CN111171999A - Ant health care wine and preparation method thereof - Google Patents
Ant health care wine and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of ant wine, and relates to ant health care wine and a preparation method thereof, wherein the ant health care wine comprises the following substances in parts by weight mixed with effective components in white spirit: 40-70 parts of polyrhachis vicina Roger, 10-40 parts of American ginseng, 30-60 parts of rhodiola rosea, 5-35 parts of kudzu root and 10-30 parts of wolfberry fruit. The ant wine has the advantages of precise and appropriate formula compatibility, short time consumption, high extraction efficiency, obvious effect and good taste.
Description
Technical Field
The invention belongs to the field of ant wine, and relates to ant health-care wine and a preparation method thereof.
Background
The combination of wine and medicine is an important pioneer in the medical development history of China. Modern medicine proves that when drinking properly, the wine can dredge collaterals, expel wind, relax muscles and tendons, promote blood circulation, dispel cold, warm the body, eliminate food retention, invigorate the spleen, calm the nerves and calm the mind, and has certain effects of treating diseases and protecting health.
China is the origin of ants for edible and medicinal purposes, and is recorded in compendium of materia Medica: "Yili is the biggest, can act as an equivalent body iron, and can benefit strength and luster when eaten by people. The polyrhachis vicina Roger with high nutritive value, especially the only medicine and food dual-purpose value approved by the Ministry of health, contains more than 70 nutritive components, 42-67% of protein and 28 free amino acids; vitamin B1, B2, B12, C, D, E and the like; more than 20 trace elements such as calcium, iron, phosphorus, manganese, selenium, zinc and the like are contained, particularly, the zinc content which is known as 'life spark' is most abundant, and each kilogram of zinc contains 120-198 mg; contains various bioactive substances such as triterpenes, formic acid, Adenosine Triphosphate (ATP), artificial nonsynthesized praecox forminal, interleukin-2 and the like, and the content of the bioactive substances is named as imperata in millions of animals and plants; ant has wide pharmacological action, and has effects in improving immunity, endurance, antiaging, antiinflammatory, analgesic, hepatoprotective, antiasthmatic, and tranquilizing; has prominent clinical advantages, and is often used as an immunity enhancer, an enhancer for improving the endurance of the organism, a sexual function enhancer and the like in clinic.
The ant-soaked wine is inherited from ancient times, more and more ant health-care wine is developed in recent years, but the wine is often processed roughly, takes long time, has a single prescription, has unobvious effect or has poor taste.
Disclosure of Invention
The ant health wine has the advantages of precise and appropriate compatibility, short time consumption, high extraction efficiency, obvious effect and good taste.
In order to achieve the technical purpose, the technical scheme adopted by the application is that the ant health-care wine comprises the following substances in parts by weight of effective components mixed in white spirit:
as an improved technical scheme of the application, the wine comprises the following substances of effective components mixed in the white wine in parts by weight:
as an improved technical scheme of the application, the white spirit is made of grains as raw materials, and the alcoholic strength of the white spirit is 50-60% v/v.
As the improved technical scheme of the application, the using amount of the white spirit is 6-10 times of the total feeding amount of all the substances.
Another objective of the present application is to provide a method for preparing ant health wine, comprising:
grinding, namely grinding the components in proportion into powder;
soaking, namely soaking the ground material into white spirit to obtain a suspension;
filtering, namely filtering the soaked suspension liquid to obtain a liquid component;
diluting, diluting the filtered liquid component with purified water to alcohol content of 38%;
and (4) packaging, namely canning and packaging the diluted liquid.
As an improved technical scheme of the application, the soaking temperature is between 12 and 30 ℃, and the soaking time is between 20 and 35 days.
As an improved technical scheme of the application, the components with the proportion are ground into powder, and the mesh number of the powder is between 20 and 80 meshes.
Another objective of the present application is to provide a method for preparing ant health wine, comprising:
proportioning, and obtaining the proportioned and crushed substances;
extracting effective components, namely extracting the effective components of each substance in a proportioning amount by taking white spirit as a solvent;
filtering, and filtering the extracted liquid to obtain a filtrate;
diluting the filtrate to 38% alcohol content;
canning and packaging.
As an improved technical scheme of the application, the extraction of the effective components is reflux extraction of the effective components by adopting white spirit as a solvent.
As an improved technical scheme of the application, the alcoholic strength of the white spirit is between 50% and 60% v/v.
