CN111296200A - Method for cultivating insect-resistant transgenic millet - Google Patents
Method for cultivating insect-resistant transgenic millet Download PDFInfo
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- CN111296200A CN111296200A CN202010142588.XA CN202010142588A CN111296200A CN 111296200 A CN111296200 A CN 111296200A CN 202010142588 A CN202010142588 A CN 202010142588A CN 111296200 A CN111296200 A CN 111296200A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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Abstract
The embodiment of the invention discloses a method for cultivating insect-resistant transgenic millet, and particularly relates to the technical field of insect-resistant transgenic millet, which comprises the following steps: the method comprises the following steps: selecting a plurality of insect-resistant transgenic millet seeds, soaking the insect-resistant transgenic millet seeds in clear water for a certain time, and immediately taking out the shriveled shells floating on the water surface. By adopting a liquid-dripping fertilization method, during fertilization, the fertilizer is dissolved in clear water, the fertilizer is uniformly stirred and then introduced into a watering pipeline and is conveyed to the root of each seedling through the pipeline, so that the seedlings of the insect-resistant transgenic millet can quickly absorb the fertilizer, meanwhile, the quantity-controlled fertilization can be better continued on each seedling, the situations that the fertilizer of some seedlings is insufficient and the fertilizer of some seedlings is excessive are avoided, the development condition of the insect-resistant transgenic millet seedlings is increased, and specific data during cultivation of the insect-resistant transgenic millet seedlings can be recorded, so that people can conveniently further research the insect-resistant transgenic millet.
Description
Technical Field
The embodiment of the invention relates to the technical field of insect-resistant transgenic millet, and in particular relates to a cultivation method of insect-resistant transgenic millet.
Background
The research of transgenic biotechnology is mostly distributed in the fields of insect-resistant genetic engineering, salt-resistant genetic engineering, disease-resistant genetic engineering, stress-resistant genetic engineering, quality improvement genetic engineering, development control genetic engineering and the like. For example, Bt gene is introduced into cotton, corn, rice, tobacco and potato in pest-resistant gene engineering to kill pests.
The prior art has the following defects: in the existing process of cultivating the insect-resistant transgenic millet, the varieties of the insect-resistant transgenic millet to be fertilized are more, and a wide throwing mode is adopted in the fertilizing process, so that the conditions that some seedlings are insufficient in fertilizer and some seedlings are too much in fertilizer are easily caused, and the development condition of the insect-resistant transgenic millet is influenced.
Disclosure of Invention
Therefore, the embodiment of the invention provides a cultivation method of insect-resistant transgenic millet, which adopts a dropping liquid type fertilization method, wherein during fertilization, fertilizer is dissolved in clear water, uniformly stirred and then introduced into a watering pipeline, and conveyed to the root of each seedling through the pipeline, so that the seedlings of the insect-resistant transgenic millet can quickly absorb the fertilizer, meanwhile, the fertilizer can be continuously applied to each seedling in a controlled amount, the situations that the fertilizer of some seedlings is insufficient and the fertilizer of some seedlings is excessive are avoided, the development condition of the insect-resistant transgenic millet seedlings is increased, specific data during cultivation of the insect-resistant transgenic millet seedlings can be recorded, and further research on the insect-resistant transgenic millet is facilitated for people.
In order to achieve the above object, the embodiments of the present invention provide the following technical solutions: a cultivation method of insect-resistant transgenic millet comprises the following steps:
the method comprises the following steps: selecting a plurality of insect-resistant transgenic millet seeds, soaking the insect-resistant transgenic millet seeds in clear water for a certain time, and immediately taking out the shriveled shells floating on the water surface;
step two: taking out the soaked transgenic millet seeds from clear water, and then placing the seeds in a shade place for air drying to reduce the water in the transgenic millet seeds;
step three: spreading the air-dried transgenic millet seeds into a seedbed of a constant-temperature greenhouse, then pouring fertile black soil into the seedbed, covering the transgenic millet seeds, and then uniformly pouring nutrient solution into the seedbed;
step four: establishing a clear water conveying pipeline, arranging a plurality of through holes on the pipeline, wherein each through hole corresponds to the position of the transgenic millet seed, conveying clear water in the pipeline at regular time, and carrying out drip irrigation on the transgenic millet seed;
step five: after the seeds grow into small seedlings, mixing the fertilizer and clear water according to a certain proportion, then pouring the mixture into a stirrer, fully stirring the mixture until the fertilizer is completely dissolved in the clear water, and then introducing the solution into a conveying pipeline to fertilize the small seedlings;
step six: fertilizing and watering the seedlings according to a period, manually observing the growth condition of the transgenic millet seedlings every day, and recording the growth condition in a computer document;
step seven: after the transgenic millet is mature, introducing pests into a seedbed, waiting for a period of time, and observing the condition of the transgenic millet;
step eight: removing the transgenic millet seedlings without damage, taking down ears on the seedlings, cultivating the seedlings again, transplanting the seedlings into a field, and planting the seedlings in a large area;
step nine: and (4) cultivating the seedlings by adopting the methods of the fourth step, the fifth step and the sixth step, and harvesting after the transgenic millet is mature to obtain the insect-resistant transgenic millet.
