CN111374902A

CN111374902A - Anti-hair loss and hair-fixing shampoo composition containing fibroin and preparation method thereof

Info

Publication number: CN111374902A
Application number: CN202010260226.0A
Authority: CN
Inventors: 张向阳; 甘景苗
Original assignee: Guangzhou Qiyuan Biotechnology Co ltd
Current assignee: Guangzhou Qiyuan Biotechnology Co ltd
Priority date: 2020-04-03
Filing date: 2020-04-03
Publication date: 2020-07-07

Abstract

The invention discloses a hair-strengthening and hair-loss-preventing shampoo composition containing fibroin and a preparation method thereof. The shampoo composition comprises the following raw material components: 54.0 to 67.0 percent of deionized water; 14.0 to 18.0 percent of sodium laureth sulfate; ethylene glycol distearate 1.0-2.0%; 1.0-3.0% of cocamide methyl MEA; 0.1-0.5% of soapberry extract; 5.0 to 10.0 percent of water; 0.1 to 0.3 percent of guar hydroxypropyl trimethyl ammonium chloride; EDTA-2Na 0.01-0.5%; 0.01-0.1% of allantoin; polyquaternium-470.3-2.0%; 1.0-3.0% of polydimethylsiloxane/lauryl ether polyether-23/sodium lauryl polyether sulfate; 1.0-3.0% of dimethiconol/dodecyl benzene sulfonic acid triethanolamine salt; cocamidopropyl betaine 2.0-5.0%; silk protein liquid 3.0-5.0%; ginger extract 0.1-1.0%; 0-10% of traditional Chinese medicine extract; tea tree oil 0.1-1.0%; 0.03-1.0% of citric acid; proper amount of preservative. The invention rebuilds and protects the scalp and scalp microecology by silk protein liquid, ginger extract, soapberry extract and the like, thereby achieving the purposes of growing hair and fixing hair and simultaneously enhancing the toughness of hair.

Description

Anti-hair loss and hair-fixing shampoo composition containing fibroin and preparation method thereof

Technical Field

The invention relates to a hair-strengthening and hair-loss-preventing shampoo composition, belongs to the technical field of hair-growing and hair-nourishing products, and particularly relates to a fibroin-containing hair-strengthening and hair-loss-preventing shampoo composition and a preparation method thereof.

Background

In the current social development, there are many influences of internal factors such as mental stress caused by deterioration of air quality, increase in living pressure, insufficient sleep in night life, and the like, and hormonal disorders in the body. More and more consumers are beginning to develop scalp problems such as scalp inflammation, hair oil, excessive hair loss and alopecia areata.

At present, most of hair loss prevention and hair growth promotion shampoo products on the market are pure traditional Chinese medicine preparations which are matched with more than ten or dozens of traditional Chinese medicine components, the components are complex, and the real hair loss prevention effect is not obvious.

In addition, hair growth promoting products containing western medicine components can also cause irritation (red swelling, dandruff and burning pain) and contact anaphylaxis of skin. Some patients may develop various adverse symptoms such as itching, wheal, allergic rhinitis, swelling and redness of the face, and desquamation of the scalp. Individual patients also develop physical symptoms such as chest distress, shortness of breath, headache, neuritis, dizziness, syncope, vertigo, edema, chest pain, blood pressure change, palpitation, pulse frequency change and the like. The user in this part has to stop taking the medicine and the good treatment and use effect can not be obtained.

Therefore, the existing anti-hair loss and hair growth products still need to be further improved in formula and process.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide a hair-strengthening and hair-loss-preventing shampoo composition containing fibroin, which is mainly used for rebuilding and protecting the scalp and the scalp microecology by compounding fibroin liquid with other components, so that the aims of growing and strengthening the hair are fulfilled, meanwhile, the toughness of the hair can be enhanced, and the technical effect of hair loss prevention is really achieved.

The invention also aims to provide a preparation method of the hair-strengthening and hair-loss-preventing shampoo composition containing the fibroin.

The purpose of the invention and the technical problem to be solved are realized by adopting the following technical scheme. The invention provides a fibroin-containing hair loss prevention and hair solidification shampoo composition which comprises the following raw material components in percentage by weight:

14.0 to 18.0 percent of sodium laureth sulfate; ethylene glycol distearate 1.0-2.0%; 1.0-3.0% of cocamide methyl MEA; 0.1-0.5% of soapberry extract;

0.1 to 0.3 percent of guar hydroxypropyl trimethyl ammonium chloride; EDTA-2Na 0.01-0.5%; 0.01-0.1% of allantoin; polyquaternium-470.3-2.0%; 1.0-3.0% of polydimethylsiloxane/lauryl ether polyether-23/sodium lauryl polyether sulfate; 1.0-3.0% of dimethiconol/dodecyl benzene sulfonic acid triethanolamine salt; cocamidopropyl betaine 2.0-5.0%;

silk protein liquid 3.0-5.0%; ginger extract 0.10-1.0%; tea tree oil 0.10-1.0%; 0-10% of traditional Chinese medicine extract; 0.03-1.0% of citric acid; proper amount of preservative and the balance of deionized water.

