CN215654951U

CN215654951U - Raw material dissolving device for serum-free cell culture medium preparation

Info

Publication number: CN215654951U
Application number: CN202122380985.3U
Authority: CN
Inventors: 罗丽; 赵琼英; 金顺兰; 白雅珊; 黎艳; 杨庆芳; 王国东; 李平芬; 张晏榕
Original assignee: Yunnan Rick Biotechnology Co ltd
Current assignee: Yunnan Rick Biotechnology Co ltd
Priority date: 2021-09-29
Filing date: 2021-09-29
Publication date: 2022-01-28
Anticipated expiration: 2031-09-29

Abstract

The application discloses serum-free cell culture medium is prepared with raw materials dissolving device includes: an eccentric shaft drive assembly; the device comprises a vibration barrel, a liquid transferring barrel, a guide mounting plate, a bracket and a hose pump; the middle part of the guide mounting plate is provided with a mounting hole, and a plurality of sliding rollers are mounted on the inner side wall of the guide mounting plate; the vibrating barrel is inserted into a mounting hole formed in the guide mounting plate, and the side wall of the vibrating barrel is in rolling contact with the sliding roller; the bottom surface of the guide mounting plate is provided with a bracket; the eccentric shaft driving assembly is arranged below the bracket and is in driving connection with the bottom surface of the vibration barrel; the eccentric shaft driving component drives the vibration barrel to move up and down along the central axis of the guide mounting plate; this application is through adopting the hose pump to holding pulse pump sending solvent in the vibration bucket of placing the cell culture medium and treating dissolving the powder raw materials, can utilize pulse liquid column, breaks up the reunion powder raw materials that the liquid feeding initial stage formed, improves raw materials initial stage dissolution efficiency, reduces the proportion that raise dust and aggregate produced.

Description

Raw material dissolving device for serum-free cell culture medium preparation

Technical Field

The application relates to the technical field of cell culture, in particular to a raw material dissolving device for preparing a serum-free cell culture medium.

Background

In order to research various performances of cells, the cells are required to be subjected to in vitro amplification culture, and the serum-free culture medium refers to a synthetic culture medium which can maintain the cells to grow and propagate in vitro for a long time without adding serum. The basic formula of the serum-free culture medium comprises two major components, namely a basic culture medium and an additive component. The basic culture medium used by the existing serum-free culture medium is prepared by dissolving a powder raw material in ultrapure water.

Although the existing powder raw material can be dissolved in water, in mass production, because the particle size of the powder material is small, dust is easy to raise in the initial dissolving stage, aggregates are formed and are difficult to dissolve in water, and the existing powder raw material mostly adopts a magnetic stirrer to carry out vibration stirring, so that the vibration force is small, and the added ultrapure water cannot be effectively utilized to accelerate the initial dissolving speed of the raw material.

SUMMERY OF THE UTILITY MODEL

The present application provides a raw material dissolving device for serum-free cell culture medium preparation for solving the above-mentioned technical problems.

The application provides a serum-free cell culture medium prepares uses raw materials dissolving device, includes: an eccentric shaft drive assembly; the device comprises a vibration barrel, a liquid transferring barrel, a guide mounting plate, a bracket and a hose pump; the middle part of the guide mounting plate is provided with a mounting hole, and a plurality of sliding rollers are mounted on the inner side wall of the guide mounting plate;

the vibrating barrel is inserted into a mounting hole formed in the guide mounting plate, and the side wall of the vibrating barrel is in rolling contact with the sliding roller; the bottom surface of the guide mounting plate is provided with a bracket; the eccentric shaft driving assembly is arranged below the bracket and is in driving connection with the bottom surface of the vibration barrel; the eccentric shaft driving component drives the vibration barrel to move up and down along the central axis of the guide mounting plate;

a liquid transferring barrel is arranged in the vibration barrel; the top surface of the liquid transferring barrel is provided with a threaded cover; the top surface of the threaded cover is provided with a through hole; a flange cover is arranged on the upper cover of the top surface of the vibration barrel; the flange cover is provided with a liquid inlet pipe; the liquid inlet pipe is inserted in the through hole and is communicated with the liquid outlet end pipeline of the hose pump.

Preferably, the eccentric shaft driving assembly includes: the driving motor, the coupling, the bearing seat, the rotating shaft, the eccentric shaft and the hinge piece; the driving motor is in driving connection with the rotating shaft through a coupler; the rotating shaft is rotatably arranged in the bearing seat; the eccentric shaft is arranged on the far end face of the rotating shaft and is connected with the vibrating barrel through a hinge piece.

