CN214142372U

CN214142372U - Liquid supplementing device for cell culture

Info

Publication number: CN214142372U
Application number: CN202023059123.2U
Authority: CN
Inventors: 张燕; 张金周
Original assignee: Tianjin Zhongao Tianyuan Technology Development Co ltd
Current assignee: Tianjin Zhongao Tianyuan Technology Development Co ltd
Priority date: 2020-12-17
Filing date: 2020-12-17
Publication date: 2021-09-07
Anticipated expiration: 2030-12-17

Abstract

The utility model provides a fluid infusion device that cell culture used belongs to biological assay utensil technical field. The liquid supplementing device for cell culture comprises a culture mechanism, a liquid supplementing mechanism and a sealing mechanism. The culture mechanism comprises a culture bottle, a funnel, an air outlet pipe and a pressure regulating valve, the funnel is communicated with one side of the culture bottle, the air outlet pipe is communicated with one side of the culture bottle, and the pressure regulating valve is connected to the inner wall of the air outlet pipe. The utility model discloses a pipette, change, threaded rod, branch, support, closing plate, liquid feeding section of thick bamboo, second piston, second spring, second layer board, inlet, first spring, first layer board and first piston's effect to be difficult for causing the purpose of cell pollution when having reached supplementary culture solution, need not to open culture apparatus during supplementary culture solution, carry out the fluid infusion to culture apparatus through the fluid infusion device, can effectively reduce the risk of cell pollution, improve cell culture's effect.

Description

Liquid supplementing device for cell culture

Technical Field

The utility model relates to a biological assay utensil field particularly, relates to a fluid infusion device that cell culture used.

Background

The growth of cells requires a nutrient environment, and a nutrient medium for maintaining the growth of cells is called a culture medium, and the culture medium is divided into a culture solution and a culture solid, wherein the culture solution is consumed by the cells in the process of culturing the cells by the culture solution, and the culture solution is added into the culture bottle because the culture bottle needs to be opened halfway, so that the cell is polluted when the culture bottle is opened, and the longer the culture bottle is opened, the more easily the cell is polluted.

Accordingly, those skilled in the art have provided a solution supplementing device for cell culture to solve the problems set forth in the background art.

SUMMERY OF THE UTILITY MODEL

In order to make up for the deficiency, the utility model provides a fluid infusion device that cell culture used aims at improving the problem that easily causes cell pollution when replenishing the culture solution.

The utility model discloses a realize like this:

the utility model provides a fluid infusion device that cell culture used, including cultivateing mechanism, fluid infusion mechanism and sealing mechanism.

The culture mechanism comprises a culture bottle, a funnel, an air outlet pipe and a pressure regulating valve, the funnel is communicated with one side of the culture bottle, the air outlet pipe is communicated with one side of the culture bottle, the pressure regulating valve is connected with the inner wall of the air outlet pipe, the liquid supplementing mechanism comprises a liquid adding cylinder, a one-way circulation component, a liquid absorbing component, a thread component, a rotating component and a liquid absorbing pipe, the liquid adding cylinder is installed at one side of the culture bottle, a liquid inlet is formed at one side of the liquid adding cylinder, the liquid absorbing pipe is installed at the inner wall of the liquid inlet, the one-way circulation component is connected with the inner wall of the liquid adding cylinder, the thread component is installed at the inner wall of the liquid adding cylinder, the rotating component penetrates through the thread component in a threaded manner, the rotating component is rotatably connected with the liquid absorbing component, the liquid absorbing component can move in the liquid adding cylinder, and the funnel is positioned below the one-way circulation component, the sealing mechanism comprises a second spring, a second supporting plate and a second piston, the second spring is arranged in four equal-angle distribution, two ends of the second spring are connected with the liquid adding cylinder and the second supporting plate, the second piston is installed on one side of the second supporting plate, and the second piston is inserted into the liquid inlet.

In the embodiment of the fluid infusion device for cell culture of the present invention, the sealing mechanism further comprises a round bar, the round bar is connected to one side of the second support plate, and the second spring is sleeved on the outer surface of the second support plate.

In the embodiment of the liquid replenishing device for cell culture of the present invention, the inner wall of the liquid adding cylinder is provided with a groove, and the round bar is inserted into the groove.

