MY199833A - Method of purifying glycosylated protein from host cell galectins and other contaminants - Google Patents

Method of purifying glycosylated protein from host cell galectins and other contaminants

Info

Publication number
MY199833A
MY199833A MYPI2019007631A MYPI2019007631A MY199833A MY 199833 A MY199833 A MY 199833A MY PI2019007631 A MYPI2019007631 A MY PI2019007631A MY PI2019007631 A MYPI2019007631 A MY PI2019007631A MY 199833 A MY199833 A MY 199833A
Authority
MY
Malaysia
Prior art keywords
galectins
host cell
contaminants
glycosylated protein
purifying
Prior art date
Application number
MYPI2019007631A
Other languages
English (en)
Inventor
Lisa A Connell-Crowley
Megan J Mcclure
Ronald O Gillespie
Original Assignee
Just Evotec Biologics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Just Evotec Biologics Inc filed Critical Just Evotec Biologics Inc
Publication of MY199833A publication Critical patent/MY199833A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
MYPI2019007631A 2017-08-17 2018-08-17 Method of purifying glycosylated protein from host cell galectins and other contaminants MY199833A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762546558P 2017-08-17 2017-08-17
PCT/US2018/046919 WO2019036626A1 (en) 2017-08-17 2018-08-17 PROCESS FOR PURIFYING GLYCOSYLATED PROTEIN FROM GALECTINES AND OTHER HOST CELL CONTAMINANTS

Publications (1)

Publication Number Publication Date
MY199833A true MY199833A (en) 2023-11-24

Family

ID=63684424

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2019007631A MY199833A (en) 2017-08-17 2018-08-17 Method of purifying glycosylated protein from host cell galectins and other contaminants

Country Status (10)

Country Link
US (1) US11358983B2 (https=)
EP (1) EP3668985B1 (https=)
JP (1) JP7097433B2 (https=)
KR (1) KR102578087B1 (https=)
CN (1) CN111344410B (https=)
CA (1) CA3067735A1 (https=)
DK (1) DK3668985T3 (https=)
ES (1) ES2887046T3 (https=)
MY (1) MY199833A (https=)
WO (1) WO2019036626A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3668985T3 (da) 2017-08-17 2021-09-20 Just Evotec Biologics Inc Fremgangsmåde til rensning af glycosyleret protein fra værtscellegalectiner og andre urenheder
US11180540B2 (en) * 2019-12-06 2021-11-23 Regeneron Pharmaceuticals, Inc. Anti-VEGF protein compositions and methods for producing the same
JP2023525034A (ja) 2020-05-08 2023-06-14 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ｖｅｇｆトラップおよびミニトラップならびに眼障害およびがんの治療方法
WO2023192926A2 (en) * 2022-03-30 2023-10-05 Ionis Pharmaceuticals, Inc. Methods for separating certain oligonucleotide compounds
TW202502843A (zh) * 2023-03-17 2025-01-16 日商丸善石油化學股份有限公司 聚合物之製造方法

