CN112168917A

CN112168917A - Composition with effect of relieving physical fatigue and application of composition in health wine

Info

Publication number: CN112168917A
Application number: CN202011069386.3A
Authority: CN
Inventors: 魏利夫; 赵洋; 耿华; 王庆; 李春艳
Original assignee: Liaoning Taiyang Pharmaceutical Technology Development Co ltd
Current assignee: Liaoning Taiyang Pharmaceutical Technology Development Co ltd
Priority date: 2020-10-02
Filing date: 2020-10-02
Publication date: 2021-01-05

Abstract

The invention relates to the field of biomedicine, in particular to a composition for relieving physical fatigue and application thereof in health wine. The composition with the effect of relieving physical fatigue comprises barbary wolfberry fruit, Chinese date, dwarf lilyturf tuber, raspberry, ginseng and cervus elaphus linnaeus. The composition has controllable quality and safe use, and can remarkably relieve and improve fatigue symptoms of people; the composition of the invention is suitable for industrial production.

Description

Composition with effect of relieving physical fatigue and application of composition in health wine

Technical Field

The invention relates to a composition, in particular to a composition for relieving physical fatigue and application thereof in health wine.

Background

With the continuous acceleration of life and work rhythm, people are in various tense and busy things for a long time, and the incidence of dizziness, physical weakness, insomnia, poor appetite and other diseases is continuously increased under the influence of a plurality of factors such as living environment and the like. Traditional Chinese medicine puts physical fatigue into categories of 'fatigue', 'lacked', etc. The theory of traditional Chinese medicine considers that the qi consumption caused by labor, namely the consumption of qi, blood, yin and yang caused by labor and movement is the basis of the occurrence of physical fatigue. Su Wen and show that Su Yi Hou Lun (treatise on Su Wen and monograph article) states that liver deficiency, kidney deficiency and spleen deficiency all cause severe restlessness and lassitude in human body, and indicates that fatigue is mainly related to spleen, liver and kidney. The spleen is the source of the generation and transformation of qi and blood, the spleen fails to transport and transform, the generation and transformation of qi and blood are passive, and the muscles of four limbs cannot be nourished; the function of transporting and resolving water-dampness is reduced, producing dampness and collecting phlegm, and phlegm-dampness stagnating in the muscles of limbs, causing lassitude and hypodynamia of the limbs. Liver stores blood and governs tendons, so called "the root of the strike" and "the strike" means fatigue resistance. The liver stores blood and regulates blood volume, and can ensure normal blood distribution during exercise. Kidneys are the innate root, store essence and govern bone and marrow. Kidney essence deficiency and marrow-sea deficiency can lead to listlessness, soreness and weakness of the waist and knees, atrophy and weakness of limbs, fatigue and weakness.

The book Su Wen, Yin Yang congress elephant, the introduction: for the deficiency of the body, it warms and invigorates qi. Qi is true qi and warm in less fire to develop the body. However, the fire can not heat the body, the heat is strong, and the body is strong, so the vacuum is consumed. When the fire is weak and strong, the fire is between the two kidneys, the traditional Chinese medicine considers that the people with 'shape deficiency' are the symptoms of deficiency and impairment of yang and qi; "warming and invigorating qi" refers to the treatment with the drugs having the functions of tonifying yang and benefiting qi; therefore, for yang deficiency accompanied by qi deficiency, the drugs with mild fire should be used to supplement the body's true qi.

Most of traditional medicinal liquor is obtained by infusing medicinal materials into liquor, the prepared medicinal liquor has undefined active ingredients, and the origin, quality and dosage of the medicinal materials, infusion time, infusion temperature and the like can all influence the active ingredients, so that the quality of the medicinal liquor is uncontrollable; in addition, the medicinal liquor may contain tannin components, so that symptoms such as allergy and the like of some patients may be caused, and even the life safety may be threatened.

Disclosure of Invention

The invention aims to provide a composition with the effect of relieving physical fatigue and a preparation method of health care wine using the composition.

In order to achieve the purpose, the solution adopted by the invention is as follows:

the specific method comprises the following steps:

a composition for relieving physical fatigue comprises fructus Lycii, fructus Jujubae, radix Ophiopogonis, Rubi fructus, Ginseng radix, and cornu Cervi Pantotrichum.

The composition comprises the following raw materials in parts by weight:

the composition is used for preparing food with the effect of relieving physical fatigue.

A health wine with effect of relieving physical fatigue is prepared from Chinese liquor with an alcoholic strength of 38-65% vol and any one of the above composition according to a weight ratio of 6-40:1, wherein the alcoholic strength of the health wine is 20-60% vol.

Preferably, the alcohol content of the white spirit can be 42% vol.

As another preferable mode, the alcoholic strength of the health-care wine can be 25-38% vol.

The vol represents the volume ratio.