Further, the soaking time of the fresh water for resisting the insect transgenic millet seeds in the first step is 3-5 h, and the soaking temperature of the fresh water in the first step is 30-40 ℃.
Further, the air drying time of the insect-resistant transgenic millet seeds in the second step is 4-6 h, and the tax content of the insect-resistant transgenic millet seeds after air drying in the second step is 10-20%.
Further, the temperature in the constant-temperature greenhouse in the third step is 30-40 ℃, and the area of the seedbed in the third step is 50m3-80m3The nutrient solution in the third step is specifically a calcium superphosphate solution.
Further, the conveying pipelines in the fourth step are made of stainless steel, the conveying pipelines in the fourth step are connected through threads, and the drip irrigation rate of the transgenic millet seeds in the fourth step is 20cm3/min-50cm3/min。
Further, the ratio of the fertilizer to clear water in the fifth step is 1.5:100, the fertilizer in the fifth step is specifically a potassium fertilizer and a phosphate fertilizer, and the stirring time of the stirrer in the fifth step is 30-50 min.
Further, the watering period in the sixth step is 2 times per day, and the fertilizing period in the sixth step is 7-10 days.
Further, the number of the introduced pests in the seventh step is 200-300, and the waiting time for introducing the pests in the seventh step is 5-7 days.
Further, the distance between the seedlings transplanted in the step eight in the field is 30cm-40 cm.
The embodiment of the invention has the following advantages:
by adopting a liquid-dripping fertilization method, during fertilization, the fertilizer is dissolved in clear water, the fertilizer is uniformly stirred and then introduced into a watering pipeline and is conveyed to the root of each seedling through the pipeline, so that the seedlings of the insect-resistant transgenic millet can quickly absorb the fertilizer, meanwhile, the quantity-controlled fertilization can be better continued on each seedling, the situations that the fertilizer of some seedlings is insufficient and the fertilizer of some seedlings is excessive are avoided, the development condition of the insect-resistant transgenic millet seedlings is increased, and specific data during cultivation of the insect-resistant transgenic millet seedlings can be recorded, so that people can conveniently further research the insect-resistant transgenic millet.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the method for cultivating the insect-resistant transgenic millet of the embodiment comprises the following steps:
the method comprises the following steps: selecting a plurality of insect-resistant transgenic millet seeds, soaking the insect-resistant transgenic millet seeds in clear water for a certain time, and immediately taking out the shriveled shells floating on the water surface;
step two: taking out the soaked transgenic millet seeds from clear water, and then placing the seeds in a shade place for air drying to reduce the water in the transgenic millet seeds;
step three: spreading the air-dried transgenic millet seeds into a seedbed of a constant-temperature greenhouse, then pouring fertile black soil into the seedbed, covering the transgenic millet seeds, and then uniformly pouring nutrient solution into the seedbed;
step four: establishing a clear water conveying pipeline, arranging a plurality of through holes on the pipeline, wherein each through hole corresponds to the position of the transgenic millet seed, conveying clear water in the pipeline at regular time, and carrying out drip irrigation on the transgenic millet seed;
step five: after the seeds grow into small seedlings, mixing the fertilizer and clear water according to a certain proportion, then pouring the mixture into a stirrer, fully stirring the mixture until the fertilizer is completely dissolved in the clear water, and then introducing the solution into a conveying pipeline to fertilize the small seedlings;
step six: fertilizing and watering the seedlings according to a period, manually observing the growth condition of the transgenic millet seedlings every day, and recording the growth condition in a computer document;
step seven: after the transgenic millet is mature, introducing pests into a seedbed, waiting for a period of time, and observing the condition of the transgenic millet;
step eight: removing the transgenic millet seedlings without damage, taking down ears on the seedlings, cultivating the seedlings again, transplanting the seedlings into a field, and planting the seedlings in a large area;
step nine: and (4) cultivating the seedlings by adopting the methods of the fourth step, the fifth step and the sixth step, and harvesting after the transgenic millet is mature to obtain the insect-resistant transgenic millet.