Preferably, the method comprises the following steps: a connecting rod hinge; the link hinge includes: a connecting rod, a hinge shaft and a driving rod; one end of the connecting rod is sleeved on the eccentric shaft; the other end of the connecting rod is hinged with the driving rod through a hinge shaft.

Preferably, the method comprises the following steps: the guide support plate, the guide shaft seat, the inclined plate and the cylinder seat; the guide support plate is arranged on the end face of the bearing seat, a hole is formed in the guide support plate, and the guide shaft seat is arranged in the hole; the driving rod is inserted in the guide shaft seat; the other end of the driving rod is connected with the bottom surface of the cylinder seat; a plurality of inclined plates are arranged on the outer side wall of the other end of the driving rod at intervals.

Preferably, the method comprises the following steps: a sealing cover; the sealing cover is arranged on the first end surface of the liquid inlet pipe in a covering mode.

Preferably, the pipetting barrel comprises: the flange edge is clamped between the vibration barrel and the flange cover; the threaded cover is inserted in the barrel body and is in threaded connection with the inner side wall of the upper part of the barrel body; the top surface of the threaded cover is provided with a handle, so that the liquid moving barrel can be conveniently opened through the handle.

The beneficial effects that this application can produce include:

1) the application provides a serum-free cell culture medium prepares uses raw materials dissolving device, this application can utilize the pulse liquid column through adopting the hose pump to holding the pulse pump sending solvent in placing the vibration bucket that the cell culture medium waited to dissolve the powder raw materials, breaks up the reunion powder raw materials that the liquid feeding initial stage formed, improves raw materials initial stage dissolution efficiency, reduces the proportion that raise dust and aggregate produced.

2) The application provides a serum-free cell culture medium prepares uses raw materials dissolving device, through at vibration bucket bottom installation vibration base, after accomplishing the liquid feeding, promotes wherein the raw materials to dissolve through eccentric shaft drive assembly drive vibration bucket vibration, and the vibrational force of vibration bucket is greater than magnetic stirrers, can effectively shorten the vibration and promote the time of dissolving, improves cell culture medium preparation efficiency.

Drawings

FIG. 1 is a schematic front view of a raw material dissolving device for preparing serum-free cell culture medium provided by the present application;

fig. 2 is a schematic front view of a vibration barrel provided in the present application;

fig. 3 is a schematic front view of a pipetting barrel provided by the present application;

FIG. 4 is a schematic top view of the vibration barrel provided in the present application;

FIG. 5 is a schematic front view of a hose pump used in the present application;

FIG. 6 is a side view of the eccentric shaft drive assembly connection provided by the present application;

illustration of the drawings:

10. a drive motor; 11. a coupling; 112. a bearing seat; 113. a rotating shaft; 117. an eccentric shaft; 115. a connecting rod; 126. hinging a shaft; 114. a drive rod; 116. a guide support plate; 124. a guide shaft seat; 125. a sloping plate; 127. a cartridge holder; 21. vibrating the barrel; 23. a flange cover; 211. a sealing cover; 212. a liquid inlet pipe; 311. a liquid inlet; 313. a hose pump; 314. a pump motor; 312. a liquid outlet; 315. an observation window; 22. a liquid transferring barrel; 221. a threaded cap; 224. a through hole; 12. a guide mounting plate; 121. mounting grooves; 133. a sliding roller; 123. and (4) a roll shaft.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

In addition, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships that are conventionally placed when the products of the present invention are used, or orientations or positional relationships that are conventionally understood by those skilled in the art, and are used only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element that is referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Technical means which are not described in detail in the present application and are not used for solving the technical problems of the present application are all arranged according to common knowledge in the field, and various common knowledge arrangement modes can be realized.

Referring to fig. 1 to 6, the present application provides a raw material dissolving device for preparing a serum-free cell culture medium, including: the eccentric shaft 117 driving component, the vibration barrel 21, the liquid-transferring barrel 22, the guide mounting plate 12, the bracket and the hose pump 313; the middle part of the guide mounting plate 12 is provided with a mounting hole, and a plurality of sliding rollers 133 are mounted on the inner side wall of the guide mounting plate;

the vibration barrel 21 is inserted into the mounting hole formed in the guide mounting plate 12, and the side wall of the vibration barrel 21 is in rolling contact with the sliding roller 133; a bracket is arranged on the bottom surface of the guide mounting plate 12; the eccentric shaft 117 driving component is arranged below the bracket and is in driving connection with the bottom surface of the vibration barrel 21; the eccentric shaft 117 driving component drives the vibration barrel 21 to move up and down along the central axis of the guide mounting plate 12;

