CN111492877B - Fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries - Google Patents

Fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries Download PDF

Info

Publication number
CN111492877B
CN111492877B CN202010306434.XA CN202010306434A CN111492877B CN 111492877 B CN111492877 B CN 111492877B CN 202010306434 A CN202010306434 A CN 202010306434A CN 111492877 B CN111492877 B CN 111492877B
Authority
CN
China
Prior art keywords
mulberry
planting
months
branches
year
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN202010306434.XA
Other languages
Chinese (zh)
Other versions
CN111492877A (en
Inventor
胡桂萍
叶川
彭晓虹
邓真华
石旭平
王亚威
蔡翔
曹红妹
黄金枝
杜贤明
俞燕芳
王丰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangxi Sericulture Tea Research Institute (jiangxi Economic Crop Research Institute)
Original Assignee
Jiangxi Sericulture Tea Research Institute (jiangxi Economic Crop Research Institute)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangxi Sericulture Tea Research Institute (jiangxi Economic Crop Research Institute) filed Critical Jiangxi Sericulture Tea Research Institute (jiangxi Economic Crop Research Institute)
Priority to CN202010306434.XA priority Critical patent/CN111492877B/en
Publication of CN111492877A publication Critical patent/CN111492877A/en
Application granted granted Critical
Publication of CN111492877B publication Critical patent/CN111492877B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00—Methods for working soil
    • A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
    • A01G13/0256—Ground coverings
    • A01G13/0268—Mats or sheets, e.g. nets or fabrics
    • A01G13/0275—Films
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Forests & Forestry (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Virology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Mechanical Engineering (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries, and belongs to the technical field of agricultural cultivation. The invention discloses a fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries, which comprises the following steps: 1) preparing and cleaning planting soil; 2) selecting mulberry seedlings; 3) planting time; 4) planting density; 5) planting method; 6) cultivating tree forms; 7) enhancing the tree vigor; 8) and (4) preventing and controlling plant diseases and insect pests. The mulberry fruit obtained by the method has good shape, bright color, rich nutrition, safety and high quality, the quality of the mulberry fruit reaches the A-grade product standard of green food, and the pesticide residue of the sanitary standard is zero and exceeds the standard.

