制备按照如下方法检测大片段重排的试剂盒中的应用，所述方法包括以下步骤：
(1)对目标样本进行DNA抽提；
(2)制备测序文库；
(3)测序，并进行生信流程分析。
进一步的，所述步骤(2)包括以下步骤：
(a)将DNA片段化，然后再对片段的末端进行修复、磷酸化，之后在片段的3’端加脱氧腺嘌呤(A)，再连接接头；
(b)进行扩增反应，扩增后的产物与上述的捕获探针进行杂交，杂交后对片段进行特异性洗脱，再进行PCR扩增，得到测序文库。
本发明第四方面，提供了一种适用于本发明第三方面所述应用中生信流程分析的算法，其特征在于，所述算法是由序列深度(Reads depth)算法模块、等位基因不平衡(Allelic imbalance)算法模块和非一致序列(Discordant reads)算法模块整合而成的。
进一步的，先综合序列深度算法模块和非一致序列算法模块对结果进行处理，确认潜在的大片段重排。
进一步的，对潜在的大片段重排应用等位基因不平衡算法模块处理，以排除假阳性，确定真实的大片段重排。
本发明在探针设计上，覆盖每个外显子和外显子两端60bp，并在每个ROI(regionof interest)区域(外显子和外显子两端60bp)设计至少3个探针，增加Reads depth算法模块的采样点，提高了结果的准确性；整合了外显子区域、内含子区域及基因上下游区域内杂合SNP位点覆盖，用于Allelic imbalance算法模块排除假阳性。
检测算法上，整合了Reads depth、Allelic imbalance和Discordant reads算法模块，相互验证，优势互补，提高检测性能，特别是提高了对单外显子缺失或单外显子扩增的检测能力。
本发明适应各种基因LGR变异(如BRCA1/2大片段重排)的检测，降低对核酸样本的纯度要求，灵敏度高，减少单碱基变异引起的假阳性，对外显子扩增也具有较强的检测性能。
附图说明
图1.分析流程图
图2.Allelic imbalance算法模块排除假阳性逻辑图
图3.A样本BRCA1基因exon17-exon22扩增
图4.B样本BRCA1基因exon1-exon8杂合缺失
图5.C样本BRCA2基因exon20融合
图6.D样本BRCA1基因exon21假阳性缺失
图7.E样本BRCA2基因exon9假阳性扩增
具体实施方式
现详细说明本发明的多种示例性实施方式，该详细说明不应认为是对本发明的限制，而应理解为是对本发明的某些方面、特性和实施方案的更详细的描述。
应理解本发明中所述的术语仅仅是为描述特别的实施方式，并非用于限制本发明。另外，对于本发明中的数值范围，应理解为具体公开了该范围的上限和下限以及它们之间的每个中间值。在任何陈述值或陈述范围内的中间值以及任何其他陈述值或在所述范围内的中间值之间的每个较小的范围也包括在本发明内。这些较小范围的上限和下限可独立地包括或排除在范围内。
除非另有说明，否则本文使用的所有技术和科学术语具有本发明所述领域的常规技术人员通常理解的相同含义。虽然本发明仅描述了优选的方法和材料，但是在本发明的实施或测试中也可以使用与本文所述相似或等同的任何方法和材料。
本发明提供一种基于下一代测序技术检测大片段重排的捕获探针及检测方法：1.探针设计覆盖每个外显子和外显子两端60bp并设计至少3个探针，增加序列深度(Readsdepth)算法模块的数据点来提高Reads depth算法模块结果的准确性；2.针对杂合单核苷酸多态性(SNP)位点设计探针捕获，用于等位基因不平衡(Allelic imbalance)算法模块(此算法根据杂合SNP的等位基因频率判断是否发生了LGR变异)，排除假阳性；3.检测算法上，整合了Reads depth模块，Allelic imbalance模块和Discordant reads算法模块，相互验证，优势互补提高了检测性能。应理解，本发明的捕获探针及检测方法(包括算法模块的组合)可普遍适应于不同基因或基因组合的大片段重排的检测，而不仅限于某种特定的基因，更不限于实施例中的示例基因。
捕获探针的设计
探针指的是长度通常在20至300个碱基长度的DNA、RNA及其他核酸衍生物(包括但不限于LNA等)的核酸小分子。这类通常会与一些功能基团(包括但不限于生物素biotin等其他基团)连接。探针会与目标DNA片段以碱基互补的形式结合，而探针上的功能基团会和与之有强亲和性的其他功能基团(包括但不限于链霉亲和素Streptavidin、卵白素avidin等)或特异性抗体相结合。通常与探针上功能基团相结合的其他功能基团或抗体被连接到磁珠、有吸附性的材料等耗材上，并通过物理的手段将目标片段和探针的结合体从反应溶液中提取出来，达到捕获目标片段的目的。
本发明针对大片段重排(LGR)变异检测的特点对探针进行个性化设计。
本发明的创新点在于特异性探针的设计方案：1.针对所有外显子及外显子两端60bp区域的探针，为增加数据点，提高算法的准确度，探针对外显子及外显子两端60bp区域2X全覆盖，并保证每个外显子至少3个不同探针覆盖。2.针对性地在基因内含子区域以及基因的上下游各5000bp范围内单核苷酸多态性(SNP)位点设计探针，包括但不限于选择人群发生频率(参考1000genomes和Genome Aggregation Database人群频率数据库)介于30％～70％的单核苷酸多态性(SNP)位点进行探针设计将其捕获。应理解，除了内含子以及基因的上下游各5000bp范围内的SNP位点，本发明的探针还覆盖所有外显子区域的SNP位点。所有SNP位点的测序信息用于后续算法模块的分析。
应理解，本发明的捕获探针设计方法可普遍适应于不同基因或基因组合的大片段重排的检测，而不仅限于某种特定的基因，更不限于实施例中的示例基因，实施例仅用于示例性说明本发明，并不限定本发明的适用范围。具体地，针对BRCA1/2基因探针如SEQ IDNO:1～584所示，具体探针序列见序列表。
样本选择
本发明的样本类型包括但不限于以下样本类型：血液白细胞、血浆游离DNA、新鲜组织、石蜡切片等。本发明的检测范围不局限于胚系变异，也包括体细胞变异。
捕获测序步骤
在取得组织或者血液样本后，首先利用市场上成熟的试剂盒(如：QIAGEN56404或QIAGEN51306)对DNA进行抽提。对于抽提的DNA，需要利用超声技术(例如：Covaris:PN500295超声打断仪)、DNA酶切技术(例如：KAPA：KK8602试剂盒)或者转座酶技术(例如：HieffNGS:12206ES08试剂盒)进行打断，形成200个碱基长度左右的双链DNA片段。然后针对来源不同的DNA利用对应的试剂盒(如：IDT-2003200或KAPA-KK8504)进行处理和文库的构建。建库完成后，按照试剂盒(如：IDT-2003200或KAPA-KK8504)使用说明将一个至四个样本的文库进行混合，然后用专门设计合成的BRCA1/2大片段重排捕获探针进行片段捕获。捕获探针可以与其他探针混合后一起进行捕获，探针的捕获效率不会受到影响。捕获好的文库最终通过高通量测序仪(如Illumina NovaSeq 6000system)进行测序。
测序数据处理和分析
DNA样本在测序后会得到原始的测序结果，其结果为大量短片段序列。对这些短片段序列进行以下几个模块的处理和分析。分析流程如图1所示。
详细步骤如下：
i.数据预处理和质控模块：
1.对原始测序数据，用fastp v0.20.0软件过滤掉低质量的测序序列，并进行数据质量评估。
2.使用bwa v0.7.12软件将过滤后的高质量数据比对(mapping)到人类基因组参考序列(GRCh37)上。
3.使用picard v1.8.0_221软件去除PCR duplication reads。
ii.序列深度(Reads depth)算法模块
1.根据比对结果中reads的位置使用本地python脚本计算出每个探针区域内reads的数量(reads count)。
2.使用背景样本(未发生LGR变异的样本)库(panel of normal，PON)的readscount为baseline，对样本的reads count进行标准化，再做对数运算(以2为底的对数运算：log2)转换为logR值。
3.使用本地Python脚本计算探针区间内的GC含量(GC content)。
4.使用R v3.6.0软件和局部加权回归模型(Lowess)对GC含量构建回归模型并进行预测，进而对logR进行降噪。
5.将降噪后的logR值和阈值(cutoff)进行比较，判断是否为缺失(deletion)、扩增(duplication)或正常(normal)。
iii.等位基因不平衡(Allelic imbalance)算法模块：
1.提取探针区域内所有杂合SNP位点(包括外显子区域、内含子区域、以及基因上下游各5000bp范围内的杂合SNP位点)的等位基因支持reads数(allelic reads)。
2.使用文献(Degner JF,et al.Effect of read-mapping biases on detecting
allele-specific expression from RNA-sequencing data.Bioinformatics.
2009,25(24):3207–12.)中的方法对allelic reads数据进行校正。
3.使用矫正后的allelic reads数据计算SNP位点的突变基因型频率(B allelefrequency，BAF)。
4.对BAF值做如下变换：
5.根据计算得到的MAF(mirror allele frequency)，使用R V3.6.0中的DNAcopy模块做区块划分(segmentation)。
6.针对于每个区块(segment)计算MAF的标准差(MAF.sd)，作为等位基因平衡(allelic balance)的基线(baseline)。
7.计算所有区块MAF.sd的均值(sd.mean)与标准差(sd.sd)，并设定Allelicimbalance的阈值(cutoff)为：sd.mean+sd.sd×3。
针对每个区块计算BAF的标准差(BAF.sd)，并与上述cutoff进行比较，判断是否为balance或imbalance：当BAF.sd大于cutoff时判定为imbalance，当BAF.sd小于cutoff时判定为balance。
iv.非一致序列(Discordant reads)算法模块：
1.根据比对文件中reads的比对信息提取BRCA1/2基因及其上下游各5000bp范围内的discordant reads。
2.根据discordant reads的软剪切(soft clip)的位置推断出融合变异(fusion)的断点(breakpoint)。
3.根据断点推导出融合序列，并以断点两端1000bp序列建立人工染色体(artifact chromosome)。
4.将融合区域内所有的reads重新比对到artifact chromosome上。
5.根据融合序列的比对结果计算出跨越序列(junction reads)和断裂序列(split reads)的数目。
6.根据预设的junction reads和split reads的阈值(根据大量无LGR变异样本计算junction reads和split reads上限数值为5，将其设置为阈值)判断是否为融合变异(fusion)或正常(normal)。
v.综合算法模块：
根据Reads depth算法模块和Discordant reads算法模块的结果搜寻潜在的LGR变异。再根据Allelic imbalance算法模块的判定结果排除假阳性的LGR变异。三模块关系与综合判定步骤如图1虚线内所示。
1.搜寻步骤：结合Reads depth算法模块的结果和Discordant reads算法模块的结果，给出潜在的LGR变异区域。
2.排除步骤：如图2所示，以Allelic imbalance算法模块判定结果为依据，判断潜在LGR是否真实：
a)当潜在LGR为deletion时按照如下情况判断：
i.对应区域内没有观测到杂合SNP位点，则Allelic imbalance算法模块判定与deletion不矛盾，保留此LGR变异。
ii.对应区域内观测到杂合SNP位点，则Allelic imbalance算法模块不支持此deletion，舍弃此LGR变异。
b)当潜在LGR为duplication时按照如下情况判断：
i.对应区域内没有观测到杂合SNP位点，则Allelic imbalance算法模块无法进一步判断其真实性，保留此LGR变异。
ii.对应区域内观测到杂合SNP位点：如果Allelic imbalance算法模块判别为imbalance且BAF值与Reads depth算法模块计算得出的logR值相符，则保留此LGR变异；否则Allelic imbalance算法模块不支持此duplication，舍弃此LGR变异。
实施例
实施例1组织样本的制备和测序
使用QIAamp DNA FFPE Tissue Kit(50)(产品号：56404)提取组织样本的DNA，然后使用超声波破碎仪Covaris Focused-ultrasonicator(产品号：S220)将提取的DNA打断为200bp左右的片段，然后再对片段的末端进行修复、磷酸化，之后在片段的3’端加脱氧腺嘌呤(A)，再连接接头。使用ProFlex 3x 32well PCR system对处理后片段进行扩增，扩增后的产物与探针(SEQ ID NO:1～584)进行杂交，杂交后对片段进行特异性洗脱，再进行PCR扩增，然后对扩增后的产物进行定量和片段长度分布测定。合格后使用Illumina的NovaSeq6000System进行测序，测序深度不低于500X。
实施例2血液样本的制备和测序
使用QIAamp DNA Mini Kit(250)(产品号：51306)提取外周血白细胞中的核DNA，和组织样本一样使用超声波破碎仪Covaris Focused-ultrasonicator(产品号：S220)将提取的DNA打断为200bp左右的片段，然后再对片段的末端进行修复、磷酸化，之后在片段的3’端加脱氧腺嘌呤(A)，再连接接头。使用ProFlex 3x 32well PCR system对处理后片段进行扩增，扩增后的产物与探针(SEQ ID NO:1～584)进行杂交，杂交后对片段进行特异性洗脱，再进行PCR扩增，然后对扩增后的产物进行定量和片段长度分布测定。合格后使用Illumina的NovaSeq 6000System进行测序，测序深度不低于500X。
实施例3生物信息学分析
1测序数据预处理与质控
使用bcl2fastq v2.19.0软件将测序下机文件(bcl格式)转换为序列文件(fastq格式)，然后使用fastp v0.20.0软件对序列文件进行质控(QC)和过滤(filter)，去除低质量序列，再使用bwa v0.7.12软件将高质量的干净序列比对到人类参考基因组(GRCh37)上，生成比对文件(bam格式)，使用picardv1.8.0_221对比对文件进行排序和去重(PCRduplication)处理，使用bcftoolsv0.1.19软件包检测出目标区域内所有位点的基因型和对应深度信息(depth)，使用本地Python脚本计算探针区域的GC含量。
2潜在LGR变异的初步判定
(1)Reads depth判定：统计每条探针区间的序列数目(reads count)，然后计算出背景样本的reads count的baseline，使用baseline进行标准化，再对数化(log)得到探针的logR值：
由于探针的logR值受到GC含量等因素影响，所以使用局部加权回归模型(Lowess)以及背景样本数据拟合出回归模型model，其中logR为背景样本的logR值，GC为探针的GC含量值：
model＝lowess(logR～GC)
使用模型对GC含量影响进行预测，其中sample.GC为检测样本的GC含量值，检测样本为实施例1的组织样本或实施例2的血液样本：
GC.pred＝predict(model,sample.GC)
使用预测结果GC.pred对logR进行降噪处理，其中sample.logR为检测样本的logR值：
sample.logR.correct＝sample.logR-GC.pred
使用R v3.6.0软件中的DNAcopy模块对降噪后的sample,logR.correct值进行区块划分(segmentation)，并计算每一个区块(segment)标准化的segment.logR。根据阈值(计算得出大量无LGR变异样本的logR值上下限分别为0.5和-0.7，将其设置为阈值)判定每一个segment是否为LGR变异：
当segment.logR大于等于0.5时判定为扩增(duplication)并根据segment.logR计算出扩增倍数(copy number)；当segment.logR小于-0.7时判定为缺失(deletion)；其余情况判定为normal。
copy number＝2×(2segment，logR)
(2)Allelic imbalance判定：根据数据预处理模块生成的位点基因型和深度信息去除深度低于20X的位点，然后计算位点的等位基因型频率(AF)，其中ref.depth为野生基因型深度，alt.depth为突变基因型深度：
AF＝ref.depth/alt.depth
使用所有SNP位点AF值的中位数(AF.median)对ref.depth和alt.depth进行矫正：
根据矫正之后的ref.depth.correct和alt.depth.correct计算突变基因型频率(BAF)：
BAF＝alt.depth.correct/(ref.depth.correct+alt.depth.correct)
选择BAF值介于0.05～0.95之间的位点进行后面的分析。
根据Reads depth模块划分的区块对每个区块计算BAF值整体的标准差(BAF.sd)，然后对区块内的BAF值做如下变换：
根据Reads depth模块划分的区块计算每个区块MAF的标准差(MAF.sd)，作为allelic balance的baseline。计算所有区块MAF.sd的均值(sd.mean)与标准差(sd.sd)，并设定allelic imbalance的阈值(cutoff)为sd.mean+sd.sd×3。
针对每个区块计算BAF的标准差(BAF.sd)，并与上述cutoff进行比较，判断是否为balance或imbalance：当BAF.sd大于cutoff时判定为imbalance，当BAF.sd小于cutoff时判定为balance。
(3)Discordant reads判定：从预处理模块生成的比对结果(bam)文件中提取包含断点的序列(split reads)和两个断点附近成对的序列(junction reads)，根据splitreads的软剪切信息使用本地Python脚本计算出潜在融合的断点位置(breakpoint)，根据breakpoint位置生成融合基因组序列，以断点两端1000bp序列建立人工染色体(artifactchromosome)。将区域内所有的reads比对到artifact chromosome上，生成新的比对结果，统计junction reads和split reads的数目：当junction reads在潜在融合变异两端的比对长度不低于25bp时，junction reads计数加一；当split reads在断点两侧的长度均大于20bp时split reads数目加一。最后根据junction reads和split reads数目判定是否为融合变异(fusion)：当潜在融合变异同时具有junction reads和split reads且数目均大于等于5时则判定为fusion；否则判定为normal。
3潜在LGR变异综合判定
(1)Reads depth算法模块与Discordant reads算法模块结果合并，搜寻潜在LGR变异：基因内部发生融合(融合的上下游基因为同一个基因)，既表现为融合(fusion)也表现为缺失(deletion)；而不同基因之间发生融合变异时，既可能表现为deletion也可能表现为duplication。当融合断点位于探针捕获区域内部时表现为split reads和junctionreads等融合特性且断点位置精确。故当Discordant reads结果为fusion时根据Readsdepth计算得出的logR值判断是否为deletion或duplication：当logR小于等于0.5时判定为deletion；否则判定为duplication。
(2)根据Allelic imbalance结果对(1)的合并结果进行确定性判定和假阳性判定：
当(1)的结果为deletion或fusion时，如果Allelic imbalance结果为imbalance或目标区域没有杂合SNP位点则认为Allelic imbalance支持deletion或fusion变异，即此变异为真实变异，如果Allelic imbalance结果为balance则认为Allelic imbalance不支持deletion或fusion变异，即此变异为假阳性结果。
当(1)的结果为duplication时，如果Allelic imbalance结果为imbalance且目标区域内BAF服从均值为(copy number-1)/(copy number)的正态分布则认为Allelicimbalance支持duplication变异，即此变异为真实变异，否则认为Allelic imbalance不支持duplication变异，即此变异为假阳性结果。
判定结果如表1所示：
表1.各样本判定结果
*其中Allelic imbalance列中的none表示潜在LGR变异区域内为杂合SNP；dup即duplication；del即deletion；“-”表示“normal”。
如图3所示，A样本BRCA1基因exon17-exon22扩增(duplication)：Reads depth模块判断exon17-exon22区域出现duplication变异，Allelic imbalance模块判定此区域为imbalance且BAF值与Reads depth模块计算得出的copy number相符，故判定此duplication为真。
如图4所示，B样本BRCA1基因exon1-exon8杂合缺失(deletion)：Reads depth模块判断exon1-exon8区域出现deletion变异，Allelic imbalance模块判定此区域为imbalance且区域内的SNP位点均为纯合类型，与杂合缺失变异相符，故判定此deletion为真。
如图5所示，C样本BRCA2基因exon20融合(fusion)：Discordant reads模块显示exon20两侧出现大量split reads和junction reads，判定此区域发生fusion变异。Readsdepth模块计算得出对应区域的logR值并未超出阈值而判定为deletion或duplication，但logR值显著小于0.5，因此判断此潜在LGR变异为deletion类型。由于潜在LGR变异区域内未发现杂合SNP位点，Allelic imbalance模块无法做出判断。故判定此deletion为真。
如图6所示，D样本BRCA1基因exon21假阳性缺失(false deletion):Reads depth模块结果显示exon21出现潜在deletion变异，但Allelic imbalance模块判定此区域为balance，存在可靠的杂合SNP，并且Discordant reads模块判定为normal，未发现splitreads和junction reads，故判定此deletion为假。
如图7所示，E样本BRCA2基因exon9假阳性扩增(false duplication):Readsdepth模块结果显示exon9出现潜在duplication变异，但Allelic imbalance模块判定此区域为balance，且Discordant reads模块判定为normal，故判定此duplication为假。
实施例4结果验证
将发明的检测方法和作为LGR检测金标准的MLPA技术同时对38例样本进行了检测，结果如下表2所示，通过比较得到本发明的灵敏度、特异性均为100％。表3是依照表2的统计。
表2交叉验证样本信息表
| 样本编号 | MLPA检测结果 | 本发明检测结果 |
| 7115 | BRCA2：exon17-18Del | BRCA2：exon17-18Del |
| 9071 | BRCA1：exon1-2Del | BRCA1：exon1-2Del |
| 9309 | BRCA1：exon1-2Del | BRCA1：exon1-2Del |
| 13194 | normal | normal |
| 13227 | normal | normal |
| 13645 | BRCA1：exon3De 1 | BRCA1：exon3Del |
| 14303 | BRCA1：exon1 7-22Dup | BRCA1：exon17-22Dup |
| 14493 | BRCA1：exon14Del | BRCA1：exon14Del |
| 14504 | BRCA1：exon3Del | BRCA1：exon3Del |
| 14849 | BRCA1：exon7Dup | BRCA1：exon7Dup |
| 14960 | normal | normal |
| 16223 | BRCA1：exon9-11Dup | BRCA1：exon9-11Dup |
| 18485 | BRCA1：exon1-2Dup | BRCA1：exon1-2Dup |
| 25925 | BRCA2：exon19-20Dup | BRCA2：exon19-20Dup |
| 26395 | BRCA1：exon19-23Dup | BRCA1：exon19-23Dup |
| 27665 | BRCAl：exon1-2Dup | BRCA1：exon1-2Dup |
| 27666 | BRCA1：exon17-18Del | BRCA1：exon17-18Del |
| 31968 | normal | normal |
| 33846 | BRCA1：exon22Del | BRCA1：exon22Del |
| 34704 | BRCA1：exon7Del | BRCA1：exon7Del |
| 34878 | BRCA1：exon4-6Del | BRCA1：exon4-6Del |
| 35285 | BRCA1：exon1-9Del | BRCA1：exon1-9Del |
| 37225 | normal | normal |
| 37604 | BRCA1：exon1-2Del | BRCA1：exon1-2De l |
| 41574 | BRCA2：exon17-18Del | BRCA2：exon17-18Del |
| 43192 | BRCA2：exon2-17Del | BRCA2：exon2-17Del |
| 43816 | BRCA1：exon7Del | BRCA1：exon7Del |
| 45044 | BRCA1：exon7Del | BRCA1：exon7Del |
| 46073 | BRCA1：exon8Del | BRCA1：exon8Del |
| 47341 | BRCA1：exon7Del | BRCA1：exon7Del |
| 48711 | BRCA2：exon20De l | BRCA2：exon20Del |
| 54730 | BRCA1：exon7Del | BRCA1：exon7Del |
| 54773 | BRCA1：exon2Del | BRCA1：exon2Del |
| 55313 | BRCA1：exon7-8Del | BRCA1：exon7-8Del |
| 55708 | BRCA2：exon22-24Del | BRCA2：exon22-24Del |
| 55869 | BRCA1：exon22Del | BRCA1：exon22Del |
| 57825 | BRCA2：exon22-24Del | BRCA2：exon22-24Del |
| 57889 | BRCA2：exon14-18Del | BRCA2：exon14-18Del |
表3本发明与MLPA检测结果对比
真阳性样本数(true positive，TP)：MLPA与本发明检测均为阳性的样本数；假阳性样本数(false positive，FP)：MLPA检测为阴性但本发明检测为阳性的样本数；假阴性样本数(false negative，FN)：MLPA检测为阳性但本发明检测为阴性的样本数；真阴性样本数(true negative，TN)：MLPA与本发明检测均为阴性样本数。灵敏度＝TP/(TP+FN)；特异性＝TN/(TN+FP)。
序列表
<110> 上海思路迪医学检验所有限公司
<120> 基于捕获测序的大片段重排检测的捕获探针、试剂盒和检测方法
<130> LT1912044F
<160> 584
<170> SIPOSequenceListing 1.0
<210> 1
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
cggcgtcacg tggccagcgc gggcttgtgg cgcgagcttc tgaaactagg cggcagaggc 60
ggagccgctg tggcactgct gcgcctctgc tgcgcctcgg gtgtcttttg cggcggtggg 120
<210> 2
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
ggagccgctg tggcactgct gcgcctctgc tgcgcctcgg gtgtcttttg cggcggtggg 60
tcgccgccgg gagaagcgtg aggggacaga tttgtgaccg gcgcggtttt tgtcagctta 120
<210> 3
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
tcgccgccgg gagaagcgtg aggggacaga tttgtgaccg gcgcggtttt tgtcagctta 60
ctccggccaa aaaagaactg cacctctgga gcgggttagt ggtggtggta gtgggttggg 120
<210> 4
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
aaggaatgca tccctgtgta agtgcatttt ggtcttctgt tttgcagact tatttaccaa 60
gcattggagg aatatcgtag gtaaaaatgc ctattggatc caaagagagg ccaacatttt 120
<210> 5
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
gcattggagg aatatcgtag gtaaaaatgc ctattggatc caaagagagg ccaacatttt 60
ttgaaatttt taagacacgc tgcaacaaag caggtattga caaattttat ataactttat 120
<210> 6
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
ttgaaatttt taagacacgc tgcaacaaag caggtattga caaattttat ataactttat 60
aaattacacc gagaaagtgt tttctaaaaa atgcttgcta aaaacccagt acgtcacagt 120
<210> 7
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
tttttcctta tgatctttaa ctgttctggg tcacaaattt gtctgtcact ggttaaaact 60
aaggtgggat ttttttttta aatagattta ggaccaataa gtcttaattg gtttgaagaa 120
<210> 8
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
aaggtgggat ttttttttta aatagattta ggaccaataa gtcttaattg gtttgaagaa 60
ctttcttcag aagctccacc ctataattct gaacctgcag aagaatctga acataaaaac 120
<210> 9
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
ctttcttcag aagctccacc ctataattct gaacctgcag aagaatctga acataaaaac 60
aacaattacg aaccaaacct atttaaaact ccacaaagga aaccatctta taatcagctg 120
<210> 10
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
aacaattacg aaccaaacct atttaaaact ccacaaagga aaccatctta taatcagctg 60
gcttcaactc caataatatt caaagagcaa gggctgactc tgccgctgta ccaatctcct 120
<210> 11
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 11
gcttcaactc caataatatt caaagagcaa gggctgactc tgccgctgta ccaatctcct 60
gtaaaagaat tagataaatt caaattagac ttaggtaagt aatgcaatat ggtagactgg 120
<210> 12
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 12
gtaaaagaat tagataaatt caaattagac ttaggtaagt aatgcaatat ggtagactgg 60
ggagaactac aaactaggaa tttaggcaaa cctgtgttaa aatcttagct cattcattaa 120
<210> 13
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 13
tccaaagaat gcaaatttat aatccagagt atatacattc tcactgaatt attgtactgt 60
ttcaggaagg aatgttccca atagtagaca taaaagtctt cgcacagtga aaactaaaat 120
<210> 14
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 14
ttcaggaagg aatgttccca atagtagaca taaaagtctt cgcacagtga aaactaaaat 60
ggatcaagca gatgatgttt cctgtccact tctaaattct tgtcttagtg aaaggtatga 120
<210> 15
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 15
ggatcaagca gatgatgttt cctgtccact tctaaattct tgtcttagtg aaaggtatga 60
tgaagctatt atattaaaat atttaaatga aacattttcc tacatatatt tgttctataa 120
<210> 16
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 16
ataagataaa ctagtttttg ccagtttttt aaaataacct aagggatttg ctttgtttta 60
ttttagtcct gttgttctac aatgtacaca tgtaacacca caaagagata agtcaggtat 120
<210> 17
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 17
ttttagtcct gttgttctac aatgtacaca tgtaacacca caaagagata agtcaggtat 60
gattaaaaac aatgcttttt attcttagaa tactagaaat gttaataaaa ataaaactta 120
<210> 18
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 18
gattaaaaac aatgcttttt attcttagaa tactagaaat gttaataaaa ataaaactta 60
acaattttcc ccttttttta cccccagtgg tatgtgggag tttgtttcat acaccaaagt 120
<210> 19
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 19
caattttccc ctttttttac ccccagtggt atgtgggagt ttgtttcata caccaaagtt 60
tgtgaaggta aatattctac ctggtttatt tttatgactt agtaattgag aatttgacaa 120
<210> 20
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 20
tgtgaaggta aatattctac ctggtttatt tttatgactt agtaattgag aatttgacaa 60
tagcgttata cctttgccct gagatttaca aatctgtacc tagcattctg cctcatacag 120
<210> 21
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 21
aaataatatc cttaatgatc agggcatttc tataaaaaat aaactatttt ctttcctccc 60
agggtcgtca gacaccaaaa catatttctg aaagtctagg agctgaggtg gatcctgata 120