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
WO1990001063A1 (en) 1988-07-23 1990-02-08 Delta Biotechnology Limited New secretory leader sequences
AU632065B2 (en) 1988-09-23 1992-12-17 Novartis Vaccines And Diagnostics, Inc. Cell culture medium for enhanced cell growth, culture longevity and product expression
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
AT403989B (de) 1996-09-16 1998-07-27 Immuno Ag Verfahren zur herstellung eines plasmaprotein-hältigen arzneimittels
EP0950067B1 (en) 1996-11-27 2007-08-29 Genentech, Inc. Affinity purification of polypeptide on protein a matrix
US6022952A (en) 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
US6660843B1 (en) 1998-10-23 2003-12-09 Amgen Inc. Modified peptides as therapeutic agents
CA2350226C (en) 1998-11-20 2012-04-24 Fuso Pharmaceutical Industries, Ltd. Protein expression vector and utilization thereof
JP2002538841A (ja) 1999-03-18 2002-11-19 ヒューマン ジノーム サイエンシーズ， インコーポレイテッド ２７個のヒト分泌タンパク質
US7306799B2 (en) 1999-06-08 2007-12-11 Regeneron Pharmaceuticals, Inc. Use of VEGF inhibitors for treatment of eye disorders
CN100523187C (zh) 1999-06-08 2009-08-05 里珍纳龙药品有限公司 具有改善的药物动力学特性的修饰嵌合多肽
US7070959B1 (en) 1999-06-08 2006-07-04 Regeneron Pharmaceuticals, Inc. Modified chimeric polypeptides with improved pharmacokinetic properties
US7303746B2 (en) 1999-06-08 2007-12-04 Regeneron Pharmaceuticals, Inc. Methods of treating eye disorders with modified chimeric polypeptides
AU2003210802B2 (en) 2002-02-05 2009-09-10 Genentech Inc. Protein purification
DE602004026343D1 (de) 2003-10-24 2010-05-12 Amgen Inc Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen
TWI372763B (en) * 2005-06-17 2012-09-21 Wyeth Llc Methods of purifying anti a beta antibodies
FR2894146B1 (fr) 2005-12-07 2008-06-06 Ifremer Utilisation d'un polysaccharide excrete par l'espece vibrio diabolicus a des fins d'ingenierie des tissus conjonctifs non mineralises
WO2007109163A2 (en) 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using
US7998927B2 (en) 2006-06-23 2011-08-16 Aegis Therapeutics, Llc Stabilizing alkylglycoside compositions and methods thereof
AU2008206923A1 (en) 2007-01-17 2008-07-24 Merck Serono S.A. Process for the purification of Fc-containing proteins
CL2008002054A1 (es) 2007-07-17 2009-05-29 Hoffmann La Roche Metodo para la regeneracion de una columna de cromatografia de intercambio cationico despues de la elusion de eritropoyetina monopeguilada y metodo para obtener una eritropoyetina monopeguilada, incorporando el metodo de regeneracion de la columna de intercambio cationico.
EP2321338A1 (en) 2008-08-14 2011-05-18 Merck Sharp & Dohme Corp. Methods for purifying antibodies using protein a affinity chromatography
AU2009347206C1 (en) 2008-10-20 2016-12-08 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
BRPI0919879A2 (pt) 2008-10-29 2016-02-16 Wyeth Llc métodos para purificação de moléculas de ligação a antígeno de domínio único
WO2011038894A1 (en) 2009-10-01 2011-04-07 F. Hoffmann-La Roche Ag Protein a chromatography
ES2813398T3 (es) * 2009-10-20 2021-03-23 Abbvie Inc Aislamiento y purificación de anticuerpos anti-IL-13 usando cromatografía de afinidad a Proteína A
EP2513134B1 (en) 2009-12-18 2017-09-06 Novartis AG Wash solution and method for affinity chromatography
CN103429609A (zh) 2010-12-08 2013-12-04 安姆根有限公司 在氨基酸存在下的离子交换层析
TW201823460A (zh) 2012-05-29 2018-07-01 美商再生元醫藥公司 生產細胞株增強子
AU2015273049B2 (en) * 2014-06-13 2019-11-14 Lupin Limited Process for the purification of TNFR:Fc fusion protein
EP3236772A4 (en) * 2014-12-22 2018-10-17 Alexion Pharmaceuticals, Inc. Methods of purifying recombinant proteins
TW201702380A (zh) * 2015-02-27 2017-01-16 再生元醫藥公司 宿主細胞蛋白質修飾
AU2016261854B2 (en) 2015-05-12 2022-05-26 Regeneron Pharmaceuticals, Inc. Multimeric protein purity determination
CN106749660B (zh) * 2016-12-27 2020-02-14 嘉和生物药业有限公司 单克隆抗体下游纯化过程中有效去除宿主蛋白的方法
DK3668985T3 (da) 2017-08-17 2021-09-20 Just Evotec Biologics Inc Fremgangsmåde til rensning af glycosyleret protein fra værtscellegalectiner og andre urenheder
JP2022520793A (ja) * 2019-02-15 2022-04-01 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド 自動バイオ製造システム、施設、及びプロセス

Also Published As

Publication number Publication date
EP3668985B1 (en) 2021-06-16
JP2020531411A (ja) 2020-11-05
US20210163528A1 (en) 2021-06-03
JP7097433B2 (ja) 2022-07-07
WO2019036626A1 (en) 2019-02-21
ES2887046T3 (es) 2021-12-21
KR20200037396A (ko) 2020-04-08
US11358983B2 (en) 2022-06-14
CN111344410B (zh) 2023-09-15
KR102578087B1 (ko) 2023-09-18
EP3668985A1 (en) 2020-06-24
CA3067735A1 (en) 2019-02-21
DK3668985T3 (da) 2021-09-20
CN111344410A (zh) 2020-06-26

Similar Documents

Publication Publication Date Title
MY199833A (en) Method of purifying glycosylated protein from host cell galectins and other contaminants
MX381995B (es) Métodos para la producción de polipéptidos procesados proteolíticamente.
MX2020003798A (es) Metodos para la purificacion de arilsulfatasa a.
EA201492177A1 (ru) Очистка идуронат-2-сульфатазы
EP3543335A3 (en) Soluble intein fusion proteins and methods for purifying biomolecules
WO2016022377A3 (en) Methods for modulating the glycosylation profile of recombinant proteins using dissolved oxygen
MY180732A (en) Protein manufacture
CA3260163A1 (en) Method of purifying albumin-fusion proteins
PH12020550099A1 (en) Fusion protein comprising an fgf-18 moiety
PH12017502259A1 (en) Protease-resistant lipidated glp-1 analogs
IL304571A (en) Methods for the purification of recombinant proteins
EA201990052A1 (ru) ПОЛУЧЕНИЕ ПРОИЗВОДНЫХ ФАКТОРА Ха
PH12016500060A1 (en) Expanded bed absorption methods for isolation of basic milk proteins including lactoferrin
NZ744289A (en) Composition containing amino acids
MY184085A (en) Method of protein manufacture
IL307770A (en) Engineering B-cells based on protein production for the treatment of serious diseases
EP4118093A4 (en) METHOD FOR PURIFYING A FC FUSION PROTEIN
MY168902A (en) Glycosylated polypeptide and pharmaceutical composition containing same
IL284402A (en) A process for the production, isolation and purification of modified recombinant proteins
MX2019011343A (es) Un procedimiento para la purificacion de fragmentos de anticuerpos recombinantes.
GB201906595D0 (en) Method for purification of plasma proteins
IL308329A (en) A process for the production of recombinant proteins
IL256706B (en) A method for making recombinant proteins
IL256707B (en) A method for the production of recombinant proteins
MX2015000661A (es) Procesos de obtencion de quitina a partir de desechos de jaiba.