a liquid transferring barrel 22 is arranged in the vibrating barrel 21; a threaded cover 221 is arranged on the top surface of the liquid transferring barrel 22; the top surface of the screw cap 221 is provided with a through hole 224; the upper cover of the top surface of the vibration barrel 21 is provided with a flange cover 23; the flange cover 23 is provided with a liquid inlet pipe 212; the liquid inlet pipe 212 is inserted into the through hole 224 and is communicated with a liquid outlet pipeline of the hose pump 313.

When powder material is dissolved according to the setting, accessible hose pump 313 is to vibration bucket 21 interior pulse pump sending cell culture medium dissolve with solvent, effectively utilizes the water column to break up powder aggregate, improves powder dissolving efficiency, and through eccentric shaft 117 drive assembly, drive vibration bucket 21 is along its vertical reciprocating motion, replaces magnetic stirrers or manual rocking, can improve powder material dissolving efficiency simultaneously.

When in use, the powder material is added into the liquid transferring barrel 22, and the liquid transferring barrel 22 is arranged in the vibrating barrel 21; when the top surfaces of the vibration bucket 21 and the pipette bucket 22 are closed, the liquid inlet tube 212 is inserted into the through hole 224 of the screw cap 221 and is connected to the hose pump 313. The hose pump 313 is started to feed liquid, and the eccentric driving assembly is started after the liquid feeding is finished, so that the powder raw materials are effectively dissolved.

The eccentric shaft 117 drive assembly, and hose pump 313, used in this application, may be arranged in a manner common in the art and will not be described in detail herein. The liquid outlet amount of the hose pump 313 can be selected according to the requirement.

The used solvent can be ultrapure water, the microorganism content can be reduced, the purity is improved, the device can be operated in a biological safety cabinet according to the requirement of dissolving raw materials, and the sterilization treatment of the cell culture medium can also be realized through filtration sterilization after the culture medium is dissolved and prepared.

Preferably, the eccentric shaft 117 drive assembly comprises: the driving motor 10, the coupling 11, the bearing seat 112, the rotating shaft 113, the eccentric shaft 117 and the hinge piece; the driving motor 10 is in driving connection with the rotating shaft 113 through the coupler 11; the rotating shaft 113 is rotatably installed in the bearing seat 112; the eccentric shaft 117 is mounted on the distal end surface of the rotation shaft 113 and is connected to the vibration tub 21 by a hinge.

Preferably, the method comprises the following steps: a connecting rod hinge; the connecting rod hinge member includes: link 115, hinge shaft 126, drive rod 114; one end of the connecting rod 115 is sleeved on the eccentric shaft 117; the other end of link 115 is hinged to drive rod 114 via hinge shaft 126.

Preferably, the method comprises the following steps: a guide support plate 116, a guide shaft seat 124, a sloping plate 125 and a cylinder seat 127; the guide support plate 116 is arranged on the end surface of the bearing seat 112, a hole is arranged on the guide support plate 116, and the guide shaft seat 124 is arranged in the hole; the driving rod 114 is inserted into the guiding shaft seat 124; the other end of the driving rod 114 is connected with the bottom surface of the cylinder seat 127; a plurality of inclined plates 125 are arranged on the outer side wall of the other end of the driving rod 114 at intervals. Thereby improving the vibration smoothness of the vibration tub 21.

Preferably, the method comprises the following steps: a sealing cover 211; the sealing cover 211 is covered on the first end surface of the liquid inlet pipe 212. Through the arrangement of the sealing cover 211, impurity pollution can be reduced during vibration.

In one embodiment, the hose pump 313 includes: pump motor 314, housing, viewing window 315; the pump motor 314 is in driving connection with a rotating part inside the shell; an observation window 315 is arranged on the end face of the shell; the shell is internally provided with a pipe fitting and a rotating part, and a liquid outlet 312 and a liquid inlet 311 of the pipe fitting extend out of the shell.

Preferably, the pipette barrel 22 comprises: the vibration barrel comprises a barrel body and a flange edge, wherein the flange edge is clamped between the vibration barrel 21 and the flange cover 23; the threaded cover 221 is inserted into the barrel body and is in threaded connection with the inner side wall of the upper part of the barrel body; the top surface of the threaded cap 221 is provided with a handle, which is convenient for opening the liquid transferring barrel 22. Vibration bucket 21 and flange accessible bolt assembly are connected, can realize the effective centre gripping to move liquid bucket 22 when the vibration according to this setting, avoid its removal.

