CN207362255U - A kind of attached cell blake bottle - Google Patents
A kind of attached cell blake bottle Download PDFInfo
- Publication number
- CN207362255U CN207362255U CN201721341027.2U CN201721341027U CN207362255U CN 207362255 U CN207362255 U CN 207362255U CN 201721341027 U CN201721341027 U CN 201721341027U CN 207362255 U CN207362255 U CN 207362255U
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- bottle
- blake bottle
- recoil
- stent
- commissioning pipe
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Abstract
The utility model discloses a kind of attached cell blake bottle, including body, and the bottle cap and bottom of bottle being threadedly coupled with body, bottom of bottle internal activity is connected with filter plate and stent, and stent is arranged on filter plate bottom, recoil nozzle is installed with stent, recoil nozzle connection recoil water pipe, the filtered plate of water pipe that recoils connects exterior tap through bottle cap, and by being wound with commissioning pipe at body, commissioning pipe is fixed on inside body recoil water pipe in the shape of a spiral；The utility model is passed through nutriment etc. by continuing into commissioning pipe or being interrupted, so as to adjust the concentration of nutriment in blake bottle in time, to maintain the required condition of culture of cell growth, the nutrient concentrations regulation and control solved in prior art blake bottle are constant, the problems such as complicated easy to pollute, spiral helicine commissioning pipe more uniformly feeds intake in blake bottle, improves cell bond area, is suitable for concentration cultivation；So that liquid feeding is convenient, work efficiency is improved.
Description
Technical field
Attached cell culture device technical field is the utility model is related to, is specially a kind of attached cell blake bottle.
Background technology
In biotechnology, cell culture is an essential process.Cell culture is usually to put cell
Enter blake bottle, then be put into incubator and cultivated.
But current Tissue Culture Flask, in laboratory Batch Culture attached cell, is collected in the prevalence of a problem
During cell, when taking out cell from Tissue Culture Flask bottleneck, since bottleneck is smaller, culture cell is difficult to extract, adherent cell collecting
Efficiency it is low, and bottle easily produce extraction dead angle, cause the waste of a large amount of attached cells, while existing Tissue Culture Flask
In cleaning process, cleaning dead angle can be also produced, and its it is inconvenient.
The content of the invention
The technical problems to be solved in the utility model is the defects of overcoming existing culture cell to be difficult to extract and clean, there is provided
A kind of attached cell blake bottle, so as to solve the above problems.
In order to solve the above-mentioned technical problem, the utility model provides following technical solution：
A kind of attached cell blake bottle of the utility model, including body, and the bottle cap and bottom of bottle being threadedly coupled with body,
Bottom of bottle internal activity is connected with filter plate and stent, and stent is arranged on filter plate bottom, recoil nozzle is installed with stent,
Recoil nozzle connection recoil water pipe, and the filtered plate of recoil water pipe connects exterior tap through bottle cap, and recoil water pipe passes through body
Place is wound with commissioning pipe, and commissioning pipe is fixed on inside body in the shape of a spiral.
As a kind of optimal technical scheme of the utility model, body, bottle cap and bottom of bottle junction are provided with sealing ring, close
Seal suit is fixed on body opening.
As a kind of optimal technical scheme of the utility model, commissioning pipe charging port is arranged on body, in feed end
The connector being connected with that can add the exterior liquid adding tube of nutriment is installed at mouthful.
As a kind of optimal technical scheme of the utility model, commissioning pipe is made of the permselective membrane of loose structure
Permeability pipeline.
As a kind of optimal technical scheme of the utility model, body is made of double transparent material.