Further, the soaking time of the fresh water for resisting the insect transgenic millet seeds in the step one is 3 hours, and the soaking temperature of the fresh water in the step one is 30 ℃.
Further, the air drying time of the insect-resistant transgenic millet seeds in the second step is 4 hours, and the tax content of the insect-resistant transgenic millet seeds after the air drying in the second step is 10%.
Further, the temperature in the constant temperature greenhouse in the third step is 30 ℃, and the area of the seedbed in the third step is 50m3The nutrient solution in the third step is specifically a calcium superphosphate solution.
Further, the conveying pipelines in the fourth step are made of stainless steel, the conveying pipelines in the fourth step are connected through threads, and the drip irrigation rate of the transgenic millet seeds in the fourth step is 20cm3/min。
Further, the ratio of the fertilizer to clear water in the fifth step is 1.5:100, the fertilizer in the fifth step is specifically a potassium fertilizer and a phosphate fertilizer, and the stirring time of the stirrer in the fifth step is 30 min.
Further, the watering period in the sixth step is 2 times per day, and the fertilizing period in the sixth step is 7 days.
Further, the number of introduced pests in said seventh step is 200, and the waiting time for introduction of pests in said seventh step is 5 days.
Further, the distance between the seedlings transplanted in the step eight in the field is 30 cm.
Example 2:
a cultivation method of insect-resistant transgenic millet comprises the following steps:
the method comprises the following steps: selecting a plurality of insect-resistant transgenic millet seeds, soaking the insect-resistant transgenic millet seeds in clear water for a certain time, and immediately taking out the shriveled shells floating on the water surface;
step two: taking out the soaked transgenic millet seeds from clear water, and then placing the seeds in a shade place for air drying to reduce the water in the transgenic millet seeds;
step three: spreading the air-dried transgenic millet seeds into a seedbed of a constant-temperature greenhouse, then pouring fertile black soil into the seedbed, covering the transgenic millet seeds, and then uniformly pouring nutrient solution into the seedbed;
step four: establishing a clear water conveying pipeline, arranging a plurality of through holes on the pipeline, wherein each through hole corresponds to the position of the transgenic millet seed, conveying clear water in the pipeline at regular time, and carrying out drip irrigation on the transgenic millet seed;
step five: after the seeds grow into small seedlings, mixing the fertilizer and clear water according to a certain proportion, then pouring the mixture into a stirrer, fully stirring the mixture until the fertilizer is completely dissolved in the clear water, and then introducing the solution into a conveying pipeline to fertilize the small seedlings;
step six: fertilizing and watering the seedlings according to a period, manually observing the growth condition of the transgenic millet seedlings every day, and recording the growth condition in a computer document;
step seven: after the transgenic millet is mature, introducing pests into a seedbed, waiting for a period of time, and observing the condition of the transgenic millet;
step eight: removing the transgenic millet seedlings without damage, taking down ears on the seedlings, cultivating the seedlings again, transplanting the seedlings into a field, and planting the seedlings in a large area;
step nine: and (4) cultivating the seedlings by adopting the methods of the fourth step, the fifth step and the sixth step, and harvesting after the transgenic millet is mature to obtain the insect-resistant transgenic millet.
Further, the soaking time of the fresh water for resisting the insect transgenic millet seeds in the first step is 3-5 h, and the soaking temperature of the fresh water in the first step is 40 ℃.
Further, the air drying time of the insect-resistant transgenic millet seeds in the second step is 6 hours, and the tax content of the insect-resistant transgenic millet seeds after the air drying in the second step is 20%.
