CN111388371A

CN111388371A - Anti-hair loss hair-strengthening hair-growing liquid composition containing fibroin and preparation method thereof

Info

Publication number: CN111388371A
Application number: CN202010261130.6A
Authority: CN
Inventors: 张向阳; 甘景苗
Original assignee: Guangzhou Qiyuan Biotechnology Co ltd
Current assignee: Guangzhou Qiyuan Biotechnology Co ltd
Priority date: 2020-04-03
Filing date: 2020-04-03
Publication date: 2020-07-10

Abstract

The invention discloses a hair-fixing and hair-loss-preventing hair-growing liquid composition containing silk protein and a preparation method thereof, wherein the hair-growing liquid composition comprises raw material components of 95.6-78.1% of deionized water, 1.0-3.0% of butanediol, 0.01-0.1% of sodium hyaluronate, 0.01-0.1% of EDTA.2Na, 0.01-0.2% of allantoin, 0.1-1.0% of D-panthenol, 0.1-0.5% of α -bisabolol, 0.1-1.0% of hydrogenated castor oil, 0.03-1.0% of citric acid, 3.0-5.0% of silk protein liquid, 0-10% of traditional Chinese medicine extract and proper amounts of preservative and essence.

Description

Anti-hair loss hair-strengthening hair-growing liquid composition containing fibroin and preparation method thereof

Technical Field

The invention relates to a hair-fixing and hair-loss-preventing composition, belongs to the technical field of hair-growing and hair-nourishing products, and particularly relates to a hair-fixing and hair-loss-preventing hair-growing liquid composition containing fibroin and a preparation method thereof.

Background

In the current social development, there are many influences of internal factors such as mental stress caused by deterioration of air quality, increase in living pressure, insufficient sleep in night life, and the like, and hormonal disorders in the body. More and more consumers are beginning to develop scalp problems such as scalp inflammation, hair oil, excessive hair loss and alopecia areata.

At present, hair loss prevention and hair growth promotion products on the market mainly comprise shampoo, oral medicaments and other products, most of the hair loss prevention and hair growth prevention products are pure traditional Chinese medicines, more than ten or dozens of traditional Chinese medicines are matched, the components are complex, or western medicine components are added, certain irritation and high risk are brought to scalp, and the hair loss prevention and hair growth prevention effects are not obvious.

Therefore, it is necessary to develop a hair-fixing and hair-loss-preventing hair-growing liquid which is efficient, safe and free of side effects.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide a hair-strengthening and hair-loss-preventing hair-growing liquid composition containing fibroin, which is mainly used for improving the scalp micro-ecological environment consisting of physiological lipid, natural moisturizing factors and scalp microbial population of the scalp, maintaining the health of the scalp and promoting the activity of human fibroblasts by compounding fibroin liquid with other components, thereby really achieving the technical effects of hair growing and hair strengthening.

The invention also aims to provide a preparation method of the hair-fixing and hair-loss-preventing hair-growing liquid composition containing fibroin.

The purpose of the invention and the technical problem to be solved are realized by adopting the following technical scheme. The invention provides a fibroin-containing hair loss prevention, solidification and growth promotion liquid composition which comprises the following raw material components in percentage by weight:

1.0-3.0% of butanediol; 0.01 to 0.1 percent of sodium hyaluronate; 0.01 to 0.1 percent of EDTA.2Na; 0.01-0.2% of allantoin; 0.1-1.0% of D-panthenol;

α -bisabolol 0.1-0.5%, hydrogenated castor oil 0.1-1.0%;

silk protein liquid 3.0-5.0%; 0-10% of traditional Chinese medicine extract; 0.03-1.0% of citric acid; proper amount of essence and preservative, and the balance of deionized water.

Further, the content range of the traditional Chinese medicine extract in the anti-hair loss, hair-strengthening and hair-growing liquid composition containing fibroin is 0.1% -10%; a preferred range is 0.5-5%.

The traditional Chinese medicine extract is selected from one or more of 0.1-1.0% of Chinese arborvitae extract, 0.1-1.0% of polygonum multiflorum extract, 0.1-1.0% of south dodder seed extract, 0.1-1.0% of wormwood extract, 0.1-1.0% of privet extract, 0.1-1.0% of angelica sinensis extract, 0.1-1.0% of ginseng extract, 0.1-1.0% of ganoderma atrum extract, 0.1-1.0% of liquorice extract, 0.1-1.0% of citrus peel extract and 0.1-1.0% of zanthoxylum fruit extract.

