CN109793885A - It is a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia - Google Patents
It is a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia Download PDFInfo
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- CN109793885A CN109793885A CN201910186658.9A CN201910186658A CN109793885A CN 109793885 A CN109793885 A CN 109793885A CN 201910186658 A CN201910186658 A CN 201910186658A CN 109793885 A CN109793885 A CN 109793885A
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, (1) by a variety of medicinal and edible plant ultrasonications with blood circulation-promoting functions, it forms plant and is crushed liquid (2) and is crushed in liquid to plant and cellulase degradation is added obtains plant enzymolysis liquid (3) plant complex enzyme zymohydrolysis is added to plant enzymolysis liquid obtaining micromolecular polysaccharide vegetation water (4) hemoglobin powder is added into micromolecular polysaccharide vegetation water, deer placenta powder, which is digested, filters micromolecule polypeptide just liquid using flame filter press for liquid (5) at the beginning of micromolecule polypeptide, it obtains polypeptide refined filtration liquid (6) auxiliary material is added into polypeptide refined filtration liquid obtaining complex polypeptide oral solution or complex polypeptide agent.This method changes the preparation method for being in the prior art simply added the finished product peptide native after production with medicinal raw material, the complex polypeptide prepared in this way can effectively improve erythrocyte, and the onset time of this complex polypeptide alleviation anaemia is short, plays the role of prevention or alleviates anaemia.
Description
Technical field
The present invention relates to field of health care products, and in particular to a kind of for preventing or alleviating the preparation side of the complex polypeptide of anaemia
Method.
Background technique
Anaemia is the subnormal clinical syndrome of peripheral red blood cells capacity caused by a variety of causes.In a constant volume
Red blood cell count(RBC) hemoglobin content and hematocrit are below arm's length standard person in blood circulation.Wherein most with hemoglobin
It is important.According to the statistics of the World Health Organization: there are about the different degrees of anaemias of 3,000,000,000 people in the whole world.Adult's anemia is to human body shadow
Very little is rung, but heavier anaemia can cause the long-term blood supply insufficiency of tissue, organ, influence the function of each histoorgan, may cause
Serious consequence.Number dead due to suffering from anaemia and being led to various diseases is up to ten million every year.Wherein there are about 50% Anemic patients is
Caused by asiderosis.Hypoferric anemia (iron deficiency anemia, IDA) refers to that storage iron is not in vivo
Foot influences a kind of caused microcytic hypochromic anemia of hemoglobin synthesis, and IDA is generally existing in the whole world, WHO's
Data shows to influence the population in the whole world 30%, especially children (especially infant) and pregnant woman, accounts for its sum respectively
50% and 40%.Chinese 4th nutrition survey the result shows that, Chinese residents hypoferric anemia illness rate is 20.1%, medium and small
In 2 years old infant, greater than 60 years old the elderly and the 15-50 years old women of child-bearing age hypoferric anemia illness rate be respectively 31.1%,
29.1% and 19.9%, hypoferric anemia has become one of most common deficiency disease of China.Therefore for hypoferric anemia
The research of prevention and treatment is of great significance.
Red blood cell number and content of hemoglobin can be improved in hemepeptide according to the study, has prevention and treatment to make hypoferric anemia
With.Hemepeptide is mainly by being degraded to obtain the macromolecular of hemoglobin.Hemoglobin is to be responsible for delivery in higher organism body
A kind of protein (Hemoglobin, Hb/HGB) of oxygen.Hemoglobin (Hb) by globin and heme in conjunction with and
At.It is exactly because wherein containing heme that red, which is presented, in blood.The abundance of hemoglobin, present technology
How more researchs extract polypeptide from livestock blood, such as:
Patent CN201210516085 discloses that a kind of process using pig blood production peptide native, this method pass through depth
The impurity filtered off except in pig blood is spent, the purity of final products peptide native is improved.
Patent CN2012103571509 discloses a kind of side for extracting by duck blood and having the active peptide of specified molecular weight
Method is realized retention given activity peptide by way of reverse osmosis ultrafiltration grading purification, single-stage enzyme has mainly been used to degrade.
Patent CN2011102863613 discloses a kind of method for improving enzymatic hydrolysis livestock blood red blood cell yield, uses
Specific enzyme 1122 carries out single-stage degradation.