Description

Fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries
Technical Field
The invention relates to the technical field of agricultural cultivation, in particular to a fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries.
Background
Fruit mulberry is a general name for a type of mulberry used to produce mulberry (also known as mulberry). Modern medical and pharmaceutical researches show that the mulberry is rich in nutrition, contains various vitamins, organic acids, free amino acids and trace elements, also contains rich anthocyanin, resveratrol and other bioactive components and trace element selenium, has good effects of resisting oxidation, aging and cancers, resisting viruses and ulcer and the like, is known as the best health-care fruit product in the 21 st century, and is one of the first medical and edible agricultural products in the health department. The mulberry is mainly eaten by fresh fruits or processed into food, beverage and the like. However, the fruit mulberry has various diseases and insect pests, is common in occurrence and serious in damage, and according to incomplete statistics, at least 23 kinds of insect pests and 9 kinds of diseases can be damaged and damaged during the period from the germination period to the picking period of the fruit mulberry, so that the yield and the quality of the mulberry are influenced.
Modern production practices for preventing and treating diseases and insect pests of mulberries show that chemical prevention and treatment are mainly performed, the used pesticides are various and mixed, the pesticide application frequency is more frequent, and the pesticide application concentration is higher. For example, more than 20 varieties of available pesticides can be produced for preventing and treating the sclerotinia rot of the mulberry at present, and the varieties of the pesticides with good preventing and treating effects comprise thiophanate methyl, carbendazim, dimethachlon, procymidone, mancozeb, tebuconazole and the like; the pesticide application period is as long as 65-50 days, namely 15 days from the initial flowering stage of the mulberry to before mulberry picking; the pesticide is used for several times and dosage on average. However, most of the pesticides with good control effects are not in a list of pesticide varieties allowed to be used by green foods, and the mulberries are extremely thin berries with the epicarp, and the pesticide enrichment and absorption capacity of the mulberries is several times to dozens of times higher than that of berries such as leathery peels, so that the potential safety hazard of the mulberry pesticides is obvious, and the pressure for meeting the requirements of national green healthy and safe foods is great. Therefore, the pesticide residue in the mulberry is effectively reduced, the quality safety level of the mulberry is improved, and the method has great significance for improving the green and healthy development of the mulberry industry.
The conventional mode for reducing pesticide residues of vegetables and fruits is to change a pest prevention and control strategy, and reduce chemical pesticides by using a pest green prevention and control technology, such as physical prevention and control of yellow boards, black light lamps and the like, microbial pesticides, natural enemy insects of botanical pesticides, chemical pesticide substitution technology and the like, so that zero overproof pesticide residues are achieved. However, the data show that the factors causing pesticide residues on vegetables and fruits have the characteristics of complexity and diversification, for example, the soil cleanness and the quality level of a planting field influence the source pesticide safety of the vegetables and fruits, the disease and insect resistance of varieties of the vegetables and fruits determines the disease degree and the pesticide use condition of trees, the growth trend of crops is influenced by the cultivation technology to further influence the pest damage condition, and the pest control strategy determines the green safety level of the crops. Therefore, for pesticide residue control, various factors such as crop varieties, environmental quality, cultivation management and the like need to be comprehensively considered, and the green safety of the fruits and vegetables is systematically, scientifically and accurately ensured.
Therefore, the problem to be solved by the technical personnel in the field is to provide a fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries.
Disclosure of Invention
In view of the above, in order to solve the problems of obvious potential safety hazard of pesticides and missing of green safe production technology in mulberry production, the invention provides a mulberry green cultivation method for effectively reducing pesticide residue of mulberries by practice, improvement and improvement from the aspects of preparation and cleaning of planting lands, selection of mulberry varieties, planting methods, tree formation, tree vigor enhancement and prevention and control of diseases, insect pests and weeds based on the growth period characteristics of mulberries by taking the pesticide residue cause of mulberries as an entry point and combining with the pesticide residue prevention and control key risk points.
In order to achieve the purpose, the invention adopts the following technical scheme:
a fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries is characterized by comprising the following specific steps:
(1) preparing and cleaning planting soil:
selecting a loam plot with flat terrain, convenient irrigation and drainage, ventilation and sunny exposure and sterile nuclear disease, wherein the thickness of a soil layer is more than 50cm, and the pH value is 5.0-7.5; 15-20 days before planting, applying 35-40 kg of microbial contamination reducing agent and 32.5-35 kg of tea seed shells to each mu of planting land, and ploughing to a depth of 60-80 cm to clean soil of the planting land;
(2) selecting mulberry seedlings:
selecting excellent hybrid mulberry varieties with strong disease resistance, waterlogging tolerance, close planting and low dryness, wherein mulberry seedlings are uniform in size, the diameter of the seedlings is 10.0-15.0mm, the main roots are complete, and the root length is not less than 130 mm;
(3) planting time:
in the middle and last ten days of 2 months or from the bottom of 11 months to the beginning of 12 months every year;
(4) planting density:
planting 70-100 plants per mu, wherein the plant spacing is 2.0-2.5 m, and the row spacing is 3.0-4.0 m;
(5) the planting method comprises the following steps:
before planting, trimming off the tender and weak part of the fruit mulberry seedling with the length of 60-70 cm, and cutting the root system for 20 cm; hole planting is adopted, and the planting hole size is 50cm multiplied by 50 cm; before planting, applying 3.0 kg-5.0 kg of decomposed organic fertilizer in each planting hole, covering soil after applying the fertilizer and treading the soil to be 5cm-10cm lower than the ground; spreading the root system of the seedling, placing the seedling in the center of a planting hole, strengthening the body, filling soil, slightly lifting, treading, and thoroughly watering the root fixing water;
(6) tree-shaped cultivation:
after field planting, drying the mulberry leaves 40-50 cm away from the ground; thinning buds when the young shoots on the trunk grow to 10-15 cm, selecting and reserving 2-3 healthy young shoots which are uniformly distributed at the rooting positions, and culturing the young shoots into thick and strong branches;
before the end of 3 months next year, primary branch stems are fixed at the positions of fruit mulberry which are 70 cm-80 cm away from the ground; after sprouting, when young shoots grow to 10-15 cm, thinning the sprouts, selecting and reserving 2-3 young shoots on each branch, and pinching when the young shoots grow to 90-100 cm away from the ground to form 3-6 secondary branches; after sprouting, 2-3 new shoots are reserved on each branch, namely the fruit bearing branches in the next year;
cutting a fixed fist at the position of 90-100 cm away from the ground after picking fruits in the third year; after sprouting, keeping 2-4 young shoots on each fist, and cultivating into fruit bearing branches; cutting at mulberry fist every year;
(7) enhancement of tree vigor
Fertilizing:
when mulberry buds sprout, 0.2 kg-0.5 kg of organic-inorganic compound fertilizer N, P, K is 5, 3, 4 (organic matter is more than 20 percent) is applied to each mulberry bud; after harvesting and cutting mulberry, 0.2 kg-0.5 kg of organic-inorganic compound fertilizer N, P, K and 2.0 kg-3.0 kg of organic fertilizer (organic matter is more than or equal to 40 percent) are applied to each mulberry; after mulberry leaves fall, applying organic fertilizer again, wherein 4.0 kg-5.0 kg of organic fertilizer is applied to each mulberry; during fertilization, ditching and fertilizing near the water dripping line of the crown, and covering soil after fertilization;
and II, trimming:
cutting annual fruit hanging branches at a mulberry fist after the mulberry harvesting work is completed in 5 months every year; when the buds on a mulberry fist germinate, thinning the buds with improper implantation positions, excessive density, weakness, over-strength and diseases and pests, controlling the number of branches for bearing fruits of a single plant to be 25-30, exposing the cut branches and leaves at a high temperature of more than 30 ℃ for more than 7 days, mechanically crushing the branches and leaves into particles with the size of 1.3-1.8cm multiplied by 2.1-2.5cm, returning a root rotating machine to a garden, and rotating the roots to the depth of 20-25 cm; after the mulberry leaves fall and before mulberry buds sprout at the end of 11 months every year to the beginning of 2 months next year, pruning dead branches, insect-plague branches, weak twigs, lateral lying branches and dead fists, removing the prunes out of the garden, and adding lime water for centralized treatment;
injecting nutrient solution:
injecting 1000ml of tree vigor enhancing nutrient solution every 15 days from the beginning of flowering to the olive period every year;
(8) prevention and control of plant diseases and insect pests
