CN111419755B

CN111419755B - Agilawood fullerene essence and preparation method thereof

Info

Publication number: CN111419755B
Application number: CN202010361692.8A
Authority: CN
Inventors: 王美蕾; 苏永业; 凌瑞儒
Original assignee: Haikou Nanzhixiang Industry Co ltd
Current assignee: Nanzhi Guangzhou Health Technology Co ltd
Priority date: 2020-04-30
Filing date: 2020-04-30
Publication date: 2021-09-21
Anticipated expiration: 2040-04-30
Also published as: CN111419755A

Abstract

The invention provides agilawood fullerene essence and a preparation method thereof, wherein the essence mainly comprises various important amino acids such as butanediol, propylene glycol, fullerene, tyrosine and the like, and components such as agilawood extract, centella asiatica root extract, polygonum cuspidatum root extract, scutellaria baicalensis root extract, tea extract, glycyrrhiza glabra root extract, chamomile flower extract, rosemary leaf extract and the like. The essence disclosed by the invention contains various nutritional and moisturizing components such as various amino acids, fullerene, agilawood extract and the like, can moisturize and moisturize the skin for a long time, tighten the skin, and slow down and improve the generation of wrinkles and fine lines; the skin is brightened, and is tender, smooth and fine, so that the dry and rough skin becomes moist, soft and bright.

Description

Agilawood fullerene essence and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, and particularly relates to agilawood fullerene essence and a preparation method thereof.

Background

With the development of social economy and the improvement of the living standard of people, skin-care cosmetics become one of indispensable daily necessities of people. Essence is also called beauty lotion, essence lotion, etc., and is one of facial skin care products. The essence contains various functional components such as plant extracts, ceramide, squalene and the like, and mainly has the effects of moisturizing, whitening, removing freckles, resisting wrinkles and the like. Dry skin generally has poor elasticity and wrinkles deepen, so moisturizing is the basis for smoothing wrinkles and delaying aging. The existing essence products on the market are various in types and various in effects, but the effects of moisturizing and the like of the products are not obvious, and some products even can not achieve the claimed effects of moisturizing, whitening, anti-wrinkle and the like.

Disclosure of Invention

In view of the defects of the prior art, the invention provides agilawood fullerene essence and a preparation method thereof.

The scheme of the invention comprises the following aspects:

the agilawood fullerene essence comprises the following raw materials in percentage by mass:

wherein the mass ratio of valine, leucine, isoleucine, threonine, methionine, lysine, tryptophan, glycine, alanine, arginine, cystine, proline, serine, tyrosine, aspartic acid, histidine, glycerol, sodium lactate, potassium hydroxide, citric acid, monosodium citrate and pyrrolidone carboxylic acid is (0.3-0.4), (1-2), (0.7-1.0), (0.3-0.5), (2-3), (1-2), (0.5-0.8), (0.6-1), (1-2), (0.3-0.5), (2-3), (1-2), (0.5-0.8), (2-2), (1-2), (0.5-0.8), (0.6-0.6) and (1-0.4).

The mass ratio of the asiatic centella root extract to the giant knotweed rhizome extract to the baical skullcap root extract to the tea leaf extract to the glabrous root extract to the chamomile flower extract to the rosemary leaf extract is 1: (2 to 3), (0.5 to 0.8), (5 to 7), (0.6 to 1), (1 to 2).

The mass ratio of taurine, glutathione, tranexamic acid, potassium methoxysalicylate, hydroxyethyl piperazine ethane sulfonic acid and nonanodiamide MEA is 1 (6-7), (0.5-0.8), (2-3), (1-2) and (2-3).