It is one kind using duck blood as raw material small molecule hemepeptide heme iron and blood plasma egg that patent CN2012103573557, which is disclosed,
White production technology, the main purpose of this technique are that small molecule hemepeptide, ferroheme, plasma protein are extracted by duck blood,
Alkali protease degradation is first passed through in enzyme degradation process, in the processing side to degrade together by neutral proteinase and flavor protease
Method also uses acetic acid, ethyl alcohol and clear water washing purification process.
It not is it is obvious that this is because whether hematinic not only will that effect, which is used alone, in the polypeptide that above method is extracted
The effect that can be absorbed by the body and be absorbed and utilized plays a very important role to anaemia is improved.It is also disclosed in the prior art by blood
The product that peptide element and medicinal raw material are prepared, if patent CN99125047.8 is disclosed from extracting peptide native in poultry, processing
The step of include blood collection-DC separation-level-one conversion-second level conversion-peptide native original powder-preparation-product technical process, lead to
It is that the substances such as peptide, amino acid obtain peptide native original powder that endopeptidase, exopeptidase, which are crossed, by cell state biodegrade, and this peptide native is former
Powder is bronzing, although having micro- light bitter taste without peculiar smell, influences the mouthfeel and sense organ of product.The product of this patent disclosure
It is then degradation peptide native is prepared with medicinal raw material again.But the possible mouthfeel of finally obtained product of the reason of because of preparation method
It is bad.The peptide native and medicinal raw material of preparation are easy configuration, so that the activity of the active constituent of peptide native and medicinal raw material
Ingredient fails to play more effective effect.
Summary of the invention
To achieve the goals above, the present invention provides a kind of for preventing or alleviating the preparation side of the complex polypeptide of anaemia
Method, this method change the preparation method for being in the prior art simply added the finished product peptide native after production with medicinal raw material,
The complex polypeptide prepared in this way can effectively improve erythrocyte, and when the action of this complex polypeptide alleviation anaemia
Between it is short, play the role of prevention or alleviate anaemia.
To achieve the above object, present invention employs the following technical solutions:
It is a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, obtained by following preparation steps:
(1) 5-15 parts of water of addition after 1 part of a variety of medicinal and edible plant with blood circulation-promoting functions is mixed will be calculated by weight,
2-10min is extracted in ultrasonication under the conditions of 1600-2500HZ, is then cooled to 35-45 DEG C, is carried out ultrasonication again, reach
Clasmatosis, cytoplasm dissolution form plant and are crushed liquid；
(2) it is crushed in liquid to plant and enters water, until solid-to-liquid ratio is 1:10-20, constant temperature is added cellulase and is digested to 55 DEG C,
Enzymolysis time is 1-4h, so that plant cell wall digests, obtains plant enzymolysis liquid；
(3) plant complex enzyme zymohydrolysis 4-10h is added to plant enzymolysis liquid, keeps constant temperature, solution is then boiled into 5min, enzyme deactivation,
Obtain micromolecular polysaccharide vegetation water；
(4) hemoglobin powder, deer placenta powder are added into micromolecular polysaccharide vegetation water, then 10-20 times of addition water to solid,
Uniformly mixed compound protein liquid, is added protease into compound protein liquid, is digested as liquid at the beginning of micromolecule polypeptide；
(5) liquid at the beginning of micromolecule polypeptide is cooled to 45-65 DEG C, liquid at the beginning of filtering micromolecule polypeptide using flame filter press removes filter
Slag obtains polypeptide refined filtration liquid；
(6) auxiliary material is added into polypeptide refined filtration liquid, sterilizes, obtains compound in 115-121 DEG C after formulated, sterilizing, filling and sealing
Polypeptide oral liquor or Direct spraying are dry, obtain complex polypeptide agent.
The plant degradation of integration of drinking and medicinal herbs is micromolecular polysaccharide vegetation water by the method disclosed by the invention for preparing complex polypeptide
The hemoglobin powder prepared in advance, deer placenta powder are added afterwards, by digesting, while hemoglobin powder of degrading, deer placenta powder, it generates
Small-molecule active substance, and the molecular weight of all the components is controlled by filtering together with micromolecular polysaccharide vegetation water, guarantee activity
Ingredient can be bonded to each other.
Compared with prior art, the present invention having the following beneficial effects:
1, complex polypeptide consists of three parts: hemoglobin powder, deer placenta powder, micromolecular polysaccharide vegetation water, hemoglobin powder mention
For main active, deer placenta powder promotes blood circulation, and increases the absorption of body, and micromolecular polysaccharide vegetation water fills blood, energy
It enough realizes multi-faceted cooperation, prevents anaemia, while being capable of rapid recovery Anemia.
