CN103332986A - Compatibility of culture material of agaricus brasiliensis cultivation seeds and preparation method of culture material - Google Patents
Compatibility of culture material of agaricus brasiliensis cultivation seeds and preparation method of culture material Download PDFInfo
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- CN103332986A CN103332986A CN2013102875395A CN201310287539A CN103332986A CN 103332986 A CN103332986 A CN 103332986A CN 2013102875395 A CN2013102875395 A CN 2013102875395A CN 201310287539 A CN201310287539 A CN 201310287539A CN 103332986 A CN103332986 A CN 103332986A
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- silkworm excrement
- blazei murrill
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Abstract
The invention discloses a culture material of agaricus brasiliensis cultivation seeds. The agaricus brasiliensis culture material is characterized by taking excrement of 5th instar bombyx mori as a raw material, and adding mushroom residues of agaricus brasiliensis as a bridging material used among particles so as to prepare the agaricus brasiliensis cultivation seeds. The water content of the excrement of 5th instar bombyx mori is 45%, and the water content of the bridging material is 65%. The invention also discloses a preparation method of the culture material.
Description
Technical field
Content of the present invention comprises Agaricus blazei Murrill cultivar culture material compatibility, and the making method of this culture material, belongs to edible fungus species and makes the field.
Background technology
Agaricus blazei Murrill has another name called Agaricus blazei, is a kind of rare edible mushrooms that eats the medicine dual-purpose.Agaricus blazei Murrill contains rich in protein, amino acid and polysaccharide, and the content of protein and polysaccharide is 2 times of mushroom, effect such as effective component has reducing blood-fat, antithrombotic, calm the nerves.Agaricus blazei Murrill is extremely welcome in Japan, Korea S and south east asia.
Along with the continuous expansion of Agaricus blazei Murrill cultivated area, the wheat consumption that the production of hybrid seeds is used also increases thereupon, for reducing grain consumption, must seek the reasonable substitute of the production of hybrid seeds.
There is long sericulture history in China, also is maximum in the world sericulture state, and silk cocoon production occupies world's monopoly position.Silkworm excrement is the ight soil of silkworm, is the by product of sericulture, and the annual above dry of 400kt of can collecting approximately is a rich in natural resources.
The silkworm excrement comprehensive nutrition, dried silkworm excrement contains crude protein 13.4%-14.5%, crude fat 2.2%-2.3%, robust fibre 15.8%-16.2%, nitrogen-free extract 56.9%-57.4%, trace elements such as cupric, iron and zinc also, and contain plant growth hormoness such as a small amount of alkaloid, class adrenocortical hormone, Va, Vb, Vc, Vd, Ve, nicotinic acid, indolylacetic acid and triacontanol price quote.
Agaricus blazei Murrill can utilize organic carbon source and the organic nitrogen compound in the silkworm excrement, mainly is Mierocrystalline cellulose, protein etc., and this is the most basic nutritive substance of Agaricus blazei Murrill growth.Nitrogen in the silkworm excrement, phosphorus, potassium, trace element and growth stimulant are to the mycelial certain promoter action of growing of Agaricus blazei Murrill.
Five ages, dried silkworm excrement was as the mung bean size, and particle diameter is between 2mm-4mm, and diameter is between 5mm-6mm after the water-swelling.If with silkworm excrement making in five ages Agaricus blazei Murrill cultivar, not only the bacterial classification cost descends, and has the particle shape of kernel culture, the advantage that easily scatter sowing time, makes silkworm excrement obtain innoxious use simultaneously.
Summary of the invention
The present invention has following two aspect contents:
1, a kind of Agaricus blazei Murrill cultivar culture material compatibility
2, the making method of culture material
The technical solution adopted in the present invention is:
A kind of Agaricus blazei Murrill cultivar culture material compatibility has following component and quality proportioning: silkworm excrement 88%-92%, and Agaricus blazei chaff 4%-8%, terra alba 3%, sucrose 1% all is the quality of dry-matter, the proportioning sum is 100%.