Further, in the anti-hair loss and hair-growing liquid composition containing fibroin, the fibroin liquid is prepared by fresh cut cocoons by adopting the latest O-discharge double-circulation biochemical hydrolysis technology, controlling the hydrolysis degree of the silk by using hydrolytic enzyme, disorderly winding protein bundles of the silk, cutting the protein bundles into proteins with different grades and molecular weights, and combining the proteins to obtain the fibroin liquid;

the specific preparation process of the silk protein liquid comprises the following steps:

1) 0.4 to 6 mass percent of Na is used₂CO₂Or NaHCO₃The aqueous solution is used as degumming agent for degumming silkworm cocoons under the conditions that the pH value is 8-11, the hydrolysis temperature is 50-80 ℃ and the time is 2-4 hours; filtering after the degumming is finished to obtain degumming hydrolysate and silk fibroin fibers;

2) removing salt in the degumming hydrolysate through electrodialysis, obtaining sericin hydrolysate and dialysis ion solution in the process, retaining the sericin hydrolysate, and transferring the dialysis ion solution to next preparation of the sericin hydrolysate for use;

3) dissolving the silk fibroin fibers obtained by filtering in the step 1) in CaCl with the mass percent of 30-60% at the temperature of 60-100 DEG C₂、CaCl₂-H₂O、CaCl₂-2H₂Hydrolyzing in neutral salt solution O or L iBr to obtain silk fibroin solution;

4) performing electrodialysis on the hydrolyzed silk fibroin solution obtained in the step 3) to remove ions in the solution, and circulating the solution until the conductivity of the solution reaches 10-500us/cm to obtain a light yellow silk fibroin pure solution, wherein the ion solution after electrodialysis can be circularly used as a neutral salt solution in the step 4);

5) and (3) uniformly mixing the sericin hydrolysate obtained in the step (2) and the fibroin pure solution obtained in the step (4) to obtain the fibroin solution.

Further, in the above anti-hair loss, hair-strengthening and hair-growing liquid composition containing fibroin, in step 1), the method for cultivating the cut cocoon of fibroin liquid comprises:

1.1) selecting mulberry field:

mulberry trees are planted in the Yunnan mountain area with sufficient sunlight and large day-night temperature difference, and the mulberry leaves have high sugar content and are beneficial to the healthy growth of the silkworm, so that high-quality cocoon shells with thick shells and large sizes are obtained; in addition, a natural barrier is formed by the unique small basin structure in the mountainous area of Yunnan, so that the pesticide in the non-silkworm rearing area is reduced from scattering to the silkworm rearing area, and the pesticide residue of the final product is guaranteed;

1.2) selection of silkworm species:

the breeding silkworm is a second generation silkworm obtained from a silkworm industry technical station, and the breeding silkworm is adopted for the purpose of stable content of the obtained sericin protein and the silk fibroin protein, so that a hydrolysis final product with a stable ratio of the hydrolyzed sericin protein to the hydrolyzed silk fibroin protein is obtained;

1.3) culture environment:

the culture environment of the silkworm is related to the microbial control of the later-stage product and silkworm leaf disease; the silkworm breeding environment adopts aseptic, ventilated and dry silkworm breeding in a room so as to reduce the risks of germs, microorganisms and the like of a final product;

1.4) storage conditions:

silk is an active protein and needs to be stored in a cool, dry, ventilated place.

Further, in the above anti-hair loss hair-growing liquid composition containing fibroin, in step 5), the molecular weight of fibroin of the final product obtained in step 5) is controlled within an interval of 2-20 ten thousand daltons by controlling the hydrolysis end point, and is mainly concentrated within an interval of 10-20 ten thousand daltons.

Further, the anti-hair loss hair-fixing hair-growing liquid composition containing fibroin comprises the following raw material components in parts by weight:

93.7% of deionized water and 1.0% of butanediol; 0.05% of sodium hyaluronate; 0.05 percent of EDTA.2Na; 0.05% of allantoin; 0.2% of D-panthenol;

α -bisabolol 0.2%, hydrogenated castor oil 0.5%;

3.0% of silk protein liquid; 0.1% of arborvitae extract; 0.1% of polygonum multiflorum extract; 0.1% of south dodder seed extract; 0.1% of Artemisia princeps Pampanini extract; 0.1% of privet extract; 0.1% of angelica extract; 0.1% of ginseng extract; 0.1% of ganoderma atrum extract; 0.1% of licorice extract; citrus peel extract 0.1%; 0.1% of zanthoxylum bungeanum fruit extract; 0.03 percent of citric acid; a proper amount of essence; proper amount of preservative.

79.3 percent of deionized water; 3.0% of butanediol; 0.05% of sodium hyaluronate; 0.1% of EDTA.2Na; 0.05% of allantoin; 0.5 percent of D-panthenol;

α -bisabolol 0.03-0.1%, hydrogenated castor oil 0.5%;

4.0% of silk protein liquid; 1.0% of arborvitae extract; 1.0% of polygonum multiflorum extract; south dodder seed extract 1.0%; 1.0% of artemisia scoparia extract; 1.0% of privet extract; 1.0% of angelica sinensis extract; 1.0% of ginseng extract; 1.0% of ganoderma atrum extract; 1.0% of liquorice extract; 1.0% of citrus peel extract; 1.0% of zanthoxylum bungeanum fruit extract; 0.03 percent of citric acid; an essence; proper amount of preservative.

