CN218620861U

CN218620861U - Cell culture mixing liquid feeding device

Info

Publication number: CN218620861U
Application number: CN202222312333.0U
Authority: CN
Inventors: 马秀玲; 李逸飞; 于雅洁; 张媛媛; 孙圣; 张连喜; 陈会明; 李海山
Original assignee: Cast Tianjin Inspection and Testing Co Ltd
Current assignee: Cast Tianjin Inspection and Testing Co Ltd
Priority date: 2022-08-31
Filing date: 2022-08-31
Publication date: 2023-03-14
Anticipated expiration: 2032-08-31

Abstract

The utility model provides a cell culture mixing liquid adding device, which comprises a base, a mixing mechanism and a liquid adding mechanism, wherein the mixing mechanism is positioned on the base, the liquid adding mechanism is positioned above the mixing mechanism and matched with the mixing mechanism, the mixing mechanism is provided with a fixed seat, and a culture bottle is positioned on the fixed seat; the mixing mechanism comprises a supporting seat, a mixing frame, a rotating shaft, a mixing plate and a servo motor, wherein the supporting seat is positioned on the base, a mixing groove is formed in the supporting seat, and the mixing frame is positioned on the mixing groove. A cell culture mixing liquid feeding device be provided with liquid feeding mechanism and mixing mechanism, carry out the liquid feeding to the blake bottle earlier, then carry out the mixing, realized batch liquid feeding mixing, reduced manual operation, improved experimental efficiency.

Description

Cell culture mixing liquid feeding device

Technical Field

The utility model belongs to the biochemistry field especially relates to a cell culture mixing liquid feeding device.

Background

Various operations such as bottle rotating, liquid adding, bagging, back transfusion and the like are required in the cell culture process, and the operations are generally processed by operators. The liquid adding is a conventional operation in the cell culture process, a large amount of fresh culture medium is often required to be supplemented in the cell culture process to meet the nutrient components necessary for cell growth, at present, most of the liquid adding is carried out by adopting an electric liquid-transferring gun and then manual mixing is carried out, so that the workload of operators is greatly improved.

Utility model patent No. 201921135710.X discloses a fluid infusion device that cell culture used, crosses liquid feeding section of thick bamboo and adds the culture solution to the blake bottle, need not to open the interpolation that the blake bottle can realize the culture solution, effectively reduces the contaminated probability of cell. Although the device can realize the liquid adding operation, the operator needs to shake and mix the bottle body after the liquid adding operation, which cannot be realized by the patent.

Disclosure of Invention

In view of this, the utility model discloses aim at overcoming the defect among the prior art, provide a cell culture mixing liquid feeding device.

In order to achieve the above purpose, the technical scheme of the utility model is realized as follows:

a cell culture mixing and liquid adding device comprises a base, a mixing mechanism and a liquid adding mechanism, wherein the mixing mechanism is positioned on the base, the liquid adding mechanism is positioned above the mixing mechanism and matched with the mixing mechanism, a fixed seat is arranged on the mixing mechanism, and a culture bottle is positioned on the fixed seat;

mixing mechanism include supporting seat, mixing frame, pivot, mixing board, servo motor, the supporting seat be located the base on, the supporting seat on be equipped with the mixing groove, the mixing frame be located the mixing groove on, the one end of pivot with pass the mixing frame with servo motor link to each other, the pivot on be provided with the mixing board, the top both sides of mixing board all be provided with a plurality of mixing pieces. Servo motor drives the pivot and carries out just reversing, and the pivot drives the mixing board and sways, and the mixing piece promotes the mixing frame after the swing and swings to drive the blake bottle on the fixing base and swing, thereby carry out the mixing operation. The fixing seat is provided with a plurality of clamping pieces for clamping the culture bottle.

Further, the mixing groove is of an arc-shaped structure, and the bottom of the mixing frame is of a semicircular structure. The blending frame swings on the blending groove after being driven by the blending block, and the blending groove is similar to a semi-elliptical structure and is suitable for swinging of the blending frame in a large angle.

Furthermore, the rotating shaft is rotatably connected with the blending frame, and a heavy hammer is arranged at the bottom of the blending frame. The weight can make the mixing frame keep vertical stable state when static, makes the blake bottle keep stable, is convenient for carry out the liquid feeding to the blake bottle.