<210> 22
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 22
agggtcgtca gacaccaaaa catatttctg aaagtctagg agctgaggtg gatcctgata 60
tgtcttggtc aagttcttta gctacaccac ccacccttag ttctactgtg ctcataggta 120
<210> 23
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 23
tgtcttggtc aagttcttta gctacaccac ccacccttag ttctactgtg ctcataggta 60
ataatagcaa atgtgtattt acaagaaaga gcagatgagg ttgataattg tcatctctaa 120
<210> 24
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 24
aatcaaatag tagatgtgct ttttgatgtc tgacaaaaaa taagtttttg cattctagtg 60
ataatataca atacacataa atttttatct tacagtcaga aatgaagaag catctgaaac 120
<210> 25
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 25
ataatataca atacacataa atttttatct tacagtcaga aatgaagaag catctgaaac 60
tgtatttcct catgatacta ctgctgtaag taaatatgac attgattaga ctgttgaaat 120
<210> 26
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 26
tgtatttcct catgatacta ctgctgtaag taaatatgac attgattaga ctgttgaaat 60
tgctaacaat tttggaatgc cttgttaaat tatttatctt acatttttaa tttcctaatc 120
<210> 27
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 27
ggactactac tatatgtgca ttgagagttt ttatactagt gattttaaac tataattttt 60
gcagaatgtg aaaagctatt tttccaatca tgatgaaagt ctgaagaaaa atgatagatt 120
<210> 28
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 28
gcagaatgtg aaaagctatt tttccaatca tgatgaaagt ctgaagaaaa atgatagatt 60
tatcgcttct gtgacagaca gtgaaaacac aaatcaaaga gaagctgcaa gtcatggtaa 120
<210> 29
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 29
tatcgcttct gtgacagaca gtgaaaacac aaatcaaaga gaagctgcaa gtcatggtaa 60
gtcctctgtt tagttgaact acaggttttt ttgttgttgt tgttttgatt tttttttttt 120
<210> 30
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 30
aaaggttgtg agaataatat aaattatatg gcttataaaa tattaatgtg cttctgtttt 60
atactttaac aggatttgga aaaacatcag ggaattcatt taaagtaaat agctgcaaag 120
<210> 31
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 31
atactttaac aggatttgga aaaacatcag ggaattcatt taaagtaaat agctgcaaag 60
accacattgg aaagtcaatg ccaaatgtcc tagaagatga agtatatgaa acagttgtag 120
<210> 32
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 32
accacattgg aaagtcaatg ccaaatgtcc tagaagatga agtatatgaa acagttgtag 60
atacctctga agaagatagt ttttcattat gtttttctaa atgtagaaca aaaaatctac 120
<210> 33
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 33
atacctctga agaagatagt ttttcattat gtttttctaa atgtagaaca aaaaatctac 60
aaaaagtaag aactagcaag actaggaaaa aaattttcca tgaagcaaac gctgatgaat 120
<210> 34
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 34
aaaaagtaag aactagcaag actaggaaaa aaattttcca tgaagcaaac gctgatgaat 60
gtgaaaaatc taaaaaccaa gtgaaagaaa aatactcatt tgtatctgaa gtggaaccaa 120
<210> 35
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 35
gtgaaaaatc taaaaaccaa gtgaaagaaa aatactcatt tgtatctgaa gtggaaccaa 60
atgatactga tccattagat tcaaatgtag caaatcagaa gccctttgag agtggaagtg 120
<210> 36
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 36
atgatactga tccattagat tcaaatgtag caaatcagaa gccctttgag agtggaagtg 60
acaaaatctc caaggaagtt gtaccgtctt tggcctgtga atggtctcaa ctaacccttt 120
<210> 37
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 37
acaaaatctc caaggaagtt gtaccgtctt tggcctgtga atggtctcaa ctaacccttt 60
caggtctaaa tggagcccag atggagaaaa tacccctatt gcatatttct tcatgtgacc 120
<210> 38
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 38
caggtctaaa tggagcccag atggagaaaa tacccctatt gcatatttct tcatgtgacc 60
aaaatatttc agaaaaagac ctattagaca cagagaacaa aagaaagaaa gattttctta 120
<210> 39
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 39
aaaatatttc agaaaaagac ctattagaca cagagaacaa aagaaagaaa gattttctta 60
cttcagagaa ttctttgcca cgtatttcta gcctaccaaa atcagagaag ccattaaatg 120
<210> 40
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 40
cttcagagaa ttctttgcca cgtatttcta gcctaccaaa atcagagaag ccattaaatg 60
aggaaacagt ggtaaataag agagatgaag agcagcatct tgaatctcat acagactgca 120
<210> 41
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 41
aggaaacagt ggtaaataag agagatgaag agcagcatct tgaatctcat acagactgca 60
ttcttgcagt aaagcaggca atatctggaa cttctccagt ggcttcttca tttcagggta 120
<210> 42
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 42
ttcttgcagt aaagcaggca atatctggaa cttctccagt ggcttcttca tttcagggta 60
tcaaaaagtc tatattcaga ataagagaat cacctaaaga gactttcaat gcaagttttt 120
<210> 43
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 43
tcaaaaagtc tatattcaga ataagagaat cacctaaaga gactttcaat gcaagttttt 60
caggtcatat gactgatcca aactttaaaa aagaaactga agcctctgaa agtggactgg 120
<210> 44
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 44
caggtcatat gactgatcca aactttaaaa aagaaactga agcctctgaa agtggactgg 60
aaatacatac tgtttgctca cagaaggagg actccttatg tccaaattta attgataatg 120
<210> 45
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 45
aaatacatac tgtttgctca cagaaggagg actccttatg tccaaattta attgataatg 60
gaagctggcc agccaccacc acacagaatt ctgtagcttt gaagaatgca ggtttaatat 120
<210> 46
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 46
gaagctggcc agccaccacc acacagaatt ctgtagcttt gaagaatgca ggtttaatat 60
ccactttgaa aaagaaaaca aataagttta tttatgctat acatgatgaa acatcttata 120
<210> 47
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 47
ccactttgaa aaagaaaaca aataagttta tttatgctat acatgatgaa acatcttata 60
aaggaaaaaa aataccgaaa gaccaaaaat cagaactaat taactgttca gcccagtttg 120
<210> 48
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 48
aaggaaaaaa aataccgaaa gaccaaaaat cagaactaat taactgttca gcccagtttg 60
aagcaaatgc ttttgaagca ccacttacat ttgcaaatgc tgattcaggt acctctgtct 120
<210> 49
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 49
aagcaaatgc ttttgaagca ccacttacat ttgcaaatgc tgattcaggt acctctgtct 60
tttttttttt gtaaatagta catatagttt tatagatgac gattccttct gtgttttttt 120
<210> 50
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 50
actacctttt taacttagtg aaaaatattt agtgaatgtg attgatggta ctttaatttt 60
gtcactttgt gtttttatgt ttaggtttat tgcattcttc tgtgaaaaga agctgttcac 120
<210> 51
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 51
gtcactttgt gtttttatgt ttaggtttat tgcattcttc tgtgaaaaga agctgttcac 60
agaatgattc tgaagaacca actttgtcct taactagctc ttttgggaca attctgagga 120
<210> 52
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 52
agaatgattc tgaagaacca actttgtcct taactagctc ttttgggaca attctgagga 60
aatgttctag aaatgaaaca tgttctaata atacagtaat ctctcaggat cttgattata 120
<210> 53
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 53
aatgttctag aaatgaaaca tgttctaata atacagtaat ctctcaggat cttgattata 60
aagaagcaaa atgtaataag gaaaaactac agttatttat taccccagaa gctgattctc 120
<210> 54
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 54
aagaagcaaa atgtaataag gaaaaactac agttatttat taccccagaa gctgattctc 60
tgtcatgcct gcaggaagga cagtgtgaaa atgatccaaa aagcaaaaaa gtttcagata 120
<210> 55
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 55
tgtcatgcct gcaggaagga cagtgtgaaa atgatccaaa aagcaaaaaa gtttcagata 60
taaaagaaga ggtcttggct gcagcatgtc acccagtaca acattcaaaa gtggaataca 120
<210> 56
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 56
taaaagaaga ggtcttggct gcagcatgtc acccagtaca acattcaaaa gtggaataca 60
gtgatactga ctttcaatcc cagaaaagtc ttttatatga tcatgaaaat gccagcactc 120
<210> 57
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 57
gtgatactga ctttcaatcc cagaaaagtc ttttatatga tcatgaaaat gccagcactc 60
ttattttaac tcctacttcc aaggatgttc tgtcaaacct agtcatgatt tctagaggca 120
<210> 58
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 58
ttattttaac tcctacttcc aaggatgttc tgtcaaacct agtcatgatt tctagaggca 60
aagaatcata caaaatgtca gacaagctca aaggtaacaa ttatgaatct gatgttgaat 120
<210> 59
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 59
aagaatcata caaaatgtca gacaagctca aaggtaacaa ttatgaatct gatgttgaat 60
taaccaaaaa tattcccatg gaaaagaatc aagatgtatg tgctttaaat gaaaattata 120
<210> 60
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 60
taaccaaaaa tattcccatg gaaaagaatc aagatgtatg tgctttaaat gaaaattata 60
aaaacgttga gctgttgcca cctgaaaaat acatgagagt agcatcacct tcaagaaagg 120
<210> 61
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 61
aaaacgttga gctgttgcca cctgaaaaat acatgagagt agcatcacct tcaagaaagg 60
tacaattcaa ccaaaacaca aatctaagag taatccaaaa aaatcaagaa gaaactactt 120
<210> 62
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 62
tacaattcaa ccaaaacaca aatctaagag taatccaaaa aaatcaagaa gaaactactt 60
caatttcaaa aataactgtc aatccagact ctgaagaact tttctcagac aatgagaata 120
<210> 63
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 63
caatttcaaa aataactgtc aatccagact ctgaagaact tttctcagac aatgagaata 60
attttgtctt ccaagtagct aatgaaagga ataatcttgc tttaggaaat actaaggaac 120
<210> 64
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 64
attttgtctt ccaagtagct aatgaaagga ataatcttgc tttaggaaat actaaggaac 60
ttcatgaaac agacttgact tgtgtaaacg aacccatttt caagaactct accatggttt 120
<210> 65
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 65
ttcatgaaac agacttgact tgtgtaaacg aacccatttt caagaactct accatggttt 60
tatatggaga cacaggtgat aaacaagcaa cccaagtgtc aattaaaaaa gatttggttt 120
<210> 66
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 66
tatatggaga cacaggtgat aaacaagcaa cccaagtgtc aattaaaaaa gatttggttt 60
atgttcttgc agaggagaac aaaaatagtg taaagcagca tataaaaatg actctaggtc 120
<210> 67
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 67
atgttcttgc agaggagaac aaaaatagtg taaagcagca tataaaaatg actctaggtc 60
aagatttaaa atcggacatc tccttgaata tagataaaat accagaaaaa aataatgatt 120
<210> 68
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 68
aagatttaaa atcggacatc tccttgaata tagataaaat accagaaaaa aataatgatt 60
acatgaacaa atgggcagga ctcttaggtc caatttcaaa tcacagtttt ggaggtagct 120
<210> 69
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 69
acatgaacaa atgggcagga ctcttaggtc caatttcaaa tcacagtttt ggaggtagct 60
tcagaacagc ttcaaataag gaaatcaagc tctctgaaca taacattaag aagagcaaaa 120
<210> 70
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 70
tcagaacagc ttcaaataag gaaatcaagc tctctgaaca taacattaag aagagcaaaa 60
tgttcttcaa agatattgaa gaacaatatc ctactagttt agcttgtgtt gaaattgtaa 120
<210> 71
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 71
tgttcttcaa agatattgaa gaacaatatc ctactagttt agcttgtgtt gaaattgtaa 60
ataccttggc attagataat caaaagaaac tgagcaagcc tcagtcaatt aatactgtat 120
<210> 72
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 72
ataccttggc attagataat caaaagaaac tgagcaagcc tcagtcaatt aatactgtat 60
ctgcacattt acagagtagt gtagttgttt ctgattgtaa aaatagtcat ataacccctc 120
<210> 73
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 73
ctgcacattt acagagtagt gtagttgttt ctgattgtaa aaatagtcat ataacccctc 60
agatgttatt ttccaagcag gattttaatt caaaccataa tttaacacct agccaaaagg 120
<210> 74
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 74
agatgttatt ttccaagcag gattttaatt caaaccataa tttaacacct agccaaaagg 60
cagaaattac agaactttct actatattag aagaatcagg aagtcagttt gaatttactc 120
<210> 75
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 75
cagaaattac agaactttct actatattag aagaatcagg aagtcagttt gaatttactc 60
agtttagaaa accaagctac atattgcaga agagtacatt tgaagtgcct gaaaaccaga 120
<210> 76
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 76
agtttagaaa accaagctac atattgcaga agagtacatt tgaagtgcct gaaaaccaga 60
tgactatctt aaagaccact tctgaggaat gcagagatgc tgatcttcat gtcataatga 120
<210> 77
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 77
tgactatctt aaagaccact tctgaggaat gcagagatgc tgatcttcat gtcataatga 60
atgccccatc gattggtcag gtagacagca gcaagcaatt tgaaggtaca gttgaaatta 120
<210> 78
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 78
atgccccatc gattggtcag gtagacagca gcaagcaatt tgaaggtaca gttgaaatta 60
aacggaagtt tgctggcctg ttgaaaaatg actgtaacaa aagtgcttct ggttatttaa 120
<210> 79
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 79
aacggaagtt tgctggcctg ttgaaaaatg actgtaacaa aagtgcttct ggttatttaa 60
cagatgaaaa tgaagtgggg tttaggggct tttattctgc tcatggcaca aaactgaatg 120
<210> 80
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 80
cagatgaaaa tgaagtgggg tttaggggct tttattctgc tcatggcaca aaactgaatg 60
tttctactga agctctgcaa aaagctgtga aactgtttag tgatattgag aatattagtg 120
<210> 81
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 81
tttctactga agctctgcaa aaagctgtga aactgtttag tgatattgag aatattagtg 60
aggaaacttc tgcagaggta catccaataa gtttatcttc aagtaaatgt catgattctg 120
<210> 82
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 82
aggaaacttc tgcagaggta catccaataa gtttatcttc aagtaaatgt catgattctg 60
ttgtttcaat gtttaagata gaaaatcata atgataaaac tgtaagtgaa aaaaataata 120
<210> 83
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 83
ttgtttcaat gtttaagata gaaaatcata atgataaaac tgtaagtgaa aaaaataata 60
aatgccaact gatattacaa aataatattg aaatgactac tggcactttt gttgaagaaa 120
<210> 84
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 84
aatgccaact gatattacaa aataatattg aaatgactac tggcactttt gttgaagaaa 60
ttactgaaaa ttacaagaga aatactgaaa atgaagataa caaatatact gctgccagta 120
<210> 85
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 85
ttactgaaaa ttacaagaga aatactgaaa atgaagataa caaatatact gctgccagta 60
gaaattctca taacttagaa tttgatggca gtgattcaag taaaaatgat actgtttgta 120
<210> 86
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 86
gaaattctca taacttagaa tttgatggca gtgattcaag taaaaatgat actgtttgta 60
ttcataaaga tgaaacggac ttgctattta ctgatcagca caacatatgt cttaaattat 120
<210> 87
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 87
ttcataaaga tgaaacggac ttgctattta ctgatcagca caacatatgt cttaaattat 60
ctggccagtt tatgaaggag ggaaacactc agattaaaga agatttgtca gatttaactt 120
<210> 88
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 88
ctggccagtt tatgaaggag ggaaacactc agattaaaga agatttgtca gatttaactt 60
ttttggaagt tgcgaaagct caagaagcat gtcatggtaa tacttcaaat aaagaacagt 120
<210> 89
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 89
ttttggaagt tgcgaaagct caagaagcat gtcatggtaa tacttcaaat aaagaacagt 60
taactgctac taaaacggag caaaatataa aagattttga gacttctgat acattttttc 120
<210> 90
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 90
taactgctac taaaacggag caaaatataa aagattttga gacttctgat acattttttc 60
agactgcaag tgggaaaaat attagtgtcg ccaaagagtc atttaataaa attgtaaatt 120
<210> 91
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 91
agactgcaag tgggaaaaat attagtgtcg ccaaagagtc atttaataaa attgtaaatt 60
tctttgatca gaaaccagaa gaattgcata acttttcctt aaattctgaa ttacattctg 120
<210> 92
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 92
tctttgatca gaaaccagaa gaattgcata acttttcctt aaattctgaa ttacattctg 60
acataagaaa gaacaaaatg gacattctaa gttatgagga aacagacata gttaaacaca 120
<210> 93
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 93
acataagaaa gaacaaaatg gacattctaa gttatgagga aacagacata gttaaacaca 60
aaatactgaa agaaagtgtc ccagttggta ctggaaatca actagtgacc ttccagggac 120
<210> 94
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 94
aaatactgaa agaaagtgtc ccagttggta ctggaaatca actagtgacc ttccagggac 60
aacccgaacg tgatgaaaag atcaaagaac ctactctatt gggttttcat acagctagcg 120
<210> 95
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 95
aacccgaacg tgatgaaaag atcaaagaac ctactctatt gggttttcat acagctagcg 60
ggaaaaaagt taaaattgca aaggaatctt tggacaaagt gaaaaacctt tttgatgaaa 120
<210> 96
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 96
ggaaaaaagt taaaattgca aaggaatctt tggacaaagt gaaaaacctt tttgatgaaa 60
aagagcaagg tactagtgaa atcaccagtt ttagccatca atgggcaaag accctaaagt 120
<210> 97
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 97
aagagcaagg tactagtgaa atcaccagtt ttagccatca atgggcaaag accctaaagt 60
acagagaggc ctgtaaagac cttgaattag catgtgagac cattgagatc acagctgccc 120
<210> 98
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 98
acagagaggc ctgtaaagac cttgaattag catgtgagac cattgagatc acagctgccc 60
caaagtgtaa agaaatgcag aattctctca ataatgataa aaaccttgtt tctattgaga 120
<210> 99
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 99
caaagtgtaa agaaatgcag aattctctca ataatgataa aaaccttgtt tctattgaga 60
ctgtggtgcc acctaagctc ttaagtgata atttatgtag acaaactgaa aatctcaaaa 120
<210> 100
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 100
ctgtggtgcc acctaagctc ttaagtgata atttatgtag acaaactgaa aatctcaaaa 60
catcaaaaag tatctttttg aaagttaaag tacatgaaaa tgtagaaaaa gaaacagcaa 120
<210> 101
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 101
catcaaaaag tatctttttg aaagttaaag tacatgaaaa tgtagaaaaa gaaacagcaa 60
aaagtcctgc aacttgttac acaaatcagt ccccttattc agtcattgaa aattcagcct 120
<210> 102
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 102
aaagtcctgc aacttgttac acaaatcagt ccccttattc agtcattgaa aattcagcct 60
tagcttttta cacaagttgt agtagaaaaa cttctgtgag tcagacttca ttacttgaag 120
<210> 103
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 103
tagcttttta cacaagttgt agtagaaaaa cttctgtgag tcagacttca ttacttgaag 60
caaaaaaatg gcttagagaa ggaatatttg atggtcaacc agaaagaata aatactgcag 120
