CN110541025B - 杜氏肌营养不良基因缺陷的检测方法、引物组合物及试剂盒 - Google Patents

Abstract

本发明涉及一种杜氏肌营养不良基因缺陷的检测方法、引物组合物及试剂盒，针对杜氏肌营养不良基因缺陷，在二代测序平台建立了一种通用性强、诊断率高、成本低的胚胎植入前遗传学检测的方法。本发明针对杜氏肌营养不良基因编码区位点和目标SNP位点对受检样本进行多重PCR并进行建库，然后对多重PCR产物进行高通量测序分析，结合家系信息构建突变等位基因的单体型，从而判断胚胎基因型，最终确定子代的杜氏肌营养不良基因缺陷检测结果。该方案省去了细胞水平预实验，基于高通量测序技术，可通过在每个样品上加不同的标签序列，从而一次性对大量样品进行分析，平均测序深度可达100X以上。

Description

杜氏肌营养不良基因缺陷的检测方法、引物组合物及试剂盒

技术领域

本发明涉及基因检测领域，特别是涉及杜氏肌营养不良基因缺陷的检测方法、引物组合物及试剂盒。

背景技术

杜氏肌营养不良(DMD)是一种常见的X连锁隐性遗传的致死性神经肌肉疾病，其发病率约1/3500活产男婴，无明显地理或种族差异。患者通常为男性，起病常在幼年。主要临床表现为全身骨骼肌进行性无力、萎缩和小腿腓肠肌假性肥大，走路姿势异常，容易跌倒，爬楼、蹲起费力，病情缓慢进行性加重，逐渐卧床，多于20岁左右因呼吸衰竭和/或心功能不全而死亡。DMD致病基因是迄今为止发现的人类最大基因，全长约2.4Mb，约占X染色体的1.5％和整个人类DNA序列的0.1％。DMD基因共有79个外显子，78个内含子，其中内含子部分占基因全长的99％。不仅基因巨大，DMD基因的突变类型也多种多样，包括多种致病突变类型，其中大片段(大于或等于1个外显子)缺失突变约占60％，大片段重复、微小缺失或插入占7％，单核苷酸点突变占20％，剪切位点突变占1％，还有少量复杂突变和内含子突变。目前DMD尚无有效的治疗方法，对女性携带者进行产期诊断或胚胎植入前遗传学检测(Preimplantation genetic testing，PGT)，是预防DMD出生缺陷的有效手段。PGT是指当胚胎在体外发育至卵裂期或囊胚期时，活检若干细胞进行遗传学检测，最终选择无患病风险的胚胎植入母体子宫，从而达到生育健康后代的目的。PGT可有效避免因妊娠遗传病胎儿而终止妊娠所带来的身心伤害，越来越成为有生育遗传病高风险胎儿夫妇的首选。在胚胎水平进行DMD基因检测的常见方法有多重巢式PCR及Karyomapping技术。多重巢式PCR法需要预先为DMD女性携带者筛选出杂合的短串联重复序列(STR)，然后在淋巴细胞水平建立包含突变位点和杂合STR位点的多重PCR体系，并在细胞水平评估各位点扩增效率及等位基因脱扣率，合格后才能应用于胚胎检测。由于STR数量有限，且人群中杂合频率不同，所以多重巢式PCR法设计较为个性化，通用性较低，个性化强也意味着工作量大，周期长。Karyomapping技术即通过SNP连锁分析间接排除基因缺陷，该芯片上有30万个SNP探针用于全基因组的连锁分析，是一种综合的通用的单基因病PGD技术。但是Karyomapping不能检测基因突变，所以必须要有先证者样本或合适的家系成员样本，如果没有足够的家系样本，则需要额外增加突变检测。虽然DMD致病基因存在一些突变相对高发的区域，但所有外显子均报道有突变，并且30％的患者的突变为新发，DMD基因长达2.4Mb，且基因内部的重组概率约为12％。

发明内容

基于此，有必要提供一种通用性强、诊断率高的杜氏肌营养不良基因缺陷的检测方法。

一种杜氏肌营养不良基因缺陷的检测方法，包括以下步骤：

获取子代的DNA样本和亲代双方的DNA样本；

针对杜氏肌营养不良基因编码区位点和目标SNP位点分别设计合成多个引物对，所述目标SNP位点包括杜氏肌营养不良致病基因内含子区的SNP位点、杜氏肌营养不良致病基因的上下游区域SNP位点和Y染色体的SNP位点，所述杜氏肌营养不良致病基因的上下游区域SNP位点根据以下原则进行筛选得到：SNP位点位于杜氏肌营养不良致病基因上游6Mb及下游4Mb以内且收录于千人基因组计划数据库，同时SNP位点的最小等位基因频率大于0.2，然后去除多聚核苷酸和上下游50bp序列中GC含量大于70％的SNP位点，去除上下游50bp序列在人类基因组中具有同源性的SNP位点；

使用所述多个引物对对所述子代的DNA样本和所述亲代双方的DNA样本进行多重PCR并进行建库，然后进行测序分析；

对所述测序分析得到的数据进行处理，得到所述亲代双方和所述子代的基因型和单体型，从而确定所述子代的杜氏肌营养不良基因缺陷检测结果。

本发明针对杜氏肌营养不良基因缺陷，在二代测序平台建立了一种通用性强、诊断率高、成本低的胚胎植入前遗传学检测的方法。本发明针对杜氏肌营养不良基因编码区位点和目标SNP位点对受检样本进行多重PCR并进行建库，然后对多重PCR产物进行高通量测序分析，结合家系信息构建突变等位基因的单体型，从而判断胚胎基因型，最终确定子代的杜氏肌营养不良基因缺陷检测结果。该方案省去了细胞水平预实验，基于高通量测序技术，可通过在每个样品上加不同的标签序列，从而一次性对大量样品进行分析，平均测序深度可达100X以上。

具体实施方式

为了便于理解本发明，下面将对本发明进行更全面的描述，并给出了本发明的较佳实施例。但是，本发明可以以许多不同的形式来实现，并不限于本文所描述的实施例。相反地，提供这些实施例的目的是使对本发明的公开内容的理解更加透彻全面。

除非另有定义，本文所使用的所有的技术和科学术语与属于本发明的技术领域的技术人员通常理解的含义相同。本文中在本发明的说明书中所使用的术语只是为了描述具体的实施例的目的，不是旨在于限制本发明。本文所使用的术语“和/或”包括一个或多个相关的所列项目的任意的和所有的组合。

在本发明中，读段(reads)是指测序获得的序列片段；单核苷酸多态性(singlenucleotide polymorphism，SNP)主要是指在基因组水平上由单个核苷酸的变异所引起的DNA序列多态性；单体型(Haplotype)指位于一条染色体特定区域的一组相互关联，并倾向于以整体遗传给后代的单核苷酸多态的组合，又称单倍体型或单元型；测序深度即例如在一个实施方案中，测序深度为1000x，则代表该条特异PCR扩增产物被测序1000次。

本发明一实施例的杜氏肌营养不良基因缺陷的检测方法，包括以下步骤S1～S4：

S1、获取子代的DNA样本和亲代双方的DNA样本。

S2、针对杜氏肌营养不良基因编码区位点和目标SNP位点分别设计合成多个引物对，目标SNP位点包括杜氏肌营养不良致病基因内含子区的SNP位点、杜氏肌营养不良致病基因的上下游区域SNP位点和Y染色体的SNP位点。其中，杜氏肌营养不良致病基因的上下游区域SNP位点根据以下原则进行筛选得到：SNP位点位于杜氏肌营养不良致病基因上游6Mb及下游4Mb以内且收录于千人基因组计划数据库(http://www.ncbi.nlm.nih.gov/variation/tools/1000genomes/)，同时SNP位点的最小等位基因频率大于0.2，然后去除多聚核苷酸和上下游50bp序列中GC含量大于70％的SNP位点，去除上下游50bp序列在人类基因组中具有同源性的SNP位点(即上下游50bp序列比对到人类基因组有多个位置的SNP位点)。

S3、使用上述多个引物对对子代的DNA样本和亲代双方的DNA样本进行多重PCR并进行建库，然后进行测序分析。

S4、对测序分析得到的数据进行处理，得到亲代双方和子代的基因型和单体型，从而确定子代的杜氏肌营养不良基因缺陷检测结果。

本发明针对杜氏肌营养不良基因缺陷，在二代测序平台建立了一种通用性强、诊断率高、成本低的胚胎植入前遗传学检测的方法。本发明针对杜氏肌营养不良基因编码区位点和目标SNP位点对受检样本进行多重PCR并进行建库，然后对多重PCR产物进行高通量测序分析，结合家系信息构建突变等位基因的单体型，从而判断胚胎基因型，最终确定子代的杜氏肌营养不良基因缺陷检测结果。该方案省去了细胞水平预实验，基于高通量测序技术，可通过在每个样品上加不同的标签序列，从而一次性对大量样品进行分析，平均测序深度可达100X以上。可以理解，该检测方法是针对受精卵在体外发育至卵裂期或囊胚期时取若干细胞进行遗传学检测，不是以有生命的人体或者动物体为直接实施对象，不属于疾病的诊断和治疗方法。

在一个具体示例中，杜氏肌营养不良基因编码区位点和目标SNP位点包括chrX:30236878、chrX:30241692、chrX:30259905、chrX:30261002、chrX:30271693、chrX:30317913、chrX:30326983、chrX:30597320、chrX:30636585、chrX:30647244、chrX:30691738、chrX:30749081、chrX:30753378、chrX:30849176、chrX:30889857、chrX:30917186、chrX:30948257、chrX:30993883、chrX:31084491、chrX:31088893、chrX:31092450、chrX:31111926、chrX:31115833、chrX:31139915-31140089、chrX:31141178、chrX:31144635-31144908、chrX:31149804、chrX:31152120-31152394、chrX:31164356-31164630、chrX:31165253-31165461、chrX:31165403-31165643、chrX:31165586-31165754、chrX:31183595、chrX:31187324-31187598、chrX:31187531-31187802、chrX:31189180、chrX:31190371-31190631、chrX:31191526-31191800、chrX:31193037、chrX:31195849-31196122、chrX:31196718-31196992、chrX:31198204、chrX:31198387-31198661、chrX:31200629-31200901、chrX:31200838-31201066、chrX:31207044、chrX:31221996-31222270、chrX:31223632、chrX:31224579-31224851、chrX:31227386-31227660、chrX:31227599-31227864、chrX:31234101、chrX:31237433、chrX:31240800、chrX:31241076-31241334、chrX:31245191、chrX:31251156、chrX:31259670、chrX:31266919、chrX:31278012、chrX:31278988-31279233、chrX:31284828-31284997、chrX:31289621、chrX:31309895、chrX:31320374、chrX:31325686、chrX:31336718、chrX:31340552、chrX:31341618-31341887、chrX:31344293、chrX:31348807、chrX:31359018、chrX:31366534-31366785、chrX:31376888、chrX:31383766、chrX:31393051、chrX:31407830、chrX:31415050、chrX:31421134、chrX:31429432、chrX:31438422、chrX:31462561-31462813、chrX:31473656、chrX:31482430、chrX:31491980、chrX:31496147-31496420、chrX:31496359-31496521、chrX:31497010-31497284、chrX:31500990、chrX:31506013、chrX:31510603、chrX:31514846-31515088、chrX:31523257、chrX:31525350-31525623、chrX:31526203-31526476、chrX:31534414、chrX:31546065、chrX:31554545、chrX:31565405、chrX:31574895、chrX:31582896、chrX:31589214、chrX:31596103、chrX:31604296、chrX:31608475、chrX:31616684、chrX:31620838、chrX:31632103、chrX:31637820、chrX:31645554-31645823、chrX:31645765-31646032、chrX:31646613、chrX:31654664、chrX:31661326、chrX:31666315、chrX:31669448、chrX:31675908-31676168、chrX:31676101-31676348、chrX:31684749、chrX:31696019、chrX:31697352-31697549、chrX:31697486-31697757、chrX:31712956、chrX:31718900、chrX:31725847、chrX:31730752、chrX:31747688-31747926、chrX:31752869、chrX:31764087、chrX:31772239、chrX:31778126、chrX:31787455、chrX:31791926-31792177、chrX:31792118-31792382、chrX:31792559、chrX:31805056、chrX:31820514、chrX:31835517、chrX:31838008-31838282、chrX:31843791、chrX:31848300、chrX:31854753-31855027、chrX:31863313、chrX:31867663、chrX:31873388、chrX:31879756、chrX:31887778、chrX:31893104-31893376、chrX:31893312-31893541、chrX:31896669、chrX:31901450、chrX:31905515、chrX:31921060、chrX:31927171、chrX:31947659-31947919、chrX:31948624、chrX:31950011-31950250、chrX:31950188-31950418、chrX:31952826、chrX:31967612、chrX:31971174、chrX:31983162、chrX:31986312-31986556、chrX:31986498-31986666、chrX:31993673、chrX:32014860、chrX:32020257、chrX:32077969、chrX:32083312、chrX:32087054、chrX:32090183、chrX:32096769、chrX:32112345、chrX:32126093、chrX:32234839-32235113、chrX:32235050-32235324、chrX:32305456-32305700、chrX:32305645-32305918、chrX:32328028-32328242、chrX:32328175-32328449、chrX:32360186-32360454、chrX:32361107-32361332、chrX:32361274-32361464、chrX:32363864-32364128、chrX:32364059-32364233、chrX:32366479-32366742、chrX:32380847-32381114、chrX:32382616-32382876、chrX:32383091-32383358、chrX:32398586-32398770、chrX:32398710-32398983、chrX:32404306-32404537、chrX:32404476-32404748、chrX:32407590-32407830、chrX:32408099-32408373、chrX:32429833-32430096、chrX:32430022-32430296、chrX:32430166-32430417、chrX:32456199-32456417、chrX:32456354-32456580、chrX:32459112-32459386、chrX:32459327-32459566、chrX:32466397-32466671、chrX:32466613-32466858、chrX:32472657-32472856、chrX:32472788-32472991、chrX:32481435-32481612、chrX:32481548-32481806、chrX:32482623-32482870、chrX:32486471-32486744、chrX:32486684-32486958、chrX:32490217-32490489、chrX:32502870-32503090、chrX:32503022-32503275、chrX:32509245-32509496、chrX:32509438-32509693、chrX:32519777-32520051、chrX:32535919-32536193、chrX:32536129-32536357、chrX:32563213-32563487、chrX:32583773-32584038、chrX:32591600-32591851、chrX:32591744-32592018、chrX:32613661-32613933、chrX:32613869-32614060、chrX:32632355-32632627、chrX:32662039-32662313、chrX:32662248-32662484、chrX:32662880-32663141、chrX:32663073-32663314、chrX:32715912-32716186、chrX:32717187-32717446、chrX:32827566-32827808、chrX:32834428-32834685、chrX:32834626-32834803、chrX:32841293-32841563、chrX:32862767-32863041、chrX:32867764-32868006、chrX:33038179-33038407、chrX:33146124-33146397、chrX:33229277-33229547、chrX:33357241-33357514、chrX:33358758、chrX:33743414、chrX:33808136、chrX:33906415、chrX:33961764、chrX:33963972、chrX:33964605、chrX:33964917、chrX:33965241、chrX:34143170、chrX:34149267、chrX:34151292、chrX:34152218、chrX:34162244、chrX:34165401、chrX:34220751、chrX:34222722、chrX:34224894、chrX:34230300、chrX:34319940、chrX:34337889、chrX:34356192、chrY:2655296、chrY:2656696、chrY:6635045、chrY:7072005、chrY:8212252、chrY:14829486、chrY:15995909、chrY:17920298、chrY:19418832、chrY:23282953。采用上述274个SNP位点作为目标SNP位点，SNP分布较均匀适用于大部分的病例，经检测99位女性DMD携带者，通过标准设定为突变位点两侧各有3个以上有效SNP，99名受检者通过率为98％，说明通用性和准确性较高。任选其中28名受检者同时进行了STR筛选，67.9％(19/28)受检者突变位点两侧分别有≥2个有效STR，所以相比之下，本发明的方案适用范围明显更广。