Silkworm excrement: fresh nothing go mouldy five age silkworm excrement dry and sieve, it is standby to remove the residual leaf of mulberry and other foreign material.Nitrogenous (N) 2.97% of dried silkworm excrement, phosphorus (P
2O
5) 1.03%, potassium (K
2O) 3.52%.
Agaricus blazei chaff: be the leftover bits and pieces bacterium material after the Agaricus blazei Murrill cultivation, with its particle that is ground into particle diameter 1mm-2mm, dry standby.
Terra alba: be common commercially available product.Can play and stablize the culture material potential of hydrogen, increase the effect of calcium, sulphur nutritive ingredient.
Sucrose: be that Agaricus blazei Murrill is cultivated replenishing of initial stage carbon source, can induce the generation of extracellular enzyme simultaneously, accelerate coarse-fibred utilization.
The making method of culture material comprises the steps:
1. soak: dried silkworm excrement soaks 15min-20min with clear water, and soft slightly and cannot not pinch mashedly degree of being with silkworm excrement, water content was 45% (being puffy);
2. spice: bacterium chaff, terra alba siccative are mixed thoroughly, and the sucrose water spice with 1% makes the water content of bacterium chaff reach 65%, then stirs with the silkworm excrement that had soaked;
3. bottling: with the silkworm excrement culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
4. sterilize, cool off: culturing bottle is carried out autoclaving, make 100 ℃ of sterilization 1h earlier, make 121 ℃ of autoclaving 1.5h then; The type of cooling is: naturally cool to below 25 ℃.
Culture material is after inserting original seed, and culturing room's temperature control is at 20 ℃-22 ℃, and relative air humidity is controlled at 60%-75%, and through the cultivation of 24d-25d, mycelia is covered with bottle, and 3d-5d is cultivated in after-ripening then, just can be used for producing.
The present invention is raw material with silkworm silkworm excrement in five ages, admixes the Agaricus blazei chaff as intergranular bridging material, makes the Agaricus blazei Murrill cultivar.Compare with the wheat kind, its advantage is: the one, and the silkworm excrement comprehensive nutrition, mycelial growth is vigorous, and spawn activity is strong, is difficult for aging; The 2nd, the bacterial classification of preparation has the particle shape of kernel culture, and easily scatter sowing time; The 3rd, the sterilization link of the production of hybrid seeds has been killed pathogenic bacteria in the silkworm excrement, has purified the hygienic condition in silkworm district; The 4th, replace wheat with silkworm excrement, saved grain and feed, widened the raw material channel, reduced the bacterial classification cost.
Embodiment
Mode 1: Agaricus blazei Murrill cultivar culture material comprises following component and quality proportioning in the present embodiment: silkworm excrement 88%, and Agaricus blazei chaff (the bacterium material is pulverized) 8%, terra alba 3%, sucrose 1% all is the quality of dry-matter, the proportioning sum is 100%.
Making method:
1. soak: dried silkworm excrement soaks 17min with clear water,, water content was 45% (being puffy);
2. spice: bacterium chaff, terra alba siccative are mixed thoroughly, and the sucrose water spice with 1% makes the water content of bacterium chaff reach 65%, then stirs with the silkworm excrement that had soaked;
3. bottling: with the silkworm excrement culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
4. sterilize, cool off: culturing bottle is carried out autoclaving, make 100 ℃ of sterilization 1h earlier, make 121 ℃ of autoclaving 1.5h then; The type of cooling is: naturally cool to below 25 ℃.
Culture material is after inserting original seed, and culturing room's temperature control is at 22 ℃, and relative air humidity is controlled 65%, and through the cultivation of 24d, mycelia is covered with bottle, and 4d is cultivated in after-ripening then, just can be used for producing.
Mode 2: Agaricus blazei Murrill cultivar culture material comprises following component and quality proportioning in the present embodiment: silkworm excrement 92%, and Agaricus blazei chaff (the bacterium material is pulverized) 4%, terra alba 3%, sucrose 1% all is the quality of dry-matter, the proportioning sum is 100%.Making method is with reference to mode 1.
