Disclosure of Invention
Therefore, the invention provides the automatic living cell incubator, which can prevent the static cell inside from adhering to the wall after swinging the culture bottle, does not need stirring equipment, prevents the cell from dying and improves the survival rate of cell culture.
The technical scheme of the invention is realized as follows:
an automatic incubator for living cells comprises an incubator body, wherein the incubator body comprises a culture chamber, a U-shaped support, a culture bottle, a swing mechanism and a reciprocating mechanism are arranged in the culture chamber, the U-shaped support is arranged in the culture chamber in a sliding mode and comprises a bottom plate and two side plates, the culture bottle is movably connected between the two side plates, the swing mechanism is arranged in one of the side plates and connected with the culture bottle, and the reciprocating mechanism drives the U-shaped support to move in a reciprocating mode; the upper ends of the two sides of the culture bottle are provided with a first connecting end and a second connecting end, the first connecting end is movably connected with one side plate, and the second connecting end extends into the other side plate; the swing mechanism comprises a driven gear arranged on the second connecting end, first half gears symmetrically arranged on two sides of the driven gear and rotating motors connected with the first half gears, and the rotating motors sequentially drive the first half gears connected with the rotating motors to be meshed with the driven gear.
Preferably, reciprocating mechanism includes driving motor, half side gear of second and rack, the rack sets up surface about the bottom plate is inside, driving motor sets up in cultivateing the chamber, and its output shaft stretches into the bottom plate inside, half side gear of second sets up on driving motor's output shaft, driving motor drives half side gear of second and is connected with two rack toothing in proper order.
Preferably, the side wall of the bottom plate is provided with a strip-shaped groove, and an output shaft of the driving motor penetrates through the strip-shaped groove to enter the inside of the bottom plate.
Preferably, the lower surface of the bottom plate is provided with a slide bar, the bottom surface of the culture cavity is provided with a sliding groove, and the slide bar is positioned in the sliding groove.
Preferably, the side plate is provided with a groove, the other side plate is provided with a through groove communicated with the inside of the side plate, the first connecting end is arranged in the groove, and the second connecting end penetrates through the through groove.
Preferably, a stop block is arranged in the through groove, a limit block is arranged on the second connecting end, and the stop block is positioned on the rotation stroke of the limit block.
Preferably, the culture bottle comprises a liquid inlet and a liquid outlet, and the liquid inlet and the liquid outlet are both provided with an upper cover.
Preferably, including control panel and temperature sensor, control panel sets up in the box side, temperature sensor sets up in cultivateing the chamber, temperature sensor is connected with control panel electricity.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides an automatic living cell incubator which is suitable for suspension culture of cells, wherein a culture bottle is arranged on a U-shaped support, a swing mechanism arranged in a side plate of the U-shaped support can swing the culture bottle, so that culture solution in the culture bottle can swing in a wave shape, the culture solution is not in a static state for a long time, the suspension state of the cells is kept, the effect of the cell culture is ensured, meanwhile, a reciprocating mechanism is also arranged, the U-shaped support can reciprocate, the culture solution can be further stirred, an external stirring device is not required to extend into the culture solution in the stirring process, the cells are prevented from being dead due to stirring of the stirring device, and the survival rate of the cells is improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only preferred embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a schematic view of an automatic incubator for living cells according to the present invention;
FIG. 2 is an enlarged view taken at A in FIG. 1;
FIG. 3 is a schematic diagram of a rocking mechanism of an automatic living cell incubator according to the present invention;
FIG. 4 is a schematic diagram showing the structure of a reciprocating mechanism of an automatic living cell incubator according to the present invention;
in the figure, 1 is a box body, 2 is a culture cavity, 3 is a U-shaped bracket, 4 is a culture bottle, 5 is a bottom plate, 6 is a side plate, 7 is a first connecting end, 8 is a second connecting end, 9 is a driven gear, 10 is a first half-side gear, 11 is a rotating motor, 12 is a driving motor, 13 is a second half-side gear, 14 is a rack, 15 is a strip-shaped groove, 16 is a sliding strip, 17 is a sliding groove, 18 is a groove, 19 is a through groove, 20 is a stop block, 21 is a limiting block, 22 is a liquid outlet, 23 is a liquid inlet, 24 is an upper cover, 25 is a control panel, and 26 is a temperature sensor.
