CN110812310B

CN110812310B - Deer fetus extract and composition for preventing alopecia, nourishing and growing hair, and preparation method and application thereof

Info

Publication number: CN110812310B
Application number: CN201911114340.6A
Authority: CN
Inventors: 郭智华; 徐双华; 田冉
Original assignee: Rongzhi Fengsheng Pharmaceutical Co ltd
Current assignee: Rongzhi Fengsheng Pharmaceutical Co ltd
Priority date: 2019-11-14
Filing date: 2019-11-14
Publication date: 2022-10-25
Anticipated expiration: 2039-11-14
Also published as: CN110812310A

Abstract

The invention relates to the technical field of daily cosmetic processing, in particular to a deer fetus extract and a composition for preventing alopecia, nourishing and growing hair, and a preparation method and application thereof. The invention provides a preparation method of a deer fetus extract, which comprises the step of carrying out two-step enzymolysis treatment on pretreated deer fetus by bromelain and composite protease consisting of chymotrypsin and elastase. The method can efficiently hydrolyze to obtain small molecule bioactive substance with effects of preventing alopecia and promoting hair growth, so that effective components in embryo Cervi extract can be easily absorbed by scalp, and can effectively nourish scalp and hair root; the composition obtained by matching the deer fetus extract obtained by the method with the ginger extract, the wheat germ extract and the red ginseng extract has the advantages that the components are coordinated and matched, the scalp and hair root growth cells can be effectively excited, the hair growth blood microcirculation is activated, sufficient nutrients are provided for scalp hair follicles, alopecia is prevented, and the hair is nourished and grown, and the safety is higher.

Description

Deer fetus extract and composition for preventing alopecia, nourishing and growing hair, and preparation method and application thereof

Technical Field

The invention relates to the technical field of daily cosmetic processing, in particular to a deer fetus extract with functions of preventing alopecia, nourishing and growing hair, a composition, a preparation method and application thereof.

Background

Alopecia is a common disease in dermatology, with the development of society, the pressure of life and work of people is greater, the phenomena of alopecia and hair loss are more serious, and the signs of youthfulness gradually appear. Clinical medicine explains the cause of alopecia in many ways, and the reason is that the hair is not grown any more due to atrophy and degeneration of hair follicles. From this point of view, various drugs have been used to induce the growth of the degenerated hair follicle and to extend its anagen phase. The most commonly used drugs for treating alopecia at present include minoxidil and finasteride, etc., which have side effects although they are effective. The treatment effect of the minoxidil is not achieved at once, obvious hair growth can be realized only by long-term adherence during use, and the phenomenon of alopecia rebounds after the medicine is stopped for several months; moreover, drug anaphylaxis may occur in the using process of minoxidil, so that local pruritus, redness and swelling and the like are caused. The side effects of finasteride include gynecomastia, breast enlargement, breast tenderness, erectile dysfunction, testicular pain, abnormal ejaculation, etc., and the administration of a large dose for a long time can weaken the strength of muscles to cause osteoporosis, and the administration of finasteride for women in lactation and pregnancy is prohibited; meanwhile, the efficacy of finasteride for treating alopecia is reversible, patients need to take the medicine for life, and once the medicine is stopped, the hair of the patients can continue to even aggravate the falling within 12 months.

The traditional Chinese medicine holds that the alopecia is related to liver and kidney deficiency, spleen deficiency and excessive dampness, qi and blood deficiency and qi and blood stasis, and the traditional Chinese medicines with the functions of securing essence and tonifying the kidney, promoting blood circulation to remove blood stasis, tonifying qi and nourishing blood and tonifying the liver and kidney are mostly adopted to treat the alopecia, for example, the fructus psoraleae can nourish blood and yin, secure the kidney and blacken the hair; radix Polygoni Multiflori Preparata has effects of arresting seminal emission, invigorating kidney, promoting blood circulation, removing blood stasis, nourishing blood, and nourishing liver and kidney. Compared with western medicines, the traditional Chinese medicine has small side effect and high safety, but has relatively slow effect, and can have obvious effect after being used for a long time.

The deer products such as pilose antler, deer horn, deer skin, deer fetus and the like are recorded in ancient books such as Shennong's herbal classic, ben Cao gang mu and Ben Jing Yuan, and detailed record and explanation are made on the preparation method, the function and the main treatment of the deer products. The deer placenta has the efficacies of tonifying kidney and yang, tonifying deficiency and producing sperm in traditional Chinese medicine, and is also widely used as a tonic in folk. Modern medical research shows that the deer placenta has the effects of regulating immunity, resisting oxidation, delaying senility, benefiting qi, nourishing blood, warming channel, dispelling cold and the like. Chinese patent CN 102872266A discloses a deer fetus compound medicinal preparation which has the function of improving the immunity activity. Chinese patent CN1799590A discloses a deer fetus soft capsule which has the functions of nourishing, health care, body building and body strengthening. At present, no report about the application of the deer fetus extract in cosmetics or external medicines with the functions of preventing alopecia and promoting hair growth exists.