2, hemoglobin powder and deer placenta powder pass through enzyme degradation simultaneously, reduce reaction step, unified degradation effect, mistake
Molecular weight is controlled in close range after filter, is easily absorbed by the human body, effect each other is enhanced.
Specific embodiment
Below by specific embodiment, the present invention is described in further detail:
It is a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, obtained by following preparation steps:
(1) 5-15 parts of water of addition after 1 part of a variety of medicinal and edible plant with blood circulation-promoting functions is mixed will be calculated by weight,
2-10min is extracted in ultrasonication under the conditions of 1600-2500HZ, is then cooled to 35-45 DEG C, is carried out ultrasonication again, reach
Clasmatosis, cytoplasm dissolution form plant and are crushed liquid；
(2) it is crushed in liquid to plant and enters water, until solid-to-liquid ratio is 1:10-20, constant temperature is added cellulase and is digested to 55 DEG C,
Enzymolysis time is 1-4h, so that plant cell wall digests, obtains plant enzymolysis liquid；
(3) plant complex enzyme zymohydrolysis 4-10h is added to plant enzymolysis liquid, keeps constant temperature, solution is then boiled into 5min, enzyme deactivation,
Obtain micromolecular polysaccharide vegetation water；
(4) hemoglobin powder, deer placenta powder are added into micromolecular polysaccharide vegetation water, then 10-20 times of addition water to solid,
Uniformly mixed compound protein liquid, is added protease into compound protein liquid, is digested as liquid at the beginning of micromolecule polypeptide；
(5) liquid at the beginning of micromolecule polypeptide is cooled to 45-65 DEG C, liquid at the beginning of filtering micromolecule polypeptide using flame filter press removes filter
Slag obtains polypeptide refined filtration liquid；
(6) auxiliary material is added into polypeptide refined filtration liquid, sterilizes, obtains compound in 115-121 DEG C after formulated, sterilizing, filling and sealing
Polypeptide oral liquor or Direct spraying are dry, obtain complex polypeptide agent.
A variety of medicinal and edible plants with blood circulation-promoting functions in step (1), according to dry weight calculating include: 10%-30% Radix Angelicae Sinensis,
30%-50% jujube, 20%-50% fructus lycii, 10%-30% rhizoma polygonati.
The weight for the cellulase that step (2) is added is calculated as 2%-7% according to medicinal and edible plant dry weight.
The weight of addition plant complex enzyme in step (3) is calculated as 5%-10% according to medicinal and edible plant dry weight.
The hemoglobin of addition in step (4) and the weight of deer placenta powder are calculated as medicine according to medicinal and edible plant dry weight
1-5 times for eating homologous plant weight, wherein the weight ratio of hemoglobin powder and deer placenta powder is 5-10:1.
Enzymatic hydrolysis in step (4) comprises the following specific steps that: NaOH is added in compound protein liquid, adjusts pH to 9.0, it will be molten
Liquid is placed in 60 DEG C of isoperibol, and the alkali protease of dry matter meter 0.1%-0.5% is added, and maintains 2-6h, will be through parlkaline egg
The compound protein solution of white enzyme enzymatic hydrolysis, adjusts pH to 7.0, and as 50 DEG C of isoperibol, dry matter meter 0.03%-0.1% is added
Flavor protease, digest 0.5h-1h, after boil maintain 2-5min enzyme deactivation, as required micromolecule polypeptide just liquid.
The filtering comprises the following specific steps that: the primary filtrate of complex polypeptide is crossed through ultrafiltration membrane, membrane molecule interception amount
For ten thousand Da film of 1-5,1-2KDa film process is used after being disposed.
The auxiliary material is according to polypeptide essence filtrate volume meter, comprising: 0.11g/L taurine, 2-10g/L fructose syrup, 0.05-
0.1g/L peppermint.
The auxiliary material is according to polypeptide essence filtrate volume meter, further includes: 0.1%-1% Momordia grosvenori aglycone and 0.1-0.8 ‰ are edible
Essence.