Preferably, the agilawood fullerene essence comprises the following raw materials in percentage by mass:

wherein the mass ratio of valine, leucine, isoleucine, threonine, methionine, lysine, tryptophan, glycine, alanine, arginine, cystine, proline, serine, tyrosine, aspartic acid, histidine, glycerol, sodium lactate, potassium hydroxide, citric acid, monosodium citrate and pyrrolidone carboxylic acid is 1:0.4:2:1.0:0.5:3: 2.6: 0.6:0.8:1:2:0.5:3:2:0.8:3: 0.8:1: 0.8:0.6:1: 3;

the mass ratio of the asiatic centella root extract to the giant knotweed rhizome extract to the baical skullcap root extract to the tea leaf extract to the glabrous root extract to the chamomile flower extract to the rosemary leaf extract is 1:3:0.8:0.8:6:0.8: 2;

the mass ratio of taurine, glutathione, tranexamic acid, potassium methoxysalicylate, hydroxyethyl piperazine ethane sulfonic acid and azelaic diamide MEA is 1:7:0.8:2:2: 2.

The invention provides a preparation method of agilawood fullerene essence, which comprises the following steps:

1) dividing the raw materials into a material A, a material B, a material C, a material D and a material F;

a material: butanediol, propylene glycol, hydrolyzed sclerotium rolfsii gum, xanthan gum, sodium hyaluronate, allantoin and trehalose;

b, material B: p-hydroxyacetophenone and 1, 2-hexanediol;

c, material C: propylene glycol, valine, leucine, isoleucine, threonine, methionine, lysine, tryptophan, glycine, alanine, arginine, cystine, proline, serine, tyrosine, aspartic acid, histidine, glycerol, sodium lactate, potassium hydroxide, citric acid, monosodium citrate, pyrrolidone carboxylic acid, nicotinamide, centella asiatica root extract, polygonum cuspidatum root extract, scutellaria baicalensis root extract, tea leaf extract, glycyrrhiza glabra root extract, matricaria chamomilla flower extract, and rosemary leaf extract;

d, material: taurine, glutathione, tranexamic acid, potassium methoxysalicylate, hydroxyethyl piperazine ethanesulfonic acid, azelaic diamide MEA and Chinese eaglewood extract;

f, material: fullerene, beta-glucan, ceramide 1, and hexapeptide-1.

2) Heating water to 85 +/-5 ℃, adding the material A, and stirring and dissolving at 100-200 rpm for 15-20 minutes;

3) cooling the product obtained in the step 2) to 45 +/-2 ℃, sequentially adding the material B, the material C, the material D and the material F, mixing and stirring for 16-20 minutes at 100-200 rpm, filtering, inspecting, filling, packaging, inspecting and warehousing.

The invention further provides another preparation method of the agilawood fullerene essence, which comprises the following steps of:

b, material B: p-hydroxyacetophenone and 1, 2-hexanediol;

f, material: fullerene, beta-glucan, ceramide 1, and hexapeptide-1.

2) Uniformly mixing the material A, and stirring at 50-75 rpm for 15-20 min at 70 +/-5 ℃;

3) heating water to 85 +/-5 ℃, adding the material C, stirring and dissolving at 100-200 rpm for 15-20 minutes, and adjusting the pH to 7.3-7.8;

4) slowly adding the material obtained in the step 3) into the material obtained in the step 2) at 55 +/-5 ℃, uniformly mixing, carrying out high-pressure homogenization for 3-4 times at the homogenization pressure of 600-800 bar, cooling the material to room temperature, sequentially adding the material B, the material D and the material F, mixing and stirring at 100-200 rpm for 16-20 minutes, filtering, inspecting, filling, packaging, inspecting and warehousing.

Preferably, the pH is 7.6.

Preferably, the homogenization pressure is 600 bar.

Preferably, the mass ratio of the material A to the material C is 1:1.

compared with the prior art, the invention has the beneficial effects that:

the essence disclosed by the invention contains various nutritional and moisturizing components such as amino acids, fullerene, agilawood extract and the like, can moisturize and moisturize skin for a long time, tighten the skin and slow down and improve the generation of wrinkles and fine lines; the skin is brightened, and is tender, smooth and fine, so that the dry and rough skin becomes moist, soft and bright.

The invention also discovers that the photodegradation rate of amino acid components such as tyrosine and tryptophan can be remarkably relieved and the stability of the essence disclosed by the invention is improved by improving the preparation method on the basis of the existing formula.

Detailed Description

In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.