Mode 3 (control group): Agaricus blazei Murrill wheat kind culture material: wheat 97%, terra alba 3%.
By following table as can be known, the sprout time of three kinds of mode Agaricus blazei Murrill mycelia is 18h, and mycelial growth is dense, and the full bottle of silkworm excrement culture material time ratio control group shortens 3d-4d; Observe the aging index (mycelia color, yellow water amount) of bacterial classification, the silkworm excrement culture material of all modes is when sending out bacterium 60d, and mycelia is still pure white, no yellow water, and the mycelia of kernel culture is dark yellow, and more yellow water is arranged.
Table three group culture material is to the influence of Agaricus blazei Murrill cultivar mycelial growth
Annotate: mycelial density with ++, +++and ++ ++ expression ,+number more multilist shows that mycelial density is more high.
Claims (5)
1. Agaricus blazei Murrill cultivar culture material compatibility is characterized in that containing following component and quality proportioning: silkworm excrement 88%-92%, and Agaricus blazei chaff 4%-8%, terra alba 3%, sucrose 1% all is the quality of dry-matter, the proportioning sum is 100%.
2. Agaricus blazei Murrill cultivar culture material according to claim 1, it is characterized in that described silkworm excrement be fresh nothing go mouldy five age silkworm excrement, dry and sieve, it is standby to remove the residual leaf of mulberry and other foreign material.
3. Agaricus blazei Murrill cultivar culture material according to claim 1 is characterized in that described Agaricus blazei chaff, is the leftover bits and pieces bacterium material after the Agaricus blazei Murrill cultivation, and it is ground into the particle of 1mm-2mm.
4. the making method of the Agaricus blazei Murrill cultivar culture material described in the claim 1-3 is characterized in that:
1. soak: dried silkworm excrement soaks 15min-20min with clear water, and soft slightly and cannot not pinch mashedly degree of being with silkworm excrement, water content was 45% (being puffy);
2. spice: bacterium chaff, terra alba siccative are mixed thoroughly, and the sucrose water spice with 1% makes the water content of bacterium chaff reach 65%, then stirs with the silkworm excrement that had soaked;
3. bottling: with the silkworm excrement culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
4. sterilize, cool off: culturing bottle is carried out autoclaving, make 100 ℃ of sterilization 1h earlier, make 121 ℃ of autoclaving 1.5h then; The type of cooling is: naturally cool to below 25 ℃.
5. make cultivar with the agaricus blazei culture material described in the claim 1-4, after it is characterized in that inserting original seed, culturing room's temperature control is at 20 ℃-22 ℃, relative air humidity control is at 60%-75%, cultivation through 24d-25d, mycelia is covered with bottle, and 3d-5d is cultivated in after-ripening then, just can be used for producing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106242682A (en) * | 2016-08-04 | 2016-12-21 | 贵州宏华农业发展有限公司 | Agaricus blazei Murrill Primary spawn material and manufacture method |
| CN111226685A (en) * | 2019-12-07 | 2020-06-05 | 湖南忠食菌业有限公司 | Planting method of fir-tree fungi |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613004A (en) * | 2012-04-14 | 2012-08-01 | 河北荣珍食用菌股份有限公司 | Method using pleurotus nebrodensis waste to cultivate flammulina velutipes |
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2013
- 2013-06-29 CN CN201310287539.5A patent/CN103332986B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613004A (en) * | 2012-04-14 | 2012-08-01 | 河北荣珍食用菌股份有限公司 | Method using pleurotus nebrodensis waste to cultivate flammulina velutipes |
Non-Patent Citations (1)
| Title |
|---|
| 俞小华等: "利用桑枝蚕粪栽培食用菌(续一)", 《蚕桑通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106242682A (en) * | 2016-08-04 | 2016-12-21 | 贵州宏华农业发展有限公司 | Agaricus blazei Murrill Primary spawn material and manufacture method |
| CN111226685A (en) * | 2019-12-07 | 2020-06-05 | 湖南忠食菌业有限公司 | Planting method of fir-tree fungi |
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| CN103332986B (en) | 2015-03-25 |
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