<210> 104
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 104
caaaaaaatg gcttagagaa ggaatatttg atggtcaacc agaaagaata aatactgcag 60
attatgtagg aaattatttg tatgaaaata attcaaacag tactatagct gaaaatgaca 120
<210> 105
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 105
attatgtagg aaattatttg tatgaaaata attcaaacag tactatagct gaaaatgaca 60
aaaatcatct ctccgaaaaa caagatactt atttaagtaa cagtagcatg tctaacagct 120
<210> 106
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 106
aaaatcatct ctccgaaaaa caagatactt atttaagtaa cagtagcatg tctaacagct 60
attcctacca ttctgatgag gtatataatg attcaggata tctctcaaaa aataaacttg 120
<210> 107
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 107
attcctacca ttctgatgag gtatataatg attcaggata tctctcaaaa aataaacttg 60
attctggtat tgagccagta ttgaagaatg ttgaagatca aaaaaacact agtttttcca 120
<210> 108
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 108
attctggtat tgagccagta ttgaagaatg ttgaagatca aaaaaacact agtttttcca 60
aagtaatatc caatgtaaaa gatgcaaatg catacccaca aactgtaaat gaagatattt 120
<210> 109
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 109
aagtaatatc caatgtaaaa gatgcaaatg catacccaca aactgtaaat gaagatattt 60
gcgttgagga acttgtgact agctcttcac cctgcaaaaa taaaaatgca gccattaaat 120
<210> 110
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 110
gcgttgagga acttgtgact agctcttcac cctgcaaaaa taaaaatgca gccattaaat 60
tgtccatatc taatagtaat aattttgagg tagggccacc tgcatttagg atagccagtg 120
<210> 111
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 111
tgtccatatc taatagtaat aattttgagg tagggccacc tgcatttagg atagccagtg 60
gtaaaatcgt ttgtgtttca catgaaacaa ttaaaaaagt gaaagacata tttacagaca 120
<210> 112
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 112
gtaaaatcgt ttgtgtttca catgaaacaa ttaaaaaagt gaaagacata tttacagaca 60
gtttcagtaa agtaattaag gaaaacaacg agaataaatc aaaaatttgc caaacgaaaa 120
<210> 113
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 113
gtttcagtaa agtaattaag gaaaacaacg agaataaatc aaaaatttgc caaacgaaaa 60
ttatggcagg ttgttacgag gcattggatg attcagagga tattcttcat aactctctag 120
<210> 114
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 114
ttatggcagg ttgttacgag gcattggatg attcagagga tattcttcat aactctctag 60
ataatgatga atgtagcacg cattcacata aggtttttgc tgacattcag agtgaagaaa 120
<210> 115
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 115
ataatgatga atgtagcacg cattcacata aggtttttgc tgacattcag agtgaagaaa 60
ttttacaaca taaccaaaat atgtctggat tggagaaagt ttctaaaata tcaccttgtg 120
<210> 116
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 116
ttttacaaca taaccaaaat atgtctggat tggagaaagt ttctaaaata tcaccttgtg 60
atgttagttt ggaaacttca gatatatgta aatgtagtat agggaagctt cataagtcag 120
<210> 117
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 117
atgttagttt ggaaacttca gatatatgta aatgtagtat agggaagctt cataagtcag 60
tctcatctgc aaatacttgt gggattttta gcacagcaag tggaaaatct gtccaggtat 120
<210> 118
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 118
tctcatctgc aaatacttgt gggattttta gcacagcaag tggaaaatct gtccaggtat 60
cagatgcttc attacaaaac gcaagacaag tgttttctga aatagaagat agtaccaagc 120
<210> 119
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 119
cagatgcttc attacaaaac gcaagacaag tgttttctga aatagaagat agtaccaagc 60
aagtcttttc caaagtattg tttaaaagta acgaacattc agaccagctc acaagagaag 120
<210> 120
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 120
aagtcttttc caaagtattg tttaaaagta acgaacattc agaccagctc acaagagaag 60
aaaatactgc tatacgtact ccagaacatt taatatccca aaaaggcttt tcatataatg 120
<210> 121
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 121
aaaatactgc tatacgtact ccagaacatt taatatccca aaaaggcttt tcatataatg 60
tggtaaattc atctgctttc tctggattta gtacagcaag tggaaagcaa gtttccattt 120
<210> 122
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 122
tggtaaattc atctgctttc tctggattta gtacagcaag tggaaagcaa gtttccattt 60
tagaaagttc cttacacaaa gttaagggag tgttagagga atttgattta atcagaactg 120
<210> 123
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 123
tagaaagttc cttacacaaa gttaagggag tgttagagga atttgattta atcagaactg 60
agcatagtct tcactattca cctacgtcta gacaaaatgt atcaaaaata cttcctcgtg 120
<210> 124
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 124
agcatagtct tcactattca cctacgtcta gacaaaatgt atcaaaaata cttcctcgtg 60
ttgataagag aaacccagag cactgtgtaa actcagaaat ggaaaaaacc tgcagtaaag 120
<210> 125
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 125
ttgataagag aaacccagag cactgtgtaa actcagaaat ggaaaaaacc tgcagtaaag 60
aatttaaatt atcaaataac ttaaatgttg aaggtggttc ttcagaaaat aatcactcta 120
<210> 126
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 126
aatttaaatt atcaaataac ttaaatgttg aaggtggttc ttcagaaaat aatcactcta 60
ttaaagtttc tccatatctc tctcaatttc aacaagacaa acaacagttg gtattaggaa 120
<210> 127
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 127
ttaaagtttc tccatatctc tctcaatttc aacaagacaa acaacagttg gtattaggaa 60
ccaaagtgtc acttgttgag aacattcatg ttttgggaaa agaacaggct tcacctaaaa 120
<210> 128
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 128
ccaaagtgtc acttgttgag aacattcatg ttttgggaaa agaacaggct tcacctaaaa 60
acgtaaaaat ggaaattggt aaaactgaaa ctttttctga tgttcctgtg aaaacaaata 120
<210> 129
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 129
acgtaaaaat ggaaattggt aaaactgaaa ctttttctga tgttcctgtg aaaacaaata 60
tagaagtttg ttctacttac tccaaagatt cagaaaacta ctttgaaaca gaagcagtag 120
<210> 130
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 130
tagaagtttg ttctacttac tccaaagatt cagaaaacta ctttgaaaca gaagcagtag 60
aaattgctaa agcttttatg gaagatgatg aactgacaga ttctaaactg ccaagtcatg 120
<210> 131
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 131
aaattgctaa agcttttatg gaagatgatg aactgacaga ttctaaactg ccaagtcatg 60
ccacacattc tctttttaca tgtcccgaaa atgaggaaat ggttttgtca aattcaagaa 120
<210> 132
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 132
ccacacattc tctttttaca tgtcccgaaa atgaggaaat ggttttgtca aattcaagaa 60
ttggaaaaag aagaggagag ccccttatct tagtgggtaa gtgttcattt ttacctttcg 120
<210> 133
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 133
ttggaaaaag aagaggagag ccccttatct tagtgggtaa gtgttcattt ttacctttcg 60
tgttgccaat cactattttt aaagtgttta ttcagtagac ttggtatgct aacaattaag 120
<210> 134
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 134
ggtctataga cttttgagaa ataaaactga tattatttgc cttaaaaaca tatatgaaat 60
atttcttttt aggagaaccc tcaatcaaaa gaaacttatt aaatgaattt gacaggataa 120
<210> 135
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 135
atttcttttt aggagaaccc tcaatcaaaa gaaacttatt aaatgaattt gacaggataa 60
tagaaaatca agaaaaatcc ttaaaggctt caaaaagcac tccagatggt aaaattagct 120
<210> 136
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 136
tagaaaatca agaaaaatcc ttaaaggctt caaaaagcac tccagatggt aaaattagct 60
ttttatttat atctgttctc cctctatagg tatggtatat aatattctga cctcaggtga 120
<210> 137
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 137
gtctcaaatt ttttgtgtat ttacagtaac atggatattc tcttagattt taactaatat 60
gtaatataaa ataattgttt cctaggcaca ataaaagatc gaagattgtt tatgcatcat 120
<210> 138
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 138
gtaatataaa ataattgttt cctaggcaca ataaaagatc gaagattgtt tatgcatcat 60
gtttctttag agccgattac ctgtgtaccc tttcggtaag acatgtttaa atttttctaa 120
<210> 139
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 139
gtttctttag agccgattac ctgtgtaccc tttcggtaag acatgtttaa atttttctaa 60
attctaatac agtatgagaa aagtctcgtt tttataaatg aacatttcta aaaataatga 120
<210> 140
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 140
caaatgaggg tctgcaacaa aggcatattc ctaaatattt atatgtgtac tagtcaataa 60
acttatatat tttctcccca ttgcagcaca actaaggaac gtcaagagat acagaatcca 120
<210> 141
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 141
acttatatat tttctcccca ttgcagcaca actaaggaac gtcaagagat acagaatcca 60
aattttaccg cacctggtca agaatttctg tctaaatctc atttgtatga acatctgact 120
<210> 142
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 142
aattttaccg cacctggtca agaatttctg tctaaatctc atttgtatga acatctgact 60
ttggaaaaat cttcaagcaa tttagcagtt tcaggacatc cattttatca agtttctgct 120
<210> 143
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 143
ttggaaaaat cttcaagcaa tttagcagtt tcaggacatc cattttatca agtttctgct 60
acaagaaatg aaaaaatgag acacttgatt actacaggca gaccaaccaa agtctttgtt 120
<210> 144
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 144
acaagaaatg aaaaaatgag acacttgatt actacaggca gaccaaccaa agtctttgtt 60
ccacctttta aaactaaatc acattttcac agagttgaac agtgtgttag gaatattaac 120
<210> 145
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 145
ccacctttta aaactaaatc acattttcac agagttgaac agtgtgttag gaatattaac 60
ttggaggaaa acagacaaaa gcaaaacatt gatggacatg gctctgatga tagtaaaaat 120
<210> 146
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 146
ttggaggaaa acagacaaaa gcaaaacatt gatggacatg gctctgatga tagtaaaaat 60
aagattaatg acaatgagat tcatcagttt aacaaaaaca actccaatca agcagtagct 120
<210> 147
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 147
aagattaatg acaatgagat tcatcagttt aacaaaaaca actccaatca agcagtagct 60
gtaactttca caaagtgtga agaagaacct ttaggtattg tatgacaatt tgtgtgatga 120
<210> 148
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 148
gtaactttca caaagtgtga agaagaacct ttaggtattg tatgacaatt tgtgtgatga 60
atttttgcct ttcagttaga tatttccgtt gttaaataat gtcctgatgg ttttccccct 120
<210> 149
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 149
ggattacagg cgtgagccac tgtgcctggc caggggttgt gctttttaaa tttcaatttt 60
atttttgcta agtatttatt ctttgataga tttaattaca agtcttcaga atgccagaga 120
<210> 150
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 150
atttttgcta agtatttatt ctttgataga tttaattaca agtcttcaga atgccagaga 60
tatacaggat atgcgaatta agaagaaaca aaggcaacgc gtctttccac agccaggcag 120
<210> 151
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 151
tatacaggat atgcgaatta agaagaaaca aaggcaacgc gtctttccac agccaggcag 60
tctgtatctt gcaaaaacat ccactctgcc tcgaatctct ctgaaagcag cagtaggagg 120
<210> 152
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 152
tctgtatctt gcaaaaacat ccactctgcc tcgaatctct ctgaaagcag cagtaggagg 60
ccaagttccc tctgcgtgtt ctcataaaca ggtatgtgtt tgtctacaat actgatggct 120
<210> 153
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 153
ccaagttccc tctgcgtgtt ctcataaaca ggtatgtgtt tgtctacaat actgatggct 60
tttatgacag agtgtaattt tatttcatta actagtatct acaaatggct ttgtttaaag 120
<210> 154
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 154
ttttggtttg ttataattgt ttttattgtg tgatacatgt ttactttaaa ttgtttttct 60
tttttgtgtg tgtttatttt gtgtagctgt atacgtatgg cgtttctaaa cattgcataa 120
<210> 155
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 155
tttttgtgtg tgtttatttt gtgtagctgt atacgtatgg cgtttctaaa cattgcataa 60
aaattaacag caaaaatgca gagtcttttc agtttcacac tgaagattat tttggtaagg 120
<210> 156
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 156
aaattaacag caaaaatgca gagtcttttc agtttcacac tgaagattat tttggtaagg 60
aaagtttatg gactggaaaa ggaatacagt tggctgatgg tggatggctc ataccctcca 120
<210> 157
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 157
aaagtttatg gactggaaaa ggaatacagt tggctgatgg tggatggctc ataccctcca 60
atgatggaaa ggctggaaaa gaagaatttt ataggtactc tatgcaaaaa gattgtgtgt 120
<210> 158
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 158
atgatggaaa ggctggaaaa gaagaatttt ataggtactc tatgcaaaaa gattgtgtgt 60
taacttttat gtattccctc atccctcttt cttctcttaa ctgtctctcg aactaaaaag 120
<210> 159
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 159
agttgttgaa ttcagtatca tcctatgtgg tttttatgat aatattctac ttttatttgt 60
tcagggctct gtgtgacact ccaggtgtgg atccaaagct tatttctaga atttgggttt 120
<210> 160
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 160
tcagggctct gtgtgacact ccaggtgtgg atccaaagct tatttctaga atttgggttt 60
ataatcacta tagatggatc atatggaaac tggcagctat ggaatgtgcc tttcctaagg 120
<210> 161
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 161
ataatcacta tagatggatc atatggaaac tggcagctat ggaatgtgcc tttcctaagg 60
aatttgctaa tagatgccta agcccagaaa gggtgcttct tcaactaaaa tacaggcaag 120
<210> 162
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 162
aatttgctaa tagatgccta agcccagaaa gggtgcttct tcaactaaaa tacaggcaag 60
tttaaagcat tacattacgt aatcatatac ggcagtatgg ttaaggtttc tgtgtagtct 120
<210> 163
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 163
tttaaacagt ggaattctag agtcacactt cctaaaatat gcatttttgt tttcactttt 60
agatatgata cggaaattga tagaagcaga agatcggcta taaaaaagat aatggaaagg 120
<210> 164
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 164
agatatgata cggaaattga tagaagcaga agatcggcta taaaaaagat aatggaaagg 60
gatgacacag ctgcaaaaac acttgttctc tgtgtttctg acataatttc attgagcgca 120
<210> 165
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 165
gatgacacag ctgcaaaaac acttgttctc tgtgtttctg acataatttc attgagcgca 60
aatatatctg aaacttctag caataaaact agtagtgcag atacccaaaa agtggccatt 120
<210> 166
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 166
aatatatctg aaacttctag caataaaact agtagtgcag atacccaaaa agtggccatt 60
attgaactta cagatgggtg gtatgctgtt aaggcccagt tagatcctcc cctcttagct 120
<210> 167
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 167
attgaactta cagatgggtg gtatgctgtt aaggcccagt tagatcctcc cctcttagct 60
gtcttaaaga atggcagact gacagttggt cagaagatta ttcttcatgg agcagaactg 120
<210> 168
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 168
gtcttaaaga atggcagact gacagttggt cagaagatta ttcttcatgg agcagaactg 60
gtgggctctc ctgatgcctg tacacctctt gaagccccag aatctcttat gttaaaggta 120
<210> 169
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 169
gtgggctctc ctgatgcctg tacacctctt gaagccccag aatctcttat gttaaaggta 60
aattaatttg cactcttggt aaaaatcagt cattgattca gttaaattct agaagtttta 120
<210> 170
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 170
ctcttatgat atctgtaata gaattgaata catatttaac tactaaatca atatatttat 60
taatttgtcc agatttctgc taacagtact cggcctgctc gctggtatac caaacttgga 120
<210> 171
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 171
taatttgtcc agatttctgc taacagtact cggcctgctc gctggtatac caaacttgga 60
ttctttcctg accctagacc ttttcctctg cccttatcat cgcttttcag tgatggagga 120
<210> 172
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 172
ttctttcctg accctagacc ttttcctctg cccttatcat cgcttttcag tgatggagga 60
aatgttggtt gtgttgatgt aattattcaa agagcatacc ctatacaggt atgatgtatt 120
<210> 173
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 173
aatgttggtt gtgttgatgt aattattcaa agagcatacc ctatacaggt atgatgtatt 60
cttgaaactt accatatatt tctttctttt gatacaatta atttgtttgt ttgtttgaga 120
<210> 174
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 174
cactgtgcct ggcctgatac aattaacttg aatgttatat atgtgacttt tttggtgtgt 60
gtaacacatt attacagtgg atggagaaga catcatctgg attatacata tttcgcaatg 120
<210> 175
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 175
gtaacacatt attacagtgg atggagaaga catcatctgg attatacata tttcgcaatg 60
aaagagagga agaaaaggaa gcagcaaaat atgtggaggc ccaacaaaag agactagaag 120
<210> 176
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 176
aaagagagga agaaaaggaa gcagcaaaat atgtggaggc ccaacaaaag agactagaag 60
ccttattcac taaaattcag gaggaatttg aagaacatga aggtaaaatt agttatatgg 120
<210> 177
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 177
ccttattcac taaaattcag gaggaatttg aagaacatga aggtaaaatt agttatatgg 60
tacacattgt tatttctaat atgagaacaa agtcttagag actttgaatt taacattttt 120
<210> 178
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 178
cttctttggg tgttttatgc ttggttcttt agttttagtt gcttttgaat ttacagttta 60
gtgaattaat aatccttttg ttttcttaga aaacacaaca aaaccatatt taccatcacg 120
<210> 179
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 179
gtgaattaat aatccttttg ttttcttaga aaacacaaca aaaccatatt taccatcacg 60
tgcactaaca agacagcaag ttcgtgcttt gcaagatggt gcagagcttt atgaagcagt 120
<210> 180
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 180
tgcactaaca agacagcaag ttcgtgcttt gcaagatggt gcagagcttt atgaagcagt 60
gaagaatgca gcagacccag cttaccttga ggtgagagag taagaggaca tataatgagg 120
<210> 181
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 181
gaagaatgca gcagacccag cttaccttga ggtgagagag taagaggaca tataatgagg 60
cttgatgatt attcaaggtg agaagctgtt ttagactctc tggccatcac aggaaggagt 120
<210> 182
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 182
tgtttaaagc catctagtta caatagatgg aacttttttg ttctgattgc tttttattcc 60
aatatcttaa atggtcacag ggttatttca gtgaagagca gttaagagcc ttgaataatc 120
<210> 183
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 183
aatatcttaa atggtcacag ggttatttca gtgaagagca gttaagagcc ttgaataatc 60
acaggcaaat gttgaatgat aagaaacaag ctcagatcca gttggaaatt aggaaggcca 120
<210> 184
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 184
acaggcaaat gttgaatgat aagaaacaag ctcagatcca gttggaaatt aggaaggcca 60
tggaatctgc tgaacaaaag gaacaaggtt tatcaaggga tgtcacaacc gtgtggaagt 120
