CN111527395A - 一种免疫细胞中淋巴细胞的流式细胞仪检测方法 - Google Patents
一种免疫细胞中淋巴细胞的流式细胞仪检测方法 Download PDFInfo
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Abstract
提供一种免疫细胞中淋巴细胞的流式细胞仪检测方法，该方法包括：a)取免疫荧光抗体染色的淋巴细胞样品，加入预冷的PBS‑EDTA溶液，混均细胞，备流式细胞仪检测待用；b)对流式细胞仪系统进行开机预热，调节液流速度，然后向样品管中加入PBS‑EDTA溶液，冲洗液流的喷嘴系统；c)将步骤a)所得混均细胞样品加入样品管然后进行检测。该检测方法在检测时可使亚群分群明显，分析检测更加准确，有利于检测数量较少的细胞亚群，并节省抗体试剂。
Description
PCT国内申请，说明书已公开。
Claims (19)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2018/118802 WO2020107496A1 (zh) | 2018-12-01 | 2018-12-01 | 一种免疫细胞中淋巴细胞的流式细胞仪检测方法 |
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| Publication Number | Publication Date |
|---|---|
| CN111527395A true CN111527395A (zh) | 2020-08-11 |
| CN111527395B CN111527395B (zh) | 2024-01-26 |
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| CN201880002857.3A Active CN111527395B (zh) | 2018-12-01 | 2018-12-01 | 一种免疫细胞中淋巴细胞的流式细胞仪检测方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20210389225A1 (zh) |
| EP (1) | EP3889576A4 (zh) |
| CN (1) | CN111527395B (zh) |
| WO (1) | WO2020107496A1 (zh) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112268849A (zh) * | 2020-10-30 | 2021-01-26 | 暨南大学 | 一种健康人免疫功能评估分析方法 |
| CN113917159A (zh) * | 2021-09-30 | 2022-01-11 | 杭州联科生物技术股份有限公司 | 一种淋巴细胞亚群的流式检测方法 |
| CN114410580A (zh) * | 2022-02-07 | 2022-04-29 | 无锡观合医学检验所有限公司 | 一种pbmc的提取方法 |
| CN114720358A (zh) * | 2022-04-11 | 2022-07-08 | 浙江普罗亭健康科技有限公司 | 一种质谱流式血液肿瘤免疫分型中替代侧向散射光信号的抗体组合及应用 |
| WO2023115703A1 (zh) * | 2021-12-22 | 2023-06-29 | 重庆医科大学附属儿童医院 | 一种b淋巴细胞发育亚群免疫分型的方法和试剂盒 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114578048B (zh) * | 2021-12-22 | 2023-08-08 | 重庆医科大学附属儿童医院 | 一种t淋巴细胞发育亚群免疫分型的方法和试剂盒 |
| CN117165521A (zh) * | 2023-02-09 | 2023-12-05 | 昭衍(北京)检测技术有限公司 | 用于分离及体外获得外周血单个核细胞(pbmc)的方法 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005124350A1 (en) * | 2004-06-21 | 2005-12-29 | Fondazione Santa Lucia I.R.C.C.S. | Use of multiparametric flow cytometry for the diagnosis, prognosis, and validation of immunotherapies in autoimmune, hematologic, and lymphoproliferative diseases |
| US20090258347A1 (en) * | 2004-12-14 | 2009-10-15 | University Of The Witwatersrand | Method for diagnosing and monitoring cellular reservoirs of disease |
| CN103760349A (zh) * | 2013-12-24 | 2014-04-30 | 郑州大学第一附属医院 | 一种鉴定恶性胸腔积液的方法 |
| CN104133058A (zh) * | 2014-07-31 | 2014-11-05 | 北京海思特临床检验所有限公司 | 用于检测nk细胞活性的试剂盒 |
| CN106771242A (zh) * | 2016-12-16 | 2017-05-31 | 石河子大学 | 一种证明硫化氢与缝隙连接关系的实验方法 |
| CN107063982A (zh) * | 2017-05-09 | 2017-08-18 | 西华师范大学 | 流式细胞术检测鸡外周血t淋巴细胞亚群的方法 |