Disclosure of Invention

In order to solve the technical problems in the prior art, the invention aims to provide a deer fetus extract with functions of preventing alopecia and nourishing and growing hair, a preparation method thereof, a composition containing the deer fetus extract and used for preventing alopecia and nourishing and growing hair, and a corresponding product thereof.

In order to achieve the purpose, the invention develops the research and development work of the traditional Chinese medicine components with the functions of efficiently preventing alopecia and growing hair. When the traditional Chinese medicine components with the functions of preventing alopecia and growing hair are externally applied to the skin to exert the effects, the requirements on the content of functional substances and the percutaneous absorption performance are high, and particularly when the traditional Chinese medicine components are applied in the form of products such as shampoo and the like, the key for realizing efficient absorption and utilization within the short contact time with the scalp is that whether the traditional Chinese medicine components can really exert the effects or not. In the research and development process, the deer fetus extract prepared by the enzymolysis method disclosed by the prior art has certain functions of preventing alopecia and growing hair, but when the deer fetus extract is used as a product component externally applied to skin, the functions of preventing alopecia and growing hair are limited, and the improvement is still needed. According to the invention, the preparation process of the deer fetus extract is improved and optimized, a two-step enzymolysis preparation method is developed, the obtained deer fetus extract has rich micromolecule active ingredients and excellent absorption effect through scalp, and the functions of preventing alopecia and promoting hair growth are obviously improved. The deer fetus extract is further compounded with specific traditional Chinese medicine components to prepare the composition with excellent functions of preventing alopecia and growing hair.

Specifically, the technical scheme of the invention is as follows:

firstly, the preparation method of the deer fetus extract comprises the following steps: carrying out two-step enzymolysis treatment on the pretreated deer fetus; wherein, the enzyme used in the first step of enzymolysis treatment comprises bromelain; the enzyme used in the second step of enzymolysis treatment is compound protease, and the compound protease comprises chymotrypsin and elastase.

According to the invention, through a large amount of screening and combination of various enzymes, the two-step enzymolysis method matched with the protease is found to be more favorable for hydrolyzing and releasing small molecular active ingredients in deer tires, so that the scalp absorption efficiency of the active ingredients is improved, and the alopecia prevention and hair growth effects of the deer tires are further effectively improved.

The deer fetus is the placenta of a deer animal. Preferably placenta of Cervus Nippon Temminck.

In the two-step enzymolysis method, the content of active ingredients in the deer fetus extract and the scalp absorption efficiency can be improved more favorably by controlling the dosage ratio of chymotrypsin to elastase in the compound protease, and the deer fetus extract is promoted to play roles in preventing alopecia and growing hair. Preferably, in the compound protease, the dosage ratio of the chymotrypsin to the elastase is 1 (0.3-6) in terms of activity unit. More preferably 1: (0.5-5).

Preferably, in the first step of enzymolysis treatment, the usage amount of the bromelain is 1000-3000U per gram of the pretreated deer fetus, and the enzymolysis treatment time is 2-4 h. In the second step of enzymolysis treatment, the dosage of the compound protease is 2000-4000U per gram of the pretreated deer fetus, and the enzymolysis treatment time is 0.8-2.5 h.

Specifically, the dosage of the chymotrypsin is 500-2500U added to each gram of the pretreated deer fetus; the dosage of the elastase is 1000-4000U per gram of the pretreated deer fetus.

Preferably, the temperature of the first step of enzymolysis treatment is 40-50 ℃; the temperature of the second step of enzymolysis treatment is 20-40 ℃.

In the present invention, the pretreatment of deer fetus preferably adopts a method comprising the following steps: soaking and cleaning deer fetus with normal saline, and adding deionized water in an amount of 4-6 times of the weight of the deer fetus for homogenization treatment.