In addition, the invention also discloses a preparation method of the anti-hair loss hair-fixing hair-growing liquid composition containing fibroin, which comprises the following steps:

1) firstly, stirring, mixing and dissolving deionized water, butanediol and sodium hyaluronate completely, adding EDTA-2Na, allantoin and D-panthenol after the materials are dissolved completely, stirring and dissolving completely at the temperature of 80-85 ℃, and mixing a material body A;

2) after the mixture A in the step 1) is completely dissolved, α -bisabolol and hydrogenated castor oil are sequentially mixed, stirred and dissolved evenly to prepare a mixture B at the temperature of about 60-70 ℃;

3) after the temperature is reduced to about 50-60 ℃, proper amounts of traditional Chinese medicine extract, fibroin liquid, citric acid, essence and preservative are sequentially added into the mixture body B in the step 2) and are uniformly stirred and mixed, and then the material is discharged, so that the anti-hair loss hair-strengthening hair-growing liquid composition is prepared.

Further, the preparation method of the anti-hair loss, hair-fixing and hair-growing liquid composition containing fibroin comprises the following steps:

the anti-hair loss hair-strengthening hair-growing liquid composition containing fibroin is prepared from the following components in percentage by weight:

the component A comprises the following components: 93.1% of deionized water; 2.0% of butanediol; 0.05% of sodium hyaluronate; 0.05 percent of EDTA.2Na; 0.01 percent of allantoin; 0.2% of D-panthenol;

the component B comprises α -bisabolol 0.2%, and hydrogenated castor oil 0.2%;

c phase component: 3.0% of silk protein liquid; 0.1% of arborvitae extract; 0.1% of polygonum multiflorum extract; 0.1% of south dodder seed extract; 0.1% of Artemisia princeps Pampanini extract; 0.1% of privet extract; 0.1% of angelica extract; 0.1% of ginseng extract; 0.1% of ganoderma atrum extract; 0.1% of licorice extract; citrus peel extract 0.1%; 0.1% of zanthoxylum bungeanum fruit extract; 0.03 percent of citric acid; a proper amount of essence; proper amount of preservative.

The preparation method of the anti-hair loss hair-fixing hair-growing liquid composition containing fibroin comprises the following steps;

1) stirring, mixing and dissolving the phase A completely at the temperature of about 80-85 ℃, and preserving heat for half an hour;

2) adding B after the A phase is completely dissolved, stirring and dissolving completely at 60-70 deg.C

3) And after the temperature is reduced to about 50-60 ℃, adding the phase C into the material body, stirring and mixing uniformly, and discharging.

The source and the effect of the raw material components of the invention are explained as follows:

① fibroin liquid:

the fibroin liquid is a small and medium molecular fibroin hydrolyzed mixed liquid obtained by using fresh cut cocoons and adopting a 0-discharge twice circulating hydrolysis process. The invention adopts the latest biochemical technology, controls the hydrolysis degree of silk by hydrolase, disorderly twines the protein bundles of the silk, cuts the protein bundles into proteins with different grades and molecular weights, and combines the proteins to obtain the silk protein liquid. The protein molecule has surface activity, can increase the foam stability of a system, prolong the foam retention time of a compound system, and simultaneously can reduce the irritation of other surfactants. The fibroin solution contains keratin, small molecular peptides, amino acids and other parts which can be absorbed and utilized by scalp, certain nutrient components can be supplemented, damaged hair scales can be effectively repaired, and release of inflammatory substances can be inhibited, so that the fibroin solution has a certain itching relieving effect, and meanwhile, the keratin can meet the nutrition required by cell growth, promote the activity of human skin fibroblasts, and is beneficial to hair growth and hair strengthening.

② phase A component

The butanediol in the phase A component is mainly used as a skin conditioner in the formula, and has the efficacy of moisturizing as a solvent; the sodium hyaluronate is mainly used for moisturizing and moistening the scalp; EDTA-2Na is a metal chelating agent and can chelate metal ions in the product; allantoin has the functions of promoting the generation of skin cells, quickly healing wounds, being a good healing agent for skin wounds, softening keratin and keeping the skin moist, moist and soft.

④ B phase component

The hydrogenated castor oil ester in the component B mainly has the effect of increasing the solvent, mainly increases the solubility of α -bisabolol in the formula, and α -bisabolol has excellent effects of resisting inflammation, diminishing inflammation, sterilizing, relieving itching, promoting cell regeneration, promoting skin metabolism and the like, and has a good repairing effect on the scalp inflammation.