<210> 185
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 185
tggaatctgc tgaacaaaag gaacaaggtt tatcaaggga tgtcacaacc gtgtggaagt 60
tgcgtattgt aagctattca aaaaaagaaa aagattcagg taagtatgta aatgctttgt 120
<210> 186
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 186
tgcgtattgt aagctattca aaaaaagaaa aagattcagg taagtatgta aatgctttgt 60
ttttatcagt tttattaact taaaaaatga ccttactaac aaaatgatta taaatccaga 120
<210> 187
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 187
attttgaaac aaacatttaa atgataatca cttcttccat tgcatctttc tcatctttct 60
ccaaacagtt atactgagta tttggcgtcc atcatcagat ttatattctc tgttaacaga 120
<210> 188
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 188
ccaaacagtt atactgagta tttggcgtcc atcatcagat ttatattctc tgttaacaga 60
aggaaagaga tacagaattt atcatcttgc aacttcaaaa tctaaaagta aatctgaaag 120
<210> 189
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 189
aggaaagaga tacagaattt atcatcttgc aacttcaaaa tctaaaagta aatctgaaag 60
agctaacata cagttagcag cgacaaaaaa aactcagtat caacaactac cggtacaaac 120
<210> 190
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 190
agctaacata cagttagcag cgacaaaaaa aactcagtat caacaactac cggtacaaac 60
ctttcattgt aatttttcag ttttgataag tgcttgttag tttatggaat ctccatatgt 120
<210> 191
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 191
aatttttcag ttttgataag tgcttgttag tttatggaat ctccatatgt tgaatttttg 60
ttttgttttc tgtaggtttc agatgaaatt ttatttcaga tttaccagcc acgggagccc 120
<210> 192
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 192
ttttgttttc tgtaggtttc agatgaaatt ttatttcaga tttaccagcc acgggagccc 60
cttcacttca gcaaattttt agatccagac tttcagccat cttgttctga ggtggaccta 120
<210> 193
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 193
cttcacttca gcaaattttt agatccagac tttcagccat cttgttctga ggtggaccta 60
ataggatttg tcgtttctgt tgtgaaaaaa acaggtaatg cacaatatag ttaatttttt 120
<210> 194
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 194
ataggatttg tcgtttctgt tgtgaaaaaa acaggtaatg cacaatatag ttaatttttt 60
ttattgattc ttttaaaaaa cattgtcttt taaaatctct tatgattagt tggagctacc 120
<210> 195
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 195
ataggcatat tagagtttcc tttcttgcat cttaaaattc atctaacaca tctataataa 60
cattcttttc ttttttttcc attctaggac ttgccccttt cgtctatttg tcagacgaat 120
<210> 196
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 196
cattcttttc ttttttttcc attctaggac ttgccccttt cgtctatttg tcagacgaat 60
gttacaattt actggcaata aagttttgga tagaccttaa tgaggacatt attaagcctc 120
<210> 197
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 197
gttacaattt actggcaata aagttttgga tagaccttaa tgaggacatt attaagcctc 60
atatgttaat tgctgcaagc aacctccagt ggcgaccaga atccaaatca ggccttctta 120
<210> 198
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 198
atatgttaat tgctgcaagc aacctccagt ggcgaccaga atccaaatca ggccttctta 60
ctttatttgc tggagatttt tctgtgtttt ctgctagtcc aaaagagggc cactttcaag 120
<210> 199
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 199
ctttatttgc tggagatttt tctgtgtttt ctgctagtcc aaaagagggc cactttcaag 60
agacattcaa caaaatgaaa aatactgttg aggtaaggtt acttttcagc atcaccacac 120
<210> 200
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 200
agacattcaa caaaatgaaa aatactgttg aggtaaggtt acttttcagc atcaccacac 60
attttggtat ttttctattt tgacagtcca gtatcaagga aatagctttt atacaaattg 120
<210> 201
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 201
ttaaaggaaa tacttttgga aacataaata tgtgggtttg caatttataa agcagctttt 60
ccacttattt tcttagaata ttgacatact ttgcaatgaa gcagaaaaca agcttatgca 120
<210> 202
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 202
ccacttattt tcttagaata ttgacatact ttgcaatgaa gcagaaaaca agcttatgca 60
tatactgcat gcaaatgatc ccaagtggtc caccccaact aaagactgta cttcagggcc 120
<210> 203
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 203
tatactgcat gcaaatgatc ccaagtggtc caccccaact aaagactgta cttcagggcc 60
gtacactgct caaatcattc ctggtacagg aaacaagctt ctggtaagtt aatgtaaact 120
<210> 204
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 204
gtacactgct caaatcattc ctggtacagg aaacaagctt ctggtaagtt aatgtaaact 60
caaggaatat tataagaagt atatatggag gccatcgtat attctgttgt atacctagta 120
<210> 205
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 205
aatattttca atgaaaagtt actttgattt agttttttat gttactacat aattatgata 60
ggctacgttt tcattttttt atcagatgtc ttctcctaat tgtgagatat attatcaaag 120
<210> 206
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 206
ggctacgttt tcattttttt atcagatgtc ttctcctaat tgtgagatat attatcaaag 60
tcctttatca ctttgtatgg ccaaaaggaa gtctgtttcc acacctgtct cagcccagat 120
<210> 207
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 207
tcctttatca ctttgtatgg ccaaaaggaa gtctgtttcc acacctgtct cagcccagat 60
gacttcaaag tcttgtaaag gggagaaaga gattgatgac caaaagaact gcaaaaagag 120
<210> 208
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 208
gacttcaaag tcttgtaaag gggagaaaga gattgatgac caaaagaact gcaaaaagag 60
aagagccttg gatttcttga gtagactgcc tttacctcca cctgttagtc ccatttgtac 120
<210> 209
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 209
aagagccttg gatttcttga gtagactgcc tttacctcca cctgttagtc ccatttgtac 60
atttgtttct ccggctgcac agaaggcatt tcagccacca aggagttgtg gcaccaaata 120
<210> 210
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 210
atttgtttct ccggctgcac agaaggcatt tcagccacca aggagttgtg gcaccaaata 60
cgaaacaccc ataaagaaaa aagaactgaa ttctcctcag atgactccat ttaaaaaatt 120
<210> 211
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 211
cgaaacaccc ataaagaaaa aagaactgaa ttctcctcag atgactccat ttaaaaaatt 60
caatgaaatt tctcttttgg aaagtaattc aatagctgac gaagaacttg cattgataaa 120
<210> 212
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 212
caatgaaatt tctcttttgg aaagtaattc aatagctgac gaagaacttg cattgataaa 60
tacccaagct cttttgtctg gttcaacagg agaaaaacaa tttatatctg tcagtgaatc 120
<210> 213
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 213
tacccaagct cttttgtctg gttcaacagg agaaaaacaa tttatatctg tcagtgaatc 60
cactaggact gctcccacca gttcagaaga ttatctcaga ctgaaacgac gttgtactac 120
<210> 214
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 214
cactaggact gctcccacca gttcagaaga ttatctcaga ctgaaacgac gttgtactac 60
atctctgatc aaagaacagg agagttccca ggccagtacg gaagaatgtg agaaaaataa 120
<210> 215
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 215
atctctgatc aaagaacagg agagttccca ggccagtacg gaagaatgtg agaaaaataa 60
gcaggacaca attacaacta aaaaatatat ctaagcattt gcaaaggcga caataaatta 120
<210> 216
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 216
gcaggacaca attacaacta aaaaatatat ctaagcattt gcaaaggcga caataaatta 60
ttgacgctta acctttccag tttataagac tggaatataa tttcaaacca cacattagta 120
<210> 217
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 217
taaattataa ataacatcca agctacatga aacaaaatat gaagcttgag tatatgtgca 60
tattatcccc tcaaaagtga caatttaatg actacaaagt caacatactg catcataagg 120
<210> 218
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 218
tattatcccc tcaaaagtga caatttaatg actacaaagt caacatactg catcataagg 60
gataatggta aaaatttgtg ttatttattc agcaatcatt taatgagacc ctattgccca 120
<210> 219
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 219
gataatggta aaaatttgtg ttatttattc agcaatcatt taatgagacc ctattgccca 60
taaagagcat ttgccagttc tatgaatgat gcaaacagcg aacacaatac aagtcaatat 120
<210> 220
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 220
gcctggaaag gccactttgt aagctcattc ttggggtcct gtggctctgt acctgtggct 60
ggctgcagtc agtagtggct gtgggggatc tggggtatca ggtaggtgtc cagctcctgg 120
<210> 221
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 221
ggctgcagtc agtagtggct gtgggggatc tggggtatca ggtaggtgtc cagctcctgg 60
cactggtaga gtgctacact gtccaacacc cactctcggg tcaccacagg tgcctcacac 120
<210> 222
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 222
cactggtaga gtgctacact gtccaacacc cactctcggg tcaccacagg tgcctcacac 60
atctgcccaa ttgctggaga cagagaacac aagcagagat tagtgtcaat tcattctcct 120
<210> 223
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 223
atctgcccaa ttgctggaga cagagaacac aagcagagat tagtgtcaat tcattctcct 60
ggactaggct ctaatcaatc gactccaggg tcctggttgt atgagttctt aggattaatg 120
<210> 224
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 224
actgtgctac tcaagcacca ggtaatgagt gataaaccaa acccatgcaa aaggacccca 60
tatagcacag gtacatgcag gcaccttacc atggaagcca ttgtcctctg tccaggcatc 120
<210> 225
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 225
tatagcacag gtacatgcag gcaccttacc atggaagcca ttgtcctctg tccaggcatc 60
tggctgcaca accacaattg ggtggacacc ctggatcccc aggaaggaaa gagcattcaa 120
<210> 226
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 226
tggctgcaca accacaattg ggtggacacc ctggatcccc aggaaggaaa gagcattcaa 60
agtgtcaaag taggactact ggaactgtca cttcatcatt ttttttgttt gtttttgaga 120
<210> 227
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 227
tccataggga ctgacaggtg ccagtcttgc tcacaggaga gaatattgtg tcctccctct 60
ctgacagggc acccaatact tactgtgcca agggtgaatg atgaaagctc cttcaccaca 120
<210> 228
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 228
ctgacagggc acccaatact tactgtgcca agggtgaatg atgaaagctc cttcaccaca 60
gaagcaccac acagctgtac catccattcc agttgatcta aaatggacat ttagatgtaa 120
<210> 229
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 229
gaagcaccac acagctgtac catccattcc agttgatcta aaatggacat ttagatgtaa 60
aatcactgca gtaatctgca tacttaaccc aggccctcta ccctacactc tccggatgaa 120
<210> 230
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 230
gaggaacccc catcgtggga tcttgcttat aatactccac tatgtaagac aaaggctggt 60
gctggaactc tggggttctc ccaggctctt acctgtgggc atgttggtga agggcccata 120
<210> 231
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 231
gctggaactc tggggttctc ccaggctctt acctgtgggc atgttggtga agggcccata 60
gcaacagatt tctagccccc tgaagatctg gaagaagaga ggaagagaga gggacagggg 120
<210> 232
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 232
gcaacagatt tctagccccc tgaagatctg gaagaagaga ggaagagaga gggacagggg 60
aatggagaga aggaaaatct agttataaaa gaatattggc ttttattcaa aaaacagact 120
<210> 233
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 233
cggcccatct ctgcaaaggg gagtggaata cagagtggtg gggtgagatt tttgtcaact 60
tgagggaggg agctttacct ttctgtcctg ggattctctt gctcgctttg gaccttggtg 120
<210> 234
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 234
tgagggaggg agctttacct ttctgtcctg ggattctctt gctcgctttg gaccttggtg 60
gtttcttcca ttgaccacat ctcctctgac ttcaaaatca tgctgaaaga aaccaaacac 120
<210> 235
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 235
gtttcttcca ttgaccacat ctcctctgac ttcaaaatca tgctgaaaga aaccaaacac 60
aacccatcag gataagagaa agagaagctt ccttcaatgg aagtggagca gacacgtcat 120
<210> 236
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 236
tgttaaggaa agtggtgcat tgatggaagg aagcaaatac atttttaact atatgactga 60
atgaatatct ctggttagtt tgtaacatca agtacttacc tcattcagca tttttctttc 120
<210> 237
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 237
atgaatatct ctggttagtt tgtaacatca agtacttacc tcattcagca tttttctttc 60
tttaatagac tgggtcaccc ctaaagagat catagaaaag acaggttaca tacagcagaa 120
<210> 238
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 238
tttaatagac tgggtcaccc ctaaagagat catagaaaag acaggttaca tacagcagaa 60
gaacgtgctc ttttcacgga gatagagagg tcagcgattc acaaaagagc acaggaagaa 120
<210> 239
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 239
accttaggtg ttaaacgtta ggtgtaaaaa tgcaattctg aggtgttaaa gggaggaggg 60
gagaaatagt attatactta cagaaatagc taactaccca ttttcctccc gcaattccta 120
<210> 240
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 240
gagaaatagt attatactta cagaaatagc taactaccca ttttcctccc gcaattccta 60
gaaaatattt cagtgtccgt tcacacacaa actcagcatc tgcagaatga aaaacactca 120
<210> 241
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 241
gaaaatattt cagtgtccgt tcacacacaa actcagcatc tgcagaatga aaaacactca 60
aaggattaga agttgaaaac aaaatcagga agtgctgtcc taagaagcta aagagcctca 120
<210> 242
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 242
gcatgcgcca ccgtgcctcg cctcatgtgg ttttatgcag cagatgcaag gtattctgta 60
aaggttcttg gtatacctgt tttcataaca acatgagtag tctcttcagt aattagatta 120
<210> 243
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 243
aaggttcttg gtatacctgt tttcataaca acatgagtag tctcttcagt aattagatta 60
gttaaagtga tgtggtgttt tctggcaaac ttgtacacga gcatctgaaa ttaaatcaaa 120
<210> 244
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 244
gttaaagtga tgtggtgttt tctggcaaac ttgtacacga gcatctgaaa ttaaatcaaa 60
tattccatta tcatgagtta cctctagcac acagctcaga atactagtta ttccaccatg 120
<210> 245
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 245
attaattgac aatacctaca taaaactctt tccagaatgt tgttaagtct tagtcattag 60
ggagatacat atggatacac tcacaaattc ttctggggtc aggccagaca ccaccatgga 120
<210> 246
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 246
ggagatacat atggatacac tcacaaattc ttctggggtc aggccagaca ccaccatgga 60
cattcttttg ttgacccttt ctgttgaagc tgtcaattct ggcttctccc tgctcacact 120
<210> 247
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 247
cattcttttg ttgacccttt ctgttgaagc tgtcaattct ggcttctccc tgctcacact 60
ttcttccatt gcattatacc cagcagtatc agtagtatga gcagcagctg gactctgggc 120
<210> 248
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 248
ttcttccatt gcattatacc cagcagtatc agtagtatga gcagcagctg gactctgggc 60
agattctgca actttcaatt ggggaacttt caatgcagag gttgaagatg gtatgttgcc 120
<210> 249
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 249
agattctgca actttcaatt ggggaacttt caatgcagag gttgaagatg gtatgttgcc 60
aacacgagct gactctgggg ctctgtcttc agaaggatca gattcagggt catcagagaa 120
<210> 250
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 250
aacacgagct gactctgggg ctctgtcttc agaaggatca gattcagggt catcagagaa 60
gaggctgatt ccagattcca ggtaaggggt tccctctgaa aggaatggga gaagtttaat 120
<210> 251
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 251
gaggctgatt ccagattcca ggtaaggggt tccctctgaa aggaatggga gaagtttaat 60
ttacacaacg atgaatgttg aattacaaag ttctggtctc tgttaagaat taaaaagacc 120
<210> 252
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 252
aaaagttggt taaccagaat atctttatgt aggattcaga gtaaaatcaa agtgtttgtt 60
ccaatacagc agatgaaata ttacctagat cttgccttgg caagtaagat gtttccgtca 120
<210> 253
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 253
ccaatacagc agatgaaata ttacctagat cttgccttgg caagtaagat gtttccgtca 60
aatcgtgtgg cccagactct tccagctgtt gctcctccac atcaacaacc ttaatgagct 120
<210> 254
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 254
aatcgtgtgg cccagactct tccagctgtt gctcctccac atcaacaacc ttaatgagct 60
cctcttgaga tgggtagttt ctattctgaa gactcccaga gcaactgtgc atgtaccacc 120
<210> 255
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 255
cctcttgaga tgggtagttt ctattctgaa gactcccaga gcaactgtgc atgtaccacc 60
tatcatctaa tgatgggcat ttagaagggg atgacctaga aagataaatg gaaggagaaa 120
<210> 256
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 256
tatcatctaa tgatgggcat ttagaagggg atgacctaga aagataaatg gaaggagaaa 60
accatcgcca ccaattgtga aaggacaaat catacttgct gggcagccaa agcataaatg 120
<210> 257
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 257
aagtatggtg aaaaaaatta acaatcagag ttcaatataa ataaagatgt cagataccac 60
agcatcttta cattgatgtt tcttaccttt ccactcctgg ttctttattt ttactggtag 120
<210> 258
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 258
agcatcttta cattgatgtt tcttaccttt ccactcctgg ttctttattt ttactggtag 60
aactatctgc agacacctca aacttgtcag cagaaaggcc ttctggattc tggcttatag 120
<210> 259
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 259
aactatctgc agacacctca aacttgtcag cagaaaggcc ttctggattc tggcttatag 60
ggtattcact acttttctgt gaagttaata ctgctttaaa tggaatgaga aaacaaatct 120
<210> 260
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 260
ggtattcact acttttctgt gaagttaata ctgctttaaa tggaatgaga aaacaaatct 60
actttactgc tttgttctga tagtgataat tcaggttaga atactgattt ttttcaaaag 120
<210> 261
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 261
aaggagataa aggggaagga aagaattttg cttaagatat cagtgtttgg ccaacaatac 60
acaccttttt ctgatgtgct ttgttctgga tttcgcaggt cctcaagggc agaagagtca 120
<210> 262
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 262
acaccttttt ctgatgtgct ttgttctgga tttcgcaggt cctcaagggc agaagagtca 60
cttatgatgg aagggtagct gttagaaggc tggctcccat gctgttctaa cacagcttct 120
<210> 263
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 263
cttatgatgg aagggtagct gttagaaggc tggctcccat gctgttctaa cacagcttct 60
agttcagcca tttcctgctg gagctttatc aggttatgtt gcatggtatc cctctgcttc 120
<210> 264
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 264
agttcagcca tttcctgctg gagctttatc aggttatgtt gcatggtatc cctctgcttc 60
aaaaacgata aatggcacca agaaaatgaa atactttgag aagctttcca ttaaatgaaa 120
<210> 265
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 265
agaatgtggg atacatacta ctgaatgcaa aggacaccac acacacgcat gtgcacacac 60
acacacgctt tttacctgag tggttaaaat gtcactctga gaggatagcc ctgagcagtc 120
<210> 266
