CN112625909B

CN112625909B - Plant cell culture tank

Info

Publication number: CN112625909B
Application number: CN202011529699.2A
Authority: CN
Inventors: 梁庆开
Original assignee: Guangxi Bagui Seedling Hi Tech Group Co ltd
Current assignee: Guangxi Bagui Seedling Hi Tech Group Co ltd
Priority date: 2020-12-22
Filing date: 2020-12-22
Publication date: 2022-11-22
Anticipated expiration: 2040-12-22
Also published as: CN112625909A

Abstract

The invention discloses a plant cell culture tank which comprises a culture tank body and a motor groove, wherein the inner wall of the motor groove is fixedly connected with a rotating motor, an output shaft of the rotating motor penetrates through the motor groove and the side wall of the culture tank body, the output shaft of the rotating motor is in sealed rotating connection with the connection part of the motor groove and the side wall of the culture tank body, the output shaft of the rotating motor is fixedly connected with a first gear, the side wall of the culture tank body is provided with an annular rack, the inner wall of the lower end of the culture tank body is provided with an annular chute, and a plurality of second gears are arranged between the first gear and the annular rack. The invention can make the culture cylinder revolve around the light source and rotate at the same time, so that all plant cells in the culture cylinder receive uniform illumination, the problem of uneven development of the cultured plant cells caused by the problem of illumination angle is avoided, the culture effect of the plant cells is improved, and the culture rate of the plant cells is increased.

Description

Plant cell culture tank

Technical Field

The invention relates to the technical field of plant cell tissues, in particular to a plant cell culture tank.

Background

The cell culture refers to a method for simulating in vivo environment (sterile, suitable temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow, reproduce and maintain main structures and functions, the cell culture technology can be a simple single cell or a few differentiated multi-cells through mass culture of one cell, the cell culture technology is an important and common technology in cell biology research methods, a large number of cells can be obtained through cell culture, signal transduction of the cells, anabolism of the cells, growth and multiplication of the cells and the like can be researched, the cell culture is divided into animal cell culture and plant cell culture, the plant cell culture is a technology for obtaining a large number of cell populations by placing callus or other easily dispersed tissues in a liquid culture medium for culture under an in vitro condition to obtain dispersed free suspension cells, and the cells are multiplied through subculture, and the technology for obtaining the large number of cell populations is provided, and light and hormone in the plant cell culture are two important elements.

However, in the prior art, the fixed light source is arranged above the culture device for illumination in the plant cell culture process, the plant cells in the culture device are not uniformly developed due to the illumination angle, the plant cells close to the light source are well developed, the plant cells far away from the light source are poor in development and even die, the culture effect is poor, the culture rate of the plant cells is low, and the plant cells and the culture solution are mixed and usually need to be manually operated by an operator regularly, so that the workload of the operator is increased, and the working efficiency is reduced.

To this end, we propose a plant cell culture tank to solve the above problems.

Disclosure of Invention

The invention aims to solve the problems that in the prior art, a fixed light source is arranged above a culture device for illumination in the plant cell culture process, plant cells in the culture device are not uniformly developed due to the illumination angle, the plant cells close to the light source are excellent in development, the plant cells far away from the light source are poor in development and even die, the culture effect is poor, the culture rate of the plant cells is low, and the plant cells and a culture solution are mixed, so that an operator is required to perform manual operation regularly, the work load of the operator is increased, and the work efficiency is reduced.

In order to achieve the purpose, the invention adopts the following technical scheme:

