CN114395478A - Be used for biological cell culture bottle rotating machine - Google Patents
Be used for biological cell culture bottle rotating machine Download PDFInfo
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- CN114395478A CN114395478A CN202210063937.8A CN202210063937A CN114395478A CN 114395478 A CN114395478 A CN 114395478A CN 202210063937 A CN202210063937 A CN 202210063937A CN 114395478 A CN114395478 A CN 114395478A
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- incubator
- liquid
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- bottle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
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- Biotechnology (AREA)
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- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a bottle rotating machine for biological cell culture, which relates to the technical field of biological cell culture and comprises a bracket and an incubator arranged at the top of the bracket, wherein a driving mechanism for driving the movement of a culture bottle is rotationally arranged in the incubator, a liquid adding assembly for providing nutrient solution for the culture bottle is arranged below the inner wall of the incubator, and the liquid adding assembly is communicated with a liquid storage tank on the bracket. In the invention, the driving mechanism arranged in the incubator drives the culture bottle to rotate circumferentially and simultaneously drives the culture bottle to move up and down, so that the biological cells and the nutrient solution in the culture bottle can be mixed circumferentially and can also form turbulent mixing, and the efficiency of the biological cells for absorbing the nutrient solution is improved; meanwhile, the driving mechanism drives the liquid feeding assembly to automatically feed a small amount of nutrient solution to the culture bottle for many times, so that the workload of operators is reduced.
Description
Technical Field
The invention relates to the technical field of biological cell culture, in particular to a bottle rotating machine for biological cell culture.
Background
Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells.
Current cell culture cultivates through the blake bottle mostly, and needs rotate the blake bottle among the current culture process, the cell that makes the blake bottle in culture can be more abundant mix with the nutrient solution, absorb the nutrition, but current blake bottle is mostly only circumferential direction for cell and nutrient solution mixing efficiency in the blake bottle is lower, cell culture needs artifical a small amount of many times to add the nutrient solution in the blake bottle simultaneously, leads to operating personnel's working strength great.
Disclosure of Invention
The invention aims to solve the problems of the prior art, and provides a bottle rotating machine for biological cell culture.
In order to achieve the purpose, the invention adopts the following technical scheme:
a rotating bottle machine for biological cell culture comprises a support and an incubator arranged at the top of the support, wherein a driving mechanism for driving the movement of a culture bottle is rotationally arranged in the incubator, a liquid adding assembly for providing nutrient solution for the culture bottle is arranged below the inner wall of the incubator, and the liquid adding assembly is communicated with a liquid storage tank on the support;
the driving mechanism comprises a plurality of rotating discs which are rotatably arranged on the inner wall of the rear side of the incubator, the upper end and the lower end of each rotating disc are respectively provided with a fixing plate extending forwards, a fixed clamping seat is arranged above the fixing plate at the lower part, a movable clamping seat is arranged below the fixing plate at the upper part, the plurality of rotating discs are rotatably connected with the incubator through rotating shafts, the plurality of rotating shafts synchronously rotate through the matching of belt wheels and synchronous belts, and any one rotating shaft is driven by a motor;
the surface of the rotating shaft is provided with a cam, the cam is positioned behind the turntable, a sliding plate which is contacted with the cam is arranged in the incubator in a vertically sliding manner, the sliding plate is positioned below the cam, the upper part of one end, far away from the cam, of the sliding plate is detachably connected with a connecting rod, the top of the connecting rod is provided with a connecting sleeve, and the connecting sleeve is rotatably connected with the outer wall of the culture bottle;
the liquid feeding assembly comprises a plurality of liquid feeding assemblies, one pair of liquid feeding assemblies are arranged below the culture bottle, each liquid feeding assembly comprises a sealing box arranged below the inner wall of the culture box and a piston arranged in the sealing box in a sliding manner, a push rod at the top of the piston is detachably connected with the bottom of a sliding plate, a liquid outlet hole is formed in the right side of the sealing box, a liquid outlet one-way valve is embedded in the liquid outlet hole, the liquid outlet hole is communicated with a liquid inlet at the top of a connecting sleeve through a hose, a liquid passing port communicated with the liquid inlet is formed in the surface of the culture bottle, a liquid inlet hole is formed in the rear side of the sealing box, a liquid inlet one-way valve is embedded in the liquid inlet hole, and the liquid inlet hole is communicated with the liquid storage box through a water pipe;
the eccentric part of the cam is arranged in the same direction with the movable clamping seat.
