CN108849338A - A kind of breeding method of pest-resistant transgenic rice - Google Patents
A kind of breeding method of pest-resistant transgenic rice Download PDFInfo
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- CN108849338A CN108849338A CN201810869308.8A CN201810869308A CN108849338A CN 108849338 A CN108849338 A CN 108849338A CN 201810869308 A CN201810869308 A CN 201810869308A CN 108849338 A CN108849338 A CN 108849338A
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- rice
- seed
- pest
- seed rice
- resistant transgenic
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a kind of breeding methods of pest-resistant transgenic rice, include the following steps：A, seed rice is chosen, 2% brine ice of concentration is contained with heavy caliber container, seed rice is uniformly removed and is sunk naturally in the cryosel water surface by it, the flat shell bubbled through the water column is pulled out immediately, then the full seed rice being sunken in water is allowed to impregnate 2-3h in brine ice, taking-up paves dries in the air ventilation in yin, drains seed rice surface moisture, is subject between seed rice and is not overlapped；B, nursery uniformly paves seed rice on burlap, and paving thickness 6-8mm makes burlap be immersed in nutrient solution surface, stirs once every 6h；C, seedbed is handled；D, greenhouse is built；E, rice cultivating；F, secondary cultivation；G, preferably breeding.Beneficial effect is：Pest-resistant transgenic rice is cultivated using the method for preferred breeding, secondary cultivation can be carried out to rice after transgenosis, preferably the higher seed rice of insect resistace in transgenic paddy rice out, and is not required to spray insecticide in cultivating process.
Description
Technical field
The present invention relates to Rice Cropping technical fields, more particularly to a kind of breeding method of pest-resistant transgenic rice.
Background technique
The research of change gene biology technology is distributed in Insect resistant gene engineer, salt resistant gene engineering, disease-resistant gene work mostly
The fields such as journey, adversity gene engineering, Quality Gene engineering, quality-improving genetic engineering, the genetic engineering of control development.It is such as pest-resistant
Bt channel genes cotton, corn, rice, tobacco, potatoes and other crops are killed pest by genetic engineering；Or glue protein enzyme is pressed down
Preparation gene transferred crop interferes pest digestion, and causes pest dead.Transgenic paddy rice refers to through transgenosis skill
Anti insect gene, disease-resistant gene of different cultivars rice or nearly edge species etc. is imported the water cultivated in certain rice genome by art
Rice varieties.The breeding method of existing pest-resistant transgenic rice is mostly disposable cultivation, effectively can not preferably be gone out therein anti-
The preferable rice seed rice of worm property.
Summary of the invention
The object of the invention is that providing a kind of breeding method of pest-resistant transgenic rice to solve the above-mentioned problems.
The present invention is achieved through the following technical solutions above-mentioned purpose：
A kind of breeding method of pest-resistant transgenic rice, includes the following steps：
A, seed rice choose, with heavy caliber container contain 2% brine ice of concentration, seed rice is uniformly removed in the cryosel water surface by its from
So sink, the flat shell bubbled through the water column is pulled out immediately, and the full seed rice being sunken in water is then allowed to impregnate 2-3h in brine ice, is taken out
It paves and dries in the air ventilation in yin, drain seed rice surface moisture, be subject between seed rice and be not overlapped；
B, nursery uniformly paves seed rice on burlap, and paving thickness 6-8mm makes burlap be immersed in nutrient solution surface,
It stirs once every 6h, and takes out seed rice daily and eluriated once with clear water, there is seed rice to grow gemma after 5-6 days, take out a gemma
Seed；
C, seedbed is handled, and selects suitable soil as the seedbed of rice cultivating, farming goes out to cultivate layer on seedbed, by potassium
Fertilizer, phosphate fertilizer, calcium superphosphate solution are sufficiently mixed with cultivation layer and are pre-processed；
D, greenhouse is built, and is built arch greenhouse and is created suitable condition quickening speed of emergence, wait be transplanted after the completion of emerging,
Sprinkling aliette solution carries out diseases prevention before transplanting, also to apply the nitrogen content that urea increases seedling root；
E, rice cultivating sprays water to the seedling medium on seedbed, so that its water content is reached saturation, and do not seep water outward,
The seed rice for growing gemma is uniformly removed again and is turned on the seedling medium in seedbed, removing the seed rice spacing broadcast is 20-25mm, uses plant ash
Seed rice gemma is covered, arched shed is then built on seedbed, it is long to 7-10mm high to rice shoot after 12-14 days, arched shed two is opened daily
End is aerated, then rice shoot is long to 3-4cm high after 15 days, removes arched shed, rice shoot is long to 5-6cm after 7 days, can cultivate rice；
F, with the obvious advantage every fringe sowing 35~55 of disease and insect resistance in rice are received 5500~6500 by secondary cultivation altogether
Grain seed；
G, preferably breeding, 40 plants of every cell, the plantation of 5 × 8 rows；Repeat the above steps, when the maturity period, first carry out cell it
Between insect resistace compare, carry out comparing between individual again in the good cell of insect resistace, choose the good single plant of variety character, carry out room
Spike length, spike weight, Spikelet density, setting percentage and exterior quality are investigated in interior species test, retain the preferable seed rice of quality；
In the present embodiment, environment temperature is 32-35 DEG C, wind speed 4-6m/s in the step a, carries out yin and dries in the air 6-8h.
