CN212247077U

CN212247077U - Swing type cell culture device

Info

Publication number: CN212247077U
Application number: CN202020374007.0U
Authority: CN
Inventors: 闫玉波; 张克瑞
Original assignee: Shenzhen Cell Inspire Biotechnology Co ltd
Current assignee: Shenzhen Cell Inspire Biotechnology Co ltd
Priority date: 2020-03-23
Filing date: 2020-03-23
Publication date: 2020-12-29
Anticipated expiration: 2030-03-23

Abstract

The utility model provides a swing type cell culture device, which comprises a shell, a support plate, a slide seat, a culture bottle, a swing mechanism and a rotating mechanism; the shell is provided with a switch door, and the supporting plate is horizontally fixed in the shell; the support plate is provided with a long and narrow sliding slit, the sliding seat is positioned on the upper surface of the support plate, and the sliding seat is arranged in the slit in a sliding manner; the culture bottle is rotatably arranged on the upper surface of the sliding seat, and the upper surface of the sliding seat is also provided with a rotating mechanism for driving the culture bottle to rotate; and a swing mechanism is arranged below the supporting plate, the swing mechanism is connected with the part of the sliding seat penetrating through the sliding seam, and the swing mechanism is used for driving the sliding seat to move. Swing formula cell culture device can avoid agitating unit to the shearing of cell destroy and bring into the condition of miscellaneous fungus.

Description

Swing type cell culture device

Technical Field

The utility model belongs to the technical field of the cell culture technique and specifically relates to a formula cell culture device sways is related to.

Background

At present, the cell culture mode mainly adopts the culture of suspension cells, the suspension cells are statically placed in the culture process, and the proliferation of the cells is limited because the culture solution is static and the nutrient solution is unevenly consumed in the cell growth process. Therefore, in order to obtain higher cell densities, it is necessary to make the cell culture dynamic.

At present, in order to realize dynamic cell culture, a stirring device is generally inserted into a reactor to promote the flow of liquid, and the stirring mode causes shearing force formed by liquid flow or a rotor to easily damage cells; moreover, the stirring device structure and the lubricating substance thereof often bring about the problem of microbial contamination, the occurrence rate of the condition that the stirring and lubricating bring in the mixed bacteria is high, and once the mixed bacteria are contaminated, the cell death can be caused.

SUMMERY OF THE UTILITY MODEL

In view of this, the present invention provides a swing cell culture device, which can avoid shearing damage to cells and bringing in bacteria by a stirring device.

In order to achieve the above purpose, the technical scheme of the utility model is realized like this:

a swing type cell culture device comprises a shell, a support plate, a slide seat, a culture bottle, a swing mechanism and a rotating mechanism;

the shell is provided with a switch door, and the supporting plate is horizontally fixed in the shell; the support plate is provided with a long and narrow sliding slit, the sliding seat is positioned on the upper surface of the support plate, and the sliding seat is arranged in the slit in a sliding manner; the culture bottle is rotatably arranged on the upper surface of the sliding seat, and the upper surface of the sliding seat is also provided with a rotating mechanism for driving the culture bottle to rotate; and a swing mechanism is arranged below the supporting plate, the swing mechanism is connected with the part of the sliding seat penetrating through the sliding seam, and the swing mechanism is used for driving the sliding seat to move.

Further, the sliding seat comprises a supporting table and a sliding block fixed on the lower surface of the supporting table, and the supporting table and the sliding block form a T-shaped structure; the supporting table is located on the upper surface of the supporting plate, the sliding block penetrates through the sliding seam and can move in the sliding seam, and the swinging mechanism is connected with the sliding block.

Further, the swing mechanism comprises a connecting rod, a swing arm, a driving wheel, a transmission rod, a bracket, a third motor and a supporting rod;

the upper end of the connecting rod is hinged to the sliding block, and the lower end of the connecting rod is hinged to the upper end of the swing arm; a support rod is fixed at the lower part of the side wall of the shell, and the lower end of the swing arm is rotatably arranged on the support rod; one side of the swing arm is provided with a bracket, the bracket is rotatably provided with a driving wheel, and the central axis of the driving wheel is parallel to the central axis of a hinged shaft of the connecting rod and the swing arm; a cylindrical transmission rod is fixedly arranged on one side, opposite to the swing arm, of the transmission wheel, and a central shaft of the transmission wheel and a central shaft of the transmission rod are parallel but not collinear; the swing arm is provided with a long and narrow through hole, the transmission rod penetrates through the through hole, and the transmission rod moves up and down in the through hole along with the rotation of the transmission wheel; the transmission wheel is driven by a third motor.

