CN106138680B - Traditional Chinese medicine composition with antioxidant function - Google Patents
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Abstract
The invention relates to a traditional Chinese medicine composition with an antioxidant function, which is prepared from the following traditional Chinese medicines serving as raw materials in parts by weight: 8-25 parts of Chinese date, 4-12 parts of red ginseng, 8-16 parts of radix ophiopogonis, 3-8 parts of schisandra chinensis and 8-20 parts of medlar.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine formula with an antioxidant function, a preparation method and application thereof.
Background
During the metabolism of human body, many free radicals are generated. Researches show that the occurrence and the development of diseases such as senile plaque atherosclerosis, cardiovascular diseases, cancer, peptic ulcer, primary nephropathy, diabetes, bronchial asthma, emphysema and the like are closely related. Antioxidation refers to the abbreviation of antioxidant free radical, Anti-Oxidant. More and more studies show that anti-oxidation is an important step in preventing aging, because free radicals or oxidants break down cells and tissues, affect metabolic functions, and cause various health problems. If the excess oxidative free radicals can be eliminated, many diseases caused by free radicals and related to aging can be prevented.
TCM holds that qi, blood and body fluids are the basic substances that constitute the human body and also the basic substances that maintain the life activities of the human body, and are the products of the physiological activities of the tissues and organs such as the zang-fu organs, meridians and collaterals, and the material basis for the physiological activities of these tissues and organs. Yang qi, yin blood and body fluids are continuously transformed and transported to body surface organs to moisten skin, nourish muscles, moisten hair and resist the invasion of exogenous pathogens, so that the body is strong, muscles are full, skin is fine and elastic, hair is bright, and eyes have spirit.
The invention provides a health food with a traditional Chinese medicine formula, which is prepared by selecting plant raw materials which can be used for health food and are homologous with traditional Chinese medicine food, carrying out dialectical compatibility according to the principle of tonifying qi, enriching blood and promoting the secretion of saliva or body fluid, adopting advanced modern traditional Chinese medicine preparation technology for production and processing, and preparing a traditional Chinese medicine preparation which is convenient for patients to directly take. The traditional Chinese medicine formula consists of Chinese dates, red ginseng, radix ophiopogonis, schisandra chinensis and medlar.
Modern pharmacological studies show that:
the Chinese date has the functions of benefiting qi and nourishing blood:
the red date has the effects of tonifying middle-jiao and Qi, nourishing blood and soothing nerves. The red date has high vitamin content and has a health-care effect on human capillary vessels. 20 red dates, 1 egg and 30 g brown sugar are stewed with water for 1 time per day, so that the health-care tea is suitable for postpartum recuperation and has the effects of benefiting qi and enriching blood.
The Chinese dates have the function of promoting sleep:
red jujube has the functions of invigorating spleen, nourishing blood and tranquilizing. Decocting fructus Jujubae in water after supper for administration; or mixing with Bulbus Lilii and cooking porridge; eating jujube in soup just before sleep can accelerate sleep. 1000 g of fresh red dates are used, washed, denucleated, meat is taken, mashed, added with a proper amount of water, decocted by slow fire, filtered to obtain juice, 500 g of honey is mixed, evenly mixed on fire to obtain jujube paste, and the jujube paste is bottled for later use. The medicine is taken 2 times a day with 15ml each time, and can be used for preventing and treating insomnia.
In addition, the red dates have the effects of supplementing calcium, preventing and treating spermatorrhea, preventing diarrhea and the like.
The Chinese date has the function of beautifying:
the Chinese dates moisten skin, and the folk has the theories of ' eating three Chinese dates one day, being not old for one hundred years ' and ' ensuring good skin ' and adding the Chinese dates in porridge '. 50 g of red dates and 100 g of polished round-grained rice are taken and cooked into porridge, and the porridge is taken warm in the morning and evening and is beneficial to beautifying skin. The reason for this is that a large amount of vitamin B in red dates can promote subcutaneous blood circulation, so that skin and hair are smooth, facial wrinkles are smooth, and the skin is more body-building.
The Chinese date has the function of preventing hair loss:
the red dates have the functions of tonifying spleen and nourishing stomach. The red dates with rich nutrition can prevent hair loss and grow dark and bright hair after being eaten frequently.
The Chinese date has the functions of invigorating stomach and nourishing brain:
the red dates are commonly used in traditional Chinese medicine for nourishing the stomach and tonifying the spleen. For example, when the prescription is used with drugs with strong potency or irritation, red dates are often used to protect the spleen and stomach, and the red dates contain sugar, protein, fat and organic acid, and have the effect of tonifying the brain. The jujube cake prepared from the red jujubes and the flour can nourish the stomach and tonify the brain.
The Chinese date has the effect of reducing senile plaque:
the vitamin C contained in the red dates is a reductive antioxidant substance with strong activity, participates in the reduction process of physiological oxygen in the body, prevents melanin from being precipitated in the body chronically, and can effectively reduce the generation of pigment senile plaques.
The Chinese date has the function of protecting the liver:
the sugar, fat and protein contained in fructus Jujubae are nutritional agent for protecting liver. It can promote liver to synthesize protein, increase the contents of serum red protein and albumin, regulate the ratio of albumin to globulin, prevent blood transfusion reaction, and reduce serum glutamic-pyruvic transaminase level. 50 g of red dates and 90 g of rice are decocted into thick porridge which is beneficial to spleen nourishing and liver protection of hepatitis patients. The Chinese date, the peanut and the rock candy are respectively used for 30 to 50 grams, the peanut is boiled firstly, and then the Chinese date and the rock candy are added for boiling soup, the decoction is taken before sleeping every night, 30 days is a treatment course, and the Chinese date decoction has a certain curative effect on acute and chronic hepatitis and liver cirrhosis.