Advantageous effects
The ant health-care wine has the advantages of strong pertinence of raw materials and precise and appropriate compatibility: the prescription takes polyrhachis vicina Roger as monarch drug, American ginseng and rhodiola rosea as ministers, radix puerariae as assistants and medlar as guides, and has the effects of benefiting qi and nourishing blood, nourishing yin and yang, and tonifying qi and blood and regulating yin and yang. Can enhance immunity and relieve physical fatigue.
The polyrhachis vicina Roger is a monarch drug, has salty and mild taste, enters liver and kidney channels, and has the effects of nourishing liver and kidney, strengthening tendons and bones, dispelling wind and dredging collaterals.
American ginseng, sweet, slightly bitter and cool. It enters heart, lung and kidney meridians. Has the effects of invigorating qi, nourishing yin, clearing heat and promoting fluid production; rhodiola root is sweet, bitter and neutral. It enters lung and heart meridians. Has effects of invigorating qi, promoting blood circulation, dredging collaterals, and relieving asthma.
Kudzuvine root is sweet, pungent and cool. It enters spleen, stomach and lung meridians. Has the effects of expelling pathogenic factors from muscles, relieving fever, promoting salivation, quenching thirst, promoting eruption, invigorating yang, relieving diarrhea, dredging meridian passage, and relieving alcoholism.
Wolfberry fruit is sweet and neutral in taste. It enters liver and kidney meridians. Has the effects of nourishing liver and kidney, replenishing vital essence and improving eyesight.
This application is in the preparation process, and extraction efficiency is high: the raw materials are crushed to accelerate the dissolution of the medicine; meanwhile, the preparation time is shortened by heating and refluxing, and the extraction efficiency is high.
Sterile pollution and safe use: the wine-making source medicinal material powder is prepared after sterilization, the finished product also needs sterilization before subpackaging, and the medicinal materials of the prescription of the invention, namely polyrhachis vicina roger, American ginseng, rhodiola rosea, radix puerariae and medlar are medicinal and edible medicinal materials, so that the safety coefficient is higher.
Comfortable taste and high nutritive value: the American ginseng, the kudzuvine root and the medlar are mixed in the prescription, so that the nutritional value of the medicinal liquor is enhanced, and the medicinal taste is alleviated, and the taste of the medicinal liquor is comfortable and soft. The polyrhachis vicina Roger is used as monarch drug, has large prescription amount and rich nutritional value, contains more than 70 nutritional ingredients in vivo, and can be called as 'micro nutritional treasury'.
In addition, in the preparation process, the medicinal materials of the health-care wine formula are crushed, the contact between the medicinal materials and the wine is increased, more medicinal components are promoted to be dissolved out, meanwhile, the medicine dissolution is accelerated and the wine making time is shortened by adopting a heating reflux extraction mode, and the comparison and research of pharmacodynamic experiments show that the health-care wine formula has high production efficiency, stable product quality and obvious curative effect and is suitable for large-scale production.
Detailed Description
In order to make the purpose and technical solution of the embodiments of the present invention clearer, the technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the invention without any inventive step, are within the scope of protection of the invention.
It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The ant health wine comprises the following effective components in parts by weight dissolved in white spirit:
40-70 parts by weight of polyrhachis vicina roger
10-40 parts of American ginseng
30-60 parts of rhodiola rosea
5-35 parts by weight of kudzu root
10-30 parts of wolfberry fruit.
The above formula most preferably comprises the following substances in parts by weight:
60 parts of polyrhachis vicina Roger, 20 parts of American ginseng, 40 parts of rhodiola rosea, 20 parts of kudzu root and 20 parts of wolfberry fruit.
(2) Weighing corresponding medicinal materials according to the formula proportion, crushing the medicinal materials in the formula to 65 meshes (65 meshes are the optimal value, and can be selected to be 20-80 meshes, in order to ensure the efficiency maximization, the 65 meshes are selected in the embodiment), and sterilizing;
(3) the first preparation method comprises the following steps: packaging the medicinal powder into a bag, adding Chinese liquor (with alcohol content of 50-60% v/v, preferably 55% v/v) prepared from grain as raw material into a soaking container, soaking the medicinal materials at 12-30 deg.C for 20-35 days, filtering, diluting the filtrate with purified water to alcohol content of 38%, bottling, packaging in sterilized wine bottle, sealing, and packaging.
(4) The second preparation method comprises the following steps: weighing the corresponding medicinal materials according to the above formula proportion, reflux-extracting Chinese liquor (with alcohol content of 50-60% v/v, preferably 55% v/v) prepared from grain as raw material with solvent, filtering, diluting the filtrate with purified water to alcohol content of 38%, feeding into a canning machine, filling into sterilized wine bottle, sealing, and packaging.
