CN206956035U - A kind of cell fluid transferring device - Google Patents

A kind of cell fluid transferring device Download PDF

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Publication number
CN206956035U
CN206956035U CN201720492317.0U CN201720492317U CN206956035U CN 206956035 U CN206956035 U CN 206956035U CN 201720492317 U CN201720492317 U CN 201720492317U CN 206956035 U CN206956035 U CN 206956035U
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CN
China
Prior art keywords
bottle
liquid
feed liquor
silicone tube
utility
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201720492317.0U
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Chinese (zh)
Inventor
徐学杰
汪滢
李鹏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHANGHAI KANDA BIOTECHNOLOGY CO Ltd
Original Assignee
SHANGHAI KANDA BIOTECHNOLOGY CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHANGHAI KANDA BIOTECHNOLOGY CO Ltd filed Critical SHANGHAI KANDA BIOTECHNOLOGY CO Ltd
Priority to CN201720492317.0U priority Critical patent/CN206956035U/en
Application granted granted Critical
Publication of CN206956035U publication Critical patent/CN206956035U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

A kind of cell fluid transferring device, including transfer bottle and adapter are the utility model is related to, the transfer bottle has a feed liquor bottleneck and some bottle with liquid-out mouths.The beneficial effects of the utility model are:In use, the bottle with liquid-out mouth of cell liquid transfer bottle is flowed out by silicone tube again after being punctured by the epiconus nematophore of adapter, feed liquor is then opened from feed liquor bottleneck and carries out sterile working, in the absence of the internal pad and internal silicone tube being fixed on bottle cap, and then the risk of germ contamination is greatly reduced, at utmost ensure the success rate of cell culture process;Within the access times of every transfer bottle are limited to three times, equally reduce opportunities for contamination.

Description

A kind of cell fluid transferring device
Technical field
It the utility model is related to biological equipment technical field, more particularly to a kind of cell fluid transferring device.
Background technology
The technological requirement of cell culture technology is stricter, and its main distinction mainly with the work of other common laboratories exists In requiring to keep complete sterile working, the influence of microorganism and other extraneous adverse factors is avoided.Cell culture technology is prevented Only pollution behave is to determine the key of cell culture success or failure, even well-appointed laboratory, if experimenter is careless, behaviour It is lack of standardization to make technology, also results in pollution, such as:Cell needs the cell culture fluid in shaking flask being transferred in incubation In seed transfer bottle, existing single port seed shifts bottle, and internal washer easily drops or bottle interior silicon during bottle cap at the top of opening Sebific duct easily touches when taking out and carries disease germs object and germ contamination occurs, and this can reduce the success rate of cell culture process.In addition, Existing single port seed transfer bottle increases with access times, can undoubtedly increase the possibility that bacterium is retained in bottle.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of cell fluid transferring device, of the prior art to solve Deficiency.
In order to achieve the above object, the purpose of this utility model is achieved through the following technical solutions:
A kind of cell fluid transferring device, including transfer bottle and adapter are provided, the transfer bottle has a feed liquor bottleneck With some bottle with liquid-out mouths, the feed liquor bottleneck is provided with the first bottle cap, is covered with the first sealer on the bottle with liquid-out mouth and is provided with External screw thread and the second bottle cap, the adapter includes connector, at least a silicone tube, two pass joints and C-Flex pipes, described One end of connector is lid, and the opening of the lid is covered with the second sealer, and the lid inner top is provided with described The epiconus nematophore of the hollow structure of second sealer, the side of the epiconus nematophore are provided with some first through hole, the circular cone The bottom surface of spine body is provided with some second through holes, and one end of the silicone tube is tightly connected with second through hole, the lid The internal thread being adapted with the external screw thread with the bottle with liquid-out mouth, at least one in the silicone tube connects by described two Head connects the C-Flex and managed, and the end of the end of the silicone tube or the C-Flex pipes is provided with sterilizing filter.
Above-mentioned cell fluid transferring device, wherein, the bottle with liquid-out mouth has three and set side by side successively with the feed liquor bottleneck Put.
Above-mentioned cell fluid transferring device, wherein, the transfer bottle is PE materials.
Compared with the prior art, the beneficial effects of the utility model are:
In use, the bottle with liquid-out mouth of cell liquid transfer bottle passes through again after being punctured by the epiconus nematophore of adapter Silicone tube flows out, and feed liquor is then opened from feed liquor bottleneck and carries out sterile working, in the absence of the internal pad being fixed on bottle cap and Internal silicone tube, and then the risk of germ contamination is greatly reduced, at utmost ensure the success rate of cell culture process;Every Within the access times of transfer bottle are limited to three times, equally reduce opportunities for contamination.
Brief description of the drawings
Form a part of accompanying drawing of the present utility model to be used for providing further understanding to of the present utility model, this practicality is new The schematic description and description of type is used to explain the utility model, does not form to improper restriction of the present utility model. In accompanying drawing:
Fig. 1 shows the structural representation of the transfer bottle of the utility model cell fluid transferring device;
Fig. 2 shows the structural representation of the adapter of the utility model cell fluid transferring device.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made The every other embodiment obtained, belong to the scope of the utility model protection.
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the utility model can To be mutually combined.
With reference to shown in figure 1, Fig. 2, the utility model cell fluid transferring device includes transfer bottle 1 and adapter, and transfer bottle 1 has There are a feed liquor bottleneck 2 and some bottle with liquid-out mouths 3, feed liquor bottleneck 2 is provided with the first bottle cap 4, first is covered with bottle with liquid-out mouth 3 Sealer 5 is simultaneously provided with the bottle cap 7 of external screw thread 6 and second, and adapter includes connector, at least a silicone tube 8, two pass joints 9 and C- Flex pipes 10, one end of connector is lid, and the opening of lid is covered with the second sealer 11, and lid inner top is provided with The epiconus nematophore 12 of the hollow structure of two sealers 11, the side of epiconus nematophore 12 are provided with some first through hole 13, epiconus The bottom surface of nematophore 12 is provided with some second through holes, and one end of silicone tube 8 is tightly connected with the second through hole, and lid has and bottle with liquid-out The internal thread 14 that mouthfuls 3 external screw thread 6 is adapted, at least one in silicone tube 8 connects C-Flex pipes 10 by two pass joints 9, The end of silicone tube 8 or the end of C-Flex pipes 10 are provided with sterilizing filter 15.In the technical program, bottle with liquid-out mouth 3 has three And it is arranged side by side successively with feed liquor bottleneck 2.Transfer bottle 1 is PE materials, reduces production and application cost.Beaten during use under super-clean bench Drive the first bottle cap 4 of liquid bottleneck 2 into, cell liquid is injected into transfer bottle 1 using liquid-transfering gun, bottle cap is tightened, tears the second envelope Film 11, connector is threadedly coupled with bottle with liquid-out mouth 3, epiconus nematophore 12 punctures on bottle with liquid-out mouth 3 in rotary course One sealer 5, the C-Flex pipes on C-Flex pipes 10 and reactor to be seeded are subjected to sterile connection by sterile pipeline bonding machine, Then in the cell liquid injection reactor that will be shifted in bottle 1, the inoculation operation of reactor is completed.
From above-described embodiment as can be seen that advantage of the present utility model is:
In use, the bottle with liquid-out mouth of cell liquid transfer bottle passes through again after being punctured by the epiconus nematophore of adapter Silicone tube flows out, and feed liquor is then opened from feed liquor bottleneck and carries out sterile working, in the absence of the internal pad being fixed on bottle cap and Internal silicone tube, and then the risk of germ contamination is greatly reduced, at utmost ensure the success rate of cell culture process;Every Within the access times of transfer bottle are limited to three times, equally reduce opportunities for contamination.
Specific embodiment of the utility model is described in detail above, but the utility model is not restricted to the above The specific embodiment of description, it is intended only as example.To those skilled in the art, any equivalent modifications and substitute also all Among category of the present utility model.Therefore, the impartial conversion made in the case where not departing from spirit and scope of the present utility model And modification, it should all cover in the scope of the utility model.