⑤ Chinese medicinal extract

The traditional Chinese medicine extract in the embodiment of the invention is an optional component, and adopts a traditional Chinese medicine extracting solution, wherein the oriental arborvitae extract mainly has the effects of growing and blackening hair and improving the production environment of scalp so as to prevent hair loss and fix hair, the fleece-flower root extracting solution mainly can promote the proliferation of B-16 melanocyte and the differentiation of colloid keratinocytes so as to have a good effect on growing and blackening hair, the south dodder extract mainly has an inhibiting effect on 5 α -reductase, the northern wormwood extract, the artemisia leaf butanediol extract, the privet extract, the water extract, the angelica sinensis extract, the ginseng extract, the tangerine peel extract, the zanthoxylum bungeanum maxim and the like can promote blood circulation and remove blood stasis, improve skin microcirculation and increase the value of capillary endothelial cells so as to provide a good help on the aspect of growing hair, the ginseng extract can promote the growth of hair, the ganoderma atrum extract can inhibit 5 α -reductase and has a promoting effect on the proliferation of hair external root sheath cells, and the extracts of liquorice extract, citrus peel extract, zanthoxylum bunge fruit and the like can activate deep hair follicle mother cells, effectively prevent hair, and promote hair growth and have an anti-inflammatory effect.

By means of the technical scheme, the invention has the following advantages and beneficial technical effects:

1) in the silk protein liquid component in the hair loss preventing, fixing and growing liquid, small molecular peptides and amino acids can be partially absorbed and utilized by the scalp, and have nourishing and nourishing effects on the scalp and the hair; macromolecular sericin and keratin can form a 'mud' cell adhesion substance on the surfaces of the scalp and the hair, and the 'mud' cell adhesion substance can repair epidermal cells to be peeled off and delay the shedding of the scalp; also can repair hair stem, reduce hair brittle fracture, bifurcation and shedding, and has the efficacy after being continuously used for one month;

2) in the hair restorer composition, the silk protein liquid is used together with other components (α -bisabolol and the like, traditional Chinese medicine extract liquid), so that the micro-ecological environment of the scalp is improved, the release of inflammatory factors is reduced, the activity of human fibroblasts is promoted, and the scales of damaged hair are effectively repaired, thereby really achieving the purposes of hair restoring and hair fixing.

3) The invention mainly improves scalp micro-ecological environment composed of physiological lipid, natural moisturizing factor and scalp microbial population through the silk protein liquid, maintains scalp health, and promotes the activity of human fibroblast, thereby achieving the purposes of hair growth and hair strengthening.

Drawings

FIGS. 1a to 1d are graphs for testing the repairing effect of silk protein liquid on hair scales;

FIG. 2 is a statistical graph showing the percentage of macrophages releasing 1L-1 a and 1L-6 caused by L PS according to the present invention for different fibroin solutions;

FIG. 3 is a histogram of the human skin fibroblast viability for different fibroin fluid concentrations of the present invention;

fig. 4 is a graph of 2D cell repair tests with fibroin fluids that can repair damaged scalp.

Detailed Description

The invention discloses a hair tonic composition which contains a silk protein liquid component, mainly comprises medium and small protein molecules with the molecular weight of 2-20 ten thousand Da (or 10-20 ten thousand Da), is rich in silk protein, peptide, amino acid and the like, and has the functions of nourishing and repairing hair, scalp and hair root.

The invention discloses a hair-fixing and hair-loss-preventing hair-growing liquid composition containing fibroin and a preparation method thereof. The hair restorer composition comprises the following main raw material components:

deionized water, butanediol, sodium hyaluronate, EDTA.2Na, allantoin, D-panthenol, α -bisabolol, hydrogenated castor oil, fibroin liquid, a proper amount of essence and a proper amount of preservative.

The fibroin liquid is used together with other components (α -bisabolol, traditional Chinese medicine extract (optional component) and the like), so that the micro-ecological environment of scalp is improved, the release of inflammatory factors is reduced, the activity of human fibroblast is promoted, and the damaged hair scales are effectively repaired, thereby really achieving the purposes of hair growth and hair fixation.

The specific process of the silk protein liquid is as follows:

the fibroin solution is prepared by fresh cut cocoons by adopting the latest O-discharge double-circulation biochemical hydrolysis technology, controlling the hydrolysis degree of silk through hydrolytic enzyme, disorderly winding protein bundles of the silk, cutting the protein bundles into proteins with different grades and molecular weights, and combining the proteins to obtain the fibroin solution. The method specifically comprises the following process steps:

2) removing salt in the degumming hydrolysate through electrodialysis, obtaining sericin hydrolysate and dialysis ion solution from the degumming hydrolysate, retaining the sericin hydrolysate, and transferring the dialysis ion solution to next preparation of the sericin hydrolysate for use;

5) uniformly mixing the sericin hydrolysate obtained in the step 2) and the fibroin pure solution obtained in the step 4) to obtain fibroin liquid; by controlling the hydrolysis end point, the molecular weight of the fibroin of the obtained final product is controlled within the range of 2-20 ten thousand daltons, and the molecular weight is mainly centralized within the range of 10-20 ten thousand daltons.