Deer placenta: deer placenta deer tire has the effect of " kidney-reinforcing Yang-strengthening, qi-restoratives production of sperm " in traditional Chinese medicine, also commonly uses civil
Make tonic.Modern research shows that placenta contains protein, promoting sexual gland hormone, prolactin, lysozyme, polysaccharide, urokinase
And several kinds of mineral elements and vitamin etc., there is growth promotion, stimulating milk secretion, improve the multiple functions such as immune, have very to female ovary
Good adjustment effect is a kind of ideal immunomodulator and nutrient and healthcare products.It is more using small molecule is formed after its enzymatic hydrolysis
Peptide, adjusts female sex hormone, and help maintains ovary, can promote alleviation anaemia, work well to female anemia.
Radix Angelicae Sinensis: it enriches blood；Promoting blood circulation；Menstruction regulating and pain relieving；Moisturizing dryness and lubricating intestine.The main all cards of the deficiency of blood；Irregular menstruation；Menostasis；Dysmenorrhea；Abdominal mass knot
It is poly-；Metrorrhagia and metrostaxis；Asthenia cold abdominalgia；Flaccidity syndrome and arthralgia syndrome；Numbness；Intestines are dry and shit is difficult；Weight after dysentery with bloody stool；Ulcer sores；Injury from falling down.
Jujube: jujube warm-natured, taste warp sweet in flavor has tonifying middle-Jiao and Qi, nourishing blood and tranquilization mitigates the function of pharmacological property.Modern pharmacology
The study found that jujube can make oxygen content enhancing in blood, nourish systemic cell, it is a kind of tonic that drug effect mitigates.
Fructus lycii: nourishing the liver, nourshing kidney, moistening lung.Wolfberry leaf: qi-restoratives strengthening the essence, heat-clearing improving eyesight.
Rhizoma polygonati: boosting qi and nourishing yin, invigorating the spleen, moistening lung, kidney-nourishing；For deficiency of spleen-QI and stomach-QI, fatigue and asthenia, deficiency of stomach-Yin, dry deficiency of food,
Deficiency syndrome of the lung cough caused by dryness, overstrain cough hemoptysis, asthenia of essence and blood, soreness and weakness of waist and knees, poliosis, Heat Diabetes.
Porcine hemoglobin: being to convert small molecule for the globin in pig blood using pig blood as the biologically active peptide of protein sources
Peptide.Small molecule pig blood peptide not only has good dissolubility, low viscosity, anti-gel formative, but also digests and assimilates in vivo fast.2
The low peptide of~3 amino acid composition has the better absorbent properties of specific ionization amino acid, and the oligopeptides and polypeptide in diet can be complete
Ground causes the biological effect of body as biologically active peptide by intestinal absorption in tissue level.Moreover, it also have it is low
Antigenicity will not generate allergic reaction, additionally have the physiological activity such as fat metabolism therefore will be in haemocyte using pig blood
Hemoglobin be decomposed into globin and ferroheme, and globin is digested into biologically active peptide matters in turn, warp
Small white mouse is crossed experiments have shown that having the function of quickly treating and improving anaemia.The micromolecule polypeptide of hemoglobin enzymatic hydrolysis, can be with
Human body free iron is combined in time, forms erythrocyte, improves Anemia.
The present invention will have promoting blood circulation using Chinese traditional medicine principle, and the substances such as fructus lycii, jujube, rhizoma polygonati, the Radix Angelicae Sinensis of blood tonification effect pass through
Clasmatosis digests small analysis active polysaccharide, then digests jointly with hemoglobin, deer placenta, digests into complex polypeptide substance.Chinese holly
The substances such as Qi, jujube, rhizoma polygonati, Radix Angelicae Sinensis have kidney tonifying, help absorption of nutrient ingredients, while active material and hemoglobin are common
Enzymatic hydrolysis, when forming complex polypeptide, small molecule active ingredient is the more conducively shape of the new hemoglobin of human body in conjunction with hemepeptide
At anaemia problem is treated and treated in help.
Hemoglobin powder of the invention can be made by the following method:
S1, dissolving step: porcine hemoglobin is poured into enzymatic vessel, and 5~15 times of weight of purified water is added, and is sufficiently stirred sufficiently
Dissolution.