The raw materials used by the facial cleanser are all materials which are commercially available and meet the standards of raw materials of cosmetics.

Examples 1 to 3

The agilawood fullerene essence is characterized by comprising the following raw materials in percentage by mass:

Example 4

Example 4 differences from example 1:

valine, leucine, isoleucine, threonine, methionine, lysine, tryptophan, glycine, alanine, arginine, cystine, proline, serine, tyrosine, aspartic acid, histidine, glycerol, sodium lactate, potassium hydroxide, citric acid, monosodium citrate, and pyrrolidone carboxylic acid in a mass ratio of 1:0.4: 1.0:0.5:3:1:0.8:0.8:0.8:0.6:2:0.3:2:1:0.5:2:1:0.8:1:1: 3.

The mass ratio of the asiatic centella root extract to the giant knotweed rhizome root extract to the baical skullcap root extract to the tea leaf extract to the glabrous root extract to the chamomile flower extract to the rosemary leaf extract is 1:3:0.8:0.8:5:1: 1.

The mass ratio of taurine, glutathione, tranexamic acid, potassium methoxysalicylate, hydroxyethyl piperazine ethane sulfonic acid and azelaic diamide MEA is 1:6:0.5:2:1: 2.

Example 5

Example 5 differences from example 1:

valine, leucine, isoleucine, threonine, methionine, lysine, tryptophan, glycine, alanine, arginine, cystine, proline, serine, tyrosine, aspartic acid, histidine, glycerol, sodium lactate, potassium hydroxide, citric acid, monosodium citrate, and pyrrolidone carboxylic acid in a mass ratio of 1:0.3: 2.7: 0.3:2:2:0.5:0.5:0.5:1: 0.5:3:2:0.8:3:2:0.5:0.6:0.6: 2.

The mass ratio of the asiatic centella root extract to the giant knotweed rhizome extract to the baical skullcap root extract to the tea leaf extract to the glabrous root extract to the chamomile flower extract to the rosemary leaf extract is 1: 2:0.5:0.5:7:0.6:2.

The mass ratio of taurine, glutathione, tranexamic acid, potassium methoxysalicylate, hydroxyethyl piperazine ethane sulfonic acid and azelaic diamide MEA is 1:7:0.8:3:2: 3.

The preparation method of examples 1 to 4 was:

a material: butanediol, propylene glycol with half formula amount, hydrolyzed sclerotium rolfsii gum, xanthan gum, sodium hyaluronate, allantoin and trehalose;

b, material B: p-hydroxyacetophenone and 1, 2-hexanediol;

c, material C: half formula amounts of propylene glycol, valine, leucine, isoleucine, threonine, methionine, lysine, tryptophan, glycine, alanine, arginine, cystine, proline, serine, tyrosine, aspartic acid, histidine, glycerol, sodium lactate, potassium hydroxide, citric acid, monosodium citrate, pyrrolidone carboxylic acid, nicotinamide, centella asiatica root extract, polygonum cuspidatum root extract, scutellaria baicalensis root extract, tea leaf extract, glycyrrhiza glabra root extract, matricaria chamomilla flower extract, and rosemary leaf extract;

f, material: fullerene, beta-glucan, ceramide 1, and hexapeptide-1.

2) Heating water to 85 +/-5 ℃, adding the material A, and stirring and dissolving for 15 minutes at 200 rpm;

3) cooling the product obtained in the step 2) to 45 +/-2 ℃, sequentially adding the material B, the material C, the material D and the material F, mixing and stirring for 16 minutes at 200rpm, filtering, inspecting, filling, packaging, inspecting and warehousing.

The preparation method of example 5 is as follows:

b, material B: p-hydroxyacetophenone and 1, 2-hexanediol;

f, material: fullerene, beta-glucan, ceramide 1, and hexapeptide-1.

2) Heating water to 85 +/-5 ℃, adding the material A, and stirring at 100rpm for dissolving for 20 minutes;

3) cooling the product obtained in the step 2) to 45 +/-2 ℃, sequentially adding the material B, the material C, the material D and the material F, mixing and stirring for 20 minutes at 100rpm, filtering, inspecting, filling, packaging, inspecting and warehousing.