a plant cell culture tank comprises a culture tank body and a motor groove, wherein the inner wall of the motor groove is fixedly connected with a rotating motor, an output shaft of the rotating motor penetrates through the motor groove and the side wall of the culture tank body, the output shaft of the rotating motor is connected with the motor groove and the side wall of the culture tank body in a sealing and rotating manner, the output shaft of the rotating motor is fixedly connected with a first gear, the side wall of the culture tank body is provided with an annular rack, the inner wall of the lower end of the culture tank body is provided with an annular sliding groove, a plurality of second gears are arranged between the first gear and the annular rack, the side wall of the lower end of each second gear is fixedly connected with a sliding block matched with the annular sliding groove, the side wall of the upper end of each second gear is provided with a groove, the side wall of the upper end of the first gear is provided with a rotating groove, a plurality of light sources are arranged in the rotating groove, and the side wall of the upper end of the rotating groove is fixedly connected with a rotating rod, the end of the rotating rod, far away from the rotating groove, is rotatably connected with the inner wall of the culture tank body, the side wall of the rotating rod is fixedly connected with a plurality of sliding grooves, the inner wall of each sliding groove is slidably connected with a special-shaped block, the side walls of the two sides of the special-shaped block are respectively provided with a plurality of clamping grooves, the inner walls of the two sides of each sliding groove are respectively provided with a mounting groove, two sliding rods are fixedly connected between the inner walls of the two mounting grooves, the two sliding rods are sleeved with the same sliding block, the side walls of the two sliding blocks are respectively fixedly connected with a plurality of clamping blocks matched with the clamping grooves, the clamping blocks respectively penetrate through the side walls of the mounting grooves, the joints of the clamping blocks and the side walls of the mounting grooves are hermetically and slidably connected, the side walls of the sliding grooves are respectively and fixedly connected with double-rod hydraulic rods, and the two movable ends of the double-rod hydraulic rods equally divide the side walls of the two mounting grooves, two expansion ends of two pole hydraulic stem respectively with the sealed sliding connection of junction of two mounting groove lateral walls, two expansion ends of two pole hydraulic stem respectively with the lateral wall fixed connection of two sliding blocks, it is a plurality of the equal fixedly connected with bearing in one side of sliding tray is kept away from to dysmorphism piece, it is a plurality of the equal fixedly connected with culture drum of inner wall of bearing, it is a plurality of the equal fixedly connected with of lower extreme lateral wall of culture drum matches with the recess fixed block, it is a plurality of the upper end lateral wall of culture drum is equipped with sealed lid, and is a plurality of the equal fixedly connected with connecting pipe of sealed lid, it is a plurality of the connecting pipe all runs through sealed lid, and is a plurality of the connecting pipe is connected with the sealed rotation of junction of sealed lid, and is a plurality of the equal fixedly connected with agitator of lower extreme lateral wall of connecting pipe, and is a plurality of the lateral wall of equal fixedly connected with a plurality of branches of connecting pipe, the ordinary hydraulic stem of inner wall fixedly connected with of culture tank body, ordinary hydraulic stem is located same level with branch.

Preferably, the peripheral side wall of the rotating groove is made of transparent glass, a power supply is arranged in the side wall of the lower end of the rotating groove, the plurality of light sources in the rotating groove are LED lamps, and the power supply is electrically connected with the light sources.

Preferably, the side wall of the culture tank body is provided with a sealing door, and the vertical height of the sealing door is greater than that of the culture cylinder.

Preferably, the upper end lateral wall of culture tank body is equipped with the filling tube, be equipped with control flap on the filling tube, the filling tube runs through the lateral wall of culture tank body, filling tube and the junction sealing connection of culture tank body lateral wall.

Preferably, the lateral wall of the upper end of the connecting pipe is fixedly connected with a funnel, and the diameter of the upper end of the funnel is larger than that of the feeding pipe.

Preferably, the movable end of the common hydraulic rod is fixedly connected with a limiting block, and the side wall of the limiting block is wrapped with a cushion pad.

Preferably, the inner wall of the culture tank body is fixedly connected with a temperature sensor and a heater, and the temperature sensor and the heater are positioned on two sides of the rotating rod.

Preferably, the lower extreme lateral wall fixedly connected with of blake jar body is a plurality of fixed feet, and is a plurality of the quantity of fixed foot is no less than four, and is a plurality of all the parcel has the rubber pad on the fixed foot.

Compared with the prior art, the invention has the beneficial effects that:

1. through the matching of the rotating motor, the light source, the first gear, the second gear and other structures, the culture cylinder can revolve around the light source and rotate, so that all plant cells in the culture cylinder receive uniform illumination, the problem of uneven development of the cultured plant cells caused by the problem of illumination angles is avoided, the culture effect of the plant cells is improved, and the culture rate of the plant cells is increased;

2. through the cooperation of structures such as agitator, connecting pipe, branch, ordinary hydraulic stem, can carry out slow stirring in adding the nutrient solution back to the culture cylinder, increase the contact opportunity of plant cell and nutrient solution, promote plant cell to the absorption of nutrition, alleviateed operating personnel's work burden simultaneously, increased work efficiency.

Drawings

FIG. 1 is a schematic front view of a plant cell culture tank according to the present invention;

FIG. 2 is a schematic top view of a first gear of a plant cell culture tank according to the present invention;

FIG. 3 is a schematic top view of a bearing of a plant cell culture tank according to the present invention;

fig. 4 is a schematic view of the structure at a in fig. 3.