A health wine for relieving physical fatigue is also prepared from sugar and water.

A preparation method of health wine with the effect of relieving physical fatigue comprises the following steps:

extracting fructus Lycii, fructus Jujubae, radix Ophiopogonis, Rubi fructus, Ginseng radix, and cornu Cervi Pantotrichum with Chinese liquor for 1 or more times, and filtering.

A preparation method of health wine with the effect of relieving physical fatigue comprises the following steps: pulverizing fructus Lycii, fructus Jujubae, radix Ophiopogonis, Rubi fructus, Ginseng radix, cornu Cervi Pantotrichum, adding Chinese liquor, extracting for 1 or more times, filtering, concocting, standing, and centrifuging.

The leaching time is 3-30 days.

Physical fatigue and morbid fatigue can be cited as fatigue.

Physiological fatigue refers to a defensive response to maintain human health. More specifically, the term "fatigue" refers to fatigue occurring in daily activities such as work, housework, leisure activities, and the like, and includes not only physical fatigue but also mental fatigue.

The morbid fatigue refers to fatigue occurring as a symptom of a basic disease such as heart disease, bronchial asthma, hepatitis, anemia, metabolic disease, muscle disease, various infectious diseases, cancer, and the like, and fatigue such as chronic fatigue syndrome, depression due to mental causes, and overtraining syndrome due to exercise.

The health wine can be used for relieving any one of the fatigue.

The formula mainly treats qi tonifying, yang supporting, spleen tonifying, qi benefiting, spleen tonifying, liver tonifying, kidney tonifying and three internal organs tonifying, combines all the ingredients for tonifying without dryness, and can be eaten for a long time. Wherein, the ginseng is a monarch drug which is sweet and slightly bitter in taste, slightly warm in nature, and has the functions of greatly invigorating primordial qi, tonifying spleen and lung, promoting the production of body fluid to quench thirst, soothing the nerves and improving intelligence; the pilose antler is an article with blood and meat emotion, is sweet in taste and salty in property, warms and tonifies kidney yang, assists yang and transforms qi, and is a ministerial of the formula; the medlar and the raspberry are used as assistant drugs and ministerial drugs in the formula for tonifying the kidney and nourishing the liver; the dwarf lilyturf tuber has the functions of nourishing yin and moistening dryness and assisting in processing dryness-heat of ginseng and pilose antler, and is also used as an assistant in the formula; the Chinese dates are used as a guide in the prescription.

The composition has the beneficial technical effects that:

(1) the fatigue symptoms of people are obviously relieved and improved.

(2) The composition has controllable quality and safe use.

(3) The composition and the medicinal liquor prepared from the composition are suitable for industrial production.

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION

The following examples are provided to describe and further illustrate certain embodiments and aspects of the present invention and are not to be considered as limiting the scope of the invention.

Examples

Example 1:

prescription: 30g of medlar, 30g of Chinese date, 20g of dwarf lilyturf tuber, 20g of raspberry, 12g of ginseng, 5g of cervus elaphus linnaeus, 920ml of 42% vol white spirit, 150g of rock sugar and a proper amount of purified water, and the mixture is prepared into 1000 ml.

The preparation method comprises the following steps: putting fructus Lycii, fructus Jujubae, radix Ophiopogonis, Rubi fructus, Ginseng radix, and cornu Cervi Pantotrichum into an extraction tank, adding Chinese liquor for extraction twice, adding 0.57 times of Chinese liquor for the first time, sealing, soaking for 10 days, filtering, and collecting filtrate; adding 0.43 times of Chinese liquor into the residue, sealing, soaking for 10 days, filtering the extractive solution with 200 mesh sieve, removing the residue, and mixing the filtrates;

adding the filtrate and rock candy according to the formula amount into a stirring tank, stirring for 1 hour to dissolve and mix uniformly, adjusting the alcoholic strength to 36% vol with purified water, standing for 24 hours, removing bottom precipitate, centrifuging the prepared solution (14000 r/min), and obtaining the centrifugate.

Example 2:

prescription: 10g of medlar, 10g of Chinese date, 5g of dwarf lilyturf tuber, 5g of raspberry, 3g of ginseng, 1g of cervus elaphus linnaeus, 800ml of 60% vol white spirit, 100g of rock sugar and a proper amount of purified water, and the mixture is prepared into 1000 ml.

The preparation method comprises the following steps: the preparation method comprises the following steps: putting fructus Lycii, fructus Jujubae, radix Ophiopogonis, Rubi fructus, Ginseng radix, and cornu Cervi Pantotrichum into an extraction tank, adding Chinese liquor for extraction twice, adding 0.57 times of Chinese liquor for the first time, sealing, soaking for 15 days, filtering, and collecting filtrate; adding 0.43 times of Chinese liquor into the residue, sealing, soaking for 15 days, filtering the extractive solution with 200 mesh sieve, removing the residue, and mixing the filtrates;

adding the filtrate and rock candy according to the formula amount into a stirring tank, stirring for 3 hours to dissolve and uniformly mix, adjusting the alcoholic strength to 38% vol with purified water, standing for 24 hours, removing bottom sediment, centrifuging the prepared solution (14000 r/min), and obtaining the centrifugate.