In the silk hydrolysate disclosed by the invention, small molecular peptides and amino acids can be partially absorbed and utilized by the scalp, and have nourishing and nourishing effects on the scalp and hair. The macromolecular sericin and keratin can form a cell adhesion substance like 'mud' on the surfaces of the scalp and the hair, and the cell adhesion substance can repair epidermal cells to be peeled off and delay the shedding of the scalp. Also can repair hair stem, reduce hair brittle fracture, bifurcation and shedding, and has the effect after being continuously used for one month. Meanwhile, the toughness of the hair can be enhanced. Therefore, the invention mainly adopts the silk hydrolysate to rebuild and protect the scalp and scalp microecology, thereby achieving the purposes of hair growth and hair-fixing.

The present invention is further defined by the following specific preferred embodiments, but the present invention is not limited to the following embodiments. The invention mainly adopts fibroin liquid, is added into a formula for nourishing and protecting hair to prepare a product containing fibroin with the functions of fixing hair, preventing hair loss and growing hair, and describes the process operation of the formula of the product.

Example 1

A hair-fixing and hair-loss-preventing hair-growing liquid composition containing fibroin is prepared from the following raw material components in percentage by weight:

phase A component:

92.47% of deionized water and 1.0% of butanediol; 0.05% of sodium hyaluronate; 0.05 percent of EDTA.2Na; 0.1 percent of allantoin; 0.2% of D-panthenol;

b phase component:

α -bisabolol 0.1%, hydrogenated castor oil 0.5%;

c phase component:

4.0% of silk protein liquid; 0.03 percent of citric acid; 1.0% of propylene glycol; 0.3 percent of phenoxyethanol; 0.05 percent of essence.

Example 2

phase A component:

90.97% of deionized water and 2.0% of butanediol; 0.05% of sodium hyaluronate; 0.05 percent of EDTA.2Na; 0.05% of allantoin; 0.5 percent of D-panthenol;

b phase component:

α -bisabolol 0.5%, hydrogenated castor oil 0.5%;

c phase component:

3.0% of silk protein liquid; 0.03 percent of citric acid; 1.0% of propylene glycol; 0.3 percent of phenoxyethanol; 0.05% of essence;

the anti-hair loss hair-strengthening hair-growing liquid composition containing fibroin of the above embodiment 1-2 is prepared by the following process steps:

1) firstly, stirring, mixing and dissolving the phase A completely at the temperature of about 80-85 ℃, and cooling;

Example 3

phase A component:

92.42% of deionized water and 1.0% of butanediol; 0.1% of sodium hyaluronate; 0.05 percent of EDTA.2Na; 0.05% of allantoin; 0.2% of D-panthenol;

b phase component:

α -bisabolol 0.2%, hydrogenated castor oil 0.5%;

c phase component:

3.0% of silk protein liquid; cacumen Platycladi extract 0.1%; 0.1% of polygonum multiflorum extract; 0.1% of south dodder seed extract; 0.1% of Artemisia princeps Pampanini extract; 0.1% of privet fruit extract; 0.1% of angelica root extract; 0.1% of ginseng root extract; 0.1% of kaempferia galanga root extract; licorice root extract 0.1%; citrus peel extract 0.1%; 0.1% of zanthoxylum bungeanum fruit extract; 0.03 percent of citric acid; 1.0% of propylene glycol; 0.3 percent of phenoxyethanol; 0.05 percent of essence.

Example 4

phase A component:

90.31% of deionized water and 2.0% of butanediol; 0.05% of sodium hyaluronate; 0.1% of EDTA.2Na; 0.1 percent of allantoin; 0.2% of D-panthenol;

b phase component:

α -bisabolol 0.05%, hydrogenated castor oil 0.5%;

c phase component:

4.0% of silk protein liquid; cacumen Platycladi extract 0.2%; 0.2% of polygonum multiflorum extract; 0.2% of south dodder seed extract; 0.2% of artemisia scoparia extract; glossy privet fruit extract 0.2%; 0.2% of angelica root extract; 0.2% of ginseng root extract; 0.2 percent of kaempferia root extract; licorice root extract 0.2%; citrus peel extract 0.2%; 0.2% of zanthoxylum bungeanum fruit extract; 0.04% of citric acid; 0.4 percent of phenoxyethanol; 0.05 percent of essence.

The anti-hair loss hair-fixing hair-growing liquid composition containing fibroin of the above embodiment 3-4 is prepared by the following process steps:

The following are contents of the effect test examples.

Effect experiment example 1

The product of the invention is mainly added with fibroin liquid and other components (traditional Chinese medicine extract) for matching use. In order to achieve the test effect, 100 volunteers with symptoms of hair thinning, hair line backward movement, hair loss and hair loss, dry hair and split ends and the like are selected for testing. After the hair restorer is used for 30 days and a proper amount of the hair restorer of the embodiment 1-4 is used every day for scalp massage and nursing, the scalp massage is carried out for 10-20 minutes until the hair restorer is absorbed by the scalp, the hair restorer can be repeatedly used for 1-3 times a day, and the effective rate reaches 70.23 percent through verification.

