CN114395478B - Bottle rotating machine for biological cell culture - Google Patents
Bottle rotating machine for biological cell culture Download PDFInfo
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- CN114395478B CN114395478B CN202210063937.8A CN202210063937A CN114395478B CN 114395478 B CN114395478 B CN 114395478B CN 202210063937 A CN202210063937 A CN 202210063937A CN 114395478 B CN114395478 B CN 114395478B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a bottle rotating machine for biological cell culture, which relates to the technical field of biological cell culture and comprises a bracket and an incubator arranged at the top of the bracket, wherein a driving mechanism for driving the culture bottle to move is rotationally arranged in the incubator, a liquid adding component for providing nutrient solution for the culture bottle is arranged below the inner wall of the incubator, and the liquid adding component is communicated with a liquid storage tank on the bracket. According to the invention, the driving mechanism arranged in the incubator drives the culture bottle to rotate circumferentially and simultaneously drives the culture bottle to move up and down, so that biological cells in the culture bottle and nutrient solution can be mixed circumferentially and also can form turbulent mixing, and the efficiency of absorbing the nutrient solution by the biological cells is improved; meanwhile, the liquid adding component is driven by the driving mechanism, so that automatic and repeated small-amount nutrient solution adding for the culture flask is realized, and the workload of operators is reduced.
Description
Technical Field
The invention relates to the technical field of biological cell culture, in particular to a bottle rotating machine for biological cell culture.
Background
Cell culture is also known as cell cloning technology, a formal biological term being cell culture technology. Cell culture is an essential process for the whole biotechnology, or for one of the biological cloning techniques, and is itself a large-scale cloning of cells.
Most of the existing cell culture is carried out through a culture bottle, the culture bottle needs to be rotated in the existing culture process, cells cultured in the culture bottle can be fully mixed with nutrient solution to absorb nutrition, most of the existing culture bottle only rotates circumferentially, the mixing efficiency of the cells in the culture bottle and the nutrient solution is low, meanwhile, the cell culture needs to be carried out manually and little by little to add the nutrient solution in the culture bottle, and the working strength of operators is high.
Disclosure of Invention
The invention aims to solve the problems of the prior art and provides a bottle rotating machine for biological cell culture.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the rotary bottle machine for biological cell culture comprises a bracket and an incubator arranged at the top of the bracket, wherein a driving mechanism for driving the culture bottle to move is rotationally arranged in the incubator, a liquid adding component for providing nutrient solution for the culture bottle is arranged below the inner wall of the incubator, and the liquid adding component is communicated with the liquid tank on the bracket;
the driving mechanism comprises a plurality of turntables which are rotatably arranged on the inner wall of the rear side of the incubator, fixing plates which extend forwards are arranged at the upper end and the lower end of each turntable, a fixed clamping seat is arranged above the lower fixing plates, a movable clamping seat is arranged below the upper fixing plates, the turntables are rotatably connected with the incubator through rotating shafts, the rotating shafts synchronously rotate through the cooperation of belt wheels and synchronous belts, and any rotating shaft is driven by a motor;
the surface of the rotating shaft is provided with a cam, the cam is positioned at the rear of the rotating disc, a sliding plate in contact with the cam is arranged in the incubator in a vertical sliding manner, the sliding plate is positioned below the cam, a connecting rod is detachably connected to the upper part of one end of the sliding plate far away from the cam, a connecting sleeve is arranged at the top of the connecting rod, and the connecting sleeve is rotationally connected with the outer wall of the culture bottle;
the liquid adding components are arranged below the culture bottles in a one-to-one mode, each liquid adding component comprises a sealing box arranged below the inner wall of the culture box and a piston arranged in the sealing box in a sliding mode, a push rod at the top of each piston is connected with the bottom of each sliding plate in a disassembling mode, a liquid outlet hole is formed in the right side of each sealing box, a liquid outlet one-way valve is embedded in each liquid outlet hole, each liquid outlet hole is communicated with a liquid inlet at the top of each connecting sleeve through a hose, a liquid passing port communicated with each liquid inlet is formed in the surface of each culture bottle, a liquid inlet hole is formed in the rear side of each sealing box, and a liquid inlet one-way valve is embedded in each liquid inlet hole and communicated with the corresponding liquid storage box through a water pipe;
the eccentric position of the cam and the movable clamping seat are arranged in the same direction.