Example 6

The main differences between example 6 and example 1 are:

the preparation method of the agilawood fullerene essence comprises the following steps:

b, material B: p-hydroxyacetophenone and 1, 2-hexanediol;

f, material: fullerene, beta-glucan, ceramide 1, and hexapeptide-1.

2) Mixing the material A uniformly, and stirring at 50rpm at 70 +/-5 ℃ for 20 min;

3) heating water to 85 +/-5 ℃, adding the material C, stirring at 100rpm for dissolving for 20 minutes, and keeping the pH value at 7.6;

4) slowly adding the material obtained in the step 3) into the material obtained in the step 2) at the temperature of 55 +/-5 ℃, uniformly mixing, carrying out high-pressure homogenization for 4 times, wherein the homogenization pressure is 600bar, cooling the material to room temperature, sequentially adding the material B, the material D and the material F, mixing and stirring at 100rpm for 20 minutes, filtering, inspecting, filling, packaging, inspecting and warehousing.

Example 7

The main differences between example 7 and example 1 are:

b, material B: p-hydroxyacetophenone and 1, 2-hexanediol;

f, material: fullerene, beta-glucan, ceramide 1, and hexapeptide-1.

2) Mixing the material A uniformly, and stirring at 75rpm at 70 +/-5 ℃ for 15 min;

3) heating water to 85 +/-5 ℃, adding the material C, stirring at 200rpm for dissolving for 15 minutes, and keeping the pH value at 7.3;

4) slowly adding the material obtained in the step 3) into the material obtained in the step 2) at the temperature of 55 +/-5 ℃, uniformly mixing, carrying out high-pressure homogenization for 3 times, wherein the homogenization pressure is 800bar, cooling the material to room temperature, sequentially adding the material B, the material D and the material F, mixing and stirring at 200rpm for 16 minutes, filtering, inspecting, filling, packaging, inspecting and warehousing.

Comparative example 1

The main differences between comparative example 1 and example 1 are:

valine, leucine, isoleucine, threonine, methionine, lysine, tryptophan, glycine, alanine, arginine, cystine, proline, serine, tyrosine, aspartic acid, histidine, glycerol, sodium lactate, potassium hydroxide, citric acid, monosodium citrate, and pyrrolidone carboxylic acid in a mass ratio of 1:1:3:2:5:2:1:2:0.3:0.8:0.5:2:3:1:1: 2:2:0.5:0.5:0.6: 1.

The mass ratio of the asiatic centella root extract to the giant knotweed rhizome extract to the baical skullcap root extract to the tea leaf extract to the glabrous root extract to the chamomile flower extract to the rosemary leaf extract is 1:2:1:0.8:3:0.5: 3.

Experimental example 1:

the moisture retention and water supplement capability of the reaction product is measured by measuring the water content of the cuticle of the epidermis in the experiment.

The experimental method comprises the following steps: healthy women aged 35-40 years were selected and subjects received no skin beauty, skin treatment, and other tests that may affect outcome within 1 year. The test environment temperature is 25 +/-2 ℃, the relative humidity is 60 +/-5%, the moisture content of the horny layer of the epidermis at the cheek part is detected by SKICON-200 before the product is used, then the samples of each embodiment and comparative example are recommended to be used in groups, each group of 10 people uses the product for 1 time each day, the moisture content of the horny layer of the epidermis is detected again after the product is continuously used for 1 month, and the change rate of the moisture content of the horny layer of the epidermis is counted. The results are shown in Table 1.

TABLE 1

	Before use (us)	After use (us)	Rate of change (%)
				Example 1	229.3±5.23	269.4±5.34	17.49％
Example 2	225.5±6.12	266.5±6.23	18.18％
				Example 3	219.1±3.78	280.1±4.23	27.84％
Example 4	230.3±7.12	267.2±5.22	16.02％
				Example 5	233.8±4.24	278.8±4.45	19.25％
Example 6	218.9±5.23	258.9±7.34	18.27％
				Example 7	226.7±3.67	269.7±5.32	18.97％
Comparative example 1	229.4±5.32	255.3±5.09	11.29％

The result shows that the moisture content of the horny layer of the epidermis of the skin is obviously improved after the essence disclosed by the invention is used, and the formula disclosed by the invention has good moisturizing and hydrating effects.