Detailed Description
For a better understanding of the technical content of the present invention, a specific embodiment is provided below, and the present invention is further described with reference to the accompanying drawings.
Referring to fig. 1 to 4, the automatic incubator for living cells provided by the invention comprises an incubator body 1, wherein the incubator body 1 comprises a culture chamber 2, a U-shaped support 3, a culture bottle 4, a swing mechanism and a reciprocating mechanism are arranged in the culture chamber 2, the U-shaped support 3 is slidably arranged in the culture chamber 2 and comprises a bottom plate 5 and two side plates 6, the culture bottle 4 is movably connected between the two side plates 6, the swing mechanism is arranged in one of the side plates 6 and connected with the culture bottle 4, and the reciprocating mechanism drives the U-shaped support 3 to reciprocate; the upper ends of two sides of the culture bottle 4 are provided with a first connecting end 7 and a second connecting end 8, the first connecting end 7 is movably connected with one side plate 6, and the second connecting end 8 extends into the other side plate 6; the swing mechanism comprises a driven gear 9 arranged on the second connecting end 8, first half-side gears 10 symmetrically arranged on two sides of the driven gear 9 and rotating motors 11 connected with the first half-side gears 10, wherein the rotating motors 11 sequentially drive the first half-side gears 10 connected with the rotating motors to be meshed with the driven gear 9.
The invention relates to an automatic incubator for living cells, which is used for suspension culture of the cells, a culture bottle 4 is arranged on a U-shaped bracket 3 in a culture cavity 2, the U-shaped bracket 3 not only serves as a support body of the culture bottle 4, but also is internally provided with a swing mechanism, the U-shaped bracket 3 is a U-shaped structure consisting of two side plates 6 and a bottom plate 5, a first connecting end 7 and a second connecting end 8 at two sides of the culture bottle 4 are respectively movably connected with the two side plates 6, the first connecting end 7 and the second connecting end 8 are positioned above the side surface of the culture bottle 4, when the culture bottle 4 rotates in a reciprocating way by taking the connecting line of the first connecting end 7 and the second connecting end 8 as an axis, the phenomenon of front-back swing can be formed, the invention arranges the swing mechanism in one side plate 6 of the U-shaped bracket 3, the swing mechanism is connected with the second connecting end 8, and drives the second connecting end 8 to rotate in front-back directions in turn, thereby realize rocking of blake bottle 4 for the culture solution in the blake bottle 4 forms wavy stirring, thereby breaks the quiescent condition of culture solution, prevents that the cell from taking place adherence phenomenon, keeps the suspended state of cell, thereby provides strong growing environment for the cell, simultaneously owing to need not set up agitated vessel in blake bottle 4, can prevent that agitated vessel's stirring from causing the death of cell, also can prevent that the heat that agitated vessel produced from influencing the environment of culture solution.
The swing mechanism comprises two rotating motors 11, two first half gears 10 and one driven gear 9, wherein the driven gear 9 is arranged on the part, extending into the side plate 6, of the second connecting end 8, the two first half gears 10 are symmetrically arranged on two sides of the driven gear 9, the two rotating motors 11 are respectively connected with the two first half gears 10, the driven gear 9 can be meshed with one first half gear 10 at the same time, and the two rotating motors 11 can sequentially drive the first half gears 10 to be meshed with the driven gear 9, so that the second connecting end 8 and the culture bottle 4 can rotate in two different directions to form swing.
Meanwhile, the invention also adds the function of pushing the culture bottle 4 to reciprocate left and right, and can shake and stir the culture solution in the culture bottle 4 by matching with the swinging of the upper culture bottle 4, wherein the reciprocating mechanism can push the whole U-shaped bracket 3 to slide in the culture cavity 2, and after matching with the swinging mechanism, the culture solution can shake and stir all around, so as to ensure that the cells can be in a suspension state.
Preferably, the reciprocating mechanism comprises a driving motor 12, a second half gear 13 and a rack 14, the rack 14 is arranged on the upper surface and the lower surface inside the bottom plate 5, the driving motor 12 is arranged in the culture cavity 2, an output shaft of the driving motor extends into the bottom plate 5, the second half gear 13 is arranged on the output shaft of the driving motor 12, and the driving motor 12 drives the second half gear 13 to be sequentially meshed with the two racks 14.