Further, the fixed holder and the movable holder are two, and are symmetrically arranged at the front end and the rear end of the fixed plate, and one sides of the fixed holder and the movable holder, which are close to the culture flask, are both arranged as arc lines.
Further, the movable clamping seat is connected with the upper fixing plate through a reset spring.
Further, a sealing rubber ring matched with the liquid passing port is arranged on the inner wall of the connecting sleeve far away from the liquid inlet.
Further, the slide left and right sides all is provided with the slider, the spout that is used for the slider to slide from top to bottom is seted up to incubator inner wall left and right sides.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the driving mechanism arranged in the incubator drives the culture bottle to rotate circumferentially and simultaneously drives the culture bottle to move up and down, so that biological cells in the culture bottle and nutrient solution can be mixed circumferentially and also can form turbulent mixing, and the efficiency of absorbing the nutrient solution by the biological cells is improved; meanwhile, the liquid adding component is driven by the driving mechanism, so that automatic and repeated small-amount nutrient solution adding for the culture flask is realized, and the workload of operators is reduced.
Drawings
FIG. 1 is a front view of a bottle-turning machine for biological cell culture according to the present invention;
FIG. 2 is a side view of a roller bottle machine for biological cell culture according to the present invention;
FIG. 3 is a motion state diagram of FIG. 2;
FIG. 4 is a schematic view showing the mounting positions of the connecting sleeve and the culture flask in the bottle rotating machine for culturing biological cells;
fig. 5 is a motion state diagram of fig. 4.
In the figure: 1. a bracket; 2. an incubator; 3. a culture bottle; 31. a liquid passing port; 4. a driving mechanism; 41. a turntable; 42. a fixing plate; 43. a fixed clamping seat; 44. a movable clamping seat; 45. a cam; 46. connecting sleeves; 47. a connecting rod; 48. a slide plate; 49. sealing rubber rings; 5. a liquid adding component; 51. a piston; 52. a seal box; 53. a hose; 6. a liquid storage tank.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Referring to fig. 1-5, a bottle rotating machine for biological cell culture comprises a bracket 1 and an incubator 2 arranged at the top of the bracket 1, wherein a driving mechanism 4 for driving a culture bottle 3 to move is rotationally arranged in the incubator 2, a liquid adding component 5 for providing nutrient solution for the culture bottle 3 is arranged below the inner wall of the incubator 2, and the liquid adding component 5 is communicated with a liquid storage tank 6 on the bracket 1;
the driving mechanism 4 comprises a plurality of turntables 41 rotatably arranged on the inner wall of the rear side of the incubator 2, fixing plates 42 extending forwards are arranged at the upper end and the lower end of each turntable 41, a fixed clamping seat 43 is arranged above the lower fixing plate 42, a movable clamping seat 44 is arranged below the upper fixing plate 42, the turntables 41 are rotatably connected with the incubator 2 through rotating shafts, the rotating shafts synchronously rotate through the cooperation of belt wheels and synchronous belts, and any rotating shaft is driven by a motor;
the surface of the rotating shaft is provided with a cam 45, the cam 45 is positioned at the rear of the rotating disc 41, a sliding plate 48 contacted with the cam 45 is arranged in the incubator 2 in a sliding way up and down, the sliding plate 48 is positioned below the cam 45, a connecting rod 47 is detachably connected to the upper part of one end of the sliding plate 48 far away from the cam 45, the top of the connecting rod 47 is provided with a connecting sleeve 46, the connecting sleeve 46 is rotationally connected with the outer wall of the culture flask 3, and in actual selection, the number of the cams 45 can be one or a plurality, and in selection, the sliding plate 48 is driven to stably move as a selection standard;