Further, the temperature in the constant temperature greenhouse in the third step is 40 ℃, and the area of the seedbed in the third step is 80m3The nutrient solution in the third step is specifically a calcium superphosphate solution.
Furthermore, the conveying pipelines in the fourth step are made of stainless steel, the conveying pipelines in the fourth step are connected through threads, and the fourth step is used for carrying out gene conversionThe drip irrigation rate of the millet seeds is 50cm3/min。
Further, the ratio of the fertilizer to clear water in the fifth step is 1.5:100, the fertilizer in the fifth step is specifically a potassium fertilizer and a phosphate fertilizer, and the stirring time of the stirrer in the fifth step is 50 min.
Further, the watering period in the sixth step is 2 times per day, and the fertilizing period in the sixth step is 10 days.
Further, the number of introduced pests in said seventh step is 300, and the waiting time for introducing pests in said seventh step is 7 days.
Further, the distance between the seedlings transplanted in the step eight in the field is 40 cm.
Example 3:
a cultivation method of insect-resistant transgenic millet comprises the following steps:
the method comprises the following steps: selecting a plurality of insect-resistant transgenic millet seeds, soaking the insect-resistant transgenic millet seeds in clear water for a certain time, and immediately taking out the shriveled shells floating on the water surface;
step two: taking out the soaked transgenic millet seeds from clear water, and then placing the seeds in a shade place for air drying to reduce the water in the transgenic millet seeds;
step three: spreading the air-dried transgenic millet seeds into a seedbed of a constant-temperature greenhouse, then pouring fertile black soil into the seedbed, covering the transgenic millet seeds, and then uniformly pouring nutrient solution into the seedbed;
step four: establishing a clear water conveying pipeline, arranging a plurality of through holes on the pipeline, wherein each through hole corresponds to the position of the transgenic millet seed, conveying clear water in the pipeline at regular time, and carrying out drip irrigation on the transgenic millet seed;
step five: after the seeds grow into small seedlings, mixing the fertilizer and clear water according to a certain proportion, then pouring the mixture into a stirrer, fully stirring the mixture until the fertilizer is completely dissolved in the clear water, and then introducing the solution into a conveying pipeline to fertilize the small seedlings;
step six: fertilizing and watering the seedlings according to a period, manually observing the growth condition of the transgenic millet seedlings every day, and recording the growth condition in a computer document;
step seven: after the transgenic millet is mature, introducing pests into a seedbed, waiting for a period of time, and observing the condition of the transgenic millet;
step eight: removing the transgenic millet seedlings without damage, taking down ears on the seedlings, cultivating the seedlings again, transplanting the seedlings into a field, and planting the seedlings in a large area;
step nine: and (4) cultivating the seedlings by adopting the methods of the fourth step, the fifth step and the sixth step, and harvesting after the transgenic millet is mature to obtain the insect-resistant transgenic millet.
Further, the soaking time of the fresh water for resisting the insect transgenic millet seeds in the step one is 4 hours, and the soaking temperature of the fresh water in the step one is 35 ℃.
Further, the air drying time of the insect-resistant transgenic millet seeds in the second step is 5 hours, and the tax content of the insect-resistant transgenic millet seeds after the air drying in the second step is 15%.
Further, the temperature in the constant temperature greenhouse in the third step is 35 ℃, and the area of the seedbed in the third step is 60m3The nutrient solution in the third step is specifically a calcium superphosphate solution.
Further, the conveying pipelines in the fourth step are made of stainless steel, the conveying pipelines in the fourth step are connected through threads, and the drip irrigation rate of the transgenic millet seeds in the fourth step is 30cm3/min。
Further, the ratio of the fertilizer to clear water in the fifth step is 1.5:100, the fertilizer in the fifth step is specifically a potassium fertilizer and a phosphate fertilizer, and the stirring time of the stirrer in the fifth step is 40 min.
Further, the watering period in the sixth step is 2 times per day, and the fertilizing period in the sixth step is 8 days.
Further, the number of introduced pests in said seventh step is 250, and the waiting time for introducing pests in said seventh step is 6 days.
Further, the distance between the seedlings transplanted in the step eight in the field is 30cm-40 cm.