Further, the content range of the traditional Chinese medicine extract in the anti-hair loss and hair-strengthening shampoo composition containing fibroin is 0-10%; the preferred range is 1-5%;

the traditional Chinese medicine extract is selected from one or more of 0.1-1.0% of Chinese arborvitae extract, 0.1-1.0% of polygonum multiflorum extract, 0.1-1.0% of south dodder seed extract, 0.1-1.0% of artemisia vulgaris extract, 0.1-1.0% of privet extract, 0.1-1.0% of sophora flavescens extract, 0.1-1.0% of ligusticum wallichii extract, 0.1-1.0% of rhizoma kaempferiae root extract, 0.1-1.0% of liquorice extract, 0.1-1.0% of citrus peel extract and 0.1-1.0% of zanthoxylum fruit extract.

Further, in the anti-hair loss and hair-strengthening shampoo composition containing silk protein, the silk protein liquid is prepared by fresh cut cocoons by adopting the latest O-discharge double-circulation biochemical hydrolysis technology, controlling the hydrolysis degree of silk by using hydrolytic enzyme, disorderly winding protein bundles of the silk, cutting the protein bundles into proteins with different grades and molecular weights, and combining the proteins to obtain the silk protein liquid;

the specific preparation process of the silk protein liquid comprises the following steps:

1) 0.4 to 6 mass percent of Na is used₂CO₂Or NaHCO₃The aqueous solution is used as degumming agent for degumming silkworm cocoons under the conditions that the pH value is 8-11, the hydrolysis temperature is 50-80 ℃ and the time is 2-4 hours; filtering after the degumming is finished to obtain degumming hydrolysate and silk fibroin fibers;

2) removing salt in the degumming hydrolysate through electrodialysis, obtaining sericin hydrolysate and dialysis ion solution in the process, retaining the sericin hydrolysate, and transferring the dialysis ion solution to next preparation of the sericin hydrolysate for use;

3) dissolving the silk fibroin fibers obtained by filtering in the step 1) in CaCl with the mass percent of 30-60% at the temperature of 60-100 DEG C₂、CaCl₂-H₂O、CaCl₂-2H₂Hydrolyzing in neutral salt solution of O or LiBr to obtain silk fibroin solution;

4) performing electrodialysis on the hydrolyzed silk fibroin solution obtained in the step 3) to remove ions in the solution, and circulating the solution until the conductivity of the solution reaches 10-500us/cm to obtain a light yellow silk fibroin pure solution, wherein the ion solution after electrodialysis can be circularly used as a neutral salt solution in the step 4);

5) and (3) uniformly mixing the sericin hydrolysate obtained in the step (2) and the fibroin pure solution obtained in the step (4) to obtain the fibroin solution.

Further, in the above anti-hair loss and hair-fixing shampoo composition containing fibroin, in step 1), the method for cultivating the cut cocoons of fibroin liquid comprises the following steps:

1.1) selecting mulberry field:

mulberry trees are planted in the Yunnan mountain area with sufficient sunlight and large day-night temperature difference, and the mulberry leaves have high sugar content and are beneficial to the healthy growth of the silkworm, so that high-quality cocoon shells with thick shells and large sizes are obtained; in addition, a natural barrier is formed by the unique small basin structure in the mountainous area of Yunnan, so that the pesticide in the non-silkworm rearing area is reduced from scattering to the silkworm rearing area, and the pesticide residue of the final product is guaranteed;

1.2) selection of silkworm species:

the breeding silkworm is a second generation silkworm obtained from a silkworm industry technical station, and the breeding silkworm is adopted for the purpose of stable content of the obtained sericin protein and the silk fibroin protein, so that a hydrolysis final product with a stable ratio of the hydrolyzed sericin protein to the hydrolyzed silk fibroin protein is obtained;

1.3) culture environment:

the culture environment of the silkworm is related to the microbial control of the later-stage product and silkworm leaf disease; the silkworm breeding environment adopts aseptic, ventilated and dry silkworm breeding in a room so as to reduce the risks of germs, microorganisms and the like of a final product;

1.4) storage conditions:

silk is an active protein and needs to be stored in a cool, dry, ventilated place.

Further, in the above anti-hair loss and hair-fixing shampoo composition containing fibroin, in the step 5), the molecular weight of the fibroin of the final product obtained in the step 5) is controlled within an interval of 2-20 ten thousand daltons by controlling the hydrolysis end point, and is mainly concentrated within an interval of 10-20 ten thousand daltons.

Further, the anti-hair loss and hair-fixing shampoo composition containing the fibroin comprises the following raw material components in parts by weight:

73.0% of deionized water; 16% of sodium laureth sulfate; ethylene glycol distearate 1.0%; cocoamide methyl MEA 1.0%; 0.1% of soapberry extract;

0.1% of guar hydroxypropyl trimethyl ammonium chloride; EDTA-2Na 0.05%; 0.05% of allantoin; polyquaternium-470.3%; 1.0 percent of polydimethylsiloxane/lauryl ether polyether-23/sodium lauryl polyether sulfate; 1.0 percent of dimethiconol/dodecyl benzene sulfonic acid triethanolamine salt; cocamidopropyl betaine 2.0%;

3.0% of silk protein liquid; ginger extract 0.1%; tea tree oil 0.10%; 0.03 percent of citric acid; proper amount of preservative.