As a preferable scheme of the invention, the preparation method of the deer fetus extract comprises the following steps:

(1) Pretreating deer fetus: cleaning embryo Cervi in normal saline (0.9% NaCl solution), soaking in normal saline to remove blood stasis, dewatering, draining, cutting into small pieces, adding 4-6 times of deionized water, and homogenizing with colloid mill for 6-10 min to obtain embryo Cervi suspension;

(2) The first step of enzymolysis treatment: performing first-step enzymolysis on the deer fetus suspension obtained in the step (1) by adopting bromelain, wherein the dosage of the bromelain is 1000-3000U/g of the deer fetus suspension, the enzymolysis temperature is 40-50 ℃, and the enzymolysis time is 3-4 h, so as to obtain first-step enzymolysis liquid;

(3) The second step of enzymolysis treatment: and after the temperature of an enzymolysis system is lower than 40 ℃, adding compound protease into the first-step enzymolysis liquid for carrying out second-step enzymolysis, wherein the dosage of the compound protease is 2000-4000U/g deer fetus suspension, and the compound protease is prepared by mixing the components of 1: (0.5-5) chymotrypsin and elastase; the enzymolysis temperature is 20-40 ℃, and the enzymolysis time is 0.8-1.5 h; and centrifuging the solution obtained after the second step of enzymolysis to obtain a supernatant.

Further, the invention provides a deer fetus extract, which is prepared by the preparation method of the deer fetus extract.

According to the regulations of the European Commission, the internationally named cosmetic raw material (INCI) for deer fetus extract is PLACENTAL PROTEIN, PLACENTAL ENZYMES.

Furthermore, the invention provides a hair-loss-preventing, nourishing and hair-growing composition which comprises the deer fetus extract prepared by the preparation method of the deer fetus extract.

Preferably, the composition further comprises the following components: ginger extract, wheat germ extract, and red ginseng extract.

The invention discovers that the deer fetus extract prepared by the preparation method disclosed by the invention is compatible with the ginger extract, the wheat germ extract and the red ginseng extract for use, the ginger extract, the wheat germ extract and the red ginseng extract can effectively exert the effects of greatly invigorating primordial qi, recovering pulse, fixing desertion, benefiting qi, controlling blood, benefiting qi, culturing primordial qi and the like, and each component can well coordinate and cooperate with each other to exert excellent alopecia prevention and hair growth effects.

In the present invention, the ginger extract, the wheat germ extract and the red ginseng extract may be prepared by a conventional method in the art or may be commercially available.

In the composition, the synergistic cooperation effect of the components can be better promoted by controlling the dosage and the proportion of the deer fetus extract and the wheat germ extract, and the hair loss prevention and hair growth effects of the composition are effectively improved. Preferably, in the composition, the mass ratio of the deer fetus extract to the wheat germ extract is 1: (1-4).

Preferably, the composition comprises the following components in parts by weight: 0.5-4 parts of deer fetus extract, 2-10 parts of ginger extract, 0.1-4 parts of wheat germ extract and 0.2-5 parts of red ginseng extract.

More preferably, the composition comprises the following components in parts by weight: 1-4 parts of deer fetus extract, 4-10 parts of ginger extract, 1-4 parts of wheat germ extract and 1-5 parts of red ginseng extract.

As a preferable scheme of the invention, the composition comprises the following components in parts by weight: 1-2 parts of deer fetus extract, 2-6 parts of ginger extract, 2-4 parts of wheat germ extract and 2-5 parts of red ginseng extract.

The invention also provides a deer fetus extract or a preparation method of the deer fetus extract or application of the alopecia preventing and hair growing composition in preparing products with alopecia preventing or nourishing and hair growing functions.

Preferably, the product is a cosmetic or a topical medicament; the deer fetus extract is prepared by the preparation method of the deer fetus extract.

The cosmetics include, but are not limited to, shampoo, hair tonic, hair dye, hair conditioner, hair style treatment, hair cream, hair tonic lotion.

The external medicine includes, but is not limited to, scalp therapeutic agent, ointment.

The invention provides shampoo which comprises the composition for preventing hair loss, nourishing and growing hair.

Preferably, the hair loss preventing, nourishing and hair growing composition is 2-15% by mass of the shampoo.

The shampoo also comprises shampoo bases allowed in the field.

Preferably, the shampoo base may comprise one or more components selected from the group consisting of: 10 to 20 portions of alkyl glycoside, 6 to 15 portions of anionic surfactant, 5 to 10 portions of zwitterionic surfactant, 2 to 5 portions of amino acid humectant, 2 to 5 portions of glycerol, 1 to 3 portions of polyquaternium, 0.1 to 0.5 portion of citric acid, 0.1 to 0.5 portion of EDTA-2Na, 0.5 to 2.0 portions of sodium chloride, 0.05 to 0.5 portion of essence, 0.01 to 0.1 portion of preservative and 40 to 80 portions of deionized water.