In the figure: 1 sealing cover, 2 sealing door, 3 rotating groove, 4 power supply, 5 fixed block, 6 rotating motor, 7 funnel, 8 heater, 9 temperature sensor, 10 feed tube, 11 limit block, 12 common hydraulic rod, 13 connecting tube, 14 stirrer, 15 culture cylinder, 16 slide block, 17 motor groove, 18 fixed foot, 19 culture tank body, 20 annular rack, 21 first gear, 22 second gear, 23 light source, 24 bearing, 25 rotating rod, 26 support rod, 27 double-rod hydraulic rod, 28 slide rod, 29 slide groove, 30 slide block, 31 special-shaped block, 32 fixture block.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-4, a plant cell culture tank comprises a culture tank body 19 and a motor groove 17, wherein the inner wall of the motor groove 17 is fixedly connected with a rotating motor 6, an output shaft of the rotating motor 6 penetrates through the motor groove 17 and the side wall of the culture tank body 19, the output shaft of the rotating motor 6 is in sealed and rotating connection with the connection part of the motor groove 17 and the side wall of the culture tank body 19, the output shaft of the rotating motor 6 is fixedly connected with a first gear 21, the side wall of the culture tank body 19 is provided with an annular rack 20, the inner wall of the lower end of the culture tank body 19 is provided with an annular chute, a plurality of second gears 22 are arranged between the first gear 21 and the annular rack 20, the side wall of the lower end of the plurality of second gears 22 is fixedly connected with a slide block 16 matched with the annular chute, the side wall of the upper end of the plurality of second gears 22 is provided with a groove, the side wall of the upper end of the first gear 21 is provided with a rotating groove 3, a plurality of light sources 23 are arranged in the rotating groove 3, the peripheral side wall of the rotating groove 3 is provided with transparent glass, so as to be transparent, a power source 4 is arranged in the side wall of the rotating groove 3, the LED lamps, so that the LED lamps are arranged so that the heat sources 23 can not easily generate more heat, and the inner wall of the culture tank body 19 can be electrically connected with the temperature of the culture tank body, and the heat source 23;

the side wall of the upper end of the rotating groove 3 is fixedly connected with a rotating rod 25, one end of the rotating rod 25, which is far away from the rotating groove 3, is rotatably connected with the inner wall of the culture tank body 19, the side wall of the rotating rod 25 is fixedly connected with a plurality of sliding grooves 29, the inner walls of the plurality of sliding grooves 29 are all slidably connected with special-shaped blocks 31, the side walls of the two sides of the plurality of special-shaped blocks 31 are all provided with a plurality of clamping grooves, the inner walls of the two sides of the plurality of sliding grooves 29 are all provided with mounting grooves, two sliding rods 28 are fixedly connected between the inner walls of the two mounting grooves, the two sliding rods 28 are sleeved with the same sliding block 30, the side walls of the two sliding blocks 30 are all fixedly connected with a plurality of clamping blocks 32 matched with the clamping grooves, the plurality of clamping blocks 32 all penetrate through the side walls of the mounting grooves, the joints of the clamping blocks 32 and the side walls of the mounting grooves are in a sealing and sliding connection manner, the side walls of the sliding grooves 29 are all fixedly connected with two movable ends of the double-rod 27, the two movable ends of the double-rod 27 are respectively fixedly connected with the side walls of the two sliding blocks 30;

the side, far away from the sliding groove 29, of each of the special-shaped blocks 31 is fixedly connected with a bearing 24, the inner walls of the bearings 24 are fixedly connected with a culture cylinder 15, the lower end side walls of the culture cylinders 15 are fixedly connected with a fixed block 5 matched with the groove, the upper end side walls of the culture cylinders 15 are provided with sealing covers 1, the sealing covers 1 are fixedly connected with connecting pipes 13, the upper end side walls of the connecting pipes 13 are fixedly connected with a funnel 7, the upper end diameter of the funnel 7 is larger than that of a feeding pipe 10, nutrient solution dripping in the feeding pipe 10 is prevented from flowing out of the connecting pipes 13, all nutrient solution enters the culture cylinders 15, the connecting pipes 13 penetrate through the sealing covers 1, the joints of the connecting pipes 13 and the sealing covers 1 are in sealed and rotating connection, the lower end side walls of the connecting pipes 13 are fixedly connected with a stirrer 14, the side walls of the connecting pipes 13 are provided with a plurality of openings, the side walls of the connecting pipes 13 are fixedly connected with a plurality of supporting rods 26, the inner walls of the culture tank body 19 are fixedly connected with common hydraulic rods 12, the movable end side walls of the common hydraulic rods are fixedly connected with a limiting block 11, and the limiting block 11 is used for protecting the limiting block 11, and the service life of the limiting block 11 is prolonged;