56.3 percent of deionized water; sodium laureth sulfate 18%; ethylene glycol distearate 1.0%; 2.0% of cocamide methyl MEA; 0.2% of soapberry extract;

0.2% of guar hydroxypropyl trimethyl ammonium chloride; EDTA-2Na 0.05%; 0.1 percent of allantoin; polyquaternium-471.0%; 2.0 percent of polydimethylsiloxane/lauryl ether polyether-23/sodium lauryl polyether sulfate; 1.0 percent of dimethiconol/dodecyl benzene sulfonic acid triethanolamine salt; cocamidopropyl betaine 2.0%;

4.0% of silk protein liquid; 1.0% of arborvitae extract; 1.0% of polygonum multiflorum extract; south dodder seed extract 1.0%; 1.0% of artemisia scoparia extract; 1.0% of privet extract; 1.0% of sophora flavescens extract; 1.0% of ligusticum wallichii extract; kaempferia root extract 1.0%; 1.0% of liquorice extract; 1.0% of citrus peel extract; 1.0% of zanthoxylum bungeanum fruit extract; 1.0% of ginger extract; tea tree oil 0.10%; 0.03 percent of citric acid; proper amount of preservative.

In addition, the invention also discloses a preparation method of the anti-hair loss and hair-fixing shampoo composition containing fibroin, which comprises the following steps:

1) firstly, stirring, mixing and dissolving deionized water, sodium laureth sulfate, ethylene glycol distearate, cocoamidomethyl MEA (methyl MEA) and guar gum hydroxypropyl trimethyl ammonium chloride completely at the temperature of about 80-85 ℃ to avoid excessive foam as much as possible, and vacuumizing and stirring; keeping the temperature of the mixture body A for half an hour;

2) after the mixed material A in the step 1) is completely dissolved, sequentially adding EDTA-2Na, allantoin, polyquaternium-47, polydimethylsiloxane/laureth-23/laureth sodium sulfate, dimethiconol/laureth triethanolamine salt and cocamidopropyl betaine, stirring and completely dissolving to prepare a mixed material B at the temperature of about 60-70 ℃;

3) and (3) after the temperature is reduced to about 50-60 ℃, sequentially adding a proper amount of traditional Chinese medicine extract, silk protein liquid, ginger extract, tea tree oil, citric acid and preservative into the mixture body B in the step 2), uniformly stirring and mixing, and discharging to prepare the anti-hair loss and hair-consolidating shampoo composition.

Further, the preparation method of the anti-hair loss and hair-fixing shampoo composition containing fibroin comprises the following steps:

the anti-hair loss and hair-fixing shampoo composition containing the fibroin is prepared from the following components in percentage by weight:

the component A comprises the following components: 66.2% of deionized water; sodium laureth sulfate 18%; ethylene glycol distearate 1.0%; 2.0% of cocamide methyl MEA; 0.2% of guar hydroxypropyl trimethyl ammonium chloride;

b component: EDTA-2 Na0.05%; 0.1 percent of allantoin; polyquaternium-471.0%; 2.0 percent of polydimethylsiloxane/lauryl ether polyether-23/sodium lauryl polyether sulfate; 1.0 percent of dimethiconol/dodecyl benzene sulfonic acid triethanolamine salt; cocamidopropyl betaine 2.0%;

c phase component: 0.2% of soapberry extract; 4.0% of silk protein liquid; 1.0% of ginger extract; 0.1% of arborvitae extract; 0.1% of polygonum multiflorum extract; 0.1% of south dodder seed extract; 0.1% of Artemisia princeps Pampanini extract; 0.1% of privet extract; 0.1% of sophora flavescens extract; 0.1% of ligusticum wallichii extract; rhizoma Kaempferiae extract 0.1%; 0.1% of licorice extract; citrus peel extract 0.1%; 0.1% of zanthoxylum bungeanum fruit extract; tea tree oil 0.10%; 0.03 percent of citric acid; a proper amount of preservative;

the preparation method of the anti-hair loss and hair-fixing shampoo composition containing the fibroin comprises the following steps;

1) firstly, stirring, mixing and dissolving the phase A completely at the temperature of about 80-85 ℃, avoiding excessive foam generation as much as possible, and vacuumizing and stirring; preserving the heat for half an hour;

2) adding B after the A phase is completely dissolved, stirring and dissolving completely at 60-70 deg.C

3) And after the temperature is reduced to about 50-60 ℃, adding the phase C into the material body, stirring and mixing uniformly, and discharging.