The utility model discloses an in the fluid infusion device embodiment that cell culture used, one-way circulation subassembly includes first baffle, second baffle, first spring, first layer board, first piston and drain board, first baffle week side install in liquid feeding section of thick bamboo inner wall, first through-hole has been seted up to first baffle internal surface, second baffle week side connect in first through-hole inner wall, first spring both ends with the second baffle with first layer board is connected, first piston install in first layer board one side, the drain board connect in first baffle one side, the funnel is located first through-hole below.

In an embodiment of the fluid infusion apparatus for cell culture of the present invention, the second through hole is formed on the inner surface of the second partition, the first piston is inserted into the second through hole, and the fluid guide plate is a structure recessed from the periphery to the center.

The utility model discloses an in the embodiment of a fluid infusion device that cell culture used, the imbibition subassembly includes closing plate and support, the support mounting in closing plate one side, the shape of support is concave type, the third through-hole has been seted up to the support internal surface, closing plate sliding connection in liquid feeding section of thick bamboo inner wall, the spout has been seted up to closing plate one side.

The utility model discloses a fluid infusion device embodiment that cell culture used, rotating assembly includes threaded rod, branch, fixture block and change, branch slide run through in the third through-hole, the branch both ends with the fixture block with the threaded rod is connected, the change install in threaded rod one end.

The utility model discloses a fluid infusion device embodiment that cell culture used, the shape of fixture block is the ball-type, the spout with the fixture block is laminated mutually, the fixture block can not pass through the third through-hole.

The utility model discloses a fluid infusion device embodiment for cell culture, the screw assembly includes the extension board, extension board week side connect in liquid feeding section of thick bamboo inner wall, the extension board internal surface set up threaded hole, the threaded rod screw thread run through in the screw hole.

The utility model discloses a fluid infusion device embodiment that cell culture used, culture mechanism still includes support and blotter, the standing groove has been seted up to support one side, the blotter install in the standing groove inner wall, the blake bottle set up in blotter one side.

The utility model has the advantages that: the utility model discloses a fluid infusion device for cell culture who obtains through above-mentioned design, when using, people insert the pipette into the culture solution at first, then clockwise rotation swivel, the swivel can drive the threaded rod clockwise rotation, the threaded rod can drive the branch through the screw hole and upwards move, branch and threaded rod can drive the support and upwards move, the support can drive the closing plate and upwards move, at this moment the closing plate below pressure diminishes in the liquid feeding barrel, form the negative pressure, because negative pressure effect, the second through-hole can be blocked to first piston, the second through-hole is not circulated, when the negative pressure is greater than the elasticity of second spring, the negative pressure can drive the second piston to move right through the second layer board, second piston and inlet break away from, along with the closing plate upwards moves, the culture solution can be inhaled in the liquid feeding barrel through the pipette, when the liquid feeding barrel inhales the culture solution of required volume, anticlockwise rotation swivel, the rotating ring can drive the threaded rod to rotate anticlockwise, the threaded rod can drive the supporting rod to move downwards through the threaded hole, the supporting rod and the threaded rod can drive the support to move downwards, the support can drive the sealing plate to move downwards, the volume below the sealing plate in the liquid adding cylinder is reduced, the pressure is increased, the elastic force of the second spring can drive the second piston to move leftwards through the second supporting plate, the second piston blocks the liquid inlet, meanwhile, when the pressure below the liquid adding cylinder is greater than the elastic force of the first spring, the first piston can be driven to move downwards through the first supporting plate, the first piston is separated from the second through hole, a culture medium flows to the funnel through the second through hole and finally enters the culture bottle, the pressure in the culture bottle can be adjusted through the pressure adjusting valve, the culture solution can be supplemented without opening the culture bottle, the probability of cell pollution is effectively reduced, and the aim of not easily causing cell pollution during supplementing the culture solution is fulfilled, need not to open culture apparatus during supplementary culture solution, carry out the fluid infusion to culture apparatus through the fluid infusion device, can effectively reduce the risk of cell pollution, improve cell culture's effect.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic structural view of a solution replenishing apparatus for cell culture according to an embodiment of the present invention;

FIG. 2 is a schematic structural view of a culture mechanism according to an embodiment of the present invention;

fig. 3 is a schematic structural view of a fluid infusion mechanism according to an embodiment of the present invention;

fig. 4 is a schematic structural view of a one-way flow-through assembly according to an embodiment of the present invention;

FIG. 5 is a schematic structural view of a wicking assembly according to an embodiment of the present invention;

fig. 6 is a schematic structural diagram of a thread assembly according to an embodiment of the present invention;

fig. 7 is a schematic structural diagram of a rotating assembly according to an embodiment of the present invention;

fig. 8 is a schematic structural view of a sealing mechanism according to an embodiment of the present invention.

