CN111226685A - Planting method of fir-tree fungi - Google Patents

Abstract

The invention discloses a planting method of fir-tree fungi, which comprises the following steps: preparing strains; selecting land, and selecting a pine forest zone with a small amount of couch grass in the forest of trees growing for more than 25 years in summer, wherein the pine forest zone has certain scattered light; digging a ridge bed around the pine roots, wherein the height of the ridge bed is 5-10cm, paving nutrient materials on the ridge bed, sowing strains, covering a mulching film, and covering grass and pine needles; the formula of the nutrient material is as follows: 45% of pine sawdust, 25% of cotton seed shells, 18% of pine forest soil, 10% of wheat bran, 1% of calcium superphosphate, 0.5% of sugar and 0.5% of gypsum; after 45 days, checking whether hyphae germinate, digging a soil layer, if the hyphae form is found at a position 10-15cm below the surface of the ridge bed, indicating that the hyphae grow, removing the mulching film at the moment, and allowing the hyphae to naturally grow; picking the fruiting body 5-8 days after the fruiting body emerges. The planting method of the fir-tree fungi provided by the invention can realize the artificial cultivation of the fir-tree fungi.

Description

Planting method of fir-tree fungi

Technical Field

The invention relates to the technical field of edible fungus cultivation, in particular to a planting method of fir-tree fungi.

Background

The fir-tree fungus is a precious fungus, has high nutritional value, is rich in crude protein, crude fat, crude fiber, various amino acids, unsaturated fatty acid, nucleic acid derivatives, and also contains elements such as vitamin B1, vitamin B2, vitamin C, vitamin PP and the like, has delicious and delicious taste, also has medicinal value, has special effects of strengthening body, benefiting intestines and stomach, relieving pain, regulating qi-flowing for eliminating phlegm, expelling parasites, treating diabetes, resisting cancer and the like, and is an ideal health food for middle-aged and elderly people. Each hundred grams of the composition contains 19.3 grams of crude protein, 6.8 grams of fat, 35.5 grams of carbohydrate, 32.4 grams of fiber and also contains 18 amino acids and vitamins. The mushroom has round shape, thick meat, tender quality, fresh taste, strong fragrance and good taste. The edible fungus oil is used for frying meat slices, has excellent flavor, is fresh and incomparable when being used in soup, is prepared into fungus oil, has other taste, and is suitable for frying meat and vegetable. Fir-tree fungus is a pure natural rare and rare edible fungus and is known as 'prince in fungus'.

Because the fir fungus and the pine form mycorrhiza, the artificial cultivation difficulty is high, and few reports are reported for artificial domestication cultivation.

The invention aims to provide a planting method of fir-tree fungi.

Disclosure of Invention

The technical problem to be solved by the invention is to provide a planting method of fir-tree fungi, which can realize artificial cultivation of the fir-tree fungi.

In order to solve the problems, the technical scheme of the invention is as follows:

a planting method of fir-tree fungi comprises the following steps:

step S1, strain preparation:

step S11, preparing a mother culture medium: 200g of potatoes, 20g of glucose, 20g of agar and 1000mL of pine needle water, wherein the pine needle water is prepared by soaking pine needles in water, boiling and filtering;

step S12, fir mushroom tissue isolation: selecting fresh and tender strains with good wild shape and no discoloration, wrapping the strains with root soil by white fine cloth or white paper, separating tissues in an inoculation box, taking a small block at the intersection of a pileus and a stipe, putting mushroom meat on a mother culture medium on a test tube inclined plane by an inoculation needle, and slightly flattening;

step S13, placing the inoculated slant at 25 deg.C, culturing until the slant is full of hyphae, transferring slant strain for several times, and performing slant test tube amplification culture to obtain mother strain for preparing stock;

step S14, preparing a stock culture medium: 78% of pine sawdust, 20% of rice bran, 1% of sugar, 1% of gypsum and the balance pine needle water;

step S15, cultivating cultivars: separating the original seed tissue, placing the original seed mushroom meat on an original seed culture medium of a test tube inclined plane, and culturing at 25 ℃ until hyphae fully climb over the inclined plane to obtain a cultivated seed;

step S2, selecting: in summer, the pine forest land is selected from pine forest lands growing for more than 25 years and having a small amount of couch grass, and the pine forest land has certain scattered light;

step S3, sowing: digging a ridge bed around the pine roots, wherein the height of the ridge bed is 5-10cm, paving nutrient materials on the ridge bed, sowing strains, covering a mulching film, and covering grass and pine needles; the formula of the nutrient material is as follows: 45% of pine sawdust, 25% of cotton seed shells, 18% of pine forest soil, 10% of wheat bran, 1% of calcium superphosphate, 0.5% of sugar and 0.5% of gypsum;

step S4, post management: after 45 days, checking whether hyphae germinate, digging a soil layer, if the hyphae form is found at a position 10-15cm below the surface of the ridge bed, indicating that the hyphae grow, removing the mulching film at the moment, and allowing the hyphae to naturally grow;

and step S5, picking the fruiting bodies 5-8 days after the fruiting bodies are unearthed.

Further, the temperature of the pine forest region is 24-32 ℃.

The planting method of the fir-tree fungi provided by the invention has the beneficial effects that:

according to the planting method of the fir-tree fungi, the planting is carried out in the pine forest region, which is equivalent to natural growth, the quality of the fir-tree fungi is similar to that of wild fungi, and the nutritional value is high; meanwhile, the fungus is grown in a manual intervention mode, so that the yield of the fir-tree fungus can be improved.