the liquid adding components 5 are arranged below the culture flask 3 in a one-to-one mode, each liquid adding component 5 comprises a sealing box 52 arranged below the inner wall of the culture flask 2 and a piston 51 arranged in the sealing box 52 in a sliding mode, a push rod at the top of the piston 51 is detachably connected with the bottom of the sliding plate 48, a liquid outlet hole is formed in the right side of the sealing box 52, a liquid outlet one-way valve is embedded in the liquid outlet hole, the liquid outlet hole is communicated with a liquid inlet at the top of the connecting sleeve 46 through a hose 53, a liquid passing port 31 communicated with the liquid inlet is formed in the surface of the culture flask 3, a liquid inlet hole is formed in the rear side of the sealing box 52, a liquid inlet one-way valve is embedded in the liquid inlet hole, and the liquid inlet hole is communicated with the liquid storage flask 6 through a water pipe;
the eccentric position of the cam 45 is arranged in the same direction as the movable clamping seat 44.
Further, the fixed clamping seat 43 and the movable clamping seat 44 are two, and are symmetrically arranged at the front end and the rear end of the fixed plate 42, and one side, close to the culture flask 3, of the fixed clamping seat 43 and the movable clamping seat 44 is an arc line.
Further, the movable holder 44 is connected to the upper fixing plate 42 by a return spring.
Further, a sealing rubber ring 49 matched with the liquid passing port 31 is arranged on the inner wall of the connecting sleeve 46 far away from the liquid inlet.
Further, slide blocks are arranged on the left side and the right side of the slide plate 48, and sliding grooves for the slide blocks to slide up and down are arranged on the left side and the right side of the inner wall of the incubator 2.
Working principle: when in use, firstly, the culture flask 3 containing biological cells slides into the space between the fixed clamping seat 43 and the movable clamping seat 44, at the moment, the movable clamping seat 44 is driven to be close to the fixed clamping seat 43 under the action of the reset spring, so as to hold the culture flask 3 between the fixed clamping seat 43 and the movable clamping seat 44, then, the connecting rod 47 at the bottom of the connecting sleeve 46 on the culture flask 3 is connected with the top of the sliding plate 48, then, the push rod at the top of the piston 51 in the sealing box 52 below the culture flask 3 is connected with the bottom of the sliding plate 48, and meanwhile, the hose 53 at the right side of the sealing box 52 is communicated with the liquid inlet at the top of the connecting sleeve 46;
further, the motor is started to drive the rotating shafts to rotate, at the moment, the plurality of rotating shafts are driven to synchronously rotate under the action of the belt wheels and the synchronous belt, and then the plurality of rotary tables 41 are driven to rotate, so that the culture bottles 3 at the front ends of the rotary tables 41 rotate, biological cells in the culture bottles 3 are circumferentially mixed with nutrient solution, and the efficiency of absorbing the nutrient solution by the biological cells is improved;
the cam 45 on the rotating shaft is driven to rotate while the rotating shaft rotates, when the eccentric position of the cam 45 is contacted with the sliding plate 48, the sliding plate 48 is driven to move downwards, the connecting sleeve 46 is driven by the sliding plate 48 to move downwards through the connecting rod 47, the culture flask 3 is driven to downwards squeeze the reset spring, when the eccentric position of the cam 45 is far away from the sliding plate 48, the culture flask 3 is driven to move close to the fixed clamping seat 43 under the action of the reset spring, and the sliding plate 48 is driven to move upwards through the connecting sleeve 46, so that biological cells in the culture flask 3 and nutrient solution move up and down to form turbulent flow type mixing, and the efficiency of absorbing the nutrient solution by the biological cells is further improved;