The source and the effect of the raw material components of the invention are explained as follows:

① Silk protein liquid

The fibroin liquid is a small and medium molecular fibroin hydrolyzed mixed liquid obtained by using fresh cut cocoons and adopting a 0-discharge twice circulating hydrolysis process. The invention adopts the latest biochemical technology, controls the hydrolysis degree of silk by hydrolase, disorderly twines the protein bundles of the silk, cuts the protein bundles into proteins with different grades and molecular weights, and combines the proteins to obtain the silk protein liquid. The protein molecule has surface activity, can increase the foam stability of a system, prolong the foam retention time of a compound system, and simultaneously can reduce the irritation of other surfactants. The fibroin liquid contains keratin, small molecular peptides, amino acids and other parts which can be absorbed and utilized by scalp, effectively repairs damaged hair scales, and can inhibit the release of inflammatory substances, so the fibroin liquid has a certain itching relieving effect, and simultaneously keratin can promote the activity of human skin fibroblasts and is beneficial to hair growth and hair strengthening.

② rhizoma Zingiberis recens extractive solution

The ginger extract is an effective component extracted from high-quality ginger, contains volatile oil, gingerol, zingerone and a plurality of amino acids, has the effects of stimulating skin, promoting blood circulation and the like, and can prevent and treat dandruff and promote hair growth. When applied externally to the scalp, ginger can engorge the scalp and promote blood circulation, and blood is one of the key factors for hair growth. Therefore, the blood circulation stimulating effect of the congestion can improve the growth of hair to a certain extent, and the effect is very obvious especially for people suffering from psychogenic alopecia and kidney deficiency alopecia.

③ phase A component

The sodium laureth sulfate in the phase A component is mainly used as a main surfactant and mainly plays a role in cleaning, decontaminating and foaming. The cocamide methyl MEA is a nonionic surfactant and has freeze resistance, thickening, foaming and foam stabilizing properties. The glycol distearate is used to impart pearly or milky appearance to the product, and the guar hydroxypropyl trimonium chloride can regulate the consistency of the system, has antistatic effect, and can improve wet combing property and dry combing property of hair, and make the hair retain luster, softness and elasticity for a long period of time.

④ B phase component

In the component B, EDTA-2Na is a metal chelating agent and can chelate metal ions in the product; allantoin has the effects of promoting the generation of skin cells, rapidly healing wounds, being a good healing agent for skin wounds, softening keratin, keeping the skin moist, moistening and softening; the polyquaternium-47 is used as a conditioner in the formula, so that the hair has the advantages of electric resistance, softness, moistening and luster; the polydimethylsiloxane/lauryl ether polyether-23/sodium lauryl polyether sulfate is emulsified silicone oil with large particle size, and has the effects of improving dry and wet combing property, protecting hair, avoiding damage, increasing luster and softening hair; the dimethiconol/dodecylbenzene sulfonic acid triethanolamine salt is emulsified silicone oil with small particle size, can improve wet combing property and dry combing property of hair, and enables the hair to maintain luster, softness and elasticity for a long time; the cocamidopropyl betaine is an anionic surfactant and mainly plays roles in thickening, cleaning and resisting static in the formula;

⑤ C phase component:

in the formula, the soapberry extract in the C phase component mainly plays the roles of sterilizing, relieving itching, removing dirt and foaming; the fibroin solution rebuilds and protects the scalp and scalp microecology, thereby achieving the purposes of growing and fixing hair; meanwhile, the toughness of the hair can be enhanced; the tea tree oil mainly plays a role in controlling oil and relieving itching in the formula; adjusting the pH value with citric acid; the preservative keeps breeding of microorganisms, and the service life of the product is prolonged.

⑥ Chinese medicinal extract

The traditional Chinese medicine extract in the embodiment of the invention adopts traditional Chinese medicine extracting solution which is an optional component, wherein the arborvitae extract mainly has the effects of growing and blackening hair and improving the production environment of scalp so as to prevent and cure hair, the polygonum multiflorum extracting solution mainly has the effect of benefiting essence and blood and can play a role of blackening and brightening hair in shampoo, the south dodder extract mainly has an inhibiting effect on 5 α -reductase, the artemisia vulgaris extract can promote hair growth, the privet extract can promote melanin synthesis, the sophora flavescens extract has the effects of sterilization and inflammation diminishing, the ligusticum wallichii extract can expand head capillaries and promote blood circulation to increase the nutrition supply of hair, the kaempferia galanga root extract has an antioxidation effect, and the extracts of liquorice extract, citrus peel extract, zanthoxylum bungeanum fruit and the like can nourish hair roots, so that the effect of activating hair follicle mother cells can be achieved, alopecia can be effectively prevented, and hair growth can be accelerated.

By means of the technical scheme, the invention has the following advantages and beneficial technical effects:

1) in the silk protein liquid component in the hair loss prevention and hair-fixing shampoo disclosed by the invention, the small molecular peptides and amino acids can be partially absorbed and utilized by the scalp, and have nourishing and nourishing effects on the scalp and hair; macromolecular sericin and keratin can form a 'mud' cell adhesion substance on the surfaces of the scalp and the hair, and the 'mud' cell adhesion substance can repair epidermal cells to be peeled off and delay the shedding of the scalp; also can repair hair stem, reduce hair brittle fracture, bifurcation and shedding, and has the efficacy after being continuously used for one month;

2) in the shampoo composition, the silk protein liquid is used together with other components (traditional Chinese medicine extract, ginger extract and tea tree oil), so that the toughness of hair can be enhanced; the silk hydrolysate is used in combination with other components (soapberry extract, etc.) to rebuild and protect scalp and scalp microecology, thereby achieving the purpose of hair growth and hair-fixing.