Detailed Description

The following description of the present invention is provided to enable those skilled in the art to better understand the technical solutions in the embodiments of the present invention and to make the above objects, features and advantages of the present invention more comprehensible.

The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual values, and between the individual values may be combined with each other to yield one or more new ranges of values, which ranges of values should be considered as specifically disclosed herein.

A planting method of fir-tree fungi comprises the following steps:

step S1, strain preparation:

step S11, preparing a mother culture medium: 200g of potatoes, 20g of glucose, 20g of agar and 1000mL of pine needle water, wherein the pine needle water is prepared by soaking pine needles in water, boiling and filtering;

step S12, fir mushroom tissue isolation: selecting fresh and tender strains with good wild shape and no discoloration, wrapping the strains with root soil by white fine cloth or white paper, separating tissues in an inoculation box, taking a small block at the intersection of a pileus and a stipe, putting mushroom meat on a mother culture medium on a test tube inclined plane by an inoculation needle, and slightly flattening;

step S13, placing the inoculated slant at 25 deg.C, culturing until the slant is full of hyphae, transferring slant strain for several times, and performing slant test tube amplification culture to obtain mother strain for preparing stock;

step S14, preparing a stock culture medium: 78% of pine sawdust, 20% of rice bran, 1% of sugar, 1% of gypsum and the balance pine needle water;

step S15, cultivating cultivars: separating the original seed tissue, placing the original seed mushroom meat on an original seed culture medium of a test tube inclined plane, and culturing at 25 ℃ until hyphae fully climb over the inclined plane to obtain a cultivated seed;

step S2, selecting: in summer, the pine forest land is selected from pine forest lands growing for more than 25 years and having a small amount of couch grass, and the pine forest land has certain scattered light; especially in pine forest region, the temperature is preferably 24-32 ℃;

step S3, sowing: digging a ridge bed around the pine roots, wherein the height of the ridge bed is 5-10cm, paving nutrient materials on the ridge bed, sowing strains, covering a mulching film, and covering grass and pine needles; the formula of the nutrient material is as follows: 45% of pine sawdust, 25% of cotton seed shells, 18% of pine forest soil, 10% of wheat bran, 1% of calcium superphosphate, 0.5% of sugar and 0.5% of gypsum;

step S4, post management: checking whether hyphae germinate after 45 days, digging a soil layer, if the hyphae form is found at a position 10-15cm below the surface of the ridge bed, indicating that the hyphae grow, removing the mulching film at the moment, and allowing the hyphae to naturally grow, if the hyphae grow in a thunderstorm weather, the climate is proper, and the hyphae grow quickly;

step S5, picking the fruiting body 5-8 days after the fruiting body emerges; the picked fresh-keeping can be carried out for 3-4 days by using ice cubes.

According to the planting method of the fir-tree fungi, the planting is carried out in the pine forest region, which is equivalent to natural growth, the quality of the fir-tree fungi is similar to that of wild fungi, and the nutritional value is high; meanwhile, the fungus is grown in a manual intervention mode, so that the yield of the fir-tree fungus can be improved.

The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. Various changes, modifications, substitutions and alterations to these embodiments will occur to those skilled in the art without departing from the spirit and scope of the present invention.

Claims (2)

1. A planting method of fir-tree fungi is characterized by comprising the following steps:

step S1, strain preparation:

step S11, preparing a mother culture medium: 200g of potatoes, 20g of glucose, 20g of agar and 1000mL of pine needle water, wherein the pine needle water is prepared by soaking pine needles in water, boiling and filtering;

step S12, fir mushroom tissue isolation: selecting fresh and tender strains with good wild shape and no discoloration, wrapping the strains with root soil by white fine cloth or white paper, separating tissues in an inoculation box, taking a small block at the intersection of a pileus and a stipe, putting mushroom meat on a mother culture medium on a test tube inclined plane by an inoculation needle, and slightly flattening;

step S13, placing the inoculated slant at 25 deg.C, culturing until the slant is full of hyphae, transferring slant strain for several times, and performing slant test tube amplification culture to obtain mother strain for preparing stock;

step S14, preparing a stock culture medium: 78% of pine sawdust, 20% of rice bran, 1% of sugar, 1% of gypsum and the balance pine needle water;

step S15, cultivating cultivars: separating the original seed tissue, placing the original seed mushroom meat on an original seed culture medium of a test tube inclined plane, and culturing at 25 ℃ until hyphae fully climb over the inclined plane to obtain a cultivated seed;

step S2, selecting: in summer, the pine forest land is selected from pine forest lands growing for more than 25 years and having a small amount of couch grass, and the pine forest land has certain scattered light;

step S3, sowing: digging a ridge bed around the pine roots, wherein the height of the ridge bed is 5-10cm, paving nutrient materials on the ridge bed, sowing strains, covering a mulching film, and covering grass and pine needles; the formula of the nutrient material is as follows: 45% of pine sawdust, 25% of cotton seed shells, 18% of pine forest soil, 10% of wheat bran, 1% of calcium superphosphate, 0.5% of sugar and 0.5% of gypsum;

step S4, post management: after 45 days, checking whether hyphae germinate, digging a soil layer, if the hyphae form is found at a position 10-15cm below the surface of the ridge bed, indicating that the hyphae grow, removing the mulching film at the moment, and allowing the hyphae to naturally grow;

and step S5, picking the fruiting bodies 5-8 days after the fruiting bodies are unearthed.

2. The method for planting abietylum as claimed in claim 1, wherein the temperature of the pine forest is 24-32 ℃.