CN111344410B - 从宿主细胞半乳糖凝集素和其他污染物中纯化糖基化蛋白的方法 - Google Patents

从宿主细胞半乳糖凝集素和其他污染物中纯化糖基化蛋白的方法 Download PDF

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Publication number
CN111344410B
CN111344410B CN201880052805.7A CN201880052805A CN111344410B CN 111344410 B CN111344410 B CN 111344410B CN 201880052805 A CN201880052805 A CN 201880052805A CN 111344410 B CN111344410 B CN 111344410B
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protein
galectin
buffer
matrix
cations
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CN111344410A (zh
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L·A·康奈尔-克罗利
M·J·麦克卢尔
R·O·吉莱斯皮
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Jishiyiwei Biology Co ltd
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Jishiyiwei Biology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201880052805.7A 2017-08-17 2018-08-17 从宿主细胞半乳糖凝集素和其他污染物中纯化糖基化蛋白的方法 Active CN111344410B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762546558P 2017-08-17 2017-08-17
US62/546,558 2017-08-17
PCT/US2018/046919 WO2019036626A1 (en) 2017-08-17 2018-08-17 PROCESS FOR PURIFYING GLYCOSYLATED PROTEIN FROM GALECTINES AND OTHER HOST CELL CONTAMINANTS

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Publication Number Publication Date
CN111344410A CN111344410A (zh) 2020-06-26
CN111344410B true CN111344410B (zh) 2023-09-15

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Country Link
US (1) US11358983B2 (https=)
EP (1) EP3668985B1 (https=)
JP (1) JP7097433B2 (https=)
KR (1) KR102578087B1 (https=)
CN (1) CN111344410B (https=)
CA (1) CA3067735A1 (https=)
DK (1) DK3668985T3 (https=)
ES (1) ES2887046T3 (https=)
MY (1) MY199833A (https=)
WO (1) WO2019036626A1 (https=)

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DK3668985T3 (da) 2017-08-17 2021-09-20 Just Evotec Biologics Inc Fremgangsmåde til rensning af glycosyleret protein fra værtscellegalectiner og andre urenheder
US11180540B2 (en) * 2019-12-06 2021-11-23 Regeneron Pharmaceuticals, Inc. Anti-VEGF protein compositions and methods for producing the same
JP2023525034A (ja) 2020-05-08 2023-06-14 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ｖｅｇｆトラップおよびミニトラップならびに眼障害およびがんの治療方法
WO2023192926A2 (en) * 2022-03-30 2023-10-05 Ionis Pharmaceuticals, Inc. Methods for separating certain oligonucleotide compounds
TW202502843A (zh) * 2023-03-17 2025-01-16 日商丸善石油化學股份有限公司 聚合物之製造方法

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CN113439118A (zh) * 2019-02-15 2021-09-24 济世易为生物有限公司 自动化生物制造系统、设备和方法

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CN101213211A (zh) * 2005-06-17 2008-07-02 惠氏公司 纯化含Fc区蛋白的方法
CN102711828A (zh) * 2009-10-20 2012-10-03 雅培制药有限公司 使用蛋白a亲和色谱法分离和纯化抗-il-13抗体
CN106536565A (zh) * 2014-06-13 2017-03-22 鲁宾有限公司 一种用于纯化TNFR‑Fc融合蛋白的方法
CN106749660A (zh) * 2016-12-27 2017-05-31 嘉和生物药业有限公司 单克隆抗体下游纯化过程中有效去除宿主蛋白的方法
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Publication number Publication date
EP3668985B1 (en) 2021-06-16
JP2020531411A (ja) 2020-11-05
US20210163528A1 (en) 2021-06-03
JP7097433B2 (ja) 2022-07-07
WO2019036626A1 (en) 2019-02-21
ES2887046T3 (es) 2021-12-21
KR20200037396A (ko) 2020-04-08
US11358983B2 (en) 2022-06-14
MY199833A (en) 2023-11-24
KR102578087B1 (ko) 2023-09-18
EP3668985A1 (en) 2020-06-24
CA3067735A1 (en) 2019-02-21
DK3668985T3 (da) 2021-09-20
CN111344410A (zh) 2020-06-26

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