CN212610690U - Cell culture bag - Google Patents

Abstract

The utility model relates to the technical field of cell culture devices, in particular to a cell culture bag, which comprises a sealed bag body; the upper end and the lower end of the sealed bag body are respectively provided with a liquid inlet and a liquid taking port, and a liquid inlet supporting pipe fitting and a liquid taking supporting pipe fitting are respectively arranged in the liquid inlet and the liquid taking port; one end of the liquid inlet supporting pipe fitting, which is far away from the liquid inlet, is communicated with the liquid inlet pipe, and one end of the liquid taking supporting pipe fitting, which is far away from the liquid taking port, is communicated with the liquid taking pipe; the one end that the feed liquor pipe kept away from the feed liquor and supported the pipe fitting is provided with two at least reposition of redundant personnel feed liquor pipes, and the reposition of redundant personnel feed liquor pipe is kept away from the one end of feed liquor pipe and is provided with connecting portion. The utility model discloses set up the reposition of redundant personnel feed liquor pipe rather than the intercommunication on the feed liquor pipe to set up the sealed puncture head rather than detachable connection on reposition of redundant personnel feed liquor pipe, made things convenient for the change of sealed puncture head, and set up a plurality of puncture channels in the inside of sealed puncture head, the leakproofness is good, has avoided original plastic plug to puncture many times and has produced the emergence of damaged seepage scheduling problem.

Description

Cell culture bag

Technical Field

The utility model relates to a cell culture device technical field specifically is a cell culture bag.

Background

The cell culture technology is also called as cell cloning technology, and can maintain the survival of cells through cell culture, realize cell proliferation and obtain cell metabolites after the cell proliferation. Cell culture is widely used in various fields such as biology, medicine, research and development of new drugs, and becomes the most basic and core technology in biotechnology.

The culture device commonly used for cell culture comprises a cell culture dish and a cell culture bottle. However, in the process of large-scale cell culture (such as amplifying immune cells for adoptive immunotherapy, amplifying cell packaging viruses in large scale and amplifying cells in large scale to obtain cell metabolites), the conventional cell culture dish and cell culture bottle have large opening of bottle mouth and poor sealing property. When the amount of the culture medium in the culture container is large, the culture medium is liable to overflow, and the probability of cell contamination increases. Moreover, the cell culture dish and the cell culture bottle need to occupy a large storage space. Particularly for the culture of suspension cells, the requirement on the air exchange rate in a culture vessel is high, and the problems of cell aggregation and high local cell density are easily caused by the way of statically placing the suspension cells in a cell culture vessel or a cell culture bottle for culture, so that the air exchange is not facilitated, and the cell proliferation quantity is reduced.

At present, a cell culture bag made of a breathable liquid-tight polymer material exists, the exchange between the cell culture bag and the outside air can be increased by using the bag body, and the sealing performance is good. However, the conventional cell culture bag for clinical cell culture has a simple structure, the main joint is a plastic plug, the sealing performance is poor, multiple punctures can cause damage and leakage, the requirement of injecting culture solution for multiple times cannot be met, and a cell sample is easily polluted.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell culture bag to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a cell culture bag comprises a sealed bag body; the upper end and the lower end of the sealed bag body are respectively provided with a liquid inlet and a liquid taking port, and a liquid inlet supporting pipe fitting and a liquid taking supporting pipe fitting are respectively arranged in the liquid inlet and the liquid taking port; one end of the liquid inlet supporting pipe fitting, which is far away from the liquid inlet, is communicated with the liquid inlet pipe, and one end of the liquid taking supporting pipe fitting, which is far away from the liquid taking port, is communicated with the liquid taking pipe; at least two shunting liquid inlet pipes are arranged at one ends of the liquid inlet pipes, which are far away from the liquid inlet supporting pipe fitting, connecting parts are arranged at one ends of the shunting liquid inlet pipes, which are far away from the liquid inlet pipes, and mounting grooves are formed in the connecting parts; a sealing puncture head matched with the radian of the mounting groove is clamped in the connecting part; the bottom of sealed puncture head is provided with the feed liquor plunger with reposition of redundant personnel feed liquor pipe radian adaptation, and the inside of sealed puncture head and the inside of feed liquor plunger all are provided with the puncture pipeline of intercommunication each other.