S2, enzymolysis step: being added 2~6% alkali protease of porcine hemoglobin dry weight, digests 2~8 hours, same to time control
40~60 DEG C of temperature in enzyme digestion reaction tank processed, pH value are 6.5~8.5；
S3, filtration step: enzyme digestion reaction tank material is heated to boiling, and then slightly boiled 5 to 15 minutes, protease is made to lose activity
Afterwards, enzyme digestion reaction tank is cooled to 45~65 DEG C, then uses flame filter press filter material, removed filter residue, it is molten to obtain hemepeptide
Liquid；
S4, decolorization process: the active carbon of porcine hemoglobin dry weight 4~10% is added into filtered filtrate, is sufficiently stirred, together
When control enzyme digestion reaction tank in 60~90 DEG C of temperature, pH value be 3~6, decoloration 1~3 hour after recall to pH, filter；
S5, concentration step: the filtrate obtained after filtering is passed through in scraper-type evaporator according to the flow of 800kg/h, and 60
Vacuum concentration processing is carried out at~75 DEG C, obtains 40~50% concentration decoloration hemepeptide feed liquid；
S6, spray drying step: decoloration hemepeptide concentrate is spray-dried, and obtains decoloration hemoglobin powder.
It is disclosed by the invention this for preventing or alleviating the preparation method of the complex polypeptide of anaemia, it has the advantages that
The active peptide obtained by protein digestion, has the function of good alleviation anaemia.In addition pig blood abundance utilizes pig
There is biologically active peptide to be all necessary for blood production.It is to turn the globin in pig blood using pig blood as the biologically active peptide of protein sources
Turn to small-molecular peptides.Small molecule pig blood peptide not only has good dissolubility, low viscosity, anti-gel formative, but also disappears in vivo
Change and absorb fastly, it also has low antigenicity, will not generate allergic reaction and there are the physiological activity such as fat metabolism.It is auxiliary again in hemepeptide
There are the substances such as Radix Angelicae Sinensis, jujube, fructus lycii of function of promoting blood circulation to disperse blood clots with integration of drinking and medicinal herbs, be process complex polypeptide oral solution, compound
Polypeptide agent.This prevention or the complex polypeptide agent of alleviation anaemia, the processing is simple for complex polypeptide oral solution, and mouthfeel is stablized, and effect is bright
Aobvious, no ill-effect is a kind of good functional food.
Hemepeptide in complex polypeptide disclosed by the invention first passes around the polypeptide that hemoglobinase enzymatic hydrolysis is macromolecular, so
Afterwards be added deer placenta powder, improve anaemia medicinal and edible plant digest jointly, make complex polypeptide agent prevent and alleviate anaemia effect
More preferably.It is acted on using the substances small molecule active ingredient such as fructus lycii, jujube, rhizoma polygonati, Radix Angelicae Sinensis enhancing kidney absorption, promoting blood circulation；It utilizes
The complexing free iron ability of hemoglobin and the internal hemoglobin number of increase and activity in deer placenta polypeptide and hemepeptide reinforcement,
It is obvious to improve anaemia effect.
The preparation of 1 hemoglobin of embodiment:
S1, dissolving step: 2kg porcine hemoglobin is poured into enzymatic vessel, and the purified water of 18kg is added, and dissolution is sufficiently stirred.
S2, enzymolysis step: being added the alkali protease of 80g, digests 3 hours, while the temperature in controlled enzymatic hydrolysis reactor tank
60 DEG C, pH value 8.0；
S3, filtration step: enzyme digestion reaction tank material is heated to boiling, and then slightly boiled 10 minutes, after so that protease is lost activity,
Enzyme digestion reaction tank is cooled to 50 DEG C, then uses flame filter press filter material, filter residue is removed, obtains hemepeptide solution；
S4, decolorization process: the active carbon of 160g is added into filtered filtrate, is sufficiently stirred, while controlled enzymatic hydrolysis reactor tank
In 80 DEG C of temperature, pH value 4.8, decoloration 1 hour after recall to pH, filter；
S5, concentration step: the filtrate obtained after filtering is passed through in scraper-type evaporator according to the flow of 800kg/h, and 60
Vacuum concentration processing is carried out at DEG C, obtains 40~50% concentration decoloration hemepeptide feed liquid；
S6, spray drying step: decoloration hemepeptide concentrate is spray-dried, the hemoglobin powder to be decolourized.
Implement the preparation of 2 complex polypeptides:
Radix Angelicae Sinensis 500g, jujube 1500g, fructus lycii 2500g, rhizoma polygonati 1000g is added 1. being on dry matter basis, 22.5kg purifying is added
Water.Under 2000HZ ultrasound condition, ultrasonic 10min.After be cooled to and be cooled to 40 DEG C, be then sonicated 1 time, condition is identical, will
Materials Cell is broken, and cytoplasm dissolution forms plant and is crushed liquid.