In the figure: 10-a culture mechanism; 110-a support; 120-a cushion; 130-culture flask; 140-a funnel; 150-an outlet pipe; 160-pressure regulating valve; 170-placing grooves; 20-a fluid infusion mechanism; 210-a cartridge; 220-one-way flow-through assembly; 221-a first separator; 222-a second separator; 223-a first spring; 224-a first pallet; 225-a first piston; 226-a liquid guide plate; 227 — a first via; 228-a second via; 230-a wicking assembly; 231-a sealing plate; 232-a scaffold; 233-a third via; 234-a chute; 240-a threaded assembly; 241-support plate; 242-a threaded hole; 250-a rotating assembly; 251-a threaded rod; 252-a strut; 253-a fixture block; 254-swivel; 260-pipette; 270-liquid inlet; 280-grooves; 30-a sealing mechanism; 310-a second spring; 320-a second pallet; 330-a second piston; 340-round bar.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Examples

Referring to fig. 1, the present invention provides a fluid infusion apparatus for cell culture, which includes a culture mechanism 10, a fluid infusion mechanism 20, and a sealing mechanism 30.

Wherein, fluid infusion mechanism 20 fixed connection is on cultivateing mechanism 10, and sealing mechanism 30 fixed connection is at fluid infusion mechanism 20 inner wall, cultivates mechanism 10 and is used for cultivateing the cell, and fluid infusion mechanism 20 is used for replenishing the culture solution in cultivateing mechanism 10, and sealing mechanism 30 is used for plugging up the mouth of fluid infusion, realizes sealedly, is convenient for reach the purpose of fluid infusion.

Referring to fig. 1 and 2, the culture mechanism 10 includes a culture bottle 130, a funnel 140, an air outlet pipe 150 and a pressure regulating valve 160, the funnel 140 is connected to one side of the culture bottle 130, the air outlet pipe 150 is connected to one side of the culture bottle 130, the pressure regulating valve 160 is connected to an inner wall of the air outlet pipe 150, specifically, the pressure regulating valve 160 is fixedly connected to an inner wall of the air outlet pipe 150 by welding, the culture bottle 130 is used for culturing cells, the air outlet pipe 150 and the pressure regulating valve 160 are used for regulating the pressure in the culture bottle 130 to provide a good living and briquetting environment for the cells, and the funnel 140 is convenient for guiding the culture solution into the culture bottle 130.

In some specific embodiments, the culture mechanism 10 further includes a support 110 and a buffer pad 120, a placing groove 170 is formed in one side of the support 110, the buffer pad 120 is installed on an inner wall of the placing groove 170, specifically, the buffer pad 120 is fixedly installed on the inner wall of the placing groove 170 through gluing, the buffer pad 120 is a rubber pad, the culture bottle 130 is disposed on one side of the buffer pad 120, the support 110 is used for supporting the culture bottle 130, and the buffer pad 120 is used for buffering and protecting the culture bottle 130, so that the culture bottle 130 is not easily vibrated and damaged, and cells in the culture bottle 130 are protected.

Referring to fig. 1, 2 and 3, the fluid infusion mechanism 20 includes a fluid charging barrel 210 and a one-way flow assembly 220, imbibition subassembly 230, screw assembly 240, rotating assembly 250 and pipette 260, charging barrel 210 installs in blake bottle 130 one side, it is concrete, charging barrel 210 is through gluing fixed mounting in blake bottle 130 one side, charging barrel 210 one side has seted up inlet 270, pipette 260 installs in the inlet 270 inner wall, it is concrete, pipette 260 is through gluing fixed mounting in inlet 270 inner wall, one-way circulation subassembly 220 connects in charging barrel 210 inner wall, screw assembly 240 installs in charging barrel 210 inner wall, rotating assembly 250 screw thread runs through in screw assembly 240, rotating assembly 250 rotates and connects in imbibition subassembly 230, imbibition subassembly 230 can remove in charging barrel 210, funnel 140 is located one-way circulation subassembly 220 below, pipette 260 is used for leading-in the charging barrel 210 with the culture solution.