在一个具体示例中，杜氏肌营养不良基因编码区位点和目标SNP位点还包括chrX:31154097、chrX:31158639、chrX:31184563、chrX:31195121、chrX:31209414、chrX:31210629、chrX:31213849、chrX:31224012、chrX:31241026、chrX:31260937、chrX:31263106、chrX:31270444、chrX:31279271、chrX:31292610、chrX:31294957、chrX:31312882、chrX:31332896、chrX:31344293、chrX:31350022、chrX:31353420、chrX:31358952、chrX:31361821、chrX:31364476、chrX:31368181、chrX:31373423、chrX:31379559、chrX:31384174、chrX:31394699、chrX:31415613、chrX:31421666、chrX:31426397、chrX:31430450、chrX:31433585、chrX:31443829、chrX:31447503、chrX:31452809、chrX:31456029、chrX:31460986、chrX:31466913、chrX:31476120、chrX:31479084、chrX:31494514、chrX:31502157、chrX:31517385、chrX:31526200、chrX:31536430、chrX:31540864、chrX:31547777、chrX:31555165、chrX:31556970、chrX:31568255、chrX:31575870、chrX:31596491、chrX:31604807、chrX:31611414、chrX:31634092、chrX:31647841、chrX:31656291、chrX:31678047、chrX:31690979、chrX:31696604、chrX:31702122、chrX:31714994、chrX:31722800、chrX:31733995、chrX:31741909、chrX:31749463、chrX:31758332、chrX:31764406、chrX:31778941、chrX:31788872、chrX:31797950、chrX:31806027、chrX:31815712、chrX:31821551、chrX:31836963、chrX:31848232、chrX:31850266、chrX:31853296、chrX:31868578、chrX:31895821、chrX:31907035、chrX:31951452、chrX:31955085、chrX:31971388、chrX:31978675、chrX:31985835、chrX:31996830、chrX:32009476、chrX:32023800、chrX:32037457、chrX:32050015、chrX:32055629、chrX:32062290、chrX:32071675、chrX:32074173、chrX:32079348、chrX:32092366、chrX:32097831、chrX:32102726、chrX:32106560、chrX:32113786、chrX:32116068、chrX:32118084、chrX:32128015、chrX:32130960、chrX:32143952、chrX:32149400、chrX:32154363、chrX:32159164、chrX:32161599、chrX:32162695、chrX:32165563、chrX:32194441、chrX:32198542、chrX:32205498、chrX:32210177、chrX:32214301、chrX:32217186、chrX:32220853、chrX:32227300、chrX:32229853、chrX:32234233、chrX:32237961、chrX:32243212、chrX:32247578、chrX:32255944、chrX:32257436、chrX:32266542、chrX:32277887、chrX:32286169、chrX:32293091、chrX:32298686、chrX:32305961、chrX:32312128、chrX:32315137、chrX:32318547、chrX:32325854、chrX:32334650、chrX:32344550、chrX:32351661、chrX:32358308、chrX:32368548、chrX:32374317、chrX:32379500、chrX:32394913、chrX:32413107、chrX:32459169、chrX:32464478、chrX:32474756、chrX:32482167、chrX:32497533、chrX:32506001、chrX:32513552、chrX:32526623、chrX:32545066、chrX:32553791、chrX:32587530、chrX:32597275、chrX:32606647、chrX:32626317、chrX:32632856、chrX:32696969、chrX:32707580、chrX:32772054、chrX:32782401、chrX:32788567、chrX:32803196、chrX:32836961、chrX:32844073、chrX:32845169、chrX:32848934、chrX:32849544、chrX:32850514、chrX:32851060、chrX:32852605、chrX:32855686、chrX:32858585、chrX:32859026、chrX:32874098、chrX:32883367、chrX:32889854、chrX:32892236、chrX:32894350、chrX:32902312、chrX:32908878、chrX:32912888、chrX:32920081、chrX:32924871、chrX:32931825、chrX:32934766、chrX:32943912、chrX:32946649、chrX:32949724、chrX:32956232、chrX:32961785、chrX:32966821、chrX:32969683、chrX:32976961、chrX:32990743、chrX:32997855、chrX:33004667、chrX:33016109、chrX:33021600、chrX:33028940、chrX:33036335、chrX:33041735、chrX:33043260、chrX:33044852、chrX:33046344、chrX:33049029、chrX:33053550、chrX:33063642、chrX:33067502、chrX:33071898、chrX:33078480、chrX:33081262、chrX:33087356、chrX:33114553、chrX:33183495、chrX:33186217、chrX:33191555、chrX:33193810、chrX:33199451、chrX:33205918、chrX:33225493、chrX:33240020、chrX:33243198、chrX:33245234、chrX:33253361、chrX:33263128、chrX:33270451、chrX:33276434、chrX:33281297、chrX:33284194、chrX:33289027、chrX:33290894、chrX:33293703、chrX:33300363、chrX:33305643、chrX:33309838、chrX:33314096、chrX:33320612、chrX:33325953、chrX:33330066、chrX:33332108、chrX:33333885、chrX:33347806、chrX:33350105、chrX:33352563。采用上述250个SNP位点与前述274个SNP位点结合作为目标SNP位点，能够更好地适用于基因内发生染色体重组的情况，经检测99位女性DMD携带者，通过标准设定为突变位点两侧各有3个以上有效SNP，99名受检者通过率为99％，说明通用性和准确性得到进一步提高。

在一个具体示例中，杜氏肌营养不良基因编码区位点和目标SNP位点还包括chrX:27137952、chrX:27200005、chrX:27313350、chrX:27340544、chrX:27400507、chrX:27507865、chrX:27600120、chrX:27709974、chrX:27794458、chrX:27937320、chrX:28178679、chrX:28276321、chrX:28323063、chrX:28427123、chrX:28501174、chrX:28602208、chrX:28701597、chrX:28803985、chrX:28900028、chrX:29100743、chrX:29200099、chrX:29310488、chrX:29419670、chrX:29500219、chrX:29599164、chrX:29700378、chrX:29800066、chrX:29902085、chrX:30095290、chrX:30137116、chrX:34237275、chrX:34274276、chrX:34300252、chrX:34333522、chrX:34367301、chrX:34410301、chrX:34444321、chrX:34478466、chrX:34511227、chrX:34544524、chrX:34576912、chrX:34611562、chrX:34645144、chrX:34671988、chrX:34715516、chrX:34745702、chrX:34771605、chrX:34800447、chrX:34832383、chrX:34862926、chrX:34895161、chrX:34927942、chrX:34955744、chrX:34987610、chrX:35068338、chrX:35120804、chrX:35181444、chrX:35193194、chrX:35217757、chrX:35225955、chrX:35234594、chrX:35253818、chrX:35269636、chrX:35278547、chrX:35288047、chrX:35300299、chrX:35312188、chrX:35315359、chrX:35315956、chrX:35316635、chrX:36233578、chrX:36273186、chrX:36300632、chrX:36341870、chrX:36387037、chrX:36401894、chrX:36409899、chrX:36481149、chrX:36601177、chrX:37675062、chrX:37805367、chrX:37924639、chrX:38095027、chrX:38151200、chrX:38288022、chrX:38397382、chrX:38434120、chrX:38490102、chrX:38645667、chrX:38693264、chrX:38723631、chrX:38778703、chrX:38804143、chrX:38852423、chrX:38921384、chrX:39001550、chrX:39173040、chrX:39221335、chrX:39228662。采用上述99个SNP位点与前述274个SNP位点和250个SNP位点结合作为目标SNP位点，能够更好地适用于突变位点在基因两端的情况，经检测99位女性DMD携带者，通过标准设定为突变位点两侧各有3个以上有效SNP，99名受检者通过率为100％，说明通用性和准确性得到进一步提高。

在一个具体示例中，上述多个引物对具有相近的退火温度，且PCR产物的片段大小均为125bp～275bp，扩增效果较好。

在一个具体示例中，上述多个引物对的核苷酸序列分别如SEQ ID NO.1～SEQ IDNO.1246所示，具体如下表1所示。

表1

在一个具体示例中，亲代双方的DNA样本为外周血基因组DNA、精液DNA、口腔粘膜细胞DNA和细胞全基因组扩增产物中的一种或多种。可以理解，子代的DNA样本可来自受精卵在体外发育至卵裂期或囊胚期时的若干细胞，例如囊胚期的滋养层细胞等。优选地，每份DNA样本中的DNA含量大于500ng。

在一个具体示例中，测序分析的方法为Ion Torrent PGM或Illumina Miseq，可以理解，不限于此。可以理解，本发明中当待测的DNA样本来自多个受试样品时，每个样品可以被加上不同的标签序列(barcode)，以用于在测序过程中进行样品的区分，从而实现同时对多个样品进行测序。可选地，基因组参考序列可来自公共数据库，例如人类基因组序列可以是NCBI或者UCSC数据库中的人类基因组参考序列hg19、hg38等。测序分析时序列比对可以通过任何一种序列比对程序，例如本领域技术人员可获得的BWA(Burrow-Wheeler-Aligner)进行比对，将读段与参考基因组序列比对，得到读段在参考基因组上的位置。数据处理时可利用trimmomatic软件对Illumina等测序仪产生的原始数据去除接头序列，BWA软件比对到人类参考基因组，最后分析单体型SNP覆盖倍数和基因型。

本发明一实施例的引物组合物，包括能够特异性扩增上述杜氏肌营养不良基因编码区位点和目标SNP位点的多个引物对。

在一个具体示例中，上述多个引物对的核苷酸序列分别如SEQ ID NO.1～SEQ IDNO.1246所示。

本发明一实施例的用于检测杜氏肌营养不良基因缺陷的试剂盒，包括上述引物组合物。可选地，还可以包括通用引物、多重PCR聚合酶、DNA连接酶、末端修复酶、dNTPs、反应缓冲液等。

以下为具体实施例。

实施例1

一女性DMD携带者已生育一个DMD患儿，患儿dystrophin基因(NM_004006.2)存在c.3397G>T(p.Glu1133Term)半合子突变，遗传自母亲。该女性经PGD助孕，获得4枚胚胎(WXY-1、WXY-2、WXY-3、WXY-4)，胚胎发育至囊胚期进行滋养层细胞活检，活检细胞进行全基因组扩增。

建库和测序

按照Illumina标准建库流程对活检细胞全基因组扩增产物进行建库，用Miseq进行测序，杜氏肌营养不良基因编码区位点和目标SNP位点及相应的引物对如表1所示。

比对与统计

利用trimmomatic软件对Illumina测序仪产生的原始数据去除接头序列，用BWA软件比对到人类hg19参考基因组，最后分析单体型SNP覆盖倍数和基因型。

结果分析

其中，F1表示男方正常染色体，M0表示女方风险染色体，M1表示女方正常染色体。

注：Hom表示此突变位点为纯合突变，Het表示此突变位点为杂合突变，Hemi表示此突变位点为半合子突变，Normal表示此位点未检出突变。

附表4 SNP单体型

以上所述实施例的各技术特征可以进行任意的组合，为使描述简洁，未对上述实施例中的各个技术特征所有可能的组合都进行描述，然而，只要这些技术特征的组合不存在矛盾，都应当认为是本说明书记载的范围。

以上所述实施例仅表达了本发明的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对发明专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进，这些都属于本发明的保护范围。因此，本发明专利的保护范围应以所附权利要求为准。

