CN111289425B - 基于荧光标记流式单分子计数的蛋白质含量计量基准方法 - Google Patents
基于荧光标记流式单分子计数的蛋白质含量计量基准方法 Download PDFInfo
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- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
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- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010170831.9A CN111289425B (zh) | 2020-03-12 | 2020-03-12 | 基于荧光标记流式单分子计数的蛋白质含量计量基准方法 |
| PCT/CN2021/080008 WO2021180129A1 (fr) | 2020-03-12 | 2021-03-10 | Procédé de référence de mesure de teneur en protéine basé sur un comptage de molécule unique de type à flux par marquage par fluorescence |
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| CN202010170831.9A CN111289425B (zh) | 2020-03-12 | 2020-03-12 | 基于荧光标记流式单分子计数的蛋白质含量计量基准方法 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111289425B (zh) * | 2020-03-12 | 2021-03-30 | 中国计量科学研究院 | 基于荧光标记流式单分子计数的蛋白质含量计量基准方法 |
| CN111724857B (zh) * | 2020-07-07 | 2021-06-15 | 中国计量科学研究院 | 一种免疫分析中蛋白质溯源有效性及互换性评价方法 |
| CN112457969B (zh) * | 2020-11-30 | 2022-05-27 | 中国计量科学研究院 | 基于微流控芯片的单分子计数生物大分子计量方法 |
| CN115575358B (zh) * | 2021-06-21 | 2024-05-24 | 北京大学 | 一种对单分子荧光共振能量转移信号进行分析的方法 |
| CN115980336B (zh) * | 2022-12-26 | 2024-03-15 | 南京市计量监督检测院 | 一种白介素-2标准品及其制备方法 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105378450A (zh) * | 2013-02-18 | 2016-03-02 | 赛拉诺斯股份有限公司 | 用于多元分析的系统和方法 |
| EP3418738A1 (fr) * | 2017-06-23 | 2018-12-26 | Klinikum rechts der Isar der Technischen Universität München | Un rapport de cellules immunitaires comme indicateur pronostique de succès thérapeutique dans le immunothérapie spécifique aux allergènes |
| CN109475863A (zh) * | 2016-06-30 | 2019-03-15 | 卢米瑞德思英国有限公司 | 流体控制 |
| US20190277845A1 (en) * | 2013-03-15 | 2019-09-12 | Senomyx, Inc. | Screening assays to identify compounds which modulate t1r associated taste modalities which eliminate false positives |
| CN110249082A (zh) * | 2016-12-01 | 2019-09-17 | 点燃生物科学有限公司 | 测定蛋白质的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835110B2 (en) * | 2008-11-04 | 2014-09-16 | The Johns Hopkins University | DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy |
| WO2012144528A1 (fr) * | 2011-04-18 | 2012-10-26 | オリンパス株式会社 | Procédé de détermination quantitative de particules cibles, dispositif d'analyse photométrique et programme informatique d'analyse photométrique |
| US20190369106A1 (en) * | 2018-05-29 | 2019-12-05 | Board Of Regents, The University Of Texas System | Flow proteometric methods for digital quantification and binding analysis |
| CN109142756B (zh) * | 2018-10-29 | 2022-02-08 | 领航基因科技（杭州）有限公司 | 一种单分子蛋白的检测方法 |
| CN111289425B (zh) * | 2020-03-12 | 2021-03-30 | 中国计量科学研究院 | 基于荧光标记流式单分子计数的蛋白质含量计量基准方法 |
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2020
- 2020-03-12 CN CN202010170831.9A patent/CN111289425B/zh active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105378450A (zh) * | 2013-02-18 | 2016-03-02 | 赛拉诺斯股份有限公司 | 用于多元分析的系统和方法 |
| US20190277845A1 (en) * | 2013-03-15 | 2019-09-12 | Senomyx, Inc. | Screening assays to identify compounds which modulate t1r associated taste modalities which eliminate false positives |
| CN109475863A (zh) * | 2016-06-30 | 2019-03-15 | 卢米瑞德思英国有限公司 | 流体控制 |
| CN110249082A (zh) * | 2016-12-01 | 2019-09-17 | 点燃生物科学有限公司 | 测定蛋白质的方法 |
| EP3418738A1 (fr) * | 2017-06-23 | 2018-12-26 | Klinikum rechts der Isar der Technischen Universität München | Un rapport de cellules immunitaires comme indicateur pronostique de succès thérapeutique dans le immunothérapie spécifique aux allergènes |
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| A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins;Xiufeng Li 等;《SCIENTIFIC REPORTS》;20180921;1-9 * |
| 应用同位素标记相对和绝对定量技术分析水蛭对IgA肾病血瘀证大鼠差异蛋白的表达;蓝芳 等;《湖南中医杂志》;20190731;第35卷(第7期);136-141 * |
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| CN111289425A (zh) | 2020-06-16 |
| WO2021180129A1 (fr) | 2021-09-16 |
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