Legacy application
1. The Chinese dates are used for treating dampness-cold in spleen and stomach, diet reduction, diarrhea and grain indigestion: four or two of rhizoma atractylodis macrocephalae, two or two of rhizoma zingiberis. Chicken's gizzard-membrane is two, and cooked jujube meat is half jin. The four raw materials are used, wherein the white atractylodes rhizome and the chicken's gizzard-membrane are used respectively, each raw material is milled finely and baked, the dried ginger is milled finely and blended with the jujube meat, the mixture is smashed into paste to be used as a small cake, the cake is fried dry on charcoal fire, and the cake is chewed finely when being hollow. ("Yi Xue Zhong Can xi Lu" cake for invigorating spleen)
2. The Chinese dates supplement qi: steaming ten jujubes, removing core, adding ginseng and money, wrapping in a cloth bag, storing in a rice cooker, steaming, smashing uniformly into pills, such as big balls, and collecting for storage. (& ltxing Yuan Lu & gt jujube ginseng pill)
3. Jujube for non-thrombocytopenic purpura: red jujube is eaten three times a day, 10 in each time, until the purple scar is completely removed. Generally, each person needs about one to two jin of red dates. ("Shanghai Zhongyao medicine" (4): 22, 1962)
4. The Chinese date is used for treating hysteria, sadness, crying and inextensibility of women: ten Chinese dates, three or two licorice roots and one liter of wheat. Adding the above three medicines, decocting with six liters of water, and taking three liters of the decoction, wherein the decoction is taken at three times with different temperatures. ("golden Kuiyou slight" in gan Mai Da Zao Tang)
5. Jujube for cough: one hundred and twenty almonds (peeled and sharpened) and one hundred fermented soybeans (decocted to be dry) and forty dry jujubes (pitted). The above three ingredients are smashed into paste, and the pill is as apricot kernel, which is completely swallowed. Seven days and eight degrees, the best one is to do the treatment. (Peltier 'Bixiao Fang')
6. The Chinese date is used for treating consumptive disease, vexation and insomnia: twenty Chinese dates and seven stems of fistular onion stalk. The second medicine is added with three liters of water, boiled for one liter and taken after the dregs are removed. ("Qianjin Fang")
7. The Chinese date is used for treating sudden cardiac pain: one dark plum, two jujubes and seven almonds. Pounded one part, and sent with wine for male and vinegar for female. (& ltMarine Square & gt)
8. Chinese date is used for regurgitation and vomiting: one jujube (removing stones) and one cantharis (removing head and wings) are fed with heat, the cantharis and the hollow food are removed, and the Chinese jujubes are eaten in a white soup. ("gang mu)
9. The Chinese date is used for treating apoplexy, panic, deficiency, palpitation and heavy limbs: seven Chinese dates (core removed) and two green sorghum and maize. The first two medicines are boiled with water three liters and a half liter, the jujube is boiled to take one liter and a half, the dregs are removed, and rice is added to cook the porridge. ("Shengjizhu" book of holy economy)
10. The Chinese date is used for treating lung-abscess, hematemesis and pararrhea: red dates (with the property of being burned in the pit), and Baiyao decocted (calcined) in equal parts. The powder is ground into fine powder, and the rice water is added after two coins are taken each time. (& ltthree reasons prescription & gt Ergrey powder)
Modern pharmacological research shows that the red ginseng has the following effects
1. Red ginseng has an excitatory effect on the central nervous system, whereas it has an inhibitory effect in large amounts. Can enhance the excitation and inhibition process of the higher nerve activity of animals. And can enhance the adaptability of the body to all non-specific stimuli and reduce the fatigue feeling (the root, stem and leaf of red ginseng can prolong the swimming duration of mice).
2. Red ginseng has direct effects on cardiac muscle and blood vessels, and is generally excitatory at low doses and inhibitory at high doses. The 10% red ginseng extract is used for gastric lavage of cats (or rabbits) at a concentration of 1 ml/kg, and has certain effect of improving myocardial weakness. There was a considerable recovery during the rewarming period. It also has antiallergic shock resisting and heart tonifying effects. Ginseng radix Rubri has effect in inhibiting ATPase activity of rat myocardial cell membrane.
3. Strengthening the resistance of the body to harmful factors:
(1) can prevent chicken infected with plasmodium from acute death, and the weight of chicken is gradually increased.
(2) Can inhibit the fever reaction of experimental animals caused by milk injection or vaccine injection.
(3) Can enhance the ability of human body to adapt to temperature change.
(4) When the dog is in a critical state due to massive blood loss or suffocation, the red ginseng preparation is injected immediately, so that the blood pressure reduced to a very low level can be stably raised.
(5) Can prolong the survival time of mice infected by trypanosoma.
(6) Can inhibit systemic inflammatory reaction caused by injection of turpentine or freezing injury of rabbit ear shell.
(7) Promoting healing of some experimental injuries.
(8) Has effects in resisting deficiency of vitamin B1 and B2.
(9) Can accelerate the healing of experimental corneal ulcer of rabbit.
(10) Can reduce the effect of some poisons (benzene, tetraethyl lead, tricresyl phosphate, etc.) on the body.
4. The composition has the effect of reducing blood sugar of animals with hyperglycemia caused by epinephrine; it has effects of improving symptoms of diabetes, slightly reducing blood sugar, and coordinating with insulin.
5. Can promote animal gonad function, and the small white mouse can eat small amount of Ginseng radix Rubri to generate tail lifting phenomenon.
6. At appropriate dosage, the weight of the rabbit can be increased, and the ratio of plasma albumin to globulin is increased.
7. Stimulating hemopoietic organ, and improving anemia.
8. The reticuloendothelial system can be accelerated after a small amount of the medicine is taken for a long time; the opposite effect is obtained when the dosage is too large.
Modern pharmacological research shows that radix Ophiopogonis has the following effects
Effects on the central nervous system
After 0.5ml/10g total amino acids of the dwarf lilyturf tuber are intraperitoneally injected to a mouse for 30 minutes, 30mg/kg sodium pentobarbital is intraperitoneally injected, and the number of animals which disappear after the positive turning reflex is observed. As a result, the total amino acids of the ophiopogon japonicus have obvious synergistic central depressant action (P is less than 0.05), and the total saponins and total sugars of the ophiopogon japonicus have no obvious influence on the sodium pentobarbital with the subliminal hypnotic dose. The 15% ophiopogon japonicus whisker preparation 10ml/kg can inhibit the fever (P <0.01) of rabbits injected with turpentine 2.0ml/kg by stomach irrigation, but the drug administration after the fever has no inhibition effect. The ophiopogon decoction has the effects of tranquilizing, strengthening the sedative effect of chlorpromazine, enhancing the hypnotic effect of pentobarbital sodium, antagonizing the exciting effect of caffeine, and delaying the occurrence time of convulsion, tetanic convulsion and death caused by the huosuling, but can not prevent the animals from dying.
Effects on the cardiovascular System
Effect on ex vivo bufonid toad toad cardiac function: proved by heart perfusion of isolated toads by using a yagi-Hartung method; the total saponins I (crude extract) of radix Ophiopogonis has the strongest effect of strengthening myocardial contractility, while the total saponins II (purer extract) has less effect than the total saponins I, and generally has the effect of strengthening myocardial contractility and simultaneously increasing cardiac output. The large dose of total saponins I and II and total sugar can inhibit heart, and can weaken myocardial contraction force, reduce cardiac output, block atrioventricular conduction, and even stop the heart. The heart rate is generally slightly slowed or unchanged by the total saponins I and II, the total sugar and the total amino acid, and no obvious influence is caused.