In the present embodiment, the seed rice in the step b is placed on the environment that temperature is 30-34 DEG C, humidity is 42-48%
In.
In the present embodiment, the depth of the cultivation layer in the step c is 6-10cm, and the parts by weight of the potash fertilizer are 3-3.6,
The parts by weight of the phosphate fertilizer are 1.88-1.93, and the parts by weight of the calcium superphosphate solution are 120-133.
In the present embodiment, the good PVC plastic film of greenhouse surface bedding translucency in the step d.
In the present embodiment, the plant ash thickness in the step e is not less than 20mm, and control compound seedling medium water content is 40-
60%.
In the present embodiment, the average spike length of selection reservation is at 22 centimetres~25 centimetres in the step f, average spike weight 5.2
Gram~5.8 grams, Spikelet density 9.2/centimetre~9.8/centimetre between, setting percentage is in 95% or more, rice insect pest area
Rice no more than 5% carries out sowing.
In the present embodiment, reservation is averaged spike length at 22 centimetres~25 centimetres in the step g, and 5.2 grams of the spike weight that is averaged~
5.8 grams, Spikelet density 9.2/centimetre~9.8/centimetre between, setting percentage 95% or more single plant as seed rice, obtain
Target variety seed.
The beneficial effects of the present invention are：Pest-resistant transgenic rice is cultivated using the method for preferred breeding, it can
Secondary cultivation is carried out to rice after transgenosis, preferably the higher seed rice of insect resistace in transgenic paddy rice out, and in cultivating process
It is not required to spray insecticide.
Specific embodiment
Embodiment one：
A kind of breeding method of pest-resistant transgenic rice, includes the following steps：
A kind of breeding method of pest-resistant transgenic rice, includes the following steps：
A, seed rice choose, with heavy caliber container contain 2% brine ice of concentration, seed rice is uniformly removed in the cryosel water surface by its from
So sink, the flat shell bubbled through the water column is pulled out immediately, and the full seed rice being sunken in water is then allowed to impregnate 2-3h in brine ice, is taken out
It paves and dries in the air ventilation in yin, drain seed rice surface moisture, be subject between seed rice and be not overlapped；
B, nursery uniformly paves seed rice on burlap, and paving thickness 6-8mm makes burlap be immersed in nutrient solution surface,
It stirs once every 6h, and takes out seed rice daily and eluriated once with clear water, there is seed rice to grow gemma after 5-6 days, take out a gemma
Seed；
C, seedbed is handled, and selects suitable soil as the seedbed of rice cultivating, farming goes out to cultivate layer on seedbed, by potassium
Fertilizer, phosphate fertilizer, calcium superphosphate solution are sufficiently mixed with cultivation layer and are pre-processed；
D, greenhouse is built, and is built arch greenhouse and is created suitable condition quickening speed of emergence, wait be transplanted after the completion of emerging,
Sprinkling aliette solution carries out diseases prevention before transplanting, also to apply the nitrogen content that urea increases seedling root；
E, rice cultivating sprays water to the seedling medium on seedbed, so that its water content is reached saturation, and do not seep water outward,
The seed rice for growing gemma is uniformly removed again and is turned on the seedling medium in seedbed, removing the seed rice spacing broadcast is 20-25mm, uses plant ash
Seed rice gemma is covered, arched shed is then built on seedbed, it is long to 7-10mm high to rice shoot after 12-14 days, arched shed two is opened daily
End is aerated, then rice shoot is long to 3-4cm high after 15 days, removes arched shed, rice shoot is long to 5-6cm after 7 days, can cultivate rice；
F, with the obvious advantage every fringe sowing 35~55 of disease and insect resistance in rice are received 5500~6500 by secondary cultivation altogether
Grain seed；
G, preferably breeding, 40 plants of every cell, the plantation of 5 × 8 rows；Repeat the above steps, when the maturity period, first carry out cell it
Between insect resistace compare, carry out comparing between individual again in the good cell of insect resistace, choose the good single plant of variety character, carry out room
Spike length, spike weight, Spikelet density, setting percentage and exterior quality are investigated in interior species test, retain the preferable seed rice of quality；
In the present embodiment, environment temperature is 32-35 DEG C, wind speed 4-6m/s in the step a, carries out yin and dries in the air 6-8h.