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 266
acacacgctt tttacctgag tggttaaaat gtcactctga gaggatagcc ctgagcagtc 60
ttcagagacg cttgtttcac tctcacaccc agatgctgct tcaccttaaa taacaaaaac 120
<210> 267
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 267
ttcagagacg cttgtttcac tctcacaccc agatgctgct tcaccttaaa taacaaaaac 60
agaggttcag atgtaaaagc agactataaa cgctgcaact tgctgtgtct ttttcttctc 120
<210> 268
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 268
tttgtaaaat gtgctcccca aaagcataaa catttagctc acttctataa atagactggg 60
gcaaacacaa aaacctggtt ccaataccta agtttgaatc catgctttgc tcttcttgat 120
<210> 269
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 269
gcaaacacaa aaacctggtt ccaataccta agtttgaatc catgctttgc tcttcttgat 60
tattttcttc caagcccgtt cctctttctt catcatctga aaccaattcc ttgtcactca 120
<210> 270
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 270
tattttcttc caagcccgtt cctctttctt catcatctga aaccaattcc ttgtcactca 60
gaccaactcc ctggctttca gactgatgcc tcatttgttt ggaagaacca atcaagaaag 120
<210> 271
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 271
gaccaactcc ctggctttca gactgatgcc tcatttgttt ggaagaacca atcaagaaag 60
gatcctgggt gtttgtattt gcagtcaagt cttccaattc actgcactgt gaagaaaaca 120
<210> 272
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 272
gatcctgggt gtttgtattt gcagtcaagt cttccaattc actgcactgt gaagaaaaca 60
agctagcaga acattttgtt tcctcactaa ggtgatgttc ctgagatgcc tttgccaata 120
<210> 273
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 273
agctagcaga acattttgtt tcctcactaa ggtgatgttc ctgagatgcc tttgccaata 60
ttacctggtt actgcagtca tttaagctat tcttcaatga taataaattc tcctctgtgt 120
<210> 274
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 274
ttacctggtt actgcagtca tttaagctat tcttcaatga taataaattc tcctctgtgt 60
tcttagacag acactcggta gcaacggtgc tatgcctagt agactgagaa ggtatattgt 120
<210> 275
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 275
tcttagacag acactcggta gcaacggtgc tatgcctagt agactgagaa ggtatattgt 60
ttactttacc aaataacaag tgttggaagc agggaagctc ttcatcctca ctagataagt 120
<210> 276
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 276
ttactttacc aaataacaag tgttggaagc agggaagctc ttcatcctca ctagataagt 60
tctcttctga ggactctaat ttcttggccc ctcttcggta accctgagcc aaatgtgtat 120
<210> 277
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 277
tctcttctga ggactctaat ttcttggccc ctcttcggta accctgagcc aaatgtgtat 60
gggtgaaagg gctaggactc ctgctaagct ctcctttctg gacgcttttg ctaaaaacag 120
<210> 278
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 278
gggtgaaagg gctaggactc ctgctaagct ctcctttctg gacgcttttg ctaaaaacag 60
cagaactttc cttaatgtca ttttcagcaa aactagtatc ttcctttatt tcaccatcat 120
<210> 279
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 279
cagaactttc cttaatgtca ttttcagcaa aactagtatc ttcctttatt tcaccatcat 60
ctaacaggtc atcaggtgtc tcagaacaaa cctgagatgc atgactactt cccataggct 120
<210> 280
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 280
ctaacaggtc atcaggtgtc tcagaacaaa cctgagatgc atgactactt cccataggct 60
gttctaagtt atctgaaatc agatatggag agaaatctgt attaacagtc tgaactactt 120
<210> 281
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 281
gttctaagtt atctgaaatc agatatggag agaaatctgt attaacagtc tgaactactt 60
cttcatattc ttgctttttt atttcaggat gcttacaatt acttccagga agactttgtt 120
<210> 282
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 282
cttcatattc ttgctttttt atttcaggat gcttacaatt acttccagga agactttgtt 60
tatagacctc aggttgcaaa acccctaatc taagcatagc attcaatttt ggccctctgt 120
<210> 283
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 283
tatagacctc aggttgcaaa acccctaatc taagcatagc attcaatttt ggccctctgt 60
ttctacctag ttctgcttga atgttttcat cactggaacc tatttcatta atactggagc 120
<210> 284
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 284
ttctacctag ttctgcttga atgttttcat cactggaacc tatttcatta atactggagc 60
ccacttcatt agtactggaa cctacttcat taatattgct tgagctggct tctttaaaaa 120
<210> 285
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 285
ccacttcatt agtactggaa cctacttcat taatattgct tgagctggct tctttaaaaa 60
cattttctct aatgttatta cggctaattg tgctcactgt acttggaatg ttctcatttc 120
<210> 286
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 286
cattttctct aatgttatta cggctaattg tgctcactgt acttggaatg ttctcatttc 60
ccatttctct ttcaggtgac attgaatgtt cctcaaagtt ttcctctagc agatttttct 120
<210> 287
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 287
ccatttctct ttcaggtgac attgaatgtt cctcaaagtt ttcctctagc agatttttct 60
tacatttagt tttaacaaat gacttgatgg gaaaaagtgg tggtatacga tatgggtttt 120
<210> 288
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 288
tacatttagt tttaacaaat gacttgatgg gaaaaagtgg tggtatacga tatgggtttt 60
gtaaaagtcc atgtttattt ggagtaatga gtccagtttc gttgcctctg aactgagatg 120
<210> 289
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 289
gtaaaagtcc atgtttattt ggagtaatga gtccagtttc gttgcctctg aactgagatg 60
atagacaaaa cctagagcct cctttgatac tacatttggc attatcaact ggcttatctt 120
<210> 290
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 290
atagacaaaa cctagagcct cctttgatac tacatttggc attatcaact ggcttatctt 60
tctgaccaac cacaggaaag cctgcagtga tattaactgt ctgtacaggc ttgatattag 120
<210> 291
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 291
tctgaccaac cacaggaaag cctgcagtga tattaactgt ctgtacaggc ttgatattag 60
actcattctt tccttgattt tcttcctttt gttcacattc aaaagtgact tttggacttt 120
<210> 292
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 292
actcattctt tccttgattt tcttcctttt gttcacattc aaaagtgact tttggacttt 60
gtttctttaa ggacccagag tgggcagaga atgttgcaca ttcctcttct gcatttcctg 120
<210> 293
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 293
gtttctttaa ggacccagag tgggcagaga atgttgcaca ttcctcttct gcatttcctg 60
gatttgaaaa cggagcaaat gactggcgct ttgaaacctt gaatgtattc tgcaaatact 120
<210> 294
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 294
gatttgaaaa cggagcaaat gactggcgct ttgaaacctt gaatgtattc tgcaaatact 60
gagcatcaag ttcactttct tccatttcta tgcttgtttc ccgactgtgg ttaacttcat 120
<210> 295
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 295
gagcatcaag ttcactttct tccatttcta tgcttgtttc ccgactgtgg ttaacttcat 60
gtcccaatgg atacttaaag ccttctgtgt catttctatt atctttggaa caaccatgaa 120
<210> 296
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 296
gtcccaatgg atacttaaag ccttctgtgt catttctatt atctttggaa caaccatgaa 60
ttagtccctt ggggttttca aatgctgcac actgactcac acatttattt ggttctgttt 120
<210> 297
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 297
ttagtccctt ggggttttca aatgctgcac actgactcac acatttattt ggttctgttt 60
ttgccttccc tagagtgcta acttccagta acgagatact ttcctgagtg ccataatcag 120
<210> 298
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 298
ttgccttccc tagagtgcta acttccagta acgagatact ttcctgagtg ccataatcag 60
taccaggtac caatgaaata ctgctactct ctacagatct ttcagtttgc aaaacccttt 120
<210> 299
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 299
taccaggtac caatgaaata ctgctactct ctacagatct ttcagtttgc aaaacccttt 60
ctccacttaa catgagatct ttggggtctt cagcattatt agacacttta actgtttcta 120
<210> 300
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 300
ctccacttaa catgagatct ttggggtctt cagcattatt agacacttta actgtttcta 60
gtttctcttc tttttcttct cttggaaggc taggattgac aaattcttta agttcactgg 120
<210> 301
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 301
gtttctcttc tttttcttct cttggaaggc taggattgac aaattcttta agttcactgg 60
tatttgaaca cttagtaaaa gaaccaggtg catttgttaa cttcagctct gggaaagtat 120
<210> 302
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 302
tatttgaaca cttagtaaaa gaaccaggtg catttgttaa cttcagctct gggaaagtat 60
cgctgtcatg tcttttactt gtctgttcat ttggcttgtt actcttcttg gctccagttg 120
<210> 303
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 303
cgctgtcatg tcttttactt gtctgttcat ttggcttgtt actcttcttg gctccagttg 60
caggttcttt accttccatg agttgtaggt ttctgctgtg cctgactggc atttggttgt 120
<210> 304
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 304
caggttcttt accttccatg agttgtaggt ttctgctgtg cctgactggc atttggttgt 60
actttttttt ctttatctct tcactgctag aacaactatc aatttgcaat tcagtacaat 120
<210> 305
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 305
actttttttt ctttatctct tcactgctag aacaactatc aatttgcaat tcagtacaat 60
taggtgggct tagatttcta ctgactacta gttcaagcgc atgaatatgc ctggtagaag 120
<210> 306
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 306
taggtgggct tagatttcta ctgactacta gttcaagcgc atgaatatgc ctggtagaag 60
acttcctcct cagcctattc tttttaggtg cttttgaatt gtggatattt aattcgagtt 120
<210> 307
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 307
acttcctcct cagcctattc tttttaggtg cttttgaatt gtggatattt aattcgagtt 60
ccatattgct tatactgctg cttataggtt cagctttcgt tttgaaagca gattcttttt 120
<210> 308
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 308
ccatattgct tatactgctg cttataggtt cagctttcgt tttgaaagca gattcttttt 60
cgagtgattc tattgggtta ggatttttct cattctgaat agaatcacct tttgttttat 120
<210> 309
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 309
cgagtgattc tattgggtta ggatttttct cattctgaat agaatcacct tttgttttat 60
tctcatgacc actattagta atattcatca cttgaccatt ctgctccgtt tggttagttc 120
<210> 310
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 310
tctcatgacc actattagta atattcatca cttgaccatt ctgctccgtt tggttagttc 60
cctgatttat catttcagga gtcttttgaa ctgccaaatc tgctttcttg ataaaatcct 120
<210> 311
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 311
cctgatttat catttcagga gtcttttgaa ctgccaaatc tgctttcttg ataaaatcct 60
caggatgaag gcctgatgta ggtctccttt tacgctttaa tttatttgtg aggggacgct 120
<210> 312
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 312
caggatgaag gcctgatgta ggtctccttt tacgctttaa tttatttgtg aggggacgct 60
cttgtattat ctgtggctca gtaacaaatg ctcctataat tagattttca gttacatggc 120
<210> 313
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 313
cttgtattat ctgtggctca gtaacaaatg ctcctataat tagattttca gttacatggc 60
ttaagttggg gaggcttgcc ttcttccgat aggttttccc aaatattttg tcttcaatat 120
<210> 314
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 314
ttaagttggg gaggcttgcc ttcttccgat aggttttccc aaatattttg tcttcaatat 60
tactctctac tgatttggag tgaactcttt cacttttaca tattaaagcc tcatgaggat 120
<210> 315
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 315
tactctctac tgatttggag tgaactcttt cacttttaca tattaaagcc tcatgaggat 60
cactggccag taagtctatt ttctctgaag aaccagaata ttcatctacc tcatttagaa 120
<210> 316
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 316
cactggccag taagtctatt ttctctgaag aaccagaata ttcatctacc tcatttagaa 60
cgtccaatac atcagctact ttggcatttg attcagactc cccatcatgt gagtcatcag 120
<210> 317
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 317
cgtccaatac atcagctact ttggcatttg attcagactc cccatcatgt gagtcatcag 60
aacctaacag ttcatcactt ctggaaaacc actcattaac tttctgaatg ctgctattta 120
<210> 318
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 318
aacctaacag ttcatcactt ctggaaaacc actcattaac tttctgaatg ctgctattta 60
gtgttatcca aggaacatct tcagtatctc taggattctc tgagcatggc agtttctgct 120
<210> 319
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 319
gtgttatcca aggaacatct tcagtatctc taggattctc tgagcatggc agtttctgct 60
tattccattc ttttctctca cacaggggat cagcattcag atctaccttt ttttctgtgc 120
<210> 320
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 320
tattccattc ttttctctca cacaggggat cagcattcag atctaccttt ttttctgtgc 60
tgggagtccg cctatcatta catgtttcct tacttccagc ccatctgtta tgttggctcc 120
<210> 321
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 321
tgggagtccg cctatcatta catgtttcct tacttccagc ccatctgtta tgttggctcc 60
ttgctaagcc aggctgtttg cttttattac agaattcagc cttttctaca ttcattctgt 120
<210> 322
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 322
ttgctaagcc aggctgtttg cttttattac agaattcagc cttttctaca ttcattctgt 60
ctttagtgag taataaactg ctgttctcat gctgtaatga gctggcatga gtatttgtgc 120
<210> 323
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 323
ctttagtgag taataaactg ctgttctcat gctgtaatga gctggcatga gtatttgtgc 60
cacatggctc cacatgcaag tttgaaacag aactaccctg atacttttct ggatgcctct 120
<210> 324
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 324
cacatggctc cacatgcaag tttgaaacag aactaccctg atacttttct ggatgcctct 60
cagctgcacg cttctcagtg gtgttcaaat cattattact gggttgatga tgttcagtat 120
<210> 325
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 325
cagctgcacg cttctcagtg gtgttcaaat cattattact gggttgatga tgttcagtat 60
ttgttacatc cgtctcagaa aattcacaag cagctgaaaa tatacaaaaa taacaaggta 120
<210> 326
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 326
ttgttacatc cgtctcagaa aattcacaag cagctgaaaa tatacaaaaa taacaaggta 60
ctcaaaaact gaattgtcat taaaaaaata catacttcat acaccttgga ggtggaaatc 120
<210> 327
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 327
tcttcagaat aatctaatta cagtactgta tctacccact ctcttttcag tgcctgttaa 60
gttggcaaac tttgccatta cccttttttg cagaatccaa actgatttca tccctggttc 120
<210> 328
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 328
gttggcaaac tttgccatta cccttttttg cagaatccaa actgatttca tccctggttc 60
cttgaggggt gatttgtaac aattcttgat ctcccacact atagggaaaa gacagagtcc 120
<210> 329
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 329
cttgaggggt gatttgtaac aattcttgat ctcccacact atagggaaaa gacagagtcc 60
taataagaaa cactagttac atgtatgcag aactgtcaaa tgaccaagat caaacatttt 120
<210> 330
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 330
aagttaaaat atttttaaaa agagagaaac atcaatcctt aatattaact aaataggaaa 60
ataccagctt catagacaaa ggttctcttt gactcacctg caataagttg ccttattaac 120
<210> 331
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 331
ataccagctt catagacaaa ggttctcttt gactcacctg caataagttg ccttattaac 60
ggtatcttca gaagaatcag atcctaaaaa atttcccccc aaaaaataaa tcaataaaag 120
<210> 332
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 332
ggtatcttca gaagaatcag atcctaaaaa atttcccccc aaaaaataaa tcaataaaag 60
ttttcttaat taaaagggtt aaaaaaatgt acttgttgaa aaacagatat tcaactagaa 120
<210> 333
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 333
tggcaaaact ataagataag gaatccagca attattatta aatacttaaa aaacctgaga 60
cccttaccca attcaatgta gacagacgtc ttttgaggtt gtatccgctg ctttgtcctc 120
<210> 334
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 334
cccttaccca attcaatgta gacagacgtc ttttgaggtt gtatccgctg ctttgtcctc 60
agagttctca cagttccaag gttagagagt tggacactga gactggtttc ctgctaaaca 120
<210> 335
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 335
agagttctca cagttccaag gttagagagt tggacactga gactggtttc ctgctaaaca 60
gtatggtaaa gaacagtcaa gcaattgttg gccagttctg tgcttttcct cctgaagaga 120
<210> 336
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 336
agcaagactc catctcaaaa aaaaaaaaga aaaaaaaaag aaaagaagaa gaagaagaag 60
aagaaaacaa atggttttac caaggaagga ttttcgggtt cactctgtag aagtcttttg 120
<210> 337
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 337
aagaaaacaa atggttttac caaggaagga ttttcgggtt cactctgtag aagtcttttg 60
gcacggtttc tgtagcccat actttggatg atagaaactt catcttttag atgttcagga 120
<210> 338
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 338
gcacggtttc tgtagcccat actttggatg atagaaactt catcttttag atgttcagga 60
gagttatttt ccttttttgc aaaattatag ctgtttgcat ctgtaaaata caagggaaaa 120
<210> 339
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 339
gagttatttt ccttttttgc aaaattatag ctgtttgcat ctgtaaaata caagggaaaa 60
cattatgttt gcagttagag aaaaatgtat gaattataat caaagaaacc aagagaaacc 120
<210> 340
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 340
gaaagtaatt gtgcaaactt cctgagtttt catggacagc acttgagtgt cattcttggg 60
atattcaaca cttacactcc aaacctgtgt caagctgaaa agcacaaatg attttcaata 120
<210> 341
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 341
atattcaaca cttacactcc aaacctgtgt caagctgaaa agcacaaatg attttcaata 60
gctcttcaac aagttgacta aatctcgtac tttcttgtag gctcctgaaa ttaaattgtt 120
<210> 342
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 342
gctcttcaac aagttgacta aatctcgtac tttcttgtag gctcctgaaa ttaaattgtt 60
tgagaaacac actcagcaag tgattatcaa ccttttaagg acactaaaat aagaaaagac 120
<210> 343
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 343
aggaacgcta tgttattaaa taatttctac tttttcctac tgtggttgct tccaacctag 60
catcattacc aaattatata ccttttggtt atatcattct tacataaagg acactgtgaa 120
<210> 344
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 344
catcattacc aaattatata ccttttggtt atatcattct tacataaagg acactgtgaa 60
ggccctttct tctggttgag aagtttcagc atgcaaaatc tataaattat aaagaaagaa 120
<210> 345
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 345
ggccctttct tctggttgag aagtttcagc atgcaaaatc tataaattat aaagaaagaa 60
agaacaattt aatttacttc cttttgtaga aagaatactc aaaaggcaaa tagccatgaa 120
<210> 346
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 346
aaagtaaagc ttctataaag ttaggtgttt cctgggttat gaaggacaaa aacaaaagct 60
aataatggag ccacataaca cattcaaact tacttgcaaa atatgtggtc acactttgtg 120
<210> 347
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 347