Further, fixed holder and movable holder are two, and the symmetry sets up both ends around the fixed plate, one side that fixed holder and movable holder are close to the blake bottle all sets up to the pitch arc.
Further, the movable clamping seat is connected with the upper fixing plate through a return spring.
Furthermore, a sealing rubber ring matched with the liquid passing port is arranged on the inner wall of the connecting sleeve far away from the liquid inlet.
Further, the slide all is provided with the slider about the slide, the spout that is used for the slider to slide from top to bottom is seted up to the incubator inner wall left and right sides.
Compared with the prior art, the invention has the following beneficial effects:
in the invention, the driving mechanism arranged in the incubator drives the culture bottle to rotate circumferentially and simultaneously drives the culture bottle to move up and down, so that the biological cells and the nutrient solution in the culture bottle can be mixed circumferentially and can also form turbulent mixing, and the efficiency of the biological cells for absorbing the nutrient solution is improved; meanwhile, the driving mechanism drives the liquid feeding assembly to automatically feed a small amount of nutrient solution to the culture bottle for many times, so that the workload of operators is reduced.
Drawings
FIG. 1 is a front view of a rotary bottle-rotating machine for biological cell culture according to the present invention;
FIG. 2 is a side view of a spinner for biological cell culture according to the present invention;
FIG. 3 is a state diagram of the motion of FIG. 2;
FIG. 4 is a schematic view of the installation position of the connecting sleeve and the culture bottle in the biological cell culture bottle rotating machine according to the present invention;
fig. 5 is a movement state diagram of fig. 4.
In the figure: 1. a support; 2. an incubator; 3. a culture bottle; 31. a liquid passing port; 4. a drive mechanism; 41. a turntable; 42. a fixing plate; 43. a fixed clamp base; 44. a movable holder; 45. a cam; 46. connecting sleeves; 47. a connecting rod; 48. a slide plate; 49. sealing the rubber ring; 5. a liquid adding assembly; 51. a piston; 52. a sealing box; 53. a hose; 6. a liquid storage tank.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-5, a rotating bottle machine for biological cell culture comprises a support 1 and an incubator 2 arranged at the top of the support 1, wherein a driving mechanism 4 for driving the movement of the incubator 3 is rotatably arranged in the incubator 2, a liquid adding assembly 5 for providing nutrient solution for the incubator 3 is arranged below the inner wall of the incubator 2, and the liquid adding assembly 5 is communicated with a liquid storage tank 6 on the support 1;
the driving mechanism 4 comprises a plurality of rotating discs 41 which are rotatably arranged on the inner wall of the rear side of the incubator 2, the upper end and the lower end of each rotating disc 41 are respectively provided with a fixing plate 42 which extends forwards, a fixed clamping seat 43 is arranged above the lower fixing plate 42, a movable clamping seat 44 is arranged below the upper fixing plate 42, the plurality of rotating discs 41 are rotatably connected with the incubator 2 through rotating shafts, the plurality of rotating shafts synchronously rotate through the matching of belt wheels and synchronous belts, and any rotating shaft is driven by a motor;
the surface of the rotating shaft is provided with a cam 45, the cam 45 is positioned behind the rotating disc 41, a sliding plate 48 which is contacted with the cam 45 is arranged in the incubator 2 in a vertically sliding manner, the sliding plate 48 is positioned below the cam 45, the upper part of one end, far away from the cam 45, of the sliding plate 48 is detachably connected with a connecting rod 47, the top of the connecting rod 47 is provided with a connecting sleeve 46, and the connecting sleeve 46 is rotatably connected with the outer wall of the culture bottle 3;