Furthermore, the outer side of the blending block is of an arc structure. The mixing block can be contacted with the mixing frame at different positions.

Furthermore, a bracket is arranged on the base, and the liquid adding mechanism is fixed on the bracket.

Further, liquid feeding mechanism include liquid reserve tank, support frame, discharge pipe way, trunk line, the support frame be fixed in the support on, the liquid reserve tank be located the support frame on, discharge pipe way one end with the liquid reserve tank link to each other, the other end with the trunk line link to each other, the trunk line on be provided with a plurality of leakage fluid nipples, the leakage fluid nipples be located the top of the blake bottle that corresponds.

Furthermore, a flow valve is arranged on the discharge pipeline. The liquid adding quantity is controlled through a flow valve, and synchronous liquid adding of a plurality of culture bottles is realized.

Furthermore, the main pipeline is connected with the supporting frame through a fixing frame. Used for fixing the liquid adding mechanism.

Compared with the prior art, the utility model discloses following advantage has:

a cell culture mixing liquid feeding device be provided with liquid feeding mechanism and mixing mechanism, carry out the liquid feeding to the blake bottle earlier, then carry out the mixing, realized batch liquid feeding mixing, reduced manual operation, improved experimental efficiency.

Drawings

The accompanying drawings, which form a part hereof, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without undue limitation. In the drawings:

FIG. 1 is a schematic view of a cell culture mixing and feeding device according to an embodiment of the present invention;

fig. 2 is a schematic view of a static state of a blending mechanism according to an embodiment of the present invention;

fig. 3 is a schematic diagram of a blending state of a blending mechanism according to an embodiment of the present invention.

Description of reference numerals:

1. a base; 2. a blending mechanism; 3. a liquid adding mechanism; 4. a fixed seat; 5. a culture bottle; 11. a support; 21. a supporting base; 22. a blending frame; 23. a rotating shaft; 24. uniformly mixing the plates; 25. uniformly mixing blocks; 26. a servo motor; 27. a mixing tank; 28. a weight; 31. a liquid storage tank; 32. a support frame; 33. a discharge conduit; 34. a flow valve; 35. a fixed mount; 36. a main pipeline; 37. a liquid discharge nozzle.

Detailed Description

It should be noted that, in the case of no conflict, the embodiments and features of the embodiments of the present invention may be combined with each other.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

As shown in fig. 1-3, a cell culture mixing and liquid adding device comprises a base 1, a mixing mechanism 2 and a liquid adding mechanism 3, wherein the mixing mechanism 2 is positioned on the base 1, the liquid adding mechanism 3 is positioned above the mixing mechanism 2 and is matched with the mixing mechanism 2, a fixed seat 4 is arranged on the mixing mechanism 2, and a culture bottle 5 is positioned on the fixed seat 4;

mixing mechanism 2 include supporting seat 21, mixing frame 22, pivot 23, mixing board 24, servo motor 26, supporting seat 21 be located base 1 on, supporting seat 21 on be equipped with mixing groove 27, mixing frame 22 be located mixing groove 27 on, the one end of pivot 23 with pass mixing frame 22 with servo motor 26 link to each other, pivot 23 on be provided with mixing board 24, mixing board 24's top both sides all be provided with a plurality of mixing piece 25. The servo motor 26 drives the rotating shaft 23 to rotate positively and negatively, the rotating shaft 23 drives the blending plate 24 to swing, and the blending block 25 swings to push the blending rack 22 to swing so as to drive the culture bottles 5 on the fixing seat 4 to swing, thereby performing blending operation. Be provided with a plurality of holders on the fixing base 4, carry out the centre gripping to blake bottle 5.

The blending groove 27 is of an arc-shaped structure, and the bottom of the blending frame 22 is of a semicircular structure. The blending rack 22 swings on the blending groove 27 after being driven by the blending block 25, and the blending groove 27 is similar to a semi-elliptical structure and is suitable for swinging of the blending rack 22 in a large angle.

