CN210438747U

CN210438747U - Liquid supplementing device for cell culture

Info

Publication number: CN210438747U
Application number: CN201921135710.XU
Authority: CN
Inventors: 华佩君; 唐忆琳; 陈巧林
Original assignee: Zhongke Cell Technology Guangzhou Co Ltd
Current assignee: Zhongke Cell Technology Guangzhou Co Ltd
Priority date: 2019-07-18
Filing date: 2019-07-18
Publication date: 2020-05-01
Anticipated expiration: 2029-07-18

Abstract

The utility model relates to the field of biological experiment apparatus, in particular to a liquid supplementing device for cell culture, which comprises a culture bottle and a liquid adding cylinder which is detachably and fixedly connected with the culture bottle, wherein the liquid adding cylinder is communicated with the culture bottle; the liquid feeding cylinder is provided with internal threads, and a piston in threaded connection with the internal threads is arranged in the liquid feeding cylinder. The culture solution is added to the culture bottle through the liquid adding cylinder, the culture solution can be added without opening the culture bottle, and the probability of cell contamination is effectively reduced.

Description

Liquid supplementing device for cell culture

Technical Field

The utility model relates to a biological assay utensil field, more specifically relates to a fluid infusion device that cell culture used.

Background

The growth of cells requires a nutrient environment, and a nutrient medium for maintaining the growth of cells is called a culture medium, which is divided into a culture solution and a culture solid. In the process of culturing cells with a culture medium, the cells consume the culture medium, and the culture flask needs to be opened halfway to add the culture medium into the flask, so that the culture flask is likely to be contaminated when opened, and the longer the culture flask is opened, the more the culture flask is likely to be contaminated.

SUMMERY OF THE UTILITY MODEL

The utility model discloses an overcome the problem that the blake bottle need be opened to add culture solution among the above-mentioned prior art, provide a fluid infusion device that cell culture used, need not to open the blake bottle and can accomplish and add the culture solution.

In order to solve the technical problem, the utility model discloses a technical scheme is: a liquid supplementing device for cell culture comprises a culture bottle and a liquid adding cylinder detachably and fixedly connected with the culture bottle, wherein the liquid adding cylinder is communicated with the culture bottle; the liquid feeding cylinder is provided with an internal thread, and a piston in threaded connection with the internal thread is arranged in the liquid feeding cylinder.

The liquid adding cylinder is used for absorbing a certain amount of culture solution, the principle of the liquid adding cylinder is similar to that of an injection needle cylinder, and the culture solution cannot leak out under the condition of no external force pushing because the liquid adding cylinder is in a vacuum state after absorbing the culture solution. And (3) installing the liquid adding cylinder filled with the culture solution on the culture bottle, and after the liquid adding cylinder is fixedly connected with the culture bottle, enabling the liquid adding cylinder and the culture bottle to be in a communicated state. When liquid needs to be added, the piston is rotated, and because the piston is in threaded connection with the liquid adding cylinder, the piston moves downwards, so that the culture solution is pushed to flow to the culture bottle from the liquid adding cylinder while the sealing performance and the liquid adding accuracy are maintained.

Preferably, the surface of the charging barrel is provided with a scale. The addition of the culture solution can be known through the scales, the addition of the culture solution can be better controlled, and the effect of quantitatively controlling the addition amount is achieved.

Preferably, the culture bottle is provided with an installation groove for installing the liquid feeding cylinder, and the side wall of the installation groove is provided with a limit groove; one end of the liquid feeding cylinder, which is close to the culture bottle, is provided with a limiting block which is installed in a matching way with the limiting groove. The liquid feeding cylinder is rotated, so that the limiting block is rotated into the limiting groove and clamped in the limiting groove. The connection and installation of the liquid adding cylinder and the culture bottle are completed, and the installation is quick.

Preferably, a liquid inlet hole is formed in the mounting groove, and a sealing rubber ring is arranged in the liquid inlet hole; the end face of the liquid adding cylinder, which is close to the culture bottle, is provided with a liquid adding nozzle, and the liquid adding nozzle is matched with the liquid inlet hole. The culture solution flows out from the liquid feeding mouth, flows into the blake bottle through the feed liquor hole in, is equipped with the sealing rubber ring on both complex positions, has guaranteed that the position of intercommunication is in sealed state, has avoided simultaneously that the culture solution from the outflow of liquid feeding section of thick bamboo can not accurately flow into the blake bottle.

Preferably, the cartridge is mounted at an edge location remote from a center point of the flask. The liquid adding cylinder is positioned at the edge of the culture bottle and cannot block the microscope from observing cells.