Seborrheic alopecia: the high temperature is caused by the exuberant sebum secretion, so the sebum secretion is more serious in summer and autumn than in winter and spring.

Comprehensive alopecia: comprises alopecia caused by endocrine dyscrasia, excessive stress, overstrain, and alopecia caused by lowering of body immune function or nerve dysfunction of scalp tissue after long-term decoction.

Chemical alopecia: hair cosmetics such as shampoo, permanent wave, hair dye, etc. contain a large amount of chemicals and may cause alopecia, and in addition, some chemicals such as daptomycin, allopurinol, etc. used for a long time may also cause drug-induced alopecia.

Nutritional alopecia: excessive sugar, excessive salt, protein deficiency, vitamin deficiency of B group, and trace elements deficiency all cause alopecia, and at this time, nutrition supplement is required to improve alopecia symptoms.

The results of the hair growth and loss prevention effect test of the fibroin hair-strengthening and loss prevention hair growth composition are shown in the following table 1.

Table 1 hair growth and loss prevention effect test of fibroin hair-strengthening and loss prevention hair growth-promoting compositions of examples 1 to 4

Explanation of hair growth and hair-securing effect:

① is very good, and has obvious hair growth and new hair growth on scalp hair follicle, ② effect is generally that hair loss is reduced, hair quality is tough, and some new hair growth occurs, ③ has no effect that there is no difference before and after use.

In addition, as shown in fig. 1a-1d, 15 volunteers are selected for use by a sample prepared by the verification formula of the invention, and from fig. 1c-1d, it can be seen that the degree of hair scale fragmentation is obviously reduced after the test effect diagram of the blank control combination added with the silk protein liquid is amplified by 1000 times and 4000 times, which indicates that the silk protein liquid has a certain repairing effect on hair scales, so that the invention has a certain effect on nutrition and hair fixation.

Based on the theory of promoting the activity of human fibroblasts and deeply repairing damaged scalp to improve the micro-ecological environment of scalp, the silk protein liquid is used for promoting the activity of skin fibroblasts, balancing immune barrier, improving the micro-ecological environment of scalp consisting of physiological lipid, natural moisturizing factors and scalp microbial population, maintaining the health of scalp and repairing damaged hair scales, thereby achieving the effects of growing hair and strengthening hair.

The results of multiple tests show that the hair growth liquid prepared by the formula of the functional raw materials without any auxiliary hair growth has the hair growth effect; meanwhile, the hair growing and strengthening effect can be more remarkable by matching with other functional components (traditional Chinese medicine extracting solution).

Effect test example 2

We then carried out a relevant test to certify the efficacy of our primary component fibroin solution, and we carried out an immune barrier study on the microecological reconstruction of the scalp and scalp with fibroin solution, with the results shown in tables 2 and 3 below.

Table 2 microecological reconstitution of fibroin solutions for sample groups 1-3 immune barrier test on scalp and scalp 1

Sample set	Relative content of 1L-1 a	1L-1 a percent reduction
			L PS +0.012mg/ml (sample group 1)	170.39	2.13
L PS +0.06mg/ml (sample set 2)	168.69	7.18
			L PS +0.3mg/ml (sample set 3)	156.01	9.02

Table 3 microecological reconstitution immune barrier test on scalp and scalp of fibroin solutions of sample groups 1-3 2

Sample set	Relative content of 1L-6	1L-6 percent reduction
			L PS +0.012mg/ml (sample group 1)	164.5	1.15
L PS +0.06mg/ml (sample set 2)	165.75	2.7
			L PS +0.3mg/ml (sample set 3)	156.39	10.45

The results in FIGS. 2-3 and tables 2-3 above show that the fibroin solutions of the examples of the present invention produced some inhibition of I L-1 a and I L-6 cytokine release from macrophages due to L PS at three concentrations tested, 0.012mg/m L, 0.06mg/m L, and 0.3mg/m L, with a tendency to increase with concentration.

In addition, Table 4 below shows that 0.30mg/m L and 1.2mg/m L promote the viability of human skin fibroblasts, and that 3.5mg/m L may produce some inhibition of the cells by the action of the hydrolysate.

TABLE 4 Effect of fibroin solutions on the viability of human skin fibroblasts

Sample concentration (mg/m L)	Viability(％)
		0	100.00±12.02
0.1	98.22±16.64
		0.3	120.68±35.86
1.2	132.42±35.28
		3.5	78.68±16.30

As shown in fig. 4, the fibroin solution and the control group were subjected to 2D cell repair test, which shows that cell migration started at 24h after the test, and that 48h clearly shows that the comparative NC blank group has significant effects on cell migration, proliferation and regeneration.