In the present embodiment, the seed rice in the step b is placed on the environment that temperature is 30-34 DEG C, humidity is 42-48%
In.
In the present embodiment, the depth of the cultivation layer in the step c is 6-10cm, and the parts by weight of the potash fertilizer are 3-3.6,
The parts by weight of the phosphate fertilizer are 1.88-1.93, and the parts by weight of the calcium superphosphate solution are 120-133.
In the present embodiment, the good PVC plastic film of greenhouse surface bedding translucency in the step d.
In the present embodiment, the plant ash thickness in the step e is not less than 20mm, and control compound seedling medium water content is 40-
60%.
In the present embodiment, the average spike length of selection reservation is at 22 centimetres~25 centimetres in the step f, average spike weight 5.2
Gram~5.8 grams, Spikelet density 9.2/centimetre~9.8/centimetre between, setting percentage is in 95% or more, rice insect pest area
Rice no more than 5% carries out sowing.
In the present embodiment, reservation is averaged spike length at 22 centimetres~25 centimetres in the step g, and 5.2 grams of the spike weight that is averaged~
5.8 grams, Spikelet density 9.2/centimetre~9.8/centimetre between, setting percentage 95% or more single plant as seed rice, obtain
Target variety seed.
Embodiment two：
A kind of breeding method of pest-resistant transgenic rice, includes the following steps：
A, seed rice choose, with heavy caliber container contain 2% brine ice of concentration, seed rice is uniformly removed in the cryosel water surface by its from
So sink, the flat shell bubbled through the water column is pulled out immediately, and the full seed rice being sunken in water is then allowed to impregnate 2-3h in brine ice, is taken out
It paves and dries in the air ventilation in yin, drain seed rice surface moisture, be subject between seed rice and be not overlapped；
B, nursery uniformly paves seed rice on burlap, and paving thickness 6-8mm makes burlap be immersed in nutrient solution surface,
It stirs once every 6h, and takes out seed rice daily and eluriated once with clear water, there is seed rice to grow gemma after 5-6 days, take out a gemma
Seed；
C, seedbed is handled, and selects suitable soil as the seedbed of rice cultivating, farming goes out to cultivate layer on seedbed, by potassium
Fertilizer, phosphate fertilizer, calcium superphosphate solution are sufficiently mixed with cultivation layer and are pre-processed；
D, greenhouse is built, and is built arch greenhouse and is created suitable condition quickening speed of emergence, wait be transplanted after the completion of emerging,
Sprinkling aliette solution carries out diseases prevention before transplanting, also to apply the nitrogen content that urea increases seedling root；
E, rice cultivating sprays water to the seedling medium on seedbed, so that its water content is reached saturation, and do not seep water outward,
The seed rice for growing gemma is uniformly removed again and is turned on the seedling medium in seedbed, removing the seed rice spacing broadcast is 20-25mm, uses plant ash
Seed rice gemma is covered, arched shed is then built on seedbed, it is long to 7-10mm high to rice shoot after 12-14 days, arched shed two is opened daily
End is aerated, then rice shoot is long to 3-4cm high after 15 days, removes arched shed, rice shoot is long to 5-6cm after 7 days, can cultivate rice；
F, with the obvious advantage every fringe sowing 35~55 of disease and insect resistance in rice are received 5500~6500 by secondary cultivation altogether
Grain seed；
G, preferably breeding, 40 plants of every cell, the plantation of 5 × 8 rows；Repeat the above steps, when the maturity period, first carry out cell it
Between insect resistace compare, carry out comparing between individual again in the good cell of insect resistace, choose the good single plant of variety character, carry out room
Spike length, spike weight, Spikelet density, setting percentage and exterior quality are investigated in interior species test, retain the preferable seed rice of quality.