cultivate jar body 19's lateral wall and be equipped with sealing door 2, sealing door 2's vertical height is greater than the vertical height of a culture section of thick bamboo 15, be convenient for get and put a culture section of thick bamboo 15, the upper end lateral wall of a culture section of thick bamboo 19 is equipped with filling tube 10, be equipped with control flap on the filling tube 10, filling tube 10 runs through the lateral wall of a culture section of thick bamboo 19, the junction sealing connection of filling tube 10 and a culture section of thick bamboo 19 lateral wall, be convenient for add nutrient solution in to a culture section of thick bamboo 15, control flap can keep the sealed of a culture section of thick bamboo body 19, the inner wall fixedly connected with temperature sensor 9 and heater 8 of a culture section of thick bamboo 19, be convenient for control the inside temperature of a culture section of thick bamboo 19, temperature sensor 9 and heater 8 are located the both sides of dwang 25, reduce the direct influence of heater 8 to temperature sensor 9, a plurality of fixed feet 18 of lower extreme lateral wall fixedly connected with of a culture section of thick bamboo body 19, the quantity of a plurality of fixed feet 18 is no less than four, all the parcel has the rubber pad on a plurality of fixed feet 18, avoid external vibration to the influence of internal culture section of a culture section of thick bamboo 15, also avoid external vibration to the damage of the whole device, increase the life of whole device.

When plant cells are cultured, a sealing cover 1 is opened, the plant cells and culture solution are added into a culture tank, then the sealing cover 1 is closed, a sealing door 2 is opened, a lower end fixing block 5 of a culture cylinder 15 body is aligned with a groove on a second gear 22 and a special-shaped block 31 on the side wall of a bearing 24 on the culture tank is aligned with a sliding groove 29 and is placed into a culture tank body 19, the movable end of a double-rod hydraulic rod 27 is withdrawn, the movable end of the double-rod hydraulic rod 27 drives a sliding block 30 to be special-shaped, the sliding block 30 drives a clamping block 32 to be clamped into a clamping groove on the side wall of the special-shaped block 31, so that the culture cylinder 15 is stably fixed, a rotating motor 6 runs, the rotating motor 6 drives a first gear 21 to rotate, the first gear 21 drives a rotating groove 3 and a light source 23 and a second gear 22 in the rotating groove to rotate, and the second gear 22 rotates under the action of an annular rack 20, so that all the plant cells in the culture cylinder 15 receive uniform illumination, the problem of uneven development of cultured plant cells caused by the problem of illumination angle is solved, the culture effect of the plant cells is improved, the culture rate of the plant cells is increased, when nutrient solution is added, the culture tank body 19 stops under the feeding pipe 10, the rotating motor 6 stops running temporarily, the nutrient solution drops from the feeding pipe 10 to the funnel 7 and then falls into the culture cylinder 15 from the opening of the side wall of the connecting pipe 13, the movable end of the common hydraulic rod 12 extends out, the rotating motor 6 continues running, when the culture tank is driven to be installed publicly, the connecting pipe 13 is driven to rotate by the common hydraulic rod 12, the supporting rod 26 of the side wall of the connecting pipe 13 collides with the limiting block 11, the connecting pipe 13 drives the stirrer 14 to rotate, slow stirring is carried out in the culture cylinder 15, the contact chance of the plant cells and the nutrient solution is increased, and the absorption of the plant cells to nutrition is promoted, meanwhile, the workload of operators is reduced, and the working efficiency is increased.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.