the liquid adding assemblies 5 are multiple, one pair of liquid adding assemblies are arranged below the culture bottle 3, each liquid adding assembly 5 comprises a sealing box 52 arranged below the inner wall of the culture box 2 and a piston 51 arranged in the sealing box 52 in a sliding mode, a push rod at the top of the piston 51 is detachably connected with the bottom of a sliding plate 48, a liquid outlet hole is formed in the right side of the sealing box 52, a liquid outlet one-way valve is embedded in the liquid outlet hole, the liquid outlet hole is communicated with a liquid inlet at the top of a connecting sleeve 46 through a hose 53, a liquid passing port 31 communicated with the liquid inlet is formed in the surface of the culture bottle 3, a liquid inlet hole is formed in the rear side of the sealing box 52, a liquid inlet one-way valve is embedded in the liquid inlet hole, and the liquid inlet hole is communicated with the liquid storage box 6 through a water pipe;
the eccentric part of the cam 45 is arranged in the same direction as the movable clamp seat 44.
Further, the fixed type clamping seats 43 and the movable type clamping seats 44 are two and symmetrically arranged at the front end and the rear end of the fixing plate 42, and arc lines are arranged on one sides of the fixed type clamping seats 43 and the movable type clamping seats 44 close to the culture bottles 3.
Further, the movable holder 44 is connected to the upper fixing plate 42 by a return spring.
Further, a sealing rubber ring 49 matched with the liquid passing port 31 is arranged on the inner wall of the connecting sleeve 46 far away from the liquid inlet.
Further, the slide 48 left and right sides all is provided with the slider, and the spout that is used for the slider to slide from top to bottom is seted up to the incubator 2 inner wall left and right sides.
The working principle is as follows: when in use, firstly, the culture bottle 3 containing biological cells slides into the space between the fixed holder 43 and the movable holder 44, at the moment, the movable holder 44 is driven to be close to the fixed holder 43 under the action of the return spring, so as to hold the culture bottle 3 between the fixed holder 43 and the movable holder 44, then the connecting rod 47 at the bottom of the connecting sleeve 46 on the culture bottle 3 is connected with the top of the sliding plate 48, then the push rod at the top of the piston 51 in the sealing box 52 below the culture bottle 3 is connected with the bottom of the sliding plate 48, and meanwhile, the hose 53 at the right side of the sealing box 52 is communicated with the liquid inlet at the top of the connecting sleeve 46;
further, the starting motor drives the rotating shafts to rotate, the rotating shafts are driven to synchronously rotate under the action of the belt wheel and the synchronous belt, and then the rotating discs 41 are driven to rotate, so that the culture bottles 3 at the front ends of the rotating discs 41 rotate, biological cells in the culture bottles 3 are circumferentially mixed with nutrient solution, and the efficiency of the biological cells for absorbing the nutrient solution is improved;
when the eccentric position of the cam 45 is far away from the sliding plate 48, the culture bottle 3 is driven to move close to the fixed clamping seat 43 under the action of the reset spring, and then the connecting sleeve 46 drives the sliding plate 48 to move upwards, so that the biological cells in the culture bottle 3 and the nutrient solution move upwards and downwards to form turbulent flow type mixing, and the efficiency of the biological cells for absorbing the nutrient solution is further improved;