S1, ground fabric use and cover: covering black single-side silty ground cloth on the mulberry orchard from 1 ten days to 2 middle days of each year according to the ratio of 185g/m2Performing full-circle coverage according to the standard; at the end of 5 months, after the mulberry is harvested, the ground fabric is timely collected, and the mulberry is cleaned, aired and stored for recycling, and before next use, 30% of bactericide is sprayed 7 days in advance, sterilized and naturally aired for use;
s2, controlling the mulberry psylla: carrying out mulberry field mulberry psylla prediction and forecast from the end of 2 months to the beginning of the third month every year, namely when mulberry sprouts, releasing Paederia pteris or trichogramma when the number of ten-leaf insects in the field of the mulberry psylla exceeds 4, and releasing according to the bee-insect release ratio of 8:1 per mu;
s3, preventing and treating sclerotinia rot: spraying liquid which is 800-1000 times of 50% procymidone or liquid which is 800-1000 times of 70% thiophanate methyl in time from the beginning of flowering period to the middle and upper ten days of 4 months each year when the sclerotinia rot of the mulberry appears from the beginning of flowering period to the young fruit period, alternately using the two solutions, spraying the two solutions once in 5-6 days, and stopping using the two solutions 25 days before harvesting;
s4, mulberry moth prevention and control: at the beginning of 5 months to 9 months every year, carrying out the field prediction of the mulberry borers; when the population density of the mulberry borers in the field is below 5 per plant, suspending sex pheromone for trapping, and installing 2 in each mu; when the population density of the mulberry borer is 5-15 per plant, 50-60 ml of metarhizium anisopliae (metarhizium anisopliae CQMa117 microgranule, Chongqing major biotechnology development Co., Ltd.) is sprayed per mu, and the metarhizium anisopliae is diluted by 1000-1200 times for prevention and treatment, wherein the concentration of the metarhizium anisopliae before dilution is 10-12 hundred million spores/ml; pruning mulberry trees in summer when the population density of the mulberry borers exceeds 30 per mulberry, spraying metarhizium anisopliae to the pruned branches in a centralized manner, and spraying 5kg of film to each ton of pruned branches and sealing for 72 hours;
s5, prevention and treatment of the mulberry longicorn: in the middle and late 6 months to 8 months, the longicorn is prevented and controlled by adopting a bait agent, the bait agent is suspended at a position 0.7m above the ground in a canning manner, and is replaced every 2 weeks;
s6, mulberry field weed treatment: adopts a mode of treating grass by grass, and the grass seeds are as follows: the sowing amount per mu of the white clover, the oxtail grass and the ryegrass is as follows: 0.5-0.8 kg: 0.2-0.5 kg: 0.3-0.5kg, the sowing mode is mixed drill sowing, the drill sowing depth is 20cm, and the sowing time is 3 months per year.
Further, the microorganism pollution-reducing agent in the step (1) comprises the following specific components: 30-35% of pseudomonas fermentation liquor, 10-12% of acinetobacter fermentation liquor, 15-18% of bacillus fermentation liquor, 3-5% of uric acid, 0-0.5% of tween 800.3, 0.2-0.3% of boric acid, 0.5-0.8% of dipotassium phosphate, 0.5-0.6% of citric acid, 0.2-0.25% of copper sulfate, 0.3-0.35% of ferrous sulfate and 29-40% of water.
Pseudomonas, Acinetobacter and Bacillus are all purchased from Xinyang Leyan Biotechnology GmbH, and are dry powder products. The concentration of the pseudomonas dry powder is 50 hundred million cfu/g, the concentration of the acinetobacter dry powder is 80 hundred million cfu/g, and the concentration of the bacillus dry powder is 120 hundred million cfu/g.
The preparation method of the fermentation liquor of 3 strains comprises the following steps: inoculating 1g of the dry powder product into 1000ml of sterilized beef extract peptone liquid culture solution, and culturing for 24 hours at 28 ℃ under shaking (200rmp/s) in dark light. The beef extract peptone liquid culture medium comprises the following components in percentage by weight: 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl and 1000ml of water, and the pH value is 7.4-7.6.
Further, the processing method of the tea seed shells in the step (1) comprises the following steps: before use, the tea seed shells are exposed for 36-48h at the temperature of 33-37 ℃.
Further, in the step (1), 32.5-35 kg of tea seed shells are replaced by 30-40 kg of the ground pruned branches of the tea trees.
Further, the preparation method of the crushed pruned tea tree branches comprises the following steps: the lightly-trimmed and heavily-trimmed tea branches are firstly exposed for 60-72 hours at the temperature of 30-37 ℃, and mechanically crushed into particles of 1.2-1.5 cm multiplied by 2.1-3.0 cm.
Further, the mulberry variety in the step (2) is red fruit No. 2, 46CO19 or 8632.
Further, the nutrient solution obtained in the third step (7) comprises the following components in parts by weight: 0.02g of 2-chloroethyltrimethylammonium chloride, 0.15g of 4-iodophenoxyacetic acid, 0.12g of zeatin nucleoside, 0.06g of naphthylacetic acid, 0.05g of cycloheximide, 0.3g of dipotassium hydrogen phosphate, 0.12g of abscisic acid and 99g of PSP buffer solution. Wherein the PSP buffer solution comprises the following components in percentage by weight: 5g of glucose, 8g of magnesium sulfate, 0.5g of dipotassium phosphate, 2g of sodium chloride, 0.3g of ferrous sulfate heptahydrate, 2.0g of calcium carbonate, 1g of disodium ethylene diamine tetraacetate and 1000ml of water.
Further, the preparation method of the bactericide in the step (8) S1 is as follows: the bacillus subtilis and the brevibacillus brevis dry powder products are respectively diluted into 20 hundred million cfu/ml concentration by sterile water, and are respectively inoculated into fermentation culture solution according to the volume ratio of 5 percent, after shaking dark light culture for 48 hours at 28 ℃ and 180rmp/s, 3000-5000rmp/s centrifugation is carried out for 20-30min, and the supernatant is taken as a bactericide.
The bacillus subtilis and the brevibacillus brevis are purchased from Xinyang Leyao biotechnology limited company and are dry powder products. The concentration of the bacillus subtilis dry powder product is 200 hundred million cfu/g, and the concentration of the bacillus brevis dry powder product is 160 hundred million cfu/g. The fermentation culture solution comprises the following components in percentage by weight: 20-25g of bean cake powder, 10-15g of brown sugar, 1.5g of sodium chloride, 0.5g of calcium chloride, 0.5g of potassium sulfate, 0.3g of magnesium sulfate and 1000g of water.
Further, in the step (8), S5, the bait comprises 50-80g of Acer mono Maxim leaf crushed material, 20-30g of 50% mulberry juice, 30-50g of mature vinegar, 2-5g of 2.5% imidacloprid aqua and 100g of water.
According to the technical scheme, compared with the prior art, the fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries is systematically, scientifically and accurately butt against all risk points generated by pesticide residue, and the full coverage of a pesticide residue control link is realized from a soil source to pest control; (2) the mulberry obtained by the fruit mulberry green cultivation method provided by the invention has the quality reaching the A-level product standard of green food, wherein the pesticide residue of the sanitary standard is zero and exceeds the standard; (3) the mulberry obtained by the mulberry green cultivation method has the characteristics of good fruit shape, bright color, rich nutrition, safety and high quality.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Pseudomonas, Acinetobacter, Bacillus subtilis, and Brevibacillus brevis were all purchased from Xinyangli Leyao Biotech Co., Ltd and were dry powder products. The concentration of the pseudomonas dry powder is 50 hundred million cfu/g, the concentration of the acinetobacter dry powder is 80 hundred million cfu/g, the concentration of the bacillus dry powder is 120 hundred million cfu/g, the concentration of the bacillus subtilis dry powder product is 200 hundred million cfu/g, and the concentration of the brevibacillus brevis dry powder product is 160 hundred million cfu/g.
Example 1
A fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries comprises the following specific steps:
(1) preparing and cleaning planting soil:
selecting a loam plot with flat terrain, convenient irrigation and drainage, ventilation and sunny exposure and sterile nuclear disease, wherein the thickness of the soil layer is more than 50cm, and the pH value is about 5.2. And (4) applying 35kg and 32.5kg of microbial contamination reducing agent and crushed tea tree pruning branches into the planting field in 2016 (2 months and 6 days), and ploughing by using a root rotating machine to reach a ploughing depth of 60 cm.
The effective microorganism components of the microorganism pollution-reducing agent are as follows: pseudomonas bacteria: acinetobacter: a bacillus. Firstly, weighing 1g of dry powder products of three strains respectively, inoculating the dry powder products into 1000ml of sterilized beef extract peptone liquid culture solution, carrying out shake (200rmp/s) dark culture for 24 hours at 28 ℃ to obtain three-strain fermentation liquor, and then preparing the microbial contamination-reducing agent. The beef extract peptone liquid culture medium comprises the following components in percentage by weight: 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl and 1000ml of water, and the pH value is 7.4-7.6.
The microbial contamination-reducing agent comprises the following components in percentage by volume: 30% of pseudomonas fermentation liquor, 10% of acinetobacter fermentation liquor, 15% of bacillus fermentation liquor, 5% of uric acid, 800.5% of tween, 0.2% of boric acid, 0.5% of dipotassium phosphate, 0.5% of citric acid, 0.2% of copper sulfate, 0.3% of ferrous sulfate and 37.8% of water.
The processing mode of the crushed tea tree pruning branches is as follows: the lightly-trimmed and heavily-trimmed tea branches are firstly exposed for 60 hours at the temperature of 33-37 ℃, and mechanically crushed into particles of 1.2-1.5 cm multiplied by 2.1-3.0 cm.