In the present embodiment, environment temperature is 38-45 DEG C, wind speed 4-6m/s in the step a, carries out yin and dries in the air 12h.
In the present embodiment, the seed rice in the step b is placed on the environment that temperature is 35-40 DEG C, humidity is 32-38%
In.
In the present embodiment, the depth of the cultivation layer in the step c is 6-10cm, and the parts by weight of the potash fertilizer are 3-3.6,
The parts by weight of the phosphate fertilizer are 1.88-1.93, and the parts by weight of the calcium superphosphate solution are 120-133.
In the present embodiment, the good PVC plastic film of greenhouse surface bedding translucency in the step d.
In the present embodiment, the plant ash thickness in the step e is not less than 20mm, and control compound seedling medium water content is 40-
60%.
In the present embodiment, the average spike length of selection reservation is at 17 centimetres~20 centimetres in the step f, average spike weight 5.2
Gram~5.8 grams, Spikelet density 9.2/centimetre~9.8/centimetre between, setting percentage is in 95% or more, rice insect pest area
Rice no more than 5% carries out sowing.
In the present embodiment, reservation is averaged spike length at 17 centimetres~20 centimetres in the step g, and 5.2 grams of the spike weight that is averaged~
5.8 grams, Spikelet density 9.2/centimetre~9.8/centimetre between, setting percentage 95% or more single plant as seed rice, obtain
Target variety seed
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe originals of the invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appending claims and equivalents circle
It is fixed.
Claims (8)
1. a kind of breeding method of pest-resistant transgenic rice, it is characterised in that：Include the following steps：
A, seed rice is chosen, and contains 2% brine ice of concentration with heavy caliber container, seed rice is uniformly removed in the cryosel water surface by under its nature
Heavy, the flat shell bubbled through the water column is pulled out immediately, then allows the full seed rice being sunken in water to impregnate 2-3h in brine ice, taking-up paves
It dries in the air ventilation in yin, drains seed rice surface moisture, be subject between seed rice and be not overlapped；
B, nursery uniformly paves seed rice on burlap, and paving thickness 6-8mm makes burlap be immersed in nutrient solution surface, every
6h stirs once, and takes out seed rice daily and eluriated once with clear water, has seed rice to grow gemma after 5-6 days, takes out the kind of a gemma
Son；
C, seedbed is handled, and selects suitable soil as the seedbed of rice cultivating, and farming goes out cultivation layer on seedbed, by potash fertilizer,
Phosphate fertilizer, calcium superphosphate solution are sufficiently mixed with cultivation layer and are pre-processed；
D, greenhouse is built, and is built arch greenhouse and is created suitable condition quickening speed of emergence, wait be transplanted after the completion of emerging, transplanting
Preceding sprinkling aliette solution carries out diseases prevention, also to apply the nitrogen content that urea increases seedling root；
E, rice cultivating sprays water to the seedling medium on seedbed, so that its water content is reached saturation, and do not seep water outward, then will
The seed rice for growing gemma, which is uniformly removed, to be turned on the seedling medium in seedbed, and removing the seed rice spacing broadcast is 20-25mm, is covered with plant ash
Then seed rice gemma builds arched shed on seedbed, long to 7-10mm high to rice shoot after 12-14 days, daily opening arched shed both ends into
Row ventilation, then rice shoot is long to 3-4cm high after 15 days, removes arched shed, rice shoot is long to 5-6cm after 7 days, can cultivate rice；
F, with the obvious advantage every fringe sowing 35~55 of disease and insect resistance in rice are received 5500~6500 kinds by secondary cultivation altogether
Son；
G, preferably breeding, 40 plants of every cell, the plantation of 5 × 8 rows；It repeats the above steps, when the maturity period, first carries out between cell
Insect resistace compares, and carries out comparing between individual again in the good cell of insect resistace, chooses the good single plant of variety character, carries out interior and examines
Kind, spike length, spike weight, Spikelet density, setting percentage and exterior quality are investigated, the preferable seed rice of quality is retained.