3) The invention rebuilds and protects the scalp and scalp microecology by silk protein liquid, ginger extract, soapberry extract and the like, thereby achieving the purposes of hair growth and hair fixation; meanwhile, the toughness of the hair can be enhanced.

Drawings

Fig. 1(a) is a diagram showing scalp hair before using the hair loss preventing and hair fixing shampoo composition containing fibroin according to the present invention;

fig. 1(b) is a diagram showing scalp hair after using the hair loss prevention and hair solidification shampoo composition containing fibroin according to the present invention;

FIG. 2 is a graph showing the percentage statistics of 1L-1a and 1L-6 macrophage release by LPS for different fibroin fluid concentrations according to the present invention;

FIG. 3 is a histogram of the human skin fibroblast viability for different fibroin fluid concentrations of the present invention.

Detailed Description

The shampoo composition disclosed by the invention contains a silk protein liquid component, mainly consists of small and medium-sized protein molecules with the molecular weight of 2-20 ten thousand Da, is rich in silk protein, peptide, amino acid and the like, and has the functions of nourishing and repairing hair, scalp and hair roots.

The invention discloses a hair-strengthening and hair-loss-preventing shampoo composition containing fibroin and a preparation method thereof. The shampoo composition comprises the following raw material components:

deionized water; sodium laureth sulfate; ethylene glycol distearate; cocoamide methyl MEA; soapberry extract; water; guar hydroxypropyltrimonium chloride; EDTA-2 Na; allantoin; polyquaternium-47; polydimethylsiloxane/laureth-23/sodium laureth sulfate; dimethiconol/triethanolamine dodecylbenzene sulfonate; cocamidopropyl betaine; silk protein liquid; a ginger extract; tea tree oil 0.1-1.0%; citric acid; proper amount of preservative.

The invention rebuilds and protects the scalp and scalp microecology by silk protein liquid, ginger extract, soapberry extract and the like, thereby achieving the purposes of hair growth and hair fixation; meanwhile, the toughness of the hair can be enhanced.

The specific process of the silk protein liquid is as follows:

the fibroin solution is prepared by fresh cut cocoons by adopting the latest O-discharge double-circulation biochemical hydrolysis technology, controlling the hydrolysis degree of silk through hydrolytic enzyme, disorderly winding protein bundles of the silk, cutting the protein bundles into proteins with different grades and molecular weights, and combining the proteins to obtain the fibroin solution. The method specifically comprises the following process steps:

2) removing salt in the degumming hydrolysate through electrodialysis, obtaining sericin hydrolysate and dialysis ion solution from the degumming hydrolysate, retaining the sericin hydrolysate, and transferring the dialysis ion solution to next preparation of the sericin hydrolysate for use;

5) uniformly mixing the sericin hydrolysate obtained in the step 2) and the fibroin pure solution obtained in the step 4) to obtain fibroin liquid; by controlling the hydrolysis end point, the molecular weight of the fibroin of the obtained final product is controlled within the range of 2-20 ten thousand daltons, and the molecular weight is mainly centralized within the range of 10-20 ten thousand daltons.

In the silk hydrolysate disclosed by the invention, small molecular peptides and amino acids can be partially absorbed and utilized by the scalp, and have nourishing and nourishing effects on the scalp and hair. The macromolecular sericin and keratin can form a cell adhesion substance like 'mud' on the surfaces of the scalp and the hair, and the cell adhesion substance can repair epidermal cells to be peeled off and delay the shedding of the scalp. Also can repair hair stem, reduce hair brittle fracture, bifurcation and shedding, and has the effect after being continuously used for one month. Meanwhile, the toughness of the hair can be enhanced. Therefore, the invention mainly adopts the silk hydrolysate to rebuild and protect the scalp and scalp microecology, thereby achieving the purposes of hair growth and hair-fixing.

The present invention is further defined by the following specific preferred embodiments, but the present invention is not limited to the following embodiments. The invention mainly adopts fibroin liquid, is added into a hair washing and protecting formula to prepare a product containing fibroin with the functions of fixing hair, preventing hair loss and growing hair, and describes the process operation of the product formula.

Example 1

A hair-strengthening and hair-loss-preventing shampoo composition containing fibroin is prepared from the following raw material components in percentage by weight:

phase A component:

74.82% of water; sodium laureth sulfate 18.0%; ethylene glycol distearate 1.0%; cocoamide methyl MEA 1.8%; 0.2% of guar hydroxypropyl trimethyl ammonium chloride;

b phase component:

EDTA-2Na 0.05%; 0.1 percent of allantoin; polyquaternium-471.0%; 2.0 percent of polydimethylsiloxane/lauryl ether polyether-23/sodium lauryl polyether sulfate; 1.0 percent of dimethiconol/dodecyl benzene sulfonic acid triethanolamine salt; 2.0% of cocamidopropyl betaine;

c phase component:

4.0% of silk protein liquid; 0.2% of soapberry extract; 2.0% of ginger extract; tea tree oil 0.2%; 0.03 percent of citric acid; 0.1% of methylisothiazolinone; 0.5 percent of essence.

