CN206396247U - Band automatically controls the new incubator of oscillating uniform device - Google Patents
Band automatically controls the new incubator of oscillating uniform device Download PDFInfo
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- CN206396247U CN206396247U CN201720041820.4U CN201720041820U CN206396247U CN 206396247 U CN206396247 U CN 206396247U CN 201720041820 U CN201720041820 U CN 201720041820U CN 206396247 U CN206396247 U CN 206396247U
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- rotating disk
- runing rest
- rotary shaft
- oscillating uniform
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Abstract
The utility model discloses the new incubator that a kind of band automatically controls oscillating uniform device, it includes casing, oscillating uniform device and timer, oscillating uniform device includes horizontal rotation apparatus, vertical rotating device and rocking equipment, second rotary shaft is rotatably provided on base, second rotary shaft one end is connected to horizontal rotatable platform, its other end is connected with the output shaft of the second motor, and the second motor is fixed on the bottom of base by electric machine support；Vertical rotating device is located at case intracavitary, runing rest is arranged on horizontal rotatable platform, first rotary shaft is rotatably provided on runing rest, first motor is fixedly installed in the hollow cavity of runing rest, the output shaft of first motor is connected with one end of the first rotary shaft, the other end of first rotary shaft is fixedly connected with rotating disk, and the circular hole for the centrifuge tube that can insert various different capabilities is provided with rotating disk；The utility model can realize that machine automatization control shakes up, and improve operating efficiency, raising and shake up effect, be adapted to the Batch Culture of cell.
Description
Technical field
The utility model is related to medical instruments field, it particularly relates to which a kind of be applied to cytogenetic can be achieved
Centrifuge tube dress culture medium automatic mixing and culture, can automatically control direction, speed and temperature compact conformation, it is easy to operate,
Avoid waste sample from wasting, improve operating efficiency, improving and shake up the band of effect and automatically control the new incubator of oscillating uniform device.
Background technology
Cell culture is most basic and the most frequently used experiment in cytogenetics field, wherein most widely used is people periphery
Blood lymphocyte culture.Traditional human peripheral lymphocyte culture is that the human peripheral blood lymphocytes that human blood is added into cillin bottle packaging are thin
In born of the same parents' culture medium, be put into common incubator carry out it is incubated, after culture 68-72 hour will inside cillin bottle it is cultured carefully
Dysuria with lower abdominal colic enters 15ml centrifuge tubes and is collected.Add culture as the application of some centrifuge tube patent new products changes traditional cillin bottle
The cultural method of case, and improve original cultural method and simplify culture flow：It is first by medium package in centrifugation
Guan Zhong, then blood directly added and cultivated in the culture medium for centrifuging tube packaging, blood is cultivated into 68-72 in centrifuge tube
It is collected by centrifugation after hour, eliminates the step of cell in cillin bottle is transferred to centrifuge tube, saves time, increase operation peace
Full property and the reliability of experiment.
Although cultivating the improvement of operating method more than, find in an experiment, the culture directly filled using centrifuge tube
Base carries out cell culture, and the amount that the amount meeting bottled culture medium in benzathine penicillin G of the lymphocyte finally harvested is cultivated will be lacked, though this
Analysis of experimental results is not influenceed, but the not result for being optimal.Found in follow-up Optimal Experimental, cause above-mentioned existing
As the reason for be mainly the difference of cell culturing space：Centrifuge tube capacity is 15ml, and cillin bottle capacity is 20ml, and due to centrifugation
Pipe is with the cast of cillin bottle different, and causing the cross section of centrifuge tube, to compare cillin bottle small by 50% or so, so as to cause cell to exist
Contact surface when being cultivated in centrifuge tube with air also relatively reduces 50%, adds high in centrifuge tube dress culture medium medium size lymphocyte
Degree is gathered in the conical pipe bottom of centrifuge tube, it can not be fully contacted with the culture medium in centrifuge tube, causes cell growth
And its propagation efficiency reduction, finally reduce the quantity of cell culture in centrifuge tube.
In order to solve problem above, found, the centrifuge tube placement for cultivating cell is provided with special by many experiments
Cultivated in the incubator of centrifuge tube shelf, and centrifuge tube is placed in horizontal by 15 DEG C of inclination angle on centrifuge tube shelf, can
To be effectively increased surface area of the culture medium with air contact, it is possible to resolve in the prior art because in centrifuge tube media surface area it is too small
And influence the shortcoming of centrifuge tube cell culture；In addition, mixing a centrifuge culture base by artificial every 24 hours, make lymphocyte
Fully mixed with culture medium and be conducive to the culture of lymphocyte to breed, it is possible to resolve lymphocyte aggregation is at the conical pipe bottom of centrifuge tube
The defect of culture medium can not fully be contacted.
The further improvement of operating method is cultivated even across more than, has caused centrifuge tube dress culture medium to carry out cell culture
The cell yield that is obtained of cell culture is carried out almost without significant difference with the bottled culture medium in XiLin, but in the reality of cell culture
Also had the following disadvantages in operation and application：1) because the structure of centrifuge tube shelf is general more complicated, volume is larger, it is occupied training
The larger space of case is supported, causes the space efficiency utilization of incubator low；2) traditional cell culture incubator does not possess machinery generally certainly
Shake even mixed function, it is necessary to which operating personnel mixed by hand, because the qualification of operating personnel is different and mixed
Even dynamics is difficult to grasp, and often results in centrifuge tube inner cell and mixes insufficient or the problems such as cause cell rupture, causes to shake up
Effect is unsatisfactory, so cause experimental result easily there is deviation, even result in final the failure of an experiment, easily cause manpower,
The waste of material resources and resource；3) carried out mixing a centrifuge culture base by artificial every 24 hours, cause needs weight daily
The trouble operated again, it is not only time-consuming and laborious, the working strength of staff is added, operating efficiency is reduced；By artificial
Also there is inconvenience in the mode shaken up manually, such as sometimes because it is busy forget mixing time, mixing time is just in morning sometimes
Morning, night or weekend, staff's inconvenience on and off duty etc. is caused, easily the mood of influence operating personnel, and because based on people
Sight factor is impacted to cell culture effect.