Effect on isolated guinea pig cardiac myocardial contraction amplitude: the Langendorff method of guinea pig isolated heart perfusion shows that small dose of radix Ophiopogonis total saponin and total amino acid can enhance myocardial contraction force, increase coronary blood flow, and large dose can inhibit myocardium and reduce coronary blood flow, but the two have no influence on heart rate.
Antiarrhythmic action and its electrophysiological properties: the ophiopogonin 10mg/kg is used for intravenous injection to effectively prevent or resist arrhythmia induced by CHCl3-Adr, BaCl2 and Aco, and the incidence rate of ventricular arrhythmia of 24 h-ligated canine coronary artery is reduced from 87 +/-8 to 57 +/-7%. Electrophysiological experiments show that the ophiopogonin 15mg/kg can obviously reduce Vmax of monophasic action potential of rabbits, and shorten APD10 and APD 50; the total ophiopogonin 50 mu g/ml can also obviously reduce APA and Vmax of transmembrane action potential of guinea pig papillary muscle cells, and obviously shorten APD10 and APD 50; and ERP/APD is increased obviously. Radix Ophiopogonis can be used for improving arrhythmia caused by barium chloride, aconitine, epinephrine, and hypophysin. 2.5g of magnesium sulfate and 20g of radix ophiopogonis are diluted in 500ml of glucose physiological saline for intravenous drip to treat experimental myocardial infarction of dogs, the times of premature beat occurrence for 6h and 24h after medication are compared with those of a control group, and the result shows that the radix ophiopogonis has a certain prevention effect on arrhythmia after myocardial infarction by using small-dose magnesium sulfate.
Effect on cyclic nucleotide metabolism at experimental myocardial infarction: the anterior descending branch of coronary artery is ligated after anaesthesia with New Zealand rabbit to cause acute experimental myocardial infarction, and 15ml of radix Ophiopogonis injection (equivalent to 15g of crude drug) is injected into ear vein. Blood was then collected by direct cardiac puncture immediately after surgery, 15, 30, 60 minutes after surgery, along with controls, and plasma cAMP and cGMP were determined. As a result, 15 minutes after the operation, cAMP and cGMP of the control group continue to increase, the dwarf lilyturf tuber group shows a descending trend (P <0.05), the dwarf lilyturf tuber group is still lower than the control group (P <0.02) after 30 minutes after the operation, and the level returns to the preoperative level after 60 minutes after the operation. Plasma cAMP/cGMP ratio change: the two groups before the operation have no significant difference, and the ratio of the two groups immediately after the operation is reduced; the continuous decrease of the control group is 4.6 +/-1.61 respectively at 15 and 30 minutes after the operation; 4.57 +/-2.01; the level of the radix ophiopogonis in the radix ophiopogonis group is close to the preoperative level and is respectively 6.46 +/-2.12 and 6.48 +/-2.39 (the P values are all less than 0.05), the preoperative level of the radix ophiopogonis group is still maintained after 60 minutes of operation, the control group has an ascending trend, and the two groups have no significant difference. The cAMP and cGMP content in blood plasma after acute myocardial infarction is obviously higher than normal. It is probably a stress response, due to the necrotic dissolution of a large number of cardiomyocytes, releasing cAMP and cGMP, which increases plasma levels and reflects the degree of myocardial damage. Since cGMP increases more significantly than cAMP during myocardial ischemia, the cAMP/cGMP ratio decreases significantly after myocardial infarction. The ophiopogon root can increase the nutritive blood flow of the infarcted myocardium, repair and protect ischemic and anoxic cardiomyocytes more quickly, so that the release of myocardial cGMP and cAMP is reduced, the content of the plasma is reduced, and the ratio of the two is restored to balance.
Effect on animal immune Activity
Effect on mouse normbaric hypoxia tolerance: taking 18-20g of ICR mice, injecting ophiopogonpolysaccharide and ginsenoside (20mg/kg) into the abdominal cavity of 30 ICR mice respectively, adding physiological saline with the same amount into a closed wide-mouth bottle containing sodium lime after 30 minutes, and recording the death time of the mice. As a result, the survival time of mice in the ophiopogonpolysaccharide group is remarkably prolonged compared with that in the control group (P < 0.01).
Effect on immune organ weight: ICR mice are respectively injected with ophiopogonpolysaccharide and ginseng total saponin (dose is 10mg/kg) in the abdominal cavity, the animals are killed by bloodletting after 7 days of continuous administration of the same amount of normal saline, the weight of the animals, the weight of the thymus and the weight of the spleen are weighed, and the thymus index and the spleen index are calculated. As a result, the ophiopogonpolysaccharide can remarkably increase the spleen weight of the mice (P <0.01), and has obvious influence on thymic.
Effect on mouse carbon clearance: taking ICR mice, respectively carrying out intraperitoneal injection on ophiopogonpolysaccharide, ginseng total saponin (10mg/kg) and normal saline, continuously administering for 7 days, and administering tail veins to Chinese carbon ink diluted by 3 times by 0.1ml/10g after the last administration, and respectively calculating the clearance index. The result shows that the ophiopogonpolysaccharide group can remarkably enhance the carbon clearance effect of the mice (P <0.01 ═ P).
Effect on the decrease in mouse leukocyte count caused by cyclophosphamide and 60Cor irradiation: the ophiopogonpolysaccharide 10mg/kg has very obvious effect of resisting leucocyte reduction caused by intraperitoneal injection of 0.4ml cyclophosphamide (5mg/ml) to mice (P is less than 0.01). The same dose of ophiopogonpolysaccharide was intraperitoneally injected continuously for 8 days, the mice were irradiated at a distance of 60cm from an irradiation source (60Cor) at lh after the administration on day 8 at a total dose of 20.64c/kg, the administration was continued for 3 days after the irradiation, and blood was collected from the orbit on day 7 after the irradiation to calculate the white blood cell count. As a result, ophiopogonpolysaccharide can remarkably resist the decrease of white blood cells caused by 60Cor radiation (P <0.01 ═ P).
The ophiopogonpolysaccharide has obvious promotion effect on the formation of hemolysin in mouse serum and has lectin-like effect on rabbit red blood cells.