Experimental example 2:

and (4) determining the content of the amino acid by adopting a high performance liquid chromatography.

A sample to be tested: taking 1mL of sample to be tested, diluting to 20mL with formic acid aqueous solution with pH of 8, performing ultrasonic extraction for 20min, centrifuging at 8000rpm, taking supernatant, filtering with a filter membrane, and taking filtrate for later use.

Blank sample: in each of examples and comparative examples, the sample to which the component to be measured was not added was prepared in the same manner as the test sample.

Illuminating the sample: 50ml of each sample and blank sample of each example are respectively placed in a transparent glass bottle, placed under 8000lx illumination for 8h, and then sampled and prepared according to the method.

Chromatographic conditions are as follows: diamonsil C18 column (200 mm. times.4.6 mm, 5 μm); the flow rate is 0.5 mL/min; the column temperature is 30 ℃; the sample volume is 25 mu L; mobile phase: v (acetonitrile): V (5mmol/L aqueous formic acid) ═ 10: 90; the detection wavelength is 280 nm.

4 batches were produced for each sample tested, and the average was taken 2 times for each batch. The decomposition rate was calculated as peak area and corrected as blank. The results are shown in Table 2. The components to be tested in this experiment were tyrosine and tryptophan.

The decomposition rate (area of peak of sample not illuminated-area of peak of illuminated sample)/area of peak of sample not illuminated.

TABLE 2

Note: the different letters in each column represent significant differences (p <0.05)

The results show that:

by inclusion-treating amino acid components such as tyrosine and tryptophan by the preparation methods of example 6 and example 7, the decomposition rate of components such as tyrosine and tryptophan under light conditions is avoided, and the stability of the amino acid components is further improved. The selection of the amounts of the ingredients to be dosed also has an influence on the decomposition rates of tyrosine and tryptophan.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. The agilawood fullerene essence is characterized by comprising the following raw materials in percentage by mass:

wherein, the mass ratio of valine, leucine, isoleucine, threonine, methionine, lysine, tryptophan, glycine, alanine, arginine, cystine, proline, serine, tyrosine, aspartic acid, histidine, glycerol, sodium lactate, potassium hydroxide, citric acid, monosodium citrate and pyrrolidone carboxylic acid is 1 (0.3-0.4), (1-2), (0.7-1.0), (0.3-0.5), (2-3), (1-2), (0.5-0.8), (0.6-1), (1-2), (0.3-0.5), (2-3), (1-2), (0.5-0.8), (2-2), (1-2), (0.5-0.8), (0.6-0.6) and (1-0.4);

the mass ratio of the asiatic centella root extract to the giant knotweed rhizome extract to the baical skullcap root extract to the tea leaf extract to the glabrous root extract to the chamomile flower extract to the rosemary leaf extract is 1: (2 to 3), (0.5 to 0.8), (5 to 7), (0.6 to 1), (1 to 2);

the mass ratio of taurine, glutathione, tranexamic acid, potassium methoxysalicylate, hydroxyethyl piperazine ethane sulfonic acid and nonanodiamide MEA is 1 (6-7), (0.5-0.8), (2-3), (1-2) and (2-3);

b, material B: p-hydroxyacetophenone and 1, 2-hexanediol;

f, material: fullerene, beta-glucan, ceramide 1, and hexapeptide-1;

2. The agilawood fullerene essence according to claim 1, which comprises the following raw materials in percentage by mass:

3. The agilawood fullerene essence according to claim 1, wherein the pH is 7.6.

4. The agilawood fullerene essence according to claim 1, wherein the homogenization pressure is 600 bar.

5. The agilawood fullerene essence according to claim 1, wherein the mass ratio of the material A to the material C is 1:1.