CN105547971B - 细胞毒性t细胞脱颗粒的流式细胞术检测方法 - Google Patents
细胞毒性t细胞脱颗粒的流式细胞术检测方法 Download PDFInfo
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- CN105547971B CN105547971B CN201610013621.2A CN201610013621A CN105547971B CN 105547971 B CN105547971 B CN 105547971B CN 201610013621 A CN201610013621 A CN 201610013621A CN 105547971 B CN105547971 B CN 105547971B
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- 238000001514 detection method Methods 0.000 claims abstract description 22
- 230000001472 cytotoxic effect Effects 0.000 claims abstract description 7
- 238000011534 incubation Methods 0.000 claims abstract description 7
- 102100035133 Lysosome-associated membrane glycoprotein 1 Human genes 0.000 claims abstract description 6
- 231100000433 cytotoxic Toxicity 0.000 claims abstract description 6
- 230000005284 excitation Effects 0.000 claims abstract description 5
- 108010052285 Membrane Proteins Proteins 0.000 claims abstract description 4
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1481—Optical analysis of particles within droplets
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- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
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CN201610013621.2A CN105547971B (zh) | 2016-01-08 | 2016-01-08 | 细胞毒性t细胞脱颗粒的流式细胞术检测方法 |
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CN201610013621.2A CN105547971B (zh) | 2016-01-08 | 2016-01-08 | 细胞毒性t细胞脱颗粒的流式细胞术检测方法 |
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CN105547971A CN105547971A (zh) | 2016-05-04 |
CN105547971B true CN105547971B (zh) | 2018-05-01 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108226469B (zh) * | 2018-01-05 | 2020-07-31 | 首都医科大学附属北京友谊医院 | 一种快速诊断家族性噬血细胞综合征3型的试剂盒及其应用 |
CN111527395B (zh) * | 2018-12-01 | 2024-01-26 | 铭道创新(北京)医疗技术有限公司 | 一种免疫细胞中淋巴细胞的流式细胞仪检测方法 |
CN110320352A (zh) * | 2019-07-25 | 2019-10-11 | 上海轩锋生物科技有限公司 | 基于抗体依赖的细胞介导细胞毒性的抗体效价检测方法 |
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CN1245628C (zh) * | 2001-05-22 | 2006-03-15 | 中国医学科学院基础医学研究所 | 检测外周血抗原特异性细胞毒性t淋巴细胞的方法及试剂盒 |
KR20070001930A (ko) * | 2004-01-23 | 2007-01-04 | 사노피 파스퇴르 인크 | 세포독성 분석 |
WO2005100553A2 (en) * | 2004-04-16 | 2005-10-27 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Method for assessment of cytotoxic lymphocyte activity |
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2016
- 2016-01-08 CN CN201610013621.2A patent/CN105547971B/zh active Active
Non-Patent Citations (3)
Title |
---|
Comparison of RBL-2H3 and P815 Cell Lines for Establishing in vitro Model of Mast Cell Degranulation;PENG Bo,et al;《中国天然药物》;20110531;第9卷(第3期);227-231 * |
基于流式细胞术评价单核细胞介导的抗体依赖性细胞介导的细胞毒效应的方法学建立和应用;梁华,等;《中华微生物学和免疫学杂志》;20150131;第35卷(第1期);18-22 * |
树突状细胞与肥大细胞瘤细胞融合后诱导T细胞介导的抗肿瘤效应;王全楚,等;《中华肝脏病杂志》;20040930;第12卷(第9期);559-560 * |
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