Claims

1. The utility model provides a plant cell culture tank, includes cultivates a jar body (19) and motor groove (17), its characterized in that, motor groove (17) inner wall fixed connection rotates motor (6), the output shaft that rotates motor (6) runs through motor groove (17) and cultivates the lateral wall of jar body (19), the output shaft and the motor groove (17) of rotating motor (6) and the sealed rotation of junction of cultivateing jar body (19) lateral wall are connected, the first gear of output shaft fixedly connected with (21) of rotating motor (6), the lateral wall of cultivateing jar body (19) is equipped with annular rack (20), the lower extreme inner wall of cultivateing jar body (19) is equipped with annular chute, this has a plurality of second gears (22) between first gear (21) and annular rack (20), and is a plurality of slider (16) that the lower extreme lateral wall fixedly connected with annular chute match of second gear (22), and a plurality of the upper end lateral wall of second gear (22) is equipped with the recess, the upper end lateral wall of first gear (21) is equipped with rotates groove (3), be equipped with a plurality of rotation groove (3), the one end of rotating groove (25) is connected with the culture tank body (3), the light source (25) of rotating groove (25), the side wall of the rotating rod (25) is fixedly connected with a plurality of sliding grooves (29), the inner walls of the plurality of sliding grooves (29) are all in sliding connection with special-shaped blocks (31), the side walls of the two sides of the plurality of special-shaped blocks (31) are all provided with a plurality of clamping grooves, the inner walls of the two sides of the plurality of sliding grooves (29) are all provided with mounting grooves, two sliding rods (28) are all fixedly connected between the inner walls of the two mounting grooves, the two sliding rods (28) are sleeved with the same sliding block (30), the side walls of the two sliding blocks (30) are all fixedly connected with a plurality of clamping blocks (32) matched with the clamping grooves, the plurality of clamping blocks (32) all penetrate through the side wall of the mounting groove, and the joints of the plurality of clamping blocks (32) and the side wall of the mounting groove are in sealing and sliding connection, equal fixedly connected with double-pole hydraulic stem (27) of lateral wall of sliding tray (29), the lateral wall that does not run through two mounting grooves is equallyd divide to two expansion ends of double-pole hydraulic stem (27), two expansion ends of double-pole hydraulic stem (27) are respectively with the sealed sliding connection of junction of two mounting groove lateral walls, two expansion ends of double-pole hydraulic stem (27) respectively with the lateral wall fixed connection of two sliding blocks (30), a plurality of equal fixedly connected with bearing (24) in one side of sliding tray (29) are kept away from in dysmorphism piece (31), and are a plurality of the equal fixedly connected with culture drum (15) of inner wall of bearing (24), it is a plurality of the equal fixedly connected with of lower extreme lateral wall of culture drum (15) is fixed block (5) that match with the recess It is a plurality of the upper end lateral wall of culture cylinder (15) is equipped with sealed lid (1), and is a plurality of sealed lid (1) equal fixedly connected with connecting pipe (13), and is a plurality of sealed lid (1) is all run through in connecting pipe (13), and is a plurality of connecting pipe (13) are rotated with the junction of sealed lid (1) is sealed, and is a plurality of equal fixedly connected with agitator (14) of lower extreme lateral wall of connecting pipe (13), and is a plurality of openings have been seted up to the lateral wall of connecting pipe (13), and is a plurality of equal fixedly connected with a plurality of branches (26) of lateral wall of connecting pipe (13), cultivate ordinary hydraulic stem (12) of inner wall fixedly connected with of jar body (19), ordinary hydraulic stem (12) are located same level with branch (26).

2. The plant cell culture tank of claim 1, wherein the peripheral side wall of the rotating tank (3) is made of transparent glass, a power supply (4) is arranged in the side wall of the lower end of the rotating tank (3), the plurality of light sources (23) in the rotating tank (3) are LED lamps, and the power supply (4) is electrically connected with the light sources (23).

3. Plant cell culture tank, according to claim 1, characterized in that the side wall of the tank body (19) is provided with a sealing door (2), the vertical height of the sealing door (2) being greater than the vertical height of the culture cylinder (15).

4. A plant cell culture tank according to claim 1, wherein the side wall of the upper end of the culture tank body (19) is provided with a feeding pipe (10), the feeding pipe (10) is provided with a control valve, the feeding pipe (10) penetrates through the side wall of the culture tank body (19), and the connection part of the feeding pipe (10) and the side wall of the culture tank body (19) is hermetically connected.

5. A plant cell culture tank according to claim 4, wherein a funnel (7) is fixedly connected to the upper side wall of the connecting pipe (13), and the diameter of the upper end of the funnel (7) is larger than that of the feeding pipe (10).

6. A plant cell culture tank according to claim 1, wherein the movable end of the common hydraulic rod (12) is fixedly connected with a limiting block (11), and the side wall of the limiting block (11) is wrapped with a buffer pad.

7. A plant cell culture tank according to claim 1, wherein a temperature sensor (9) and a heater (8) are fixedly connected to the inner wall of the culture tank body (19), and the temperature sensor (9) and the heater (8) are located on both sides of the rotation lever (25).

8. The plant cell culture tank as claimed in claim 1, wherein a plurality of fixing feet (18) are fixedly connected to the side wall of the lower end of the culture tank body (19), the number of the fixing feet (18) is not less than four, and rubber pads are wrapped on the plurality of fixing feet (18).