aataatggag ccacataaca cattcaaact tacttgcaaa atatgtggtc acactttgtg 60
gagacaggtt ccttgatcaa ctccagacta gcagggtagg gggggagaaa aagaaaataa 120
<210> 348
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 348
gagacaggtt ccttgatcaa ctccagacta gcagggtagg gggggagaaa aagaaaataa 60
atgaggctca ataatttatt taaaaataaa gctattctta gtgaataagt tcaactttga 120
<210> 349
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 349
gtatgtaagg tcaattctgt tcatttgcat aggagataat cataggaatc ccaaattaat 60
acactcttgt gctgacttac cagatgggac actctaagat tttctgcata gcattaatga 120
<210> 350
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 350
acactcttgt gctgacttac cagatgggac actctaagat tttctgcata gcattaatga 60
cattttgtac ttcttcaacg cgaagagcag ataaatccat ttctttctgt tccaatgaac 120
<210> 351
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 351
cattttgtac ttcttcaacg cgaagagcag ataaatccat ttctttctgt tccaatgaac 60
tttaacacat tagaaaaaca tatatatata tctttttaaa aggtttataa aatgacaact 120
<210> 352
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 352
ggacactcag tgcccccttc ctgatcctca gcgcttccct cgcgacctac aaactgcccc 60
cctccccagg gttcacaacg ccttacgcct ctcaggttcc gcccctaccc cccgtcaaag 120
<210> 353
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 353
cctccccagg gttcacaacg ccttacgcct ctcaggttcc gcccctaccc cccgtcaaag 60
aatacccatc tgtcagcttc ggaaatccac tctcccacgc cagtacccca gagcatcact 120
<210> 354
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 354
aatacccatc tgtcagcttc ggaaatccac tctcccacgc cagtacccca gagcatcact 60
tgggccccct gtccctttcc cgggactcta ctacctttac ccagagcaga gggtgaaggc 120
<210> 355
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 355
caggtggaca gtccgattca gttccattca ttcactgagc acttcctgta cacctggcac 60
gggggatgcc accaagatca agacaggact atgacagcca tgctccctca ggaatcagat 120
<210> 356
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 356
attcttcccc gcttaccatc cgtgcccacc ccatcctcct ctcacctcct actcttttta 60
tttaccttgt tcgttccaga aatgcaccac acgtaagcac tctcccacct ggtcttacaa 120
<210> 357
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 357
gcgccttgct ctccgcttcg tccccatctc tccgcaggcg gatcaagccc ctgcggccgg 60
tggcgggcga agtgacccca caggctggca ggggctcctg ggggtctctg ccgtcccagg 120
<210> 358
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 358
tacagttagg tagaatgaat gagtctagag atctaattta cagcataacg actacagtta 60
acaatactgt attttatact gaaaagtttc aaagagaaat ttcaggtgct ctcaccgcac 120
<210> 359
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 359
gacttgtgtg acatgccacg ggttctcatt agtccactcc tagtgagaca cccctccccc 60
acaaaaaggg gacaaagcat attgttctga tttatagggt aaataaatca caggttagtt 120
<210> 360
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 360
agttaagtgt atgaagtcaa atggactaga attcaaacct gggccttact accaggcaca 60
ttacttaact acttgattca gtatcctcat ctgacaatac tgaccaattt acaggattac 120
<210> 361
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 361
ttacttaact acttgattca gtatcctcat ctgacaatac tgaccaattt acaggattac 60
tgtaagatta aacatggtat tgcttgaaaa cccaaagcag aatgcctaac tcataaaatg 120
<210> 362
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 362
tgtaagatta aacatggtat tgcttgaaaa cccaaagcag aatgcctaac tcataaaatg 60
tttggtaagt ggcagctgtt attagtaagg atcacagacg tatttctttg cgaattagac 120
<210> 363
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 363
aggattactg attatcaaac taaaatggtc cctaaggaac caagcggagc ccaccctttt 60
cctaatccaa ggttgcttct gtttactgct caagcacctt ctggaagcag caaggccccc 120
<210> 364
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 364
attatcaaac taaaatggtc cctaaggaac caagcggagc ccaccctttt cctaatccaa 60
ggttgcttct gtttactgct caagcacctt ctggaagcag caaggccccc atgggagcaa 120
<210> 365
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 365
cagattttcg ccaagcaaat tcgagccccg ccccttccct gggtctccat ttcccgcctc 60
cggcccggcc tttgggctcc gccttcagct caagacttaa cttccctccc agctgtccca 120
<210> 366
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 366
gggcacgctg tatttgcctt acttaagccc ctggtaattg ctgtattccg aagacatgct 60
gatgggaatt accaggcggc gttggtctct aactggagcc ctctgtcccc actagccacg 120
<210> 367
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 367
aaggaatgca tccctgtgta agtgcatttt ggtcttctgt tttgcagact tatttaccaa 60
gcattggagg aatatcgtag gtaaaaatgc ctattggatc caaagagagg ccaacatttt 120
<210> 368
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 368
ttatacaaag tttccaaata ttgataaatt gcattaaact attttaaaaa tctcattcat 60
taataccacc atggatgtca gaaaagtctt ttaagattgg gtagaaatga gccactggaa 120
<210> 369
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 369
tgggtagaaa tgagccactg gaaattctaa ttttcatttg aaagttcaca ttttgtcatt 60
gacaacaaac tgttttcctt gcagcaacaa gatcacttca ttgatttgtg agaaaatgtc 120
<210> 370
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 370
gacaacaaac tgttttcctt gcagcaacaa gatcacttca ttgatttgtg agaaaatgtc 60
taccaaatta tttaagttga aataactttg tcagctgttc tttcaagtaa aaatgacttt 120
<210> 371
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 371
taccaaatta tttaagttga aataactttg tcagctgttc tttcaagtaa aaatgacttt 60
tcattgaaaa aattgcttgt tcagatcaca gctcaacatg agtgcttttc taggcagtat 120
<210> 372
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 372
ttatgccaaa aaaacctagg acttgttttg atgttaatta aactaaacta tatttctgca 60
agctatcaca gaggacagag attattttac cgatatacta taagtatcat gatttggaag 120
<210> 373
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 373
gctaaacctt gaaggatagg tgagagatta agaaatttga agatgtggta gagtgataat 60
gttctaggca gagggaacaa catgaggaag aatatgtagt gtgttcagga aatagcaagt 120
<210> 374
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 374
gttctaggca gagggaacaa catgaggaag aatatgtagt gtgttcagga aatagcaagt 60
aattcaggtt ggctttggtt gttttgtgtc tgaaagggac caatagacaa ggcaaaaagg 120
<210> 375
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 375
aattcaggtt ggctttggtt gttttgtgtc tgaaagggac caatagacaa ggcaaaaagg 60
cagactaaag gcaggcattg aatgccaagc taaagaaatt gaatttgttt ggttggttgg 120
<210> 376
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 376
cctcagattg ctcacagcct agtaggggga aaaagaacag tgtatgatca aactcttcag 60
ggaacacata ggggggcaaa cacttaatct taccttaggg atcactacag ttttctggag 120
<210> 377
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 377
ggaacacata ggggggcaaa cacttaatct taccttaggg atcactacag ttttctggag 60
gaggtagttt ctaaatggaa gcctgaaaga gttgttccag gtcaagaaaa gcaaagaagg 120
<210> 378
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 378
gaggtagttt ctaaatggaa gcctgaaaga gttgttccag gtcaagaaaa gcaaagaagg 60
ggaaacagct tgtacaaagt cctagaggtt aaagaaaaca ttctttcagg atatgcaaat 120
<210> 379
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 379
aagttgttgt tcttgtgatg ttgaattggc tggttttctg tatattctgt gattttttaa 60
gtaacaaaaa taacagtggt gaaaagcagt aagtcagtcc ttgaattatc aatttaaaat 120
<210> 380
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 380
ttagtaactg acaataattt tattctatta agtgtagatt ggaataaata caaatacatt 60
tagtggtagt ccagtggtgt caagcattat gttttagtac gatgtgatta acgtagaata 120
<210> 381
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 381
ttgtctaaga agatactggg ccagatcatt gtttctcaaa ttgcagatta tgacctgttc 60
atagttgtga aacttatttt gtgagtcgta tatgctcttt ttaaatgaaa tgaaaattct 120
<210> 382
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 382
atagttgtga aacttatttt gtgagtcgta tatgctcttt ttaaatgaaa tgaaaattct 60
gagtacatca catgtagtta gggtttagaa aataaaaaat acaatatatc tagttaaatt 120
<210> 383
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 383
gagtacatca catgtagtta gggtttagaa aataaaaaat acaatatatc tagttaaatt 60
tggacttcag gtaaacagcg aataattttg agatatactt aacactaaaa aattattcat 120
<210> 384
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 384
tcatttcagg ggatccctta ctgaagtttt cgtttaggct ctatgctttc tggcgtaata 60
tgtagctgtc aatcaaaaca acctgttcat gttttctacc cacaaatgta ataccttttc 120
<210> 385
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 385
tgtagctgtc aatcaaaaca acctgttcat gttttctacc cacaaatgta ataccttttc 60
tacttctatg gtgcactgtg tggccacaac atttgcagtt tgaggtgtga cagcaaaacc 120
<210> 386
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 386
aaataatgat ttattgctgc tgttgttaca taattttctt aaatatttta aagattgcat 60
actgttactg ctctatttct gcatctccgt ggtgtaactc tgtcctcttt gttgttgcaa 120
<210> 387
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 387
actgttactg ctctatttct gcatctccgt ggtgtaactc tgtcctcttt gttgttgcaa 60
cagttcactt agcaactaaa ctgtatgttt acaaagtgat tttatctccc tatgagaaga 120
<210> 388
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 388
cagttcactt agcaactaaa ctgtatgttt acaaagtgat tttatctccc tatgagaaga 60
ctttagtgaa tagctcagtg aatagtagag ttggtgagac cacagtacag aactgtttga 120
<210> 389
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 389
agaactttct actatattag aagaatcagg aagtcagttt gaatttactc agtttagaaa 60
accaagctac atattgcaga agagtacatt tgaagtgcct gaaaaccaga tgactatctt 120
<210> 390
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 390
gggggagtat ggtttgatat acagatacac agattcagta ttcgtataca gatttgatat 60
cttggtatac agattcgata tctctgaatc tgtataccaa gaaatcatgt tttaagggtc 120
<210> 391
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 391
ttatttatag caacccattc tgttcttgtg catactgaag tatattgact ttccacctag 60
ggaaaaaaaa aacaataact cagacttgta aatgctttca acggtgttac tacttaattt 120
<210> 392
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 392
ggaaaaaaaa aacaataact cagacttgta aatgctttca acggtgttac tacttaattt 60
ccctcatttc tgtaacatat aagtgtataa cttagtcagc ttctggttac tggaacagta 120
<210> 393
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 393
ccctcatttc tgtaacatat aagtgtataa cttagtcagc ttctggttac tggaacagta 60
caggtcactg ttaaacaatt aaaccacttt tataataatc taacacctcc taaagccttg 120
<210> 394
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 394
ctcacaactc aatcatttaa gtgtgttttc ttgagaaacg cactgaagta tgcaagcata 60
atataccaac agtacaaata tcaacagtga aaaggacata cataacattt tactaataag 120
<210> 395
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 395
atataccaac agtacaaata tcaacagtga aaaggacata cataacattt tactaataag 60
acagttttga cagcttggat tccctaaaat ggttgtagat acctaacagg attccactga 120
<210> 396
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 396
acagttttga cagcttggat tccctaaaat ggttgtagat acctaacagg attccactga 60
tcatttcttg agaatcattg tcctataata tatacataat aatctaaatt tacaatatca 120
<210> 397
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 397
gattccactg atcatttctt gagaatcatt gtcctataat atatacataa taatctaaat 60
ttacaatatc agtattaact actgacaata aaactactaa ggaaaatgta agaattgttt 120
<210> 398
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 398
taagaatgtt tagtcaaaat actgtgttca aatgtcactc aaaataattc ttccggatgt 60
ggttaccaat ttgataatta ggttacattc ctttttttcc atttgttttc aattttagga 120
<210> 399
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 399
ggttaccaat ttgataatta ggttacattc ctttttttcc atttgttttc aattttagga 60
tttgtctttt cttatttaat tttacatttg aataaataaa acattacata gttcattcat 120
<210> 400
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 400
tttgtctttt cttatttaat tttacatttg aataaataaa acattacata gttcattcat 60
cagaactaca aaaaggtata cttagagttt ttattcaccc acctcttgct tactataggt 120
<210> 401
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 401
taaccaattt ccaactagtg gacttattga agatttaatt aggttccagt tacatactga 60
gaatgaacaa tatctaaagc ttagctttta aaccttcata agactaaatt ttaaatttgg 120
<210> 402
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 402
aagatgaatt gagaggattc tgaattgaag gtgggctgga gggaggggac accaggcaca 60
attgagggaa agatactaaa atgaaagatc agatacaaat ctgtatgtca agcagtgaga 120
<210> 403
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 403
attgagggaa agatactaaa atgaaagatc agatacaaat ctgtatgtca agcagtgaga 60
cctagctcct tcccacactt ggttcccaaa tgcaggccct ctaggcatga gactggaaga 120
<210> 404
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 404
cctagctcct tcccacactt ggttcccaaa tgcaggccct ctaggcatga gactggaaga 60
tttttttttc ctagggaata tgcctgaccc aatagaaaag accaaaaaat actgacagtt 120
<210> 405
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 405
agggaatatg cctgacccaa tagaaaagac caaaaaatac tgacagttga ggatactcag 60
atgaaacagt atagccagtc accagaccag gaagttaact gttgacatgc acagagcttc 120
<210> 406
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 406
gattatgtca tttctttact cagaactatt ccgtggtgtg ccatctcaga gtagagacta 60
aaagcccttg tcatggtgta cagattcttc atgatctggc tgctttgcta tttttccagt 120
<210> 407
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 407
attatgtcat ttctttactc agaactattc cgtggtgtgc catctcagag tagagactaa 60
aagcccttgt catggtgtac agattcttca tgatctggct gctttgctat ttttccagtc 120
<210> 408
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 408
tgtaggaaca accctaggag aaaaatcggg catgaggata aaggatattt tcattgttag 60
gtgataattt aagcaatgga aatgactcac attagcaagg gaaagtgtct aaggaagaca 120
<210> 409
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 409
gtgataattt aagcaatgga aatgactcac attagcaagg gaaagtgtct aaggaagaca 60
tccagttttg gagacttttt ttgaggaatc aggaagaggt aaaaccagta aaagatgaaa 120
<210> 410
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 410
tccagttttg gagacttttt ttgaggaatc aggaagaggt aaaaccagta aaagatgaaa 60
gaggtacagt gatggtgaga attttaaaag aaggaaaatg taaactgtca tagctattag 120
<210> 411
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 411
caactttatt gagaacagtt tctgtagagt agtgggagaa atgagagttt attgagtaga 60
gattgaggaa gtgaaaatag ctacattacc tattgaagaa ggttgactgt ggagtgtaac 120
<210> 412
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 412
acacttgatt actacaggca gaccaaccaa agtctttgtt ccacctttta aaactaaatc 60
acattttcac agagttgaac agtgtgttag gaatattaac ttggaggaaa acagacaaaa 120
<210> 413
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 413
taatattgat gacattaata gctaactttg agcattttct aggtgtaaga tgctcttcta 60
agcacttcac atgcattagg tatatcttgc ttaatcctca cagtcacctt gaaagaaagg 120
<210> 414
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 414
agcacttcac atgcattagg tatatcttgc ttaatcctca cagtcacctt gaaagaaagg 60
cactgttact ttgtttccat tttgcaaatg agagaactga agcatagaga gggttaagta 120
<210> 415
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 415
cactgttact ttgtttccat tttgcaaatg agagaactga agcatagaga gggttaagta 60
actgccccaa agtcacttaa ctagtaagtg gaagtgctat gattccaaag caaagagtct 120
<210> 416
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 416
ttatggtaga ttcctccccc gacacacact tacttcatgt tttctttcat tatatatttt 60
aatggataca aaatataaat aaacactaaa agttaaacag aaatatttga atatcaataa 120
<210> 417
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 417
aatggataca aaatataaat aaacactaaa agttaaacag aaatatttga atatcaataa 60
tgccaaataa ctagaaaatc tcagagctct aaaacagcaa caatttagaa actatataac 120
<210> 418
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 418
tgccaaataa ctagaaaatc tcagagctct aaaacagcaa caatttagaa actatataac 60
ctctttttat tgtagttttt acagaaacat aatttaaagc tttttgttat cagagatata 120
<210> 419
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 419
gtattatttt ctcgtactaa atagactgca taaggtagaa gttaagaatg attgccctgt 60
agtctaagtg gaaatgtgga ggctttcgtt agttttttct gataattcag caaatctcta 120
<210> 420
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 420
agatggacac acagagacca cttaagctat tgcagaaata catgtgagag gtggttggtt 60
ggaccaggga agtggcagtg gaattggtgg aaagcagttg gactctgggg tattttgaaa 120
<210> 421
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 421
aggcatttcc tgatcgttgg ccttgtaggc agcaaggggt aggagctagt gcacaaatgg 60
tagaagaggg caaagtgtaa ggatgcagat gcttggaaga gggcaaagtg tagggataca 120
<210> 422
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 422
tagaagaggg caaagtgtaa ggatgcagat gcttggaaga gggcaaagtg tagggataca 60
gatgctgtga gtggatagat atgagggtgg gagcttatgg aagttctctt ttgattactt 120
<210> 423
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 423
gatgctgtga gtggatagat atgagggtgg gagcttatgg aagttctctt ttgattactt 60
ctgttgtctt agtgctaaga gtatgaatga gaaaggagga gttagagatt tgagaacaga 120
<210> 424
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 424
gaatagttgt agttgttgaa ttcagtatca tcctatgtgg tttttatgat aatattctac 60
ttttatttgt tcagggctct gtgtgacact ccaggtgtgg atccaaagct tatttctaga 120
<210> 425
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 425
ttatgttgct tagaacatca agcttgcagt ggctttcatt ttcttgtttt tgtttttttt 60
taaatcaaat caatgcatgt gcataatttg gaaactcaag taatataaga catataacca 120
<210> 426
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 426
taaatcaaat caatgcatgt gcataatttg gaaactcaag taatataaga catataacca 60
aaaaaaagca gttgctagca caaccctccc catcctcatt catggtccca agaggcaatc 120
<210> 427
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 427
aaaaaaagca gttgctagca caaccctccc catcctcatt catggtccca agaggcaatc 60
gtttccagtc tgtttgacta ttttttagct tgtactgctg tcttctttct ctcttcagtt 120
<210> 428
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 428