Preferably, the upper surface of the sealed puncture head is provided with a puncture hole corresponding to the puncture channel.

Preferably, the top end of the sealed puncture head is in threaded connection with a dustproof cover.

Preferably, the shunting liquid inlet pipe and the liquid taking pipe are both provided with sealing clamps.

Preferably, at least two opposite connecting convex columns are arranged on the bottom wall of the mounting groove, and a connecting column groove used for being clamped with the connecting convex column and matched with the radian of the connecting convex column is formed in one end, close to the connecting portion, of the sealing puncture head.

Preferably, the left side and the right side of the sealing bag body are both provided with sealing side edges, and the upper end and the lower end of each sealing side edge are both provided with hanging holes for hanging the sealing bag body.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model discloses set up the reposition of redundant personnel feed liquor pipe rather than the intercommunication on the feed liquor pipe to set up the sealed puncture head rather than detachable connection on reposition of redundant personnel feed liquor pipe, very big having made things convenient for the change of sealed puncture head, and set up a plurality of puncture channels in the inside of sealed puncture head, the leakproofness is good, avoided original plastic plug puncture to produce the emergence of damaged seepage scheduling problem many times, can satisfy the needs of pouring into the culture solution many times, cell sample is difficult for being contaminated.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is a schematic structural diagram of part a of the present invention;

FIG. 3 is a schematic view of the connection structure of the sealing puncture head and the split-flow liquid inlet pipe of the present invention;

fig. 4 is a schematic view of the sealing puncture head of the present invention.

In the figure: 1. sealing the bag body; 2. liquid feeding support pipe fittings; 3. taking liquid to support the pipe fitting; 4. a liquid inlet pipe; 5. a liquid taking pipe; 6. a liquid inlet pipe is divided; 7. a connecting portion; 8. installing a groove; 9. sealing the puncture head; 10. a liquid inlet plunger; 11. puncturing a pipeline; 12. a dust cover; 13. sealing and clamping; 14. connecting the convex columns; 15. connecting the column grooves; 16. sealing the side edges; 17. hanging holes; 18. and (6) puncturing the hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Referring to fig. 1-4, the present invention provides a technical solution: the utility model provides a cell culture bag, for realizing above-mentioned purpose, the utility model provides a following technical scheme: a cell culture bag comprises a sealed bag body 1; a liquid inlet and a liquid taking port are respectively arranged at the upper end and the lower end of the sealed bag body 1, and a liquid inlet supporting pipe 2 and a liquid taking supporting pipe 3 are respectively arranged in the liquid inlet and the liquid taking port; one end of the liquid inlet supporting pipe fitting 2, which is far away from the liquid inlet, is communicated with a liquid inlet pipe 4, and one end of the liquid taking supporting pipe fitting 3, which is far away from the liquid taking port, is communicated with a liquid taking pipe 5; at least two shunt liquid inlet pipes 6 are arranged at one ends of the liquid inlet pipes 4 far away from the liquid inlet supporting pipe 2, connecting parts 7 are arranged at one ends of the shunt liquid inlet pipes 6 far away from the liquid inlet pipes 4, and mounting grooves 8 are formed in the connecting parts 7; a sealing puncture head 9 matched with the radian of the mounting groove 8 is clamped in the connecting part 7; the bottom of sealed puncture head 9 is provided with the feed liquor plunger 10 with the adaptation of reposition of redundant personnel feed liquor pipe 6 radians, and the inside of sealed puncture head 9 and the inside of feed liquor plunger 10 all are provided with the puncture pipeline 11 of mutual intercommunication.

Furthermore, a puncture hole 18 corresponding to the puncture tube 11 is provided on the upper surface of the sealed puncture head 9.

Further, the top end of the sealed puncture head 9 is connected with a dust cover 12 through threads.

Furthermore, the shunting liquid inlet pipe 6 and the liquid taking pipe 5 are both provided with a sealing clamp 13.

Furthermore, at least two opposite connecting convex columns 14 are arranged on the bottom wall of the mounting groove 8, and a connecting column groove 15 which is used for being clamped with the connecting convex column 14 and matched with the radian of the connecting convex column 14 is arranged at one end, close to the connecting part 7, of the sealing puncture head 9.