wherein, in the process of the downward movement of the sliding plate 48, the sliding plate 48 drives the piston 51 to move downward, and the nutrient solution in the sealing box 52 is extruded, so that the nutrient solution enters the liquid inlet of the connecting sleeve 46 from the hose 53, and along with the rotation of the culture bottle 3, when the liquid passing port 31 on the culture bottle 3 is communicated with the liquid inlet, the nutrient solution in the liquid inlet enters the culture bottle 3 from the liquid passing port 31; during the upward movement of the slide plate 48, the piston 51 is driven to move upward, and nutrient solution in the liquid storage tank 6 is pumped into the sealing box 52 through the water pipe, so that the nutrient solution is automatically added to the culture flask 3.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (5)
1. The rotary bottle machine for biological cell culture is characterized by comprising a bracket (1) and an incubator (2) arranged at the top of the bracket (1), wherein a driving mechanism (4) for driving a culture bottle (3) to move is rotationally arranged in the incubator (2), a liquid adding component (5) for providing nutrient solution for the culture bottle (3) is arranged below the inner wall of the incubator (2), and the liquid adding component (5) is communicated with a liquid storage tank (6) on the bracket (1);
the driving mechanism (4) comprises a plurality of turntables (41) which are rotatably arranged on the inner wall of the rear side of the incubator (2), fixing plates (42) which extend forwards are arranged at the upper end and the lower end of each turntable (41), a fixed clamping seat (43) is arranged above each fixing plate (42), a movable clamping seat (44) is arranged below each fixing plate (42), the turntables (41) are rotatably connected with the incubator (2) through rotating shafts, the rotating shafts synchronously rotate through the cooperation of belt wheels and synchronous belts, and any rotating shaft is driven by a motor;
the surface of the rotating shaft is provided with a cam (45), the cam (45) is positioned at the rear of the rotating disc (41), a sliding plate (48) in contact with the cam (45) is arranged in the incubator (2) in an up-down sliding manner, the sliding plate (48) is positioned below the cam (45), a connecting rod (47) is detachably connected to the upper part of one end, far away from the cam (45), of the sliding plate (48), a connecting sleeve (46) is arranged at the top of the connecting rod (47), and the connecting sleeve (46) is rotationally connected with the outer wall of the culture flask (3);
the liquid adding components (5) are arranged below the culture bottles (3) in a one-to-one correspondence manner, each liquid adding component (5) comprises a sealing box (52) arranged below the inner wall of the culture box (2) and a piston (51) arranged in the sealing box (52) in a sliding manner, a push rod at the top of each piston (51) is detachably connected with the bottom of each sliding plate (48), a liquid outlet hole is formed in the right side of each sealing box (52), a liquid outlet one-way valve is embedded in each liquid outlet hole, each liquid outlet hole is communicated with a liquid inlet at the top of each connecting sleeve (46) through a flexible pipe (53), a liquid passing port (31) communicated with the liquid inlet is formed in the surface of each culture bottle (3), a liquid inlet hole is formed in the rear side of each sealing box (52), and each liquid inlet one-way valve is embedded in each liquid inlet hole and is communicated with the corresponding liquid storage box (6) through a water pipe;
the eccentric position of the cam (45) and the movable clamping seat (44) are arranged in the same direction.
2. The bottle rotating machine for biological cell culture according to claim 1, wherein the fixed clamping seat (43) and the movable clamping seat (44) are two, and are symmetrically arranged at the front end and the rear end of the fixed plate (42), and one side of the fixed clamping seat (43) and one side of the movable clamping seat (44) close to the culture bottle (3) are arc lines.
3. A bottle-turning machine for biological cell culture according to claim 2, characterized in that the mobile clamping seat (44) is connected to the upper fixed plate (42) by means of a return spring.
4. The bottle rotating machine for biological cell culture according to claim 1, wherein a sealing rubber ring (49) matched with the liquid passing port (31) is arranged on the inner wall of the connecting sleeve (46) far away from the liquid inlet.