Example 1
40g of polyrhachis vicina Roger, 10g of American ginseng, 30g of rhodiola rosea, 5g of radix puerariae and 10g of wolfberry fruit are crushed, sieved by 65 meshes, put into a medicine bag and a traditional Chinese medicine dipping container, then 8 times of white spirit (the alcoholic strength is 55% v/v) prepared by taking grains as raw materials is poured into the dipping container, the medicinal materials are dipped and soaked for 20 days at the temperature of 12-30 ℃, filtered, the filtrate is diluted by purified water until the alcoholic content is 38%, the filtrate is sent into a canning machine, and the sterilized wine bottle is filled with the filtrate for sealing and packaging, thus obtaining the medicine.
Example 2
40g of polyrhachis vicina Roger, 10g of American ginseng, 30g of rhodiola rosea, 5g of radix puerariae and 10g of wolfberry fruit are crushed, sieved by a 65-mesh sieve, and subjected to reflux extraction by using 8 times of white spirit (the alcoholic strength is 55% v/v) prepared by taking grains as a raw material as a solvent, the filtration is performed, the filtrate is diluted by purified water to the alcoholic content of 38%, the filtrate is sent into a canning machine, and the sterilized wine bottle is filled with the filtrate, and then the wine is sealed and packaged, so that the polyrhachis.
Example 3
60g of polyrhachis vicina Roger, 20g of American ginseng, 40g of rhodiola rosea, 20g of radix puerariae and 20g of wolfberry fruit are crushed, sieved by 65 meshes, put into a medicine bag and a traditional Chinese medicine dipping container, 10 times of white spirit (the alcoholic strength is 50% v/v) prepared by taking grains as raw materials is poured into the dipping container to dip the medicinal materials, the medicinal materials are soaked for 20 days at the temperature of 12-30 ℃, filtered, the filtrate is diluted by purified water until the alcoholic content is 38%, the filtrate is sent into a canning machine, and the sterilized wine bottle is filled with the filtrate to be sealed and packaged, thus obtaining the medicine.
Example 4
60g of polyrhachis vicina roger, 20g of American ginseng, 40g of rhodiola rosea, 20g of radix puerariae and 20g of wolfberry fruit are crushed, sieved by a 65-mesh sieve, extracted by refluxing with 10 times of white spirit (the alcoholic strength is 50% v/v) prepared by taking grains as a solvent, filtered, diluted by purified water to reach the alcoholic content of 38%, sent into a canning machine, filled into a sterilized wine bottle, and sealed and packaged to obtain the polyrhachis vicina roger wine.
Example 5
70g of polyrhachis vicina Roger, 40g of American ginseng, 60g of rhodiola rosea, 35g of radix puerariae and 30g of wolfberry fruit are crushed, sieved by 65 meshes, put into a medicine bag and a traditional Chinese medicine dipping container, then 6 times of white spirit (the alcoholic strength is 60% v/v) prepared by taking grains as raw materials is poured into the dipping container, the medicinal materials are dipped and soaked for 20 days at the temperature of 12-30 ℃, filtered, the filtrate is diluted by purified water until the alcoholic content is 38%, the filtrate is sent into a canning machine, and the sterilized wine bottle is filled with the filtrate for sealing and packaging, thus obtaining the medicine.
Example 6
70g of polyrhachis vicina Roger, 40g of American ginseng, 60g of rhodiola rosea, 35g of radix puerariae and 30g of wolfberry fruit are crushed, sieved by a 65-mesh sieve, extracted by refluxing by using 6 times of white spirit (the alcoholic strength is 60% v/v) prepared by taking grains as a solvent, filtered, diluted by purified water to reach the alcoholic content of 38%, sent into a canning machine, filled into a sterilized wine bottle, and sealed and packaged to obtain the polyrhachis vicina Roger tea.
a, taking mouse swimming under load, serum urea, liver glycogen content and the like as indexes, investigating the anti-fatigue effect of the ant health-care wine, and carrying out comparative study with the commercially available black ant health-care wine (Guangxi Liuzhou Longwan distillery) and Shenli ant wine (Nenggang Shenlong health product Co., Ltd.):
ant health wine, example 1, example 2, example 3, example 4, example 5, example 6, black ant health wine, chard ant wine, 8 experimental groups, wine-based control group and distilled water control group, each group containing 10 male mice. Each group of animals is administered with corresponding medicine 0.2ml/10g by intragastric administration every day, and the administration is performed for 1 time every day, and after 30 consecutive days, each anti-fatigue index is analyzed and determined.