Wherein the anionic surfactant is one or more of sodium lauroyl sarcosinate, fatty alcohol-polyoxyethylene ether sodium sulfate, ammonium dodecyl sulfate and sodium dodecyl benzene sulfonate; the zwitterionic surfactant is one or more of cocamidopropyl betaine, dodecyl dimethyl betaine and imidazoline.

The invention also provides a hair growth lotion which comprises the hair loss preventing, nourishing and hair growth composition.

Preferably, the hair-loss preventing, nourishing and hair-growing composition is 2-15% by mass of the hair-loss preventing, nourishing and hair-growing water.

The shampoo also comprises a hair growth promoting and hydrating matrix allowed in the field.

Preferably, the hair growth and water retention matrix may comprise one or more selected from the group consisting of: 5-15 parts of glycerol, 2-6 parts of sweet orange oil, 2-8 parts of salicylic acid, 1-5 parts of ethanol and 0.01-0.1 part of menthol.

The invention has the beneficial effects that:

(1) The preparation method of the deer fetus extract provided by the invention can efficiently hydrolyze deer fetus to obtain the micromolecule bioactive substance with the effects of preventing alopecia and growing hair, so that the functional components in the deer fetus extract are easier to be absorbed by scalp, and the scalp and hair roots are effectively nourished; the deer fetus extract prepared by the preparation method of the deer fetus extract provided by the invention has excellent functions of preventing alopecia, nourishing and growing hair;

(2) The composition obtained by matching the deer fetus extract, the ginger extract, the wheat germ extract and the red ginseng extract can well coordinate and cooperate with each other, promote the growth and proliferation of hair follicles and hair follicle dermal-derived cells and epidermal cells, induce the hair cycle from a resting period to a growing period, and maintain the hair cycle of the growing period for a longer time; the shampoo and hair tonic prepared from the composition can activate hair growth microcirculation, provide sufficient nutrients for scalp hair follicles, promote healthy growth of hair, and prevent alopecia and nourish hair;

(3) Compared with the common anti-hair-loss drugs (minoxidil, finasteride and the like), the anti-hair-loss and hair-growth composition provided by the invention has smaller side effect and higher safety.

Detailed Description

Preferred embodiments of the present invention will be described in detail with reference to the following examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and alterations of this invention will become apparent to those skilled in the art without departing from the spirit and scope of this invention.

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Materials, reagents, etc. used in the following examples are commercially available, unless otherwise specified, wherein Bromelain is purchased from Sigma-Aldrich (Bromelain), chymotrypsin is purchased from Amresco (Amresco M102), and embryo cervi extract described in the following examples is LORICH.

The preparation of the various plant extracts used in the following examples was as follows:

the preparation method of the ginger extract comprises the following steps: selecting ginger with plump root blocks and no mildew or rot, cleaning soil with drinking water, slicing the cleaned ginger, adding water 1 time the weight of the ginger, grinding for 2 minutes to obtain homogenate, filtering the homogenate with 100-mesh filter cloth to obtain filtrate, standing the filtrate overnight, and taking supernatant to obtain the ginger extract.

The preparation method of the wheat germ extract comprises the following steps: crushing and sieving fresh wheat germs by a swing type high-speed traditional Chinese medicine crusher (100 meshes) to obtain fine powder, and mixing the fine powder with deionized water according to a liquid-material ratio of 10; extracting under stirring for 3 hr, centrifuging at low temperature (10000 r/min,4 deg.C, 20 min) to obtain supernatant, extracting the residue with deionized water for 1 hr, centrifuging under the same conditions, and mixing the supernatants to obtain wheat germ extract.

The preparation method of the red ginseng extract comprises the following steps: slicing Ginseng radix Rubri, soaking in 8 times of drinking water for 1 hr, heating to boil, extracting for three times (1.5 hr for each time) with 8 times of drinking water, filtering extractive solutions, mixing filtrates, standing overnight, and collecting supernatant to obtain Ginseng radix Rubri extract.

Example 1 preparation method of deer fetus extract (1)

The embodiment provides a preparation method of a deer fetus extract, which comprises the following specific steps:

(1) Pretreating deer fetus: melting embryo Cervi in normal saline, cleaning, soaking in normal saline to remove blood stasis, dewatering, draining, cutting into small pieces, adding 6 times of deionized water, and homogenizing with colloid mill for 8min to obtain embryo Cervi suspension;

(2) The first step of enzymolysis: performing first-step enzymolysis on the deer fetus suspension by adopting bromelain, wherein the addition amount of the bromelain is 3000U/g of the deer fetus suspension, the enzymolysis temperature is 40 ℃, and the enzymolysis time is 3 hours, so as to obtain first-step enzymolysis liquid;

(3) The second step of enzymolysis: after the temperature of an enzymolysis system is lower than 40 ℃, adding compound protease (chymotrypsin: elastase with a mass ratio of 1:5) into the first-step enzymolysis liquid to carry out second-step enzymolysis, wherein the addition amount of the compound protease is 4000U/g, the enzymolysis temperature is 20 ℃, and the enzymolysis time is 1h; centrifuging the solution after enzymolysis, and separating supernatant to obtain the final product.