序列表

<110> 中信湘雅生殖与遗传专科医院有限公司

<120> 杜氏肌营养不良基因缺陷的检测方法、引物组合物及试剂盒

<160> 1246

<170> SIPOSequenceListing 1.0

<210> 1

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1

gtctcaatgt tcctcaggtc act 23

<210> 2

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 2

gggaggaact tgcatttttc tcac 24

<210> 3

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 3

gcctgccaca ggtttcattc tt 22

<210> 4

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 4

agccctgctc tgagtcttct aa 22

<210> 5

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 5

cctcatctgg gtcccagaaa ac 22

<210> 6

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 6

gctgaggaag ttacaatgtg ccttag 26

<210> 7

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 7

ctactgagtg tgatcttctt aaatggca 28

<210> 8

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 8

gtgtcattca ccttggccaa aa 22

<210> 9

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 9

ccttagccca caggttcctc ta 22

<210> 10

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 10

aaccctctgt gtcagggaaa ac 22

<210> 11

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 11

gcaacttatt gactgattgt gacaatgc 28

<210> 12

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 12

gcgcaggtac tgccaatact ac 22

<210> 13

<211> 20

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 13

cctgcgcttg atttgtgctc 20

<210> 14

<211> 21

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 14

gcatcctcta cagcttgctc a 21

<210> 15

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 15

gtttagcaag aactcatttt ccagtttgt 29

<210> 16

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 16

tatgcatcat atgcatgtat cgaaacatct 30

<210> 17

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 17

cccagacctg aaaactcata agca 24

<210> 18

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 18

cacctctgct ctagtaaaca atcgg 25

<210> 19

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 19

tgcatagggt ttctatgcaa attgaatt 28

<210> 20

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 20

gatgcagacc aagtactctg ttct 24

<210> 21

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 21

gggagaaata ggtagtaaag acatgtttgt 30

<210> 22

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 22

gctacctatc tttaccaggt cattttaact 30

<210> 23

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 23

gcttgatagg gcacactctg aa 22

<210> 24

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 24

atcattacag aagcagagat gcattactt 29

<210> 25

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 25

gattgccctt caaaactaag gaaagaaa 28

<210> 26

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 26

ttcagcatct ctccacatta aaatcact 28

<210> 27

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 27

cccaaatatt ctaggtgctt tttcctg 27

<210> 28

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 28

gggaaaggat tcactctgtg aaaga 25

<210> 29

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 29

ccttgctctt aagtgcacaa agga 24

<210> 30

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 30

gctcatactg ttgtatttaa agctttcaca 30

<210> 31

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 31

gaacgtttgt cctttttgcc ctt 23

<210> 32

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 32

tataaatcac aggatcacaa ggagacaca 29

<210> 33

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 33

ggaaacaacc taaatgcaca tggataag 28

<210> 34

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 34

ttttaaagta tcagtttggt gtgtaccctt 30

<210> 35

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 35

atggtttgat tagaactgga aactgga 27

<210> 36

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 36

tccctgtttc ttcatttgtt tgtcct 26

<210> 37

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 37

agctggattg gaacttggaa cag 23

<210> 38

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 38

ccacagacgt gttttacttg gact 24

<210> 39

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 39

caatgtaaat aaaaggactt ggacttgca 29

<210> 40

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 40

gcaaggatta aaataaacaa agggtcttca 30

<210> 41

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 41

tcagaatttc taagcatctc aggtgatg 28

<210> 42

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 42

catccaggaa attgtttttg ctgattttaa 30

<210> 43

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 43

ctttactcct ctgctgcact caa 23

<210> 44

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 44

tgtttagcac ttaatccatc cacagatg 28

<210> 45

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 45

gcacttgacc agtgtcgaag aa 22

<210> 46

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 46

cagactcagt aggtagaaag ggaga 25

<210> 47

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 47

taaaaaccat gcgggaatca gga 23

<210> 48

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 48

caccttttgt aaagtctgtc tttctttctc 30

<210> 49

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 49

tgcacattgg taaatccttc cact 24

<210> 50

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 50

cccgtgaatc cgcttcattt tg 22

<210> 51

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 51

catgatgaca caatgtgtaa tacacacatg 30

<210> 52

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 52

aaatggtcat ctcaacattt gtttggt 27

<210> 53

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 53

ccatcaaaac agagtcagaa atcactg 27

<210> 54

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 54

aaaaagtggt ttttggatgc tttctttc 28

<210> 55

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 55

gtgttggctt ccatatggaa aatacac 27

<210> 56

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 56

ggtgttaaaa atgtaatcat ggcccttt 28

<210> 57

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 57

cgactctacc tttcttcaga caaca 25

<210> 58

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 58

gtcaaaattt atgagtcctg agtgtgtatc 30

<210> 59

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 59

ttgatactat cactttgcag gcacat 26

<210> 60

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 60

gcaaatcctg gaagaccaca ataaac 26

<210> 61

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 61

gctgccttag cctgtgtaac tg 22

<210> 62

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 62

gccaatagga atctgcaagc agaat 25

<210> 63

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 63

ctttatgttc gtgctgctgc tt 22

<210> 64

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 64

agtcagatgc atctatctac catggtatat 30

<210> 65

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 65

ggtcctgagt aggtattaag aatctacaga 30

<210> 66

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 66

acagagatgt atttgtctct gcact 25

<210> 67

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 67

agcattaact ccttcacacc taaaatatga 30

<210> 68

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 68

gctagagaga atcctagcag atcttgag 28

<210> 69

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 69

ccaatgacaa agctagaaag aaaactcac 29

<210> 70

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 70

attttcaaat acactcctga gtccctaac 29

<210> 71

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 71

cctagagttg taactacact tttcttcctg 30

<210> 72

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 72

cccaaccata actgtgatgc ctt 23

<210> 73

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 73

tgctaattcc tatatcctgt gctatcct 28

<210> 74

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 74

tgccatgatt tatcctttag ttttcaggaa 30

<210> 75

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 75

gcaagttgat tagtaggaat cagacaagt 29

<210> 76

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 76

tgtataacat aactgtgtgg tgggttt 27

<210> 77

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 77

tacgacacac tatagcaaat aactaggga 29

<210> 78

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 78

ccagggttct agaagattaa cagattaact 30

<210> 79

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 79

attctcctga tctgtgtaga tggtact 27

<210> 80

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 80

gtgtctcctt caccacctca ttt 23

<210> 81

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 81

ggtgttcagc tgagaggagt tc 22

<210> 82

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 82

agactggagt ggtcattagt tttgaaatc 29

<210> 83

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 83

taggtgagca acattgatta aaagtggt 28

<210> 84

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 84

ctgtaggctt caggatttac cctatc 26

<210> 85

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 85

agtgacaaat cacagcatta tacatgatct 30

<210> 86

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 86

agagtgttct ttgggaattt gattcga 27

<210> 87

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 87

agagtgaagg tggtaaggga ca 22

<210> 88

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 88

caggccaaat gtaacatctg caaa 24

<210> 89

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 89

tttttggttc ctaatacctg aatccaatg 29

<210> 90

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 90

ctttcatcct tttgccctcc ttct 24

<210> 91

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 91

ggaaggaatc agttgtcatt actattgc 28

<210> 92

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 92

cccttcagtg tccaaggatt tatagaaag 29

<210> 93

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 93

gaaaacgaag ctctgtgggt ttt 23

<210> 94

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 94

gttttgactg ttgcaatttt cttcttcc 28

<210> 95

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 95

tgtttcacca acctaaaaac atgttcc 27

<210> 96

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 96

cgagagaaaa ataactgaac agcttttgtt 30

<210> 97

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 97

gttctacatc ttacatgagg aatctgct 28

<210> 98

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 98

taactagaag tgtttaccct ctaggaaagg 30

<210> 99

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 99

gtctcagggt tttgtttgtt ttctaatctg 30

<210> 100

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 100

tctgcgtgga tatgtgtctg aac 23

<210> 101

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 101

aacatgccat acgtacgtat cataaaca 28

<210> 102

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 102

gaaggtttta ctctttgagt catttgtgat 30

<210> 103

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 103

gggcctactt tcctgagact ga 22

<210> 104

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 104

tacctggcca ggtatctcta gc 22

<210> 105

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 105

gttcacagct tctcctaccc aa 22

<210> 106

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 106

ggagatgatg aagttgggct tatct 25

<210> 107

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 107

ggaggaaagc aattctgtca tttttcc 27

<210> 108

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 108

cattcctcag ctactgatgt tgaattaaga 30

<210> 109

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 109

ccctctctaa gtaaaaatgt acccaaccta 30

<210> 110

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 110

gtggttgtag ttattggcaa atcactg 27

<210> 111

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 111

gcatccaatc ccaaattttc tctcatt 27

<210> 112

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 112

agaccatttg ttctaaatgc tgaatagaac 30

<210> 113

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 113

aaaagctctt ctacctaagc actttct 27

<210> 114

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 114

ctgaaggttc tgagccaaag ga 22

<210> 115

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 115

agcacataca tttatatgag ccaccaa 27

<210> 116

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 116

gtgactccag aggtttttct taacaag 27

<210> 117

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 117

gtacattttc ctgctccctt acca 24

<210> 118

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 118

tgacaagagg tggtatcacc aga 23

<210> 119

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 119

gctgcaattc attttgtgag aatctatga 29

<210> 120

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 120

gcccaggctg tgtttttatg tattttt 27

<210> 121

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 121

agcaagtaac tttcacactg caaac 25

<210> 122

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 122

aagcaaaaat catgttgttg ttattgttgt 30

<210> 123

<211> 20

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 123

cgcctgtcca agttttgacc 20

<210> 124

<211> 21

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 124

aagctcactc ctccactcgt a 21

<210> 125

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 125

gaagggtctt gggttcgaat ga 22

<210> 126

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 126

ggcgcactat agatagagag aagct 25

<210> 127

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 127

ccactaggaa aattccaagg ccaaa 25

<210> 128

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 128

tggcccagta cttgatgcta aac 23

<210> 129

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 129

cagcccaatt cccagtcaga ttaa 24

<210> 130

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 130

tcttgcatat tgtctttctt ttccctca 28

<210> 131

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 131

tggcaatttt gttgatgatg aatgagg 27

<210> 132

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 132

ttttaattgc atgaatgccc gatgtt 26

<210> 133

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 133

gcaactgtta acttcatcca ataaggttt 29

<210> 134

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 134

gaacttgcta actgctcagc ttttt 25

<210> 135

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 135

ggtaatatac agtgcatgtc tacagca 27

<210> 136

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 136

ggtagtcttt tgactctttc aggagaaata 30

<210> 137

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 137

gccaggctaa tgtcgcattt ta 22

<210> 138

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 138

gcaaacagac caatatcagt gtaaaattgt 30

<210> 139

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 139

gctttcggcc tgaaagaaac aa 22

<210> 140

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 140

acttaagaac tgcaattaca aaagcttgaa 30

<210> 141

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 141

ttgttggtca tccctctctt gtg 23

<210> 142

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 142

ggaactatat gcgtcacatc aaaagaaaat 30

<210> 143

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 143

agagtgaatt tacctcttct tcttttggg 29

<210> 144

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 144

ttcctctcct ttctgaagat gatttcc 27

<210> 145

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 145

tttcaggatg atttatgctt ctactgca 28

<210> 146

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 146

tgagagaaca tcatttctct ccttttcc 28

<210> 147

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 147

ccatgtttgt ggtgtgacaa gaataaa 27

<210> 148

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 148

tgactatttg ggaacagaac tgagaag 27

<210> 149

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 149

aaaggagatt ctgtgttaca aaaaggtact 30

<210> 150

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 150

tggtgtcata gcagacaatt attgatgg 28

<210> 151

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 151

tcctctttag ggcctttgca tg 22

<210> 152

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 152

ggcataaatg agttaatccc tgcatca 27

<210> 153

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 153

catttctaca cttctgtgcc ctactt 26

<210> 154

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 154

tgttggacat gggaataaaa gttagatgt 29

<210> 155

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 155

acctccaggg tgctgaaaat tt 22

<210> 156

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 156

attatacaaa tgagttaact gggaaggcat 30

<210> 157

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 157

ccaaagaata aggaaacatg ggaaatgaag 30

<210> 158

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 158

gcttcaaaac attcacttct gtgct 25

<210> 159

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 159

caatgattgt tcacatccct gtagtaaaga 30

<210> 160

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 160

cgcatgtctt ttcttaaagg aacacttttt 30

<210> 161

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 161

ccttgctctg tgaacaaagt agaaaa 26

<210> 162

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 162

gtcatccagc tgagggtgtt ta 22

<210> 163

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 163

ctgtaacaaa ggacaacaat gtttacaatg 30

<210> 164

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 164

gcccaggtat cagttcttct tgttttaaat 30

<210> 165

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 165

caccagtata tgccaagcca tagt 24

<210> 166

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 166

agagatgctt cagaaattag caaatgc 27

<210> 167

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 167

cacttcttga gtagaatcag atgaactca 29

<210> 168

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 168

gactgaatga actttgctta aagacagg 28

<210> 169

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 169

aaaaagaaaa caacaatcca caaaccttt 29

<210> 170

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 170

ttaagggcaa gatcatgact tttcact 27

<210> 171

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 171

aacactgcac tcaagttcag attagaa 27

<210> 172

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 172

gagtgggaaa aattgaacct gca 23

<210> 173

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 173

tcaagggtct catctatttt tctctgc 27

<210> 174

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 174

gcctaaaacc ttgtcatatt gccaa 25

<210> 175

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 175

tatccagacc ctggcagcaa ga 22

<210> 176

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 176

caggattaat tttgagaaga atgccaca 28

<210> 177

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 177

acagagagga taacaagaca aagggata 28

<210> 178

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 178

accttctgtc aaggtcgtaa catatatct 29

<210> 179

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 179

tccattcatt gtttcagata gtcaacaaca 30

<210> 180

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 180

cccttgatct cagagaatac aatctttgtt 30

<210> 181

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 181

gcccataggc ttcaagagca tc 22

<210> 182

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 182

gaatggtgct tcaataaaca tacgaca 27

<210> 183

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 183

agtcactgga ttactatgtg cttaacatg 29

<210> 184

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 184

acatggtacg ctgctgttct tt 22

<210> 185

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 185

gacatgctat ttgccacttt caattct 27

<210> 186

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 186

aaagataggg aggctctatt agctttcta 29

<210> 187

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 187

ggccatttta attcatttgt ggcctt 26

<210> 188

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 188

tgtttctttt gtttggtaat tctgcacat 29

<210> 189

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 189

ttttaggctt gacaggtgga aagt 24

<210> 190

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 190

tcattctgtc aacagaatgt aagcaaag 28

<210> 191

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 191

aaaaagcatc ggcttaaatt ttgtatgtct 30

<210> 192

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 192

aactgcattt tctgatctgg ttagga 26

<210> 193

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 193

gagatggtag tttcttattg gatcagttct 30

<210> 194

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 194

tgtgacaaag aaccatgtta agaaaacc 28

<210> 195

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 195

cccaaactga ttttgcagta tcttttatca 30

<210> 196

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 196

agctgctcta ggatcctatt taacaga 27

<210> 197

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 197

gtttaacata acctaaccct gggaagat 28

<210> 198

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 198

tgtgatttga ccattttgga actaaaaagt 30

<210> 199

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 199

aaaaacccaa gcatcatatt ctgatgac 28

<210> 200

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 200

taacatacat tgaaataaag gccagagcat 30

<210> 201

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 201

tgaacagatc ttcttttgta ctttctgtgt 30

<210> 202

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 202

agagcttctg taatacagaa agtatgtctt 30

<210> 203

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 203

tctcaacaat ttctctgggc aagtt 25

<210> 204

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 204

tgcttcttat tttacccttt tgtcagagaa 30

<210> 205

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 205

gattccctgt cctaaatgac agattact 28

<210> 206

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 206

tattgtgtca atatgtgtgg gagtctct 28

<210> 207

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 207

gtaaaactct aggcatggga ccat 24

<210> 208

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 208

tcagccattt tcagttgcat ttttacttta 30

<210> 209

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 209

tgaacaatcg agtttatttt gctgattcac 30

<210> 210

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 210

cactagtcta ggaaaacact agtctgaaaa 30

<210> 211

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 211

ttgcctggct gtgtgtatat cttt 24

<210> 212

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 212

ctcctgagaa caatttgttt agtaccga 28

<210> 213

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 213

taacacacct acattgtatt cccaaattga 30

<210> 214

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 214

tcaacgagtt agttaaaatg gaaggctaat 30

<210> 215

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 215

aatagctctt cttaaaagtc acattcagga 30

<210> 216

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 216

tacaaatatt tggaaaatgg ctgtgcaa 28

<210> 217

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 217

ggaataaaac acaatggtta ttggaggcta 30

<210> 218

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 218

accatgtctc attcatacgt aaacatgtta 30

<210> 219

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 219

caaatgtttt cctggtcaga gcat 24

<210> 220

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 220

caagggagta aaagagctga tgaaac 26

<210> 221

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 221

gctaaagcgg aaatgcctga ct 22

<210> 222

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 222

ggtgcaccat tctgatattt aataattgca 30

<210> 223

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 223

acctagaatc aaaaggaaaa gactagagga 30

<210> 224

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 224

aactcagatc tgcctagttt ttaaaactct 30

<210> 225

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 225

ctcctttgac aggatccaca ga 22

<210> 226

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 226

acaaagggct tttgagaata gtgacatatt 30

<210> 227

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 227

cccaagtact ttcgagagtt gact 24

<210> 228

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 228

gttaaactta gtcctggaac ctgcta 26

<210> 229

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 229

tgaaaagata aacacctgtc catttcct 28

<210> 230

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 230

aacttctttc ctttagtaca agtgattctt 30

<210> 231

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 231

gggcaagata atgcattcta cttgac 26

<210> 232

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 232

gcaagttctc tgtcttcaat gcaattttaa 30

<210> 233

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 233

cgggcaaatg agatcttatg ttcc 24

<210> 234

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 234

accagaaaag tccacatgat aacagag 27

<210> 235

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 235

tcataccttt tatgaatgct tctccaagag 30

<210> 236

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 236

gctgtttacg aagtatttta agacactcca 30

<210> 237

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 237

gcttagccta tgctgactga ca 22

<210> 238

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 238

tatggtcaag ataggcaggt tctgt 25

<210> 239

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 239

ctaaaacctt gtcctcatag cagct 25

<210> 240

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 240

ttccaatttt gtgacacttg tcattttca 29

<210> 241

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 241

caaagtctac tgttcatttc agctttaacg 30

<210> 242

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 242

atggaaggag ggtccctata cag 23

<210> 243

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 243

actaaccttg gtttctgtga ttttcttttg 30

<210> 244

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 244

gtctcctcca gactagcatt tactactata 30

<210> 245

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 245

gatatcacct catatctttt ggattggct 29

<210> 246

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 246

ctcttcaata cactccctcc ttttct 26

<210> 247

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 247

agtatgtatt gcttcccata cggaaag 27

<210> 248

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 248

aagcttttct cctaacttca ggctt 25

<210> 249

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 249

gcacatgcac cttctgtatc taaaagt 27

<210> 250

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 250

catagaatta aagggagatt cgggctta 28

<210> 251

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 251

ccaggttacc acacactttg aaaag 25

<210> 252

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 252

gtctttattc ttacacagca taagtcctga 30

<210> 253

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 253

ttttattgaa acttgtcatg catcttgct 29

<210> 254

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 254

tatgtaaaag gaatacacaa cgctgaagaa 30

<210> 255

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 255

tttttaagtg cttctgttgt gtcact 26

<210> 256

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 256

agaaaagctg aggactactt aagcttg 27

<210> 257

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 257

gagatctctc tggaaataca acttcaagg 29

<210> 258

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 258

cgtgaaaagg ttgaatgcat ttttaacaaa 30

<210> 259

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 259

cagcacaatg atgaacattt caggt 25

<210> 260

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 260

taccctccta atttcatggt aactcca 27

<210> 261

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 261

gcatatgtag agaaatcaag tgactcaga 29

<210> 262

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 262

catgcataga cgttgcctat cctag 25

<210> 263

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 263

gtctaaggtt tggtctgaat ctcataact 29

<210> 264

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 264

tcacaacgtg tagacctgaa ataaagac 28

<210> 265

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 265

cttaggctga atagtgagag taatgtgttt 30

<210> 266

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 266

aacttaccga ctggctttct ctg 23

<210> 267

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 267

ccaccatcac cctctgtgat tttata 26

<210> 268

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 268

ggctctttag cttgtgtttc taatttttct 30

<210> 269

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 269

aacaatttga gccaaactct tattcatgac 30

<210> 270

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 270

taaaatttat ttccctggca aggtctga 28

<210> 271

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 271

cagtagcaac tgcagtgtgg at 22

<210> 272

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 272

cagcgcctgg cttataatta actttaaaaa 30

<210> 273

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 273

aagatgcata ggagagaaga aatgaatgag 30

<210> 274

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 274

ctcctaaata tttcttggtt cagctttcat 30

<210> 275

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 275

tgcatttatt tctgattaac ttcctgcaa 29

<210> 276

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 276

aacagatctg agcattaagt caagatgtt 29

<210> 277

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 277

ttctctctca cccagtcatc act 23

<210> 278

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 278

attcaccaaa tggattaaga tgttcatgaa 30

<210> 279

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 279

tcttctcatg gtgtaagtat ctcagtga 28

<210> 280

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 280

gtagtgaatt aggaacgaaa cctaagactt 30

<210> 281

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 281

tcggaaccac aatgttgtat attagcc 27

<210> 282

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 282

acccagccag atgtgatctt ttaaaaa 27

<210> 283

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 283

gtccacgtca atggcaaatg tac 23

<210> 284

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 284

tacatgtgga gtctccaagg gtatattt 28

<210> 285

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 285

tcagaggtat gcctgtatat gtgtataca 29

<210> 286

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 286

cgccttttgg caatggaact tg 22

<210> 287

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 287

tgatagacaa gaaggtcagg acaga 25

<210> 288

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 288

agtgccattc ccttatttaa aactcttgta 30

<210> 289

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 289

tgtcatctgt tcatatactc tttcagatgc 30

<210> 290

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 290

tgcaaatatc actggccact attca 25

<210> 291

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 291

ctaaaggctc actaattgga gtgct 25

<210> 292

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 292

agactaaaat gcctgtgtgt gtgt 24

<210> 293

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 293

ttcaaatttt tctcccacca tcctga 26

<210> 294

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 294

tgaacttttt gcatggccta caaag 25

<210> 295

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 295

caatctctct ctatccaacc tcctgta 27

<210> 296

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 296

tggttatctc ctattaggaa tcagttggaa 30

<210> 297

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 297

tcaaatggtc cttcttggtt tggt 24

<210> 298

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 298

ggcttatgcc ttgagaatta tttacctttt 30

<210> 299

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 299

gatggacctt caccaaaagt ttgttaaa 28

<210> 300

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 300

cccttagaca tgagtcacaa gca 23

<210> 301

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 301

actaactatg gcacaggcaa tagc 24

<210> 302

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 302

aaaaacttag tgtatccatg ctactacca 29

<210> 303

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 303

aaagtaagtg ggatgataga gcccta 26

<210> 304

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 304

ggaaaagaag acattttaat ggcagagt 28

<210> 305

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 305

ttgatactgg gtgtgtaaaa tagactcaaa 30

<210> 306

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 306

tcattgttct agataaacaa tggcaatgtt 30

<210> 307

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 307

agaagcacgt ataaaaatgg taagtgcta 29

<210> 308

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 308

gggtgaaaga tgattgactg ataaacaga 29

<210> 309

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 309

acaaatactt gcaacattta acacatgtga 30

<210> 310

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 310

ggtagttgga attgtgctgt aattcatttt 30

<210> 311

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 311

tcaaatttgg gtagtttcat tctggagt 28

<210> 312

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 312

ccttttgaat acctggagag ttggt 25

<210> 313

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 313

acatatgttc ttatgtgcac tggttca 27

<210> 314

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 314

ccacttgaac ctggaaaaga gc 22

<210> 315

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 315

taaaattacc ttgacttgct caagcttttc 30

<210> 316

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 316

cccagtttgc attaacaaat agtttgagaa 30

<210> 317

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 317

ctgggagcag ggtctgtttt taa 23

<210> 318

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 318

ttcaaaagtt agtttaccgt gttttctctg 30

<210> 319

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 319

attcatgaac atggaatatg gtcccattta 30

<210> 320

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 320

ctgctagatc tgataagttc atgaagct 28

<210> 321

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 321

gtggctgttg atattctttc tctgtca 27

<210> 322

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 322

cagcatatct gcaaaatgag gca 23

<210> 323

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 323

gtgatatggg ataagcaata tcagacttgt 30

<210> 324

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 324

ctggagtatt ctaggagaag acatacca 28

<210> 325

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 325

tttactgctg ttgattaatg gttgataggt 30

<210> 326

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 326

caatcctcaa aaacagatgc cagtat 26

<210> 327

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 327

cgcagattca ggcttcccaa tt 22

<210> 328

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 328

cttcattttt gttttgcctt tttggtatct 30

<210> 329

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 329

tcaggacaaa tagaaaacat ttaagggct 29

<210> 330

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 330

aatatcacgt cctgagacag tgttttt 27

<210> 331

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 331

gctatgataa aagacagtaa ctcaaaggct 30

<210> 332

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 332

cacagttcat cacttattat cctggcttat 30

<210> 333

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 333

aagatttctg taagccgttc ctttct 26

<210> 334

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 334

gcataaattg ttccctttgt gctct 25

<210> 335

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 335

catgcacagg gataaaacag gaag 24

<210> 336

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 336

ggcatcaccc tatggatgga gta 23

<210> 337

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 337

tgccaaagaa ttctttcata gtctgattct 30

<210> 338

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 338

gtaagctgct tccaggaaaa gaaag 25

<210> 339

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 339

gcttaaatat aatctgagat cacccaccat 30

<210> 340

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 340

tccaccacaa accataatta cgtcc 25

<210> 341

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 341

ggtaatgcaa cgtgttagta attgct 26

<210> 342

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 342

tatatcagca cccaaccgct caa 23

<210> 343

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 343

ctttatatga gctggccatt gccta 25

<210> 344

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 344

ggaaggcttc tttttgctta tattgattct 30

<210> 345

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 345

caagagtcct actgattgtt aacaggt 27

<210> 346

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 346

cactggagtt ttaacaatat tgtgtgttca 30

<210> 347

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 347

cgggcagtgt tgacagatca aa 22

<210> 348

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 348

ttcttgtttc cggtcacagt tca 23

<210> 349

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 349

atcctgacac aaaaagtcca tagca 25

<210> 350

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 350

ctaacagaag ctgaacagtt tctcaga 27

<210> 351

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 351

atttagcatg ttcccaattc tcagga 26

<210> 352

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 352

gttacttgaa actaaactct gcaaatgca 29

<210> 353

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 353

tttctacagt tcagctcatt tgtctgaa 28

<210> 354

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 354

cccagcttga tttccaatgg gaa 23

<210> 355

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 355

cccttgtcgg tccttgtaca tt 22

<210> 356

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 356

accatttgct acctttggga tttgtatata 30

<210> 357

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 357

tctttctacc ctagatttct gaagcca 27

<210> 358

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 358

ctaaggactt tgaagatctc tttaagcaag 30

<210> 359

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 359

cgaatgaaag tgctttggtt ttacctt 27

<210> 360

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 360

ggaggtttca ctgttaggaa gctaaaaa 28

<210> 361

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 361

cacatacgtg ggtttgccag ta 22

<210> 362

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 362

tcggttgatg tggttagcta actg 24

<210> 363

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 363

gaaaacaaca cacaatacaa ggaaatgc 28

<210> 364

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 364

tgatgatctc ctgaaatgct tggatg 26

<210> 365

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 365

gctcaggtag gctggctaat tt 22

<210> 366

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 366

gcagccagaa gtgcactata catatatatt 30

<210> 367

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 367

gccataactt ttaagcaaca catcgt 26

<210> 368

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 368

gatgagagaa agcgagagga aataaagata 30

<210> 369

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 369

ccttgagagc attatgtttt gtctgtaac 29

<210> 370

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 370

accaaaatga agactgtact tgttgttttt 30

<210> 371

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 371

tttccactcc tagttcattc acactttt 28

<210> 372

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 372

agtttagcaa caggaggttg aataaatgta 30

<210> 373

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 373

gcaaccttcg caagagacca tt 22

<210> 374

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 374

tactaacttc aagtcctatc tcttgctca 29

<210> 375

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 375

cgtccttagt atcttttagg acaaagatga 30

<210> 376

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 376

agaatattgt ctaaccaata atgccatggt 30

<210> 377

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 377

tctcgtgaca gagaagggtg ta 22

<210> 378

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 378

ctacaagaca ttacttgaag gtcaatgc 28

<210> 379

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 379

tcacgtatgt tcaaaataac cttcagtga 29

<210> 380

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 380

aaatgcttga aattgtcccg taagatg 27

<210> 381

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 381

ccagccattc tgtcaagaca ttc 23

<210> 382

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 382

ctgccctaga cagtttttat tcactcttaa 30

<210> 383

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 383

catagagaga gagaggtgtt tagaattgc 29

<210> 384

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 384

gtgataaaga ctggacgtca gattgt 26

<210> 385

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 385

tcaagttctt tgggattttc cgtct 25

<210> 386

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 386

cagaaaatgc aaaagctaga tattgaccac 30

<210> 387

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 387

ctgcgtattt gccaccagaa at 22

<210> 388

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 388

gcaaaattaa atcagtgcct ttttacactg 30

<210> 389

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 389

caatcttgcc aataatcaag ttgtcca 27

<210> 390

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 390

tctgaatttc gtcttcttgg aaagttagt 29

<210> 391

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 391

acaaaagaat ggaagctgat tccca 25

<210> 392

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 392

gcaggattac agaaaagcta tcaagagtaa 30

<210> 393

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 393

ccttacagcc taaaaagaag gaataagagt 30

<210> 394

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 394

ccagcctatt tccttaatgt acgtgatatt 30

<210> 395

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 395

ggcactcatt cagctctgtt ga 22

<210> 396

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 396

gatgacgatg accctattct aagca 25

<210> 397

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 397

acacatgtaa agaatttacc cagtgtct 28

<210> 398

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 398

gagctaatca atgaggaact tgagaca 27

<210> 399

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 399

atacctcttc atgtagttcc ctccaa 26

<210> 400

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 400

ctgagcattt gctgataatc caatgtattt 30

<210> 401

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 401

aaaatcttgt gacctagaag gttattaggg 30

<210> 402

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 402

gcaaacaagt ggctaaatga agtagaa 27

<210> 403

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 403

gccaggaatg ttttcagtgg tt 22

<210> 404

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 404

tgaattacct gctacaaagt aaaggtgaaa 30

<210> 405

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 405

gaaaacaggt ttacacaagt taagcaaatg 30

<210> 406

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 406

cctgtagcac aagaggcctt aaaaa 25

<210> 407

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 407

cactggtagt tggtggttag agt 23

<210> 408

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 408

tctaactggg atgttgtgag aaagaaatt 29

<210> 409

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 409

atcaacctct tctcttagaa ccagga 26

<210> 410

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 410

gctgaagaag agtatcttga gagagatttt 30

<210> 411

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 411

ttcaactgct ttctgtaatt catctgga 28

<210> 412

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 412

tcactgtcaa taatcgtgtt ttgtttgttt 30

<210> 413

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 413

ttgtgcttta aagcaaaaac ataggaaca 29

<210> 414

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 414

agacttgaga cagaactcaa agaacttaac 30

<210> 415

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 415

gtctatacct gttggcacat gtga 24

<210> 416

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 416

ccatcagtcc caattttaca ttttctagct 30

<210> 417

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 417

cccagctgta aaacactgat ctaacc 26

<210> 418

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 418

tgtgttaaaa gtaatcagca caccagta 28

<210> 419

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 419

aaaagactca tgtcttttca tcttctcact 30

<210> 420

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 420

ctctgaaatt agccggaaat atcaatcaga 30

<210> 421

<211> 20

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 421

cttcttccag cgtccctcaa 20

<210> 422

<211> 33

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 422

aactgataga agatcatcta ctttgtttac atg 33

<210> 423

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 423

gttatatcaa tgtgaatgct tgataagcgt 30

<210> 424

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 424

ggaaaacatg gcaaagtgtg aaacaatta 29

<210> 425

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 425

agacagctta tctgttacac cagtatttc 29

<210> 426

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 426

cattttaagc aagtcttttc tgatgtgca 29

<210> 427

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 427

ggctttttac ttacttgtct gtagctctt 29

<210> 428

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 428

gcaaaatgta atgtatgcaa agtaaacgtg 30

<210> 429

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 429

ctgttgctta cattttgaac tttattgcg 29

<210> 430

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 430

acaattggag cagatgacaa ctactg 26

<210> 431

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 431

gggtggtggg ttggattttc aa 22

<210> 432

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 432

atcatttctt tcagtctgtg ggttca 26

<210> 433

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 433

acaagtgctt gtctgatata attcagct 28

<210> 434

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 434

cttaaggctt gaaagggcaa gtaga 25

<210> 435

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 435

ctagtcagtc acagaagaat ccagaaa 27

<210> 436

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 436

ttgcagagtc ctgaatttgc aatc 24

<210> 437

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 437

actttttctt ttaagtctga gaagttgcct 30

<210> 438

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 438

caggagtctc agattgagaa aagaatgata 30

<210> 439

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 439

caaaactgct gtaaatgagt tttctcca 28

<210> 440

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 440

agcaatgtct gacctctgtt tcaa 24

<210> 441

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 441

aaaaatctct gagatagtct gtagcatgat 30

<210> 442

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 442

gcaacccagg cttattctgt gat 23

<210> 443

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 443

acagtactgg gtttttataa gaccattga 29

<210> 444

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 444

tgtgagaaac tagctgtaaa agacacg 27

<210> 445

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 445

ctaaaaaggc actgcaagac attaaaga 28

<210> 446

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 446

taaaacgtag ttaccaattg tttgctgatg 30

<210> 447

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 447

gattttaccc atccgcagtt agttact 27

<210> 448

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 448

gtggtagttg atgaatctag tggagatc 28

<210> 449

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 449

ctgaccttaa gttgttcttc caaagc 26

<210> 450

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 450

agcaaatcat ttcaacacac atgtaagaat 30

<210> 451

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 451

gtgtataggt actatacaca gagtttgct 29

<210> 452

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 452

tgttcagtaa taagttgctt tcaaagaggt 30

<210> 453

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 453

cagtgcatct atctaacatc tgctct 26

<210> 454

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 454

cagatgaatc tcctaaattc aagatgggaa 30

<210> 455

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 455

cttgctttgt ttttccatgc tagct 25

<210> 456

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 456

cacaccgatt tacctagagt tctaattaca 30

<210> 457

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 457

ctgttggccg atcaggtaga aa 22

<210> 458

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 458

agcacaagga gagatttcta atgatgtg 28

<210> 459

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 459

tagtttacct catgagtatg aaactggtct 30

<210> 460

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 460

gcaaagacat taattgtgta acacccaatt 30

<210> 461

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 461

gcaagtaaaa gcagtgttag attatcttgg 30

<210> 462

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 462

agtagtcctt tcgggttact tatggt 26

<210> 463

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 463

cagaaaacat cttgaatagt agctgtcctt 30

<210> 464

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 464

accaaaaatt gatgtgtagt gttaatgtgc 30

<210> 465

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 465

actctaccat actaaaagca gtggtagt 28

<210> 466

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 466

gcatggaagt aaatctcatg gaacattcta 30

<210> 467

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 467

attagttcat tccaatggaa cgttagatca 30

<210> 468

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 468

gtccgacaat caactcgtaa ttatcca 27

<210> 469

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 469

ccagctggtg gtgaagttga tt 22

<210> 470

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 470

tgctcaagga atgcattttc ttatgaaaat 30

<210> 471

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 471

gcactatgga gcagggtttg tt 22

<210> 472

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 472

ttattgcaac taggcatttg gtctct 26

<210> 473

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 473

tgctctcagt aagaactggt ctga 24

<210> 474

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 474

ttcatttcta gtagattgtc ggtctctct 29

<210> 475

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 475

tgtcataaaa ctatgttgtc agtttctggt 30

<210> 476

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 476

gggataatcg tgaaaatgta tcattggaa 29

<210> 477

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 477

aagatacaca ggtacatagt ccattttgaa 30

<210> 478

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 478

cacatcataa tggaaagtta ctttggttgt 30

<210> 479

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 479

ggaacaggag tcatccaaat atatccc 27

<210> 480

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 480

actctacctc caggtttacc atacc 25

<210> 481

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 481

aacatttatg cttctttgca aactactgt 29

<210> 482

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 482

tttttaaagc tgctgaagtt tgttggt 27

<210> 483

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 483

agcaacactg tttgcagagt ttg 23

<210> 484

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 484

ccagcagtcg gcagtaatag aa 22

<210> 485

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 485

tgtgtgcttt gtgtgcaagt ac 22

<210> 486

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 486

ccagtatttt cagagttgtg tggaatgata 30

<210> 487

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 487

ccagttctat taaccttgga gctgaa 26

<210> 488

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 488

aatggtgctt cggacagttt tg 22

<210> 489

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 489

ccctgttgtt gcaaatgaca gtacta 26

<210> 490

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 490

gtacagccag tatgaagaat catttgc 27

<210> 491

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 491

gctataatta ctggacctac aggagatgt 29

<210> 492

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 492

caggctttta cacttctcac ttcaag 26

<210> 493

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 493

gattcaacat ttacatattg tttctctgct 30

<210> 494

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 494

tggtttttcc acataaagag tatcagtcaa 30

<210> 495

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 495

agtggaatag tatagagagg caggaaa 27

<210> 496

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 496

tcacctagaa aacttgtttg ccctt 25

<210> 497

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 497

gtcccttgaa gattcatgta gagca 25

<210> 498

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 498

tctgtcattg cattttggga taaggtaa 28

<210> 499

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 499

ccagagaaaa caaaagagaa aggattcc 28

<210> 500

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 500

tctgggtgtg actcacagaa ct 22

<210> 501

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 501

tgatgtcacc aaaattgcag agtagat 27

<210> 502

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 502

aaaaagaaat tgtgagtcct aaagcaatga 30

<210> 503

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 503

tgtgttgtcc tgaaattttt ctggttg 27

<210> 504

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 504

tctcaaaaag ccagagtgtg tgt 23

<210> 505

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 505

gtatagtagg agattggaca cctgact 27

<210> 506

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 506

cgcctagagc attccaagac tc 22

<210> 507

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 507

ttttgaaggt caatttctgg acactct 27

<210> 508

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 508

aaatcctaat caaattccca acaggact 28

<210> 509

<211> 33

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 509

atctatataa catgactttg tatagtgtct gga 33

<210> 510

<211> 33

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 510

ggtcttacca acatcacatt atgaaatcta cta 33

<210> 511

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 511

attctattgg caaagaaaat gaaccaaaac 30

<210> 512

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 512

ggcatgaaca catctggtat tcaataatt 29

<210> 513

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 513

ctgagccagt cgcaagagaa ga 22

<210> 514

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 514

aggaggctgt agttgacact ct 22

<210> 515

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 515

gccttcctgt gataaatatc agttcatca 29

<210> 516

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 516

ctgcaaatgg tttaagtgca ggat 24

<210> 517

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 517

gccttttagc aatattccct ttgtactc 28

<210> 518

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 518

aaaacaatcc tccattgata ccaaagga 28

<210> 519

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 519

gcttctgtgc atttttggtg ttc 23

<210> 520

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 520

gcaggcacaa actcagaaac tg 22

<210> 521

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 521

tcagacaaac tttgcataga ctttttgc 28

<210> 522

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 522

tttcagtgga atcaagtgga aaaacaatt 29

<210> 523

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 523

tatgacagct gccatgactt agc 23

<210> 524

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 524

gcaatggaat ataaagtcac agtgagaaac 30

<210> 525

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 525

gttatttcca ggtaacgacc taaatctga 29

<210> 526

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 526

gcatagcttg ccttttaact acctg 25

<210> 527

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 527

ctgcttactg ctttattgtc tatccca 27

<210> 528

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 528

agtattgaaa tctttttgtt cccagtccat 30

<210> 529

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 529

cctgtacaac ctgttgtcca gt 22

<210> 530

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 530

gcgcaagatg gctctagaga at 22

<210> 531

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 531

aagagaaatg acaagatttc tttgctgaa 29

<210> 532

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 532

accacatttg gttctatgaa ctcttttcat 30

<210> 533

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 533

gttttactac ttctgggagc agagtt 26

<210> 534

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 534

tggtggtagt aattagaatg aaaaagcga 29

<210> 535

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 535

gttcaaccac atcataacac tacaagc 27

<210> 536

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 536

gaaccggaac atgagctaac ttct 24

<210> 537

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 537

aattatatct gggcctatca cctgagt 27

<210> 538

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 538

ggctgtctta ggacagcaac aa 22

<210> 539

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 539

agatatgtat ggataggctt gactgtga 28

<210> 540

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 540

acagataggg atactttgga aatgacaatg 30

<210> 541

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 541

tggagaataa tgaagcaagg catca 25

<210> 542

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 542

cctacaacct gtgcctcagt tac 23

<210> 543

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 543

gtcagaggac attcaatacc agca 24

<210> 544

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 544

gtggtggtac cttctggtct tta 23

<210> 545

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 545

cagaaagaga gacagaaagg ttatcatctg 30

<210> 546

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 546

acatggcaac agaaatttgc tgatataatt 30

<210> 547

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 547

tctgggttcc tcatctatgg ca 22

<210> 548

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 548

gaaccaatct ctgaggttca tacacatatt 30

<210> 549

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 549

gaacttaatt agggacaacc tcaggtt 27

<210> 550

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 550

gaacttaatt agggacaacc tcaggtt 27

<210> 551

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 551

cgcccaccaa catttttctt tgg 23

<210> 552

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 552

cgcccaccaa catttttctt tgg 23

<210> 553

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 553

ggaggtgtca tgaataagaa caattaatgg 30

<210> 554

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 554

ggaggtgtca tgaataagaa caattaatgg 30

<210> 555

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 555

atgcatttat cacgacaggg tttttaaaaa 30

<210> 556

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 556

atgcatttat cacgacaggg tttttaaaaa 30

<210> 557

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 557

ccaagcaagg taaagcaggt ct 22

<210> 558

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 558

ccaagcaagg taaagcaggt ct 22

<210> 559

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 559

agggatcatt tacatgaggt tgtacac 27

<210> 560

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 560

agggatcatt tacatgaggt tgtacac 27

<210> 561

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 561

agagattggg acttagtgag atgataca 28

<210> 562

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 562

agagattggg acttagtgag atgataca 28

<210> 563

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 563

ggagacagag tggcttttat ctcc 24

<210> 564

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 564

ggagacagag tggcttttat ctcc 24

<210> 565

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 565

caacatcagt agctgaggaa tggt 24

<210> 566

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 566

caacatcagt agctgaggaa tggt 24

<210> 567

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 567

gcaggcaaca ataagatatg tgtgtg 26

<210> 568

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 568

gcaggcaaca ataagatatg tgtgtg 26

<210> 569

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 569

ttctataccc tcagtaaacg tttttctcc 29

<210> 570

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 570

ttctataccc tcagtaaacg tttttctcc 29

<210> 571

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 571

gggaaagtca atggatccta atcaaag 27

<210> 572

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 572

gggaaagtca atggatccta atcaaag 27

<210> 573

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 573

gtctcgtggc taaaacacaa aaca 24

<210> 574

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 574

gtctcgtggc taaaacacaa aaca 24

<210> 575

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 575

gttcatattc tcagacagca gaaacct 27

<210> 576

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 576

gttcatattc tcagacagca gaaacct 27

<210> 577

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 577

tgcaggatat tgacacaatg ggat 24

<210> 578

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 578

tgcaggatat tgacacaatg ggat 24

<210> 579

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 579

tacttctgca gtctaattag ctgactga 28

<210> 580

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 580

tacttctgca gtctaattag ctgactga 28

<210> 581

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 581

gcaacgtgac agcactgatt ta 22

<210> 582

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 582

gcaacgtgac agcactgatt ta 22

<210> 583

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 583

gctttcggcc tgaaagaaac aa 22

<210> 584

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 584

gctttcggcc tgaaagaaac aa 22

<210> 585

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 585

cagtccacac catttgctga ac 22

<210> 586

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 586

cagtccacac catttgctga ac 22

<210> 587

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 587

cccagttcct agatcagagt ttgatt 26

<210> 588

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 588

cccagttcct agatcagagt ttgatt 26

<210> 589

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 589

tattgagtgt ctactatgtg atagccact 29

<210> 590

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 590

tattgagtgt ctactatgtg atagccact 29

<210> 591

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 591

gtaaaaggct gcctctgtct tct 23

<210> 592

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 592

gtaaaaggct gcctctgtct tct 23

<210> 593

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 593

caatcctggc tccctcatct ac 22

<210> 594

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 594

caatcctggc tccctcatct ac 22

<210> 595

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 595

aaaccaactc tggccttcca at 22

<210> 596

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 596

aaaccaactc tggccttcca at 22

<210> 597

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 597

ttttagattc tcaagggctt ccactt 26

<210> 598

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 598

ttttagattc tcaagggctt ccactt 26

<210> 599

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 599

aatgattcca ccagcatttt caagg 25

<210> 600

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 600

aatgattcca ccagcatttt caagg 25

<210> 601

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 601

cagattggga gagtccacga aa 22

<210> 602

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 602

cagattggga gagtccacga aa 22

<210> 603

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 603

tacagagagc agagtcaagg tca 23

<210> 604

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 604

tacagagagc agagtcaagg tca 23

<210> 605

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 605

ggcatatcca ctgacaacac aac 23

<210> 606

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 606

ggcatatcca ctgacaacac aac 23

<210> 607

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 607

acgttaaatc cgctgttctg aaataatatc 30

<210> 608

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 608

acgttaaatc cgctgttctg aaataatatc 30

<210> 609

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 609

ccctgttagc aatgacaggc at 22

<210> 610

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 610

ccctgttagc aatgacaggc at 22

<210> 611

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 611

tctctgtaaa acatctttgt gcaagtact 29

<210> 612

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 612

tctctgtaaa acatctttgt gcaagtact 29

<210> 613

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 613

cacggatagt attcagccat ctaaaatca 29

<210> 614

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 614

cacggatagt attcagccat ctaaaatca 29

<210> 615

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 615

gccagtgatt ggcttaaaga ggt 23

<210> 616

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 616

gccagtgatt ggcttaaaga ggt 23

<210> 617

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 617

aaccatttat cagtattgct catggttct 29

<210> 618

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 618

aaccatttat cagtattgct catggttct 29

<210> 619

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 619

attactgtct gtcatcccaa gaaacc 26

<210> 620

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 620

attactgtct gtcatcccaa gaaacc 26

<210> 621

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 621

tttttggtac taagtctttg catcctga 28

<210> 622

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 622

tttttggtac taagtctttg catcctga 28

<210> 623

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 623

gggactaatg tggccattgg tt 22

<210> 624

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 624

gggactaatg tggccattgg tt 22

<210> 625

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 625

cactacacct aaaatgtcaa aaagcaca 28

<210> 626

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 626

cactacacct aaaatgtcaa aaagcaca 28

<210> 627

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 627

tacacagaag agggctaaaa aggattg 27

<210> 628

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 628

tacacagaag agggctaaaa aggattg 27

<210> 629

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 629

cgttaaaaat taatactggg aaggcaagt 29

<210> 630

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 630

cgttaaaaat taatactggg aaggcaagt 29

<210> 631

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 631

gaacagcatt tgcaaaggca gatat 25

<210> 632

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 632

gaacagcatt tgcaaaggca gatat 25

<210> 633

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 633

caactactat gtatcagaca ctgttctagg 30

<210> 634

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 634

caactactat gtatcagaca ctgttctagg 30

<210> 635

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 635

ggaaaagcag caaatggtag aaaaga 26

<210> 636

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 636

ggaaaagcag caaatggtag aaaaga 26

<210> 637

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 637

cacattcctg ggagtctgtc at 22

<210> 638

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 638

cacattcctg ggagtctgtc at 22

<210> 639

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 639

tggacccagt ggagaactaa tgt 23

<210> 640

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 640

tggacccagt ggagaactaa tgt 23

<210> 641

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 641

tagacacagg atgagaaaaa gggagatat 29

<210> 642

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 642

tagacacagg atgagaaaaa gggagatat 29

<210> 643

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 643

gcctttcttc ccgctcattc tc 22

<210> 644

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 644

gcctttcttc ccgctcattc tc 22

<210> 645

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 645

ccctacatcc atcttctgct actatg 26

<210> 646

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 646

ccctacatcc atcttctgct actatg 26

<210> 647

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 647

atgggctatt acatttttcc ttcatgga 28

<210> 648

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 648

atgggctatt acatttttcc ttcatgga 28

<210> 649

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 649

tacagcccat ttatagctca tcagttttt 29

<210> 650

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 650

tacagcccat ttatagctca tcagttttt 29

<210> 651

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 651

tagtacattg tttaacactt agcaagtgct 30

<210> 652

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 652

tagtacattg tttaacactt agcaagtgct 30

<210> 653

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 653

cttgaagtat aggcaagaca ggtgt 25

<210> 654

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 654

cttgaagtat aggcaagaca ggtgt 25

<210> 655

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 655

gaaaatgttg ccctttaaaa agcttcc 27

<210> 656

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 656

gaaaatgttg ccctttaaaa agcttcc 27

<210> 657

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 657

gtggaaattt ttattgcctc cgtattaca 29

<210> 658

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 658

gtggaaattt ttattgcctc cgtattaca 29

<210> 659

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 659

tgctctcaag gtgatagtga aataaaca 28

<210> 660

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 660

tgctctcaag gtgatagtga aataaaca 28

<210> 661

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 661

ctcatgtagt tcaagggcta acgt 24

<210> 662

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 662

ctcatgtagt tcaagggcta acgt 24

<210> 663

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 663

ctgagtttct gttactcttc cacgaa 26

<210> 664

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 664

ctgagtttct gttactcttc cacgaa 26

<210> 665

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 665

cataatcatg agctgaacac gaaatgg 27

<210> 666

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 666

cataatcatg agctgaacac gaaatgg 27

<210> 667

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 667

agttattgtg ctttccaact ccagatatt 29

<210> 668

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 668

agttattgtg ctttccaact ccagatatt 29

<210> 669

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 669

gatcaacctc ctaccatgtt acttcc 26

<210> 670

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 670

gatcaacctc ctaccatgtt acttcc 26

<210> 671

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 671

gaagctctgt gttgttcttt ccttg 25

<210> 672

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 672

gaagctctgt gttgttcttt ccttg 25

<210> 673

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 673

tcattctgct acaaacacaa tacgattttg 30

<210> 674

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 674

tcattctgct acaaacacaa tacgattttg 30

<210> 675

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 675

acagaatagc attttgccta tactatttgc 30

<210> 676

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 676

acagaatagc attttgccta tactatttgc 30

<210> 677

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 677

gtactaaaag gactctcaat cccttaagac 30

<210> 678

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 678

gtactaaaag gactctcaat cccttaagac 30

<210> 679

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 679

cacacacact cccatgctga tatt 24

<210> 680

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 680

cacacacact cccatgctga tatt 24

<210> 681

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 681

tatggtttct ttacacaaca gagtgtttga 30

<210> 682

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 682

tatggtttct ttacacaaca gagtgtttga 30

<210> 683

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 683

acaagccagt aagtaaataa tgtggtgt 28

<210> 684

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 684

acaagccagt aagtaaataa tgtggtgt 28

<210> 685

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 685

aaacaaaacc atgcttttta tttctaacgg 30

<210> 686

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 686

aaacaaaacc atgcttttta tttctaacgg 30

<210> 687

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 687

acaagatcaa tcagctaacc atgca 25

<210> 688

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 688

acaagatcaa tcagctaacc atgca 25

<210> 689

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 689

atccattctc tctcctggct tagt 24

<210> 690

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 690

atccattctc tctcctggct tagt 24

<210> 691

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 691

tgtgtaaaga gagacagaca gagaca 26

<210> 692

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 692

tgtgtaaaga gagacagaca gagaca 26

<210> 693

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 693

atgttatgaa ttgcaggtat ggaaaacac 29

<210> 694

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 694

atgttatgaa ttgcaggtat ggaaaacac 29

<210> 695

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 695

cctagcaaag ggcaaaacag tt 22

<210> 696

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 696

cctagcaaag ggcaaaacag tt 22

<210> 697

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 697

tcagctaaag gcaagtctga actc 24

<210> 698

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 698

tcagctaaag gcaagtctga actc 24

<210> 699

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 699

acagcaattt cagtgtccca gat 23

<210> 700

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 700

acagcaattt cagtgtccca gat 23

<210> 701

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 701

aatattctct cagaaaagaa agttggctca 30

<210> 702

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 702

aatattctct cagaaaagaa agttggctca 30

<210> 703

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 703

gtgttattgg catggaaatt gtgc 24

<210> 704

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 704

gtgttattgg catggaaatt gtgc 24

<210> 705

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 705

gcagatccca gttctgatct cca 23

<210> 706

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 706

gcagatccca gttctgatct cca 23

<210> 707

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 707

aaaaatgcat ttgggaatca atgaaatcc 29

<210> 708

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 708

aaaaatgcat ttgggaatca atgaaatcc 29

<210> 709

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 709

gagatattgt tctctttgct tgatttgtgt 30

<210> 710

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 710

gagatattgt tctctttgct tgatttgtgt 30

<210> 711

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 711

cagtggcata gctctcggaa ta 22

<210> 712

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 712

cagtggcata gctctcggaa ta 22

<210> 713

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 713

cttattctgt gggtcaagga tttggat 27

<210> 714

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 714

cttattctgt gggtcaagga tttggat 27

<210> 715

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 715

cccagcacca tctcttctga at 22

<210> 716

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 716

cccagcacca tctcttctga at 22

<210> 717

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 717

caaggagtcc ttactgaggc ctat 24

<210> 718

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 718

caaggagtcc ttactgaggc ctat 24

<210> 719

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 719

aacagagcct ttttatactg aactgtgt 28

<210> 720

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 720

aacagagcct ttttatactg aactgtgt 28

<210> 721

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 721

ggcaaaagaa cgaagttttc attgttc 27

<210> 722

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 722

ggcaaaagaa cgaagttttc attgttc 27

<210> 723

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 723

aaatagcgta cataacattc agaacctcat 30

<210> 724

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 724

aaatagcgta cataacattc agaacctcat 30

<210> 725

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 725

ggctgatcac agtattataa atggattgga 30

<210> 726

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 726

ggctgatcac agtattataa atggattgga 30

<210> 727

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 727

gctaggattt ggcactgttc ct 22

<210> 728

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 728

gctaggattt ggcactgttc ct 22

<210> 729

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 729

gttccctttg acaaaaagag aaaactact 29

<210> 730

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 730

gttccctttg acaaaaagag aaaactact 29

<210> 731

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 731

ggagctcagg acaatatgcc at 22

<210> 732

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 732

ggagctcagg acaatatgcc at 22

<210> 733

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 733

cgtgctttat taaatcttca tgggcaa 27

<210> 734

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 734

cgtgctttat taaatcttca tgggcaa 27

<210> 735

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 735

atgttattta acttcatgac actgggaaga 30

<210> 736

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 736

atgttattta acttcatgac actgggaaga 30

<210> 737

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 737

ccctcaagaa gtatatgaat aagggaatga 30

<210> 738

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 738

ccctcaagaa gtatatgaat aagggaatga 30

<210> 739

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 739

atctttcttt ttctccaacc ctcagtt 27

<210> 740

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 740

atctttcttt ttctccaacc ctcagtt 27

<210> 741

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 741

tttttctgaa aggttagagc tcaatgaga 29

<210> 742

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 742

tttttctgaa aggttagagc tcaatgaga 29

<210> 743

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 743

aaccacatga catcaaaagc tatagttct 29

<210> 744

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 744

aaccacatga catcaaaagc tatagttct 29

<210> 745

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 745

gtcctagata ttctgaacca aagtggt 27

<210> 746

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 746

gtcctagata ttctgaacca aagtggt 27

<210> 747

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 747

tacaaagact caggatatgc catatacaga 30

<210> 748

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 748

tacaaagact caggatatgc catatacaga 30

<210> 749

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 749

ttgacgaaat agaagagatt tcagcca 27

<210> 750

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 750

ttgacgaaat agaagagatt tcagcca 27

<210> 751

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 751

tgcctttgtc taaatgtagg tgctt 25

<210> 752

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 752

tgcctttgtc taaatgtagg tgctt 25

<210> 753

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 753

cttccagtaa atctcagcta aaaactgc 28

<210> 754

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 754

cttccagtaa atctcagcta aaaactgc 28

<210> 755

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 755

aaaaaggatg tatcctacca tgatgtgt 28

<210> 756

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 756

aaaaaggatg tatcctacca tgatgtgt 28

<210> 757

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 757

gccaaaaagg aggaaatcat aaaagagc 28

<210> 758

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 758

gccaaaaagg aggaaatcat aaaagagc 28

<210> 759

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 759

tctttcaaca ctgagggcta ggta 24

<210> 760

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 760

tctttcaaca ctgagggcta ggta 24

<210> 761

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 761

taaattacaa agccaatcta accactcact 30

<210> 762

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 762

taaattacaa agccaatcta accactcact 30

<210> 763

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 763

aagatccttg agagtaagaa ctttttgct 29

<210> 764

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 764

aagatccttg agagtaagaa ctttttgct 29

<210> 765

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 765

attgtgttct aagtaagcca accctt 26

<210> 766

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 766

attgtgttct aagtaagcca accctt 26

<210> 767

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 767

cagccaaacc atatcagtta ttgaagg 27

<210> 768

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 768

cagccaaacc atatcagtta ttgaagg 27

<210> 769

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 769

ttacccgtgt aaaagaaact atcctttacc 30

<210> 770

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 770

ttacccgtgt aaaagaaact atcctttacc 30

<210> 771

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 771

cagagacatt atcaaggcca tatttttgg 29

<210> 772

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 772

cagagacatt atcaaggcca tatttttgg 29

<210> 773

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 773

gtagcgtttt tatgatatat gtcattggca 30

<210> 774

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 774

gtagcgtttt tatgatatat gtcattggca 30

<210> 775

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 775

tccaagtgag gtattgaaga ctttttcaa 29

<210> 776

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 776

tccaagtgag gtattgaaga ctttttcaa 29

<210> 777

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 777

ttacctcctt ctaggttttt gataggagt 29

<210> 778

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 778

ttacctcctt ctaggttttt gataggagt 29

<210> 779

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 779

cccagaccat acttgtggga aa 22

<210> 780

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 780

cccagaccat acttgtggga aa 22

<210> 781

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 781

cctgtaccag gtaagaggga cat 23

<210> 782

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 782

cctgtaccag gtaagaggga cat 23

<210> 783

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 783

tttgatggta ttccatgaaa tcatcaggt 29

<210> 784

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 784

tttgatggta ttccatgaaa tcatcaggt 29

<210> 785

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 785

gccaccaaag ataaaacagt tttgca 26

<210> 786

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 786

gccaccaaag ataaaacagt tttgca 26

<210> 787

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 787

ccagcccact gctttatttt caa 23

<210> 788

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 788

ccagcccact gctttatttt caa 23

<210> 789

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 789

ggtatattgg tgatcgagaa agaccaa 27

<210> 790

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 790

ggtatattgg tgatcgagaa agaccaa 27

<210> 791

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 791

tccctaaaaa cagcagttta ttcatgtca 29

<210> 792

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 792

tccctaaaaa cagcagttta ttcatgtca 29

<210> 793

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 793

gaagacttgt aaagtcaaat gtgatactgc 30

<210> 794

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 794

gaagacttgt aaagtcaaat gtgatactgc 30

<210> 795

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 795

gaactatact tttgccaaca cttttagct 29

<210> 796

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 796

gaactatact tttgccaaca cttttagct 29

<210> 797

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 797

aacatcaaca cctaaagtga cctctt 26

<210> 798

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 798

aacatcaaca cctaaagtga cctctt 26

<210> 799

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 799

cctaacctcc tttctaagca gca 23

<210> 800

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 800

cctaacctcc tttctaagca gca 23

<210> 801

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 801

tcctacatag aggcatgtaa gagtca 26

<210> 802

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 802

tcctacatag aggcatgtaa gagtca 26

<210> 803

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 803

tgcaggacaa aatcacctct atttgt 26

<210> 804

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 804

tgcaggacaa aatcacctct atttgt 26

<210> 805

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 805

atctcatatt tgtggtagag gagatttaca a 31

<210> 806

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 806

atctcatatt tgtggtagag gagatttaca a 31

<210> 807

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 807

catttttatt gaacatccat cgagttcgt 29

<210> 808

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 808

catttttatt gaacatccat cgagttcgt 29

<210> 809

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 809

tcaataattc acagagctaa ccaaaggt 28

<210> 810

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 810

tcaataattc acagagctaa ccaaaggt 28

<210> 811

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 811

tggtttgttg taaggagacg ctt 23

<210> 812

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 812

tggtttgttg taaggagacg ctt 23

<210> 813

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 813

ccaagctcag aaaccaacag taagt 25

<210> 814

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 814

ccaagctcag aaaccaacag taagt 25

<210> 815

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 815

tcttcttgct atgaataatg tcaatccga 29

<210> 816

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 816

tcttcttgct atgaataatg tcaatccga 29

<210> 817

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 817

tggatgtaat acctgttatt aatgccct 28

<210> 818

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 818

tggatgtaat acctgttatt aatgccct 28

<210> 819

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 819

tttctcagtt ttcatttctc tgaaggact 29

<210> 820

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 820

tttctcagtt ttcatttctc tgaaggact 29

<210> 821

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 821

cttttacaag acattagctg ttgaacagtg 30

<210> 822

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 822

cttttacaag acattagctg ttgaacagtg 30

<210> 823

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 823

atgctgagtc aggaaatgat atggg 25

<210> 824

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 824

atgctgagtc aggaaatgat atggg 25

<210> 825

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 825

cctatgggca acagttcagc ta 22

<210> 826

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 826

cctatgggca acagttcagc ta 22

<210> 827

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 827

acaactttag gaactcgact aaaagatgg 29

<210> 828

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 828

acaactttag gaactcgact aaaagatgg 29

<210> 829

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 829

tattattcta ccgtcttctg gcttctatca 30

<210> 830

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 830

tattattcta ccgtcttctg gcttctatca 30

<210> 831

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 831

gcattgacaa ttggtcaagt ataaagatgt 30

<210> 832

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 832

gcattgacaa ttggtcaagt ataaagatgt 30

<210> 833

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 833

agctgttatc tgacttttga attccca 27

<210> 834

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 834

agctgttatc tgacttttga attccca 27

<210> 835

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 835

tttcaatcca atacatcttc attggacct 29

<210> 836

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 836

tttcaatcca atacatcttc attggacct 29

<210> 837

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 837

tttttgcagg taataatgac tgactcaaa 29

<210> 838

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 838

tttttgcagg taataatgac tgactcaaa 29

<210> 839

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 839

gccacggtaa ggaggtgaaa tg 22

<210> 840

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 840

gccacggtaa ggaggtgaaa tg 22

<210> 841

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 841

cctttaacct tttataccag agtggtcaa 29

<210> 842

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 842

cctttaacct tttataccag agtggtcaa 29

<210> 843

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 843

taggtgaagc atctatgtag aacttagtga 30

<210> 844

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 844

taggtgaagc atctatgtag aacttagtga 30

<210> 845

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 845

tgaaagcaaa tttacacaca gacaaagtt 29

<210> 846

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 846

tgaaagcaaa tttacacaca gacaaagtt 29

<210> 847

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 847

ggtgaagcta caaaaatatt agagggact 29

<210> 848

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 848

ggtgaagcta caaaaatatt agagggact 29

<210> 849

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 849

tgtgtgaaaa tgatgggctt catact 26

<210> 850

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 850

tgtgtgaaaa tgatgggctt catact 26

<210> 851

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 851

tccaccacgc agtactagag at 22

<210> 852

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 852

tccaccacgc agtactagag at 22

<210> 853

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 853

gattcaatcg ttttggtggg tgaa 24

<210> 854

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 854

gattcaatcg ttttggtggg tgaa 24

<210> 855

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 855

tagaattctc agcttggagt agaaagagt 29

<210> 856

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 856

tagaattctc agcttggagt agaaagagt 29

<210> 857

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 857

gaaaacattt catttcagac acagctgt 28

<210> 858

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 858

gaaaacattt catttcagac acagctgt 28

<210> 859

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 859

ctaattatgt cagaagagcc tcctgat 27

<210> 860

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 860

ctaattatgt cagaagagcc tcctgat 27

<210> 861

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 861

ggcagtccta actttagttt aatctctgt 29

<210> 862

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 862

ggcagtccta actttagttt aatctctgt 29

<210> 863

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 863

aagagaataa tgccaagaca atttctcct 29

<210> 864

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 864

aagagaataa tgccaagaca atttctcct 29

<210> 865

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 865

acaaaaatcc attatgaggc agaaatgtg 29

<210> 866

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 866

acaaaaatcc attatgaggc agaaatgtg 29

<210> 867

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 867

agcagagtcg tctgtttaaa ccc 23

<210> 868

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 868

agcagagtcg tctgtttaaa ccc 23

<210> 869

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 869

actaatgtta gtgcctctaa gatgacaga 29

<210> 870

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 870

actaatgtta gtgcctctaa gatgacaga 29

<210> 871

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 871

ggatgtaagt aaagcccact atcagtc 27

<210> 872

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 872

ggatgtaagt aaagcccact atcagtc 27

<210> 873

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 873

tctgtccttt gtgaagagaa aatttgact 29

<210> 874

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 874

tctgtccttt gtgaagagaa aatttgact 29

<210> 875

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 875

acataaaacc taccaaatct gaactgtgt 29

<210> 876

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 876

acataaaacc taccaaatct gaactgtgt 29

<210> 877

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 877

aaggaaccga gccttattca cc 22

<210> 878

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 878

aaggaaccga gccttattca cc 22

<210> 879

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 879

actcacagac aataggcata caattga 27

<210> 880

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 880

actcacagac aataggcata caattga 27

<210> 881

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 881

agacaagcta agagagggaa ctgt 24

<210> 882

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 882

agacaagcta agagagggaa ctgt 24

<210> 883

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 883

gacacacttg gagtatatag cactattcc 29

<210> 884

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 884

gacacacttg gagtatatag cactattcc 29

<210> 885

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 885

ggtcaggcag cttctagttg tt 22

<210> 886

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 886

ggtcaggcag cttctagttg tt 22

<210> 887

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 887

tcgtttaatc agtatgaatt caacttggga 30

<210> 888

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 888

tcgtttaatc agtatgaatt caacttggga 30

<210> 889

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 889

tgtatatgtt tattagcttg aagcattcca ca 32

<210> 890

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 890

tgtatatgtt tattagcttg aagcattcca ca 32

<210> 891

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 891

tacgcagcac aattggcttt tg 22

<210> 892

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 892

tacgcagcac aattggcttt tg 22

<210> 893

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 893

ccactttcct tcaggaacat gga 23

<210> 894

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 894

ccactttcct tcaggaacat gga 23

<210> 895

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 895

acaataacta aaagggaaat cccatgca 28

<210> 896

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 896

acaataacta aaagggaaat cccatgca 28

<210> 897

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 897

cactgtggac ctggatttcc aa 22

<210> 898

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 898

cactgtggac ctggatttcc aa 22

<210> 899

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 899

agtcaaatat attctgacat gctccaaaat a 31

<210> 900

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 900

agtcaaatat attctgacat gctccaaaat a 31

<210> 901

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 901

gctcagttca cagttgtgtc ca 22

<210> 902

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 902

gctcagttca cagttgtgtc ca 22

<210> 903

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 903

cttccctgaa gagattcata tgacatca 28

<210> 904

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 904

cttccctgaa gagattcata tgacatca 28

<210> 905

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 905

ttttcattgc tctgtaatat cccatcatga 30

<210> 906

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 906

ttttcattgc tctgtaatat cccatcatga 30

<210> 907

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 907

tgagacatca agttaaaaag cttctacaca 30

<210> 908

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 908

tgagacatca agttaaaaag cttctacaca 30

<210> 909

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 909

tacatagcac ttggcttatt ttccttatgt 30

<210> 910

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 910

tacatagcac ttggcttatt ttccttatgt 30

<210> 911

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 911

ctatctcgta aataagagct gggaagattg 30

<210> 912

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 912

ctatctcgta aataagagct gggaagattg 30

<210> 913

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 913

cagactcgtg tgaggtctag ga 22

<210> 914

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 914

cagactcgtg tgaggtctag ga 22

<210> 915

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 915

ccatatccaa tgataccacc atcctct 27

<210> 916

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 916

ccatatccaa tgataccacc atcctct 27

<210> 917

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 917

tacaatgtta cttaaacatg ccatcctca 29

<210> 918

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 918

tacaatgtta cttaaacatg ccatcctca 29

<210> 919

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 919

ctttttgttt ctttggtaag aaggcgaa 28

<210> 920

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 920

ctttttgttt ctttggtaag aaggcgaa 28

<210> 921

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 921

tcatagacaa ggaagggctc tctt 24

<210> 922

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 922

tcatagacaa ggaagggctc tctt 24

<210> 923

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 923

cagtgattac acccttaatc tttggc 26

<210> 924

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 924

cagtgattac acccttaatc tttggc 26

<210> 925

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 925

ggtcaaggtt aaaaggtttg gaatttactc 30

<210> 926

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 926

ggtcaaggtt aaaaggtttg gaatttactc 30

<210> 927

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 927

tgggaaatcc agttggaact gtg 23

<210> 928

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 928

tgggaaatcc agttggaact gtg 23

<210> 929

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 929

tagccaattt aagcaactgt aatccct 27

<210> 930

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 930

tagccaattt aagcaactgt aatccct 27

<210> 931

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 931

attgcaggag ttactttaat caccaca 27

<210> 932

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 932

attgcaggag ttactttaat caccaca 27

<210> 933

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 933

atttgtgaat tcaatagcat gactgcttac 30

<210> 934

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 934

atttgtgaat tcaatagcat gactgcttac 30

<210> 935

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 935

attctgattt gctgaatctt ttgttcaca 29

<210> 936

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 936

attctgattt gctgaatctt ttgttcaca 29

<210> 937

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 937

cttcatttta ctctggcttt caacgt 26

<210> 938

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 938

cttcatttta ctctggcttt caacgt 26

<210> 939

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 939

tttttagtag cctcctactg atgaatactg 30

<210> 940

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 940

tttttagtag cctcctactg atgaatactg 30

<210> 941

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 941

ctttctacct gcttaattgt atctgtgct 29

<210> 942

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 942

ctttctacct gcttaattgt atctgtgct 29

<210> 943

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 943

ggtgttctgc agcaggttaa gt 22

<210> 944

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 944

ggtgttctgc agcaggttaa gt 22

<210> 945

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 945

cccagaagtg tttttcctcc cta 23

<210> 946

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 946

cccagaagtg tttttcctcc cta 23

<210> 947

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 947

attgttattg atgtcactaa aaactgctca 30

<210> 948

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 948

attgttattg atgtcactaa aaactgctca 30

<210> 949

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 949

tgggatctca ctggtaaaga aaacaaag 28

<210> 950

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 950

tgggatctca ctggtaaaga aaacaaag 28

<210> 951

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 951

gtcatcattt taaagagctc tctcagtga 29

<210> 952

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 952

gtcatcattt taaagagctc tctcagtga 29

<210> 953

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 953

gcctttatct accatcagtt tcccat 26

<210> 954

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 954

gcctttatct accatcagtt tcccat 26

<210> 955

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 955

ctttcaacca cagaaaacaa acaacaaa 28

<210> 956

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 956

ctttcaacca cagaaaacaa acaacaaa 28

<210> 957

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 957

aagtacttgc attgattctc aactgga 27

<210> 958

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 958

aagtacttgc attgattctc aactgga 27

<210> 959

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 959

aaagaggaaa aagattcttt gcaagttctc 30

<210> 960

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 960

aaagaggaaa aagattcttt gcaagttctc 30

<210> 961

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 961

ttcataggag tttcgttgct ttagtcttt 29

<210> 962

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 962

ttcataggag tttcgttgct ttagtcttt 29

<210> 963

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 963

agattcagtc tgggagagct ctt 23

<210> 964

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 964

agattcagtc tgggagagct ctt 23

<210> 965

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 965

ggaggctgca atggaaaatg gta 23

<210> 966

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 966

ggaggctgca atggaaaatg gta 23

<210> 967

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 967

aaactgtaaa taagaagaca catgagctga 30

<210> 968

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 968

aaactgtaaa taagaagaca catgagctga 30

<210> 969

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 969

ttttatgggt tgcaaaatac ttctttgca 29

<210> 970

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 970

ttttatgggt tgcaaaatac ttctttgca 29

<210> 971

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 971

acagtaattg gcactgaaag aggt 24

<210> 972

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 972

acagtaattg gcactgaaag aggt 24

<210> 973

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 973

tcttaagttc catcctttgc aagtaagg 28

<210> 974

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 974

tcttaagttc catcctttgc aagtaagg 28

<210> 975

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 975

acgattttca tcctggaatg tatttaggt 29

<210> 976

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 976

acgattttca tcctggaatg tatttaggt 29

<210> 977

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 977

actgtgggat gttgagatga cac 23

<210> 978

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 978

actgtgggat gttgagatga cac 23

<210> 979

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 979

ggtaagggag actcctgctg aa 22

<210> 980

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 980

ggtaagggag actcctgctg aa 22

<210> 981

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 981

ggacactgca catggagttc tt 22

<210> 982

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 982

ggacactgca catggagttc tt 22

<210> 983

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 983

aaactaataa aaacgttggt gctaattgct 30

<210> 984

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 984

aaactaataa aaacgttggt gctaattgct 30

<210> 985

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 985

cggccttctt ccagaatttt tgg 23

<210> 986

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 986

cggccttctt ccagaatttt tgg 23

<210> 987

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 987

gcattgcatg acaaaaatct caattttctt 30

<210> 988

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 988

gcattgcatg acaaaaatct caattttctt 30

<210> 989

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 989

gcacacagta agcaccatta atgg 24

<210> 990

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 990

gcacacagta agcaccatta atgg 24

<210> 991

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 991

aaagatgcat gtttaccctc caact 25

<210> 992

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 992

aaagatgcat gtttaccctc caact 25

<210> 993

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 993

tgtcatccaa tgcacaaagc tttattatg 29

<210> 994

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 994

tgtcatccaa tgcacaaagc tttattatg 29

<210> 995

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 995

gcacctcagt tgcctcttta aac 23

<210> 996

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 996

gcacctcagt tgcctcttta aac 23

<210> 997

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 997

ggaccagtaa cgaagagctt ttga 24

<210> 998

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 998

ggaccagtaa cgaagagctt ttga 24

<210> 999

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 999

ggctatgccc tgggtgttaa ag 22

<210> 1000

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1000

ggctatgccc tgggtgttaa ag 22

<210> 1001

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1001

agattgattc ttcaaaaggc acaaactac 29

<210> 1002

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1002

agattgattc ttcaaaaggc acaaactac 29

<210> 1003

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1003

aaatctaaca agccatgtac ttacatggt 29

<210> 1004

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1004

aaatctaaca agccatgtac ttacatggt 29

<210> 1005

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1005

taggtggcat caatgtcatg tttaaca 27

<210> 1006

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1006

taggtggcat caatgtcatg tttaaca 27

<210> 1007

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1007

gggatggaga aaggccaaac tc 22

<210> 1008

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1008

gggatggaga aaggccaaac tc 22

<210> 1009

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1009

gccctagtgt cttcaagtca tgc 23

<210> 1010

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1010

gccctagtgt cttcaagtca tgc 23

<210> 1011

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1011

agaatgaaat gagtgaaaga ggaagct 27

<210> 1012

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1012

agaatgaaat gagtgaaaga ggaagct 27

<210> 1013

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1013

gtggtagttt gaactgtgag taactgt 27

<210> 1014

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1014

gtggtagttt gaactgtgag taactgt 27

<210> 1015

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1015

tgttggaggc ataaattggt acca 24

<210> 1016

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1016

tgttggaggc ataaattggt acca 24

<210> 1017

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1017

actgatgtgt ctgactattc ctgaga 26

<210> 1018

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1018

actgatgtgt ctgactattc ctgaga 26

<210> 1019

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1019

aaaaacaatt tcaacacagg aagctga 27

<210> 1020

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1020

aaaaacaatt tcaacacagg aagctga 27

<210> 1021

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1021

cccattgtgg gattcctccc ta 22

<210> 1022

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1022

cccattgtgg gattcctccc ta 22

<210> 1023

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1023

gcaaaagttc agatccgttt taaatgtca 29

<210> 1024

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1024

gcaaaagttc agatccgttt taaatgtca 29

<210> 1025

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1025

ctctgtgttt gcccaggtat ct 22

<210> 1026

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1026

ctctgtgttt gcccaggtat ct 22

<210> 1027

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1027

aaatgtctta ttcatggcca aactcatg 28

<210> 1028

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1028

aaatgtctta ttcatggcca aactcatg 28

<210> 1029

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1029

attgccaaga gatagacacc caattt 26

<210> 1030

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1030

attgccaaga gatagacacc caattt 26

<210> 1031

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1031

tggctatcct tagggagcta aaattaca 28

<210> 1032

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1032

tggctatcct tagggagcta aaattaca 28

<210> 1033

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1033

atgcccactt cactgactct tc 22

<210> 1034

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1034

atgcccactt cactgactct tc 22

<210> 1035

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1035

agaaataaag atcagaaagg cccattgta 29

<210> 1036

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1036

agaaataaag atcagaaagg cccattgta 29

<210> 1037

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1037

agggagttgg aagtcggaga tt 22

<210> 1038

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1038

agggagttgg aagtcggaga tt 22

<210> 1039

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1039

caaacaccta gatatccatc tgcctt 26

<210> 1040

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1040

caaacaccta gatatccatc tgcctt 26

<210> 1041

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1041

atgagtcctc ctaaggaata attgaacc 28

<210> 1042

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1042

atgagtcctc ctaaggaata attgaacc 28

<210> 1043

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1043

tatgatggca tactacacaa cactgaaaa 29

<210> 1044

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1044

tatgatggca tactacacaa cactgaaaa 29

<210> 1045

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1045

ggaagccagt catgttaggc at 22

<210> 1046

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1046

ggaagccagt catgttaggc at 22

<210> 1047

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1047

tatgggtggt tacaatttct gtttatggt 29

<210> 1048

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1048

tatgggtggt tacaatttct gtttatggt 29

<210> 1049

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1049

ctttggtcaa tgttttcttg aaggct 26

<210> 1050

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1050

tctaaacata cactgtgttg agagctaac 29

<210> 1051

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1051

gcaatatttt ggcagagtca tgca 24

<210> 1052

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1052

gggcttgaag aaggaacaga ttatgaaata 30

<210> 1053

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1053

cctcaggatc ttgttaaaac acaaacc 27

<210> 1054

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1054

tcatacctac tattacctgg cccaaa 26

<210> 1055

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1055

ctacagggaa gacagaagca agtt 24

<210> 1056

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1056

tgtattcacc tgtgttttga taaccttgt 29

<210> 1057

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1057

gtataaatcc tggcaaggga ttaaagaaaa 30

<210> 1058

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1058

aacatagttt tcagtgcaga gacaattttt 30

<210> 1059

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1059

tttgatgcta tgaataatgc tcccatga 28

<210> 1060

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1060

aggaatataa catttttgct ctgaagagct 30

<210> 1061

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1061

gctgtgcctg gctggattta ata 23

<210> 1062

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1062

tcatggtagg aaaggaagca aagac 25

<210> 1063

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1063

tgtccataat tggccaaaaa ttgca 25

<210> 1064

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1064

agaagcacgt tatgatcaac ctctt 25

<210> 1065

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1065

gcagatctgc cactgggtat at 22

<210> 1066

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1066

ggcctagaca ccctgaatct tc 22

<210> 1067

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1067

tttcttctta gaagtgttca gttggacaa 29

<210> 1068

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1068

aaaagggaga agagaaaagc ctgtt 25

<210> 1069

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1069

gggaggtaat atgaagaatc caactcct 28

<210> 1070

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1070

ctttgctcct tattagttcc acaaggt 27

<210> 1071

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1071

ggcagaaata attgggatcc catact 26

<210> 1072

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1072

gctacaatat tgctctatca ctgaaacttg 30

<210> 1073

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1073

agcctttcat attaccagac attttctcaa 30

<210> 1074

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1074

ctgggaccac tgctctaagt atatact 27

<210> 1075

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1075

gcatcttaaa gggcaggcag ata 23

<210> 1076

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1076

gtcaaatcgt catgttatac agctggtata 30

<210> 1077

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1077

atcaaaaatg atgaaatcat ctatgcagct 30

<210> 1078

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1078

tctgaaattc caagtaggat gtaccaac 28

<210> 1079

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1079

gggttatatt ctccatctcc caagtca 27

<210> 1080

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1080

gtaggctata atgcaaagca ttatggg 27

<210> 1081

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1081

cctgaagcat aaaatacaac agagagga 28

<210> 1082

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1082

ccataggaaa ctagtaacac aagcca 26

<210> 1083

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1083

atgaaagttt taacagggcc agtcta 26

<210> 1084

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1084

caatgctcaa tgaatactag ctggaaaaa 29

<210> 1085

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1085

aaaatcaccg gaagggttta cca 23

<210> 1086

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1086

tgtatttctt tcctccattt tgggatgt 28

<210> 1087

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1087

gggcaaatga gccatattac agttg 25

<210> 1088

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1088

cttgacagaa catgatttca tgtgca 26

<210> 1089

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1089

taaagcagat gcaactgaag acca 24

<210> 1090

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1090

cgccctccat cttaaagcta tct 23

<210> 1091

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1091

ccttgtggaa aacactgcta tagaca 26

<210> 1092

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1092

ctacagttat agcagttact tgtttgggt 29

<210> 1093

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1093

gggccaaatt aaagagcagt tctt 24

<210> 1094

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1094

gccagaaatt tcctttttac gtataggatt 30

<210> 1095

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1095

ttctgggaca caggaacaat taaatga 27

<210> 1096

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1096

tgaacagaaa gatgccttga aggt 24

<210> 1097

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1097

gtgaatgtga gctttcctca tcaattt 27

<210> 1098

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1098

ctgtgctctg tttgcattta tagatcc 27

<210> 1099

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1099

gtgtctgcct gttatctaga agataaagg 29

<210> 1100

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1100

gttatgtagg tccatggtat ggtcaag 27

<210> 1101

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1101

aaaaactgaa gatctagcag tattgggt 28

<210> 1102

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1102

gacagataga tatggatcaa caacgaaact 30

<210> 1103

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1103

tctttgacgt gagattgaat taaggaaact 30

<210> 1104

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1104

ccttgagcat ttttgagaaa tgtgtgt 27

<210> 1105

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1105

ttcaaccaca tcaggttgca attttt 26

<210> 1106

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1106

gtaaggatag gaggcagtgg taatttc 27

<210> 1107

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1107

actgggtaga aaaatgcact gatga 25

<210> 1108

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1108

acaagatgtt gatgacaaaa ctcaatatgc 30

<210> 1109

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1109

cgtcatgttg ttttatctat cagtgca 27

<210> 1110

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1110

aaatagatgt tcttggccat aaagaaggt 29

<210> 1111

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1111

agcacacaat tgttatctca gagtttct 28

<210> 1112

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1112

agactgggag aatttctggc cta 23

<210> 1113

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1113

agttttacct ttccattctt caagttttgg 30

<210> 1114

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1114

ctttctcaag ttccattgtt tccaaaattc 30

<210> 1115

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1115

cttcctttgg atgatctcaa agggt 25

<210> 1116

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1116

gagctaacta tttgtagttg tggcagtata 30

<210> 1117

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1117

aagacagctt tgctgactca ca 22

<210> 1118

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1118

gggcagatca aaactcttct attgtaaaa 29

<210> 1119

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1119

tgtcagttgt tccttcttgg tttca 25

<210> 1120

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1120

agtattgtgt aagtacagac aactgattgg 30

<210> 1121

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1121

aaaggtgaaa ctggagtaaa cgtattca 28

<210> 1122

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1122

ggagggttct gatacagttg tatagtcata 30

<210> 1123

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1123

tcttcatcat ttgggaatga gtgaagt 27

<210> 1124

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1124

gggcaatttc atttctcagt attttctgt 29

<210> 1125

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1125

gtctcacaag gataaaatca gggtatca 28

<210> 1126

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1126

tggcttcaca aagaaaagtc agaga 25

<210> 1127

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1127

gtgtcacaga tgcaggtgct ta 22

<210> 1128

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1128

gtctgtcatc ttgactgaaa gaatttttgt 30

<210> 1129

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1129

cccaaagctc atattgaaat ttgattcca 29

<210> 1130

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1130

cctctgactg cttctactca ttgc 24

<210> 1131

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1131

ggaaatggat gaaagtttca attgggt 27

<210> 1132

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1132

gatacccact atgagtgtgt gttcattatt 30

<210> 1133

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1133

cgtgcaaagg ctgtggagaa ta 22

<210> 1134

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1134

ttgtagttga catgaagtca tctatgtgg 29

<210> 1135

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1135

gggaaaacta cccttagaaa gaattctgt 29

<210> 1136

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1136

ggacagttga ggcactcatc ttaa 24

<210> 1137

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1137

gagacataac ctcactagtt ttgtgtttt 29

<210> 1138

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1138

ctgtaggttc aaacctcaat tttgcc 26

<210> 1139

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1139

acacaatcag tttgtcagtt aattgcc 27

<210> 1140

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1140

gcacaaactc ttaatagtta ctaaggcaga 30

<210> 1141

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1141

cgccttaaga tatgtccact acaatactac 30

<210> 1142

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1142

gtgccactac aaaacaccct aca 23

<210> 1143

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1143

tctcataaat gagttcagtt gcagaaagt 29

<210> 1144

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1144

acccatgaca atattagtaa acatgggata 30

<210> 1145

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1145

atcctgttat ttaggtattt acatgggctt 30

<210> 1146

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1146

actactgtca tacaattttt aggtgggaaa 30

<210> 1147

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1147

cgaaaagtga cagggaatta tatgagga 28

<210> 1148

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1148

cttgcaggtt ctgcacatgt attttt 26

<210> 1149

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1149

aagaaagttt gttttaaagc tcagagcaa 29

<210> 1150

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1150

ctactgctaa ggaaaacaga atgagagaat 30

<210> 1151

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1151

tccatttctt tgatgatcaa tgatcgtga 29

<210> 1152

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1152

caagctatcc atctgatcag cgattaataa 30

<210> 1153

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1153

catacttctg agaagacaat atcatgcct 29

<210> 1154

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1154

cctctgactg ctctacgaac tttac 25

<210> 1155

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1155

ggcacaagag atacagctgc aa 22

<210> 1156

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1156

gcagcctgaa tttgcagtat tcaaatc 27

<210> 1157

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1157

ctcacgtttc cctaacaccc aa 22

<210> 1158

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1158

atcttccagg agcactccct aa 22

<210> 1159

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1159

attggttggt aaattatgct tagtctcca 29

<210> 1160

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1160

gaggtaaata cttcaccttc ctcctttg 28

<210> 1161

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1161

ttgacaagaa tgagagctta tgaaaatgc 29

<210> 1162

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1162

gcaatcttga aaggtaaaag agtaccatac 30

<210> 1163

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1163

gcaaaacgtc attgaaagtg ctga 24

<210> 1164

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1164

ggatatttcc caggagacat tttggg 26

<210> 1165

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1165

agctgaaagc ctgttccaca tt 22

<210> 1166

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1166

catgacctaa ggatcattgt tgtttaagag 30

<210> 1167

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1167

tccatcaact gaataaacac actatggtt 29

<210> 1168

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1168

ctaaaaagtt ctggtacctc tttgtagtca 30

<210> 1169

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1169

gctttactcc tgtgacagat cca 23

<210> 1170

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1170

ggagagaatg taactgttga accaatgtta 30

<210> 1171

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1171

tgcttctttt ctctatcagt gctttttagt 30

<210> 1172

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1172

agtactcagt tttctgtatt gctctgaaag 30

<210> 1173

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1173

gctggctaaa gactgcctaa gaa 23

<210> 1174

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1174

ggatgctgag atttccatag gga 23

<210> 1175

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1175

gatgacaaat ttgaagtctg aaaaggct 28

<210> 1176

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1176

gatgtcggga atcttaacag caaag 25

<210> 1177

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1177

gggactgatt ttgctgcatc taataag 27

<210> 1178

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1178

catttcttat tgtttctgtc tctctggaga 30

<210> 1179

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1179

ggctgttgtc tttttcttta gtgatttttc 30

<210> 1180

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1180

gccagatttg tgcttcaaat aagca 25

<210> 1181

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1181

ggtagaggcc cagaaatctg tatc 24

<210> 1182

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1182

aggaaagtct aagaagctat cacaattcag 30

<210> 1183

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1183

tgtagcttta tgctaactct tgaatctgg 29

<210> 1184

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1184

tgcaagattc actgatgtaa aaatgtcaat 30

<210> 1185

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1185

tcttgcccta ttattctagg tagtacttcc 30

<210> 1186

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1186

ggagcttcct taacctaata ggagatacaa 30

<210> 1187

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1187

tgagtgattt ttgatacttt gcgtttttca 30

<210> 1188

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1188

ctgcacaaca tagactttat ctctacagaa 30

<210> 1189

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1189

ccctagcatt taaggtggca ttc 23

<210> 1190

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1190

gatacatttt atgtgcctcc tccaattttt 30

<210> 1191

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1191

gggcactgac agcatgtact aa 22

<210> 1192

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1192

gaagctgtcc ctctgtttct taattca 27

<210> 1193

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1193

ctcattaata tctagggcag aagaacca 28

<210> 1194

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1194

gggaaaagtt aattctgtct gagagtaagg 30

<210> 1195

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1195

tcataatagc agccattcta ttcatcatcc 30

<210> 1196

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1196

gaataaaagg gctgtgaacc ctttt 25

<210> 1197

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1197

taacccataa tgttgccatg agaaca 26

<210> 1198

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1198

taaacctgga ctaatctgat tgtagtacca 30

<210> 1199

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1199

ggtccagata tgattaggat gaggcta 27

<210> 1200

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1200

ctcattcctt gtgcagaact tcct 24

<210> 1201

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1201

tggtgtccct gggttcattt tt 22

<210> 1202

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1202

tcttctcagt gatagctgat gagga 25

<210> 1203

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1203

tgatacctgc ctcttctctt gct 23

<210> 1204

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1204

tgagcatgac agtgtcatgc tt 22

<210> 1205

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1205

tctgtgggtt tagcagagta caaag 25

<210> 1206

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1206

caacttcagt caaaatgtgg accaaaataa 30

<210> 1207

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1207

cgttatttcc aagagattct agggcaa 27

<210> 1208

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1208

caggaagttg ataacataga tgtgagtgt 29

<210> 1209

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1209

cagtcctctg caagaaccat tttaaaa 27

<210> 1210

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1210

ataataggtt ccacacataa aagcagct 28

<210> 1211

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1211

cctcagacct attttacctc tcctgaa 27

<210> 1212

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1212

cacagaaatc actgttcacg aatgag 26

<210> 1213

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1213

gctcccaaca acagccttgt aa 22

<210> 1214

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1214

agtgtggcca caatatggaa tgag 24

<210> 1215

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1215

acaacgtcct ggtgttcata ctc 23

<210> 1216

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1216

ctcacttcct tgagatgctc acaa 24

<210> 1217

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1217

tgttaagtgc ctctgtgcga tag 23

<210> 1218

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1218

gggtcagttg ttcttgttta atcaaaaac 29

<210> 1219

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1219

cagagatctt atgactcaca atgcct 26

<210> 1220

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1220

agggtgctaa ctgacaccta gt 22

<210> 1221

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1221

cctgagaaat caagagatat tttcggga 28

<210> 1222

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1222

gagtctttca gcaagataga ctttacaatc 30

<210> 1223

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1223

ctctttttaa tgtgatctgg gatagatcca 30

<210> 1224

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1224

ccatccctct ttgactcaaa gaaatgtata 30

<210> 1225

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1225

gcagaagaca cataccagtg ttga 24

<210> 1226

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1226

ctaccttttt ccgcttgctc att 23

<210> 1227

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1227

gtggatcctc cagtctcaat cag 23

<210> 1228

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1228

cacttgagtg aacatagcga caaaatag 28

<210> 1229

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1229

ccagacatct gtccagatgc at 22

<210> 1230

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1230

cagtttgcat acagtgagat taaaaagcat 30

<210> 1231

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1231

gttaaggaaa ccaaggttga aagaagtg 28

<210> 1232

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1232

ttgctatggc ttcactgatt atcaaaga 28

<210> 1233

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1233

acctccaggt cccttgaact ta 22

<210> 1234

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1234

aaagaaataa tgcaggtcag aggtgat 27

<210> 1235

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1235

ggtgcctcta attaatggtt gtgaatt 27

<210> 1236

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1236

cagtgaatac aatctttcat tttctggtgt 30

<210> 1237

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1237

ccggtataga cccacagcat ct 22

<210> 1238

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1238

agcaggatac ctactccagt cttg 24

<210> 1239

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1239

gattctgaaa agggatccat aggca 25

<210> 1240

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1240

cccatttaat acatcagtcc cacataca 28

<210> 1241

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1241

cccagaaaga aggtgtcaag attact 26

<210> 1242

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1242

ggctgcctca tatctagcag aag 23

<210> 1243

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1243

gcagtctcta gagcattctt agca 24

<210> 1244

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1244

agtgagaaga cctgggtgtt ct 22

<210> 1245

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1245

ctggagagca attctgggaa aca 23

<210> 1246

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1246

tcctacagta cttaacattt ctgtgtatgc 30

Claims (3)

1.一种引物组合物，其特征在于，包括能够特异性扩增杜氏肌营养不良基因编码区位点和目标SNP位点的多个引物对，所述多个引物对的核苷酸序列分别如SEQ ID NO.1～SEQID NO.1246所示。

2.一种用于检测杜氏肌营养不良基因缺陷的试剂盒，其特征在于，包括权利要求1所述的引物组合物。

3.根据权利要求2所述的试剂盒，其特征在于，还包括通用引物、多重PCR聚合酶、DNA连接酶、末端修复酶、dNTPs和反应缓冲液。