CN1114613C - 蛋白质溶液的铝/铁处理和膜浓缩方法 - Google Patents

蛋白质溶液的铝/铁处理和膜浓缩方法 Download PDF

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Publication number
CN1114613C
CN1114613C CN96194380.7A CN96194380A CN1114613C CN 1114613 C CN1114613 C CN 1114613C CN 96194380 A CN96194380 A CN 96194380A CN 1114613 C CN1114613 C CN 1114613C
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China
Prior art keywords
protein solution
test
aluminum
concentration
protein
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Expired - Fee Related
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CN96194380.7A
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English (en)
Chinese (zh)
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CN1186498A (zh
Inventor
T·K·尼尔森
M·A·拉斯特森
N－V·尼尔森
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Novozymes AS
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Novozymes AS
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN96194380.7A 1995-06-02 1996-05-31 蛋白质溶液的铝/铁处理和膜浓缩方法 Expired - Fee Related CN1114613C (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK0626/95 1995-06-02
DK62695 1995-06-02
DK0626/1995 1995-06-02

Publications (2)

Publication Number Publication Date
CN1186498A CN1186498A (zh) 1998-07-01
CN1114613C true CN1114613C (zh) 2003-07-16

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ID=8095720

Family Applications (1)

Application Number Title Priority Date Filing Date
CN96194380.7A Expired - Fee Related CN1114613C (zh) 1995-06-02 1996-05-31 蛋白质溶液的铝/铁处理和膜浓缩方法

Country Status (9)

Country Link
EP (1) EP0828753B1 (enExample)
JP (1) JPH11511118A (enExample)
CN (1) CN1114613C (enExample)
AT (1) ATE235506T1 (enExample)
AU (1) AU5996996A (enExample)
DE (1) DE69626976T2 (enExample)
DK (1) DK0828753T3 (enExample)
IN (1) IN181859B (enExample)
WO (1) WO1996038469A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2810667B1 (fr) * 2000-06-23 2004-09-03 Warner Lambert Co Procede d'isolement et de purification d'une proteine, et proteine obtenue
US7659102B2 (en) 2001-02-21 2010-02-09 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
ATE510023T1 (de) 2001-02-21 2011-06-15 Verenium Corp Enzyme mit alpha-amylase-aktivität und verfahren zu deren verwendung
US20030013172A1 (en) * 2001-05-14 2003-01-16 Joel Gerendash Novel methods of enzyme purification
EP1412384B1 (en) 2001-06-28 2007-12-26 Novo Nordisk A/S Stable formulation of modified glp-1
CN100487114C (zh) * 2001-12-11 2009-05-13 诺维信公司 从发酵液收获晶体
US7118891B2 (en) 2001-12-11 2006-10-10 Novozymes A/S Crystal harvest from fermentation broth
AU2004229313B2 (en) 2003-03-06 2010-08-19 Basf Enzymes Llc Amylases, nucleic acids encoding them and methods for making and using them
EP1633391B1 (en) 2003-06-03 2011-10-19 Novo Nordisk A/S Stabilized pharmaceutical peptide compositions
BRPI0410972C1 (pt) 2003-06-03 2021-05-25 Novo Nordisk As método para aumentar a vida de armazenagem de uma composição farmacêutica, composição farmacêutica, e, método para tratamento de hiperglicemia
DK3300721T4 (da) 2003-11-20 2025-03-03 Novo Nordisk As Propylenglycol-holdige peptidformuleringer hvilke er optimale til fremstilling og til anvendelse i injektionsindretninger
PL1789434T3 (pl) 2004-08-31 2014-07-31 Novo Nordisk As Zastosowanie tris(hydroksymetylo)aminometanu do stabilizacji peptydów, polipeptydów i białek
CN106137952B (zh) 2004-11-12 2020-11-17 诺和诺德公司 促胰岛素肽的稳定制剂
CA2620193C (en) * 2005-09-15 2016-02-16 Wyeth Separation of protein from impurities using salts
MX2009006782A (es) 2006-12-21 2009-09-21 Verenium Corp Amilasas y glucoamilasas, acidos nucleicos que las codifican y metodos para formarlas y usarlas.
US20120252066A1 (en) * 2011-03-29 2012-10-04 Heng Meng H Methods of foam control
US20150291656A1 (en) 2012-11-01 2015-10-15 Novozymes A/S Method for removal of dna
CN105745217A (zh) 2013-11-19 2016-07-06 凯米罗总公司 减少生物技术液体的颜色的方法
TWI847306B (zh) 2017-08-24 2024-07-01 丹麥商諾佛 儂迪克股份有限公司 Ｇｌｐ-１組成物及其用途
CN108949703A (zh) * 2018-06-28 2018-12-07 大连理工大学 一种半纤维小体类似物粗提纯方法
US12146171B2 (en) 2019-06-13 2024-11-19 Basf Se Method of recovering a protein from fermentation broth using a divalent cation
BR112022013795A2 (pt) 2020-02-18 2022-09-13 Novo Nordisk As Composição farmacêutica líquida, e, kit
BR112023005111A2 (pt) 2020-09-22 2023-04-18 Basf Se Método para recuperar uma proteína de interesse de um caldo de fermentação bacteriana, e, uso de pelo menos um floculante
CN112500471B (zh) * 2020-12-22 2022-09-27 中美华世通生物医药科技(武汉)股份有限公司 一种制备高澄清度蛋白琥珀酸铁的方法
EP4565710A1 (en) 2022-08-02 2025-06-11 Basf Se Stabilized protein production process using bacillus host cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3795586A (en) * 1969-08-11 1974-03-05 Pabst Brewing Co Recovery of enzymes
PL270368A1 (en) * 1987-01-30 1988-10-13 Int Minerals & Chem Corp Method for recovery of somatotropins from diluted water solutions
US4894444A (en) * 1987-07-16 1990-01-16 Merck & Co., Inc. Recovery and purification of immunogens from immunogen-alum complexes
DE3915277A1 (de) * 1989-05-10 1990-11-15 Henkel Kgaa Verfahren zur reinigung von enzymfluessigkonzentraten
DE4037530A1 (de) * 1990-11-26 1992-05-27 Henkel Kgaa Faellungsverfahren fuer exocellulaere proteine
DK91092D0 (enExample) * 1992-07-10 1992-07-10 Novo Nordisk As

Also Published As

Publication number Publication date
EP0828753B1 (en) 2003-03-26
AU5996996A (en) 1996-12-18
IN181859B (enExample) 1998-10-03
DK0828753T3 (da) 2003-06-30
DE69626976D1 (de) 2003-04-30
JPH11511118A (ja) 1999-09-28
ATE235506T1 (de) 2003-04-15
WO1996038469A1 (en) 1996-12-05
CN1186498A (zh) 1998-07-01
EP0828753A1 (en) 1998-03-18
DE69626976T2 (de) 2004-03-04

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Applicant before: Novo Nordisk A/S

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Termination date: 20120531