CN204185470U - Cell perfusion water level adjusting device - Google Patents
Cell perfusion water level adjusting device Download PDFInfo
- Publication number
- CN204185470U CN204185470U CN201420559329.7U CN201420559329U CN204185470U CN 204185470 U CN204185470 U CN 204185470U CN 201420559329 U CN201420559329 U CN 201420559329U CN 204185470 U CN204185470 U CN 204185470U
- Authority
- CN
- China
- Prior art keywords
- perfusion
- supporting seat
- mounting hole
- spring mounting
- pillar stiffener
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000010412 perfusion Effects 0.000 title claims abstract description 65
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 16
- 239000003351 stiffener Substances 0.000 claims description 25
- 239000000839 emulsion Substances 0.000 claims description 12
- 125000003003 spiro group Chemical group 0.000 claims description 7
- 238000005452 bending Methods 0.000 claims description 4
- 238000010276 construction Methods 0.000 claims description 4
- 238000001802 infusion Methods 0.000 claims description 4
- 238000007493 shaping process Methods 0.000 claims description 3
- 238000013519 translation Methods 0.000 claims description 3
- 238000010586 diagram Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell perfusion water level adjusting device, which comprises a supporting seat, wherein the bottom of the supporting seat is provided with a strong magnet used for adsorbing the supporting seat on the disc surface of an observation microscope, and the top of the supporting seat is provided with supporting columns which are vertically arranged; a screw section is arranged on the outer circle of the support column, and an adjusting nut is arranged on the screw section; a spring mounting hole is formed in the supporting column along the axial direction of the supporting column, and a return spring is arranged in the spring mounting hole; the side wall of the support column is symmetrically provided with guide grooves communicated with the spring mounting hole along the axial direction of the side wall, perfusion tubes horizontally arranged are inserted into the spring mounting hole and the guide grooves on the two sides of the spring mounting hole, and the perfusion tubes can be clamped between the adjusting nut and the reset spring in a vertically-moving mode. The perfusion device is suitable for culture dishes of various specifications, can provide stable perfusion and effectively improve the efficiency of cell perfusion, is simple to operate, simple and convenient to use, easy to install and disassemble, and convenient to clean, and perfusion is not easy to block.
Description
Technical field
The utility model relates to cell perfusion supplementary unit, specifically refer to a kind of can the cell perfusion water level control apparatus of inhalent siphon and/or rising pipe position in fixed cell perfusing course.
Background technology
It is after cell is added reactor together with substratum that perfusion type is cultivated, and in cell enlargement and product forming process, is constantly taken out by partial condition substratum, continuously pours into new substratum again simultaneously.The advantage that perfusion type is cultivated is: 1, cell closure works system can make cell or enzyme be retained in reactor, maintains higher cell density, generally can reach 10
10cells/mL, thus the output improving product significantly; 2, continuous irrigation streaming system, be in making cytotostatic in good nutrient environment, detrimental metabolic refuse concentration buildup is lower; 3, speed of reaction easily controls, and culture cycle is longer, can boost productivity, and the target product rate of recovery is high; 4, product residence time in reactor is short, preserves under can being recovered to low temperature in time, is conducive to the activity keeping product.
Usually need to carry out cell perfusion in the processs of the test such as physiology, perfusion mode is previously directly fixed in special cell perfusion groove by perfusion tube, but the perfusion groove complex structure that this mode makes, perfusion groove easily blocks, and is unfavorable for cleaning and installs simultaneously.In addition, when carrying out drug perfusion experiment, the entrance and exit of perfusate is a key link in perfusing course, in existing perfusion device, all that the entrance and exit of perfusate is fixed in special cell plate, change cell must cell plate also be changed, for experiment brings very large inconvenience at every turn simultaneously.
Summary of the invention
The purpose of this utility model to provide exactly a kind of structure simple, be connected compact, operate cell perfusion water level control apparatus easily, inhalent siphon and the/rising pipe position of perfusate fast can be regulated by this device, at entrance and exit place, mineralization pressure is poor, thus can form stable perfusion system.
For achieving the above object, cell perfusion water level control apparatus designed by the utility model, comprise supporting seat, its special character is: be provided with the strong magnets for being adsorbed in microscope for observing card bottom described supporting seat, and described supporting seat top is provided with vertically arranged pillar stiffener; Described pillar stiffener cylindrical is provided with spiro rod section, and described spiro rod section is provided with setting nut; Described pillar stiffener is inner is axially provided with spring mounting hole along it, is provided with return spring in described spring mounting hole; Described pillar stiffener sidewall offers the guide path through with spring mounting hole along its axial symmetry, be interspersed with horizontally disposed perfusion tube in the guide path of described spring mounting hole and both sides thereof, described perfusion tube can be clamped between setting nut and return spring upper and lower translation.