Claims (3)

1. a kind of cell fluid transferring device, it is characterised in that including transfer bottle and adapter, the transfer bottle has a feed liquor Bottleneck and some bottle with liquid-out mouths, the feed liquor bottleneck are provided with the first bottle cap, the first sealer are covered with simultaneously on the bottle with liquid-out mouth Provided with external screw thread and the second bottle cap, the adapter includes connector, at least a silicone tube, two pass joints and C-Flex pipes, One end of the connector is lid, and the opening of the lid is covered with the second sealer, and the lid inner top is provided with The epiconus nematophore of the hollow structure of second sealer, the side of the epiconus nematophore is provided with some first through hole, described The bottom surface of epiconus nematophore is provided with some second through holes, and one end of the silicone tube is tightly connected with second through hole, described Lid has the internal thread that is adapted with the external screw thread of the bottle with liquid-out mouth, and at least one in the silicone tube passes through described two Pass joint connects the C-Flex and managed, and the end of the end of the silicone tube or the C-Flex pipes is provided with sterilizing filter.
2. cell fluid transferring device as claimed in claim 1, it is characterised in that the bottle with liquid-out mouth have three and with the feed liquor Bottleneck is arranged side by side successively.
3. cell fluid transferring device as claimed in claim 1, it is characterised in that the transfer bottle is PE materials.
CN201720492317.0U 2017-05-05 2017-05-05 A kind of cell fluid transferring device Expired - Fee Related CN206956035U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720492317.0U CN206956035U (en) 2017-05-05 2017-05-05 A kind of cell fluid transferring device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720492317.0U CN206956035U (en) 2017-05-05 2017-05-05 A kind of cell fluid transferring device

Publications (1)

Publication Number Publication Date
CN206956035U true CN206956035U (en) 2018-02-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720492317.0U Expired - Fee Related CN206956035U (en) 2017-05-05 2017-05-05 A kind of cell fluid transferring device

Country Status (1)

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CN (1) CN206956035U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113667601A (en) * 2021-07-28 2021-11-19 江苏谱新生物医药有限公司 Device and method for continuous culture and sampling of cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113667601A (en) * 2021-07-28 2021-11-19 江苏谱新生物医药有限公司 Device and method for continuous culture and sampling of cells

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180202

Termination date: 20210505