The rotating shaft 23 is rotatably connected with the blending rack 22, and a heavy hammer 28 is arranged at the bottom of the blending rack 22. The weight 28 can keep the mixing rack 22 in a stable vertical state when the mixing rack is static, so that the culture bottle 5 can be kept stable, and the culture bottle 5 can be conveniently filled with liquid. The outer side of the blending block 25 is of an arc structure. Facilitating contact of the blending block 25 with the blending rack 22 at various locations. The rotating shaft 23 is rotatably connected with the blending frame 22 through a bearing. The rotation of the servo motor 26 is changed into the driving of the mixing block 25, so that the shaking of the mixing frame 22 is more moderate, and the damage to cells is prevented.

The base 1 is provided with a bracket 11, and the liquid adding mechanism 3 is fixed on the bracket 11. Liquid feeding mechanism 3 include liquid reserve tank 31, support frame 32, discharge tube way 33, trunk line 36, support frame 32 be fixed in support 11 on, liquid reserve tank 31 be located support frame 32 on, discharge tube way 33 one end with liquid reserve tank 31 link to each other, the other end with trunk line 36 link to each other, trunk line 36 on be provided with 8 leakage fluid connectors 37, leakage fluid connectors 37 be located the top of corresponding blake bottle 5. The discharge pipe 33 is provided with a flow valve 34. The flow valve 34 is used for controlling the liquid adding amount, so that synchronous liquid adding of a plurality of culture bottles 5 is realized. The main pipeline 36 is connected with the support frame 32 through a fixing frame 35. For fixing the liquid adding mechanism 3.

The implementation process comprises the following steps:

place blake bottle 5 on the holder of fixing base 4, then open flow valve 34, liquid in the liquid reserve tank 31 flows into on trunk line 36 through discharge pipe 33, then flow into blake bottle 5 through flowing back mouth 37, accomplish the liquid feeding to blake bottle 5, at this moment, mixing frame 22 is because the effect of weight 28, the state of maintaining vertical stewing, then can open servo motor 26, servo motor 26 drives the axis of rotation and carries out corotation and reversal, thereby it rotates to have driven mixing board 24, and then be located the mixing piece 25 contact mixing frame 22 at mixing board 24 top, the arc of supporting seat 21 mixes even groove 27 and the semicircle form bottom of mixing frame 22 at this moment and cooperatees, can realize promoting the positive and negative slope on mixing frame 22 upper portion smoothly, thereby realize the mixing.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a cell culture mixing liquid feeding device which characterized in that: the liquid adding mechanism is positioned above the blending mechanism and matched with the blending mechanism, a fixed seat is arranged on the blending mechanism, and a culture bottle is positioned on the fixed seat;

mixing mechanism include supporting seat, mixing frame, pivot, mixing board, servo motor, the supporting seat be located the base on, the supporting seat on be equipped with the mixing groove, the mixing frame be located the mixing groove on, the one end of pivot with pass the mixing frame with servo motor link to each other, the pivot on be provided with the mixing board, the top both sides of mixing board all be provided with a plurality of mixing pieces.

2. The cell culture mixing and feeding device of claim 1, which is characterized in that: the mixing groove is of an arc-shaped structure, and the bottom of the mixing frame is of a semicircular structure.

3. The cell culture mixing and feeding device according to claim 2, characterized in that: the rotating shaft is rotatably connected with the blending frame, and a heavy hammer is arranged at the bottom of the blending frame.

4. The cell culture mixing and feeding device of claim 3, which is characterized in that: the outer side of the blending block is of an arc structure.

5. The cell culture mixing and feeding device according to claim 1, which is characterized in that: the base is provided with a bracket, and the liquid adding mechanism is fixed on the bracket.

6. The cell culture mixing and feeding device according to claim 5, characterized in that: liquid feeding mechanism include liquid reserve tank, support frame, exhaust duct, trunk line, the support frame be fixed in the support on, the liquid reserve tank be located the support frame on, exhaust duct one end with the liquid reserve tank link to each other, the other end with the trunk line link to each other, the trunk line on be provided with a plurality of leakage fluid nipples, the leakage fluid nipples be located the top of the blake bottle that corresponds.

7. The cell culture mixing and feeding device according to claim 6, characterized in that: and a flow valve is arranged on the discharge pipeline.

8. The cell culture mixing and feeding device of claim 6, which is characterized in that: the main pipeline pass through the mount with the support frame link to each other.