The dwarf lilyturf tuber water decoction is injected into a mouse in an abdominal cavity, the dosage is equivalent to 12.5g of crude drug/kg, and the weight of the spleen of the mouse can be remarkably increased; enhancing the carbon particle clearance effect of the mice; has remarkable effect of resisting leucopenia caused by cyclophosphamide.
Effects on humoral and cellular immunity: BALB/C young mice were used to pair the test group and the control group, and after 1 month of rearing, 4.O hundred million (0.2ml) sheep cells (SRBC) were injected into each mouse, and blood was periodically taken after injection to measure the antibody, thereby calculating HC 50. The results show that the use of ophiopogon root has the effects of promoting antibody production and delaying antibody regression (P < 0.05). Two sets of BALB/C mouse spleen cell suspensions were used in pairs, 2 with PHA 60. mu.g/ml and 1 without mitogen. After 72H of culture, 3H thymidine is doped, and the lymphocyte transformation stimulation index is measured on a scintillation instrument, and the result shows that the ophiopogon japonicus fibrous root extract has the function of improving the cellular immunity.
Effect on immune function in tumor-bearing mice: the ophiopogon japonicus whisker aqueous solution has obvious improvement (P <0.01 or P <0.05) on white blood cells and T cells of NIH pure line inoculated S180 and EAC cancer cells. The T cell subset can increase Tu% content and decrease Tr% content, and T u/Tr value is higher than that of the control group.
Other effects
The radix ophiopogonis added into the feed for the mice can reduce hydroxyproline in the body. The inhibition rate of monoamine oxidase (MAO-B) in the brain of male mice was 38.6%. The SOD activity in the liver of the male mouse is improved by 45.5 percent. The drosophila service life test also shows that the radix ophiopogonis root hair feed can obviously prolong the service life of drosophila, and indicates that the feed has the aging delaying tendency. Swimming test shows that the ophiopogon root contains saponin, polysaccharide, amino acid, etc. with obvious antifatigue effect.
Modern pharmacological research shows that the schisandra chinensis has the following effects
1. Acting on central nervous system
The schisandra chinensis has obvious sedative effect, and the schisandra chinensis extract and the methanol can reduce the autonomous activity of mice and have analgesic effect and muscle relaxation effect.
2. Liver protection effect
The schisandrin alcohol extract and schisandrin A, schisandrin B, schisandrin C, schisandrin A, schisandrin B and the like have obvious protective effect on animal hepatocyte damage caused by chemical poisons.
3. Has effects on cardiovascular system
Fructus Schisandrae has effect in dilating blood vessel. Schisandrin, schisandrin C, deoxyschizandrin, etc. all have inhibitory effect on mesenteric artery constriction of isolated dog, and can increase isolated heart of rat and anaesthesia dog coronary blood flow.
4. Antiaging effect
According to the records of this Jing, Wu Wei Zi is used to supplement qi, tonify deficiency, strengthen yin and benefit male essence. Schisandrin B and schisanhenol have antioxidant effect, and can scavenge free radicals and inhibit formation of lipid peroxide. In addition, the schisandra chinensis can reduce serum cholesterol and increase the protein content in the brain and liver, which shows that the schisandra chinensis has an anti-aging effect.
5. Acting on the respiratory system
Wu Wei Zi can astringe lung qi and relieve cough and dyspnea. The schisandra chinensis can enhance the function of bronchial epithelial cells of mice with chronic bronchitis.
6. Effect on metabolism and immune function
Fructus Schisandrae can promote synthesis of hepatic glycogen, enhance glycometabolism, and increase synthesis of hepatocyte protein.
7. The anti-ulcer effect of the schisandra chinensis is used for gastric lavage of rats, and can inhibit the generation of stress ulcer, reduce ulcer index and inhibit gastric secretion.
8. Tranquilizing effect
The schisandra chinensis has good nerve-soothing effect, and the insomnia symptom can be well relieved by insisting on drinking the soaked schisandra chinensis water.
Modern pharmacological research shows that the medlar has the following effects
An immunomodulation
The wolfberry fruit is rich in wolfberry fruit polysaccharide, which is water soluble polysaccharide comprising 6 kinds of monosaccharide including arabinose, glucose, galactose, mannose, xylose and rhamnose, and has physiological activity of strengthening nonspecific immunity, raising disease resistance, inhibiting tumor growth and cell mutation.
Anti-aging of antibody
Immunosenescence is closely associated with apoptosis. Lycium Barbarum Polysaccharides (LBP) can obviously improve the phagocytic function of phagocytes and improve the proliferative capacity of lymphocytes.
Three anti-tumor
The lycium barbarum polysaccharide is not only a biological response regulator for regulating immune response, but also can play an anti-cancer role through a nerve-endocrine-immune regulation network.
Four-anti-fatigue
The lycium barbarum polysaccharide can remarkably increase the reserve of mouse muscle glycogen and liver glycogen and improve the total activity of blood lactate dehydrogenase before and after exercise; the increase of blood urea nitrogen of the mouse after strenuous exercise is reduced, and the clearance rate of the blood urea nitrogen after the exercise is accelerated. This shows that lycium barbarum polysaccharides have a very significant effect on fatigue relief.
Five resistance to radiation damage
Fructus Lycii has effects of resisting Y-ray radiation and protecting organism, and can be used as adjuvant medicine for matching with radiotherapy and other anti-tumor treatments, relieving toxic and side effects of radiotherapy, improving curative effect, and protecting organism immunity.
Regulating blood fat
The medlar can effectively reduce the content of triglyceride and cholesterol in the serum of a rat suffering from hyperlipidemia, has obvious functions of reducing the blood fat and regulating lipid metabolism, and has positive effects on preventing cardiovascular diseases.
Seven blood sugar reducing
The lycium barbarum polysaccharide can obviously enhance the activity of superoxide dismutase (SOD) in damaged islet cells, improve the oxidation resistance of the islet cells, relieve the damage of peroxide to the cells and reduce the generation amount of internal dialdehyde, which shows that the lycium barbarum polysaccharide has a certain protection effect on the islet cells.
Eight-purpose blood pressure lowering medicine
The lycium barbarum polysaccharide can reduce the blood pressure of rats (both systolic pressure and diastolic pressure have effects); reduce the content of malondialdehyde and endothelin in blood plasma and blood vessel, increase the release of calcitonin gene-related peptide, and prevent hypertension.
Jiu protection reproductive system
The lycium barbarum polysaccharide can increase the serum sex hormone level of a human mouse with damaged testis; increase the organ coefficient of testis and epididymis, improve the SOD activity of rat testis tissue, reduce the content of malondialdehyde, and restore the damaged testis tissue to be close to normal level.