Example 2

phase A component:

67.92% of water; 16.0 percent of sodium laureth sulfate; ethylene glycol distearate 1.0%; cocoamide methyl MEA 1.8%; 0.2% of guar hydroxypropyl trimethyl ammonium chloride;

b phase component:

EDTA-2Na 0.05%; 0.1 percent of allantoin; polyquaternium-470.5%; 2.5 percent of polydimethylsiloxane/lauryl ether polyether-23/sodium lauryl polyether sulfate; 1.0 percent of dimethiconol/dodecyl benzene sulfonic acid triethanolamine salt; cocamidopropyl betaine 3.0%;

c phase component:

3.0% of silk protein liquid; 0.2% of soapberry extract; 2.0% of ginger extract; tea tree oil 0.2%; 0.03 percent of citric acid; 0.1% of methylisothiazolinone; 0.5 percent of essence;

the anti-hair loss and hair-fixing shampoo composition containing fibroin of the above example 1-2 is prepared by the following process steps:

1) stirring, mixing and dissolving the phase A completely at the temperature of about 80-85 ℃, keeping the temperature and stirring for about half an hour, avoiding excessive foam generation as much as possible, and performing vacuum-pumping stirring;

2) adding the material B after the phase A is completely dissolved, stirring and completely dissolving, and keeping the temperature at 50-60 ℃; preserving the heat for half an hour;

3) and after the temperature is reduced to about 40-50 ℃, sequentially adding the phase C into the material body, and uniformly stirring and mixing to obtain the material.

Example 3

phase A component:

65.72% of water; 16.0 percent of sodium laureth sulfate; ethylene glycol distearate 1.0%; cocoamide methyl MEA 1.8%; 0.2% of guar hydroxypropyl trimethyl ammonium chloride;

b phase component:

c phase component:

3.0% of silk protein liquid; 0.2% of soapberry extract; 2.0% of ginger extract; cacumen Platycladi extract 0.5%; 0.5% of polygonum multiflorum extract; 0.5% of south dodder seed extract; 0.5% of Artemisia princeps Pampanini extract; glossy privet fruit extract 0.5%; 0.1% of sophora flavescens root extract; rhizoma Ligustici Chuanxiong root extract 0.1%; rhizoma Kaempferiae extract 0.1%; licorice root extract 0.1%; citrus peel extract 0.1%; 0.1% of zanthoxylum bungeanum fruit extract; tea tree oil 0.2%; 0.03 percent of citric acid; 0.1% of methylisothiazolinone; 0.5 percent of essence.

Example 4

phase A component:

63.51 percent of water; sodium laureth sulfate 18.0%; ethylene glycol distearate 1.0%; cocoamide methyl MEA 1.8%; 0.2% of guar hydroxypropyl trimethyl ammonium chloride;

b phase component:

c phase component:

4.0% of silk protein liquid; 0.2% of soapberry extract; 2.0% of ginger extract; cacumen Platycladi extract 0.2%; 0.2% of polygonum multiflorum extract; 0.2% of south dodder seed extract; 0.2% of artemisia scoparia extract; glossy privet fruit extract 0.2%; 0.2 percent of sophora flavescens root extract; rhizoma Ligustici Chuanxiong root extract 0.2%; rhizoma Kaempferiae extract 0.2%; licorice root extract 0.2%; citrus peel extract 0.2%; 0.2% of zanthoxylum bungeanum fruit extract; tea tree oil 0.3%; 0.04% of citric acid; 0.1% of methylisothiazolinone; 0.5 percent of essence.

The anti-hair loss and hair-fixing shampoo composition containing fibroin of the above examples 3-4 is prepared by the following process steps:

1) the phase A component is stirred, mixed and dissolved completely at the temperature of about 80-85 ℃, stirred for about half an hour under the condition of heat preservation, excessive foam is avoided as much as possible, and vacuumizing stirring can be carried out.

2) And adding the phase B component after the phase A component is completely dissolved, stirring and completely dissolving, and keeping the temperature at 50-60 ℃. The temperature was maintained for half an hour.

3) And after the temperature is reduced to about 40-50 ℃, sequentially adding the C-phase components into the material body, and uniformly stirring and mixing to obtain the material.

The following are contents of the effect test examples.

Effect experiment example 1

The application process and the principle of the anti-hair loss and hair-fixing shampoo composition product containing the fibroin are as follows:

firstly, when the product is used, the shampoo needs to be washed twice for one time, the dosage of the shampoo for the first time can be a little less, and the shampoo is primarily cleaned; the second dose may be slightly more than necessary for intensive care. When the product is used, the hair is wetted, the shampoo is poured into the palm, then the recommended dosage is about 5-10ml, and then the hair is kneaded, the scalp is massaged, and the shampoo is washed clean after a few minutes.