ttctcttaat tttctgtgtc cttacacagg agctataaaa atcctgccca atatgttaaa 60
atttctccat tggttccatt tctgtttctt ggagaacccc tggacctctt catatgctta 120
<210> 429
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 429
tcaattagag cataatcata ccaagaaagt atttcaagta acttaaaaaa tgttttatgt 60
ccctagtggt atatacccca agaacaacaa tagcaacaac aactataaaa tgaaacaaaa 120
<210> 430
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 430
cacctggcta cttgggatag gaaagaaaat ctggtgtttc aactacgttt gtacaaaatg 60
tcaaccattt cttcctattt tcagccccac catatgctcc tgccttcaca ggtacctgtt 120
<210> 431
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 431
tcaaccattt cttcctattt tcagccccac catatgctcc tgccttcaca ggtacctgtt 60
gcctccaatt tctgagtgtt ttcctttaat tattttgttt catgttaact ccttacaaca 120
<210> 432
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 432
gcctccaatt tctgagtgtt ttcctttaat tattttgttt catgttaact ccttacaaca 60
agtttggtgg ctgaataacc ttgggcaagt tgtgtagttt ctcatatact ttagttttat 120
<210> 433
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 433
ccaccttgcc tggccaaaga ttaattgtta atatacataa agcgcttaac accatgccag 60
gtaccttagt aagtgttcga tgaatatttg ctttttgtat tagccataat cattctcagg 120
<210> 434
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 434
ttcctcatga tttatataga tgtaacatgt tctataggac atgtaattta catgtcctat 60
agaactataa gttacatgtc ctatagaact tacagttcta tagttatctg cagaaatatt 120
<210> 435
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 435
acaaaccttc aatttgtgtt tttaaaaaaa tgcagtatct atgaaactca gtgaagtgaa 60
atacattaaa acaaatatac ctatgttaac tcacatatta ctgtaattaa actctgtatg 120
<210> 436
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 436
gtacatttct gggattttca gtgatgtgcc agacgagtgt ggtggtatgt tttcaactat 60
ataccgagta gaggatggga gggttctaga attttatata ttaattaaat ttggtttaaa 120
<210> 437
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 437
atttctggga ttttcagtga tgtgccagac gagtgtggtg gtatgttttc aactatatac 60
cgagtagagg atgggagggt tctagaattt tatatattaa ttaaatttgg tttaaaatgc 120
<210> 438
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 438
tgttgtttta ggatcaaata aataatctat ataaaagttc catataaatg ttagttacta 60
ttattagaac ataattttat atattaaact acctcctaaa tttttagaca ggtagatagc 120
<210> 439
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 439
ttattagaac ataattttat atattaaact acctcctaaa tttttagaca ggtagatagc 60
taaaaaaaaa ttcaaattct aagattagtt tgttagggag gaaggagcaa atattttacc 120
<210> 440
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 440
taaaaaaaaa ttcaaattct aagattagtt tgttagggag gaaggagcaa atattttacc 60
aaaactactt gtttttaatt gattaatttc attcacttga tgacttagta aatcttgtga 120
<210> 441
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 441
aaatcaggtt ttacaacaaa attgtttaaa cactgcctgc atatttagaa tctctatacc 60
tatattaaga tacagagatt ggatagtctc ccttttcagt atagataatc tcccttcccg 120
<210> 442
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 442
tatattaaga tacagagatt ggatagtctc ccttttcagt atagataatc tcccttcccg 60
gtagagatta ggatattaag atgtataata tcctaaagtg tagcagcagt ctggtatgtt 120
<210> 443
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 443
gtagagatta ggatattaag atgtataata tcctaaagtg tagcagcagt ctggtatgtt 60
acatgtctaa attccatttc ctattttatt tgtttattga tttatctgtt tatttttgtt 120
<210> 444
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 444
attaaccaca cccttaagat gagctctaat tttgttgtat ttgtcctgtt taaagccatc 60
tagttacaat agatggaact tttttgttct gattgctttt tattccaata tcttaaatgg 120
<210> 445
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 445
aattttatag aaattgtcct taattttatt ttctgcaatt tatgttttct tactatttct 60
ggtgtatgtg tttatcccat tgtgatgtta tattggtgtc ctcaatttat ttccttagcc 120
<210> 446
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 446
tgttgtgaag aattcaagaa ttttcttcta tttgttgagt tttgttttct taggagtttt 60
gctctttctc ttttgctgtg ttttctcctt attttttaaa tgtgtttgtg tttggtgagt 120
<210> 447
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 447
gctctttctc ttttgctgtg ttttctcctt attttttaaa tgtgtttgtg tttggtgagt 60
tatgttttag tgcttcgtag gtttttcttt gactatatta tattagtaag ccacattgtt 120
<210> 448
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 448
tatgttttag tgcttcgtag gtttttcttt gactatatta tattagtaag ccacattgtt 60
cccatgccat tttatttcat cttggtcata tttggatgac tcttttcaca cattttattg 120
<210> 449
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 449
atgtgctaga cagtgtttta agtgctgggt acatagcgat taacaaaaca gataagaatc 60
cctaccctca tagagcttac attatgaggt tgggggaggg agattacaaa caaagaaata 120
<210> 450
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 450
ggcaaagaat ttgggggtat atgcaaaagg aggatttaaa taattgacct tggacacaat 60
gcagagcaaa gaagagacat tagaagacgt ggatcaatga acaggagata agaaaagctg 120
<210> 451
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 451
tagagggagt gaactgggca gatcaaaggt aggtggttga agaaggaggt acttcaaatt 60
gagattctgg gggaaatgga gttattggaa ataaaagtct tgggtatgtc cattgcagtg 120
<210> 452
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 452
ttgcaacaag atggcaatga gagcacctgc aggaaagggt gacggtagtg gagatcttac 60
tgagttccag aggccccatt gaaaggattc gaggagatga agaggtagga ggagatggtg 120
<210> 453
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 453
ccagacttac ctggacaagg tagagaacat ttgcaagaag ttttcaagtc ctttctgtca 60
cagaatggaa tgtccagaga tggactgtga ggaagaacgg gccttgctgg aatgtggagg 120
<210> 454
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 454
tcacctgtat ttcatcttca atctgctgtg gaacaaaagc ccacatttga ggttgcctat 60
atagaactgg caggaaagta tatagaagca ggcaatcaca gaaaagctga agacactttt 120
<210> 455
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 455
cagcagacag aggtgggaaa tgtttgtctt ccattttgga ttccctcatg tctagttgga 60
tttgtttgtt ggttggttta ttgggtatgt gaagtatcac tgtggttcta gggagtcaga 120
<210> 456
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 456
tttgtttgtt ggttggttta ttgggtatgt gaagtatcac tgtggttcta gggagtcaga 60
cctgtacaaa aagatatact tcatgtcact tcctccttat ccttgcagtc ccaatcctaa 120
<210> 457
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 457
cctgtacaaa aagatatact tcatgtcact tcctccttat ccttgcagtc ccaatcctaa 60
tcctcctttt tcccccactc acaccctgtt cctttagttt ctgagtttat ccttcctgat 120
<210> 458
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 458
atttatctcc agaaatcttg cacaaatctg tactcctgtt agcaatgtgt gcgtatacct 60
gcttccacat gacctcagta aaagaatgtg ttgtcatatt ggtattgaaa ttttagcact 120
<210> 459
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 459
cttgcacaaa tctgtactcc tgttagcaat gtgtgcgtat acctgcttcc acatgacctc 60
agtaaaagaa tgtgttgtca tattggtatt gaaattttag cactgtaagc aacaggtcat 120
<210> 460
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 460
aataagcctc ttaattctgt agtttaatat ttgaaatgtg tgttattcag aatttatata 60
aaaatatatt ttaaaagcat tagagtagct gtataaagaa agctgtgttg acattttacc 120
<210> 461
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 461
aaaatatatt ttaaaagcat tagagtagct gtataaagaa agctgtgttg acattttacc 60
tagagactct atgcataatg aataacactc tgctatatct agtttctaaa ttaggggtgg 120
<210> 462
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 462
tagagactct atgcataatg aataacactc tgctatatct agtttctaaa ttaggggtgg 60
gagttgtatt cattatttag ttcccataca gcatatctac tgtttacacc ccacattttc 120
<210> 463
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 463
tgagatgatt cccagctatt aagtagtaaa tagagctagg acttgagccc caatcttcca 60
gcttcaatcc agatcatatg acagcttgct gattaaacta gatgacagag aagatctctt 120
<210> 464
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 464
gtgtcattaa atggaatgag gtctcttagt acagttattt tgatgcagat aattcctttt 60
agtttagcta ctattttagg ggattttttt tagaggtaac tcactatgaa atagttctcc 120
<210> 465
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 465
ttttatgaag gcaggcttat tttatttaca ctattagcac aacagccaaa agttattcag 60
gtttaatcct gctgaataaa gtagtaaaaa cacaaggcgt gactttaaat aatgacactg 120
<210> 466
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 466
tctggcatat taacattaaa ggaaaatcag tatcataaga gtcgcacatc tcacattttt 60
agatacatag attatcaaag tagcaatggc actttgataa gtagcaatgc ccccccacca 120
<210> 467
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 467
agagtcgcac atctcacatt tttagataca tagattatca aagtagcaat ggcactttga 60
taagtagcaa tgccccccca ccacttctct ccaccttccc aactttaccg atggagatga 120
<210> 468
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 468
gtcgcacatc tcacattttt agatacatag attatcaaag tagcaatggc actttgataa 60
gtagcaatgc ccccccacca cttctctcca ccttcccaac tttaccgatg gagatgaatt 120
<210> 469
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 469
cgcacatctc acatttttag atacatagat tatcaaagta gcaatggcac tttgataagt 60
agcaatgccc ccccaccact tctctccacc ttcccaactt taccgatgga gatgaatttc 120
<210> 470
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 470
gcacatctca catttttaga tacatagatt atcaaagtag caatggcact ttgataagta 60
gcaatgcccc cccaccactt ctctccacct tcccaacttt accgatggag atgaatttcc 120
<210> 471
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 471
cacatctcac atttttagat acatagatta tcaaagtagc aatggcactt tgataagtag 60
caatgccccc ccaccacttc tctccacctt cccaacttta ccgatggaga tgaatttcct 120
<210> 472
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 472
acatctcaca tttttagata catagattat caaagtagca atggcacttt gataagtagc 60
aatgcccccc caccacttct ctccaccttc ccaactttac cgatggagat gaatttcctg 120
<210> 473
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 473
catctcacat ttttagatac atagattatc aaagtagcaa tggcactttg ataagtagca 60
atgccccccc accacttctc tccaccttcc caactttacc gatggagatg aatttcctga 120
<210> 474
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 474
tctcaggacc tagctctggc aaaagcataa caaacatctt gttcagcagt tctctttcag 60
cctccactca tgtttttcat gctgccctgc acacccccaa accaccctga ggctagcaag 120
<210> 475
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 475
tcttgttcag cagttctctt tcagcctcca ctcatgtttt tcatgctgcc ctgcacaccc 60
ccaaaccacc ctgaggctag caaggagcta aagcttcaga aatgctttgt ttctggtcct 120
<210> 476
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 476
cacagttcat attccccagc caccagagtc gggaggatct ctctccagat cttgattcct 60
accagtccct taaacaattg cttacatttt cctgatcctc cagacattca ggatcagccc 120
<210> 477
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 477
ctcactattc ctgaaaaggc aggctgtact agatggatac tgcttttcct attaacccat 60
tagtgatggg gtcagaaggc tgaggcctgg cttaccctca aggcccagta gcacttctgc 120
<210> 478
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 478
ctactaacac cgagactcat caactcactg gcaggtagac agggagctgg catgggccct 60
tgggaagtca agctgtaggt gaggatgaac tggaaggctt tgacaactaa gatacacagt 120
<210> 479
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 479
taggcagaat tctaaagatg tcctcccttg attgctgtct tctggttatt cattccaaca 60
gtaatctagg tactgctgcg aagggacttt gcagctataa ttaaggctat gggccttaag 120
<210> 480
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 480
aatctaggta ctgctgcgaa gggactttgc agctataatt aaggctatgg gccttaagat 60
atgaagatca ctttggatta ccaagtgggt gcaatctaat cacttgagcc cttagaaaca 120
<210> 481
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 481
ctggttttga agatggaggc agacaggcca ggagcctagg aatgtgggga gcctcaagaa 60
gctgagaatg atgcccagcc aacagccagt gggaaacagg gatcttagtc ctagaactgc 120
<210> 482
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 482
gctgagaatg atgcccagcc aacagccagt gggaaacagg gatcttagtc ctagaactgc 60
aaggacccag agcctctaga agggaacacg ccctgcgaac acctttattt tggcctcatg 120
<210> 483
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 483
aaggacccag agcctctaga agggaacacg ccctgcgaac acctttattt tggcctcatg 60
aaacctgaaa caaagaaaca gttgagccac ctcagacttc tgaccttgca acaatttgtt 120
<210> 484
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 484
acagcacaac atttacaaaa cgtattttgt acaatcaagt cttcactgcc cttgcacact 60
gggggggcta gggaagacct agtccttcca acagctataa acagtcctgg ataatgggtt 120
<210> 485
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 485
cagcacaaca tttacaaaac gtattttgta caatcaagtc ttcactgccc ttgcacactg 60
ggggggctag ggaagaccta gtccttccaa cagctataaa cagtcctgga taatgggttt 120
<210> 486
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 486
ctttaagtca cataatcgat cccaagcact ctccttccat tgaagggtct gactctctgc 60
ctttgtgaac acagggtttt agagaagtaa acttagggaa accagctatt ctcttgaggc 120
<210> 487
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 487
gtgacagtga aagcccacag cctctggcaa ctgtattaac atgaacttca atctgttaaa 60
ggaaagccac caggaaaaca gcactgtaat ttaacgatgt ggaaaaatgt atgtaatatc 120
<210> 488
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 488
ggaaagccac caggaaaaca gcactgtaat ttaacgatgt ggaaaaatgt atgtaatatc 60
ttaaggaaaa aagcaaaaca gtgtaattat gatcacattt tataaaatac acgtgtatat 120
<210> 489
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 489
ttaaggaaaa aagcaaaaca gtgtaattat gatcacattt tataaaatac acgtgtatat 60
atacgcacat atgcctggtg gagttttatg gtgatcatct ccaagtggtg gaattactgg 120
<210> 490
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 490
ttactgtaaa tgtcaagaga tgggaagata attcatccag tcaaaaaaat acatgttatc 60
ctggttagag actcagcagg gaaaggctac atgctgagct ggaatccata tactcagggg 120
<210> 491
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 491
cttggaaatt tcataaaaat ccaattgata actaataaag aggatggaaa caaaattaca 60
ttgcagccag gttttcttgc tgtcactcat ctgcctgtga ccagatgcta aggcctttct 120
<210> 492
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 492
ttgcagccag gttttcttgc tgtcactcat ctgcctgtga ccagatgcta aggcctttct 60
ctaaggtgta agaggaccta agtccctggg cagaagcagg cacaggaggc aaaggtgggt 120
<210> 493
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 493
ctaaggtgta agaggaccta agtccctggg cagaagcagg cacaggaggc aaaggtgggt 60
agcttttgct gtgaaaagaa caaaccaaat ttatacacac accaaggcct ttgtgctgcg 120
<210> 494
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 494
aaaaaaagaa aaaaaaaaag aaatgttcac cgagaatctt cccctgctct gggcccgtcc 60
gtggtgggcc agctgctgtg ctttcttcta tgtaagtaaa ttaagatggt ttaggaagag 120
<210> 495
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 495
cacctgatga tttctgctgc tacttcccag ggacaagcag tccaatgtcc agaacactac 60
tggatttcag aagatcttct tgaagtgcat atgtagttga cctgcactct acaggcattc 120
<210> 496
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 496
aactcaaagt tattggctga agtttgatgt ttatccagac ttggtacctc aagtactcac 60
tatgacccca tcaacagagg ggtctatgtt gattttaggt gtacatgctc cttgtctcct 120
<210> 497
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 497
cattgtatct ttaggccacc taagcagttt ttaatctaga agtgaagcca ggttctgcct 60
ctcctcattg aactagtctg actacctggt ttcctgaaga cacagatcac actgcactgc 120
<210> 498
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 498
ctcctcattg aactagtctg actacctggt ttcctgaaga cacagatcac actgcactgc 60
ctcttccact tacagggctt ggcaacactg ttcaaaacaa ctttctgtga tagtcacaat 120
<210> 499
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 499
ctcttccact tacagggctt ggcaacactg ttcaaaacaa ctttctgtga tagtcacaat 60
gttctatatc tatgctaata aggtagccat gagtcacata tagttattga gcactggaga 120
<210> 500
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 500
agacagacgg acagaaacac acacacacac acacacacac acacacacac acacacacac 60
tctcttactt taccgccaga gtgaaaagaa atggcagtag gacaagtctg tgtgtttttt 120
<210> 501
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 501
aaaaaagaaa tttgaagttt cacctctaca tatacatttc tcttccagag aaaaatacta 60
tggtgacatt tagtattttc agaatgtcat tactttgacc acatactttc catcattgct 120
<210> 502
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 502
aatttagact tggagctaga cttcctgagt tcaacttcta gttccatcac ttattacctg 60
tgtgactttg agcaacttat tcaacctctc tgagcctcaa ttttcacatc tgtaatatag 120
<210> 503
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 503
tgtgactttg agcaacttat tcaacctctc tgagcctcaa ttttcacatc tgtaatatag 60
gcataattac agtacatagc tcatagagtt gtcataaaat tgttaattag ttaaaacatg 120
<210> 504
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 504
gcataattac agtacatagc tcatagagtt gtcataaaat tgttaattag ttaaaacatg 60
gttgggcctt tagagcagtc ccagcataaa gtgatctttt tttttttttt ttcttttgag 120
<210> 505
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 505
aattgctggg cagagatttg accaaaagca agtaaaatca ttctttttgg tagaccaggt 60
gaaatgacta ggatctcagc tgttgaaatc aattcactga agcaaatctt ttgtgtgcag 120
<210> 506
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 506
gaaatgacta ggatctcagc tgttgaaatc aattcactga agcaaatctt ttgtgtgcag 60
aacttaacaa tcggcttttc acctcactgg gacttaatgt attttaattt tcaacatcta 120
<210> 507
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 507
aacttaacaa tcggcttttc acctcactgg gacttaatgt attttaattt tcaacatcta 60
tctcctctca aaacattcac ttagcattta gattagactg ctaatactgt gctgttataa 120
<210> 508
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 508
ccatattccc taccatgcct tcttcatcta atttaatatt actaacaaga tctattgttc 60
aagccaaaac ccaagtgtca ctccttcaat ttctctttac cttatcctcc aaatttaatc 120
<210> 509
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 509
aatcaacaac ctttgacagc taatactatt ccactgttct gtatatttct ccatagcatt 60