(2) Selecting mulberry seedlings:
the mulberry variety is fructus crataegi No. 2, the mulberry seedling has uniform size, seedling diameter of 11.7-13.2mm, complete main root, and root length of more than 140 mm.
(3) Planting time: 2016 (2 months) and 26 days
(4) Planting density: 80 plants are planted per mu, the plant spacing is 2.2m, and the row spacing is 3.2 m.
(5) The planting method comprises the following steps:
before planting, trimming off the tender and weak part of the fruit mulberry seedling with the length of more than 60cm, and cutting the root system for 20 cm; hole planting is adopted, and the planting hole size is 50cm multiplied by 50 cm; before planting, applying 3.0kg of decomposed organic fertilizer to each planting hole, covering soil after applying the fertilizer and compacting to 10cm of the ground; spreading the root system of the seedling, placing the seedling in the center of the planting hole, strengthening the body, filling soil, slightly lifting, treading, and thoroughly watering the root fixing water.
(6) Tree-shaped cultivation:
after the planting, the part 40cm away from the ground is fixed to be dry. Thinning buds when the young shoots on the trunk grow to 10cm, selecting and reserving 2 robust young shoots which are uniformly distributed at the rooting positions, and culturing the young shoots into thick and strong branches. And in 2017, in 25 days in 2 months, cutting off branches at the position 70cm away from the ground of the fruit mulberry, and determining first-level branches. After sprouting, when young shoots grow to 10cm, thinning, selecting and reserving 3 young shoots on each branch, and pinching when the young shoots grow to 90cm away from the ground to form 6 secondary branches. And cutting a fixed fist at a position 90cm away from the ground of the mulberry fruit in the third year after fruit picking in 2018, namely 5 month and 17 days. After germination, 2 new shoots are reserved on each fist to form the fruit bearing branches. Later, the mulberry shoots are cut at the mulberry fist every year.
(7) Enhancement of tree vigor
Fertilizing: when mulberry buds sprout, 0.3kg of organic-inorganic compound fertilizer (N: P: K ═ 5:3:4) (organic matter content 30%, purchased from Henan Yubao Fertilizer Co., Ltd.) is applied to each plant; after harvesting and cutting mulberry, 0.4kg of organic-inorganic compound fertilizer (20% of organic matter, Happy soil fertilizer industry, Ltd.) and 2.0kg of organic fertilizer (40% of organic matter, Shandong Wanhao Co., Ltd.) are applied to each mulberry plant; after mulberry leaves fall, 4.0kg of organic fertilizer (45% organic matter, Shandong Wanhao Co., Ltd.) was applied to each mulberry. During fertilization, ditching and fertilizing near the water dripping line of the crown, and covering soil after fertilization.
And II, trimming: and (5) cutting annual fruit hanging branches at a mulberry fist after the mulberry harvesting work is completed in 5 months every year. When the buds on a mulberry fist germinate, thinning the buds with improper implantation positions, excessive density, weakness, over-strength and diseases and pests, controlling the number of branches of a single plant for bearing fruits to be 25, exposing the cut branches and leaves at a high temperature of more than 30 ℃ for more than 7 days, mechanically crushing the branches and leaves into particles with the size of 1.3-1.8cm multiplied by 2.1-2.5cm, returning the root rotating machine to the garden, and rotating the roots to the depth of 20-25 cm; after the mulberry leaves fall and before mulberry buds sprout from the bottom of 11 months to the beginning of 2 months in the next year, pruning dead branches, insect-plague branches, weak twigs, lateral lying branches, dead fists and the like, removing the prunes out of the garden, and adding lime water for centralized treatment.
Injecting nutrient solution: injecting 1000ml of tree vigor enhancing nutrient solution every 15 days from the beginning of flowering period to the olive period every year, wherein the nutrient solution comprises the following components: 0.02g of 2-chloroethyltrimethylammonium chloride, 0.15g of 4-iodophenoxyacetic acid, 0.12g of zeatin nucleoside, 0.06g of naphthylacetic acid, 0.05g of cycloheximide, 0.3g of dipotassium hydrogen phosphate, 0.12g of abscisic acid and 99g of PSP buffer solution. Wherein the PSP buffer solution comprises the following components in percentage by weight: 5g of glucose, 8g of magnesium sulfate, 0.5g of dipotassium phosphate, 2g of sodium chloride, 0.3g of ferrous sulfate heptahydrate, 2.0g of calcium carbonate, 1g of disodium ethylene diamine tetraacetate and 1000ml of water.
(8) Prevention and control of plant diseases and insect pests
S1, ground fabric use and cover: covering black single-side silty ground cloth on the mulberry orchard from 1 ten days to 2 middle days of each year according to the ratio of 185g/m2The standard is to perform full circle coverage. And at the end of 5 months, after the mulberry is harvested, collecting the ground fabric in time, cleaning, airing and storing for recycling, spraying 30% of bactericide for 7 days in advance before next use, sterilizing, and naturally airing for use.
The bacillus subtilis and the brevibacillus brevis dry powder products are respectively diluted into 20 hundred million cfu/ml concentration by sterile water, and are respectively inoculated into fermentation culture solution according to the volume ratio of 5 percent, after shaking dark light culture for 48 hours at 28 ℃ and 180rmp/s, 3000-5000rmp/s centrifugation is carried out for 20-30min, and the supernatant is taken as a bactericide.
The bacillus subtilis and the brevibacillus brevis are purchased from Xinyang Leyao biotechnology limited company and are dry powder products. The fermentation culture solution comprises the following components in percentage by weight: 20-25g of bean cake powder, 10-15g of brown sugar, 1.5g of sodium chloride, 0.5g of calcium chloride, 0.5g of potassium sulfate, 0.3g of magnesium sulfate and 1000g of water.
S2, controlling the mulberry psylla: and (3) from the end of 2 months to the beginning of the third month every year, namely when the mulberry sprouts, combining the field pest and disease prediction and prediction results to carry out green prevention and control on the mulberry psyllids. If the number of the ten-leaf insects in the field of the mulberry psyllids exceeds 4, the Papilea pteropii or trichogramma is released in time, and the ratio of the released bees to the insects per mu is 8: 1.
S3, preventing and treating sclerotinia rot: spraying 800 times of 50% procymidone or 900 times of 70% thiophanate-methyl solution in time from the beginning of flowering period to the young fruit period when the sclerotinia rot of the mulberry appears in the last ten days of 3 months to 4 months every year, alternately using the two solutions, spraying the two solutions once in 5 days, and stopping using the two solutions 25 days before harvesting.
S4, mulberry moth prevention and control: and (4) carrying out green prevention and control on the mulberry borers at the early 5-9 months of each year by combining field prediction results. If the population density of the mulberry borers in the field is below 5 per plant, suspending sex pheromone for trapping, and installing 2 in each mu; the population density of the mulberry borer is 5-15 per plant, 50ml of metarhizium anisopliae CQMa117 microgranule (purchased from Chongqing major biotechnology development Co., Ltd.) is sprayed per mu, and the dilution is 1000 times for prevention, wherein the effective bacteria concentration before the metarhizium anisopliae dilution is 10 hundred million/ml; and (3) pruning mulberry trees in summer when the population density of the mulberry borers exceeds 30 per tree, spraying metarhizium anisopliae to the pruned branches, and spraying 5kg of film to each ton of pruned branches and sealing for 72 hours.
S5, prevention and treatment of the mulberry longicorn: in the middle and late ten days of 6 months to 8 months every year, the longicorn is controlled by adopting a bait agent, the bait agent comprises 50g of the crushed material of the Acer nikoense leaves, 20g of 50% mulberry juice, 50g of mature vinegar, 5g of 2.5% imidacloprid aqua and 100g of water, and the bait agent is canned and hung at the position 0.7m above the ground and is replaced every 2 weeks.
S6, mulberry field weed treatment: adopts a grass-weed mode, starts drill seeding at the end of 3 months every year, and adopts the following grass seeds: the sowing amount per mu of the white clover, the oxtail grass and the ryegrass is as follows: 0.8 kg: 0.2 kg: 0.3kg, the sowing mode is mixed drill sowing, the drill sowing depth is 20cm, and the seeds are soaked in warm water at 40 ℃ for 24 hours before sowing.
Example 2
A fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries comprises the following specific steps:
(1) preparing and cleaning planting soil:
selecting a loam plot with flat terrain, convenient irrigation and drainage, ventilation and sunny exposure and sterile nuclear disease, wherein the thickness of the soil layer is more than 50cm, and the pH value is about 6.3. And (3) applying the microbial contamination reducing agent and the tea seed shells into the planting field in 2016, 11 months and 24 days, wherein the application amount of the microbial contamination reducing agent and the application amount of the tea seed shells are 38kg and 33kg respectively, and ploughing by adopting a root rotating machine, wherein the ploughing depth is 60 cm.
The effective microorganism components of the microorganism pollution-reducing agent are as follows: pseudomonas bacteria: acinetobacter: a bacillus. Firstly, weighing 1g of dry powder products of three strains respectively, inoculating the dry powder products into 1000ml of sterilized beef extract peptone liquid culture solution, carrying out shake (200rmp/s) dark culture for 24 hours at 28 ℃ to obtain three-strain fermentation liquor, and then preparing the microbial contamination-reducing agent. The beef extract peptone liquid culture medium comprises the following components in percentage by weight: 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl and 1000ml of water, and the pH value is 7.4-7.6.