5. The bottle rotating machine for biological cell culture according to claim 1, wherein sliding blocks are arranged on the left side and the right side of the sliding plate (48), and sliding grooves for sliding up and down are formed on the left side and the right side of the inner wall of the incubator (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210063937.8A CN114395478B (en) | 2022-01-20 | 2022-01-20 | Bottle rotating machine for biological cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210063937.8A CN114395478B (en) | 2022-01-20 | 2022-01-20 | Bottle rotating machine for biological cell culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114395478A CN114395478A (en) | 2022-04-26 |
| CN114395478B true CN114395478B (en) | 2023-09-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210063937.8A Active CN114395478B (en) | 2022-01-20 | 2022-01-20 | Bottle rotating machine for biological cell culture |
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| CN (1) | CN114395478B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111575149B (en) * | 2020-05-09 | 2023-07-28 | 湛江安美生物科技有限公司 | Culture device and culture method for slow-release type aerobic bacteria |
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|---|---|---|---|---|
| CN210438747U (en) * | 2019-07-18 | 2020-05-01 | 中科细胞科技(广州)有限公司 | Liquid supplementing device for cell culture |
| CN111172032A (en) * | 2020-02-19 | 2020-05-19 | 海口健康岛生物科技有限公司 | Automatic incubator for living cells |
| CN212174969U (en) * | 2020-03-17 | 2020-12-18 | 南京诺尔曼生物技术有限公司 | Constant temperature incubator is used in biological cell culture |
| CN112457981A (en) * | 2020-11-27 | 2021-03-09 | 河南灿方机械设备科技有限公司 | Cell culture device for tissue engineering and use method thereof |
| CN214142372U (en) * | 2020-12-17 | 2021-09-07 | 天津市中奥天元科技发展有限公司 | Liquid supplementing device for cell culture |
| CN113801769A (en) * | 2021-09-18 | 2021-12-17 | 新乡医学院 | Culture apparatus for medical science experiments |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1626082B1 (en) * | 2004-08-13 | 2008-01-16 | The Automation Partnership (Cambridge) Limited | Shaking system for cell culture incubator |
| US10287539B2 (en) * | 2009-06-09 | 2019-05-14 | Jtec Corporation | Rotating culture vessel and automatic cell culture apparatus using same |
-
2022
- 2022-01-20 CN CN202210063937.8A patent/CN114395478B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210438747U (en) * | 2019-07-18 | 2020-05-01 | 中科细胞科技(广州)有限公司 | Liquid supplementing device for cell culture |
| CN111172032A (en) * | 2020-02-19 | 2020-05-19 | 海口健康岛生物科技有限公司 | Automatic incubator for living cells |
| CN212174969U (en) * | 2020-03-17 | 2020-12-18 | 南京诺尔曼生物技术有限公司 | Constant temperature incubator is used in biological cell culture |
| CN112457981A (en) * | 2020-11-27 | 2021-03-09 | 河南灿方机械设备科技有限公司 | Cell culture device for tissue engineering and use method thereof |
| CN214142372U (en) * | 2020-12-17 | 2021-09-07 | 天津市中奥天元科技发展有限公司 | Liquid supplementing device for cell culture |
| CN113801769A (en) * | 2021-09-18 | 2021-12-17 | 新乡医学院 | Culture apparatus for medical science experiments |
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| CN114395478A (en) | 2022-04-26 |
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Effective date of registration: 20230904 Address after: 201400, 5th Floor, Building 13, No. 6055 Jinhai Road, Fengxian District, Shanghai Applicant after: Shanghai Meixin Huayu Biotechnology Co.,Ltd. Address before: 231200 room 506, Nancheng chuanggu entrepreneurship Park, 281 Danxia Road, Feixi County Economic Development Zone, Hefei City, Anhui Province Applicant before: Anhui Shanhu Information Technology Co.,Ltd. |
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