The beneficial effect that the utility model is reached is：1st, the utility model into commissioning pipe 4 by continuing or open close
Enter nutriment etc., so as to adjust the concentration of nutriment in blake bottle in time, to maintain the required training of cell growth
Support condition, solve in prior art blake bottle nutrient concentrations regulation and control it is constant, it is complicated easy to pollute the problems such as, helical form
Commissioning pipe 4 more uniformly feed intake in blake bottle, improve cell bond area, be suitable for concentration cultivation；At the same time
The Toxic Metabolites produced in the discharge cell growth reproductive process that logical commissioning pipe 4 can also be selective, are conducive to the growth of cell
Propagation；Connector 5 is set to realize quick connection so that liquid feeding is convenient, improves work efficiency；
2nd, the utility model is mainly used for the culture of attached cell, and cell is grown in bottle wall, is fallen into bottom of bottle 6 and is filtered
Material on plate 7 is substantially waste material, and waste material is deposited on filter plate, filter plate 7 is rinsed by back purge system, mistake
Filter plate 7 is flexibly connected with bottom of bottle 6, convenient cleaning；Bottom of bottle 6 sets foot guard layer and shatter-resistant jacket layer, falls in culture bottle body
Or its extent of damage is reduced after dropping, better ensure the safety of culture bottle body；
3rd, blake bottle is made of double transparent material, is not only convenient for the situation that people visually observes cell culture, moreover it is possible to subtract
Few influence of the external environment to cell culture.
Brief description of the drawings
Attached drawing is used to provide a further understanding of the present invention, and a part for constitution instruction, with this practicality
New embodiment is used to explain the utility model together, does not form the limitation to the utility model.
In the accompanying drawings：
Fig. 1 is the utility model structure diagram；
Figure label：1st, body；2nd, bottle cap；3rd, sealing ring；4th, commissioning pipe；5th, connector；6th, bottom of bottle；7th, filter plate；8、
Stent；9th, recoil nozzle；10th, recoil water pipe.
Embodiment
The preferred embodiment of the utility model is illustrated below in conjunction with attached drawing, it will be appreciated that described herein excellent
Select embodiment to be only used for describing and explaining the present invention, be not used to limit the utility model.
, it is necessary to explanation in the description of the utility model, the instruction such as term " vertical ", " on ", " under ", " level "
Orientation or position relationship are based on orientation shown in the drawings or position relationship, are for only for ease of description the utility model and letter
Change description, rather than instruction or imply signified device or element must have specific orientation, with specific azimuth configuration and
Operation, therefore it is not intended that limitation to the utility model.
In the description of the utility model, it is also necessary to which explanation, unless otherwise clearly defined and limited, term " are set
Put ", " installation ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected,
Or it is integrally connected；Can mechanically connect or be electrically connected；It can be directly connected, intermediary can also be passed through
It is indirectly connected, can is the connection inside two elements.For the ordinary skill in the art, can be according to specific
Situation understands concrete meaning of the above-mentioned term in the utility model.
Embodiment：As shown in Figure 1, the utility model provides a kind of attached cell blake bottle, including body 1, and with bottle
The bottle cap 2 and bottom of bottle 6,6 internal activity of bottom of bottle that body 1 is threadedly coupled are connected with filter plate 7 and stent 8, and stent 8 is arranged on filter plate
7 bottoms, recoil nozzle 9, the recoil connection recoil water pipe 10 of nozzle 9 are installed with stent 8, and recoil water pipe 10 passes through filter plate 7
Exterior tap is connected through bottle cap 2, for recoil water pipe 10 by being wound with commissioning pipe 4 at body 1, commissioning pipe 4 is solid in the shape of a spiral
It is scheduled on inside body 1.
Body 1, bottle cap 2 and 6 junction of bottom of bottle are provided with sealing ring 3, and the suit of sealing ring 3 is fixed on 1 opening of body；By
All it is opening in 1 both ends of body, in order to ensure that whole blake bottle is in the environment of a sealing, then needs to own in blake bottle
Interface carries out encapsulation process, is to ensure that bottle cap 2 and bottom of bottle 6 are freely rotatable at body 1 using sealing ring.
4 charging port of commissioning pipe is arranged on body 1, and the outside with that can add nutriment is provided with charging port
The connector 5 that liquid adding tube is connected；Commissioning pipe 4 is permeability pipeline made of the permselective membrane of loose structure；Trained in cell
During supporting, nutriment etc. is passed through by continuing into commissioning pipe 4 or being interrupted, so as to adjust nutrition in blake bottle in time
The concentration of material, to maintain the required condition of culture of cell growth, solves the nutrient concentrations in prior art blake bottle
Regulate and control it is constant, it is complicated easy to pollute the problems such as, while logical commissioning pipe 4 can also be in the discharge cell growth reproductive process of selectivity
The Toxic Metabolites of generation, are conducive to the growing multiplication of cell；Connector 5 is set to realize quick connection so that liquid feeding is convenient, carries
High workload efficiency.