Further, the left side and the right side of the sealed bag body 1 are both provided with a sealed side edge 16, and the upper end and the lower end of the sealed side edge 16 are both provided with hanging holes 17 for hanging the sealed bag body 1.

The working principle is as follows: the utility model discloses before the use, whether operating personnel examine its inner structure earlier is intact, the check is the back of mistake, operating personnel inserts the connecting portion 7 on the reposition of redundant personnel feed liquor pipe 6 tops that corresponds with the bottom that will have the sealed puncture head 9 of shield 12 according to self demand, make the feed liquor plunger 10 of sealed puncture head 9 bottom imbed the inside of reposition of redundant personnel feed liquor pipe 6, and simultaneously, in connecting column groove 15 on the sealed puncture head 9 lower surface of connecting portion 7 diapire connecting projection 14 embedding, accomplish the installation to sealed puncture head 9.

When the cell culture bag is used, an operator firstly rotates the dustproof cover 12 on the corresponding sealed puncture head 9 by hand to take the dustproof cover down, then inserts the corresponding puncture hole 18 on the liquid inlet tip sealed puncture head 9 into the puncture pipeline 11 inside the sealed puncture head 9 and the liquid inlet plunger 10 by using an injector or a liquid inlet device for injecting a culture solution, opens the sealing clamp 13 on the corresponding shunting liquid inlet pipe 6 by hand, injects the culture solution into the sealed bag body 1 through the shunting liquid inlet pipe 6 and the liquid inlet pipe 5, clamps the shunting liquid inlet pipe 6 by using the sealing clamp 13 again, covers the dustproof cover 12 on the sealed puncture head 9, and finishes the injection work of the culture solution; the operator can remove the sealing clip 13 from the liquid extraction tube 5 at the bottom of the sealed bag 1, and extract the cell sap and cells accumulated at the bottom of the sealed bag 1 through the liquid extraction tube 5.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. A cell culture bag, characterized in that: comprises a sealed bag body (1); a liquid inlet and a liquid taking port are respectively arranged at the upper end and the lower end of the sealed bag body (1), and a liquid inlet supporting pipe (2) and a liquid taking supporting pipe (3) are respectively arranged in the liquid inlet and the liquid taking port; one end of the liquid inlet supporting pipe fitting (2) far away from the liquid inlet is communicated with a liquid inlet pipe (4), and one end of the liquid taking supporting pipe fitting (3) far away from the liquid taking port is communicated with a liquid taking pipe (5); at least two shunting liquid inlet pipes (6) are arranged at one ends of the liquid inlet pipes (4) far away from the liquid inlet supporting pipe fitting (2), connecting parts (7) are arranged at one ends of the shunting liquid inlet pipes (6) far away from the liquid inlet pipes (4), and mounting grooves (8) are formed in the connecting parts (7); a sealing puncture head (9) matched with the radian of the mounting groove (8) is clamped in the connecting part (7); the bottom of the sealed puncture head (9) is provided with a liquid inlet plunger (10) matched with the radian of the split-flow liquid inlet pipe (6), and puncture pipelines (11) communicated with each other are arranged inside the sealed puncture head (9) and inside the liquid inlet plunger (10).

2. A cell culture bag according to claim 1, wherein: the upper surface of the sealed puncture head (9) is provided with a puncture hole (18) corresponding to the puncture pipeline (11).

3. A cell culture bag according to claim 1, wherein: the top end of the sealed puncture head (9) is in threaded connection with a dustproof cover (12).

4. A cell culture bag according to claim 1, wherein: and the shunting liquid inlet pipe (6) and the liquid taking pipe (5) are both provided with sealing clamps (13).

5. A cell culture bag according to claim 1, wherein: the bottom wall of the mounting groove (8) is at least provided with two opposite connecting convex columns (14), and one end, close to the connecting part (7), of the sealing puncture head (9) is provided with a connecting column groove (15) which is used for being clamped with the connecting convex columns (14) and matched with the radian of the connecting convex columns (14).

6. A cell culture bag according to claim 1, wherein: the left side and the right side of the sealed bag body (1) are both provided with sealed sides (16), and the upper end and the lower end of each sealed side (16) are both provided with hanging holes (17) for hanging the sealed bag body (1).

Images