Example 3:

prescription: 20g of medlar, 15g of Chinese date, 8g of dwarf lilyturf tuber, 5g of raspberry, 10g of ginseng, 3g of cervus elaphus linnaeus, 900ml of 20 percent vol white spirit, 120g of rock sugar and a proper amount of purified water, and the mixture is prepared into 1000 ml.

The preparation method comprises the following steps: the preparation method comprises the following steps: putting fructus Lycii, fructus Jujubae, radix Ophiopogonis, Rubi fructus, Ginseng radix, and cornu Cervi Pantotrichum into an extraction tank, adding Chinese liquor for extraction twice, adding 0.57 times of Chinese liquor for the first time, sealing, soaking for 15 days, filtering, and collecting filtrate; adding 0.43 times of Chinese liquor into the residue, sealing, soaking for 20 days, filtering the extractive solution with 200 mesh sieve, removing the residue, and mixing the filtrates;

adding the filtrate and crystal sugar of formula amount into a stirring tank, stirring for 2 hr to dissolve, mixing, adjusting alcohol content to 25% vol with purified water, standing for 24 hr, removing bottom precipitate, centrifuging the solution (14000 rpm), and collecting the centrifugate.

Prescription screening test

Test drugs:

drug 1 group: 12g of ginseng and 5g of cervus elaphus linnaeus;

drug 2 group: 30g of medlar, 12g of ginseng and 5g of cervus elaphus linnaeus;

drug 3 group: 20g of raspberry, 12g of ginseng and 5g of cervus elaphus linnaeus;

drug 4 group: 30g of medlar, 20g of raspberry, 12g of ginseng and 5g of cervus elaphus linnaeus;

drug 5 group: 30g of medlar, 20g of dwarf lilyturf tuber, 20g of raspberry, 12g of ginseng and 5g of cervus elaphus linnaeus;

drug 6 group: 30g of medlar, 30g of Chinese date, 20g of dwarf lilyturf tuber, 20g of raspberry, 12g of ginseng and 5g of cervus elaphus linnaeus;

the test animal is male healthy mouse of Kunming species with 2-3 months of age and weight (25 +/-3) g.

The test method comprises the following steps: the experimental animal mice were divided into groups of 12 mice each, and were bred by water immersion for 5 days. The water immersion breeding refers to breeding under the condition that water with the depth of 1.5cm is filled in a plastic cage. In this environment, the mice who escape water cannot be sufficiently put to sleep, cannot adopt a resting posture, and are always mentally and physically in a state of being unable to rest. It is known that individuals begin to die after being water-soaked for 7 or more days, and mice after being water-soaked for 5 days are an animal model that achieves fatigue and death. The mice were gavaged with different drugs at a dose of 10ml/kg daily by gavage 1 time 1 day from day 1 of water immersion feeding, and the control group was given distilled water in equal amount. And measuring various anti-fatigue indexes on the 6 th day.

After 5 days of water immersion, the mice were swim under a load of a weight corresponding to 80% of the body weight, and the time until the nose tip was submerged in water was measured for 10 seconds or more, and the swimming time was recorded for evaluation of the fatigue.

The determination of serum urea nitrogen comprises preparing serum, subjecting the mouse to reagent 30min at the last time, placing the mouse in a swimming box with water depth of 30cm, controlling water temperature at about 30 deg.C, swimming for 90min, taking blood from tail immediately after resting for 30min, and collecting blood about 0.5ml (without anticoagulant). Placing in a refrigerator at 4 deg.C for about 3h, centrifuging at 2500r/min for 15min after blood coagulation, collecting serum and measuring serum urea nitrogen value.

Liver glycogen extraction (1) extraction of liver glycogen, mice were immediately decapitated and sacrificed 30min after the last administration of the reagent, and the liver was taken out, and the attached blood was rapidly aspirated by a filter paper. Weighing about 1g, placing in a mortar, adding a little clean fine sand and 10% trichloroacetic acid 1m, l, and grinding. Then adding 2m, l of 5 percent trichloroacetic acid, and continuing grinding until the liver tissue is fully ground into meat paste. Then centrifuged at 2500r/min for 10 min. Transferring the supernatant into another centrifuge tube, measuring volume, adding 95% ethanol with the same volume, mixing, and standing for 10 min. At this time, glycogen precipitates in the form of flocs. The precipitated solution was centrifuged at 2500r/min for 10 min. The supernatant was discarded and the tube was inverted on filter paper for 2 min. To the precipitate was added 1ml of distilled water, and the precipitate was stirred with a glass rod until dissolved. ② determining hepatic glycogen, namely determining the hepatic glycogen content according to a literature method, namely an anthrone-concentrated sulfuric acid method, repeating each group for 3 times, and taking an average value.