in the process that the sliding plate 48 moves downwards, the sliding plate 48 drives the piston 51 to move downwards to extrude the nutrient solution in the sealing box 52, so that the nutrient solution enters the liquid inlet of the connecting sleeve 46 from the hose 53, and along with the rotation of the culture bottle 3, when the liquid passing port 31 on the culture bottle 3 is communicated with the liquid inlet, the nutrient solution in the liquid inlet enters the culture bottle 3 from the liquid passing port 31; in the process that the sliding plate 48 moves upwards, the piston 51 is driven to move upwards, and at the moment, the nutrient solution in the liquid storage box 6 is pumped into the sealing box 52 through the water pipe, so that the nutrient solution is automatically added to the culture bottle 3.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. The rotating bottle machine for biological cell culture is characterized by comprising a support (1) and an incubator (2) arranged at the top of the support (1), wherein a driving mechanism (4) for driving the incubator (3) to move is rotationally arranged in the incubator (2), a liquid adding assembly (5) for providing nutrient solution for the incubator (3) is arranged below the inner wall of the incubator (2), and the liquid adding assembly (5) is communicated with a liquid storage box (6) on the support (1);
the driving mechanism (4) comprises a plurality of rotating discs (41) which are rotatably arranged on the inner wall of the rear side of the incubator (2), fixing plates (42) which extend forwards are arranged at the upper end and the lower end of each rotating disc (41), a fixed clamping seat (43) is arranged above the fixing plate (42) at the lower part, a movable clamping seat (44) is arranged below the fixing plate (42) at the upper part, the plurality of rotating discs (41) are rotatably connected with the incubator (2) through rotating shafts, the rotating shafts synchronously rotate through the matching of belt wheels and synchronous belts, and any one rotating shaft is driven by a motor;
the surface of the rotating shaft is provided with a cam (45), the cam (45) is positioned behind the rotating disc (41), a sliding plate (48) which is in contact with the cam (45) is arranged in the incubator (2) in a vertically sliding mode, the sliding plate (48) is positioned below the cam (45), the upper portion of one end, far away from the cam (45), of the sliding plate (48) is detachably connected with a connecting rod (47), the top of the connecting rod (47) is provided with a connecting sleeve (46), and the connecting sleeve (46) is rotatably connected with the outer wall of the culture bottle (3);
the liquid feeding components (5) are arranged below the culture bottle (3) one by one, each liquid feeding component (5) comprises a sealing box (52) arranged below the inner wall of the culture box (2) and a piston (51) arranged in the sealing box (52) in a sliding mode, a push rod at the top of the piston (51) is detachably connected with the bottom of a sliding plate (48), a liquid outlet hole is formed in the right side of the sealing box (52), a liquid outlet one-way valve is embedded in the liquid outlet hole, the liquid outlet hole is communicated with a liquid inlet in the top of a connecting sleeve (46) through a hose (53), a liquid passing port (31) communicated with the liquid inlet is formed in the surface of the culture bottle (3), a liquid inlet hole is formed in the rear side of the sealing box (52), a liquid inlet one-way valve is embedded in the liquid inlet hole, and the liquid inlet hole is communicated with a liquid storage box (6) through a water pipe;
the eccentric part of the cam (45) is arranged in the same direction with the movable clamping seat (44).
2. The rotary machine for biological cell culture according to claim 1, wherein the fixed holder (43) and the movable holder (44) are two and symmetrically arranged at the front and rear ends of the fixing plate (42), and one sides of the fixed holder (43) and the movable holder (44) close to the culture bottle (3) are both arranged to be arc lines.
3. The spinner for biological cell culture as claimed in claim 2, wherein the movable holders (44) are connected with the upper fixing plate (42) by return springs.
4. The rotary bottle-rotating machine for biological cell culture is characterized in that a sealing rubber ring (49) matched with the liquid passing port (31) is arranged on the inner wall of the connecting sleeve (46) far away from the liquid inlet port.