Therefore, in cell culture experiments, in the Batch Culture for particularly carrying out cell, a kind of compact conformation of urgent need invention,
Temperature control culture that is easy to operate, avoiding waste sample from wasting, improve operating efficiency, improving the timing automatic oscillating uniform device of band for shaking up effect
Case.
Utility model content
For more than deficiency, the utility model provide a kind of compact conformation, it is easy to operate, avoid waste sample waste, carry
The band that high workload efficiency, raising shake up effect automatically controls the new incubator of oscillating uniform device, and it includes that heat-insulated case can be closed
Body, the temperature control system being made up of heater, hygrosensor and controller, the casing are provided with case chamber, the heating
Device is arranged at case intracavitary with the hygrosensor, and the controller is arranged on casing；It also includes oscillating uniform device and fixed
When device, the oscillating uniform device include horizontal rotation apparatus and vertical rotating device, the horizontal rotation apparatus include base, second
Rotary shaft, the second motor and horizontal rotatable platform, the base are arranged on the bottom of the casing case chamber, second rotary shaft
It is rotatably provided on base, the case intracavitary of the casing is stretched into described second rotary shaft one end, and is connected to and is arranged at case chamber
Interior horizontal rotatable platform, the other end of second rotary shaft is rushed across base and is connected with the output shaft of second motor, institute
State the bottom that the second motor is fixedly mounted on the base by the electric machine support；
The vertical rotating device is arranged on the case intracavitary, the vertical rotating device include rotating disk, the first rotary shaft,
First motor and runing rest, the runing rest are arranged on the horizontal rotatable platform, and first rotary shaft is rotatable
It is arranged on the runing rest, first motor is fixedly installed in the hollow cavity of the runing rest, described first
The output shaft of motor is connected with one end of first rotary shaft, and the other end of first rotary shaft is fixed with the rotating disk to be connected
Connect, the circular hole of some centrifuge tubes for inserting various different capabilities is provided with the rotating disk；
The timer is used to be timed control to the working time of first motor and second motor, described
Timer is electrically connected with first motor with second motor, the controller and heater, hygrosensor, timing
Device, the first motor and the second motor are electrically connected with, with the temperature in control cabinet, the rotating speed of rotating disk, the steering of rotating disk and rotating disk
Timing mode.
In order to further realize the present invention, the oscillating uniform device also includes rocking equipment, and the rocking equipment includes waving
Pedestal, the 3rd motor, eccentric wheel and Swing Arm, the swaying base are arranged on the horizontal rotatable platform, the 3rd electricity
Machine is fixedly mounted on the lower portion of the swaying base, and the eccentric wheel is arranged on the power output shaft of the 3rd motor
On, one end of the Swing Arm is hinged on eccentric wheel, and the other end of the Swing Arm is hinged on the runing rest, described
The edge portion of swaying base is provided with a pair of circular pins, and the swaying base passes through a pair of circular pins and the runing rest
It is hinged.
In order to further realize the present invention, set in the hunting range that the runing rest is corresponded on the swaying base
The breach of arc is set to, to cause the runing rest to have a space mutually avoided in reciprocally swinging, it is to avoid wave base
Seat collides with runing rest.
In order to further realize the present invention, the runing rest is generally cylindrical, the runing rest axis and level
Line is set in 15 degree of angles.
In order to further realize the present invention, the rotating disk is in axis and plane-parallel, and the rotating disk is arranged at the case
The case intracavitary middle position of body.
The circular hole axially penetrated through along it, the number of the circular hole are provided with the present invention, the rotating disk in order to further realize
Amount is at least one.
In order to further realize the present invention, the rotating disk is discoid in thinner thickness, and the circular hole is evenly arranged in institute
State on the disk end face of rotating disk.
In order to further realize the present invention, the central axis of the circular hole intersects with the central axis of the rotating disk in 15 degree
Angle.
Centrifugation is prevented in order to further realize that the position for corresponding to each circular hole on the present invention, the rotating disk is additionally provided with
Elastic band or adapter that pipe comes off, to prevent centrifuge tube from out of circular hole shaking or taking off when being rotated with rotating disk
Fall.
It is easy to operating personnel to put the rotating disk for being inserted with centrifuge tube to further realize to be provided with the present invention, the casing
Enter or take out to be additionally provided with the input/output module being electrically connected with controller, display mould on the chamber door of case chamber, the casing
Block, so as to operating personnel can be inputted in input/output module setting temperature value, disc rotation speed, disk turn to, wave concussion
Amplitude and frequency values, and can show that the temperature value, disc rotation speed, disk of work at present turn to, wave shake in real time by display module
Swing amplitude and frequency values.
The beneficial effects of the utility model：
1st, the utility model band automatically controls the new incubator of oscillating uniform device compared with prior art, and it is shaken up by machinery
Shaken up instead of traditional craft, rapid automatized can realize that the mixing of centrifuge tube inner cell and culture medium in incubator shakes up, no
It address only traditional cell culture incubator and do not possess machine automatization generally and shake up mixed function, be no longer rely on operating personnel and carry out hand
Work is mixed, it is to avoid because the qualification of operating personnel is different and mixing dynamics is difficult to grasp, and causes thin in centrifuge tube
Born of the same parents mix insufficient or cause cell rupture etc., cause to shake up the problem of effect is undesirable.
2nd, the new incubator that band of the present utility model automatically controls oscillating uniform device compared with prior art, utilizes centrifuge
Cell and its cell culture medium are used in conjunction with by principle well with centrifuge tube, it is possible to achieve directly in incubator
Cell centrifugation culture is carried out in centrifuge tube；Such a cell culture mode based on incubator, by centrifuge tube horizontal by 15 DEG C
Inclination angle put, be not only effectively increased surface area of the culture medium with air contact, solve in the prior art because in centrifuge tube train
Foster base table area is too small and influences the shortcoming of centrifuge tube cell culture, also helps and material and energy are carried out between cell and nutrient solution
The abundant exchange of amount, optimizes the effect of cell culture.