Referring to fig. 1, 2, 3, and 8, the sealing mechanism 30 includes a second spring 310, a second support plate 320, and a second piston 330, the second spring 310 is disposed in four equal angular distributions, two ends of the second spring 310 are connected to the charging cylinder 210 and the second support plate 320, specifically, two ends of the second spring 310 are fixedly connected to the charging cylinder 210 and the second support plate 320 by welding, the second piston 330 is mounted on one side of the second support plate 320, specifically, the second piston 330 is fixedly mounted on one side of the second support plate 320 by gluing, the second piston 330 is inserted into the liquid inlet 270, and the elastic force of the second spring 310 is used to drive the second piston 330 to move leftward by the second support plate 320, so that the second piston 330 blocks the charging cylinder 260, and the charging cylinder 210 is not easily communicated with the culture bottle 130, and cells are not easily contaminated.

In some specific embodiments, the sealing mechanism 30 further includes a round rod 340, the round rod 340 is connected to one side of the second supporting plate 320, specifically, the round rod 340 is fixedly connected to one side of the second supporting plate 320 by welding, the second spring 310 is sleeved on the outer surface of the second supporting plate 320, a groove 280 is formed in the inner wall of the charging barrel 210, the round rod 340 is inserted into the groove 280, and the round rod 340 can move in the groove 280 to improve the stability of the second spring 310.

Referring to fig. 3 and 4, the one-way circulation assembly 220 includes a first partition 221, a second partition 222, a first spring 223, a first support plate 224, a first piston 225 and a liquid guide plate 226, wherein the periphery of the first partition 221 is mounted on the inner wall of the liquid adding cylinder 210, specifically, the periphery of the first partition 221 is fixedly mounted on the inner wall of the liquid adding cylinder 210 by welding, a first through hole 227 is formed in the inner surface of the first partition 221, the periphery of the second partition 222 is connected to the inner wall of the first through hole 227, specifically, the periphery of the second partition 222 is fixedly connected to the inner wall of the first through hole 227 by welding, two ends of the first spring 223 are connected to the second partition 222 and the first support plate 224, specifically, two ends of the first spring 223 are fixedly connected to the second partition 222 and the first support plate 224 by welding, the first piston 225 is mounted on one side of the first support plate 224, specifically, the first piston 225 is fixedly mounted on one side of the first support plate 224 by gluing, the liquid guide plate 226 is connected to one side of the first partition 221, specifically, the liquid guide plate 226 is fixedly connected to one side of the first partition 221 by welding, the funnel 140 is located below the first through hole 227, the first spring 223 is used for driving the first piston 225 to move upwards through the first support plate 224, and the liquid guide plate 226 is used for enabling the culture solution to flow from the charging barrel 210 to the first partition 221 and the second partition 222.

In some embodiments, the second partition 222 has a second through hole 228 formed in an inner surface thereof, the first piston 225 is inserted into the second through hole 228, the liquid guide plate 226 is recessed from the periphery to the center, and the first piston 225 is inserted into the second through hole 228 to block the second through hole 228, so that the space between the charging barrel 210 and the culture bottle 130 is in a closed state.

Referring to fig. 3, 5, 6, and 7, the liquid suction assembly 230 includes a sealing plate 231 and a bracket 232, the bracket 232 is installed at one side of the sealing plate 231, specifically, the bracket 232 is fixedly installed at one side of the sealing plate 231 by welding, the sealing plate 231 is a rubber plate, the bracket 232 is concave, the inner surface of the bracket 232 is provided with a third through hole 233, the sealing plate 231 is slidably connected to the inner wall of the liquid adding barrel 210, one side of the sealing plate 231 is provided with a sliding groove 234, the rotating assembly 250 includes a threaded rod 251, a supporting rod 252, a latch 253 and a rotating ring 254, the supporting rod 252 slidably penetrates through the third through hole 233, two ends of the supporting rod 252 are connected to the latch 253 and the threaded rod 251, specifically, two ends of the supporting rod 252 are fixedly connected to the latch 253 and the threaded rod 251 by welding, the rotating ring 254 is installed at one end of the threaded rod 251, specifically, the rotating ring 254 is fixedly installed at one end of the threaded rod 251 by welding, the rotating ring 254 is used for driving the threaded rod 251 to rotate, the supporting rod 252 and the clamping block 253 are used for enabling the support 232 to drive the sealing plate 231 to move up and down while enabling the threaded rod 251 to rotate, and the sealing plate 231 does not rotate, so that the sealing performance between the sealing plate 231 and the inner wall of the charging barrel 210 can be improved, and the service life of the sealing plate 231 can be effectively prolonged.