The microbial contamination-reducing agent comprises the following components in percentage by volume: 32% of pseudomonas fermentation liquor, 11% of acinetobacter fermentation liquor, 16% of bacillus fermentation liquor, 4% of uric acid, 800.4% of tween, 0.3% of boric acid, 0.6% of dipotassium phosphate, 0.6% of citric acid, 0.25% of copper sulfate, 0.35% of ferrous sulfate and 34.5% of water.
The processing method of the tea seed shells comprises the following steps: before use, the tea seed shells are exposed for 36-48h at the temperature of 33-37 ℃.
(2) Selecting mulberry seedlings:
the variety of fruit mulberry is 46CO19, the fruit mulberry seedling has uniform size, seedling diameter of 12.4-16.7mm, complete main root and root length of more than 145 mm.
(3) Planting time: 2016 (12 months and 7 days)
(4) Planting density: 80 plants are planted per mu, the plant spacing is 2.2m, and the row spacing is 3.2 m.
(5) The planting method comprises the following steps:
before planting, trimming off the tender and weak part of the fruit mulberry seedling with the length of more than 60cm, and cutting the root system for 20 cm; hole planting is adopted, and the planting hole size is 50cm multiplied by 50 cm; before planting, applying 3.5kg of decomposed organic fertilizer to each planting hole, covering soil after applying the fertilizer and compacting to 5cm of the ground; spreading the root system of the seedling, placing the seedling in the center of the planting hole, strengthening the body, filling soil, slightly lifting, treading, and thoroughly watering the root fixing water.
(6) Tree-shaped cultivation:
after planting, fixing the mulberry leaves 45cm away from the ground. Thinning buds when the young shoots on the trunk grow to 12cm, selecting and reserving 2 robust young shoots which are uniformly distributed at the rooting positions, and culturing the young shoots into thick and strong branches. And 3, 2 days in 2017, cutting off branches at a position 75cm away from the ground of the fruit mulberry, and determining first-level branches. After sprouting, when young shoots grow to 12cm, thinning, selecting and reserving 3 young shoots on each branch, and pinching when the young shoots grow to 95cm away from the ground to form 6 secondary branches.
And cutting a fixed fist at a position 95cm away from the ground of the mulberry fruit in the third year after fruit picking in 2018, namely 5 month and 22 days. After germination, 3 new shoots are reserved on each fist to form the fruit bearing branches. Later, the mulberry shoots are cut at the mulberry fist every year.
(7) Enhancement of tree vigor
Fertilizing: when mulberry buds sprout, 0.5kg of organic-inorganic compound fertilizer (N: P: K: 5:3:4) (organic matter content 20%, purchased from Henan Yubao Fertilizer Co., Ltd.) is applied to each plant; after harvesting and cutting mulberry, 0.2kg of organic-inorganic compound fertilizer (35% of organic matter, Happy land fertilizer industry, Inc. in Anyang city) and 2.0kg of organic fertilizer (40% of organic matter, Wanhao, Shandong, Co., Ltd.) are applied to each mulberry plant; after mulberry leaves fall, 5.0kg of organic fertilizer (35% of organic matter, Shandong Wanhao Co., Ltd.) was applied to each mulberry. During fertilization, ditching and fertilizing near the water dripping line of the crown, and covering soil after fertilization.
And II, trimming: and (5) cutting annual fruit hanging branches at a mulberry fist after the mulberry harvesting work is completed in 5 months every year. When the buds on a mulberry fist germinate, thinning the buds with improper implantation positions, excessive density, weakness, over-strength and diseases and pests, controlling the number of branches of a single plant for bearing fruits to be 25, exposing the cut branches and leaves at a high temperature of more than 30 ℃ for more than 7 days, mechanically crushing the branches and leaves into particles with the size of 1.3-1.8cm multiplied by 2.1-2.5cm, returning the root rotating machine to the garden, and rotating the roots to the depth of 20-25 cm; after the mulberry leaves fall and before mulberry buds sprout from the bottom of 11 months to the beginning of 2 months in the next year, pruning dead branches, insect-plague branches, weak twigs, lateral lying branches, dead fists and the like, removing the prunes out of the garden, and adding lime water for centralized treatment.
Injecting nutrient solution: injecting 1000ml of tree vigor enhancing nutrient solution every 15 days from the beginning of flowering period to the olive period every year, wherein the nutrient solution comprises the following components: 0.02g of 2-chloroethyltrimethylammonium chloride, 0.15g of 4-iodophenoxyacetic acid, 0.12g of zeatin nucleoside, 0.06g of naphthylacetic acid, 0.05g of cycloheximide, 0.3g of dipotassium hydrogen phosphate, 0.12g of abscisic acid and 99g of PSP buffer solution.
(8) Prevention and control of plant diseases and insect pests
S1, ground fabric use and cover: covering black single-side silty ground cloth on the mulberry orchard from 1 ten days to 2 middle days of each year according to the ratio of 185g/m2The standard is to perform full circle coverage. And at the end of 5 months, after the mulberry is harvested, collecting the ground fabric in time, cleaning, airing and storing for recycling, spraying 30% of bactericide for 7 days in advance before next use, sterilizing, and naturally airing for use.
The bacillus subtilis and the brevibacillus brevis dry powder products are respectively diluted into 20 hundred million cfu/ml concentration by sterile water, and are respectively inoculated into fermentation culture solution according to the volume ratio of 5 percent, after shaking dark light culture for 48 hours at 28 ℃ and 180rmp/s, 3000-5000rmp/s centrifugation is carried out for 20-30min, and the supernatant is taken as a bactericide.
The bacillus subtilis and the brevibacillus brevis are purchased from Xinyang Leyao biotechnology limited company and are dry powder products. The fermentation culture solution comprises the following components in percentage by weight: 20-25g of bean cake powder, 10-15g of brown sugar, 1.5g of sodium chloride, 0.5g of calcium chloride, 0.5g of potassium sulfate, 0.3g of magnesium sulfate and 1000g of water.
S2, controlling the mulberry psylla: and (3) from the end of 2 months to the beginning of the third month every year, namely when the mulberry sprouts, combining the field pest and disease prediction and prediction results to carry out green prevention and control on the mulberry psyllids. If the number of the ten-leaf insects in the field of the mulberry psyllids exceeds 4, the Papilea pteropii or trichogramma is released in time, and the ratio of the released bees to the insects per mu is 8: 1.
S3, preventing and treating sclerotinia rot: spraying 1000 times of 50% procymidone or 800 times of 70% thiophanate-methyl solution in time from the beginning of flowering period to the young fruit period when the sclerotinia rot of the mulberry appears in the last ten days of 3 months to 4 months every year, alternately using the two solutions, spraying once in 6 days, and stopping using the two solutions 25 days before harvesting.
S4, mulberry moth prevention and control: and (4) carrying out green prevention and control on the mulberry borers at the early 5-9 months of each year by combining field prediction results. If the population density of the mulberry borers in the field is below 5 per plant, suspending sex pheromone for trapping, and installing 2 in each mu; the population density of the mulberry borers is 5-15 per plant, 50ml of metarhizium anisopliae is sprayed per mu, the metarhizium anisopliae is diluted by 1000 times for prevention and treatment, and the concentration of the metarhizium anisopliae before dilution is 10 hundred million/ml; and (3) pruning mulberry trees in summer when the population density of the mulberry borers exceeds 30 per tree, spraying metarhizium anisopliae to the pruned branches, and spraying 5kg of film to each ton of pruned branches and sealing for 72 hours.
S5, prevention and treatment of the mulberry longicorn: in the middle and late ten days of 6 months to 8 months every year, the longicorn is controlled by adopting a bait agent, the bait agent comprises 60g of the crushed material of the Acer nikoense leaves, 20g of 50% mulberry juice, 30g of mature vinegar, 3g of 2.5% imidacloprid aqua and 100g of water, and the bait agent is canned and hung at the position 0.7m above the ground and is replaced every 2 weeks.
S6, mulberry field weed treatment: adopts a grass-weed mode, starts drill seeding at the end of 3 months every year, and adopts the following grass seeds: the sowing amount per mu of the white clover, the oxtail grass and the ryegrass is as follows: 0.6 kg: 0.4 kg: 0.5kg, soaking the seeds in warm water at 40 ℃ for 24 hours before sowing, and the drilling depth is 20 cm.
Example 3
A fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries comprises the following specific steps:
(1) preparing and cleaning planting soil:
selecting a loam plot with flat terrain, convenient irrigation and drainage, ventilation and sunny exposure and sterile nuclear disease, wherein the thickness of the soil layer is more than 50cm, and the pH value is about 7.0. And (4) applying the microbial contamination reducing agent and the tea seed shells into the planting field in 2016 (2 months) and 11 days, wherein the application amount of the microbial contamination reducing agent and the application amount of the tea seed shells are 40kg and 35kg respectively, and ploughing by adopting a root rotating machine, wherein the ploughing depth is 80 cm.
The effective microorganism components of the microorganism pollution-reducing agent are as follows: pseudomonas bacteria: acinetobacter: a bacillus. Firstly, weighing 1g of dry powder products of three strains respectively, inoculating the dry powder products into 1000ml of sterilized beef extract peptone liquid culture solution, carrying out shake (200rmp/s) dark culture for 24 hours at 28 ℃ to obtain three-strain fermentation liquor, and then preparing the microbial contamination-reducing agent. The beef extract peptone liquid culture medium comprises the following components in percentage by weight: 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl and 1000ml of water, and the pH value is 7.4-7.6.