5. The biological cell culture bottle rotating machine according to claim 1, wherein sliding blocks are arranged on the left side and the right side of the sliding plate (48), and sliding grooves for the sliding blocks to slide up and down are formed on the left side and the right side of the inner wall of the incubator (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210063937.8A CN114395478B (en) | 2022-01-20 | 2022-01-20 | Bottle rotating machine for biological cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210063937.8A CN114395478B (en) | 2022-01-20 | 2022-01-20 | Bottle rotating machine for biological cell culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114395478A true CN114395478A (en) | 2022-04-26 |
| CN114395478B CN114395478B (en) | 2023-09-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210063937.8A Active CN114395478B (en) | 2022-01-20 | 2022-01-20 | Bottle rotating machine for biological cell culture |
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| Country | Link |
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| CN (1) | CN114395478B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111575149A (en) * | 2020-05-09 | 2020-08-25 | 潘齐辉 | Culture device and culture method for slow-release aerobic strains |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060035368A1 (en) * | 2004-08-13 | 2006-02-16 | Malinge David S | Shaking system for cell culture |
| US20120083029A1 (en) * | 2009-06-09 | 2012-04-05 | Takashi Tsumura | Rotating culture vessel and automatic cell culture apparatus using same |
| CN210438747U (en) * | 2019-07-18 | 2020-05-01 | 中科细胞科技(广州)有限公司 | Liquid supplementing device for cell culture |
| CN111172032A (en) * | 2020-02-19 | 2020-05-19 | 海口健康岛生物科技有限公司 | Automatic incubator for living cells |
| CN212174969U (en) * | 2020-03-17 | 2020-12-18 | 南京诺尔曼生物技术有限公司 | Constant temperature incubator is used in biological cell culture |
| CN112457981A (en) * | 2020-11-27 | 2021-03-09 | 河南灿方机械设备科技有限公司 | Cell culture device for tissue engineering and use method thereof |
| CN214142372U (en) * | 2020-12-17 | 2021-09-07 | 天津市中奥天元科技发展有限公司 | Liquid supplementing device for cell culture |
| CN113801769A (en) * | 2021-09-18 | 2021-12-17 | 新乡医学院 | Culture apparatus for medical science experiments |
-
2022
- 2022-01-20 CN CN202210063937.8A patent/CN114395478B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060035368A1 (en) * | 2004-08-13 | 2006-02-16 | Malinge David S | Shaking system for cell culture |
| US20120083029A1 (en) * | 2009-06-09 | 2012-04-05 | Takashi Tsumura | Rotating culture vessel and automatic cell culture apparatus using same |
| CN210438747U (en) * | 2019-07-18 | 2020-05-01 | 中科细胞科技(广州)有限公司 | Liquid supplementing device for cell culture |
| CN111172032A (en) * | 2020-02-19 | 2020-05-19 | 海口健康岛生物科技有限公司 | Automatic incubator for living cells |
| CN212174969U (en) * | 2020-03-17 | 2020-12-18 | 南京诺尔曼生物技术有限公司 | Constant temperature incubator is used in biological cell culture |
| CN112457981A (en) * | 2020-11-27 | 2021-03-09 | 河南灿方机械设备科技有限公司 | Cell culture device for tissue engineering and use method thereof |
| CN214142372U (en) * | 2020-12-17 | 2021-09-07 | 天津市中奥天元科技发展有限公司 | Liquid supplementing device for cell culture |
| CN113801769A (en) * | 2021-09-18 | 2021-12-17 | 新乡医学院 | Culture apparatus for medical science experiments |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111575149A (en) * | 2020-05-09 | 2020-08-25 | 潘齐辉 | Culture device and culture method for slow-release aerobic strains |
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| Publication number | Publication date |
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| CN114395478B (en) | 2023-09-29 |
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Effective date of registration: 20230904 Address after: 201400, 5th Floor, Building 13, No. 6055 Jinhai Road, Fengxian District, Shanghai Applicant after: Shanghai Meixin Huayu Biotechnology Co.,Ltd. Address before: 231200 room 506, Nancheng chuanggu entrepreneurship Park, 281 Danxia Road, Feixi County Economic Development Zone, Hefei City, Anhui Province Applicant before: Anhui Shanhu Information Technology Co.,Ltd. |
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