In one embodiment, the method comprises the following steps: a plurality of mounting grooves 121, a roller shaft 123; the mounting groove 121 is arranged on the inner side wall of the central hole of the guide mounting plate 12; the roller is inserted on the sliding roller 133 and installed in the installation groove 121, and the outer sidewall of the sliding roller 133 protrudes out of the outer end surface of the installation groove 121 and is arranged in rolling contact with the sidewall of the vibration tub 21. According to the arrangement, the vibration barrel 21 can be supported, the movement of the vibration barrel 21 can be guided, and the friction force between the vibration barrel 21 and the guide mounting plate 12 can be reduced.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the utility model can be made, and equivalents and modifications of some features of the utility model can be made without departing from the spirit and scope of the utility model.

Claims

1. A raw material dissolving device for preparing a serum-free cell culture medium comprises: an eccentric shaft (117) drive assembly; it is characterized by comprising: a vibration barrel (21), a liquid transferring barrel (22), a guide mounting plate (12), a bracket and a hose pump (313); the middle part of the guide mounting plate (12) is provided with a mounting hole, and a plurality of sliding rollers (133) are mounted on the inner side wall of the guide mounting plate;

the vibration barrel (21) is inserted into a mounting hole formed in the guide mounting plate (12), and the side wall of the vibration barrel (21) is in rolling contact with the sliding roller (133); a bracket is arranged on the bottom surface of the guide mounting plate (12); the eccentric shaft (117) driving component is arranged below the bracket and is in driving connection with the bottom surface of the vibration barrel (21); the eccentric shaft (117) driving component drives the vibration barrel (21) to move up and down along the central axis of the guide mounting plate (12);

a liquid transferring barrel (22) is arranged in the vibration barrel (21); a threaded cover (221) is arranged on the top surface of the liquid transferring barrel (22); the top surface of the threaded cover (221) is provided with a through hole (224); the upper cover of the top surface of the vibration barrel (21) is provided with a flange cover (23); the flange cover (23) is provided with a liquid inlet pipe (212); the liquid inlet pipe (212) is inserted in the through hole (224) and is communicated with a liquid outlet end pipeline of the hose pump (313).

2. The serum-free cell culture medium preparation starting material lysing device according to claim 1, wherein the eccentric shaft (117) driving assembly comprises: the device comprises a driving motor (10), a coupling (11), a bearing seat (112), a rotating shaft (113), an eccentric shaft (117) and a hinge piece; the driving motor (10) is in driving connection with the rotating shaft (113) through a coupling (11); the rotating shaft (113) is rotatably arranged in the bearing seat (112); the eccentric shaft (117) is installed on the distal end surface of the rotating shaft (113) and connected with the vibration tub (21) through a hinge.

3. The serum-free cell culture medium preparation material dissolution device according to claim 2, comprising: a connecting rod hinge; the link hinge includes: a connecting rod (115), a hinge shaft (126) and a driving rod (114); one end of the connecting rod (115) is sleeved on the eccentric shaft (117); the other end of the connecting rod (115) is hinged with the driving rod (114) through a hinge shaft (126).

4. The serum-free cell culture medium preparation material dissolution device according to claim 3, comprising: a guide support plate (116), a guide shaft seat (124), a sloping plate (125) and a cylinder seat (127); the guide support plate (116) is arranged on the end face of the bearing seat (112), a hole is formed in the guide support plate (116), and the guide shaft seat (124) is arranged in the hole; the driving rod (114) is inserted in the guide shaft seat (124); the other end of the driving rod (114) is connected with the bottom surface of the cylinder seat (127); a plurality of inclined plates (125) are arranged on the outer side wall of the other end of the driving rod (114) at intervals.

5. The serum-free cell culture medium preparation material dissolution device according to claim 1, comprising: a seal cover (211); the sealing cover (211) is arranged on the first end surface of the liquid inlet pipe (212) in a covering mode.

6. The serum-free cell culture medium preparation starting material dissolution device according to claim 1, wherein the pipetting barrel (22) comprises: the flange edge is clamped between the vibration barrel (21) and the flange cover (23); the threaded cover (221) is inserted in the barrel body and is in threaded connection with the inner side wall of the upper part of the barrel body; the top surface of the threaded cover (221) is provided with a handle, so that the liquid transferring barrel (22) can be opened conveniently through the handle.