2. plant, which is crushed liquid, adds pure water, until solid-to-liquid ratio 1:10,225g cellulase is added to 55 DEG C in constant temperature, digests 3h,
Obtain plant enzymolysis liquid；The plant complex enzyme of 360g is added into plant enzymolysis liquid, digests 7h, keeps constant temperature.Solution is boiled afterwards
5min is boiled, enzyme deactivation is prepared into micromolecular polysaccharide vegetation water.
3. 3760g hemoglobin powder and 740g deer placenta powder is added, adds after the cooling of micromolecular polysaccharide vegetation water
67.5kg purified water prepares compound protein liquid.
4. NaOH is added in compound protein solution, pH9.0 is adjusted, by solution as 60 DEG C of isoperibol, 27g alkali is added
Property protease, maintain 3h.
5. the compound protein liquid that parlkaline is digested adjusts pH7.0, as 50 DEG C of isoperibol, 45g is added
Flavor protease, digest 0.5h, after boil maintain 5min enzyme deactivation, as required micromolecule polypeptide just liquid.
6. liquid at the beginning of micromolecule polypeptide is cooled to 45 DEG C, flame filter press filter material is then used, filter residue is removed, obtains
To the primary filtrate of complex polypeptide.
7. crossing the primary filtrate of complex polypeptide by ultrafiltration membrane, membrane molecule interception amount is 1WDa film, is used after being disposed
1KDa film process, thickening filtration, preparation high concentration, small molecule, the composite polypeptide liquid with certain prevention and alleviation anaemia.It uses
Film filtering can effectively remove macromolecular and small molecule bitter taste amino acid, decoloration.
8. will in composite polypeptide liquid be added 9.9g taurine, 450g fructose syrup, 5.4g peppermint, 960g Momordia grosvenori aglycone,
48g edible essence auxiliary material, in 115 DEG C after filling and sealing, 20Min sterilizing, preparation prevention and the complex polypeptide for alleviating anaemia.
Comparative example 1:
It is added without plant complex enzyme hydrolysis liquid and deer placenta powder, only prepares the polypeptide agent with hemoglobin powder, the specific steps are as follows:
1. purified water is added in hemoglobin powder, until 10 times of dry matter, prepare protein solution.
2. NaOH is added in protein solution, pH9.0 is adjusted, by solution as 60 DEG C of isoperibol, dry matter 0.3% is added
Alkali protease, maintain 3h.
3 protein solutions for digesting parlkaline, adjust pH7.0, and as 50 DEG C of isoperibol, dry matter is added
0.05% flavor protease of (based on compound initial mass), digest 0.5h, after boil maintain 5min enzyme deactivation, it is as required
The first liquid of micromolecule polypeptide.
4. liquid at the beginning of micromolecule polypeptide is cooled to 45 DEG C, flame filter press filter material is then used, filter residue is removed, obtains
To the primary filtrate of polypeptide.
5. crossing the primary filtrate of polypeptide by ultrafiltration membrane, membrane molecule interception amount is 1WDa film, and 1KDa is used after being disposed
Film process, thickening filtration.
6. will in polypeptide liquid be added 0.11g/L taurine, 5g/L fructose syrup, 0.06g/L peppermint, 1% Momordia grosvenori aglycone,
0.5 ‰ edible essence auxiliary materials, in 115 DEG C after filling and sealing, 20Min sterilizing prepares polypeptide agent.
Comparative example 2:
It is directly digested using hemoglobin powder and deer placenta powder, prepares complex polypeptide agent, be added without plant complex enzyme hydrolysis liquid.Specifically
Step:
1. hemoglobin powder and deer placenta powder (in dry weight 5:1 ratio), add purified water, until 10 times of dry matter, it prepares compound
Protein solution.
2. NaOH is added in compound protein solution, pH9.0 is adjusted, by solution as 60 DEG C of isoperibol, dry matter is added
0.3% alkali protease maintains 3h.
The 3 compound protein solution for digesting parlkaline, adjust pH7.0, as 50 DEG C of isoperibol, are added dry
0.05% flavor protease of substance (closing by compound initial mass), digests 0.5h, after boil and maintain 5min enzyme deactivation, as institute
The first liquid of the micromolecule polypeptide needed.
4. liquid at the beginning of micromolecule polypeptide is cooled to 45 DEG C, flame filter press filter material is then used, filter residue is removed, obtains
To the primary filtrate of polypeptide.