Besides being used as a basic formula of shampoo, the product of the invention is mainly added with fibroin liquid. The silk protein liquid is rich in silk protein, peptide, amino acid and the like, has the functions of nourishing and repairing hair, scalp and hair root and can reconstruct and protect the scalp and scalp microecology, thereby achieving the purposes of growing hair and fixing hair; meanwhile, the toughness of the hair can be enhanced. Certainly, a plurality of traditional Chinese medicine components are added in the formula for synergistic interaction, and the traditional Chinese medicine components can deeply nourish hair roots, can achieve the effect of activating hair follicle blasts, effectively prevent alopecia and can accelerate hair growth.

As shown in fig. 1(a) and 1(b), in the above formula, it can be seen that the main functional raw materials for promoting hair growth, strengthening hair and preventing hair loss are only 12 kinds of Chinese medicinal extract raw materials such as the silk protein liquid component of the present invention and ginger extract, and the total addition amount of the Chinese medicinal raw materials such as the ginger extract is only about 3%, and the addition amount of the silk protein liquid component of the present invention is 4.0%. The aims of hair growth, hair-fixing and hair loss prevention can be achieved.

The use effect of the product can be seen in fig. 1(a) and 1 (b); the graph is a comparison graph of the effect of the shampoo product before and after use, and the hair amount on the top of the head and on both sides of the forehead is obviously increased after the shampoo product is used for 22 days.

Effect test example 2

Then, we have also made relevant tests for the efficacy certification of our main ingredient fibroin solution, and we have made studies on the immune barrier in terms of the microecological reconstruction of the scalp and scalp by fibroin solution, and the results are shown in tables 1 and 2 below.

Table 1 microecological reconstitution of fibroin solutions for sample groups 1-3 immune barrier test on scalp and scalp 1

Sample set	Relative content of 1L-1a	Percent reduction of 1L-1a
			LPS +0.012mg/ml (sample group 1)	182.74	1.15
LPS +0.06mg/ml (sample group 2)	175.56	5.04
			LPS +0.3mg/ml (sample group 3)	166.54	9.92

Table 2 microecological reconstitution immune barrier test on scalp and scalp of fibroin solutions of sample groups 1-3 2

Sample set	Relative content of 1L-6	1L-6 percent reduction
			LPS +0.012mg/ml (sample group 1)	190.81	0.60
LPS +0.06mg/ml (sample group 2)	188.11	2.01
			LPS +0.3mg/ml (sample group 3)	177.66	7.45

The results of FIGS. 2-3 and tables 1-2 above show that: the fibroin solutions of the examples of the present invention produced some inhibition of IL-1a and IL-6 cytokine release from macrophages induced by LPS at three concentrations tested, 0.012mg/mL, 0.06mg/mL, 0.3mg/mL, and tended to increase with concentration.

In addition, Table 3 below shows that 0.32mg/mL and 1mg/mL promote the viability of human skin fibroblasts, and that 3.16mg/mL may produce some inhibition of the cells due to the action of the hydrolysate.

TABLE 3 Effect of fibroin solutions on the viability of human skin fibroblasts

Sample concentration (mg/mL)	Viability(％)
		0	100.00±14.42
0.1	98.22±18.44
		0.32	124.57±41.29
1	127.79±33.48
		3.16	74.56±18.50

The data in Table 3 above demonstrate that our fibroin solutions are capable of supporting cell growth, and have antibacterial, anti-inflammatory, immune barrier balancing, and deep scalp repair effects.

Effect test example 3

In order to more clearly illustrate the effects of the mutual matching and synergy of the fibroin solution, the ginger extract and the soapberry extract (tests prove that the technical effect of 1+1+1 greater than 3 is generated by the compound use of the components of the invention, as shown in the data in the following table 4), the invention is supplemented with the following comparative examples and effect test examples on the basis of the original shampoo example 1 for illustration:

table 4 component formulations of example 1 and comparative examples 1-5 (% by weight)

Note: s is the traditional Chinese medicine composition, and the weight is 0 percent; d-1 is the extract composition of cacumen Platycladi, Polygoni Multiflori radix, folium Artemisiae Argyi, semen Cuscutae, and fructus Ligustri Lucidi (ratio of 1:1:1:1:1), and weight is 0.5%; d-2 is the extract composition of cacumen Platycladi, Polygoni Multiflori radix, folium Artemisiae Argyi, semen Cuscutae, and fructus Ligustri Lucidi (ratio of 1:1:1:1:1), and weight is 1.0%; d-3 is the extract composition of radix Sophorae Flavescentis, rhizoma Ligustici Chuanxiong root, rhizoma Kaempferiae root, Glycyrrhrizae radix, Mandarin orange pericarp, and fructus Zanthoxyli (ratio of 1:1:1:1:1:1), and weight is 0.6%; d-4 is the extract composition of cacumen Platycladi, Polygoni Multiflori radix, folium Artemisiae Argyi, semen Cuscutae, fructus Ligustri Lucidi, radix Sophorae Flavescentis, rhizoma Ligustici Chuanxiong, rhizoma Kaempferiae, Glycyrrhrizae radix, Mandarin orange pericarp, and fructus Zanthoxyli (ratio is 1:1:1:1:1: 1:1:1:1), and weight is 1.1%; d-5 is the extract composition of cacumen Platycladi, Polygoni Multiflori radix, folium Artemisiae Argyi, semen Cuscutae, fructus Ligustri Lucidi, radix Sophorae Flavescentis, rhizoma Ligustici Chuanxiong, rhizoma Kaempferiae, Glycyrrhrizae radix, Mandarin orange pericarp, and fructus Zanthoxyli (ratio is 1:1:1:1:1: 1:1:1:1), and weight is 2.2%;

the operation is as follows: in the embodiment 1, the phase A component is heated to 80 ℃ (± 1 ℃), stirred for 30min, cooled to 40 ℃, and then the phase B component is added, so that the shampoo containing the fibroin Chinese medicinal composition with the anti-hair loss and hair-fixing effects is obtained.