Body 1 is made of double transparent material, and the feelings that people visually observes cell culture are easy for using transparent material
Condition, and set using double layer material, the effect of insulation is can reach, reduces influence of the external environment to cell culture, when extraneous ring
During the cataclysm of border, double layer material, gives cell one cushion space.
Operation principle：Using the utility model blake bottle when, bottom of bottle 6 is threadedly secured on body 1 first, it is then logical
Cross bottle cap 2 and the cell for needing to cultivate is injected into blake bottle；In cell cultivation process, by into commissioning pipe 4 continue or
It is open close enter nutriment etc., so as to adjust the concentration of nutriment in blake bottle in time, required for maintaining cell growth
Condition of culture, solve in prior art blake bottle nutrient concentrations regulation and control it is constant, it is complicated easy to pollute the problems such as, together
When lead to commissioning pipe 4 can also the Toxic Metabolites that produce in the discharge cell growth reproductive process of selectivity, be conducive to the life of cell
Long propagation；In prolonged cell culture, cell metabolism produces certain waste material and is deposited on filter plate 7, this is logical
Cross recoil water pipe 10 and recoil nozzle 9 is rinsed to filter plate 7, unnecessary waste material is fallen into the bottom of bottom of bottle 6, and be discharged into bottom of bottle 6
In the filter pocket of bottom, after cell culture, recoil water pipe 10 and the recoil flushing body 1 of nozzle 9 repeatedly；The waste water of deposition leads to
Cross bottom of bottle 6 to discharge, bottom of bottle 6 is flexibly connected with 1 screw thread of body.
Finally it should be noted that：It these are only the preferred embodiment of the utility model, be not limited to this practicality
It is new, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art,
It can still modify the technical solution described in foregoing embodiments, or which part technical characteristic is carried out etc.
With replacement.Where within the spirit and principles of the present invention, any modification, equivalent replacement, improvement and so on, should all include
Within the scope of protection of the utility model.
Claims (5)
1. a kind of attached cell blake bottle, it is characterised in that including body（1）, and and body（1）The bottle cap of threaded connection
（2）And bottom of bottle（6）, bottom of bottle（6）Internal activity is connected with filter plate（7）And stent（8）, stent（8）It is arranged on filter plate（7）Bottom
Portion, stent（8）On be installed with recoil nozzle（9）, recoil nozzle（9）Connection recoil water pipe（10）, recoil water pipe（10）Through
Filter plate（7）Through bottle cap（2）The exterior tap of connection, recoil water pipe（10）By body（1）Place is wound with commissioning pipe（4）,
Commissioning pipe（4）Body is fixed in the shape of a spiral（1）It is internal.
A kind of 2. attached cell blake bottle according to claim 1, it is characterised in that body（1）, bottle cap（2）And bottom of bottle
（6）Junction is provided with sealing ring（3）, sealing ring（3）Suit is fixed on body（1）Opening.
A kind of 3. attached cell blake bottle according to claim 1, it is characterised in that commissioning pipe（4）Charging port is set
In body（1）On, the connector being connected with that can add the exterior liquid adding tube of nutriment is installed at charging port（5）.
A kind of 4. attached cell blake bottle according to claim 1, it is characterised in that commissioning pipe（4）It is loose structure
Permeability pipeline made of permselective membrane.
A kind of 5. attached cell blake bottle according to claim 1, it is characterised in that body（1）It is by Double-layered transparent material
Made of material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721341027.2U CN207362255U (en) | 2017-10-18 | 2017-10-18 | A kind of attached cell blake bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721341027.2U CN207362255U (en) | 2017-10-18 | 2017-10-18 | A kind of attached cell blake bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207362255U true CN207362255U (en) | 2018-05-15 |
Family
ID=62420569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721341027.2U Active CN207362255U (en) | 2017-10-18 | 2017-10-18 | A kind of attached cell blake bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207362255U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111575149A (en) * | 2020-05-09 | 2020-08-25 | 潘齐辉 | Culture device and culture method for slow-release aerobic strains |
-
2017
- 2017-10-18 CN CN201721341027.2U patent/CN207362255U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111575149A (en) * | 2020-05-09 | 2020-08-25 | 潘齐辉 | Culture device and culture method for slow-release aerobic strains |
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