Principle of work of the present utility model is such: arrange a cell perfusion water level control apparatus in the ingress of Tissue Culture Dish, this device by the utilizing powerful magnet for adsorption on its supporting seat in microscope for observing card, after liquid configures, by the entrance of perfusion tube access Tissue Culture Dish, utilize the cooperation of the setting nut on its pillar stiffener and return spring to adjust the position of perfusion tube, perfusate will be flowed in culture dish with the height of setting.Meanwhile, arrange another cell perfusion water level control apparatus in the exit of Tissue Culture Dish, drawn by perfusate by its perfusion tube, this perfusion tube can connect vacuum extractor usually.Regulate the height between two mouths, just can form stable perfusion system.
Preferably, described supporting seat and pillar stiffener are shaping by two diameters concentric cylinder integral construction not etc., described supporting seat external diameter is greater than pillar stiffener external diameter, the spring mounting hole of described pillar stiffener inside extends to bottom supporting seat always and bottom, hole is provided with screw thread, be provided with magnet bottom described supporting seat and counterbore is installed, described strong magnets is embedded in magnet and installs in counterbore, and is fixed by the holding bolt matched with spring mounting hole bottom screw thread.Like this, its one-piece construction is simple and practical, and supporting seat is fixed efficient and convenient, and perfusion tube altitude mixture control nimbly and freely, effectively can improve the efficiency of cell perfusion.
Further, described supporting seat external diameter is 1.5 ~ 2 times of pillar stiffener external diameter.Like this, adopt up-small and down-big gradient-structure, be conducive to cell perfusion water level control apparatus absorption location firm, guarantee working stability.
Further, one end of described perfusion tube is provided with downward-sloping obtuse angle bending section.Like this, be convenient to it be inserted in Tissue Culture Dish.
Again further, the other end of described perfusion tube is provided with emulsion tube joint, and described emulsion tube joint is connected with infusion pump by emulsion tube.Like this, the emulsion tube changing different size parameter is convenient to, to adapt to different perfusion needs.
Advantage of the present utility model is mainly reflected in following several respects:
One, by the cooperation of setting nut and return spring, fast can realize the adjustment of perfusion tube height, be applicable to the Tissue Culture Dish of various different size.
Its two, by the strong magnets on supporting seat, whole device is absorbed and fixed in microscope for observing card, while stable perfusion is provided, effectively can improves the efficiency of cell perfusion.
Its three, smart structural design, easy to use, and be easy to install, dismounting and cleaning, perfusate not easily blocks.
Its four, use this device coordinate Tissue Culture Dish, cell perfusion can be directly used in, make patch clamp, intracellular Ca2+ mensuration etc. study become very convenient.
Accompanying drawing explanation
Fig. 1 is a kind of main TV structure schematic diagram of cell perfusion water level control apparatus;
Fig. 2 is the sectional structure schematic diagram of Fig. 1;
Fig. 3 is the left TV structure schematic diagram of Fig. 1;
Fig. 4 is the A-A sectional structure schematic diagram in Fig. 1.
In figure: holding bolt 1; Strong magnets 2; Supporting seat 3 (wherein: magnet installs counterbore 3a); Return spring 4; Emulsion tube joint 5; Perfusion tube 6 (wherein: obtuse angle bending section 6b); Setting nut 7; Pillar stiffener 8 (wherein: spiro rod section 8a, spring mounting hole 8b, guide path 8c).
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in further detail, but this embodiment should not be construed restriction of the present utility model.
Cell perfusion water level control apparatus shown in figure, comprises supporting seat 3 and the pillar stiffener 8 being arranged vertically on its top.Particularly, supporting seat 3 and pillar stiffener 8 are shaping by two diameters concentric cylinder integral construction not etc., and supporting seat 3 external diameter is 1.5 ~ 2 times of pillar stiffener 8 external diameter, reliable to make it erect fixing-stable.
Pillar stiffener 8 excircle is provided with spiro rod section 8a, this spiro rod section 8a is provided with setting nut 7.Axially be provided with spring mounting hole 8b in pillar stiffener 8 inside along it, in this spring mounting hole 8a, be placed with return spring 4.The guide path 8c through with spring mounting hole 8b is offered along its axial symmetry at pillar stiffener 8 sidewall, be interspersed with horizontally disposed perfusion tube 6 in the guide path 8c of spring mounting hole 8b and both sides thereof, this perfusion tube 6 can be clamped between setting nut 7 and return spring 4 upper and lower translation.For the ease of being inserted in Tissue Culture Dish, one end of perfusion tube 6 is designed to downward-sloping obtuse angle bending section 6b.The other end of perfusion tube 6 is then connected with emulsion tube joint 5, and emulsion tube joint 5 is connected with infusion pump (not shown) by emulsion tube, for being introduced by perfusate.