In some specific embodiments, the latch 253 has a spherical shape, the sliding groove 234 is attached to the latch 253, the latch 253 cannot pass through the third through hole 233, the threaded assembly 240 includes a support plate 241, the periphery of the support plate 241 is connected to the inner wall of the cartridge 210, specifically, the periphery of the support plate 241 is fixedly connected to the inner wall of the cartridge 210 by welding, a threaded hole 242 is formed in the inner surface of the support plate 241, the threaded rod 251 is threaded through the threaded hole 242, and the threaded rod 251 and the threaded hole 242 are used for enabling the strut 252 and the latch 253 to move up and down along with the rotation of the threaded rod 251.

The working principle of the liquid supplementing device for cell culture is as follows: when the culture solution feeding device is used, firstly, a pipette 260 is inserted into a culture solution, then the rotating ring 254 is rotated clockwise, the rotating ring 254 drives the threaded rod 251 to rotate clockwise, the threaded rod 251 drives the supporting rod 252 to move upwards through the threaded hole 242, the supporting rod 252 and the threaded rod 251 drive the bracket 232 to move upwards, the bracket 232 drives the sealing plate 231 to move upwards, the pressure below the sealing plate 231 in the feeding cylinder 210 is reduced to form negative pressure, due to the action of the negative pressure, the first piston 225 blocks the second through hole 228, the second through hole 228 is not communicated, when the negative pressure is larger than the elastic force of the second spring 310, the negative pressure drives the second piston 330 to move rightwards through the second supporting plate 320, the second piston 330 is separated from the liquid inlet 270, along with the upward movement of the sealing plate 231, the culture solution is sucked into the feeding cylinder 210 through the pipette 260, when the feeding cylinder 210 sucks a required amount of the culture solution, the rotating ring 254 rotates anticlockwise, the rotating ring 254 drives the threaded rod 251 to rotate counterclockwise, the threaded rod 251 drives the supporting rod 252 to move downward through the threaded hole 242, the supporting rod 252 and the threaded rod 251 drive the bracket 232 to move downward, the bracket 232 drives the sealing plate 231 to move downward, at this time, the volume below the sealing plate 231 in the charging cylinder 210 becomes smaller, the pressure becomes higher, at this time, the elastic force of the second spring 310 drives the second piston 330 to move leftward through the second supporting plate 320, the second piston 330 blocks the liquid inlet 270, and at the same time, when the pressure below the charging cylinder 210 is greater than the elastic force of the first spring 223, the first piston 225 is driven to move downward through the first supporting plate 224, the first piston 225 is separated from the second through hole 228, the culture medium flows to the funnel 140 through the second through hole 228 and finally enters the culture bottle 130, the pressure regulating valve 160 can regulate the pressure in the culture bottle 130, the culture medium can be replenished without opening the culture bottle 130, thereby effectively reducing the probability of cell contamination, thereby the purpose that is difficult for causing cell pollution when having reached supplementary culture solution, need not to open culture apparatus when supplementing the culture solution, carries out the fluid infusion to culture apparatus through the fluid infusion device, can effectively reduce cell pollution's risk, improves cell culture's effect.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A fluid infusion device for cell culture is characterized by comprising

The culture mechanism (10) comprises a culture bottle (130), a funnel (140), an air outlet pipe (150) and a pressure regulating valve (160), wherein the funnel (140) is communicated with one side of the culture bottle (130), the air outlet pipe (150) is communicated with one side of the culture bottle (130), and the pressure regulating valve (160) is connected to the inner wall of the air outlet pipe (150);

the liquid supplementing mechanism (20), the liquid supplementing mechanism (20) comprises a liquid adding cylinder (210), a one-way circulation component (220), a liquid absorbing component (230), a thread component (240), a rotating component (250) and a liquid absorbing pipe (260), the liquid adding cylinder (210) is arranged at one side of the culture bottle (130), a liquid inlet (270) is arranged at one side of the liquid adding cylinder (210), the pipette (260) is arranged on the inner wall of the liquid inlet (270), the one-way circulation component (220) is connected with the inner wall of the charging barrel (210), the screw thread component (240) is arranged on the inner wall of the charging barrel (210), the rotating component (250) is threaded through the screw thread component (240), said rotating assembly (250) being rotatably connected to said aspirating assembly (230), said aspirating assembly (230) being movable within said cartridge (210), said funnel (140) being located below said one-way flow-through assembly (220);

the sealing mechanism (30) comprises a second spring (310), a second supporting plate (320) and a second piston (330), the second spring (310) is arranged in four equal-angle distribution, two ends of the second spring (310) are connected with the liquid charging barrel (210) and the second supporting plate (320), the second piston (330) is installed on one side of the second supporting plate (320), and the second piston (330) is inserted into the liquid inlet (270).

2. The fluid infusion device for cell culture according to claim 1, wherein the sealing mechanism (30) further comprises a round rod (340), the round rod (340) is connected to one side of the second support plate (320), and the second spring (310) is sleeved on the outer surface of the second support plate (320).

3. The fluid infusion device for cell culture according to claim 2, wherein a groove (280) is formed in the inner wall of the fluid infusion cylinder (210), and the round rod (340) is inserted into the groove (280).

4. The fluid replacement device for cell culture according to claim 1, the one-way circulation assembly (220) comprises a first partition plate (221), a second partition plate (222), a first spring (223), a first supporting plate (224), a first piston (225) and a liquid guide plate (226), the peripheral side of the first clapboard (221) is arranged on the inner wall of the liquid adding barrel (210), the inner surface of the first clapboard (221) is provided with a first through hole (227), the peripheral side of the second partition plate (222) is connected with the inner wall of the first through hole (227), the two ends of the first spring (223) are connected with the second clapboard (222) and the first supporting plate (224), the first piston (225) is installed on one side of the first supporting plate (224), the liquid guide plate (226) is connected to one side of the first partition plate (221), and the funnel (140) is located below the first through hole (227).

5. The fluid infusion device for cell culture according to claim 4, wherein the second partition (222) has a second through hole (228) formed in an inner surface thereof, the first piston (225) is inserted into the second through hole (228), and the fluid guide plate (226) has a structure in which the periphery thereof is recessed toward the center.

6. The fluid infusion device for cell culture according to claim 1, wherein the fluid infusion assembly (230) comprises a sealing plate (231) and a bracket (232), the bracket (232) is mounted on one side of the sealing plate (231), the bracket (232) is concave, the inner surface of the bracket (232) is provided with a third through hole (233), the sealing plate (231) is slidably connected to the inner wall of the infusion cylinder (210), and one side of the sealing plate (231) is provided with a sliding groove (234).

7. The fluid infusion device for cell culture according to claim 6, wherein the rotating assembly (250) comprises a threaded rod (251), a rod (252), a block (253) and a rotating ring (254), the rod (252) is slidably inserted through the third through hole (233), two ends of the rod (252) are connected with the block (253) and the threaded rod (251), and the rotating ring (254) is mounted at one end of the threaded rod (251).

8. The fluid replacement device for cell culture according to claim 7, wherein the fixture block (253) is spherical, the sliding groove (234) is attached to the fixture block (253), and the fixture block (253) cannot pass through the third through hole (233).

9. The fluid infusion device for cell culture according to claim 7, wherein the screw assembly (240) comprises a support plate (241), the periphery of the support plate (241) is connected to the inner wall of the fluid infusion cylinder (210), a threaded hole (242) is formed in the inner surface of the support plate (241), and the threaded rod (251) is threaded through the threaded hole (242).

10. The fluid replacement device for cell culture according to claim 1, wherein the culture mechanism (10) further comprises a support (110) and a buffer pad (120), a placement groove (170) is formed on one side of the support (110), the buffer pad (120) is mounted on the inner wall of the placement groove (170), and the culture bottle (130) is arranged on one side of the buffer pad (120).