The microbial contamination-reducing agent comprises the following components in percentage by volume: 35% of pseudomonas fermentation liquor, 12% of acinetobacter fermentation liquor, 18% of bacillus fermentation liquor, 3% of uric acid, 800.3% of tween, 0.3% of boric acid, 0.8% of dipotassium phosphate, 0.6% of citric acid, 0.25% of copper sulfate, 0.35% of ferrous sulfate and 29.4% of water.
The preparation method of the tea seed shells comprises the following steps: exposing the tea seed shells at 33-37 ℃ for 36h before use;
(2) selecting mulberry seedlings:
the mulberry variety is 8632 hybrid, and the mulberry seedlings are uniform in size, 12.2-17.6mm in seedling diameter, complete in main root and more than 133mm in root length.
(3) Planting time: 27/2/2017
(4) Planting density: 100 plants are planted per mu, the plant spacing is 2.2m, and the row spacing is 3.0 m.
(5) The planting method comprises the following steps:
before planting, trimming off tender and weak parts of the fruit mulberry seedlings, which are more than 70cm, and cutting root systems for 20 cm; hole planting is adopted, and the planting hole size is 50cm multiplied by 50 cm; applying 5.0kg of decomposed organic fertilizer to each planting hole before planting, covering soil after applying the fertilizer and compacting to 5cm above the ground; spreading the root system of the seedling, placing the seedling in the center of the planting hole, strengthening the body, filling soil, slightly lifting, treading, and thoroughly watering the root fixing water.
(6) Tree-shaped cultivation:
after planting, fixing the mulberry at a position 50cm away from the ground. And thinning the buds when the young shoots on the trunk grow to 15cm, selecting and reserving 3 robust young shoots which are uniformly distributed at the rooting positions, and culturing the robust young shoots into thick and strong branches. And 3, in 2017, in 25 days in 3 months, cutting off branches at a position 80cm away from the ground, and determining primary branches. After sprouting, when young shoots grow to 15cm, thinning, selecting and reserving 2 young shoots on each branch, and pinching when the young shoots grow to 100cm away from the ground to form 6 secondary branches. And cutting a fixed fist at a position 100cm away from the ground in the mulberry field after the fruits are harvested in the third year, namely 5 months and 27 days in 2018. After germination, 3 new shoots are reserved on each fist to form the fruit bearing branches. Later, the mulberry shoots are cut at the mulberry fist every year.
(7) Enhancement of tree vigor
Fertilizing: when mulberry buds sprout, 0.5kg of organic-inorganic compound fertilizer (N: P: K: 5:3:4) (organic matter content 15%, purchased from Henan Yubao Fertilizer Co., Ltd.) is applied to each plant; after harvesting and cutting mulberry, 0.5kg of organic-inorganic compound fertilizer (15% of organic matter, Happy soil fertilizer industry, Inc. in Anyang city) and 2.0kg of organic fertilizer (45% of organic matter, Wanhao, Shandong, Co., Ltd.) are applied to each mulberry plant; after mulberry leaves fall, organic fertilizer is applied again, and 5.0kg of organic matter (40% of organic matter, Shandong Wanhao Co., Ltd.) is applied to each mulberry. During fertilization, ditching and fertilizing near the water dripping line of the crown, and covering soil after fertilization.
And II, trimming: and (5) cutting annual fruit hanging branches at a mulberry fist after the mulberry harvesting work is completed in 5 months every year. When the buds on a mulberry fist germinate, thinning the buds with improper implantation positions, excessive density, weakness, over-strength and diseases and pests, controlling the number of branches of a single plant for bearing fruits to be 25, exposing the cut branches and leaves at a high temperature of more than 30 ℃ for more than 7 days, mechanically crushing the branches and leaves into particles with the size of 1.3-1.8cm multiplied by 2.1-2.5cm, returning the root rotating machine to the garden, and rotating the roots to the depth of 20-25 cm; after the mulberry leaves fall and before mulberry buds sprout from the bottom of 11 months to the beginning of 2 months in the next year, pruning dead branches, insect-plague branches, weak twigs, lateral lying branches, dead fists and the like, removing the prunes out of the garden, and adding lime water for centralized treatment.
Injecting nutrient solution: injecting 1000ml of tree vigor enhancing nutrient solution every 15 days from the beginning of flowering period to the olive period every year, wherein the nutrient solution comprises the following components: 0.02g of 2-chloroethyltrimethylammonium chloride, 0.15g of 4-iodophenoxyacetic acid, 0.12g of zeatin nucleoside, 0.06g of naphthylacetic acid, 0.05g of cycloheximide, 0.3g of dipotassium hydrogen phosphate, 0.12g of abscisic acid and 99g of PSP buffer solution.
(8) Prevention and control of plant diseases and insect pests
S1, ground fabric use and cover: covering black single-side silty ground cloth on the mulberry orchard from 1 ten days to 2 middle days of each year according to the ratio of 185g/m2The standard is to perform full circle coverage. And at the end of 5 months, after the mulberry is harvested, collecting the ground fabric in time, cleaning, airing and storing for recycling, spraying 30% of bactericide for 7 days in advance before next use, sterilizing, and naturally airing for use.
The bacillus subtilis and the brevibacillus brevis dry powder products are respectively diluted into 20 hundred million cfu/ml concentration by sterile water, and are respectively inoculated into fermentation culture solution according to the volume ratio of 5 percent, after shaking dark light culture for 48 hours at 28 ℃ and 180rmp/s, 3000-5000rmp/s centrifugation is carried out for 20-30min, and the supernatant is taken as a bactericide.
The bacillus subtilis and the brevibacillus brevis are purchased from Xinyang Leyao biotechnology limited company and are dry powder products. The fermentation culture solution comprises the following components in percentage by weight: 20-25g of bean cake powder, 10-15g of brown sugar, 1.5g of sodium chloride, 0.5g of calcium chloride, 0.5g of potassium sulfate, 0.3g of magnesium sulfate and 1000g of water.
S2, controlling the mulberry psylla: and (3) from the end of 2 months to the beginning of the third month every year, namely when the mulberry sprouts, combining the field pest and disease prediction and prediction results to carry out green prevention and control on the mulberry psyllids. If the number of the ten-leaf insects in the field of the mulberry psyllids exceeds 4, the Papilea pteropii or trichogramma is released in time, and the ratio of the released bees to the insects per mu is 8: 1.
S3, preventing and treating sclerotinia rot: spraying 1000 times of 50% procymidone or 800 times of 70% thiophanate-methyl solution in time from the beginning of flowering period to the young fruit period when the sclerotinia rot of the mulberry appears in the last ten days of 3 months to 4 months every year, alternately using the two solutions, spraying the two solutions once in 5 days, and stopping using the two solutions 25 days before harvesting.
S4, mulberry moth prevention and control: and (4) carrying out green prevention and control on the mulberry borers at the early 5-9 months of each year by combining field prediction results. If the population density of the mulberry borers in the field is below 5 per plant, suspending sex pheromone for trapping, and installing 2 in each mu; the population density of the mulberry borer is 5-15 per plant, 60ml of metarhizium anisopliae CQMa117 microgranules (purchased from Chongqing major biotechnology development Co., Ltd.) are sprayed per mu, the dilution is 1100 times for prevention and treatment, and the effective bacteria concentration before the metarhizium anisopliae dilution is 10 hundred million/ml; and (3) pruning mulberry trees in summer when the population density of the mulberry borers exceeds 30 per tree, spraying metarhizium anisopliae to the pruned branches, and spraying 5kg of film to each ton of pruned branches and sealing for 72 hours.
S5, prevention and treatment of the mulberry longicorn: in the middle and late ten days of 6 months to 8 months every year, the longicorn is controlled by adopting a bait agent, the bait agent comprises 60g of the crushed material of the Acer nikoense leaves, 20g of 50% mulberry juice, 30g of mature vinegar, 3g of 2.5% imidacloprid aqua and 100g of water, and the bait agent is canned and hung at the position 0.7m above the ground and is replaced every 2 weeks.
S6, mulberry field weed treatment: adopts a grass-weed mode, starts drill seeding at the end of 3 months every year, and adopts the following grass seeds: the sowing amount per mu of the white clover, the oxtail grass and the ryegrass is as follows: 0.5 kg: 0.4 kg: 0.3kg, soaking the seeds in warm water at 40 ℃ for 24 hours before sowing, and the drilling depth is 20 cm.
The mature mulberries obtained by the cultivation technology of the embodiment 1-3 of the invention have good fruit types, bright color, purple, dark purple and black main materials, sufficient water, and no weak fruits, residual fruits and diseased fruits.
Mature mulberries are picked for carrying out 24 hygiene index detections on soluble solid matters, procymidone, thiophanate-methyl and the like. The results are shown in Table 1.
TABLE 1
Figure BDA0002455937700000151
Figure BDA0002455937700000161
Note: -indicating that the detection value is not detected; denotes that the reference value is not labeled.
The results in Table 1 show that 19.4%, 16.4% and 13.6% of the soluble solids in examples 1, 2 and 3 all reach the standard (11.05% or more) of the soluble solids in NT/T844-2010 of green food temperate fruit; in 24 hygiene indexes, except for procymidone and thiophanate-methyl, other 22 hygiene indexes (inorganic arsenic, lead, cadmium, total mercury, fluorine, chromium, hexachloro, dichlorovos, dichlorvos, parathion, malathion, phorate, fenitrothion, fenthion, deltamethrin, dipterex, chlorothalonil, carbendazim and triadimefon) are not detected, and 22 hygiene index detection results reach and obviously exceed the requirements of green food temperate fruit NY/T844-2010.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (7)