5. crossing the primary filtrate of polypeptide by ultrafiltration membrane, membrane molecule interception amount is 1WDa film, and 1KDa is used after being disposed
Film process, thickening filtration.
6. will in polypeptide liquid be added 0.11g/L taurine, 5g/L fructose syrup, 0.06g/L peppermint, 1% Momordia grosvenori aglycone,
0.5 ‰ edible essence auxiliary materials, in 115 DEG C after filling and sealing, 20Min sterilizing prepares pre- polypeptide agent.
Comparative test effect explanation:
By SD rat basal feed and after normal water adaptive feeding three days, modeling rat (A, B, C, D, F) is used instead low
Iron feed is simultaneously aided with deionized water raising, and normal control rat (E) is constant.Modeling rat is generally lower than in content of hemoglobin
(it is judged to anaemia) after 100g/L to start medication.It is administered once a day, each group dosage regimen and dosage are shown in Table shown in 1.Promote
Erythropoietin(EPO) (EPO) is fed, and is the drug-positive control as treatment anaemia.It takes weekly and cuts tail mode and collect blood mark
This, it is anticoagulant using 2.5% EDTA-2Na liquor capacity ratio 1:9, complete blood routine detection.By 1 month nursing period, measurement
The effect of complex polypeptide agent of the invention to improving and treating anaemia.
Wherein low iron feed (iron content 8.4mg/kg is purchased from Beijing Bo Tai hongda Bioisystech Co., Ltd)；Base
Plinth feed (Si Laikang rats and mice breeding material, rise Biotechnology Co., Ltd purchased from Shanghai Pu Lu).
Normal rat erythrocytes number is 5.6 × 1012/ L, content of hemoglobin normal range (NR) 120g/L；Anaemia reference line is
Red blood cell number 4.7 × 1012/ L, content of hemoglobin anaemia range are lower than 120g/L.
Table 1: mouse feeds complex polypeptide situation
Contrast effect result 1:
Number of red blood cells situation comparison such as the following table 2, normal red blood cell of organizing in 1 month blood routine of rat will be fed normally to join
Examine value range >=5.6 × 1012/ L, it is little in normal value fluctuation by its mean constant of red blood cell in one month after feeding chow diet；
Anaemia group (Rat Erythrocytes number≤4.7 × 1012/ L), it feeds low iron feed and is aided with deionized water raising, F group administrable
In the EPO for the treatment of anaemia, the red blood cell of rat rose at the 1st week it is obvious, in recovery in the 2nd week to normal value, after continue hello
It supports, mean constant of red blood cell rises obvious；D group feeds physiological saline and adds low iron feed, and Rat Erythrocytes number declines always, anaemia feelings
Condition is increasingly severe；A group, which is fed, adds low iron feed with complex polypeptide agent of the invention, returns to normal water feeding the 2nd week
Flat, anemia state improves, slightly below drug therapy, hence it is evident that is higher than two groups of B, C.B group, which is fed, adds low iron with the polypeptide agent of comparative example 1
Feed returns to normal level on the 3rd week, and anemia state improves, and effect is lower than A group；C group is fed to be added with the polypeptide agent of comparative example 2
Low iron feed returns to normal level on the 3rd week, and anemia state improves, and effect is lower than A group.
Table 2, the comparison of test effect red blood cell content
Comparative test explanation,
1. complex polypeptide agent disclosed by the invention has treatment similar with drug and alleviation anaemia effect；
2. complex polypeptide agent disclosed by the invention, the medicinal and edible plant of addition digest jointly enhance complex polypeptide alleviation it is poor
The effect of blood；It is mainly reflected in alleviation anaemia effect steadily to rise, closer to drug compared with the polypeptide that integration of drinking and medicinal herbs is not added
Therapeutic effect.
3. complex polypeptide agent disclosed by the invention, the deer placenta of addition digests jointly enhances the alleviation of complex polypeptide agent
The effect of anaemia, is mainly reflected in, rapid-action, alleviates horizontal steady.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the scope of the claims of invention.