The present embodiment also provides a deer fetus extract prepared by the above preparation method.

Example 2 preparation method of deer fetus extract (2)

(2) The first step of enzymolysis: performing first-step enzymolysis on the deer fetus suspension by adopting bromelain, wherein the addition amount of the bromelain is 1000U/g of the deer fetus suspension, the enzymolysis temperature is 50 ℃, and the enzymolysis time is 3 hours, so as to obtain first-step enzymolysis liquid;

(3) The second step of enzymolysis: after the temperature of an enzymolysis system is lower than 40 ℃, adding compound protease (chymotrypsin: elastase with a mass ratio of 1:1) into the first-step enzymolysis liquid to carry out second-step enzymolysis, wherein the addition amount of the compound protease is 2000U/g, the enzymolysis temperature is 40 ℃, and the enzymolysis time is 1h; centrifuging the solution after enzymolysis, and separating supernatant to obtain the final product.

Example 3 preparation of deer fetus extract (3)

(2) The first step of enzymolysis: performing first-step enzymolysis on the deer fetus suspension by adopting bromelain, wherein the addition amount of the bromelain is 2000U/g of the deer fetus suspension, the enzymolysis temperature is 45 ℃, and the enzymolysis time is 3 hours, so as to obtain first-step enzymolysis liquid;

(3) The second step of enzymolysis: after the temperature of an enzymolysis system is lower than 40 ℃, adding compound protease (chymotrypsin: elastase mass ratio of 2:1) into the first-step enzymolysis liquid to carry out second-step enzymolysis, wherein the addition amount of the compound protease is 3000U/g, the enzymolysis temperature is 30 ℃, and the enzymolysis time is 1h; centrifuging the solution after enzymolysis, and separating supernatant to obtain the final product.

Example 4 preparation method of deer fetus extract (4)

(3) The second step of enzymolysis: after the temperature of an enzymolysis system is lower than 40 ℃, adding compound protease (chymotrypsin: elastase mass ratio is 1: 2.5) into the first-step enzymolysis liquid to carry out second-step enzymolysis, wherein the addition amount of the compound protease is 3000U/g, the enzymolysis temperature is 30 ℃, and the enzymolysis time is 1h; centrifuging the solution after enzymolysis, and separating supernatant to obtain the final product.

Example 5 Hair growth composition for preventing alopecia and nourishing Hair (1)

The embodiment provides a composition for preventing alopecia, nourishing and growing hair, which comprises the following components: 2 parts of deer fetus extract, 6 parts of ginger extract, 2 parts of wheat germ extract and 2 parts of red ginseng extract, which are prepared in example 1.

Example 6 Hair growth composition for preventing alopecia and nourishing Hair (2)

The embodiment provides a composition for preventing alopecia, nourishing and growing hair, which comprises the following components: 2 parts of deer fetus extract, 6 parts of ginger extract, 2 parts of wheat germ extract and 2 parts of red ginseng extract, which are prepared in example 2.

Example 7 Hair growth composition for preventing alopecia and nourishing Hair (3)

The embodiment provides a composition for preventing alopecia, nourishing and growing hair, which comprises the following components: 2 parts of deer fetus extract, 6 parts of ginger extract, 2 parts of wheat germ extract and 2 parts of red ginseng extract, which are prepared in example 3.

Example 8 Hair growth composition for preventing alopecia and nourishing (4)

The embodiment provides a composition for preventing alopecia, nourishing and growing hair, which comprises the following components: 2 parts of deer fetus extract, 6 parts of ginger extract, 2 parts of wheat germ extract and 2 parts of red ginseng extract, which are prepared in example 4.

Example 9 Hair loss prevention, nourishing Hair growth composition (5)

The embodiment provides a composition for preventing alopecia, nourishing and growing hair, which comprises the following components: example 4 the prepared deer fetus extract 1 part, ginger extract 2 parts, wheat germ extract 4 parts, and red ginseng extract 5 parts.

Example 10 Hair loss prevention shampoo (1)

The embodiment provides an anti-hair loss shampoo, which comprises the composition of embodiment 5, and specifically comprises the following components: 2 parts of deer fetus extract prepared in example 1, 6 parts of ginger extract, 2 parts of wheat germ extract, 2 parts of red ginseng extract and 88 parts of shampoo matrix.