1. Mouse weight-bearing swimming test the test object was given 30min at the last time, weighed, and each mouse tail was loaded with 5% weight lead skin, and placed in a swimming box for swimming, and the time from the start of swimming to death was recorded (water depth (33 shi 1) cm, water temperature (25 shi 1) ° c at room temperature and time with stirring, and the four limbs of the mouse were kept in motion).
2. Liver glycogen determination (anthrone method) the pre-test substance was administered 30min at the last time, weighed, sacrificed, the liver was taken, washed, blotted dry, 200mg was weighed accurately, 4.0mL of trichloroacetic acid was added, homogenized for 1min, centrifuged at 3000r/min for 15min, the supernatant was transferred to another tube, 4.0mL of trichloroacetic acid was added to the precipitate, homogenized for 1min, and centrifuged again for 15 min. Mixing the two supernatants, placing 1.0mL into a 10mL centrifuge tube, adding 4.0mL of 95% ethanol, mixing, plugging, placing in water bath at 39 st 1 deg.C for 3h, precipitating completely, centrifuging at 3000r/min for 15min, discarding supernatant, and standing upside down for 10 min. Dissolving hepatic glycogen in 2.0mL of distilled water, adding 2.0mL of distilled water into a blank tube, adding 0.5mL of glucose standard solution and 1.5mL of distilled water into a standard tube, adding 10.0mL of anthrone reagent into each tube, immediately placing a test tube into a cold water bath to reach the water temperature, placing the test tube into a boiling water bath for 15min, moving the test tube into the cold water bath to cool to the room temperature, carrying out color comparison at the wavelength of 620nm, carrying out zero setting by using the blank tube, and calculating the hepatic glycogen content.
3. Blood lactic acid, serum urea nitrogen, serum lactic acid denitrase and blood sugar are measured, the pre-test object is given for 30min at the last time, the weight is weighed, 2% weight lead skin is loaded on the tail part of each mouse, the mouse is placed in a swimming box for swimming (the operation is the same as above), the mouse is taken out at the 60 th min, the mouse is kept still for 15min, and the eyeball is picked for blood collection. Preparing serum, and measuring urea nitrogen, lactate dehydrogenase and blood sugar in the serum by using a full-automatic biochemical analyzer. Adding 0.48mL of 1% NaF solution into a 5mL test tube, accurately sucking 20mL of whole blood, adding the whole blood into the bottom of the test tube, washing the micropipette with supernatant in the test tube for times, adding 1.5mL of protein precipitant, and shaking uniformly. Centrifuging at 3000r/min for 10And (5) min. Adding supernatant 0.5mL into precipitation tube, adding lactic acid standard application solution 0.5mL into standard tube, adding precipitant-NaF mixed solution 0.5mL into blank tube, adding 0.1mL 4% CUS0 into each tube4Then adding 3.0mL of concentrated sulfuric acid, fully mixing, heating in a boiling water bath for 5min, taking out, putting in an ice water bath, cooling for 10min, adding 0.1mL of 0.5% p-biphenylene into each tube, shaking up, putting in a (30 Shi 1) DEG C water bath for 30min, putting in a boiling water bath, heating for 1.5min, taking out, cooling to room temperature, carrying out color comparison at a wavelength of 560nm by using a 5mm cuvette, adjusting the blank tube to zero, and calculating the content of the blood lactic acid.
Table 1 results of fatigue resistance test (n ═ 10, X shi S)
P <0.05 compared to distilled water control; p <0.01 compared to distilled water control.
Examples 1 and 2 can reduce the content of urea nitrogen in mouse serum, reduce the content of lactate dehydrogenase in mouse serum, increase the blood sugar of mouse, and prolong the swimming time, and examples 3 and 4 have inferior effects to the ant wine and the black ant health wine. The ant health care wine has the function of fatigue resistance.
b, examining the influence of the ant health-care wine on immune organs, phagocytic indexes and delayed allergy of mice by a model of low immunity caused by hydrocortisone.
NIH mice, male and female halves, were randomly divided into 10 groups, each group containing 10 animals, namely a normal group, a model group, an ant health wine group of example 1, an example 2, an example 3, an example 4, an example 5, an example 6, a black ant health wine group, and a Shenli ant wine group. Each group of animals was gavaged daily with the corresponding drug 0.2ml/10g, and the normal group was given the same volume of distilled water 1 time/day for 8 days. Meanwhile, except for the normal control group, all the animals of the other groups are simultaneously injected with hydrocortisone 40mg/kg subcutaneously to cause a low immunity model for 1 time/day and 8 days continuously.