Preferably, the liquid adding device further comprises a fixing frame for fixing the liquid adding cylinder, wherein the fixing frame comprises a base, a connecting rod arranged on the base and a fixing clamp arranged on the connecting rod. The fixing clamp is used for clamping the liquid feeding cylinder, so that the liquid feeding cylinder is prevented from swinging and separating from the culture bottle when the piston is rotated to feed the culture solution.

Preferably, the fixing clamp comprises a clamping seat, fixing plates arranged at two ends of the clamping seat and clamping plates connected with the fixing plates through elastic pieces. The clamp plate clamps the charging barrel through the elastic force of the elastic piece.

Preferably, the fixing clamp is connected with the connecting rod in a sliding mode, and the fixing clamp can be fixed on the connecting rod through a fastening piece. The height of the fixing clamp is adjusted according to the liquid adding cylinder, and the fixing clamp is fixed in position with the fastening piece after being at a proper height.

Preferably, one end of the charging barrel, which is far away from the culture bottle, is provided with a flange used for abutting against the fixing clamp. The fixing clamp reduces the pressure of the liquid feeding cylinder on the culture bottle by giving the flange bearing force, and avoids the over-pressure damage of the culture bottle.

Compared with the prior art, the beneficial effects are: the culture solution is added to the culture bottle through the liquid adding cylinder, the culture solution can be added without opening the culture bottle, and the probability of cell contamination is effectively reduced.

Drawings

FIG. 1 is a perspective view of a fluid infusion device for cell culture according to the present invention.

FIG. 2 is a left side view of the fluid infusion device for cell culture according to the present invention;

FIG. 3 is a schematic structural view of the culture flask of the present invention;

fig. 4 is a schematic structural view of the liquid adding cylinder of the present invention.

Wherein: 1. a culture bottle; 101. mounting grooves; 102. a limiting groove; 103. a liquid inlet hole; 104. sealing the rubber ring; 105. a ventilation cover; 2. a liquid adding cylinder; 201. a limiting block; 202. a liquid adding nozzle; 203. a flange; 3. a piston; 4. a fixed mount; 5. a base; 6. a connecting rod; 7. a fixing clip; 701. a holder; 702. a fixing plate; 703. an elastic member; 704. a splint; 705. a threaded bore.

Detailed Description

The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationships depicted in the drawings are for illustrative purposes only and are not to be construed as limiting the present patent.

The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if there are the terms "upper", "lower", "left", "right", "long", "short", etc. indicating the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore the terms describing the positional relationship in the drawings are only for illustrative purposes and are not to be construed as limiting the present patent, and those skilled in the art will understand the specific meaning of the terms according to their specific circumstances.

The technical solution of the present invention is further described in detail by the following specific embodiments in combination with the accompanying drawings:

examples

As shown in FIGS. 1-4, an embodiment of a fluid infusion device for cell culture comprises a culture bottle 1, a fluid infusion cylinder 2 detachably and fixedly connected with the culture bottle 1, wherein the fluid infusion cylinder 2 is communicated with the culture bottle 1; the liquid feeding cylinder 2 is provided with internal threads, and a piston 3 in threaded connection with the internal threads is arranged in the liquid feeding cylinder 2; the outer surface of the charging barrel 1 is provided with scales. The flask is provided with a gas permeable cap 105.

Specifically, the culture bottle 1 is provided with a mounting groove 101 for the installation of the liquid feeding cylinder 2, the side wall of the mounting groove 101 is provided with a limiting groove 102, the bottom of the mounting groove 101 is provided with a liquid inlet hole 103, and a sealing rubber ring 104 is arranged in the liquid inlet hole 103. One end of the liquid adding cylinder 2 close to the culture bottle 1 is provided with a limiting block 201 extending into the limiting groove 102, the end face close to the culture bottle 1 is provided with a liquid adding nozzle 202, and one end far away from the culture bottle 1 is provided with a flange 203. Aim at feed liquor hole 103 with liquid feeding mouth 202, pack into mounting groove 101 with liquid feeding barrel 2 in, rotate liquid feeding barrel 2 for stopper 201 has accomplished the connection installation of liquid feeding barrel and blake bottle in shifting over to spacing groove 202, and the installation is quick. When liquid is added, the culture solution flows out from the liquid adding nozzle and flows into the culture bottle through the liquid inlet hole, the sealing rubber ring is arranged on the matched position of the liquid inlet nozzle and the liquid inlet hole, the communicated position is in a sealed state, and meanwhile, the phenomenon that the culture solution flowing out from the liquid adding cylinder cannot accurately flow into the culture bottle is avoided.

Wherein, the charging barrel 2 is arranged at the edge of the culture bottle 1. The liquid adding cylinder is positioned at the edge of the culture bottle and cannot block the microscope from observing cells.