The shampoo matrix comprises the following components:

10% of alkyl glycoside, 8% of sodium lauroyl sarcosinate, 8% of cocamidopropyl betaine, 2% of amino acid humectant, 2% of glycerol, 0.1% of essence and 69.9% of deionized water.

The embodiment also provides a preparation method of the shampoo, which comprises the following steps: adding the deer fetus extract, the ginger extract, the wheat germ extract and the red ginseng extract prepared in example 1 into a shampoo base, and uniformly stirring to obtain the shampoo base.

Example 11 Hair loss prevention shampoo (2)

The embodiment provides an anti-hair loss shampoo, which comprises the composition of embodiment 6, and the specific composition is as follows: 2 parts of deer fetus extract, 6 parts of ginger extract, 2 parts of wheat germ extract, 2 parts of red ginseng extract and 88 parts of shampoo matrix prepared in example 2.

The shampoo base was the same as in example 10.

The embodiment also provides a preparation method of the shampoo, which comprises the following steps: adding the deer fetus extract, the ginger extract, the wheat germ extract and the red ginseng extract prepared in example 2 into a shampoo base, and uniformly stirring to obtain the shampoo base.

Example 12 Hair loss prevention shampoo (3)

The embodiment provides an anti-hair loss shampoo, which comprises the composition of embodiment 7 and specifically comprises the following components: 2 parts of deer fetus extract, 6 parts of ginger extract, 2 parts of wheat germ extract, 2 parts of red ginseng extract and 88 parts of shampoo matrix prepared in example 3.

The shampoo base was the same as in example 10.

The embodiment also provides a preparation method of the shampoo, which comprises the following steps: adding the deer fetus extract, the ginger extract, the wheat germ extract and the red ginseng extract prepared in example 3 into a shampoo base, and uniformly stirring to obtain the shampoo base.

Example 13 Hair loss prevention shampoo (4)

The embodiment provides an anti-hair loss shampoo, which comprises the composition of embodiment 8 and specifically comprises the following components: 2 parts of deer fetus extract, 6 parts of ginger extract, 2 parts of wheat germ extract, 2 parts of red ginseng extract and 88 parts of shampoo matrix prepared in example 4.

The shampoo base was the same as in example 10.

The embodiment also provides a preparation method of the shampoo, which comprises the following steps: adding the deer fetus extract, the ginger extract, the wheat germ extract and the red ginseng extract prepared in example 4 to a shampoo base, and uniformly stirring to obtain the shampoo base.

Example 14 Hair loss prevention shampoo (5)

The embodiment provides a shampoo for preventing hair loss, which comprises the composition of embodiment 9, and the specific composition is as follows: the deer fetus extract prepared in example 4 comprises 1 part of deer fetus extract, 2 parts of ginger extract, 4 parts of wheat germ extract, 5 parts of red ginseng extract and 88 parts of shampoo matrix.

The shampoo base was the same as in example 10.

The embodiment also provides a preparation method of the shampoo, which comprises the following steps: adding the deer fetus extract, the ginger extract, the wheat germ extract and the red ginseng extract prepared in example 4 into a shampoo base, and uniformly stirring to obtain the shampoo base.

Example 15 Hair restorer (1)

The embodiment provides a hair tonic, which comprises the composition of embodiment 8, and the specific composition is as follows: 2 parts of deer fetus extract prepared in example 4, 6 parts of ginger extract, 2 parts of wheat germ extract, 2 parts of red ginseng extract, and 88 parts of hair growth promoting water matrix.

Wherein, the hair growth water matrix comprises the following components: 10% of glycerol, 3% of sweet orange oil, 5% of ethanol and 82% of deionized water.

The embodiment also provides a preparation method of the hair restorer, which comprises the following steps: adding the deer fetus extract, the ginger extract, the wheat germ extract and the red ginseng extract prepared in example 4 to a hair growth promoting aqueous medium, and stirring uniformly.

EXAMPLE 16 Hair growth Water (2)

This example provides a hair tonic comprising the composition of example 9, consisting essentially of: 1 part of deer fetus extract prepared in example 4, 2 parts of ginger extract, 4 parts of wheat germ extract, 5 parts of red ginseng extract and 88 parts of hair growth promoting water matrix.

Wherein, the composition of the hair growth promoting base is the same as that of example 15.

The embodiment also provides a preparation method of the hair restorer, which comprises the following steps: adding the deer fetus extract, the ginger extract, the wheat germ extract and the red ginseng extract prepared in example 4 into a hair growth promoting aqueous medium, and uniformly stirring to obtain the hair growth promoting composition.