2. a kind of breeding method of pest-resistant transgenic rice according to claim 1, it is characterised in that：In the step a
Environment temperature is 32-35 DEG C, wind speed 4-6m/s, carries out yin and dries in the air 6-8h.
3. a kind of breeding method of pest-resistant transgenic rice according to claim 1, it is characterised in that：In the step b
Seed rice be placed on that temperature is 30-34 DEG C, humidity is in the environment of 42-48%.
4. a kind of breeding method of pest-resistant transgenic rice according to claim 1, it is characterised in that：In the step c
The depth of cultivation layer be 6-10cm, the parts by weight of the potash fertilizer are 3-3.6, and the parts by weight of the phosphate fertilizer are 1.88-1.93, institute
The parts by weight for stating calcium superphosphate solution are 120-133.
5. a kind of breeding method of pest-resistant transgenic rice according to claim 1, it is characterised in that：In the step d
The good PVC plastic film of greenhouse surface bedding translucency.
6. a kind of breeding method of pest-resistant transgenic rice according to claim 1, it is characterised in that：In the step e
Plant ash thickness be not less than 20mm, control compound seedling medium water content be 40-60%.
7. a kind of breeding method of pest-resistant transgenic rice according to claim 1, it is characterised in that：In the step f
Selection retains average spike length at 22 centimetres~25 centimetres, and average 5.2 grams~5.8 grams of spike weight, Spikelet density is in 9.2/centimetre
Between~9.8/centimetre, setting percentage carries out sowing in rice of 95% or more, the rice insect pest area no more than 5%.
8. a kind of breeding method of pest-resistant transgenic rice according to claim 1, it is characterised in that：In the step g
Retain average spike length at 22 centimetres~25 centimetres, average 5.2 grams~5.8 grams of spike weight, Spikelet density is in 9.2/centimetre~9.8
Grain/centimetre between, setting percentage 95% or more single plant as seed rice, obtain target variety seed.
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| CN201810869308.8A CN108849338A (en) | 2018-08-02 | 2018-08-02 | A kind of breeding method of pest-resistant transgenic rice |
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| CN201810869308.8A CN108849338A (en) | 2018-08-02 | 2018-08-02 | A kind of breeding method of pest-resistant transgenic rice |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296200A (en) * | 2020-03-04 | 2020-06-19 | 黔东南苗族侗族自治州农业科学院 | Method for cultivating insect-resistant transgenic millet |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105104043A (en) * | 2015-08-17 | 2015-12-02 | 太仓市雅丰农场专业合作社 | Cultivating method of rice |
| CN106665227A (en) * | 2016-12-14 | 2017-05-17 | 五河县茂源水蛭生态养殖专业合作社 | Method for cultivating high-yield paddy rice in high tillering |
| CN107750939A (en) * | 2017-09-26 | 2018-03-06 | 眉山市东坡区德祥蔬菜专业合作社 | A kind of rice cultivating method |
-
2018
- 2018-08-02 CN CN201810869308.8A patent/CN108849338A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105104043A (en) * | 2015-08-17 | 2015-12-02 | 太仓市雅丰农场专业合作社 | Cultivating method of rice |
| CN106665227A (en) * | 2016-12-14 | 2017-05-17 | 五河县茂源水蛭生态养殖专业合作社 | Method for cultivating high-yield paddy rice in high tillering |
| CN107750939A (en) * | 2017-09-26 | 2018-03-06 | 眉山市东坡区德祥蔬菜专业合作社 | A kind of rice cultivating method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296200A (en) * | 2020-03-04 | 2020-06-19 | 黔东南苗族侗族自治州农业科学院 | Method for cultivating insect-resistant transgenic millet |
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Application publication date: 20181123 |