CN111436337A - Application of rapamycin target protein activator MHY1485 in promotion of tomato seed germination and seedling growth and development - Google Patents

Abstract

The invention discloses an application of a rapamycin target protein activator MHY1485 in promoting tomato seed germination and seedling growth and development, which is to directly purchase 10mol ∙ m L^‑1DMSO solution, MHY1485 reagent purchased, then MHY1485 dissolved in DMSO solvent to form self-prepared stock; then adding the mother solution into the prepared MS solution, and fixing the volume to a final concentration of 1-10 mu M culture solution; then thermally shocking tomato seeds in 55 deg.C water for 5min, dropping the seeds into the prepared culture solution, measuring germination rate of tomato seeds, etc., and waiting until artificial climateWhen the germination rate of the seeds in the box exceeds 80 percent, the germinated seeds can be transferred to a hole tray and treated by MHY 1485; after the seedlings grow in the plug, field planting is carried out, and MHY1485 is used as a plant inducer, so that the germination rate of tomato seeds is effectively improved, the germination time is shortened, the growth and development of the tomato seedlings are promoted, high-quality strong seedlings are formed, the survival rate of the seedlings is greatly improved, and the planting cost is reduced.

Description

Application of rapamycin target protein activator MHY1485 in promotion of tomato seed germination and seedling growth and development

Technical Field

The invention relates to a new application of a rapamycin target protein activator MHY1485, in particular to an application of the rapamycin target protein activator MHY1485 in promoting tomato seed germination and seedling growth and development.

Background

The tomato is an economic crop widely planted in the world, is rich in variety, has certain medical value and is known as 'plant gold'. In recent years, more and more farmers have tomatoes as main cultivated crops.

At present, facility tomato production in winter and spring in north is often influenced by low temperature and weak light, the disease is serious, and the growth and development of the tomatoes are inhibited. In order to ensure strong seedlings and the yield and quality of tomatoes in production, chemical fertilizers, pesticides and the like are commonly applied. However, the use of chemical fertilizers can affect the soil, and pesticide residues can cause harm to human bodies. Some producers try biological control, but different biological control agents have different effects, many biological control agents are mixtures, the effective ratio of each component to strong tomato seedlings cannot be known in detail, and the biological mechanism is not clear, so that the problems of residues, difficult degradation, whether the components are accumulated in tomatoes to be harmful to human bodies and the like still exist when the biological control agent is applied. Therefore, the promoter which is thoroughly researched, harmless to human body, easy to decompose and obvious in effect when applied in trace amount is actively searched, and the promoter has important significance and wide application prospect for the production of tomatoes.

MHY1485 is a cellular permeable rapamycin target protein TOR activator that is readily degradable at normal temperature but is highly efficient, targeting the ATP domain of TOR and functioning. The TOR is a conservative kinase for regulating cell cycle, controlling protein synthesis, guiding cell material/energy metabolism and other pathways in eukaryotes, and is a focus of researching growth and development. However, the TOR activator, MHY1485, has not been reported in the aspect of promoting plant growth and development.

Disclosure of Invention

The technical task of the invention is to provide the application of a target protein activator MHY1485 of rapamycin in promoting the germination of tomato seeds and the growth and development of seedlings in order to overcome the defects of the prior art.

The technical scheme adopted by the invention for solving the technical problems is as follows: application of target protein activator MHY1485 of rapamycin in promoting germination of tomato seed and growth and development of seedling is disclosed.

An application method of a rapamycin target protein activator MHY1485 in promoting tomato seed germination and seedling growth and development comprises the following specific steps:

a, preparing mother liquor by self, purchasing 10mol of ∙ m L^-1A DMSO solution, then dissolving MHY1485 reagent in the DMSO solvent to form a self-prepared mother liquor;

b: preparing a culture solution, namely preparing a conventional MS solution, adding the self-prepared mother solution obtained in the step A into the prepared MS solution, and fixing the volume to the culture solution with the final concentration of 1-10 mu M;

c: germinating, namely thermally shocking tomato seeds in water at 55 ℃ for 5min, then dropping the seeds into a prepared culture solution, culturing in a 18-25 ℃ artificial climate box in the dark, and measuring indexes such as seed germination rate and the like;

d: seedling raising, namely transferring the germinated seeds to a plug tray for seedling raising when the germination rate of the seeds in the artificial climate box exceeds 80%;

e: transplanting, and planting after the seedlings grow out in the plug tray.

The invention has the advantages that: the research uses MHY1485 as a plant inducer for the first time, and the plant inducer is applied to effectively improve the germination rate of tomato seeds, shorten the germination time, promote the growth and development of tomato seedlings, can form high-quality strong seedlings, greatly improves the survival rate of the seedlings and reduces the planting cost. The research shows that MHY1485 can obviously improve the germination rate of tomato seeds, promote the growth and development of tomato seedlings, provide an important theoretical basis for the research of tomato inducers, provide guidance for actual production and have wide market prospect.