Effect test example 3

The application process and the principle of the anti-hair loss and hair-strengthening hair-growing liquid composition product containing the fibroin are as follows:

in order to more clearly illustrate the effects of the mutual matching and synergy of the hair-loss preventing, hair-strengthening and hair-restoring liquid composition of the present invention (tests have verified that the combination of the components of the present invention produces the technical effect of 1+1 greater than 2, as shown in the data of the following table 5), the present invention is supplemented with the following comparative examples and effect test examples on the basis of the primary hair-restoring liquid example 1 to illustrate:

table 5 component formulation of example 1 and comparative examples 1-5 (% by weight)

Note: s is the traditional Chinese medicine composition, and the weight is 0 percent; d-1 extract of arborvitae, fleece-flower root and Artemisia princeps, the weight ratio is 1:1:1, 0.3%; d-2 is the extract composition of cacumen Platycladi, Polygoni Multiflori radix, folium Artemisiae Argyi, semen Cuscutae, and fructus Ligustri Lucidi (ratio of 1:1:1:1:1), and weight is 0.5%; d-3 is the extract composition of ginseng, ganoderma atrum, liquorice, citrus peel and zanthoxylum fruit (the proportion is 1:1:1:1:1), and the weight is 0.5%; d-4 is the extract composition of cacumen Platycladi, Polygoni Multiflori radix, folium Artemisiae Argyi, semen Cuscutae, fructus Ligustri Lucidi, Ginseng radix, Ganoderma, Glycyrrhrizae radix, Mandarin orange pericarp, and fructus Zanthoxyli (ratio of 1:1:1:1: 1:1), with weight of 1.0%; d-5 is the extract composition of cacumen Platycladi, Polygoni Multiflori radix, folium Artemisiae Argyi, semen Cuscutae, fructus Ligustri Lucidi, Ginseng radix, Ganoderma, Glycyrrhrizae radix, Mandarin orange pericarp, and fructus Zanthoxyli (ratio of 1:1:1:1: 1:1), and weight is 2.0%;

the operation is as follows: in example 1, the phase a component is heated to 80 ℃ (± 1 ℃), stirred for 30min, cooled to 40 ℃, and added with B, C phase components, so as to obtain the hair growth promoting liquid product of the fibroin composition with the anti-hair loss and hair growth promoting effects.

The results of the tests for retarding hair loss and promoting hair growth, which were performed on example 1 and comparative examples 1 to 5, are shown in the following Table 6:

TABLE 6 results of hair loss prevention and hair growth promotion in example 1 and comparative examples 1 to 5

	Example 1	Comparative example 1	Comparative example 2	Comparative example 3	Comparative example 4	Comparative example 5
							The total effective rate%	63.3	70	73.3	63.3	83.3	83.3

As can be seen from Table 6, the anti-alopecia and hair-growing ability sequence is: example 1 ═ comparative example 3< comparative example 1 < comparative example 2 < comparative example 4 ═ comparative example 5.

The hair loss delaying and hair growth promoting agent is characterized in that no traditional Chinese medicine components are added in example 1, the silk protein liquid mainly contains α -bisabolol which has synergistic effects of nutrition supply to scalp hair follicles, activity promotion of fibroblasts and inflammatory factor inhibition, and cell regeneration promotion is achieved, so that hair loss is delayed and hair growth is promoted, the success rate of delaying hair loss and promoting hair growth of the traditional Chinese medicine composition in comparative example 3 is the same as that of example 1, and the synergistic effect of the traditional Chinese medicine composition and the silk protein liquid in comparative example 2 is higher than that of single example 1 and comparative example 2.

The difference between comparative example 4 and comparative example 5 is that one has fibroin solution, and the other has double success rate of the traditional Chinese medicine components without fibroin solution, but the success rate of the two in delaying hair loss or promoting hair is also higher than that of example 1 and comparative examples 1, 2 and 3, which shows that part of the components have the effect of providing nutrition for hair follicle cells, and part of the components have the effect of inhibiting 5 α -reductase, and the proliferation of hair outer root sheath cells is promoted.

Meanwhile, part of the components have the effect of stimulating hair follicle blasts, and other components have the effects of sterilizing and resisting inflammation, so that the hair loss is effectively prevented, and the hair growth is accelerated. In summary, the efficacy of each component in the present invention varies.

Meanwhile, experiments show that the reasonable matching of the components has important influence on the exertion of the efficacy, and the synergistic effect can be generated only by the reasonable matching of the components, so that the anti-hair loss and hair growth effects of the invention are achieved.

The specific combination and addition of the composition of the present invention within the scope of the claims can be found by comparing the experimental examples, and the composition has unique and obvious effects on preventing hair loss and promoting hair growth, which are different from the technical schemes disclosed in the prior art.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.

Claims

1. The anti-hair loss hair-strengthening hair-growing liquid composition containing fibroin is characterized by comprising the following raw material components in percentage by weight:

1.0-3.0% of butanediol, 0.01-0.1% of sodium hyaluronate, 0.01-0.1% of EDTA.2Na, 0.01-0.2% of allantoin, 0.1-1.0% of D-panthenol, 0.1-0.5% of α -bisabolol, 0.1-1.0% of hydrogenated castor oil, 0.03-1.0% of citric acid, 3.0-5.0% of silk protein liquid, 0-10% of traditional Chinese medicine extract, a proper amount of essence, a proper amount of preservative and the balance of deionized water.