Further, the lower surface of slider is equipped with the recess, the upper end of connecting rod stretches into in the recess and is articulated with the lateral wall of recess.

Further, the rotating mechanism comprises a driven gear, a first incomplete gear, a first motor, a second incomplete gear and a second motor;

the driven gear is fixedly sleeved on the side wall of the lower part of the culture bottle, a first incomplete gear and a second incomplete gear are rotationally arranged on the upper surface of the support table, and the first incomplete gear and the second incomplete gear are both meshed with the driven gear; first incomplete gear and second incomplete gear are by first motor and second motor drive respectively, and first incomplete gear and second incomplete gear rotation direction are opposite to realize that the blake bottle can realize forward and reverse rotation in turn under first incomplete gear and the drive of second incomplete gear.

Further, the bottom of the culture bottle is arc-shaped, and the upper surface of the supporting table is provided with an arc-shaped placing groove matched with the bottom of the culture bottle.

Furthermore, a plurality of ball grooves are formed in the side wall of the placing groove, balls are arranged in the ball grooves in a rolling mode, and the balls are in contact with the bottom of the culture bottle.

Furthermore, a sliding groove is formed in the side wall of the sliding seam, and a clamping piece matched with the sliding groove for use is arranged on the side wall, inserted into the sliding seam, of the sliding block.

Furthermore, an observation window is arranged on the opening and closing door.

Compared with the prior art, the swing type cell culture device has the following advantages:

(1) the swing cell culture device of the utility model comprises a shell, a support plate, a slide seat, a culture bottle, a swing mechanism and a rotating mechanism; the backup pad level is fixed in the inside of shell, and the slide slides and sets up in the smooth seam in the backup pad, and the blake bottle rotates the upper surface that sets up at the slide to by the slewing mechanism drive of setting at the upper surface of slide and rotate, the below of backup pad is equipped with and is connected with the slide and drive the swing mechanism that the slide removed. In the using process, the swing mechanism drives the sliding seat to move back and forth on the supporting plate, so as to drive the culture bottle to move back and forth; the rotating mechanism drives the culture bottle to rotate, so that the culture solution in the culture bottle is in a dynamic state under the action of the swinging mechanism and the rotating mechanism, and the condition that the proliferation of cells is limited due to unbalanced consumption of the culture solution is avoided; meanwhile, the conditions that the stirring device shears and damages cells and brings in mixed bacteria are avoided.

(2) The swing mechanism of the utility model comprises a connecting rod, a swing arm, a driving wheel, a transmission rod, a bracket, a third motor and a support rod; the connecting rod is hinged with the swing arm, the lower end of the swing arm is rotatably arranged on the supporting rod, a driving wheel is rotatably arranged on the support arranged on one side of the swing arm, and a driving rod inserted into the through hole in the swing arm is arranged on the driving wheel. When the driving wheel rotates under the driving of the third motor, the transmission rod moves up and down in the through hole along with the rotation of the driving wheel, and then the swing arm is driven to swing left and right, and the sliding seat reciprocates on the supporting plate.

(3) The rotating mechanism of the utility model comprises a driven gear, a first incomplete gear, a first motor, a second incomplete gear and a second motor; the driven gear is fixedly sleeved on the side wall of the lower portion of the culture bottle, the first incomplete gear and the second incomplete gear are rotatably arranged on the upper surface of the supporting table, the first incomplete gear and the second incomplete gear are meshed with the driven gear, and the rotating directions of the first incomplete gear and the second incomplete gear are opposite. The setting of first incomplete gear and second incomplete gear makes the blake bottle realize forward and reverse rotation in turn under their drive to make the culture solution in the blake bottle be in dynamic state.

(4) Supporting bench's upper surface is equipped with the curved mounting groove that coincide mutually with the bottom of blake bottle, is equipped with a plurality of ball grooves on the lateral wall of mounting groove, and the ball inslot rolls and is equipped with the ball, the bottom contact of ball and blake bottle, and the setting up of ball has reduced the friction between blake bottle and the mounting groove, is favorable to the rotation of blake bottle.

Drawings

The accompanying drawings, which form a part hereof, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without undue limitation. In the drawings:

FIG. 1 is a schematic structural view of a rocking cell culture apparatus according to an embodiment of the present invention;

FIG. 2 is a perspective view of a rocking cell culture device according to an embodiment of the present invention;

FIG. 3 is a view showing an internal structure of a rocking cell culture apparatus according to an embodiment of the present invention;

fig. 4 is a schematic structural diagram of a swing mechanism according to an embodiment of the present invention;

fig. 5 is a top view of a rotating mechanism according to an embodiment of the present invention;

fig. 6 is a schematic structural diagram of a supporting table according to an embodiment of the present invention;

fig. 7 is a cross-sectional view of a support table according to an embodiment of the present invention.