Drawings

FIG. 1 shows the germination and greening at 8d for the blank and MHY1485 control test.

FIG. 2 shows the 8d root length and hypocotyl length of blank and MHY1485 control.

Detailed Description

The present invention is described in detail below with reference to the attached tables and drawings.

As shown in tables 1 and 2 and figures 1 and 2, the application of a rapamycin target protein activator MHY1485 in promoting germination and growth development of tomato seeds is disclosed, wherein the concentration of MHY1485 in MS solution is 1-10 mu M.

The method comprises the following specific steps:

example one

A, preparing mother liquor by self, and directly purchasing 10mol of ∙ m L^-1A DMSO solution, then dissolving MHY1485 reagent in the DMSO solvent to form a self-prepared mother liquor;

b: preparing a culture solution, namely preparing a conventional MS solution, adding the mother solution obtained in the step A into the prepared MS solution, and fixing the volume to the culture solution with the final concentration of 1 mu M;

c: germinating, thermally shocking the seeds in 55 deg.C water for 5min, dropping the seeds into the prepared culture solution, culturing in 20 deg.C artificial climate box in dark, and measuring seed germination rate;

d: seedling culture, wherein when the germination rate of seeds in the artificial climate box exceeds 80 percent, the germinated seeds are transferred to a plug tray for seedling culture;

e: transplanting, and planting after the seedlings grow out in the plug tray.

Example two

A, preparing mother liquor by self, and directly purchasing 10mol of ∙ m L^-1A DMSO solution, then MHY1485 is dissolved in DMSO solvent to form a self-prepared mother liquor;

b: preparing a culture solution, namely preparing a conventional MS solution, adding the mother solution obtained in the step A into the prepared MS solution, and fixing the volume to the culture solution with the final concentration of 5 mu M;

c: germinating, thermally shocking tomato seeds in 55 deg.C water for 5min, dropping the seeds into the prepared culture solution, culturing in 20 deg.C artificial climate box in dark, and measuring seed germination rate;

d: seedling culture, wherein when the germination rate of seeds in the artificial climate box exceeds 80 percent, the germinated seeds are transferred to a plug tray for seedling culture;

e: transplanting, and planting after the seedlings grow out in the plug tray.

EXAMPLE III

A, preparing mother liquor by self, purchasing 10mol of ∙ m L^-1A DMSO solution, then MHY1485 is dissolved in DMSO solvent to form a self-prepared mother liquor;

b: preparing a culture solution, namely preparing a conventional MS solution, adding the mother solution obtained in the step A into the prepared MS solution, and fixing the volume to the culture solution with the final concentration of 10 mu M;

c: germinating, thermally shocking tomato seeds in 55 deg.C water for 5min, dropping the seeds into the prepared culture solution, culturing in 20 deg.C artificial climate box in dark, and measuring seed germination rate;

d: seedling culture, wherein when the germination rate of seeds in the artificial climate box exceeds 80 percent, the germinated seeds are transferred to a plug tray for seedling culture;

e: transplanting, and planting after the seedlings grow out in the plug tray.

Table 1 blank and conditions of germination percentage of control test with addition of MHY1485 for 0-4 days

Table 2 blank and conditions of greening rate, root length, hypocotyl length, fresh weight of 8d of control test added with MHY1485

As can be seen by combining the tables 1 and 2 and the attached figures 1 and 2, under the action of the MHY1485, the germination rate of the tomatoes is obviously improved, and the greening rate, the root length, the hypocotyl length and the fresh weight after germination are obviously improved, so that the MHY1485 has a promoting effect on the germination of tomato seeds and the growth and development of seedlings.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (2)

1. Application of target protein activator MHY1485 of rapamycin in promoting germination of tomato seed and growth and development of seedling is disclosed.

2. An application method of a rapamycin target protein activator MHY1485 in promoting tomato seed germination and seedling growth and development comprises the following specific steps:

a, preparing mother liquor by self, dissolving MHY1485 reagent in 10mol of ∙ m L^-1Forming self-prepared mother liquor in DMSO solvent;

b: preparing a culture solution, namely preparing a conventional MS solution, adding the self-prepared mother solution obtained in the step A into the prepared MS solution, and fixing the volume to the culture solution with the final concentration of 1-10 mu M;

c: germinating, namely thermally shocking tomato seeds in water at 55 ℃ for 5min, then dropping the seeds into a prepared culture solution, and culturing in a 18-25 ℃ artificial climate box in the dark;

d: seedling raising, namely transferring the germinated seeds to a plug tray for seedling raising when the germination rate of the seeds in the artificial climate box exceeds 80%;

e: transplanting, and planting after the seedlings grow out in the plug tray.

Images