Immune organ effects: the animals are dislocated and sacrificed after the last administration for 1h, the weights of the spleen and the thymus are weighed, the organ indexes are calculated, and the differences of all groups are compared.
Influence of non-specific immune function: 1h after the last administration, 5ml/kg body weight of India ink is injected into tail vein, 20ul blood is taken from mouse fundus venous plexus at 2min and 8min after injection, and 0.1% sodium bicarbonate solution 2ml is added and shaken up. The Optical Density (OD) at 680nm was measured by 722 spectrophotometry and the OD values were measured as OD2And OD8the optical density of the 2min and 8min samples was expressed, and the blank mice were zeroed with a mixture of blood and 0.1% sodium bicarbonate solution, and finally the mice were sacrificed, and the spleen and liver were weighed, and the clearance index K and the phagocytosis index α values were calculated according to the following formulas.
Clearance index K ═ lgOD2-lgOD8/t2-t1
Effects of delayed allergy: on the 10 th day after administration, the abdomen was dehaired to about 3 cm. times.3 cm, the dehaired part was coated with 50. mu.l of 1% dinitrofluorobenzene, on the 14 th day after administration, 10. mu.l of 1% dinitrofluorobenzene was coated on the right ear, the mouse was sacrificed after 24 hours, the ear piece of the same part of both ears was punched out with an 8mm punch, and the weight was weighed, and the difference between the weights of the left and right ear pieces was taken as the swelling degree.
Table 2 experimental results for enhancing immunity (X shi S, n 10)
Note P <0.05, P <0.01 compared to normal group; compared with the model group, # P <0.05, # P <0.01
The experimental result shows that the spleen atrophy caused by hydrocortisone can be obviously antagonized by the hydrocortisone in the examples 1 and 2, and the results suggest that the two have certain immune enhancement effects, the effects of the two are inferior in the examples 3 and 4, and the effects of the two are the worst in the examples 5 and 6, but the effects are better than that of the ant wine and the black ant health-preserving wine. The clearance index K experiment reflects the phagocytic function of kupffer cells of the liver and spleen macrophages, and the delayed allergy is a response depending on cellular immunity. The experimental result shows that the example 1 and the example 2 obviously improve the clearance index and the phagocytosis index of the mice with low immune function and inhibit the delayed allergy of the mice, which indicates that the invention has obvious effect on nonspecific immunity and cellular immunity.
The above are merely embodiments of the present invention, which are described in detail and with particularity, and therefore should not be construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the spirit of the present invention, and these changes and modifications are within the scope of the present invention.
Claims (10)
1. The ant health wine is characterized by comprising the following substances of effective components mixed in white spirit in parts by weight:
2. the ant health wine as claimed in claim 1, which comprises the following materials in parts by weight mixed with the effective components of the white spirit:
3. the ant health wine as claimed in claim 1, wherein the liquor is liquor prepared from grain as raw material, and the alcoholic strength of the liquor is 50-60% v/v.
4. The ant health wine as claimed in claim 1 or 2, wherein the amount of the white spirit is 6-10 times of the total amount of the materials.
5. A method for preparing ant health care wine based on any one of claims 1 to 3, which is characterized by comprising the following steps:
grinding, namely grinding the components in proportion into powder;
soaking, namely soaking the ground material into white spirit to obtain a suspension;
filtering, namely filtering the soaked suspension liquid to obtain a liquid component;
diluting, diluting the filtered liquid component with purified water to alcohol content of 38%;
and (4) packaging, namely canning and packaging the diluted liquid.
6. The method for preparing ant health wine as claimed in claim 5, wherein the soaking temperature is between 12-30 deg.C, and the soaking time is between 20-35 days.
7. The method for preparing ant health wine as claimed in claim 5, wherein the components are ground into powder with mesh number of 20-80.
8. A method for preparing ant health care wine based on any one of claims 1 to 3, which is characterized by comprising the following steps:
proportioning, and obtaining the proportioned and crushed substances;
extracting effective components, namely extracting the effective components of each substance in a proportioning amount by taking white spirit as a solvent;
filtering, and filtering the extracted liquid to obtain a filtrate;
diluting the filtrate to 38% alcohol content;
canning and packaging.
9. The method for preparing ant health wine as claimed in claim 8, wherein the extraction of the effective components is carried out by reflux extraction of the effective components using white spirit as solvent.
10. The method for preparing ant health wine as claimed in claim 8, wherein the alcoholic strength of the white spirit is between 50% and 60% v/v.
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