Ten improve the eyesight
The human retina photoreceptor is composed of retinol and opsin, rich B-carotene in medlar can be converted into vitamin A in human body, and vitamin A can generate retinol, thereby improving vision and preventing macular disease.
Eleven improvement of respiratory tract disease resistance
The fructus Lycii is rich in carotene, can be converted into vitamin A in human body, has the functions of maintaining normal growth and differentiation of epithelial tissue, and can prevent infection of nose, pharynx, larynx and other respiratory tract, and improve respiratory tract disease resistance.
Twelve skin caring and skin moistening effects
Skin aging is mainly caused by free radical oxidation, and lycium barbarum polysaccharide and B-carotene contained in the lycium barbarum are powerful antioxidants, and a powerful antioxidant army is formed by the synergistic effect of trace element selenium and vitamin E contained in the lycium barbarum; in addition, vitamin A can maintain the growth and differentiation of the cattle, prevent skin dryness and hair follicle keratinization, and play the roles of maintaining beauty, keeping young and moistening skin.
The prior art does not disclose the antioxidant function of the traditional Chinese medicine formula formed by the medicines, so that the invention obtains a novel traditional Chinese medicine composition with antioxidant effect and a preparation method thereof through experiments.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine composition with an antioxidant function and a preparation thereof.
The invention also aims to provide a preparation method of the traditional Chinese medicine composition.
In addition, the invention also provides application of the traditional Chinese medicine composition in preparing health-care food with an antioxidant function.
The invention is realized by the following technical scheme:
the traditional Chinese medicine composition is prepared by taking the following traditional Chinese medicines in parts by weight as raw materials:
8-25 parts of Chinese date, 4-12 parts of red ginseng, 8-16 parts of radix ophiopogonis, 3-8 parts of schisandra chinensis and 8-20 parts of medlar.
The preferred traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 15-25 parts of Chinese date, 5-8 parts of red ginseng, 10-14 parts of radix ophiopogonis, 3-6 parts of schisandra chinensis and 15-20 parts of medlar.
The most preferred traditional Chinese medicine composition is prepared by taking the following traditional Chinese medicines in parts by weight as raw materials: 20 parts of Chinese date, 6 parts of red ginseng, 12 parts of dwarf lilyturf tuber, 4 parts of Chinese magnoliavine fruit and 18 parts of Chinese wolfberry.
The components are proportioned according to weight, and can be increased or reduced according to corresponding proportion during production, for example, the mass production can be in kg or t (ton); small-scale formulations may also be in units of g. The weight can be increased or decreased, but the weight proportion of the crude drugs among the components is not changed.
The proportion of the weight proportion is obtained by scientific screening, and for special patients, such as severe or mild patients, obese patients or lean patients, the proportion of the components can be correspondingly adjusted, the increase or decrease is not more than 10 percent, and the drug effect is basically unchanged.
The traditional Chinese medicine composition is prepared by extracting or processing traditional Chinese medicine raw materials consisting of the formula in other modes to prepare a pharmaceutically active substance, and then taking the substance as a raw material, adding a pharmaceutically acceptable carrier if necessary and preparing the composition according to the conventional technology of pharmaceutics. The active substance can be obtained by respectively crushing or extracting traditional Chinese medicine raw materials, or by jointly crushing or extracting traditional Chinese medicine raw materials, or by other methods, such as: the active substances are obtained by crushing, squeezing, grinding, sieving, percolating, extracting, water extracting, alcohol extracting, ester extracting, chromatography and the like, can be in a powder form or an extract form, can be dry extract or fluid extract, and are prepared into different concentrations according to different requirements of preparations.
The traditional Chinese medicine composition, preferably in the form of unit dose pharmaceutical preparation, can be prepared into any pharmaceutical dosage form during preparation of the pharmaceutical preparation.
According to one embodiment of the present invention, the Chinese medicinal composition of the present invention is in the form of any one oral preparation selected from the group consisting of: tablet, sugar-coated tablet, film-coated tablet, enteric-coated tablet, capsule, hard capsule, soft capsule, oral liquid, buccal agent, granule, pill, powder, unguent, pellet, and suspension.
Preferably, the Chinese medicinal composition is an oral liquid.
The traditional Chinese medicine composition can be added with some pharmaceutically acceptable carriers according to needs, and the pharmaceutical preparation can be prepared by adopting the conventional technology of pharmaceutical preparation, such as mixing the pharmaceutically active substance with the pharmaceutically acceptable carriers. The pharmaceutically acceptable carrier is selected from the group consisting of: mannitol, sorbitol, sorbic acid or potassium salt, sodium metabisulfite, sodium bisulfite, sodium thiosulfate, cysteine hydrochloride, thioglycolic acid, methionine, vitamin A, vitamin C, vitamin E, vitamin D, azone, disodium EDTA, calcium sodium EDTA, carbonates of monovalent alkali metals, acetates, phosphates or aqueous solutions thereof, hydrochloric acid, acetic acid, sulfuric acid, phosphoric acid, amino acids, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivatives, cellulose and derivatives thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, propylene glycol, ethanol, Tween 60-80, span-80, beeswax, lanolin, liquid paraffin, cetyl alcohol, gallic acid esters, agar, triethanolamine, basic amino acids, Urea, allantoin, calcium carbonate, calcium bicarbonate, surfactant, polyethylene glycol, cyclodextrin, beta-cyclodextrin, phospholipid material, kaolin, talcum powder, calcium stearate, magnesium stearate and the like.
When the traditional Chinese medicine composition is prepared into a medicament, the medicament with unit dose can contain 0.1-1000mg of the medicinal active substance, and the balance is a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier may be 0.1-99.9% by weight of the total weight of the formulation.
The traditional Chinese medicine composition can be prepared into health-care food according to the needs, and comprises the following components: beverage, biscuit, bread, candy, can, and any food which can be added.
The usage amount of the traditional Chinese medicine composition is determined according to actual conditions when the traditional Chinese medicine composition is used.