TABLE 5 results of hair loss prevention and hair growth promotion in example 1 and comparative examples 1 to 5

	Example 1	Comparative example 1	Comparative example 2	Comparative example 3	Comparative example 4	Comparative example 5
							The total effective rate%	64.0	68.0	75	64.4	84.4	78.6

From table 5, it can be seen that the anti-hair loss ability sequence is: example 1 < comparative example 3< comparative example 1 < comparative example 2 < comparative example 5 < comparative example 4.

The success rate of delaying hair loss and promoting hair growth of the traditional Chinese medicine composition is only 0.5% in comparative example 3, which is only 0.4% higher than that of example 1, and the success rate of delaying hair loss and promoting hair growth of the traditional Chinese medicine composition is higher than that of single example 1 and comparative example 3, the comparative example 4 and comparative example 5 are different in that one silk protein liquid is used, which is not the silk protein liquid but the traditional Chinese medicine components are doubled, but the comparison of the two shows that the effect of the silk protein liquid is better than that of the traditional Chinese medicine components, which shows that the keratin in the silk protein liquid is more attached to the cutin of the scalp, and the comparative example 4 and comparative example 5 also show that the silk protein liquid is matched with different traditional Chinese medicine extracts, so that the hair loss delaying or promoting hair growth is further improved, the hair growth promoting effect of the silk protein liquid is further improved, the hair growth promoting effect of the hair follicle stimulating effect of the hair cells is improved, the hair growth promoting effect of the hair is improved, and the hair growth promoting effect of the hair is improved by the silk protein liquid, the hair growth promoting hair, and the hair growth promoting effect of the hair is improved by 5 α.

Meanwhile, experiments show that the reasonable matching of the components has important influence on the exertion of the efficacy, and the synergistic effect can be generated only by the reasonable matching of the components, so that the efficacy of preventing hair loss and fixing hair of the invention is achieved.

It can be found by comparison of experimental examples that the specific combination and addition of the amount of the composition of the present invention within the scope of the claims has unique and distinct effects on preventing hair loss and promoting hair growth, which is different from any product and technical scheme disclosed in the prior art.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.

Claims

1. The anti-hair loss and hair-fixing shampoo composition containing fibroin is characterized by comprising the following raw material components in percentage by weight:

2. The fibroin-containing hair loss prevention and hair solidification shampoo composition as claimed in claim 1, wherein: the content range of the traditional Chinese medicine extract is 0.1-10%; the preferred range is 1.0-5.0%.

3. The fibroin-containing hair loss prevention and hair-fixing shampoo composition as claimed in claim 2, wherein: the Chinese medicinal extract is selected from:

0.1-1.0% of Chinese arborvitae extract, 0.1-1.0% of polygonum multiflorum extract, 0.1-1.0% of south dodder seed extract, 0.1-1.0% of wormwood extract, 0.1-1.0% of privet extract, 0.1-1.0% of sophora flavescens extract, 0.1-1.0% of ligusticum wallichii extract, 0.1-1.0% of rhizoma kaempferiae root extract, 0.1-1.0% of liquorice extract, 0.1-1.0% of citrus peel extract and 0.1-1.0% of zanthoxylum bungeanum fruit extract or a mixture of the above.

4. The fibroin-containing hair loss prevention and hair solidification shampoo composition as claimed in claim 1, wherein: the fibroin solution is prepared by fresh cut cocoons by adopting the latest O-discharge double-circulation biochemical hydrolysis technology, controlling the hydrolysis degree of silk through hydrolytic enzyme, disorderly winding protein bundles of the silk, cutting the protein bundles into proteins with different grades and molecular weights, and combining the proteins to obtain the fibroin solution.

5. The hair loss prevention and hair solidification shampoo composition containing fibroin according to claim 4, wherein: by controlling the hydrolysis end point, the molecular weight of the fibroin of the obtained final product is controlled within the range of 2-20 ten thousand daltons, and the molecular weight is mainly centralized within the range of 10-20 ten thousand daltons.

6. The fibroin-containing hair loss prevention and hair solidification shampoo composition as claimed in claim 2, which is prepared from the following raw material components in parts by weight:

7. The fibroin-containing hair loss prevention and hair solidification shampoo composition as claimed in claim 2, which is prepared from the following raw material components in parts by weight:

8. A process for preparing a fibroin-containing anti-hair loss and hair-fixing shampoo composition as defined in any one of claims 1 to 7, comprising the steps of:

9. The method for preparing the hair loss prevention and hair solidification shampoo composition containing the fibroin according to claim 3, which comprises the following steps: