CN111575149B - Culture device and culture method for slow-release type aerobic bacteria - Google Patents
Culture device and culture method for slow-release type aerobic bacteria Download PDFInfo
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Abstract
The invention discloses a slow-release aerobic strain culture device and a slow-release aerobic strain culture method, which belong to the technical field of strain culture. Can realize at the in-process of cultivateing, along with the rocking of blake bottle, automatic intermittent type nature adds the culture medium in to the blake bottle, need not to stop the rocking of blake bottle, has saved the manpower, and has improved cultivation efficiency.
Description
Technical Field
The invention relates to the field of strain culture, in particular to a slow-release aerobic strain culture device and a culture method thereof.
Background
The strain is propagation material composed of edible fungi, industrial fungi, agricultural fungus mycelium and growth matrix thereof. The strain is a basic material for microbiological and life science research, and in the medical field, a complete strain is provided for the preparation of diagnostic products, the production of bacterins, the research of microbial pathogenicity, the bacteriostasis test of medicines, the microbiological inspection of medicines and the like. The strains are divided into a mother strain (first-class strain), an original strain (second-class strain) and a cultivated strain (third-class strain) at three stages.
Aerobic bacteria are one kind of strain, and are also called aerobic bacteria and aerobic microorganisms, and are used for growing and breeding in an aerobic environment, and oxidizing the energy-producing metabolic process of organic matters or inorganic matters, and taking molecular oxygen as a final electron acceptor to perform aerobic respiration. Shake flask culture is one of the common methods of aerobic strain culture, in which strain and culture medium are placed in a flask and then the flask is shaken back and forth.
In the shake flask culture process, along with the consumption of the culture medium, the culture medium needs to be added into the culture flask at regular intervals, the shake flask culture in the prior art needs to be stopped, then the culture medium is manually added, the operation is troublesome, and the culture efficiency can be reduced. Therefore, we propose a slow-release aerobic strain culture device and a culture method thereof.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems in the prior art, the invention aims to provide a slow-release aerobic strain culture device and a slow-release aerobic strain culture method, which can automatically and intermittently add culture medium into a culture flask along with shaking of the culture flask in the culture process, does not need to stop shaking of the culture flask, saves manpower and improves culture efficiency.
2. Technical proposal
In order to solve the problems, the invention adopts the following technical scheme.
The utility model provides a culture apparatus of good oxygen bacterial of slowly-releasing, includes slowly-releasing feeding mechanism and blake bottle, the blake bottle includes the body, the top threaded connection of body has assorted bottle lid, fixedly connected with bottle plug on the top inner wall of bottle lid, the inside fixed mounting of bottle lid has two inlet pipes that symmetry set up, the inlet pipe runs through bottle lid, bottle plug and switches on with the body is inside, the top fixedly connected with connecting port of inlet pipe, body back one side fixedly connected with locating lever, slowly-releasing feeding mechanism includes the backup pad, backup pad openly one side fixedly connected with driving motor, fixedly connected with disc on the output of driving motor, disc openly one side fixedly connected with gangbar, driving motor's below fixedly mounted has the pivot, the epaxial rotation of outer wall of pivot is connected with rocker, the gangbar matched with gangbar's gangbar has been seted up at the middle part, and the pole runs through the gangbar and with its sliding connection, the bottom fixedly connected with of rocker is set up to the seat of U-shaped.
The clamping device is characterized in that two symmetrically arranged clamping springs are fixedly arranged on the inner wall of the clamping seat, one ends of the two clamping springs are fixedly connected with clamping blocks which are matched with the bottle body, one side of each clamping block is fixedly connected with a pull rod, and the pull rods penetrate through the outer wall of the clamping block clamping seat and are in sliding connection with the clamping seat.
The utility model discloses a storage device, including the backup pad, the equal fixedly connected with pushing ram of both sides of cassette, backup pad openly one side fixed mounting has the storage base, the storage tank that two symmetries set up has been seted up to the inside of storage base, pushing ram, storage base, storage tank all set up to the circular-arc that uses the pivot as the center, the pushing ram runs through storage tank and fixedly connected with and storage tank assorted pushing piston, the intercommunication has the filling tube on the outer wall in the storage tank outside, the intercommunication has the passage on the outer wall of storage tank inboard, the other end intercommunication of passage has corrugated hose, corrugated hose's other end fixedly connected with and connecting port assorted connector.
Further, the outer wall of the bottle plug is provided with an annular sealing gasket, the sealing gasket is attached to the inner wall of the bottle body, and the sealing gasket can improve the sealing performance between the bottle body and the bottle cap.
Further, the output end of the driving motor is fixedly connected with the circle center of the disc, and the linkage rod is arranged above the circle center of the disc.
Further, the linkage groove runs through the rocker, and the linkage groove sets up in the top of pivot, through driving motor, disc, gangbar, pivot, rocker, linkage groove's setting, starts driving motor, makes the disc drive the gangbar rotate, and the gangbar passes through the linkage groove and drives the rocker and use the pivot to swing as the center pin, and then drives the holder swing back and forth.
Further, the clamp splice is kept away from one side of clamp spring and is offered with body assorted arc groove, be provided with the rubber pad on the inner wall in arc groove, clamp holder, clamp spring, clamp splice, rubber pad, pull rod's setting, the pull rod of pulling both sides makes two clamp splice keep away from and extrudees clamp spring, with the body between the clamp splice, slowly loosen the pull rod and make the clamp splice press from both sides tight body, can fix the blake to the clamp holder, thereby make the horizontal hunting of clamp holder can drive the blake and make a round trip to shake, and then shake the bottle and cultivate, the setting of rubber pad, the frictional force between multiplicable clamp splice and the body prevents the unexpected landing of blake, and has certain cushioning effect, can prevent that clamp spring clamping force is too big, lead to the body impaired.
Further, set up the constant head tank of seting up on the holder inner wall between the clamp splice, constant head tank and locating lever assorted, the setting of locating lever and constant head tank can be convenient for the link fixation between blake bottle and the holder, and can further improve the steadiness of being connected between blake bottle and the holder.
Further, the culture medium is contained in the storage tank, and the liquid level of the culture medium is not higher than the height of one end, communicated with the storage tank, of the guide pipe, so that the culture medium is prevented from running off along the guide pipe when culture is not started.
Further, the top of filling tube is provided with the check valve, the highly be less than the one end of passage and storage tank intercommunication of filling tube and storage tank intercommunication one end, the setting of check valve can be convenient for add the culture medium in to the storage tank through the filling tube, and can prevent that the culture medium from flowing out through the filling tube.
Further, be provided with the observation window on the outer wall of storage tank openly one side, the setting of observation window can be convenient for observe the surplus of the culture medium in the storage tank, and then in time adds the culture medium to the storage tank.
A culture method of a culture device of slow-release aerobic bacteria comprises the following steps:
s1, rotating to open a bottle cap, putting a proper amount of aerobic bacteria and a culture medium into a bottle body, and screwing the bottle cap;
s2, pulling the pull rods on two sides to enable the two clamping blocks to be far away from each other and squeeze the clamping springs, enabling the bottle body to be arranged between the clamping blocks, enabling the positioning rods to be inserted into the positioning grooves, slowly loosening the pull rods, enabling the clamping blocks to clamp the bottle body, and accordingly fixing the culture bottle on the clamping seat;
s3, connecting the connector with a connecting port on the corresponding side to enable the material guiding pipe to be communicated with the interior of the bottle body;
s4, starting a driving motor to drive the culture flask to shake back and forth, and intermittently pushing the culture medium in the storage tank upwards by the pushing piston in the shaking process, wherein when the liquid level of the culture medium is higher than that of the material guiding pipe, the culture medium flows into the culture flask along the material guiding pipe, the corrugated hose and the material feeding pipe, so that the culture medium is automatically and intermittently supplemented into the culture flask;
and S5, in the culture process, a culture medium is added into the storage tank through a one-way valve on the feeding pipe, shaking of the culture bottle is not required to be stopped, after 3-7 days, the culture is completed, the driving motor is closed, and the culture bottle is taken down.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
(1) This scheme is through pushing ram, the storage base, the storage silo, the pushing ram, the filling tube, the check valve, the passage, corrugated hose, the setting of connector, the swing of cassette can make the pushing ram drive the medium in the promotion storage silo of pushing ram intermittent type nature, make the medium liquid level rise, after the liquid level of medium is higher than the passage, the medium can flow along the passage, and then in corrugated hose, the inlet pipe inflow body, thereby automatic intermittent type nature is to the supplementary medium in the blake bottle, the manpower has been saved, and need not to stop the cultivation, and then the cultivation efficiency has been improved.
(2) The sealing gasket can improve the sealing property between the bottle body and the bottle cap.
(3) Through driving motor, disc, gangbar, pivot, rocker, the setting of gangbar groove, start driving motor makes the disc drive the gangbar rotate, and the gangbar passes through the gangbar and drives the rocker and use the pivot to swing as the center pin, and then drives the holder swing back and forth.
(4) The setting of holder, clamping spring, clamp splice, rubber pad, pull rod, the pull rod of pulling both sides makes two clamp splice keep away from and extrudees clamping spring, with the body between the clamp splice, slowly loosen the pull rod and make the clamp splice press from both sides tight body, can fix the blake to the holder on, thereby make the horizontal hunting of holder can drive the blake and make a round trip to shake, and then shake the bottle and cultivate, the setting of rubber pad, the frictional force between clamp splice and body can be increased, prevent the unexpected landing of blake, and have certain cushioning effect, can prevent to press from both sides tight spring clamp force too big, lead to the body impaired.
(5) The setting of locating lever and constant head tank can be convenient for be connected fixedly between blake bottle and cassette, and can further improve the steadiness of being connected between blake bottle and cassette.
(6) The inside of the storage tank is provided with a culture medium, and the liquid level of the culture medium is not higher than the height of one end of the feed guide pipe communicated with the storage tank, so that the culture medium is prevented from running off along the feed guide pipe when culture is not started.
(7) The setting of check valve can be convenient for add the culture medium in to the stock chest through the filling tube, and can prevent that the culture medium from flowing out through the filling tube.
(8) Be provided with the observation window on the outer wall of storage tank openly one side, the setting of observation window can be convenient for observe the surplus of the culture medium in the storage tank, and then in time adds the culture medium to the storage tank.
Drawings
FIG. 1 is a schematic diagram of a culture apparatus for a slow-release aerobic bacterial strain according to the present invention;
FIG. 2 is an enlarged view of the invention at A in FIG. 1;
FIG. 3 is a cross-sectional view of the inside of the body of the present invention;
FIG. 4 is a top view of the present invention at the body;
FIG. 5 is a top view of the holder of the present invention;
FIG. 6 is a cross-sectional view of the present invention within a storage base;
FIG. 7 is a view showing the operation of the apparatus for culturing a slow-release aerobic bacterial strain according to the present invention.
The reference numerals in the figures illustrate:
1. a slow-release feeding mechanism; 101. a support plate; 102. a driving motor; 103. a disc; 104. a linkage rod; 105. a rotating shaft; 106. a rocker; 107. a linkage groove; 108. a clamping seat; 109. a clamping spring; 110. clamping blocks; 111. a rubber pad; 112. a pull rod; 113. a positioning groove; 114. a pushing rod; 115. a storage base; 116. a storage tank; 117. pushing the material piston; 118. a feeding tube; 119. a one-way valve; 120. a material guiding pipe; 121. a corrugated hose; 122. a connector; 123. an observation window; 2. a culture bottle; 201. a bottle body; 202. a bottle cap; 203. a bottle stopper; 204. a feed pipe; 205. a connection port; 206. a sealing gasket; 207. and a positioning rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present invention are within the protection scope of the present invention.
In the description of the present invention, it should be noted that the positional or positional relationship indicated by the terms such as "upper", "lower", "inner", "outer", "top/bottom", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "configured to," "engaged with," "connected to," and the like are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Example 1:
referring to fig. 1 and 3-4, a slow-release aerobic bacteria culture device comprises a slow-release feeding mechanism 1 and a culture flask 2, wherein the culture flask 2 comprises a flask body 201, a matched flask cover 202 is connected with the top end of the flask body 201 in a threaded manner, a bottle stopper 203 is fixedly connected to the inner wall of the top of the flask cover 202, an annular sealing gasket 206 is arranged on the outer wall of the bottle stopper 203, the sealing gasket 206 is attached to the inner wall of the flask body 201, the sealing gasket 206 is arranged to improve the tightness between the flask body 201 and the flask cover 202, two symmetrically arranged feeding pipes 204 are fixedly arranged in the flask cover 202, the feeding pipes 204 penetrate through the flask cover 202 and the flask cover 203 and are communicated with the inside of the flask body 201, a connecting port 205 is fixedly connected to the top end of the feeding pipes 204, a positioning rod 207 is fixedly connected to one side of the back of the flask body 201, the slow-release feeding mechanism 1 comprises a supporting plate 101, a driving motor 102 is fixedly arranged on one side of the front side of the supporting plate 101, the output end of the driving motor 102 is fixedly connected with a disc 103, one side of the front surface of the disc 103 is fixedly connected with a linkage rod 104, a rotating shaft 105 is fixedly arranged below the driving motor 102, the output end of the driving motor 102 is fixedly connected with the circle center of the disc 103, the linkage rod 104 is arranged above the circle center of the disc 103, a rocker 106 is rotationally connected on the outer wall of the rotating shaft 105, a linkage groove 107 matched with the linkage rod 104 is arranged in the middle of the rocker 106, the linkage rod 104 penetrates through the linkage groove 107 and is in sliding connection with the linkage groove 107, the bottom end of the rocker 106 is fixedly connected with a clamping seat 108 which is arranged into a U shape, the linkage groove 107 penetrates through the rocker 106, the linkage groove 107 is arranged above the rotating shaft 105, the driving motor 102, the disc 103, the linkage rod 104, the rotating shaft 105, the rocker 106 and the linkage groove 107 are arranged, the driving motor 102 is started to drive the disc 103 to rotate, the linkage rod 104 drives the rocker 106 to swing back and forth by taking the rotating shaft 105 as a central shaft through the linkage groove 107, and then drives the clamping seat 108 to swing back and forth.
Referring to fig. 1-2 and 5, two symmetrically arranged clamping springs 109 are fixedly arranged on the inner wall of the clamping seat 108, one ends of the two clamping springs 109 are fixedly connected with clamping blocks 110 matched with a bottle body 201, one side of each clamping block 110 is fixedly connected with a pull rod 112, the pull rods 112 penetrate through the outer wall of the clamping seat 108 of the clamping block 110 and are slidably connected with the clamping seat 108, one side of each clamping block 110, far away from the clamping springs 109, is provided with an arc-shaped groove matched with the bottle body 201, the inner wall of each arc-shaped groove is provided with a rubber pad 111, the clamping seat 108, the clamping springs 109, the clamping blocks 110, the rubber pads 111 and the pull rods 112 are arranged, the pull rods 112 on two sides are pulled to enable the two clamping blocks 110 to be far away from and squeeze the clamping springs 109, the bottle body 201 is arranged between the clamping blocks 110, the pull rod 112 is slowly loosened to enable the clamp block 110 to clamp the bottle 201, the culture bottle 2 can be fixed on the clamp seat 108, so that the left and right swing of the clamp seat 108 can drive the culture bottle 2 to swing back and forth, shake bottle culture is further carried out, the rubber pad 111 is arranged, friction force between the clamp block 110 and the bottle 201 can be increased, the culture bottle 2 is prevented from accidentally sliding down, a certain buffer effect is achieved, the clamping force of the clamp spring 109 can be prevented from being excessively large, the bottle 201 is prevented from being damaged, the clamp block 110 is provided with the positioning groove 113 arranged on the inner wall of the clamp seat 108, the positioning groove 113 is matched with the positioning rod 207, the positioning rod 207 and the positioning groove 113 are arranged, the culture bottle 2 can be conveniently connected and fixed with the clamp seat 108, and the connection stability between the culture bottle 2 and the clamp seat 108 can be further improved.
Referring to fig. 1 and 6-7, both sides of the clamping seat 108 are fixedly connected with a pushing rod 114, one side of the front surface of the supporting plate 101 is fixedly provided with a storage base 115, two symmetrically arranged storage tanks 116 are arranged in the storage base 115, the pushing rod 114, the storage base 115 and the storage tanks 116 are all arranged in an arc shape with a rotating shaft 105 as a center, the pushing rod 114 penetrates through the storage tanks 116 and is fixedly connected with a pushing piston 117 matched with the storage tanks 116, the outer wall of the outer side of the storage tanks 116 is communicated with a feeding pipe 118, the outer wall of the inner side of the storage tanks 116 is communicated with a material guide pipe 120, the inside of the storage tanks 116 is filled with culture medium, the liquid level of the culture medium is not higher than the height of one end of the material guide pipe 120 communicated with the storage tanks 116, so that when culture is not started, the culture medium runs off along the material guide pipe 120, the top end of the feeding pipe 118 is provided with a one-way valve 119, the feeding tube 118 and the storage tank 116 are communicated with one end of a lower height than the end of the feeding tube 120 and the storage tank 116, the one-way valve 119 is arranged, the culture medium can be conveniently added into the storage tank 116 through the feeding tube 118, the culture medium can be prevented from flowing out through the feeding tube 118, the other end of the feeding tube 120 is communicated with the corrugated hose 121, the other end of the corrugated hose 121 is fixedly connected with the connector 122 matched with the connecting port 205, the arrangement of the pushing rod 114, the storage base 115, the storage tank 116, the pushing piston 117, the feeding tube 118, the one-way valve 119, the feeding tube 120, the corrugated hose 121 and the connector 122 can enable the pushing rod 114 to drive the pushing piston 117 to intermittently push the culture medium in the storage tank 116 through the back and forth swing of the clamping seat 108, so that the culture medium liquid level rises, when the culture medium liquid level is higher than that of the feeding tube 120, the culture medium can flow out along the feeding tube 120, and further flows into the bottle body 201 along the corrugated hose 121 and the feeding tube 204, thereby automatic intermittent type nature is to the supplementary culture medium in the blake bottle 2, has saved the manpower, and need not to stop the cultivation, and then has improved cultivation efficiency, is provided with observation window 123 on the outer wall of storage silo 116 front one side, and the setting of observation window 123 can be convenient for observe the surplus of the culture medium in the storage silo 116, and then in time adds the culture medium to the storage silo 116.
Referring to FIGS. 1 to 7, a method for culturing a slow-release aerobic strain culture apparatus includes the following steps:
s1, opening the bottle cap 202 in a rotating way, putting a proper amount of aerobic bacteria and a culture medium into the bottle body 201, and screwing the bottle cap 202;
s2, pulling the pull rods 112 on two sides, keeping the two clamping blocks 110 away from each other and pressing the clamping springs 109, placing the flask body 201 between the clamping blocks 110, inserting the positioning rods 207 into the positioning grooves 113, slowly loosening the pull rods 112, and clamping the flask body 201 by the clamping blocks 110, thereby fixing the culture flask 2 on the clamping seat 108;
s3, connecting the connector 122 with the connecting port 205 on the corresponding side to enable the material guiding pipe 120 to be communicated with the interior of the bottle body 201;
s4, starting a driving motor 102 to drive the culture flask 2 to shake back and forth, and in the shaking process, intermittently pushing the culture medium in the storage tank 116 upwards by the pushing piston 117, wherein when the liquid level of the culture medium is higher than that of the material guide pipe 120, the culture medium flows into the culture flask 2 along the material guide pipe 120, the corrugated hose 121 and the material inlet pipe 204, so that the culture medium is automatically and intermittently supplemented into the culture flask 2;
and S5, in the culture process, a culture medium can be added into the storage tank 116 through the one-way valve 119 on the feeding pipe 118, shaking of the culture flask 2 is not required to be stopped, after 3-7 days, the driving motor 102 is closed after the culture is completed, and the culture flask 2 is taken down.
The above description is only of the preferred embodiments of the present invention; the scope of the invention is not limited in this respect. Any person skilled in the art, within the technical scope of the present disclosure, may apply to the present invention, and the technical solution and the improvement thereof are all covered by the protection scope of the present invention.
Claims (10)
1. The utility model provides a culture apparatus of good oxygen bacterial of slowly-releasing, includes slowly-releasing feeding mechanism (1) and blake bottle (2), blake bottle (2) include body (201), the top threaded connection of body (201) has assorted bottle lid (202), fixedly connected with bottle plug (203) on the top inner wall of bottle lid (202), the inside fixed mounting of bottle lid (202) has inlet pipe (204) that two symmetries set up, inlet pipe (204) run through bottle lid (202), bottle plug (203) and are switched on with body (201) inside, the top fixedly connected with connecting port (205) of inlet pipe (204), its characterized in that: the novel slow-release feeding mechanism is characterized in that a positioning rod (207) is fixedly connected to one side of the back of the bottle body (201), the slow-release feeding mechanism (1) comprises a supporting plate (101), a driving motor (102) is fixedly installed on one side of the front of the supporting plate (101), a disc (103) is fixedly connected to the output end of the driving motor (102), a linkage rod (104) is fixedly connected to one side of the front of the disc (103), a rotating shaft (105) is fixedly installed below the driving motor (102), a rocker (106) is rotatably connected to the outer wall of the rotating shaft (105), a linkage groove (107) matched with the linkage rod (104) is formed in the middle of the rocker (106), the linkage rod (104) penetrates through the linkage groove (107) and is in sliding connection with the linkage groove, and a clamping seat (108) which is arranged into a U shape is fixedly connected to the bottom end of the rocker (106).
Two symmetrically arranged clamping springs (109) are fixedly arranged on the inner wall of the clamping seat (108), one ends of the two clamping springs (109) are fixedly connected with clamping blocks (110) matched with the bottle body (201), one side of each clamping block (110) is fixedly connected with a pull rod (112), and each pull rod (112) penetrates through the outer wall of the clamping seat (108) of each clamping block (110) and is in sliding connection with the clamping seat (108);
both sides of cassette (108) are all fixedly connected with pushing ram (114), positive one side fixed mounting of backup pad (101) has storage base (115), storage silo (116) that two symmetries set up have been seted up to the inside of storage base (115), pushing ram (114), storage base (115), storage silo (116) all set up to the circular-arc with pivot (105) as the center, pushing ram (114) run through storage silo (116) and fixedly connected with and storage silo (116) assorted pushing ram (117), the intercommunication has feed tube (118) on the outer wall in storage silo (116) outside, the intercommunication has baffle (120) on the outer wall of storage silo (116) inboard, the other end intercommunication of baffle (120) has bellows (121), the other end fixedly connected with of bellows (121) and connecting port (205) assorted connector (122).
2. The culture device of the slow-release aerobic bacteria according to claim 1, wherein: an annular sealing gasket (206) is arranged on the outer wall of the bottle plug (203), and the sealing gasket (206) is attached to the inner wall of the bottle body (201).
3. The culture device of the slow-release aerobic bacteria according to claim 1, wherein: the output end of the driving motor (102) is fixedly connected with the circle center of the disc (103), and the linkage rod (104) is arranged above the circle center of the disc (103).
4. The culture device of the slow-release aerobic bacteria according to claim 1, wherein: the linkage groove (107) penetrates through the rocker (106), and the linkage groove (107) is arranged above the rotating shaft (105).
5. The culture device of the slow-release aerobic bacteria according to claim 1, wherein: an arc-shaped groove matched with the bottle body (201) is formed in one side, far away from the clamping spring (109), of the clamping block (110), and a rubber pad (111) is arranged on the inner wall of the arc-shaped groove.
6. The culture device of the slow-release aerobic bacteria according to claim 1, wherein: a positioning groove (113) arranged on the inner wall of the clamping seat (108) is arranged between the clamping blocks (110), and the positioning groove (113) is matched with the positioning rod (207).
7. The culture device of the slow-release aerobic bacteria according to claim 1, wherein: the inside of the storage tank (116) is provided with a culture medium, and the liquid level of the culture medium is not higher than the height of one end of the material guide pipe (120) communicated with the storage tank (116).
8. The culture device of the slow-release aerobic bacteria according to claim 1, wherein: the top of charging tube (118) is provided with check valve (119), the height of charging tube (118) and stock chest (116) intercommunication one end is less than the one end that passage (120) and stock chest (116) communicate.
9. The culture device of the slow-release aerobic bacteria according to claim 1, wherein: an observation window (123) is arranged on the outer wall of one side of the front face of the storage groove (116).
10. The culture method of a culture apparatus for a slow-release aerobic bacterial strain according to any one of claims 1 to 9, characterized by: the method comprises the following steps:
s1, rotating to open a bottle cap (202), putting a proper amount of aerobic bacteria and a culture medium into a bottle body (201), and screwing the bottle cap (202);
s2, pulling the pull rods (112) on two sides, keeping the two clamping blocks (110) away from each other and pressing the clamping springs (109), placing the bottle body (201) between the clamping blocks (110), inserting the positioning rods (207) into the positioning grooves (113), slowly loosening the pull rods (112), and clamping the bottle body (201) by the clamping blocks (110), so that the culture bottle (2) is fixed on the clamping seat (108);
s3, connecting the connector (122) with a connecting port (205) on the corresponding side, so that the guide pipe (120) is communicated with the inside of the bottle body (201);
s4, starting a driving motor (102) to drive the culture flask (2) to shake back and forth, and intermittently pushing the culture medium in the storage tank (116) upwards by the pushing piston (117) in the shaking process, wherein when the liquid level of the culture medium is higher than that of the material guide pipe (120), the culture medium flows into the culture flask (2) along the material guide pipe (120), the corrugated hose (121) and the material inlet pipe (204), so that the culture medium is automatically and intermittently supplemented into the culture flask (2);
and S5, in the culture process, a culture medium can be added into the Chu Liaocao (116) through a one-way valve (119) on the feeding pipe (118), shaking of the culture flask (2) is not required to be stopped, and after 3-7 days, the driving motor (102) is closed and the culture flask (2) is taken down.
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| CN110896852A (en) * | 2018-12-18 | 2020-03-24 | 北京林业大学 | Potential energy driven intermittent immersion type bioreactor |
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| CN207362255U (en) * | 2017-10-18 | 2018-05-15 | 上海柏根生物科技有限公司 | A kind of attached cell blake bottle |
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| CN114395478B (en) * | 2022-01-20 | 2023-09-29 | 上海美心华域生物科技有限公司 | Bottle rotating machine for biological cell culture |
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