The pharmaceutically active substance of the present invention can be prepared by the following method:
(1) extracting fructus Jujubae and fructus Lycii with 7-10 times of water for 2-4 times, each time for 0.5-2 hr, and filtering to obtain water extract;
(2) boiling radix Ophiopogonis and fructus Schisandrae with 6-9 times of water for 2-4 times, respectively for 0.5-2 hr, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16 at 50 deg.C, adding ethanol until ethanol content is 60-90%, stirring, cooling for 4-12 hr or more, collecting precipitate, dissolving the precipitate with water, concentrating under reduced pressure to relative density of 1.04-1.06 at 50 deg.C to obtain extract;
(3) extracting Ginseng radix Rubri with 6-9 times of water for 2-4 times, each for 30-60min, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16 at 50 deg.C, adding ethanol until ethanol content is 60-90%, stirring, cooling for 4-12 hr or more, collecting precipitate, adding water to dissolve the precipitate, concentrating under reduced pressure to relative density of 1.04-1.06 at 50 deg.C to obtain extract;
(4) and (3) mixing the water extract obtained in the step (1), the extract obtained in the step (2) and the extract obtained in the step (3), and concentrating to obtain the medicinal active substance.
Preferably, the preparation method provided by the invention is as follows:
(1) extracting fructus Jujubae and fructus Lycii with 8 times of water for 2 times, 1 hr each time, and filtering to obtain water extractive solution;
(2) boiling radix Ophiopogonis and fructus Schisandrae with 7 times of water for 3 times (1 hr, 45min, 30min respectively), mixing extractive solutions, concentrating under reduced pressure to 50 deg.C relative density of 1.15-1.16, adding ethanol until the ethanol content is 75%, stirring, standing at cold temperature for more than 12 hr, collecting precipitate, adding water to dissolve the precipitate, concentrating under reduced pressure to 50 deg.C relative density of 1.04-1.06, and obtaining extract;
(3) extracting Ginseng radix Rubri with 8 times of water for 3 times, each for 45min, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16 at 50 deg.C, adding ethanol until ethanol content is 75%, stirring, cooling for over 12 hr, collecting precipitate, dissolving the precipitate with water, concentrating under reduced pressure to relative density of 1.04-1.06 at 50 deg.C to obtain extract;
(4) and (3) mixing the water extract obtained in the step (1), the extract obtained in the step (2) and the extract obtained in the step (3), and concentrating to obtain the medicinal active substance.
According to one embodiment of the invention, the obtained pharmaceutically active substance can be further added with water to be prepared into an appropriate oral liquid concentration, and then the oral liquid is subjected to conventional canning, sterilization and light inspection to obtain a finished product. (see FIG. 1)
The application of the medicinal active substance in preparing the health-care food with the anti-oxidation function comprises the following steps:
the following experimental data illustrate the beneficial effects of the present invention:
(I) test materials
1. Test article
Chinese herbal formula (prepared according to the method of the invention example 1, provided by health food research institute of Tianshili institute)
2. Positive control drug
Vitamin C (Sigma Co., batch SLBH4167V)
3. Laboratory animal
ICR mice: SPF grade, 72, female.
The age of the month: of these, 60 mice were aged 10 months old, and 12 mice were younger 3 months old.
Animal sources: provided by the Yangzhou university comparison medical center, the license: SCXK (Su) 2012-0004.
Feeding conditions are as follows: feeding in a barrier animal house, wherein the temperature is 20-25 ℃, the relative humidity is 60%, 10 animals are placed in each cage, the lighting time is 12 hours, the feed and the full-value pellet feed for the mice (purchased from a Qinglong mountain animal breeding farm in Jianning district of Nanjing city, the quality of the pellet feed meets GB 14924.1-2001' Universal quality Standard for Compound feed for laboratory animals), drinking water freely, and changing the padding every day. Facility use license number: SYXK (Su) 2012-0035.
4. Reagent
Malondialdehyde (MDA) test kit, lot number: 20140627, respectively; superoxide dismutase (SOD) test kit, lot No. 20140701; a trace reduced Glutathione (GSH) test kit, lot No.: 20170702; protein carbonyl content test kit, lot number: 20140705, respectively; are all purchased from Nanjing to build a bioengineering institute.
5. Instrument for measuring the position of a moving object
KD-160 electronic scale, Dongguan Bailida health appliances Limited;
BSA124S precision electronic balance (0.1 mg-120 g), Sidoris, Germany;
TDL80-2B desk centrifuge, shanghai an kiosks scientific instruments factory;
Safire2a measuring and reading instrument, TECAN (switzerland).
(II) Experimental method
1. Experimental dosage design basis
The use scheme of the crowd is as follows: the recommended human intake is 50 ml/bottle/day (oral liquid), i.e. 0.83 ml/kg/d.
In the animal experiment dosage design principle about the antioxidant function test method in the evaluation specification of health food safety toxicology, one dosage group is 10 times (mice) or 5 times (rats) of the recommended dosage of a human body, and the other two dosage groups are arranged, and the high dosage is generally not more than 30 times.
According to the background information and comprehensive consideration, the dosage design aiming at the antioxidant effect animal experiment of the traditional Chinese medicine formula is as follows:
TABLE 1 Experimental groups and dosages administered
2. Route and frequency of administration of test article
(1) Test article administration route and administration volume: the administration was by gavage at a dose volume of 0.2ml/10g BW, 1 dose per day.
(2) And the test article is given for a period: for 30 consecutive days.
(3) Preparing a test article: the test article is prepared daily, the animals are weighed once a week, and the dosage is adjusted according to the measured body weight of the animals.
3. Experimental methods
(1) Animal grouping and handling
After the animals are fed for 7 days in an adaptive manner, except young control mice, all aged mice are subjected to eye socket blood sampling, centrifugation is carried out for 10min at 3500r/min under the condition of 4 ℃, serum is separated, the MDA content in the serum is measured by adopting a kit, and then the aged control mice, the vitamin C control mice and the low, medium and high-dose groups of traditional Chinese medicine are immediately divided into 5 groups according to the MDA content, and each group comprises 12 animals.
Grouping animals, performing intragastric administration to corresponding test substances according to the dosage shown in Table 1 every day for 30 days, after intragastric administration, picking eyeball and taking blood, centrifuging at 4 deg.C for 10min at 3500r/min, separating serum, and storing at-20 deg.C for use; preparing liver tissue into 10% homogenate with normal saline, centrifuging at 3500r/min for 20min at 4 deg.C, collecting supernatant, and storing at-20 deg.C for use.
(2) Observation index
Conventional finger mapping: the body weight of the test mice was measured periodically every week.
Serum index: the kit is used for measuring the MDA content, the protein carbonyl content, the GSH content and the SOD activity in blood.
Liver tissue: the kit is used for measuring the MDA content, the protein carbonyl content, the GSH content and the SOD activity in the liver tissue.
4. Statistical method
Data were analyzed using SPSS11.5 software to analyze
Indicating the significance of the difference between the groups compared with the t-test. P<A difference of 0.05 is significant.