Description of reference numerals:

1-a housing; 2-a support plate; 3-sliding the seam; 4-a chute; 5-a slide seat; 6-clamping piece; 7-a groove; 8-a connecting rod; 9-swing arm; 10-a through hole; 11-a transmission wheel; 12-a transmission rod; 13-a scaffold; 14-culture flask; 15-a driven gear; 16-a first incomplete gear; 17-a first electric machine; 18-a second partial gear; 19-a second electric machine; 20-supporting table; 21-a slide block; 22-a viewing window; 23-a support bar; 24-a third motor; 25-placing grooves; 26-a ball groove; 27-ball.

Detailed Description

It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

Example 1

FIG. 1 is a schematic structural view of a rocking cell culture apparatus according to an embodiment of the present invention.

A swing type cell culture device comprises a shell 1, a support plate 2, a slide seat 5, a culture bottle 14, a swing mechanism and a rotating mechanism;

the shell 1 is provided with a switch door, and the support plate 2 is horizontally fixed in the shell 1; a long and narrow sliding slit 3 is arranged on the supporting plate 2, the sliding seat 5 is positioned on the upper surface of the supporting plate 2, and the sliding seat 5 is arranged in the slit 3 in a sliding manner; the culture bottle 14 is rotatably arranged on the upper surface of the sliding seat 5, and the upper surface of the sliding seat 5 is also provided with a rotating mechanism for driving the culture bottle 14 to rotate; and a swing mechanism is arranged below the supporting plate 2, the swing mechanism is connected with the part of the sliding seat 5 penetrating through the sliding seam 3, and the swing mechanism is used for driving the sliding seat 5 to move.

The working principle of the swing type cell culture device in the embodiment is as follows:

the swing type cell culture device comprises a shell 1, a supporting plate 2, a sliding seat 5, a culture bottle 14, a swing mechanism and a rotating mechanism; the support plate 2 is horizontally fixed in the shell 1, the sliding seat 5 is arranged in the sliding seam 3 on the support plate 2 in a sliding mode, the culture bottle 14 is arranged on the upper surface of the sliding seat 5 in a rotating mode and driven to rotate by the rotating mechanism arranged on the upper surface of the sliding seat 5, and the swing mechanism which is connected with the sliding seat 5 and drives the sliding seat 5 to move is arranged below the support plate 2.

In the using process, the swing mechanism drives the sliding seat 5 to move back and forth on the supporting plate 2, and further drives the culture bottle 14 to move back and forth; the rotating mechanism drives the culture bottle 14 to rotate, so that the culture solution in the culture bottle is in a dynamic state under the action of the swinging mechanism and the rotating mechanism, and the condition that the proliferation of cells is limited due to unbalanced consumption of the culture solution is avoided; meanwhile, the conditions that the stirring device shears and damages cells and brings in mixed bacteria are avoided.

Example 2

Fig. 4 is a schematic structural diagram of a swing mechanism according to an embodiment of the present invention.

On the basis of the above embodiment, the slide carriage 5 exemplarily includes a support table 20 and a slide block 21 fixed on the lower surface of the support table 20, and the support table 20 and the slide block 21 form a T-shaped structure; the supporting table 20 is positioned on the upper surface of the supporting plate 2, the sliding block 21 penetrates through the sliding slot 3 and can move in the sliding slot 3, and the swinging mechanism is connected with the sliding block 21.

Specifically, as shown in fig. 4, the swing mechanism includes a connecting rod 8, a swing arm 9, a driving wheel 11, a transmission rod 12, a bracket 13, a third motor 24 and a support rod 23;

the upper end of the connecting rod 8 is hinged on the sliding block 21, and the lower end of the connecting rod 8 is hinged with the upper end of the swing arm 9; a support rod 23 is fixed at the lower part of the side wall of the shell 1, and the lower end of the swing arm 9 is rotatably arranged on the support rod 23; one side of the swing arm 9 is provided with a bracket 13, the bracket 13 is rotatably provided with a driving wheel 11, and the central axis of the driving wheel 11 is parallel to the central axis of a hinged shaft of the connecting rod 8 and the swing arm 9; a cylindrical transmission rod 12 is fixedly arranged on one side, opposite to the swing arm 9, of the transmission wheel 11, and a central shaft of the transmission wheel 11 and a central shaft of the transmission rod 12 are parallel but not collinear; a long and narrow through hole 10 is formed in the swing arm 9, the transmission rod 12 penetrates through the through hole 10, and the transmission rod 12 moves up and down in the through hole 10 along with the rotation of the transmission wheel 11; the drive wheel 11 is driven by a third motor 24.