Comparative example 1

The comparative example provides a preparation method of deer fetus extract, which comprises the following specific steps:

(2) Enzymolysis: performing enzymolysis on embryo Cervi suspension with bromelain at an addition of 2000U/g embryo Cervi suspension at 45 deg.C for 3 hr, centrifuging the solution, and separating supernatant.

Comparative example 2

(3) The second step of enzymolysis: after the temperature of an enzymolysis system is lower than 40 ℃, adding compound protease (chymotrypsin: trypsin mass ratio is 1; centrifuging the solution after enzymolysis, and separating supernatant to obtain the final product.

Comparative example 3

The comparative example provides a shampoo, which comprises the following specific components: 2 parts of deer fetus extract, 6 parts of ginger extract, 2 parts of wheat germ extract, 2 parts of red ginseng extract and 88 parts of shampoo matrix prepared in comparative example 1.

The composition of the shampoo base and the preparation method of the shampoo were the same as in example 13.

Comparative example 4

The comparative example provides shampoo which comprises the following specific components: 2 parts of deer fetus extract prepared in comparative example 2, 6 parts of ginger extract, 2 parts of wheat germ extract, 2 parts of red ginseng extract and 88 parts of shampoo matrix.

Comparative example 5

The comparative example provides a shampoo, which comprises the following specific components: 7 parts of ginger extract, 2.5 parts of wheat germ extract, 2.5 parts of red ginseng extract and 88 parts of shampoo matrix.

Comparative example 6

The comparative example provides shampoo which comprises the following specific components: 2 parts of cacumen biotae extract, 6 parts of ginger extract, 2 parts of wheat germ extract, 2 parts of red ginseng extract and 88 parts of shampoo matrix.

The composition of the shampoo base and the preparation of the shampoo were the same as in example 13.

Comparative example 7

The comparative example provides a shampoo, which comprises the following specific components: 2 parts of deer fetus extract, 6 parts of ginger extract, 2 parts of wild soybean extract, 2 parts of red ginseng extract and 88 parts of shampoo matrix in example 4.

Comparative example 8

The comparative example provides shampoo which comprises the following specific components: shampoo base 100 parts, the composition of shampoo base is the same as example 10.

Comparative example 9

The comparative example provides a hair tonic, which specifically consists of: the composition of the hair growth promoting base is the same as that of example 15 with 100 parts of the hair growth promoting base.

Experimental example 1 skin absorption Effect of deer fetus extract

This experimental example analyzes the skin absorption effect of the deer fetus extracts prepared in examples 1 to 4, and the specific method is as follows:

experimental animals: c57BL/6 mice, weight 18-22g, total 40, random into four groups, each group of 10.

Depilating mouse with 8% sodium sulfide for 30s, washing with normal saline, peeling skin, removing subcutaneous adipose tissue, washing with normal saline, fixing the pretreated skin between diffusion chamber and receiving chamber of osmotic diffusion device, and injecting 17mL of normal saline as receiving solution into the receiving chamber via sampling tube to make the liquid surface completely contact with skin inner layer; the deer fetus extracts prepared in examples 1 to 4 were added into the diffusion chamber, and the top of the diffusion chamber and the sampling port of the receiving chamber were covered with a preservative film. Continuously stirring, sampling after 1h, and detecting the polypeptide content in the deer fetus extract in the diffusion chamber before and after stirring by using a Coomassie brilliant blue kit. The test results are shown in table 1, and the results show that the polypeptide content of each solution after stirring is significantly lower than that before stirring, which indicates that the deer fetus extracts prepared in examples 1 to 4 have better effect of skin permeation and absorption.

TABLE 1 detection of polypeptide content in solution

Examples	Polypeptide content of solution before stirring	Polypeptide content of the solution after stirring
			Example 1	1.2％	0.7％
Example 2	1.1％	0.6％
			Example 3	1.3％	0.8％
Example 4	1.4％	0.6％

Experimental example 2 analysis of Hair loss prevention Effect of shampoo

In order to verify the hair loss prevention effect of the shampoos in the examples, 110 volunteers with alopecia between 25-60 years old were selected and divided into 11 groups of 10 persons, each group provided with one of the shampoos in examples 10-14 and comparative examples 3-8, respectively, for 3 months, and the frequency of use was once every two days. The results of the statistics of the number of hairs shed before use and after 3 months of use, the number of hairs shed 1 day before use (average of 10 persons per group) and the number of hairs shed 1 day after 3 months of use (average of 10 persons per group) are shown in Table 2, by combing the hairs 3 times a day, in the morning, in the middle and in the evening, and recording the number of hairs shed.