Claims (9)
1. a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, it is characterised in that: obtained by following preparation steps
It arrives:
5-15 parts of water of addition after 1 part of a variety of medicinal and edible plant with blood circulation-promoting functions is mixed will be calculated by weight,
2-10min is extracted in ultrasonication under the conditions of 1600-2500HZ, is then cooled to 35-45 DEG C, is carried out ultrasonication again, reach
Clasmatosis, cytoplasm dissolution form plant and are crushed liquid；
It is crushed in liquid to plant and enters water, until solid-to-liquid ratio is 1:10-20, constant temperature is added cellulase and is digested, digested to 55 DEG C
Time is 1-4h, so that plant cell wall digests, obtains plant enzymolysis liquid；
Plant complex enzyme zymohydrolysis 4-10h is added to plant enzymolysis liquid, keeps constant temperature, solution is then boiled into 5min, enzyme deactivation obtains
Micromolecular polysaccharide vegetation water；
Hemoglobin powder, deer placenta powder are added into micromolecular polysaccharide vegetation water, water is then added to 10-20 times of solid, mixes
Uniform compound protein liquid is closed, protease is added into compound protein liquid, is digested as liquid at the beginning of micromolecule polypeptide；
Liquid at the beginning of micromolecule polypeptide is cooled to 45-65 DEG C, liquid at the beginning of filtering micromolecule polypeptide using flame filter press removes filter residue and obtains
To polypeptide refined filtration liquid；
Auxiliary material is added into polypeptide refined filtration liquid, sterilizes after formulated, sterilizing, filling and sealing in 115-121 DEG C, obtains complex polypeptide
Oral solution or Direct spraying are dry, obtain complex polypeptide agent.
2. according to claim 1 a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, feature exists
In: a variety of medicinal and edible plants with blood circulation-promoting functions in step (1) include: 10%-30% Radix Angelicae Sinensis, 30%- according to dry weight calculating
50% jujube, 20%-50% fructus lycii, 10%-30% rhizoma polygonati.
3. according to claim 1 a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, feature exists
In: the weight for the cellulase that step (2) is added is calculated as 2%-7% according to medicinal and edible plant dry weight.
4. according to claim 1 a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, feature exists
In: the weight of the addition plant complex enzyme in step (3) is calculated as 5%-10% according to medicinal and edible plant dry weight.
5. according to claim 1 a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, feature exists
In: the hemoglobin of the addition in step (4) and the weight of deer placenta powder are calculated as medicine food together according to medicinal and edible plant dry weight
1-5 times of source plant weight, wherein the weight ratio of hemoglobin powder and deer placenta powder is 5-10:1.
6. according to claim 5 a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, feature exists
In: the enzymatic hydrolysis in step (4) comprises the following specific steps that: NaOH is added in compound protein liquid, pH to 9.0 is adjusted, solution is set
In 60 DEG C of isoperibol, the alkali protease of dry matter meter 0.1%-0.5% is added, maintains 2-6h, alkali protease will be passed through
The compound protein solution of enzymatic hydrolysis adjusts pH to 7.0, and as 50 DEG C of isoperibol, dry matter meter 0.03%-0.1% flavor is added
Protease, digest 0.5h-1h, after boil maintain 2-5min enzyme deactivation, as required micromolecule polypeptide just liquid.
7. according to claim 1 a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, feature exists
In: the filtering comprises the following specific steps that: the primary filtrate of complex polypeptide being crossed through ultrafiltration membrane, membrane molecule interception amount is 1-5
Ten thousand Da films use 1-2KDa film process after being disposed.
8. according to claim 1 a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, feature exists
In: the auxiliary material is according to polypeptide essence filtrate volume meter, comprising: 0.11g/L taurine, 2-10g/L fructose syrup, 0.05-0.1g/
L peppermint.
9. according to claim 8 a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia, feature exists
In: the auxiliary material is according to polypeptide essence filtrate volume meter, further includes: ‰ edible essence of 0.1%-1% Momordia grosvenori aglycone and 0.1-0.8.
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| CN110934837A (en) * | 2019-12-16 | 2020-03-31 | 西藏天虹科技股份有限责任公司 | Polygonatum sibiricum polypeptide composite tablet and preparation method thereof |
| CN110934837B (en) * | 2019-12-16 | 2021-10-22 | 西藏天虹科技股份有限责任公司 | Polygonatum sibiricum polypeptide composite tablet and preparation method thereof |
| CN113304244A (en) * | 2021-05-26 | 2021-08-27 | 杭州佰倍优生物科技有限公司 | Preparation method of blood protein polypeptide for preventing and relieving anemia |
| CN115462524A (en) * | 2022-08-29 | 2022-12-13 | 杭州佰倍优生物科技有限公司 | A blood peptide with skin caring and antiaging effects, and its preparation method |
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