More specifically, the lower surface of the sliding block 21 is provided with a groove 7, and the upper end of the connecting rod 8 extends into the groove 7 and is hinged with the side wall of the groove 7.

the swing mechanism comprises a connecting rod 8, a swing arm 9, a driving wheel 11, a transmission rod 12, a bracket 13, a third motor 24 and a supporting rod 23; the connecting rod 8 is hinged with the swing arm 9, the lower end of the swing arm 9 is rotatably arranged on the supporting rod 23, a driving wheel 11 is rotatably arranged on a support 13 arranged on one side of the swing arm 9, and a driving rod 12 inserted into a through hole 10 on the swing arm 9 is arranged on the driving wheel 11. When the driving wheel 11 is driven by the third motor 24 to rotate, the transmission rod 12 moves up and down in the through hole 10 along with the rotation of the driving wheel 11, and further drives the swing arm 9 to swing left and right, so that the sliding seat 5 reciprocates on the supporting plate 2.

Example 3

FIG. 2 is a perspective view of a rocking cell culture device according to an embodiment of the present invention; FIG. 3 is a view showing an internal structure of a rocking cell culture apparatus according to an embodiment of the present invention; fig. 5 is a top view of a rotating mechanism according to an embodiment of the present invention; fig. 6 is a schematic structural diagram of a supporting table according to an embodiment of the present invention; fig. 7 is a cross-sectional view of a support table according to an embodiment of the present invention.

On the basis of the above embodiment, exemplarily, as shown in fig. 3 and 5, the rotating mechanism includes the driven gear 15, the first incomplete gear 16, the first motor 17, the second incomplete gear 18, and the second motor 19;

the driven gear 15 is fixedly sleeved on the side wall of the lower part of the culture bottle 14, the upper surface of the support platform 20 is rotatably provided with a first incomplete gear 16 and a second incomplete gear 18, and the first incomplete gear 16 and the second incomplete gear 18 are both meshed with the driven gear 15; the first incomplete gear 16 and the second incomplete gear 18 are respectively driven by a first motor 17 and a second motor 19, and the rotation directions of the first incomplete gear 16 and the second incomplete gear 18 are opposite, so that the culture bottles 14 can realize forward and reverse alternate rotation under the drive of the first incomplete gear 16 and the second incomplete gear 18.

Specifically, the bottom of the culture bottle 14 is arc-shaped, and the upper surface of the support platform 20 is provided with an arc-shaped mounting groove 25 which is matched with the bottom of the culture bottle 14.

More specifically, as shown in fig. 6 and 7, the side wall of the receiving groove 25 is provided with a plurality of ball grooves 26, and balls 27 are arranged in the ball grooves 26 in a rolling manner, and the balls 27 are in contact with the bottom of the culture bottle 14.

Illustratively, a sliding groove 4 is arranged on the side wall of the sliding gap 3, and a clamping piece 6 matched with the sliding groove 4 is arranged on the side wall of the sliding gap 3 where the sliding block 21 is inserted.

Illustratively, as shown in fig. 2, the opening and closing door is provided with a viewing window 22.

the rotating mechanism comprises a driven gear 15, a first incomplete gear 16, a first motor 17, a second incomplete gear 18 and a second motor 19; the driven gear 15 is fixedly sleeved on the side wall of the lower part of the culture bottle 14, the first incomplete gear 16 and the second incomplete gear 18 are rotatably arranged on the upper surface of the support platform 20, the first incomplete gear 16 and the second incomplete gear 18 are both meshed with the driven gear 15, and the rotating directions of the first incomplete gear 16 and the second incomplete gear 18 are opposite. The first incomplete gear 16 and the second incomplete gear 18 are arranged to enable the culture bottle 14 to rotate forward and backward alternately under the driving of the first incomplete gear and the second incomplete gear, so that the culture solution in the culture bottle is in a dynamic state.