Bottom supporting seat 3, be provided with magnet install counterbore 3a, magnet is installed in counterbore 3a and is equipped with strong magnets 2, for whole device is adsorbed on microscope for observing card.In the present embodiment, the magnet that the spring mounting hole 8b of pillar stiffener 8 inside extends to bottom supporting seat 3 always installs counterbore 3a place, and bottom, hole is provided with screw thread, strong magnets 2 is fixed as one with supporting seat 3 by the holding bolt 1 matched with spring mounting hole 8b bottom screw thread.
During utility model works, two devices are absorbed and fixed in microscope for observing card by strong magnets 2 is stable, the perfusion tube 6 of two devices corresponds respectively to the entrance and exit of Tissue Culture Dish, setting nut 7 on the device of coarse adjustment ingress, open infusion pump, make perfusate flow into Tissue Culture Dish by emulsion tube joint 5, perfusion tube 6, then finely tune this setting nut 7, while observe the flow state of perfusate, finally determine the height of the perfusion tube 6 on the device of ingress.In like manner, regulate the height of perfusion tube 6 on the device of exit, and this perfusion tube 6 is connected with vacuum extractor, finally for Tissue Culture Dish provides stable perfusion.
The content be not described in detail in this specification sheets, belongs to the known prior art of those skilled in the art.
Claims (5)
1. a cell perfusion water level control apparatus, comprise supporting seat (3), it is characterized in that: described supporting seat (3) bottom is provided with the strong magnets (2) for being adsorbed in microscope for observing card, and described supporting seat (3) top is provided with vertically arranged pillar stiffener (8); Described pillar stiffener (8) cylindrical is provided with spiro rod section (8a), described spiro rod section (8a) is provided with setting nut (7); Described pillar stiffener (8) is inner is axially provided with spring mounting hole (8b) along it, is provided with return spring (4) in described spring mounting hole (8b); Described pillar stiffener (8) sidewall offers the guide path (8c) through with spring mounting hole (8b) along its axial symmetry, be interspersed with horizontally disposed perfusion tube (6) in the guide path (8c) of described spring mounting hole (8b) and both sides thereof, described perfusion tube (6) can be clamped between setting nut (7) and return spring (4) upper and lower translation.
2. cell perfusion water level control apparatus according to claim 1, it is characterized in that: described supporting seat (3) and pillar stiffener (8) are shaping by two diameters concentric cylinder integral construction not etc., described supporting seat (3) external diameter is greater than pillar stiffener (8) external diameter, the spring mounting hole (8b) of described pillar stiffener (8) inside extends to supporting seat (3) bottom always, and bottom, hole is provided with screw thread, described supporting seat (3) bottom is provided with magnet and installs counterbore (3a), described strong magnets (2) is embedded in magnet and installs in counterbore (3a), and fixed by the holding bolt (1) matched with spring mounting hole (8b) bottom screw thread.
3. cell perfusion water level control apparatus according to claim 2, is characterized in that: described supporting seat (3) external diameter is 1.5 ~ 2 times of pillar stiffener (8) external diameter.
4. the cell perfusion water level control apparatus according to claim 1 or 2 or 3, is characterized in that: one end of described perfusion tube (6) is provided with downward-sloping obtuse angle bending section (6b).
5. cell perfusion water level control apparatus according to claim 4, it is characterized in that: the other end of described perfusion tube (6) is provided with emulsion tube joint (5), described emulsion tube joint (5) is connected with infusion pump by emulsion tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420559329.7U CN204185470U (en) | 2014-09-26 | 2014-09-26 | Cell perfusion water level adjusting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420559329.7U CN204185470U (en) | 2014-09-26 | 2014-09-26 | Cell perfusion water level adjusting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204185470U true CN204185470U (en) | 2015-03-04 |
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ID=52617482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420559329.7U Expired - Fee Related CN204185470U (en) | 2014-09-26 | 2014-09-26 | Cell perfusion water level adjusting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204185470U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113406316A (en) * | 2021-06-17 | 2021-09-17 | 重庆医科大学附属儿童医院 | Electrophysiological patch clamp perfusion device |
-
2014
- 2014-09-26 CN CN201420559329.7U patent/CN204185470U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113406316A (en) * | 2021-06-17 | 2021-09-17 | 重庆医科大学附属儿童医院 | Electrophysiological patch clamp perfusion device |
| CN113406316B (en) * | 2021-06-17 | 2023-08-08 | 重庆医科大学附属儿童医院 | Electrophysiology patch clamp perfusion device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150304 Termination date: 20170926 |
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| CF01 | Termination of patent right due to non-payment of annual fee |