tattgttatc ttaaattcat ctttattgtg tatctcccct cgacagaacc tgaatcctac 120
<210> 510
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 510
tattgttatc ttaaattcat ctttattgtg tatctcccct cgacagaacc tgaatcctac 60
cagggactta gttagtctta tttactgttg cattcctagt gcccagaaca cagtaggctc 120
<210> 511
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 511
cagggactta gttagtctta tttactgttg cattcctagt gcccagaaca cagtaggctc 60
ccaataaata gccactgaat aaaagttaaa accaacaaaa ataatcattt aattaattat 120
<210> 512
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 512
ctgttgtcac agaagttctc agatggctgt tctgaaatta ttcctcctcc tacactctat 60
cttatttact tctcactgtt ctcagtatca taaagtgcaa catctttttg aagcaatctg 120
<210> 513
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 513
tacaattaaa gaccttttgg taactcagac tcagcatcag caaaaacctt aggtgttaaa 60
cgttaggtgt aaaaatgcaa ttctgaggtg ttaaagggag gaggggagaa atagtattat 120
<210> 514
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 514
gtatggatat ttggaagtct aggggaggct gatatgaagt taagaccaga ggaaatatct 60
gtccactccc tcttctcaac acccatcttc tagacgccaa ggctagctat agatctccat 120
<210> 515
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 515
tatggatatt tggaagtcta ggggaggctg atatgaagtt aagaccagag gaaatatctg 60
tccactccct cttctcaaca cccatcttct agacgccaag gctagctata gatctccatt 120
<210> 516
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 516
gaatcacttg tttatttccc agtgtttcaa aggcccttct gaagaactaa gcaaactaag 60
gaaagaccat ttagttttaa acaggagaaa tgtatttaac taaatcctaa acacagcagg 120
<210> 517
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 517
gaaagaccat ttagttttaa acaggagaaa tgtatttaac taaatcctaa acacagcagg 60
ctatctgcaa gcagcagcag cagcagcagc catgctccct cacagaatcc ttacaatttt 120
<210> 518
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 518
ctatctgcaa gcagcagcag cagcagcagc catgctccct cacagaatcc ttacaatttt 60
tgaagttttt tgtttaactg ctacaaaagc cgatttagta acatttatta cacttaaaaa 120
<210> 519
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 519
gtctgctttc tggagcccca tgagtgagtc ttaatcctgt tccagataac agttctcttc 60
cgggtaacgg ttcttcagat acttgaagac agtgtcttat ttccttaaat cttctcattt 120
<210> 520
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 520
ccagtttcaa ataagcattt ttagcagccc tgaaatgtgt actcagattt agtttatagt 60
caactaaaaa cacccagagg tctcctgtat tacacaagtt ataattaaaa ccttaaaaga 120
<210> 521
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 521
caactaaaaa cacccagagg tctcctgtat tacacaagtt ataattaaaa ccttaaaaga 60
gaaaggtata ggacaaatga tctgtctcct cccttttttg ctttttcata tgttaagact 120
<210> 522
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 522
gaaaggtata ggacaaatga tctgtctcct cccttttttg ctttttcata tgttaagact 60
atctcggagc tgttatcaga cttttttcct gaaaaactct caacaatact caaactaggt 120
<210> 523
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 523
gtataggaca aatgatctgt ctcctccctt ttttgctttt tcatatgtta agactatctc 60
ggagctgtta tcagactttt ttcctgaaaa actctcaaca atactcaaac taggtgttac 120
<210> 524
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 524
aaatatatat taattttgct ttaatagcag atatcattcg attccctaag atcgtttctt 60
tgcatagtga cactctactg tactataaat ttatcagcct acactttata agctgagagg 120
<210> 525
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 525
tgcatagtga cactctactg tactataaat ttatcagcct acactttata agctgagagg 60
taactagcct acacagaaag ttatgctaca gatatacatg gcttaaaacc aagactcaac 120
<210> 526
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 526
taactagcct acacagaaag ttatgctaca gatatacatg gcttaaaacc aagactcaac 60
cctctgatag ttacaaaaca ctgaaatcag gatgttacta aaggtcagtg caaaagcgat 120
<210> 527
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 527
gccatcactg tgcacttact cattgtatct accagggcag ccacagctta ctttgccctt 60
ttagtagtgg gatacatctg gccacagaga tgttatgcta agtaactacc tatggagaag 120
<210> 528
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 528
ttagtagtgg gatacatctg gccacagaga tgttatgcta agtaactacc tatggagaag 60
ttaggatgaa aaatcctagg aaggatatat taataaagat atttcagaat gacttggtca 120
<210> 529
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 529
ttaggatgaa aaatcctagg aaggatatat taataaagat atttcagaat gacttggtca 60
tctctcagtg tgcaataaat ggcacaccca ctactgaatc catttgataa tctggtatgt 120
<210> 530
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 530
caattcccat ttctggcatt aaggacccaa ggtgatgcca aaatcctaat agcaagggat 60
attgcagggc agaagtggca gggcattctt acaagccagg atgaaaacaa acactagaga 120
<210> 531
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 531
ggaaaattcc aattcgaaag tcctatatca tacccaaagt atagcttttc cactaatatt 60
taataattat tttctcaagt aaatatacat aaaataatcc acactatagc tttatctaga 120
<210> 532
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 532
tcacactttc ttccattgca ttatacccag cagtatcagt agtatgagca gcagctggac 60
tctgggcaga ttctgcaact ttcaattggg gaactttcaa tgcagaggtt gaagatggta 120
<210> 533
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 533
tatagcaatt ttgtaagaaa ggaaaaaaaa gttgaaaaat gtggcaaatg ctttattttt 60
gtttcttcat ccaaggtaat gacaccatta cttttttcac ttaataaatc agcagatatc 120
<210> 534
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 534
gtttcttcat ccaaggtaat gacaccatta cttttttcac ttaataaatc agcagatatc 60
attaccaaat taaaaacagc agagatgtca tccttgaaac atttcagaaa atgccatggt 120
<210> 535
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 535
attaccaaat taaaaacagc agagatgtca tccttgaaac atttcagaaa atgccatggt 60
atcaggaatt aagcatagct aagtagaatg tatgagattc ctaaaatgac cctaatgaac 120
<210> 536
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 536
agaaagataa atggaaggag aaaaccatcg ccaccaattg tgaaaggaca aatcatactt 60
gctgggcagc caaagcataa atgaaacagc tcatgtcaga gagatcagaa atgactggca 120
<210> 537
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 537
gcataaatga aacagctcat gtcagagaga tcagaaatga ctggcaaaaa agagcccgca 60
agacagccta gaagtctgga ttcatgttgc ctgccaatat gtcagggttg acatataaca 120
<210> 538
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 538
ccatgcctgg ccaaggcgga aatatttaat aagtaaaaac aaatagttaa aaattgcaaa 60
agtcttctat tcattaaaaa gtcactgatg actgtaattc attttgagct tttattagaa 120
<210> 539
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 539
tgatgactgt aattcatttt gagcttttat tagaaaagct aaaagccata aatataggta 60
gaaaattgaa acaaaagatt gtattaatta aactgttttt ttaagacact gggtagcctg 120
<210> 540
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 540
gaaaattgaa acaaaagatt gtattaatta aactgttttt ttaagacact gggtagcctg 60
gggggaaata atcattgatt tcctgagtta gtcttcaaat gcattattta tagtttcctt 120
<210> 541
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 541
gggggaaata atcattgatt tcctgagtta gtcttcaaat gcattattta tagtttcctt 60
tatgcttgtc ttttctggac ctgtgagttt ctaggattgt taagaactct gttgaaagta 120
<210> 542
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 542
aaagaaaagt cattctatca ccagaacatt tagcatataa attcctcttc ttactacaat 60
gggcctcatg caatgaagca aataagataa cttgttagaa gttaacaact caatagaacc 120
<210> 543
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 543
gtggaacggg gtgctgaaga aggaacttta acactcagat aagaatcact gagctaggaa 60
tgaagtgtac tttgggagta ctcaccaccc ctgcatgttt ctaagcagca tcctagtggg 120
<210> 544
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 544
tgaagtgtac tttgggagta ctcaccaccc ctgcatgttt ctaagcagca tcctagtggg 60
gaccccatgc ctagcatctc ctatgtgtct gtttgtttgc ccctatttaa aaatctgcct 120
<210> 545
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 545
gaccccatgc ctagcatctc ctatgtgtct gtttgtttgc ccctatttaa aaatctgcct 60
gatgtattgt ctttccaatt aaaaactcaa atttccatct cactttcctt catttatgcc 120
<210> 546
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 546
acaatacaca cctttttctg atgtgctttg ttctggattt cgcaggtcct caagggcaga 60
agagtcactt atgatggaag ggtagctgtt agaaggctgg ctcccatgct gttctaacac 120
<210> 547
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 547
acctttagtt tctaacagaa aaagcaaaaa gctaattcat gaatctatct gtttgctcag 60
ttaattagac aactatttac tatctattaa gcaccaggcg ctgtcctagt actgaggata 120
<210> 548
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 548
ttaattagac aactatttac tatctattaa gcaccaggcg ctgtcctagt actgaggata 60
tagccctcat ggtgctgcca ttctagacag ggacatctca tctagaatgt gtccacctag 120
<210> 549
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 549
tagccctcat ggtgctgcca ttctagacag ggacatctca tctagaatgt gtccacctag 60
agatcattag caaggacctg tgagagggaa ggatccaaca gaaagagccc tgagaaagcc 120
<210> 550
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 550
aaataatgag ttctttttgc ttatgggctc ctgttgttta ttggtccatt tcaaagaaga 60
gtgtgctaag tccaagtatt tgataaacaa agaatttagg tatgtaagga gttttccaaa 120
<210> 551
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 551
actccatctc aaaaaaaaaa attaggagaa agagcctggt tctatgatga agatgatatt 60
catttttaga cgttgtgata cagaatgttt tttctagact attctgcctt tagtacataa 120
<210> 552
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 552
tacataaaca tttcatatta cattatcaaa tggtcatata tatggtaaag cttttattta 60
tttcataggt gaccaaatta ttggccttga ccattaggtc cagaaagtaa aattgtgtac 120
<210> 553
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 553
aagaagaaga gaaagaagta tccacaaaac agtgaaaggc tagatggtct ctattaggaa 60
gagaacccta tagtctccaa cttggtacca agaataaaca ccaaaattgc gattgctggc 120
<210> 554
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 554
gagaacccta tagtctccaa cttggtacca agaataaaca ccaaaattgc gattgctggc 60
aataatattg gtgagagttc tgtacctggc tgtgtggcct taggcagttt gcttaccttc 120
<210> 555
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 555
aataatattg gtgagagttc tgtacctggc tgtgtggcct taggcagttt gcttaccttc 60
tctgacctgc agtaggcata ttcttaaaat ggagctatgc agagtccctt gaatcatcct 120
<210> 556
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 556
ctgtgtggcc ttaggcagtt tgcttacctt ctctgacctg cagtaggcat attcttaaaa 60
tggagctatg cagagtccct tgaatcatcc tgctgtggct cctttttctc acctctcttg 120
<210> 557
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 557
gcaggactgg atttactttc atgtcacaca aaatgattaa attccttgct ttgggacacc 60
tggatttgct tttataaaat gaaaccagaa gtaagtccac cagtaattag gatgttaaag 120
<210> 558
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 558
cggtaaccct gagccaaatg tgtatgggtg aaagggctag gactcctgct aagctctcct 60
ttctggacgc ttttgctaaa aacagcagaa ctttccttaa tgtcattttc agcaaaacta 120
<210> 559
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 559
atactggagc ccacttcatt agtactggaa cctacttcat taatattgct tgagctggct 60
tctttaaaaa cattttctct aatgttatta cggctaattg tgctcactgt acttggaatg 120
<210> 560
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 560
gacccagagt gggcagagaa tgttgcacat tcctcttctg catttcctgg atttgaaaac 60
ggagcaaatg actggcgctt tgaaaccttg aatgtattct gcaaatactg agcatcaagt 120
<210> 561
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 561
gagtgctaac ttccagtaac gagatacttt cctgagtgcc ataatcagta ccaggtacca 60
atgaaatact gctactctct acagatcttt cagtttgcaa aaccctttct ccacttaaca 120
<210> 562
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 562
atttgaacac ttagtaaaag aaccaggtgc atttgttaac ttcagctctg ggaaagtatc 60
gctgtcatgt cttttacttg tctgttcatt tggcttgtta ctcttcttgg ctccagttgc 120
<210> 563
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 563
taattcctaa ggaaggtagg taacatagtt ggggttgctt gtagtttatt atataacata 60
cagacaaagc ttgagagata tgtggaaata aggaacagag gaaagaaaga gttgaaaatg 120
<210> 564
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 564
cagacaaagc ttgagagata tgtggaaata aggaacagag gaaagaaaga gttgaaaatg 60
aagtccttga acattttcat cagtcacaat tactgctgtg tcaatattac tgtatatgag 120
<210> 565
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 565
aagtccttga acattttcat cagtcacaat tactgctgtg tcaatattac tgtatatgag 60
gaacttagag aacaatctgc tagaagcaag catgtgcaga gttctactgt tatcctaaat 120
<210> 566
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 566
cctcttttgc tcccttttta aagtaagatt cttcaaggtg ggaactgcgt cttttacatt 60
ttttataact caccataggg ctcataaaat tcacttccca aagctgccta ccacaaatac 120
<210> 567
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 567
cagcaacagt agaaaacctc tataatcaat acatcattga catctgtata aaccgtgtga 60
tggcagtgat ttagtaactt tttgtcattc atttaagcct accaaatgcc taaaattatc 120
<210> 568
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 568
tcaaggattt ctgtcaaatt atgattatac ttctttgaaa acacatagat aacaatgtcc 60
aaagggagat ttgggtttag taaagtggaa ttaaagcaca attcattcat tcctttaagc 120
<210> 569
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 569
aaagggagat ttgggtttag taaagtggaa ttaaagcaca attcattcat tcctttaagc 60
atctgagtgc ctactatgta ccaaacttag gaaaaacagt gttgagacag acaaaattac 120
<210> 570
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 570
atctgagtgc ctactatgta ccaaacttag gaaaaacagt gttgagacag acaaaattac 60
tactctcata gaatttacat tgtagcagaa gagatacaca atatataaat acattgtgtt 120
<210> 571
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 571
tgtttttaac aaacagtgtt gagacaaaag tataaccaca tgtaaaagaa tgaaattata 60
ctcctatctc acaccaaata acaaaagtta actcaaagtg gatcatagac cagaagtaga 120
<210> 572
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 572
ctcctatctc acaccaaata acaaaagtta actcaaagtg gatcatagac cagaagtaga 60
gagagcaatg gtgatgataa ttaaaagcag cgattcactc atacaaccaa tatttactat 120
<210> 573
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 573
gagagcaatg gtgatgataa ttaaaagcag cgattcactc atacaaccaa tatttactat 60
gtctagtact attctagatg gaggggattc aatagaaaag caaaataaga tcctgcaaaa 120
<210> 574
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 574
atttatatgt atacacacac atatatcttt atgactggtt ctttcctgac tatcacaatc 60
ctgactatca caagcttgaa accaagcttc tcactcttct ccagttgcca actctaatcc 120
<210> 575
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 575
atacacacac atatatcttt atgactggtt ctttcctgac tatcacaatc ctgactatca 60
caagcttgaa accaagcttc tcactcttct ccagttgcca actctaatcc tctcaatctc 120
<210> 576
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 576
tacacacaca tatatcttta tgactggttc tttcctgact atcacaatcc tgactatcac 60
aagcttgaaa ccaagcttct cactcttctc cagttgccaa ctctaatcct ctcaatctca 120
<210> 577
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 577
acacacatat atctttatga ctggttcttt cctgactatc acaatcctga ctatcacaag 60
cttgaaacca agcttctcac tcttctccag ttgccaactc taatcctctc aatctcaaca 120
<210> 578
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 578
ttattatctc tgcctataag cctataattt ccttcttcca caagttctca aagcaactat 60
attcataact taatctctct ttttttgtta agagacaggg tcagccaggc gcggtggctt 120
<210> 579
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 579
acaaattagt tgacatttaa ggtaagatgt gagccatagc ttaacataaa gaaaaggaga 60
aagcccactt gttctactaa attacctact ctactttttc tgaaattctt ttattaagaa 120
<210> 580
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 580
aagcccactt gttctactaa attacctact ctactttttc tgaaattctt ttattaagaa 60
acaaacattt tcatttcaaa agacaagatc atcaaatacg taaatataac ttgaatcact 120
<210> 581
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 581
acaaacattt tcatttcaaa agacaagatc atcaaatacg taaatataac ttgaatcact 60
gctataaatg aattttggcc taaatagaaa ctggtatcag gtcctttcct gtcttcacaa 120
<210> 582
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 582
ttttggccta aatagaaact ggtatcaggt cctttcctgt cttcacaatg attacaaagc 60
gggcaaacac tgacccttag aagggggaat gcataaggat atgcagaaat gaacagaaag 120
<210> 583
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 583
atctttaagc tccataaaga caaaattttc tgcttatatt tcttttgaat tccttcctag 60
aacttttttt tgtttactgt aaatgccttt ttaacttaat atggaaaatt tcaagtattt 120
<210> 584
<211> 120
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 584
ctgcccccct ccccagggtt cacaacgcct tacgcctctc aggttccgcc cctacccccc 60
gtcaaagaat acccatctgt cagcttcgga aatccactct cccacgccag taccccagag 120
Claims (7)
1.一组捕获探针，适用于大片段重排检测，其特征在于，包括针对基因每一个外显子及所述外显子两端60bp区域的探针，和针对单核苷酸多态性位点的探针；
所述针对单核苷酸多态性位点为基因内含子区域以及基因的上下游各5000bp范围内的SNP位点，所述SNP位点的人群发生频率介于30％-70％；
所述基因为BRCA1/2；
所述捕获探针为SEQ ID NO:1～584所示的探针的混合物；
所述捕获探针能够对每一个外显子及外显子两端60bp区域2X全覆盖，所述外显子及其两端60bp的区域至少有3条不同探针覆盖；
所述捕获探针还覆盖所有外显子区域的SNP位点。
2.一种基于下一代测序平台检测大片段重排的试剂盒，其特征在于，包括权利要求1所述的捕获探针。
3.一种权利要求1所述捕获探针在制备按照如下方法检测大片段重排的试剂盒中的应用，其特征在于，所述方法包括以下步骤：
(1)对目标样本进行DNA抽提；
(2)制备测序文库；
(3)测序，并进行生信流程分析。
4.如权利要求3所述的应用，其特征在于，所述步骤(2)包括以下步骤：
(a)将DNA片段化，然后再对片段的末端进行修复、磷酸化，之后在片段的3’端加脱氧腺嘌呤，再连接接头；
(b)进行扩增反应，扩增后的产物与权利要求1所述的捕获探针进行杂交，杂交后对片段进行特异性洗脱，再进行PCR扩增，得到测序文库。
5.如权利要求3所述的应用，其特征在于，所述生信流程分析的算法是由序列深度算法模块、等位基因不平衡算法模块和非一致序列算法模块整合而成的。
6.如权利要求5所述的应用，其特征在于，先综合序列深度算法模块和非一致序列算法模块对结果进行处理，确认潜在的大片段重排。
7.如权利要求6所述的应用，其特征在于，对潜在的大片段重排应用等位基因不平衡算法模块处理，以排除假阳性，确定真实的大片段重排。
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| CN114606303A (zh) * | 2022-05-09 | 2022-06-10 | 北京普康瑞仁医学检验所有限公司 | 一种用于检测brca基因大片段重排的探针、试剂盒及应用 |
| CN116179710B (zh) * | 2023-03-14 | 2023-11-24 | 成都诺森医学检验有限公司 | 基于高通量测序平台的brca1/2基因突变检测的引物组、文库构建与环化方法 |
| CN117265069B (zh) * | 2023-09-21 | 2024-05-14 | 北京安智因生物技术有限公司 | 基于半导体测序平台检测brca1/2基因拷贝数变异 |
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