The upper surface of the supporting platform 20 is provided with an arc-shaped mounting groove 25 which is matched with the bottom of the culture bottle 14, the side wall of the mounting groove 25 is provided with a plurality of ball grooves 26, balls 27 are arranged in the ball grooves 26 in a rolling manner, the balls 27 are in contact with the bottom of the culture bottle 14, and the arrangement of the balls 27 reduces the friction between the culture bottle 14 and the mounting groove 25, so that the rotation of the culture bottle is facilitated.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A rocking cell culture device, characterized in that: comprises a shell (1), a supporting plate (2), a sliding seat (5), a culture bottle (14), a swinging mechanism and a rotating mechanism;

the switch door is arranged on the shell (1), and the support plate (2) is horizontally fixed in the shell (1); the supporting plate (2) is provided with a long and narrow sliding seam (3), the sliding seat (5) is positioned on the upper surface of the supporting plate (2), and the sliding seat (5) is arranged in the sliding seam (3) in a sliding manner; the culture bottle (14) is rotatably arranged on the upper surface of the sliding seat (5), and the upper surface of the sliding seat (5) is also provided with a rotating mechanism for driving the culture bottle (14) to rotate; the lower part of the supporting plate (2) is provided with a swing mechanism, the swing mechanism is connected with the part of the sliding seat (5) penetrating through the sliding seam (3), and the swing mechanism is used for driving the sliding seat (5) to move.

2. The rocking cell culture device of claim 1, wherein: the sliding seat (5) comprises a supporting platform (20) and a sliding block (21) fixed on the lower surface of the supporting platform (20), and the supporting platform (20) and the sliding block (21) form a T-shaped structure; the supporting table (20) is located on the upper surface of the supporting plate (2), the sliding block (21) penetrates through the sliding gap (3) and can move in the sliding gap (3), and the swinging mechanism is connected with the sliding block (21).

3. The rocking cell culture device of claim 2, wherein: the swing mechanism comprises a connecting rod (8), a swing arm (9), a transmission wheel (11), a transmission rod (12), a bracket (13), a third motor (24) and a support rod (23);

the upper end of the connecting rod (8) is hinged to the sliding block (21), and the lower end of the connecting rod (8) is hinged to the upper end of the swing arm (9); a support rod (23) is fixed at the lower part of the side wall of the shell (1), and the lower end of the swing arm (9) is rotatably arranged on the support rod (23); a bracket (13) is arranged on one side of the swing arm (9), a driving wheel (11) is rotatably arranged on the bracket (13), and the central axis of the driving wheel (11) is parallel to the central axis of a hinged shaft of the connecting rod (8) and the swing arm (9); a cylindrical transmission rod (12) is fixedly arranged on one side, opposite to the swing arm (9), of the transmission wheel (11), and the central axis of the transmission wheel (11) is parallel to the central axis of the transmission rod (12) but not collinear; a long and narrow through hole (10) is formed in the swing arm (9), the transmission rod (12) penetrates through the through hole (10), and the transmission rod (12) moves up and down in the through hole (10) along with the rotation of the transmission wheel (11); the transmission wheel (11) is driven by a third motor (24).

4. A rocking cell culture device according to claim 3, wherein: the lower surface of the sliding block (21) is provided with a groove (7), and the upper end of the connecting rod (8) extends into the groove (7) and is hinged with the side wall of the groove (7).

5. The rocking cell culture device of claim 2, wherein: the rotating mechanism comprises a driven gear (15), a first incomplete gear (16), a first motor (17), a second incomplete gear (18) and a second motor (19);

the driven gear (15) is fixedly sleeved on the side wall of the lower part of the culture bottle (14), the upper surface of the support platform (20) is rotatably provided with a first incomplete gear (16) and a second incomplete gear (18), and the first incomplete gear (16) and the second incomplete gear (18) are both meshed with the driven gear (15); the first incomplete gear (16) and the second incomplete gear (18) are respectively driven by a first motor (17) and a second motor (19), and the rotating directions of the first incomplete gear (16) and the second incomplete gear (18) are opposite, so that the culture bottles (14) can be driven by the first incomplete gear (16) and the second incomplete gear (18) to rotate forward and reversely alternately.

6. A rocking cell culture device according to claim 5, wherein: the bottom of the culture bottle (14) is arc-shaped, and the upper surface of the support platform (20) is provided with an arc-shaped placing groove (25) which is matched with the bottom of the culture bottle (14).

7. The rocking cell culture device of claim 6, wherein: the side wall of the placing groove (25) is provided with a plurality of ball grooves (26), balls (27) are arranged in the ball grooves (26) in a rolling mode, and the balls (27) are in contact with the bottom of the culture bottle (14).

8. The rocking cell culture device of claim 2, wherein: the side wall of the sliding seam (3) is provided with a sliding groove (4), and the sliding block (21) is inserted into the side wall inside the sliding seam (3) and is provided with a clamping piece (6) matched with the sliding groove (4).

9. The rocking cell culture device of claim 1, wherein: an observation window (22) is arranged on the switch door.