5. Results
(1) Influence on body weight
Young control mice gained normal body weight during the trial. The body weight of the aged mice is obviously higher than that of the young mice, the body weight of each group of mice of the tested substances is kept stable during the test period, and the body weight is not obviously influenced by the tested substances (Table 2).
TABLE 2 influence of gavage administration of Chinese medicinal composition on body weight of aged mice for 1 month (M + -SD)
(2) Influence on serum biochemical indexes
Compared with the young control mice, the serum MDA and protein carbonyl level of the aged mice are obviously increased (P <0.05), the SOD activity is obviously reduced (P <0.05), and the GSH level is also partially reduced. The traditional Chinese medicine formula with high dosage has obvious improvement effect (P is less than 0.05, P is less than 0.01) on serum MDA and protein carbonyl level and SOD activity of aged mice, and the traditional Chinese medicine formula with medium and low dosage also has partial improvement effect. The 3 dosage groups of the traditional Chinese medicine formula have no obvious influence on the average of serum GSH water of the aged mice (Table 3).
TABLE 3 influence of gavage administration of Chinese medicinal composition on biochemical indexes of oxidation of serum of aged mice for 1 month (M + -SD)
*P<0.05, compared to YC group;#P<0.05，##P<0.01, compared to the OC group.
(3) Influence on biochemical indexes of liver
Compared with young control mice, the liver MDA and protein carbonyl level of the aged mice are obviously increased (P <0.01), and the GSH level and SOD activity are obviously reduced (P < 0.01). The traditional Chinese medicine formula with high dosage has obvious improvement effect (P is less than 0.05, P is less than 0.01) on the MDA and protein carbonyl level of the liver of the aged mice and the SOD activity, and the traditional Chinese medicine formula with medium and low dosage also has partial improvement effect. The 3 dosage groups of the traditional Chinese medicine formula have no obvious influence on the average of GSH water level of the liver of the aged mice (Table 4).
TABLE 4 influence of gavage administration of Chinese medicinal composition on biochemical indexes of liver oxidation (M + -SD) of aged mice for 1 month
**P<0.01, compared to YC group;#P<0.05，##P<0.01, compared to the OC group.
(III) conclusion
In the experiment, a natural aging mouse is selected, and the in-vivo antioxidation of the traditional Chinese medicine formula is investigated by investigating MDA, GSH, protein carbonyl level and SOD activity in serum and liver tissues. The traditional Chinese medicine formula is provided with 3 high (16.8ml/kg), medium (8.4ml/kg) and low (4.2ml/kg) dose groups which are respectively equal to 20, 10 and 5 times of the dose of human beings, the continuous gavage administration is carried out for 1 month, and the reagent kit is adopted to measure the biochemical indexes related to oxidation in serum and liver. The result shows that the product has obvious improvement effect on serum, liver MDA, protein carbonyl level and SOD activity of the aged mice after continuous intragastric administration for 1 month, and the product has certain in vivo antioxidation effect according to the evaluation standard of health food inspection and evaluation technical specification (2003 edition).
Description of the drawings:
FIG. 1 is a schematic flow chart of a preparation method according to one embodiment of the present invention.
The specific implementation mode is as follows:
the invention is further illustrated by the following examples, which are not to be construed as limiting the invention thereto.
Example 1
Extracting fructus Jujubae 20 parts and fructus Lycii 18 parts with 8 times of water by boiling for 2 times, each time for 1 hr, and filtering to obtain filtrate. Extracting radix Ophiopogonis 12 parts and fructus Schisandrae 4 parts with 7 times of water for 1 hr, 45min and 30min respectively for 3 times. Mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16(50 deg.C), adding ethanol until ethanol content is 75%, stirring, and cooling for over 12 hr. Collecting precipitate, adding water to dissolve the precipitate, and concentrating under reduced pressure to relative density of 1.04-1.06(50 deg.C). Extracting Ginseng radix Rubri 6 parts with 8 times of water for 3 times, each for 45min, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16(50 deg.C), adding ethanol until ethanol content is 75%, stirring well, and cooling for over 12 hr. Collecting precipitate, adding water to dissolve the precipitate, and concentrating under reduced pressure to relative density of 1.04-1.06(50 deg.C).
Mixing the extractive solutions of fructus Jujubae, fructus Lycii, radix Ophiopogonis, Ginseng radix Rubri, and fructus Schisandrae, concentrating, drying to obtain pharmaceutically active substance, adding the pharmaceutically active substance, blending by conventional technique, and packaging. Sterilizing, inspecting by lamp, and packaging to obtain the traditional Chinese medicine formula oral liquid preparation.
Example 2
Extracting fructus Jujubae 8 parts and fructus Lycii 20 parts with 7 times of water for 30min for 4 times, and filtering to obtain filtrate. Extracting radix Ophiopogonis 10 parts and fructus Schisandrae 8 parts with 6 times of water for 30min for 4 times. Mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16(50 deg.C), adding ethanol until ethanol content is 60%, stirring, and cooling for over 12 hr. Collecting precipitate, adding water to dissolve the precipitate, and concentrating under reduced pressure to relative density of 1.04-1.06(50 deg.C). Extracting Ginseng radix Rubri 12 parts with 6 times of water for 30min for 4 times, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16(50 deg.C), adding ethanol until ethanol content is 60%, stirring well, and cooling for over 10 hr. Collecting precipitate, adding water to dissolve the precipitate, and concentrating under reduced pressure to relative density of 1.04-1.06(50 deg.C).
Mixing the extractive solutions of fructus Jujubae, fructus Lycii, radix Ophiopogonis, Ginseng radix Rubri, and fructus Schisandrae, and adding water. Heating and boiling, and packaging. Sterilizing, inspecting by lamp, and packaging to obtain the traditional Chinese medicine formula oral liquid preparation.
Example 3
Extracting fructus Jujubae 15 parts and fructus Lycii 8 parts with 10 times of water for 2 times, each time for 2 hr, and filtering to obtain filtrate. Extracting radix Ophiopogonis 16 parts and fructus Schisandrae 3 parts with 9 times of water for 2 times each for 2 hr. Mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16(50 deg.C), adding ethanol until ethanol content is 90%, stirring, and cooling for over 10 hr. Collecting precipitate, adding water to dissolve the precipitate, and concentrating under reduced pressure to relative density of 1.04-1.06(50 deg.C). Extracting Ginseng radix Rubri 4 parts with 9 times of water for 2 times, each time for 1 hr, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16(50 deg.C), adding ethanol until ethanol content is 90%, stirring well, and cooling for more than 4 hr. Collecting precipitate, adding water to dissolve the precipitate, and concentrating under reduced pressure to relative density of 1.04-1.06(50 deg.C).