2. The fibroin-containing hair loss prevention, hair solidification and hair growth promotion composition of claim 1, wherein: the content range of the traditional Chinese medicine extract is 0.1-10%; a preferred range is 1.0-5%.

3. The fibroin-containing hair loss prevention, hair solidification and hair growth promotion composition of claim 2, wherein: the traditional Chinese medicine extract is selected from one or more of 0.1-1.0% of Chinese arborvitae extract, 0.1-1.0% of polygonum multiflorum extract, 0.1-1.0% of south dodder seed extract, 0.1-1.0% of wormwood extract, 0.1-1.0% of privet extract, 0.1-1.0% of angelica sinensis extract, 0.1-1.0% of ginseng extract, 0.1-1.0% of ganoderma atrum extract, 0.1-1.0% of liquorice extract, 0.1-1.0% of citrus peel extract and 0.1-1.0% of zanthoxylum fruit extract.

4. The fibroin-containing hair loss prevention, hair solidification and hair growth promotion composition of claim 1, wherein: the fibroin solution is prepared by fresh cut cocoons by adopting the latest O-discharge double-circulation biochemical hydrolysis technology, controlling the hydrolysis degree of silk through hydrolytic enzyme, disorderly winding protein bundles of the silk, cutting the protein bundles into proteins with different grades and molecular weights, and combining the proteins to obtain the fibroin solution.

5. The fibroin-containing hair loss prevention, hair solidification and hair growth promotion composition of claim 4, wherein: by controlling the hydrolysis end point, the molecular weight of the fibroin of the obtained final product is controlled within the range of 2-20 ten thousand daltons, and the molecular weight is mainly centralized within the range of 10-20 ten thousand daltons.

6. The fibroin-containing hair loss prevention, solidification and growth promotion liquid composition as set forth in claim 2 is characterized by comprising the following raw material components in parts by weight:

94.3 percent of deionized water; 1.0% of butanediol; 0.05% of sodium hyaluronate; 0.05 percent of EDTA.2Na; 0.05% of allantoin; 0.1% of D-panthenol;

α -bisabolol 0.1%, hydrogenated castor oil 0.2%;

7. The fibroin-containing hair loss prevention, solidification and growth promotion liquid composition as set forth in claim 2 is characterized by comprising the following raw material components in parts by weight:

80.2% of deionized water; 3.0% of butanediol; 0.1% of EDTA.2Na; 0.05% of sodium hyaluronate; 0.05% of allantoin; 0.5 percent of D-panthenol;

α -bisabolol 0.5%, hydrogenated castor oil 0.5%;

4.0% of silk protein liquid; 1.0% of arborvitae extract; 1.0% of polygonum multiflorum extract; south dodder seed extract 1.0%; 1.0% of artemisia scoparia extract; 1.0% of privet extract; 1.0% of angelica sinensis extract; 1.0% of ginseng extract; 1.0% of ganoderma atrum extract; 1.0% of liquorice extract; 1.0% of citrus peel extract; 1.0% of zanthoxylum bungeanum fruit extract; 0.03 percent of citric acid; a proper amount of essence; proper amount of preservative.

8. The method for preparing the fibroin-containing anti-hair loss, hair-fixing and hair-growing liquid composition as claimed in any one of claims 1 to 7, which comprises the following steps:

2) after the mixture A in the step 1) is completely dissolved, α -bisabolol and hydrogenated castor oil are mixed, stirred and dissolved uniformly to prepare a mixture B at the temperature of about 60-70 ℃;

3) after the temperature is reduced to about 50-60 ℃, proper amounts of traditional Chinese medicine extract, silk protein liquid, citric acid, essence and preservative are sequentially added into the mixture body B in the step 2), and the mixture body B is uniformly stirred and mixed, so that the hair-loss preventing, hair-consolidating and hair-growing liquid composition can be obtained.

9. The method for preparing the fibroin-containing hair loss prevention, hair solidification and hair growth promotion composition according to claim 8, comprising the steps of:

the component A comprises the following components: 94.3 percent of deionized water; 1.0% of butanediol; 0.05% of sodium hyaluronate; 0.01 percent of EDTA.2Na; 0.01 percent of allantoin; 0.1% of D-panthenol;

the component B comprises α -bisabolol 0.1%, and hydrogenated castor oil 0.2%;

c phase component: 3.0% of silk protein liquid; 0.1% of arborvitae extract; 0.1% of polygonum multiflorum extract; 0.1% of south dodder seed extract; 0.1% of Artemisia princeps Pampanini extract; 0.1% of privet extract; 0.1% of angelica extract; 0.1% of ginseng extract; 0.1% of ganoderma atrum extract; 0.1% of licorice extract; citrus peel extract 0.1%; 0.1% of zanthoxylum bungeanum fruit extract; 0.03 percent of citric acid; a proper amount of essence; a proper amount of preservative;