3rd, band of the present utility model automatically controls the new incubator of oscillating uniform device, is avoided using mechanical mixture artificial mixed
The problem of efficiency caused is low is closed, while the rotation for being provided with level and vertical direction both direction is mixed, centrifuge tube can be made
Interior cell and the Blending Efficiency of Blending of culture medium reach more preferably.
4th, band of the present utility model automatically controls the new incubator of oscillating uniform device, and the centrifuge tube in incubator can be entered
The rotation of row different directions and concussion is waved by phased manner, it is to avoid rotation for a long time causes to shake up the not good of uniformity, gram
Clothes are easily caused cell in the prior art to be occurred deposition and causes to shake up the problem of effect is not good, it is ensured that the cell in centrifuge tube
Fully mixed with culture medium, be conducive to the culture of lymphocyte to breed, it is possible to resolve conical pipe of the lymphocyte aggregation in centrifuge tube
Bottom is assembled and can not fully contact the defect of culture medium, is greatly enhanced and is shaken up effect.
5th, band of the present utility model automatically controls the new incubator of oscillating uniform device, possesses timing automatic control function, real
Existing cell culture incubator can automatic control type rotating speed, direction of rotation and rotational time, without manually guard on duty always, it is to avoid
Cause the trouble for needing to repeat daily, it is therefore prevented that as operating personnel forget mixing time, mixed sometimes because busy sometimes
The even time just in the morning, night or weekend cause the appearance of the phenomenons such as operating personnel's inconvenience on and off duty.
6th, band of the present utility model automatically controls the new incubator of oscillating uniform device, utilizes the centrifugation being placed in incubator
Pipe carries out cell culture, without cell is transferred in centrifuge tube from blake bottle, simplifies operation, further increases work
Efficiency, saves the quality time of experiment operator, reduces the labor intensity of experiment operator.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model embodiment one；
Fig. 2 is the turntable structure schematic diagram of the utility model embodiment one；
Fig. 3 is the rotating disk sectional view that the utility model applies example one
Fig. 4 is the structural representation of the utility model embodiment two；
Fig. 5 is the structural representation of the utility model embodiment three.
Embodiment
The utility model is further elaborated below in conjunction with the accompanying drawings, wherein, direction of the present utility model is using Fig. 1 as mark
It is accurate.
Embodiment one：
As shown in Figure 1 to Figure 3, band of the present utility model automatically control oscillating uniform device new incubator include casing 1, plus
Hot device 2, hygrosensor 3, oscillating uniform device, centrifuge tube 5 and controller, wherein,
Casing 1 includes tank wall 11, case chamber 12 and chamber door 13, and tank wall 11 is made of heat-insulating heat-preserving material, and tank wall 11 is by case
Chamber 12 is wrapped up inside it, to cause the effect of the holding constant temperature in case chamber 12；Chamber door 13 is arranged on the side wall (casing 1 of tank wall 11
Face side wall just to operating personnel or the side wall of casing 1 for being easy to operating personnel's operation to open) on correspondence breach position, case
Door 13 is double glazing insulated door, and chamber door 13 is hinged on tank wall 11, and the breach of chamber door 13 and tank wall 11 is sealed against, in case
When door 13 is closed, case chamber 12 does not connect to form a confined space with the external world；When chamber door 13 is opened, case chamber 12 passes through tank wall 11
Breach be in communication with the outside.The setting of chamber door 13 so that operating personnel can easily open the chamber door, will be inserted with centrifuge tube 5
Rotating disk 2 insert in case chamber 12.
Heater 2 is used to be internally heated case chamber 12, and heater 2 is arranged in case chamber 12, heater 2 and power supply and
It, which is switched, is electrically connected with.
Hygrosensor 3 is used to the temperature of case chamber 12 is detected and controlled, and hygrosensor 3 is real using prior art
Existing, hygrosensor 3 can also be realized directly using temperature sensor.
Oscillating uniform device is a vertical rotating device 4, and vertical rotating device 4 is used for vertical rotary above and below driving rotating disk progress,
Vertical rotating device 4 includes rotating disk 41, the first rotary shaft 42 and the first motor 43, and rotating disk 41 is used to place centrifuge tube 5 and drive
Centrifuge tube 5 carries out the rotation in vertical direction, and rotating disk 41 is arranged at the middle position of case chamber 12 in axis and plane-parallel, turns
Disk 41 is discoid in thinner thickness, and rotating disk 41 axially and with the axis of rotating disk 41 is provided with some along disk in 15 degree of angles
The circular hole 411 of the centrifuge tube 5 of different capabilities can be planted, some circular holes 411 are evenly arranged on the disk end face of rotating disk 41, this reality
The quantity for applying the circular hole 411 of example is 32, and its particular number can also be set according to actual needs, be not limited in 32；Turn
Position on disk 41 corresponding to each circular hole 411, which is additionally provided with, prevents elastic band or adapter that centrifuge tube 5 comes off to be used to
During preventing that centrifuge tube 5 from rotating with rotating disk 41, shake or come off in circular hole 411；First rotary shaft 42 is in a left side
Right direction is arranged on, its axis and plane-parallel, and the central axis of the first rotary shaft 42 is total to the central axis of rotating disk 41
Line, one end of the first rotary shaft 42 is fixedly connected on the center of rotating disk 41, and the other end of the first rotary shaft 42 passes through tank wall from case
Stretched out in chamber 12, on the output shaft for being connected to the first motor 43 being fixedly installed on outside case chamber 12 so that the first motor 43 drives
Rotating disk 41 carries out the rotation in vertical direction, and the first motor 43 uses stepper motor, and it is electrically connected with the power supply；Wherein, rotating disk
41 and first rotary shaft 42 be Demountable, in order to change the rotating disk 41 of different size and type, to adapt to different fields
The need for conjunction, versatility is stronger.
Centrifuge tube 5 realizes that concrete structure is joined using prior art construction to contain culture medium and culture cell, its structure
See reference Authorization Notice No. CN205182764U utility model patent, in a kind of centrifuge tube knot disclosed in 27 days April in 2016
Structure.
When controller is used for the temperature adjusted in 1 case chamber of control casing 12 and controls rotating speed and the rotation of the first motor 43
Between, electrical equipment, the motor 43 of hygrosensor 3 and first of controller respectively with heater 2 are electrically connected, and control the case of casing 1
The rotation time of temperature, the rotating speed of rotating disk 41 and the first motor 43 in chamber 12.
In use, opening the chamber door 13 of casing 1, the centrifuge tube 5 for being loaded with culture medium and culture cell is planted into cartonning chamber
In the circular hole 411 of 12 inner rotary tables 41, the chamber door 13 of casing 1 is closed, is switched on power, heated by heater 2 to incubator, by temperature
Temperature in the detection case chamber 12 of detector 3, the first motor 43 drives rotating disk 41 to carry out slow uniform rotation on vertical so that turn
The centrifuge tube 5 being obliquely installed on disk 41 will be produced around the axle center of rotating disk 41 in incubator and rotated, the culture medium in centrifuge tube 5
With cell will due to Action of Gravity Field and rotary action continuous transform stream to reaching that cell fully shakes up what is mixed with culture medium
Purpose.By controller according to operating personnel's predetermined temperature value, rotating disk 41 open the period of rotation, rotating disk 41 turn,
Suitable temperature needed for carrying out cell culture to incubator is fine-tuned, and passes through the switch of the first motor of timing controlled 43
Control is timed to the rotation time of rotating disk 41, without personnel it is on duty look after in the case of, realize automatic to rotating disk 41
Rotary state is controlled, and the temperature of incubator is controlled so as to reach, and the timing automatic centrifugation in incubator
Cell and culture medium in pipe 5 carry out fully shaking up mixing.
Embodiment two：
As shown in figure 4, the new incubator that band of the present utility model automatically controls oscillating uniform device includes casing 1, heater
2nd, hygrosensor 3, oscillating uniform device, centrifuge tube 5 and controller, wherein,
Casing 1 includes tank wall 11, case chamber 12 and chamber door 13, and tank wall 11 is made of heat-insulating heat-preserving material, and tank wall 11 is by case
Chamber 12 is wrapped up inside it, to cause the effect of the holding constant temperature in case chamber 12；Chamber door 13 is arranged on the side wall (casing 1 of tank wall 11
Face side wall just to operating personnel or the side wall of casing 1 for being easy to operating personnel's operation to open) on correspondence breach position, case
Door 13 is double glazing insulated door, and chamber door 13 is hinged on tank wall 11, and the breach of chamber door 13 and tank wall 11 is sealed against, in case
When door 13 is closed, case chamber 12 does not connect to form a confined space with the external world；When chamber door 13 is opened, case chamber 12 passes through tank wall 11
Breach be in communication with the outside.The setting of chamber door 13 so that operating personnel can easily open the chamber door, will be inserted with centrifuge tube 5
Rotating disk 2 insert in case chamber 12.
Heater 2 is used to be internally heated case chamber 12, and heater 2 is arranged in case chamber 12, heater 2 and power supply and
It, which is switched, is electrically connected with.
Hygrosensor 3 is used to the temperature of case chamber 12 is detected and controlled, and hygrosensor 3 is real using prior art
Existing, hygrosensor 3 can also be realized directly using temperature sensor.
Oscillating uniform device includes being used for the horizontal rotation apparatus 100 of driving rotating disk progress left and right horizontal rotation and turned for driving
The vertical rotating device 4 of vertical rotary above and below disk progress.
Horizontal rotation apparatus 100 includes base 101, the second rotary shaft 102, electric machine support 103, the second motor 104 and water
Flat rotation platform 105, base 101 is fixedly installed on the bottom of casing 1, and the second rotary shaft 102 is in longitudinally disposed, the second rotary shaft
102 are rotatably provided on base 101, and the top of the second rotary shaft 102 is fixedly connected with the center of horizontal rotatable platform 105, the
The bottom of two rotary shafts 102 is connected with the output shaft of the second motor 104, and the second motor 104 is fixed on bottom by electric machine support 103
The bottom of seat 101, the second motor 104 uses stepper motor, and it is electrically connected with the power supply.
Vertical rotating device 4 includes rotating disk 41, the first rotary shaft 42, the first motor 43 and runing rest 44, runing rest
44 are arranged on the horizontal rotatable platform 105 of horizontal rotation apparatus 100, and runing rest 44 is generally cylindrical, runing rest 44
Axial direction set to left and right direction, the axis of runing rest 44 and horizontal line are set in 15 degree angles, runing rest 44 it is hollow interior
Chamber, which is coated with a housing, housing, is provided with the first motor 43, and the first motor 43 is fixedly mounted on the hollow of runing rest 44
Interior intracavitary, output shaft and the horizontal line of the first motor 43 are rotated in 15 degree of angles (diameter parallel with runing rest 44), first
Axle 42 is rotatably provided on the right-hand member inclined-plane of runing rest 44 in left and right directions, and the first rotary shaft 42 is with horizontal line in 15 degree of angles
(diameter parallel with runing rest 44), the left end of the first rotary shaft 42 is connected with the output shaft of the first motor 43, and first rotates
The right-hand member of axle 42 is extended on the outside of the right-hand member inclined-plane of runing rest 44 and is fixedly attached to rotating disk 41 so that the first motor 43, which drives, to be turned
Disk 41 carries out the rotation in vertical direction, and the first motor 43 uses stepper motor, and it is electrically connected with the power supply；Wherein, rotating disk 41
It is Demountable with the first rotary shaft 42, in order to change the rotating disk 41 of different size and type, to adapt to different occasions
The need for, versatility is stronger.
Rotating disk 41 be used for place centrifuge tube 5 and drive centrifuge tube 5 carry out vertical direction on rotation, rotating disk 41 in axis with
Horizontal line is arranged at the middle position of case chamber 12 in 15 degree of angles (diameter parallel with runing rest 44), and runing rest 44
The line of central axis three of central axis, the central axis of the first rotary shaft 42 and rotating disk 41 is conllinear, and rotating disk 41 is in thinner thickness
Discoid, rotating disk 41 axially and with its diameter parallel is provided with the circle of some centrifuge tubes 5 for planting different capabilities along disk
Hole 411, some circular holes 411 are evenly arranged on the disk end face of rotating disk 41, and the quantity of the circular hole 411 of the present embodiment is 32,
Its particular number can also be set according to actual needs, be not limited in 32；Corresponding to each circular hole 411 on rotating disk 41
Position, which is additionally provided with, prevents elastic band or adapter that centrifuge tube 5 comes off to prevent mistake that centrifuge tube 5 is rotated with rotating disk 41
Cheng Zhong, shakes or comes off in circular hole 411.
Centrifuge tube 5 realizes that concrete structure is joined using prior art construction to contain culture medium and culture cell, its structure
See reference Authorization Notice No. CN205182764U utility model patent, in a kind of centrifuge tube knot disclosed in 27 days April in 2016
Structure.
Controller is used to adjust temperature and the first motor 43 of control and the second motor 104 in 1 case chamber of control casing 12
Rotating speed and rotation time, controller electrical equipment respectively with heater 2, the 43, second electricity of motor of hygrosensor 3 and first
Machine 104 is electrically connected, the rotation time of temperature, the rotating speed of rotating disk 41 and the first motor 43 in 1 case chamber of control casing 12.
In use, opening the chamber door 13 of casing 1, the centrifuge tube 5 for being loaded with culture medium and culture cell is planted into cartonning chamber
In the circular hole 411 of 12 inner rotary tables 41, the chamber door 13 of casing 1 is closed, is switched on power, heated by heater 2 to incubator, by temperature
Temperature in the detection case chamber 12 of detector 3, the first motor 43 drives rotating disk 41 to carry out slow uniform rotation on vertical so that turn
The centrifuge tube 5 being obliquely installed on disk 41 will Vertical Square be rotated up above and below the axle center generation of rotating disk 41 in incubator, together
When the second motor 104 drive horizontal rotatable platform 105 slow uniform rotation is carried out on left and right horizontal direction, installed in level rotation
Turn the rotating disk 41 on platform 105, can also cause the centrifuge tube 5 of rotating disk 41 in incubator around the axle center of horizontal rotatable platform 105
Left and right horizontal side is produced to be rotated up, and because centrifuge tube 5 is placed horizontal by 15 degree of angles, this is allowed for equipped with training
Support the centrifuge tube 5 of base and cell, vertical Action of Gravity Field, horizontally rotate under effect and constantly transform stream to reaching cell and training
Foster base fully shakes up the purpose of mixing.
Rotation is opened according to operating personnel's predetermined temperature value, rotating disk 41 and horizontal rotatable platform 105 by controller
Period, the rotating speed of rotating disk 41, to incubator carry out cell culture needed for suitable temperature fine-tune, and by calmly
When control the switch of the first motor 43 to be timed control to the rotation time of rotating disk 41 and horizontal rotatable platform 105, need not
Personnel are on duty look after in the case of, realize that the automatic rotary state to rotating disk 41, horizontal rotatable platform 105 is controlled so that
Reach and the temperature of incubator is controlled, and the cell and culture medium in the timing automatic centrifuge tube 5 in incubator enter
Row fully shakes up.
Embodiment three：
As shown in figure 5, the new incubator that band of the present utility model automatically controls oscillating uniform device includes casing 1, heater
2nd, hygrosensor 3, oscillating uniform device, centrifuge tube 5 and controller, wherein,
Casing 1 includes tank wall 11, case chamber 12 and chamber door 13, and tank wall 11 is made of heat-insulating heat-preserving material, and tank wall 11 is by case
Chamber 12 is wrapped up inside it, to cause the effect of the holding constant temperature in case chamber 12；Chamber door 13 is arranged on the side wall (casing 1 of tank wall 11
Face side wall just to operating personnel or the side wall of casing 1 for being easy to operating personnel's operation to open) on correspondence breach position, case
Door 13 is double glazing insulated door, and chamber door 13 is hinged on tank wall 11, and the breach of chamber door 13 and tank wall 11 is sealed against, in case
When door 13 is closed, case chamber 12 does not connect to form a confined space with the external world；When chamber door 13 is opened, case chamber 12 passes through tank wall 11
Breach be in communication with the outside.The setting of chamber door 13 so that operating personnel can easily open the chamber door, will be inserted with centrifuge tube 5
Rotating disk 2 insert in case chamber 12.
Heater 2 is used to be internally heated case chamber 12, and heater 2 is arranged in case chamber 12, heater 2 and power supply and
It, which is switched, is electrically connected with.
Hygrosensor 3 is used to the temperature of case chamber 12 is detected and controlled, and hygrosensor 3 is real using prior art
Existing, hygrosensor 3 can also be realized directly using temperature sensor.
Oscillating uniform device includes being used for the horizontal rotation apparatus 100 of driving rotating disk progress left and right horizontal rotation, turned for driving
The rocking equipment 200 that disk progress upset is waved and the vertical rotating device 4 for vertical rotary above and below driving rotating disk progress.
Horizontal rotation apparatus 100 includes base 101, the second rotary shaft 102, electric machine support 103, the second motor 104 and water
Flat rotation platform 105, base 101 is fixedly installed on the bottom of casing 1, and the second rotary shaft 102 is in longitudinally disposed, the second rotary shaft
102 are rotatably provided on base 101, and the top of the second rotary shaft 102 is fixedly connected with the center of horizontal rotatable platform 105, the
The bottom of two rotary shafts 102 is connected with the output shaft of the second motor 104, and the second motor 104 is fixed on bottom by electric machine support 103
The bottom of seat 101, the second motor 104 uses stepper motor, and it is electrically connected with the power supply.
Rocking equipment 200 includes swaying base 201, the 3rd motor 202, eccentric wheel 203 and Swing Arm 204, swaying base
201 are fixedly mounted on the horizontal rotatable platform 105 of horizontal rotation apparatus 100, and swaying base 201 is tower structure, the 3rd
Motor 202 is fixedly mounted on the lower portion of swaying base 201, and the 3rd motor 202 uses stepper motor, and it electrically connects with power supply
Connect, eccentric wheel 203 is arranged on the power output shaft of the 3rd motor 202, and one end of Swing Arm 204 is hinged on eccentric wheel 203,
A pair of circular pins are additionally provided with the position of the right edge of swaying base 201.
Vertical rotating device 4 includes rotating disk 41, the first rotary shaft 42, the first motor 43 and runing rest 44, runing rest
44 is generally cylindrical, and the axial direction of runing rest 44 is set to left and right direction, and the left side middle position of runing rest 44 is with waving
The other end of arm 204 is hinged, and the lower position in the left side of runing rest 44 passes through the right edge of circular pin and swaying base 201
It is hinged along position, and corresponding to the breach that camber is set in the hunting range of runing rest 44 on swaying base 201, so that
Obtain runing rest 44 has a space mutually avoided during swinging, it is to avoid occur swaying base 201 and rotation
Support 44 collides.
The axis of runing rest 44 is set with horizontal line in 15 degree of angles, and the hollow cavity of runing rest 44 is coated with a shell
The first motor 43 is provided with body, housing, the first motor 43 is fixedly mounted in the hollow cavity of runing rest 44, the first motor
43 output shaft is with horizontal line in 15 degree of angles (diameter parallel with runing rest 44), and the first rotary shaft 42 can in left and right directions
It is rotatably arranged on the right-hand member inclined-plane of runing rest 44, the first rotary shaft 42 is with horizontal line in 15 degree of angles (with runing rest 44
Diameter parallel), the left end of the first rotary shaft 42 is connected with the output shaft of the first motor 43, and the right-hand member of the first rotary shaft 42 is extended to
Runing rest 44 is fixedly attached to rotating disk 41 on the outside of right-hand member inclined-plane so that the first motor 43 drives rotating disk 41 to carry out vertical direction
On rotation, the first motor 43 uses stepper motor, and it is electrically connected with the power supply；Wherein, rotating disk 41 is with the first rotary shaft 42
Demountable, in order to change the rotating disk 41 of different size and type, the need for adapting to different occasions, versatility is more
By force.
Rotating disk 41 be used for place centrifuge tube 5 and drive centrifuge tube 5 carry out vertical direction on rotation, rotating disk 41 in axis with
Horizontal line is arranged at the middle position of case chamber 12 in 15 degree of angles (diameter parallel with runing rest 44), and runing rest 44
The line of central axis three of central axis, the central axis of the first rotary shaft 42 and rotating disk 41 is conllinear, and rotating disk 41 is in thinner thickness
Discoid, rotating disk 41 axially and with its diameter parallel is provided with the circle of some centrifuge tubes 5 for planting different capabilities along disk
Hole 411, some circular holes 411 are evenly arranged on the disk end face of rotating disk 41, and the quantity of the circular hole 411 of the present embodiment is 32,
Its particular number can also be set according to actual needs, be not limited in 32；Corresponding to each circular hole 411 on rotating disk 41
Position, which is additionally provided with, prevents elastic band or adapter that centrifuge tube 5 comes off to prevent mistake that centrifuge tube 5 is rotated with rotating disk 41
Cheng Zhong, shakes or comes off in circular hole 411.
Centrifuge tube 5 realizes that concrete structure is joined using prior art construction to contain culture medium and culture cell, its structure
See reference Authorization Notice No. CN205182764U utility model patent, in a kind of centrifuge tube knot disclosed in 27 days April in 2016
Structure.
The first motor 43 of temperature and control that controller is used to adjust in control casing 1 case chamber 12, the second motor 104 with
And the 3rd motor 202 rotating speed and rotation time, electrical equipment, the hygrosensor 3 and first of controller respectively with heater 2
Motor 43, the second motor 104 and the 3rd motor 202 are electrically connected, temperature, the rotating speed of rotating disk 41 in 1 case chamber of control casing 12
And first motor 43 rotation time.
In use, opening the chamber door 13 of casing 1, the centrifuge tube 5 for being loaded with culture medium and culture cell is planted into cartonning chamber
In the circular hole 411 of 12 inner rotary tables 41, the chamber door 13 of casing 1 is closed, is switched on power, heated by heater 2 to incubator, by temperature
Temperature in the detection case chamber 12 of detector 3, the first motor 43 drives rotating disk 41 to carry out slow uniform rotation on vertical so that turn
The centrifuge tube 5 being obliquely installed on disk 41 will in incubator around rotating disk 41 axle center produce above and below Vertical Square rotate up turn
It is dynamic, while the second motor 104 drives horizontal rotatable platform 105 to carry out slow uniform rotation on left and right horizontal direction, it is arranged on
Rotating disk 41 on horizontal rotatable platform 105, can also cause the centrifuge tube 5 of rotating disk 41 in incubator around horizontal rotatable platform 105
Axle center produce rotary motion on left and right horizontal direction, and because centrifuge tube 5 is that this is just horizontal by 15 degree angles placements
So that culture medium and cell in centrifuge tube 5, vertical Action of Gravity Field, horizontally rotate under effect and constantly transform stream to reaching
Cell fully shakes up the purpose mixed with culture medium.
Meanwhile, the first motor 43, the rotating speed of the second motor 104 and different switches that controller 6 is set according to operating personnel
State, so that it may which realization is placed with the rotating disk 41 of centrifuge tube 5 and rotated with different rotating speeds, towards different directions；In addition, in order to
Overcome and be easily caused cell generation deposition in the prior art and cause to shake up the problem of effect is not good, it is ensured that be in centrifuge tube thin
Born of the same parents fully mix with culture medium, are conducive to the culture of lymphocyte to breed, it is possible to resolve taper of the lymphocyte aggregation in centrifuge tube
Ttom of pipe assemble and can not fully contact the defect of culture medium, the present embodiment can also by controller it is presetting when, intermittently starting
3rd motor 202 of rocking equipment 200 is driven with movable eccentric wheel 203 and Swing Arm 204 by eccentric wheel 203 and Swing Arm 204
Runing rest 44 vacillate now to the left, now to the right rocking, and realizes the centrifuge tube 5 on rotating disk 41 in the same of vertical up and down and left and right horizontal rotation
When produce periodically intermittently wave concussion, concussion can be waved by phased manner to the centrifuge tube 5 in incubator, it is to avoid
Rotation causes to shake up the not good of uniformity for a long time, is greatly enhanced and shakes up effect.In the course of work of rocking equipment 200, and
It can be selected according to three kinds of different concussion patterns of waving：Under one is inactive state, the centrifuge tube on rotating disk 41 is kept to be in
It is in the position at 15 degree of inclinations angle with horizontal line, and faces operating personnel, so that operating personnel enters to the centrifuge tube 5 on rotating disk 41
Row is inserted and taken out；Two be wave concussion during have an extreme position, the centrifuge tube 5 in this position, circular hole 411 can be located
In horizontality；Three be wave concussion realization shake up during, the amplitude-controllable system waved is between 45 degree to 90 degree, OK
Process control is within 20 centimetres, and FREQUENCY CONTROL waves concussion control in the scope of 5-10 times per second successively every time.
Rotation is opened according to operating personnel's predetermined temperature value, rotating disk 41 and horizontal rotatable platform 105 by controller
Period, rotating disk 41 turn, to incubator carry out cell culture needed for suitable temperature fine-tune, and pass through regularly
The switch of the first motor 43 is controlled to be timed control to the rotation time of rotating disk 41 and horizontal rotatable platform 105, without people
Member is on duty look after in the case of, realize that the automatic rotary state to rotating disk 41, horizontal rotatable platform 105 is controlled, so as to reach
It is controlled to the temperature to incubator, and the cell and culture medium in the timing automatic centrifuge tube 5 in incubator are carried out
Fully shake up.
The present embodiment is additionally provided with the input/output module being connected with controller, display module and timer (in figure not
Show), the temperature value, disc rotation speed, disk that operating personnel can input setting in input/output module turn to, wave concussion
Amplitude and frequency values, and can show that the temperature value, disc rotation speed, disk of work at present turn to, wave shake in real time by display module
Amplitude and frequency values, and the first motor 43, the second motor 104, the 3rd motor 202 are swung in the preset time of subscription time section
Value, the first motor 43, the second motor 104, can be timed open and close in the section at any time of the 3rd motor 202,
The first motor 43, the second motor 104, the 3rd motor 202 can be set or work independently or while work or group two-by-two
Worked while conjunction form, i.e., specific running parameter can carry out any setting with mode of operation according to actual conditions.
The better embodiment of the present invention is the foregoing is only, the invention is not limited in above-mentioned embodiment, is being implemented
During there may be local small structural modification, if the various changes or modification to the present invention do not depart from the essence of the present invention
God and scope, and belong within the scope of the claim and equivalent technologies of the present invention, then the present invention is also intended to comprising these changes
And modification.
Claims (10)
1. a kind of band automatically controls the new incubator of oscillating uniform device, it includes that heat-insulated casing can be closed, by heater, temperature
The temperature control system of detector and controller composition, the casing is provided with case chamber, the heater and the temperature sensing
Device is arranged at case intracavitary, and the controller is arranged on casing, it is characterised in that：It also includes oscillating uniform device and timer,
The oscillating uniform device includes horizontal rotation apparatus and vertical rotating device, and the horizontal rotation apparatus includes base, the second rotation
Axle, the second motor and horizontal rotatable platform, the base are arranged on the bottom of the casing case chamber, and second rotary shaft can turn
Dynamic to be arranged on base, the case intracavitary of the casing is stretched into described second rotary shaft one end, and is connected to and is arranged at case intracavitary
Horizontal rotatable platform, the other end of second rotary shaft is rushed across base and is connected with the output shaft of second motor, and described
Two motors are fixedly mounted on the bottom of the base by the electric machine support；
The vertical rotating device is arranged on the case intracavitary, and the vertical rotating device includes rotating disk, the first rotary shaft, first
Motor and runing rest, the runing rest are arranged on the horizontal rotatable platform, and first rotary shaft is rotatably set
On the runing rest, first motor is fixedly installed in the hollow cavity of the runing rest, first motor
Output shaft be connected with one end of first rotary shaft, the other end of first rotary shaft is fixedly connected with the rotating disk,
The circular hole of some centrifuge tubes for inserting various different capabilities is provided with the rotating disk；
The timer is used to be timed control, the timing to the working time of first motor and second motor
Device and first motor are electrically connected with second motor, the controller and heater, hygrosensor, timer,
First motor and the second motor are electrically connected with, with determining for the temperature in control cabinet, the rotating speed of rotating disk, the steering of rotating disk and rotating disk
When mode of operation.
2. band according to claim 1 automatically controls the new incubator of oscillating uniform device, it is characterised in that：It is described to shake up dress
Putting also includes rocking equipment, and the rocking equipment includes swaying base, the 3rd motor, eccentric wheel and Swing Arm, described to wave base
Seat is arranged on the horizontal rotatable platform, and the 3rd motor is fixedly mounted on the lower portion of the swaying base, described
Eccentric wheel is arranged on the power output shaft of the 3rd motor, and one end of the Swing Arm is hinged on eccentric wheel, described to shake
The other end of swing arm is hinged on the runing rest, and the edge portion of the swaying base is provided with a pair of circular pins, described
Swaying base is hinged by a pair of circular pins with the runing rest.
3. band according to claim 2 automatically controls the new incubator of oscillating uniform device, it is characterised in that：Waved described
Corresponding to the breach that camber is set in the hunting range of the runing rest on pedestal, to cause the runing rest reciprocal
There is a space mutually avoided during swing, it is to avoid swaying base collides with runing rest.
4. band according to claim 1 automatically controls the new incubator of oscillating uniform device, it is characterised in that：The rotation branch
Frame is generally cylindrical, and the runing rest axis is set with horizontal line in 15 degree of angles.
5. band according to claim 1 automatically controls the new incubator of oscillating uniform device, it is characterised in that：The rotating disk is in
Axis and plane-parallel, the rotating disk are arranged at the case intracavitary middle position of the casing.
6. band according to claim 1 automatically controls the new incubator of oscillating uniform device, it is characterised in that：On the rotating disk
The circular hole axially penetrated through along it is provided with, the quantity of the circular hole is at least one.
7. band according to claim 6 automatically controls the new incubator of oscillating uniform device, it is characterised in that：The rotating disk is in
Thinner thickness it is discoid, the circular hole is evenly arranged on the disk end face of the rotating disk.
8. band according to claim 6 automatically controls the new incubator of oscillating uniform device, it is characterised in that：The circular hole
Central axis intersects with the central axis of the rotating disk in 15 degree of angles.
9. band according to claim 6 automatically controls the new incubator of oscillating uniform device, it is characterised in that：On the rotating disk
The elastic band or adapter that prevent centrifuge tube from coming off is additionally provided with corresponding to the position of each circular hole, to prevent centrifuge tube
Shake or come off out of circular hole when being rotated with rotating disk.
10. band according to claim 1 automatically controls the new incubator of oscillating uniform device, it is characterised in that：The casing
On be provided with the chamber door for being easy to operating personnel the rotating disk for being inserted with centrifuge tube to be inserted or taken out case chamber, the casing and also set up
There are the input/output module being electrically connected with controller, display module, so that operating personnel can input in input/output module
The temperature value of setting, disc rotation speed, disk turn to, wave shock range and frequency values, and can in real time be shown and work as by display module
Temperature value, disc rotation speed, the disk of preceding work turn to, wave shock range and frequency values.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720041820.4U CN206396247U (en) | 2017-01-14 | 2017-01-14 | Band automatically controls the new incubator of oscillating uniform device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720041820.4U CN206396247U (en) | 2017-01-14 | 2017-01-14 | Band automatically controls the new incubator of oscillating uniform device |
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| Publication Number | Publication Date |
|---|---|
| CN206396247U true CN206396247U (en) | 2017-08-11 |
Family
ID=59513724
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720041820.4U Active CN206396247U (en) | 2017-01-14 | 2017-01-14 | Band automatically controls the new incubator of oscillating uniform device |
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| Country | Link |
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| CN (1) | CN206396247U (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603879A (en) * | 2017-10-31 | 2018-01-19 | 广州和能生物科技有限公司 | A kind of band automatically controls the new incubator of oscillating uniform device |
| CN108014706A (en) * | 2018-01-09 | 2018-05-11 | 钦州学院 | Suitable for the intelligent oscillating uniform device of laboratory reagent |
| CN108142214A (en) * | 2018-01-03 | 2018-06-12 | 河南天蚕生物科技有限公司 | A kind of vertical Cordyceps Militaris spreads cultivation device and its bottle that spreads cultivation |
| CN108770108A (en) * | 2018-08-31 | 2018-11-06 | 四川国星高分子树脂有限公司 | A kind of pitch softening microwave heating box |
| CN109497040A (en) * | 2018-11-16 | 2019-03-22 | 浙江大学 | A kind of human oocytes and embryo vitrifying freeze thawing device and its application method |
| CN110540927A (en) * | 2018-05-28 | 2019-12-06 | 中国科学院城市环境研究所 | High-throughput sample rotary incubator |
| CN110819534A (en) * | 2019-11-27 | 2020-02-21 | 西北师范大学 | A automatic cell culture device for mouse uterus matrix |
| CN111172032A (en) * | 2020-02-19 | 2020-05-19 | 海口健康岛生物科技有限公司 | Automatic incubator for living cells |
| CN112457979A (en) * | 2020-11-19 | 2021-03-09 | 江西迈柯菲生物医药科技有限公司 | Double-cylinder centrifugal large-scale suspension type cell culture device |
| CN113106061A (en) * | 2021-05-07 | 2021-07-13 | 恩大细胞基因工程有限公司 | Method for long-term storage and recovery culture of single cells |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603879A (en) * | 2017-10-31 | 2018-01-19 | 广州和能生物科技有限公司 | A kind of band automatically controls the new incubator of oscillating uniform device |
| CN107603879B (en) * | 2017-10-31 | 2019-01-15 | 广州和能生物科技有限公司 | A kind of band automatically controls the incubator of oscillating uniform device |
| CN108142214A (en) * | 2018-01-03 | 2018-06-12 | 河南天蚕生物科技有限公司 | A kind of vertical Cordyceps Militaris spreads cultivation device and its bottle that spreads cultivation |
| CN108014706A (en) * | 2018-01-09 | 2018-05-11 | 钦州学院 | Suitable for the intelligent oscillating uniform device of laboratory reagent |
| CN110540927A (en) * | 2018-05-28 | 2019-12-06 | 中国科学院城市环境研究所 | High-throughput sample rotary incubator |
| CN108770108A (en) * | 2018-08-31 | 2018-11-06 | 四川国星高分子树脂有限公司 | A kind of pitch softening microwave heating box |
| CN109497040A (en) * | 2018-11-16 | 2019-03-22 | 浙江大学 | A kind of human oocytes and embryo vitrifying freeze thawing device and its application method |
| CN110819534A (en) * | 2019-11-27 | 2020-02-21 | 西北师范大学 | A automatic cell culture device for mouse uterus matrix |
| CN111172032A (en) * | 2020-02-19 | 2020-05-19 | 海口健康岛生物科技有限公司 | Automatic incubator for living cells |
| CN112457979A (en) * | 2020-11-19 | 2021-03-09 | 江西迈柯菲生物医药科技有限公司 | Double-cylinder centrifugal large-scale suspension type cell culture device |
| CN113106061A (en) * | 2021-05-07 | 2021-07-13 | 恩大细胞基因工程有限公司 | Method for long-term storage and recovery culture of single cells |
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Address after: 510000 A unit 17, No. 17, Zhenxing street, Haizhuqu District, Guangzhou, Guangdong. Patentee after: Guangzhou and energy bio tech ltd Address before: 510000 self made 108B room in courtyard 189, don and road, Haizhuqu District, Guangzhou, Guangdong, China. Patentee before: Guangzhou and energy bio tech ltd |