Mixing the extractive solutions of fructus Jujubae, fructus Lycii, radix Ophiopogonis, Ginseng radix Rubri, and fructus Schisandrae, concentrating, adding appropriate adjuvant, and making into tablet by conventional method.
Example 4
Extracting fructus Jujubae 10 parts and fructus Lycii 10 parts with 9 times of water by boiling for 3 times, each time for 45min, and filtering to obtain filtrate. Extracting radix Ophiopogonis 8 parts and fructus Schisandrae 4 parts with 8 times of water for 45min for 3 times. Mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16(50 deg.C), adding ethanol until ethanol content is 80%, stirring, and cooling for more than 4 hr. Collecting precipitate, adding water to dissolve the precipitate, and concentrating under reduced pressure to relative density of 1.04-1.06(50 deg.C). Extracting 5 parts of Ginseng radix Rubri with 9 times of water for 3 times, each time for 45min, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16(50 deg.C), adding ethanol until ethanol content is 80%, stirring well, and cooling for over 12 hr. Collecting precipitate, adding water to dissolve the precipitate, and concentrating under reduced pressure to relative density of 1.04-1.06(50 deg.C).
Mixing the extractive solutions, concentrating, adding adjuvant, and making into capsule by conventional method.
Example 5
The pharmaceutically active substance of example 1 was mixed with any one of foods to prepare a health food.
Example 6
The pharmaceutically active substance of example 1 was added with water and sugar to prepare a health drink.
Example 7
The pharmaceutically active substance of example 1 was added to flour and made into biscuits.
Claims (9)
1. A traditional Chinese medicine composition with an antioxidant function is characterized by being prepared from the following traditional Chinese medicines in parts by weight: 15-25 parts of Chinese date, 5-8 parts of red ginseng, 10-14 parts of radix ophiopogonis, 3-6 parts of schisandra chinensis and 15-20 parts of medlar.
2. The traditional Chinese medicine composition of claim 1, which is prepared from the following traditional Chinese medicines in parts by weight: 20 parts of Chinese date, 6 parts of red ginseng, 12 parts of dwarf lilyturf tuber, 4 parts of Chinese magnoliavine fruit and 18 parts of Chinese wolfberry.
3. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition is any one of the following oral preparations: sugar-coated tablet, film-coated tablet, enteric-coated tablet, hard capsule, soft capsule, oral liquid, buccal agent, granule, pill, powder, and pellet.
4. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition is an oral liquid.
5. The traditional Chinese medicine composition of claim 1, which can be further prepared into any one of the following health foods: beverage, biscuit, bread, candy, and can.
6. The preparation method of the traditional Chinese medicine composition of claim 1, which is characterized by comprising the following steps:
(1) extracting fructus Jujubae and fructus Lycii with 7-10 times of water for 2-4 times, each time for 0.5-2 hr, and filtering to obtain water extract;
(2) boiling radix Ophiopogonis and fructus Schisandrae with 6-9 times of water for 2-4 times, each for 0.5-2 hr, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16 at 50 deg.C, adding ethanol until ethanol content is 60-90%, stirring, cooling for 4-12 hr or more, collecting precipitate, dissolving the precipitate with water, concentrating under reduced pressure to relative density of 1.04-1.06 at 50 deg.C to obtain extract;
(3) extracting Ginseng radix Rubri with 6-9 times of water for 2-4 times, each for 30-60min, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16 at 50 deg.C, adding ethanol until ethanol content is 60-90%, stirring, cooling for 4-12 hr or more, collecting precipitate, adding water to dissolve the precipitate, concentrating under reduced pressure to relative density of 1.04-1.06 at 50 deg.C to obtain extract;
(4) and (3) mixing the water extract obtained in the step (1), the extract obtained in the step (2) and the extract obtained in the step (3), and concentrating to obtain the pharmaceutically active substance of the traditional Chinese medicine composition.
7. The preparation method of the traditional Chinese medicine composition of claim 6, which is characterized by comprising the following steps:
(1) extracting fructus Jujubae and fructus Lycii with 8 times of water for 2 times, 1 hr each time, and filtering to obtain water extractive solution;
(2) boiling radix Ophiopogonis and fructus Schisandrae with 7 times of water for 3 times (1 hr, 45min, 30min respectively), mixing extractive solutions, concentrating under reduced pressure to 50 deg.C relative density of 1.15-1.16, adding ethanol until the ethanol content is 75%, stirring, standing at cold temperature for more than 12 hr, collecting precipitate, adding water to dissolve the precipitate, concentrating under reduced pressure to 50 deg.C relative density of 1.04-1.06, and obtaining extract;
(3) extracting Ginseng radix Rubri with 8 times of water for 3 times, each for 45min, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.15-1.16 at 50 deg.C, adding ethanol until ethanol content is 75%, stirring, cooling for over 12 hr, collecting precipitate, dissolving the precipitate with water, concentrating under reduced pressure to relative density of 1.04-1.06 at 50 deg.C to obtain extract;
(4) and (3) mixing the water extract obtained in the step (1), the extract obtained in the step (2) and the extract obtained in the step (3), and concentrating to obtain the pharmaceutically active substance of the traditional Chinese medicine composition.
8. The method for preparing a Chinese medicinal composition according to claim 7, wherein the obtained pharmaceutically active substance can be further formulated with water to give an oral liquid with a suitable concentration, and then the oral liquid is subjected to conventional canning, sterilization and light inspection to obtain a finished product.
9. The use of the Chinese medicinal composition of claim 1 in the preparation of medicaments and health foods with antioxidant function.
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| CN102860496A (en) * | 2012-10-09 | 2013-01-09 | 三普药业股份有限公司 | Oxidation-resisting health-care food for improving immunity and preparation method thereof |
| CN102860511A (en) * | 2012-10-09 | 2013-01-09 | 三普药业股份有限公司 | Antioxidant anti-anoxia health food and preparation method thereof |
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| CN102860496A (en) * | 2012-10-09 | 2013-01-09 | 三普药业股份有限公司 | Oxidation-resisting health-care food for improving immunity and preparation method thereof |
| CN102860511A (en) * | 2012-10-09 | 2013-01-09 | 三普药业股份有限公司 | Antioxidant anti-anoxia health food and preparation method thereof |
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| Title |
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| 生脉饮抗氧化作用对红细胞膜保护作用的研究;余庆皋;《中国现代医学杂志》;20030531;第13卷(第10期);64-66 * |
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