In addition, the liquid supplementing device further comprises a fixing frame 4 for fixing the liquid adding barrel, and the fixing frame 4 comprises a base 5, a connecting rod 6 installed on the base 5 and a fixing clamp 7 installed on the connecting rod 6. The fixing clamp is used for clamping the liquid feeding cylinder, so that the liquid feeding cylinder is prevented from swinging and separating from the culture bottle when the piston is rotated to feed the culture solution.

Specifically, the fixing clip 7 includes a holder 701, a fixing plate 702 disposed at two ends of the holder 701, and a clamping plate 704 connected to the fixing plate 702 through an elastic member 703. The clamp plate clamps the charging barrel through the elastic force of the elastic piece. The clamping seat 701 is provided with a threaded hole 705, the clamping seat 701 moves on the connecting rod 6, and when the fixing is needed, the clamping seat 701 is installed in the clamping seat 701 through a fastener, so that the clamping seat 701 is pressed on the connecting rod 6.

After the clamping plate 704 clamps the charging tube 2, the lower end surface of the flange 203 of the charging tube 2 is abutted to the upper end surface of the clamping plate 704, and the clamping plate 704 provides a bearing force for the flange 203, so that the pressure of the charging tube 2 on the culture bottle 1 is reduced, and the culture bottle 1 is prevented from being damaged due to excessive pressure.

The utility model discloses a theory of operation or work flow: the culture bottle 1 is placed on the bottom plate of the fixing frame 4, a certain culture solution is extracted by using the liquid adding cylinder 2, the liquid adding cylinder 2 is installed on the culture bottle 1, and the liquid adding nozzle 201 extends into the liquid inlet hole 103 at the moment. The height of the holder 701 is adjusted and then fixed. The clamping plate 704 is manually opened so that the clamping plate 704 clamps the cartridge 2. When the culture bottle needs to be filled with liquid, the piston 3 is rotated, the culture liquid in the liquid adding cylinder 2 is pressed out, flows out through the liquid adding nozzle 201, and flows into the culture bottle from the liquid inlet hole 103.

The utility model has the advantages that: the culture solution is added to the culture bottle through the liquid adding cylinder, the culture solution can be added without opening the culture bottle, and the probability of cell contamination is effectively reduced.

It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations to the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A liquid supplementing device for cell culture comprises a culture bottle (1) and is characterized by further comprising a liquid adding cylinder (2) detachably and fixedly connected with the culture bottle (1), wherein the liquid adding cylinder (2) is communicated with the culture bottle (1); the liquid adding cylinder (2) is provided with an internal thread, and a piston (3) in threaded connection with the internal thread is arranged in the liquid adding cylinder (2).

2. The fluid infusion device for cell culture according to claim 1, wherein the surface of the infusion cylinder (2) is provided with a scale.

3. The fluid infusion device for cell culture according to claim 1, wherein the culture bottle (1) is provided with a mounting groove (101) for mounting the fluid infusion cylinder (2), and the side wall of the mounting groove (101) is provided with a limiting groove (102); one end of the liquid adding cylinder (2) close to the culture bottle (1) is provided with a limiting block (201) which is matched with the limiting groove (102).

4. The fluid infusion device for cell culture according to claim 3, wherein a fluid inlet hole (103) is formed in the mounting groove (101), and a sealing rubber ring (104) is formed in the fluid inlet hole (103); the end face, close to the culture bottle (1), of the liquid adding cylinder (2) is provided with a liquid adding nozzle (202), and the liquid adding nozzle (202) is matched with the liquid inlet hole (103).

5. The fluid infusion device for cell culture according to claim 1, wherein the fluid infusion cylinder (2) is installed at an edge position away from the center point of the culture flask (1).

6. The fluid infusion device for cell culture according to any one of claims 1 to 5, further comprising a fixing frame (4) for fixing the fluid infusion cylinder (2), wherein the fixing frame (4) comprises a base (5), a connecting rod (6) mounted on the base (5) and a fixing clamp (7) mounted on the connecting rod (6).

7. The fluid infusion device for cell culture according to claim 6, wherein the fixing clip (7) comprises a holder (701), fixing plates (702) disposed at two ends of the holder (701), and a clamping plate (704) connected to the fixing plates (702) through an elastic member (703).

8. The fluid infusion device for cell culture according to claim 6, wherein the fixing clip (7) is slidably connected with the connecting rod (6), and the fixing clip (7) can be fixed on the connecting rod (6) through a fastener.

9. The fluid infusion device for cell culture according to claim 6, wherein a flange (203) for abutting against the fixing clip (7) is provided at one end of the infusion cylinder (2) away from the culture flask (1).