Example 4:
the insect-resistant transgenic millet was cultured according to the methods of examples 1 to 3, and the data detected in examples 1 to 3 were compared to obtain the following data:
as can be seen from the above table, the cultivation method of transgenic millet in example 3 has high insect resistance, short production cycle, and high mass production.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (9)
1. A cultivation method of insect-resistant transgenic millet is characterized by comprising the following steps: the method comprises the following steps:
the method comprises the following steps: selecting a plurality of insect-resistant transgenic millet seeds, soaking the insect-resistant transgenic millet seeds in clear water for a certain time, and immediately taking out the shriveled shells floating on the water surface;
step two: taking out the soaked transgenic millet seeds from clear water, and then placing the seeds in a shade place for air drying to reduce the water in the transgenic millet seeds;
step three: spreading the air-dried transgenic millet seeds into a seedbed of a constant-temperature greenhouse, then pouring fertile black soil into the seedbed, covering the transgenic millet seeds, and then uniformly pouring nutrient solution into the seedbed;
step four: establishing a clear water conveying pipeline, arranging a plurality of through holes on the pipeline, wherein each through hole corresponds to the position of the transgenic millet seed, conveying clear water in the pipeline at regular time, and carrying out drip irrigation on the transgenic millet seed;
step five: after the seeds grow into small seedlings, mixing the fertilizer and clear water according to a certain proportion, then pouring the mixture into a stirrer, fully stirring the mixture until the fertilizer is completely dissolved in the clear water, and then introducing the solution into a conveying pipeline to fertilize the small seedlings;
step six: fertilizing and watering the seedlings according to a period, manually observing the growth condition of the transgenic millet seedlings every day, and recording the growth condition in a computer document;
step seven: after the transgenic millet is mature, introducing pests into a seedbed, waiting for a period of time, and observing the condition of the transgenic millet;
step eight: removing the transgenic millet seedlings without damage, taking down ears on the seedlings, cultivating the seedlings again, transplanting the seedlings into a field, and planting the seedlings in a large area;
step nine: and (4) cultivating the seedlings by adopting the methods of the fourth step, the fifth step and the sixth step, and harvesting after the transgenic millet is mature to obtain the insect-resistant transgenic millet.
2. The method for cultivating insect-resistant transgenic millet according to claim 1, wherein the method comprises the following steps: the soaking time of the fresh water to resist the insect transgenic millet seeds in the step one is 3-5 h, and the soaking temperature of the fresh water in the step one is 30-40 ℃.
3. The method for cultivating insect-resistant transgenic millet according to claim 1, wherein the method comprises the following steps: the air drying time of the insect-resistant transgenic millet seeds in the second step is 4-6 h, and the tax content of the insect-resistant transgenic millet seeds after air drying in the second step is 10-20%.
4. The method for cultivating insect-resistant transgenic millet according to claim 1, wherein the method comprises the following steps: the temperature in the constant temperature greenhouse in the third step is 30-40 ℃, and the area of the seedbed in the third step is 50m3-80m3The nutrient solution in the third step is specifically a calcium superphosphate solution.
5. The method for cultivating insect-resistant transgenic millet according to claim 1, wherein the method comprises the following steps: conveying pipelines in the fourth step are made of stainless steel, the conveying pipelines in the fourth step are connected through threads, and the drip irrigation rate of transgenic millet seeds in the fourth step is 20cm3/min-50cm3/min。
6. The method for cultivating insect-resistant transgenic millet according to claim 1, wherein the method comprises the following steps: the ratio of the fertilizer to the clear water in the fifth step is 1.5:100, the fertilizer in the fifth step is specifically a potassium fertilizer and a phosphate fertilizer, and the stirring time of the stirrer in the fifth step is 30-50 min.
7. The method for cultivating insect-resistant transgenic millet according to claim 1, wherein the method comprises the following steps: the watering period in the sixth step is 2 times per day, and the fertilizing period in the sixth step is 7-10 days.
8. The method for cultivating insect-resistant transgenic millet according to claim 1, wherein the method comprises the following steps: the number of the introduced pests in the step seven is 200-300, and the waiting time for introducing the pests in the step seven is 5-7 days.
9. The method for cultivating insect-resistant transgenic millet according to claim 1, wherein the method comprises the following steps: and the distance between the seedlings transplanted in the step eight in the field is 30-40 cm.
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| CN104099367A (en) * | 2013-04-15 | 2014-10-15 | 华中农业大学 | Method for culturing transgenic insect-resistant paddy rice |
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