Table 1: exhaustion swimming time

Note: p <0.05, P <0.01 compared to control; Δ P <0.05 compared to drug 1 group.

Table 2: serum urea nitrogen content

Table 3: hepatic glycogen content

And (4) test conclusion: the tests show that the composition prepared by using ginseng and pilose antler as monarch and minister has the effect of relieving physical fatigue, the medlar and raspberry are used as adjuvant and guide, the anti-fatigue effect can be obviously improved, and the dwarf lilyturf tuber and the Chinese date are used as adjuvant and guide, so that the better anti-fatigue effect can be ensured, and the health-care food with better mouthfeel can be obtained.

Test of drug efficacy

Test 1: mouse weight bearing swimming test

72 male KM mice were randomly divided into 6 groups of 12 mice each, and the swimming time was recorded for evaluation of fatigue by the same test method as above, and the results are shown in Table 4.

TABLE 4 exhaustive swimming time

P <0.05, P <0.01, compared to saline group; compared with the solvent group, the delta P is less than 0.05, and the delta P is less than 0.01

And (4) conclusion: compared with the normal saline group and the solvent group, the composition can prolong the exhaustion time of the mouse in the heavy swimming and has statistical significance.

Test 2: effect of acute cerebral ischemia and hypoxia in mice

Taking 72 KM mice, each half of male and female, randomly dividing into 6 groups, namely a normal saline group, a solvent group, a ginsenoside group, an example 1 administration group, an example 2 administration group and an example 3 administration group, continuously administering for 9 days, performing shallow anesthesia by using ether 1h after the last administration, rapidly cutting the ends of the mice along the root parts of ears of the mice, and recording the time that the mice are not gasped in mouth within 5s from the end cutting of the mice, namely the gasping time of the mice in mouth. The results are shown in Table 5.

TABLE 5 Effect on acute cerebral ischemia and hypoxia in mice (χ. + -. s)

And (4) conclusion: compared with the normal saline group and the solvent group, the composition provided by the invention can prolong the gasp time of the mouth opening of a mouse and has statistical significance.

Test 3: mouse autonomic activity test

72 KM mice were selected, and divided into 6 groups at random according to sex and body weight, and the group consisting of physiological saline, solvent, ginsenoside, example 1, example 2 and example 3 were administered continuously for 7 days, and the number of times of movement and standing of the mice was measured 30min, 60min and 120min after the last administration. Mice were acclimated for 3min before the 1 st test, and then the number of movements and stools of the mice within 5min was measured formally. The results are shown in Table 6.

Table 6 influence on the number of autonomic actions in mice (n ═ 14, χ ± s)

And (4) conclusion: compared with the normal saline group and the solvent group, the composition can reduce the activity and standing times of mice at different time points after administration, and has statistical significance.

In conclusion, the composition can obviously prolong the time of mouse weight swimming and acute head and mouth breaking and gasping, can obviously reduce the autonomous activity of the mouse, and shows that the composition saves the consumption of oxygen and energy substances by reducing unnecessary tension and ineffective muscle activity of the body, thereby achieving the effect of resisting fatigue.

Claims

1. A composition with effect of relieving physical fatigue is characterized by comprising wolfberry fruit, Chinese date, dwarf lilyturf tuber, raspberry, ginseng and cervus elaphus linnaeus.

2. The composition according to claim 1, characterized by comprising the following raw materials in parts by weight:

3. the composition of claim 1, wherein the composition is used for preparing a food product with physical fatigue relieving effect.

4. The health wine with the effect of relieving physical fatigue is characterized by being prepared from liquor with the alcoholic strength of 38-65% vol and any one of the mixture ratio of the liquor to the composition in a weight ratio of 6-40: 1.

5. The health wine according to claim 4, wherein the alcoholic strength of the white wine can be 42% vol.

6. The health wine according to claim 4, wherein the alcoholic strength of the health wine is 20-60% vol.

7. The health wine according to claim 4, further comprising sugar and water.

8. The preparation method of the health wine with the effect of relieving physical fatigue is characterized by comprising the following steps:

9. The preparation method of the health wine with the effect of relieving physical fatigue is characterized by comprising the following steps: pulverizing fructus Lycii, fructus Jujubae, radix Ophiopogonis, Rubi fructus, Ginseng radix, cornu Cervi Pantotrichum, adding Chinese liquor, extracting for 1 or more times, filtering, concocting, standing, and centrifuging.

10. The preparation method of the health wine with the effect of relieving physical fatigue is characterized in that the leaching time is 3-30 days.