CA3067735A1 - Method of purifying glycosylated protein from host cell galectins and other contaminants - Google Patents

Method of purifying glycosylated protein from host cell galectins and other contaminants Download PDF

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Publication number
CA3067735A1
CA3067735A1 CA3067735A CA3067735A CA3067735A1 CA 3067735 A1 CA3067735 A1 CA 3067735A1 CA 3067735 A CA3067735 A CA 3067735A CA 3067735 A CA3067735 A CA 3067735A CA 3067735 A1 CA3067735 A1 CA 3067735A1
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CA
Canada
Prior art keywords
protein
galectin
buffer
matrix
poi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3067735A
Other languages
English (en)
French (fr)
Inventor
Lisa A. Connell-Crowley
Megan J. McClure
Ronald O. Gillespie
Original Assignee
Just Evotec Biologics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Just Evotec Biologics Inc filed Critical Just Evotec Biologics Inc
Publication of CA3067735A1 publication Critical patent/CA3067735A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CA3067735A 2017-08-17 2018-08-17 Method of purifying glycosylated protein from host cell galectins and other contaminants Pending CA3067735A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762546558P 2017-08-17 2017-08-17
US62/546,558 2017-08-17
PCT/US2018/046919 WO2019036626A1 (en) 2017-08-17 2018-08-17 PROCESS FOR PURIFYING GLYCOSYLATED PROTEIN FROM GALECTINES AND OTHER HOST CELL CONTAMINANTS

Publications (1)

Publication Number Publication Date
CA3067735A1 true CA3067735A1 (en) 2019-02-21

Family

ID=63684424

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3067735A Pending CA3067735A1 (en) 2017-08-17 2018-08-17 Method of purifying glycosylated protein from host cell galectins and other contaminants

Country Status (10)

Country Link
US (1) US11358983B2 (https=)
EP (1) EP3668985B1 (https=)
JP (1) JP7097433B2 (https=)
KR (1) KR102578087B1 (https=)
CN (1) CN111344410B (https=)
CA (1) CA3067735A1 (https=)
DK (1) DK3668985T3 (https=)
ES (1) ES2887046T3 (https=)
MY (1) MY199833A (https=)
WO (1) WO2019036626A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3668985T3 (da) 2017-08-17 2021-09-20 Just Evotec Biologics Inc Fremgangsmåde til rensning af glycosyleret protein fra værtscellegalectiner og andre urenheder
US11180540B2 (en) * 2019-12-06 2021-11-23 Regeneron Pharmaceuticals, Inc. Anti-VEGF protein compositions and methods for producing the same
JP2023525034A (ja) 2020-05-08 2023-06-14 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ｖｅｇｆトラップおよびミニトラップならびに眼障害およびがんの治療方法
WO2023192926A2 (en) * 2022-03-30 2023-10-05 Ionis Pharmaceuticals, Inc. Methods for separating certain oligonucleotide compounds
TW202502843A (zh) * 2023-03-17 2025-01-16 日商丸善石油化學股份有限公司 聚合物之製造方法

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Also Published As

Publication number Publication date
EP3668985B1 (en) 2021-06-16
JP2020531411A (ja) 2020-11-05
US20210163528A1 (en) 2021-06-03
JP7097433B2 (ja) 2022-07-07
WO2019036626A1 (en) 2019-02-21
ES2887046T3 (es) 2021-12-21
KR20200037396A (ko) 2020-04-08
US11358983B2 (en) 2022-06-14
CN111344410B (zh) 2023-09-15
MY199833A (en) 2023-11-24
KR102578087B1 (ko) 2023-09-18
EP3668985A1 (en) 2020-06-24
DK3668985T3 (da) 2021-09-20
CN111344410A (zh) 2020-06-26

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