1. A fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries is characterized by comprising the following specific steps:
(1) preparing and cleaning planting soil:
selecting a loam plot with flat terrain, convenient irrigation and drainage, ventilation and sunny exposure and sterile nuclear disease, wherein the thickness of a soil layer is more than 50cm, and the pH value is 5.0-7.5; applying 35-40 kg of microbial contamination reducing agent and 32.5-35 kg of tea seed shells to each mu of planting land 15-20 days before planting, and ploughing to a depth of 60-80 cm;
(2) selecting mulberry seedlings:
selecting excellent hybrid mulberry varieties with strong disease resistance, waterlogging tolerance, close planting and low dryness, wherein mulberry seedlings are uniform in size, the diameter of the seedlings is 10.0-15.0mm, and the main roots are complete;
(3) planting time:
in the middle and last ten days of 2 months or from the bottom of 11 months to the beginning of 12 months every year;
(4) planting density:
planting 70-100 plants per mu, wherein the plant spacing is 2.0-2.5 m, and the row spacing is 3.0-4.0 m;
(5) the planting method comprises the following steps:
before planting, trimming off the tender and weak part of the fruit mulberry seedling with the length of 60-70 cm, and cutting the root system for 20 cm; hole planting is adopted, and the planting hole size is 50cm multiplied by 50 cm; before planting, applying 3.0 kg-5.0 kg of decomposed organic fertilizer in each planting hole, covering soil after applying the fertilizer and treading the soil to be 5cm-10cm lower than the ground; spreading the root system of the seedling, placing the seedling in the center of a planting hole, strengthening the body, filling soil, slightly lifting, treading, and thoroughly watering the root fixing water;
(6) tree-shaped cultivation:
after field planting, drying the mulberry leaves 40-50 cm away from the ground; thinning buds when the young shoots on the trunk grow to 10-15 cm, selecting and reserving 2-3 healthy young shoots which are uniformly distributed at the rooting positions, and culturing the young shoots into thick and strong branches;
before the end of 3 months next year, primary branch stems are fixed at the positions of fruit mulberry which are 70 cm-80 cm away from the ground; after sprouting, when young shoots grow to 10-15 cm, thinning the sprouts, selecting and reserving 2-3 young shoots on each primary branch, and pinching when the young shoots grow to 90-100 cm away from the ground to form 3-6 secondary branches;
cutting a fixed fist at the position of 90-100 cm away from the ground after picking fruits in the third year; after sprouting, keeping 2-4 young shoots on each fist, and cultivating into fruit bearing branches; cutting at mulberry fist every year;
(7) enhancement of tree vigor
Fertilizing:
when mulberry buds sprout, 0.2 kg-0.5 kg of organic-inorganic compound fertilizer N: P: K =5:3:4 is applied to each plant; after harvesting and cutting mulberry, applying 0.2 kg-0.5 kg of organic-inorganic compound fertilizer N: P: K =4:3:1 and 2.0 kg-3.0 kg of organic fertilizer to each mulberry; after mulberry leaves fall, applying organic fertilizer again, wherein 4.0 kg-5.0 kg of organic fertilizer is applied to each mulberry; during fertilization, ditching and fertilizing near the water dripping line of the crown, and covering soil after fertilization;
and II, trimming:
cutting annual fruit hanging branches at a mulberry fist after the mulberry harvesting work is completed in 5 months every year; when the buds on a mulberry fist germinate, thinning the buds with improper implantation positions, excessive density, weakness, over-strength and diseases and pests, controlling the number of branches for bearing fruits of a single plant to be 25-30, exposing the cut branches and leaves at a high temperature of more than 30 ℃ for more than 7 days, mechanically crushing the branches and leaves into particles with the size of 1.3-1.8cm multiplied by 2.1-2.5cm, returning the rotary cultivator to the garden, and carrying out rotary tillage with the depth of 20-25 cm; after the mulberry leaves fall and before mulberry buds sprout at the end of 11 months every year to the beginning of 2 months next year, pruning dead branches, insect-plague branches, weak twigs, lateral lying branches and dead fists, removing the prunes out of the garden, and adding lime water for centralized treatment;
injecting nutrient solution:
injecting 1000ml of tree vigor enhancing nutrient solution every 15 days from the beginning of flowering to the olive period every year;
(8) prevention and control of plant diseases and insect pests
S1, ground fabric use and cover: covering black single-side silty ground cloth on the mulberry orchard from 1 ten days to 2 middle days of each year according to the ratio of 185g/m2Performing full-circle coverage according to the standard; at the end of 5 months, wait for mulberryAfter harvesting, collecting the ground fabric in time, spraying 30% of bactericide for 7 days in advance before next use, sterilizing, and naturally drying for use;
s2, controlling the mulberry psylla: carrying out mulberry field mulberry psylla prediction and forecast when mulberry sprouts from the end of 2 months to the beginning of the third month every year, releasing Paederus formosanus or trichogramma when the number of ten-leaf worms in mulberry field exceeds 4, and releasing according to the bee-worm release ratio of 8:1 per mu;
s3, preventing and treating sclerotinia rot: spraying liquid which is 800-1000 times of 50% procymidone or liquid which is 800-1000 times of 70% thiophanate-methyl in time when sclerotinia rot of mulberry appears from the beginning of flowering period to the young fruit period in each year from late 3-4 months to the middle-upper ten days of 4 months, alternately using the two, spraying the two once in 5-6 days, and stopping using the two 25 days before harvesting;
s4, mulberry moth prevention and control: at the beginning of 5 months to 9 months every year, carrying out the field prediction of the mulberry borers; when the population density of the mulberry borers in the field is below 5 per plant, suspending sex pheromone for trapping, and installing 2 in each mu; when the population density of the mulberry borers is 5-15 per plant, 50-60 ml of metarhizium anisopliae is sprayed per mu, the metarhizium anisopliae is diluted by 1200 times for prevention and treatment, and the concentration of the metarhizium anisopliae before dilution is 10-12 hundred million spores/ml; pruning mulberry trees in summer when the population density of the mulberry borers exceeds 30 per plant;
s5, prevention and treatment of the mulberry longicorn: in the middle and late 6 months to 8 months, the longicorn is prevented and controlled by adopting a bait agent, the bait agent is suspended at a position 0.7m above the ground in a canning manner, and is replaced every 2 weeks;
s6, mulberry field weed treatment: adopts a mode of treating grass by grass, and the grass seeds are as follows: the sowing amount per mu of the white clover, the oxtail grass and the ryegrass is as follows: 0.5-0.8 kg: 0.2-0.5 kg: 0.3-0.5kg, the sowing mode is mixed drill sowing, the drill sowing depth is 20cm, and the sowing time is 3 months per year;
the microorganism pollution reducing agent in the step (1) comprises the following specific components: 30-35% of pseudomonas fermentation liquor, 10-12% of acinetobacter fermentation liquor, 15-18% of bacillus fermentation liquor, 3-5% of uric acid, 0-0.5% of tween 800.3, 0.2-0.3% of boric acid, 0.5-0.8% of dipotassium phosphate, 0.5-0.6% of citric acid, 0.2-0.25% of copper sulfate, 0.3-0.35% of ferrous sulfate and 29-40% of water;
the third step (7) is that the nutrient solution comprises the following components by weight: 0.02g of 2-chloroethyltrimethylammonium chloride, 0.15g of 4-iodophenoxyacetic acid, 0.12g of zeatin nucleoside, 0.06g of naphthylacetic acid, 0.05g of cycloheximide, 0.3g of dipotassium hydrogen phosphate, 0.12g of abscisic acid and 99g of PSP buffer solution.
2. The fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries according to claim 1, wherein the tea seed hulls treatment method in the step (1) comprises the following steps: before use, the tea seed shells are exposed for 36-48h at the temperature of 33-37 ℃.
3. The green mulberry cultivation method capable of effectively reducing pesticide residue of mulberries according to claim 1, wherein in the step (1), 32.5-35 kg of tea seed shells are replaced by 30-40 kg of crushed pruned tea tree branches.
4. The green mulberry cultivation method capable of effectively reducing pesticide residue of mulberries according to claim 3, wherein the preparation method of the pruned branch crushed material of tea trees comprises the following steps: the lightly-trimmed and heavily-trimmed tea branches are firstly exposed for 60-72 hours at the temperature of 30-37 ℃, and mechanically crushed into particles of 1.2-1.5 cm multiplied by 2.1-3.0 cm.
5. The mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries according to claim 1, wherein the mulberry varieties in the step (2) are red fruit No. 2, 46CO19 or 8632.
6. The mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries according to claim 1, wherein the preparation method of the bactericide in step (8) S1 comprises the following steps: the bacillus subtilis and the brevibacillus brevis dry powder products are respectively diluted into 20 hundred million cfu/ml concentration by sterile water, and are respectively inoculated into fermentation culture solution according to the volume ratio of 5 percent, after shaking and dark light culture for 48 hours at 28 ℃, and 180rpm, the products are centrifuged for 20-30min at 3000 rpm and 5000rpm, and the supernatant is taken as a bactericide.
7. The mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries according to claim 1, wherein the bait agent in step (8) S5 comprises 50-80g of Acer mono Maxim leaf crushed material, 20-30g of 50% mulberry juice, 30-50g of mature vinegar, 2-5g of 2.5% imidacloprid aqua and 100g of water.
CN202010306434.XA 2020-04-17 2020-04-17 Fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries Expired - Fee Related CN111492877B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010306434.XA CN111492877B (en) 2020-04-17 2020-04-17 Fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010306434.XA CN111492877B (en) 2020-04-17 2020-04-17 Fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries

Publications (2)

Publication Number Publication Date
CN111492877A CN111492877A (en) 2020-08-07
CN111492877B true CN111492877B (en) 2021-10-29

Family

ID=71848313

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010306434.XA Expired - Fee Related CN111492877B (en) 2020-04-17 2020-04-17 Fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries

Country Status (1)

Country Link
CN (1) CN111492877B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115226556A (en) * 2022-08-05 2022-10-25 江西省经济作物研究所 Ecological mulberry planting method capable of simultaneously reducing sclerotinia rot and bird damage of mulberry
CN115474502A (en) * 2022-10-09 2022-12-16 四川省阆中蚕种场 Planting method for low-stem, close planting and rapid breeding of mulberry trees

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103865843B (en) * 2014-02-13 2016-03-09 河北省科学院生物研究所 A kind of microbial composite bacteria and the application in control vegetable fungi disease thereof
CN104782409A (en) * 2015-04-01 2015-07-22 溧阳市天目湖农业发展有限公司 Fruit and leaf mulberry variety breeding and efficient cultivation mode
CN105028111B (en) * 2015-09-15 2018-05-04 管天球 Method is planted between a kind of oil tea and mulberry leaf
CN105384534B (en) * 2015-12-15 2019-04-12 山水环境科技股份有限公司 A kind of liquid organic biofertilizer and preparation method thereof
CN106045714A (en) * 2016-07-11 2016-10-26 浏阳市聚康茶油专业合作社 Organic fertilizer produced from tea seed meal and tea seed shells and preparing method thereof
CN106212119A (en) * 2016-07-12 2016-12-14 江西省蚕桑茶叶研究所 A kind of method of effective prevention and control diseases of mulberry fruits
CN107619788A (en) * 2016-07-21 2018-01-23 济南航晨生物科技有限公司 A kind of agricultural bacillus bacterium solution long-effective protection agent prescription and preparation method thereof
CN106747724A (en) * 2016-11-30 2017-05-31 广西慧投互联网金融服务有限公司 A kind of organic biofertilizer and preparation method thereof
CN108184529B (en) * 2018-01-25 2019-12-03 江西省蚕桑茶叶研究所 A kind of cultural method improving dish mulberry tree burgeen yield and quality
CN108967091A (en) * 2018-07-10 2018-12-11 关岭玉盛堂农业药业有限公司 A kind of implantation methods of rhizoma polygonati
CN109121991A (en) * 2018-09-29 2019-01-04 阜阳市辉源果桑科技产业园有限公司 A kind of mulberries implantation methods
CN110357729A (en) * 2019-08-23 2019-10-22 山东捷利尔肥业有限公司 A kind of photosynthetic biological agent of enhancing pomegranate

Also Published As

Publication number Publication date
CN111492877A (en) 2020-08-07

Similar Documents

Publication Publication Date Title
CN104012270A (en) Planting method of pollution-free balsam pear
CN107466657B (en) Peanut planting method
CN107439217B (en) Pollution-free cultivation method for fruit type sweet potatoes
CN101366337A (en) Cultivation and grow method of high-quality asparagus
CN107624489A (en) A kind of implantation methods of selenium-rich lichee
CN111492877B (en) Fruit mulberry green cultivation method capable of effectively reducing pesticide residue of mulberries
Ashilenje Learn how to grow Peppers
CN109328921B (en) Sweet corn planting method capable of achieving three harvests per year
CN104272968A (en) Hunan polygonatum odoratum planting and production method
Dwiastuti Citrus foot rot disease (Phytophthora spp.) control in Indonesia using good agricultural practices efforts green agroindustry
CN110547166A (en) method for root-limiting cultivation of polygonatum cyrtonema under high-gradient forest
CN110073916A (en) A kind of Snakegourd Fruit implantation methods
CN114096148B (en) Method for simultaneously controlling weeds and planting target plants by using plant materials
CN113016513B (en) Sesame and saffron intercropping and interplanting method
CN106472591B (en) Ecological insecticidal bactericide and preparation method thereof
CN113207536A (en) Woody honeysuckle cultivation method
CN110972790A (en) Comprehensive organic cultivation method for non-vine fruit trees
CN109197353A (en) A kind of implantation methods of the crisp persimmon of sweet tea
CN109429934A (en) A kind of Siraitia grosvenorii and early-maturing Maodou interplanting method
CN110521511B (en) Pitaya brown rot comprehensive prevention and control method based on precise pesticide application and application thereof
Medina-Urrutia et al. Organic mango production: a review Campo Experimental Tecomán, Mexico
CN105900661A (en) Organic bean planting technology
CN106508358B (en) Method for reducing diseases and improving yield in water chestnut cultivation
Sarfaraz et al. Seed Production of Chilli (Capsicum frutescense)
Pandey et al. Organic cultivation practices of Sikkim mandarin

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20211029

CF01 Termination of patent right due to non-payment of annual fee