TABLE 2 statistics of alopecia prevention Effect

Experimental sample	Number of hair falling before use (root/day)	Hair loss after 3 months of use (root/day)
			Example 10	103	70
Example 11	100	60
			Example 12	101	73
Example 13	106	53
			Example 14	103	57
Comparative example 3	102	83
			Comparative example 4	100	80
Comparative example 5	102	90
			Comparative example 6	101	86
Comparative example 7	102	70
			Comparative example 8	105	103

As can be seen from table 2, the hair loss prevention function of the shampoos (examples 10 to 14) containing the deer fetus extracts prepared in examples 1 to 4 was significantly improved as compared to the shampoos of each comparative example, and among them, the shampoos (examples 13 and 14) containing the deer fetus extracts prepared in example 4 had more excellent hair loss prevention effect.

Experimental example 3 Hair growth effect analysis of Hair lotion

This example analyzes the hair tonic effect of examples 15 and 16, and the specific method is as follows:

experimental animals: 7-week-old mice (C57 BL/6) were born with good skin on the back and a weight of 18-22g, and 30 mice were randomly divided into 3 groups of 10 mice each.

After the hair on the back of the mice was shaved, 150. Mu.L of the hair-growing water of example 15, example 16 and comparative example 9 described above was applied to the back of each group of mice each day. After 23 days of use, the weight of newly grown hairs was measured and compared, and the results are shown in Table 3 (average hair weight of 10 mice per group).

TABLE 3 comparison of hair growth

Experimental sample	Average weight of hair (mg)
		Example 15	53.1
Example 16	50.3
		Comparative example 9	43.2

As is clear from the results of table 3, when the hair restorers of examples 15 and 16 were used in mice in the resting stage hair cycle, the change to the growth stage hair cycle was more promoted than in comparative example 9, and thus the hair restorers of examples 15 and 16 had an excellent hair growth promoting effect.

Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto without departing from the scope of the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. A composition for preventing hair loss, nourishing and promoting hair growth is characterized by comprising deer fetus extract, ginger extract, wheat germ extract and red ginseng extract;

wherein the preparation method of the deer fetus extract comprises the following steps: carrying out two-step enzymolysis treatment on the pretreated deer fetus; wherein, the enzyme used in the first step of enzymolysis treatment is bromelain; the enzyme used in the second step of enzymolysis treatment is composite protease, and the composite protease consists of chymotrypsin and elastase; in the compound protease, the dosage ratio of the chymotrypsin to the elastase is 1: (0.5 to 5);

in the first step of enzymolysis treatment, the usage amount of the bromelain is 1000 to 3000U per gram of the pretreated deer fetus, the temperature of the enzymolysis treatment is 40 to 50 ℃, and the enzymolysis treatment time is 2 to 4h;

in the second step of enzymolysis treatment, the dosage of the composite protease is 2000-4000U per gram of the pretreated deer fetus, wherein the dosage of the chymotrypsin is 500-2500U per gram of the pretreated deer fetus, and the dosage of the elastase is 1000-4000U per gram of the pretreated deer fetus; the temperature of the enzymolysis treatment is 20 to 40 ℃, and the time of the enzymolysis treatment is 0.8 to 2.5h;

the pretreatment comprises the following steps: soaking and cleaning deer fetus with physiological saline, adding 4~6 times weight of deionized water, and homogenizing.

2. The composition as claimed in claim 1, wherein the mass ratio of deer fetus extract to wheat germ extract in the composition is 1: (1~4).

3. The composition according to claim 1, which comprises the following components in parts by weight: 0.5-4 parts of deer fetus extract, 2-10 parts of ginger extract, 0.1-4 parts of wheat germ extract and 0.2-5 parts of red ginseng extract.

4. The composition of claim 3, wherein the composition comprises the following components in parts by weight: 1-4 parts of deer fetus extract, 4-10 parts of ginger extract, 1-4 parts of wheat germ extract and 1-5 parts of red ginseng extract.

5. Use of the composition of any one of claims 1~4 in the manufacture of a product having hair loss prevention or hair tonic and hair growth promoting properties;

the product is cosmetic or topical medicine.

6. A shampoo comprising the composition of any of claims 1~4.

7. The shampoo according to claim 6, wherein the composition is contained in the shampoo in an amount of 2 to 15% by mass.

8. A hair-growth water comprising the composition of any one of claims 1~4.

9. The hair restorer according to claim 8, wherein the composition is contained in the hair restorer in a mass percentage of 2 to 15%.