| CN107167415A (zh) * | 2017-05-09 | 2017-09-15 | 成都扬克斯生物科技有限公司 | 流式细胞术检测猪外周血t淋巴细胞亚群的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5087295A (en) * | 1990-06-13 | 1992-02-11 | Becton Dickinson And Company | Cleaning cycle for flow cytometers |
| EP1395826A4 (en) * | 2001-05-17 | 2005-11-09 | Miltenyi Biotec Gmbh | ANTIGEN-BINDING FRAGMENTS FOR A SUB-GROUP OF DENDRITIC CELLS AND USE THEREOF |
| IL161856A0 (en) * | 2001-11-30 | 2005-11-20 | Pfizer Prod Inc | Methods for detecting cells with numerical chromosomal abnormalities |
| JP6956632B2 (ja) * | 2014-06-06 | 2021-11-02 | ヘアレウ ホスピタル | Ｍｈｃ多量体のバーコード標識による抗原認識の判定 |
| CN104941001A (zh) * | 2015-07-10 | 2015-09-30 | 奥思达干细胞有限公司 | 一种生物膜角膜贴片及其制备方法和应用 |
| CN105547971B (zh) * | 2016-01-08 | 2018-05-01 | 首都医科大学附属北京友谊医院 | 细胞毒性t细胞脱颗粒的流式细胞术检测方法 |
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2018
- 2018-12-01 EP EP18941524.3A patent/EP3889576A4/en active Pending
- 2018-12-01 US US17/295,655 patent/US20210389225A1/en active Pending
- 2018-12-01 WO PCT/CN2018/118802 patent/WO2020107496A1/zh active Application Filing
- 2018-12-01 CN CN201880002857.3A patent/CN111527395B/zh active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005124350A1 (en) * | 2004-06-21 | 2005-12-29 | Fondazione Santa Lucia I.R.C.C.S. | Use of multiparametric flow cytometry for the diagnosis, prognosis, and validation of immunotherapies in autoimmune, hematologic, and lymphoproliferative diseases |
| US20090258347A1 (en) * | 2004-12-14 | 2009-10-15 | University Of The Witwatersrand | Method for diagnosing and monitoring cellular reservoirs of disease |
| CN103760349A (zh) * | 2013-12-24 | 2014-04-30 | 郑州大学第一附属医院 | 一种鉴定恶性胸腔积液的方法 |
| CN104133058A (zh) * | 2014-07-31 | 2014-11-05 | 北京海思特临床检验所有限公司 | 用于检测nk细胞活性的试剂盒 |
| CN106771242A (zh) * | 2016-12-16 | 2017-05-31 | 石河子大学 | 一种证明硫化氢与缝隙连接关系的实验方法 |
| CN107063982A (zh) * | 2017-05-09 | 2017-08-18 | 西华师范大学 | 流式细胞术检测鸡外周血t淋巴细胞亚群的方法 |
| CN107167415A (zh) * | 2017-05-09 | 2017-09-15 | 成都扬克斯生物科技有限公司 | 流式细胞术检测猪外周血t淋巴细胞亚群的方法 |
Non-Patent Citations (1)
| Title |
|---|
| 张均田 等: "《现代药理实验方法（上册）》", 中国协和医科大学出版社, pages: 683 - 684 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112268849A (zh) * | 2020-10-30 | 2021-01-26 | 暨南大学 | 一种健康人免疫功能评估分析方法 |
| CN113917159A (zh) * | 2021-09-30 | 2022-01-11 | 杭州联科生物技术股份有限公司 | 一种淋巴细胞亚群的流式检测方法 |
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| CN114720358B (zh) * | 2022-04-11 | 2022-09-27 | 浙江普罗亭健康科技有限公司 | 一种质谱流式血液肿瘤免疫分型中替代侧向散射光信号的抗体组合及应用 |
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| EP3889576A1 (en) | 2021-10-06 |
| EP3889576A4 (en) | 2022-09-07 |
| WO2020107496A1 (zh) | 2020-06-04 |
| US20210389225A1 (en) | 2021-12-16 |
| CN111527395B (zh) | 2024-01-26 |
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