The reciprocating mechanism is realized by a driving motor 12, when the driving motor 12 drives the second half gear 13 to rotate, the second half gear 13 can be sequentially meshed and connected with two racks 14 arranged on the upper surface and the lower surface inside the bottom plate 5, and the driving motor 12 is fixed in the culture cavity 2, so that the second half gear 13 cannot move in the horizontal direction, and when the driving motor 12 rotates, the bottom plate 5 can be driven to move in the left direction and the right direction, so that the reciprocating motion of the U-shaped support 3 is realized, and the culture bottle 4 is driven to reciprocate left and right.
Preferably, the side wall of the bottom plate 5 is provided with a strip-shaped groove 15, and an output shaft of the driving motor 12 penetrates through the strip-shaped groove 15 and enters the bottom plate 5.
Because the bottom plate 5 receives the drive of driving motor 12 to carry out reciprocating motion, consequently, in order to prevent that the output shaft of driving motor 12 from hindering its motion when bottom plate 5 moves, set up bar groove 15 in the side of bottom plate 5, inside driving motor 12's output shaft can pass bar groove 15 and enter into bottom plate 5, when bottom plate 5 carries out the horizontal slip, bar groove 15 can slide along driving motor 12 output shaft.
Preferably, the lower surface of the bottom plate 5 is provided with a slide bar 16, the bottom surface of the culture cavity 2 is provided with a sliding groove 17, and the slide bar 16 is positioned in the sliding groove 17.
In order to ensure that the bottom plate 5 does not deviate when sliding, a sliding strip 16 is arranged on the lower surface of the bottom plate 5, the sliding strip 16 is embedded into a sliding groove 17 on the bottom surface of the culture cavity 2, and when the bottom plate 5 slides, the sliding strip 16 can slide along the sliding groove 17.
Preferably, a groove 18 is formed in the side plate 6, a through groove 19 communicated with the inside of the side plate 6 is formed in the other side plate 6, the first connecting end 7 is arranged in the groove 18, and the second connecting end 8 penetrates through the through groove 19.
Because the culture bottle 4 needs to swing, corresponding structures are arranged on the two side plates 6 and are respectively a groove 18 and a through groove 19, the groove 18 is of a closed structure, the first connecting end 7 can be inserted into the groove 18 and can rotate in the groove 18, the second connecting end 8 penetrates through the through groove 19 to enter the side plate 6, and the part of the second connecting end 8 penetrating into the side plate 6 is provided with a driven gear 9 and is connected with other parts of the swing mechanism through the driven gear 9.
Preferably, a stopper 20 is disposed in the through groove 19, a limiting block 21 is disposed on the second connecting end 8, and the stopper 20 is located on a rotation stroke of the limiting block 21.
In order to prevent that the amplitude that the blake bottle 4 leads to rocking because inertia in the in-process of rocking is too big, dog 20 has been set up in logical groove 19, dog 20 has two, the symmetry sets up the both sides at second link 8, limit block 21 has been set up on second link 8 simultaneously, after first half gear 10 rotates and drives second link 8 and rotate and accomplish, when first half gear 10 left driven gear 9, in order to prevent that blake bottle 4 from continuing the swing under inertial effect, the dog 20 of setting can prevent that limit block 21 from continuing to rotate.
Preferably, the culture bottle 4 comprises a liquid inlet 23 and a liquid outlet 22, and both the liquid inlet 23 and the liquid outlet 22 are provided with an upper cover 24.
The liquid inlet 23 and the liquid outlet 22 are used for adding culture solution to the culture bottle 4 or removing waste liquid, and the upper cover 24 can prevent the culture bottle 4 from being thrown out of the liquid inlet 23 or the liquid outlet 22 in the swinging process.
Preferably, the device comprises a control panel 25 and a temperature sensor 26, wherein the control panel 25 is arranged on the side surface of the box body 1, the temperature sensor 26 is arranged in the culture cavity 2, and the temperature sensor 26 is electrically connected with the control panel 25.
The control panel 25 can be used for controlling the start of the driving motor 12 and the rotating motor 11, and the temperature sensor 26 arranged in the culture chamber 2 can detect the temperature in the culture chamber 2 and display the temperature information on the control panel 25 for monitoring the temperature environment of the cell culture.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.