CN1543987A - Preparing process and application of active peptide-Lutaisu by making use of spotted deer placenta - Google Patents
Preparing process and application of active peptide-Lutaisu by making use of spotted deer placenta Download PDFInfo
- Publication number
- CN1543987A CN1543987A CNA2003101100579A CN200310110057A CN1543987A CN 1543987 A CN1543987 A CN 1543987A CN A2003101100579 A CNA2003101100579 A CN A2003101100579A CN 200310110057 A CN200310110057 A CN 200310110057A CN 1543987 A CN1543987 A CN 1543987A
- Authority
- CN
- China
- Prior art keywords
- embryo cervi
- embryo
- bioactive peptide
- cervi
- mice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention provides a process for preparing deer placenta, a reactive peptide by utilizing sika deer placenta as raw material, preparing dried powder through biological techniques, hydrolyzing at specific conditions of proteolytic enzyme, temperature, acidity, concentration and time, wherein the proteolytic enzyme can be pancreas proteolytic enzyme, oblongas proteolytic enzyme or neutral proteolytic enzyme.
Description
Technical field
The invention belongs to the deep process technology of Flos Mume Embryo cervi, be specifically related to a kind of Flos Mume Embryo cervi that utilizes and prepare bioactive peptide---the method for deer embryo element and the application of this deer embryo element product.
Background technology
In traditional preparation Embryo cervi paste technology, decocting in water boils, and the composition of a lot of biological activitys and drug action causes the great wasting of resources in the destruction Embryo cervi.The patent application of " process of extracting purifying deer's fetus extract " is arranged recently, be to utilize biotechnology from Embryo cervi, to extract the method for purification, and the purpose of this invention is to provide a kind of Flos Mume Embryo cervi that utilizes, prepare the process of biologically active deer embryo element with proteolytic enzyme, both have essential distinction.The present invention makes the biological activity of Embryo cervi and drug action bring into play more fully, and utilization more complete created more large economy benefit.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing enzyme hydrolysis, is the method for feedstock production bioactive peptide with the Flos Mume Embryo cervi, and the application of this product is provided.
The present invention is a raw material with the Flos Mume Embryo cervi, gets fresh or refrigerated Embryo cervi, cleans, shreds, stirs into meat paste, in vacuum drying oven, make dry powder with porcelain dish, and be modulated into the solution that concentration is 2-15% with distilled water at vacuum drying below 80 ℃, temperature is at 45-55 ℃, pH value adds proteolytic enzyme at 6-8, and its amount is the 0.1-2% of Embryo cervi dry powder amount, under agitation, reacted 2-6 hour, during keep pH constant, cessation reaction.Dry powder is made in the reactant liquor lyophilization, also can be made the solution of desired concn through vacuum concentration under 80 ℃.
Description of drawings
Fig. 1: process chart of the present invention.
The specific embodiment
Below we in conjunction with the several embodiment of the present invention, the invention will be further described:
Embodiment 1: with Embryo cervi dry powder, being modulated into concentration with distilled water is 10% solution, 50 ℃ of temperature, under pH value 7.0 conditions, adds 0.5% Trypsin hydrolytic enzyme of Embryo cervi dry powder amount, stirring reaction 4 hours, during keep pH constant, cessation reaction.Dry powder is made in the reactant liquor lyophilization.
Embodiment 2: with Embryo cervi dry powder, being modulated into concentration with distilled water is 10% solution, 45 ℃ of temperature, under pH value 6.7 conditions, adds 0.3% Fructus Chaenomelis proteolytic enzyme of Embryo cervi dry powder amount, stirring reaction 4.5 hours, during keep pH constant, cessation reaction.Dry powder is made in the reactant liquor lyophilization.
Embodiment 3: with Embryo cervi dry powder, being modulated into concentration with distilled water is 3% solution, 50 ℃ of temperature, under pH value 7.0 conditions, adds 0.5% neutral proteolytic enzyme of Embryo cervi dry powder amount, stirring reaction 5 hours, during keep pH constant, cessation reaction.Dry powder is made in the reactant liquor lyophilization.
The pharmacologically active of the Flos Mume Embryo cervi bioactive peptide of the present invention's preparation---deer embryo element detects
Experiment material
1. animal Kunming mouse body weight 11～14g and mice 18～22g.Wistar rat body weight 60～80g, 160～180g and 300～350g.
2. medicine and reagent Embryo cervi dry powder are the Embryo cervi powder of tradition system; The product that Embryo cervi bioactive peptide---deer embryo element makes for the present invention.
Method and result
1, the Embryo cervi bioactive peptide has estrogen-like effects
1.1 to the estrous influence of female immature Mus
60 of female immature Mus are pressed table 1 grouping administration, matched group is pressed 10ml/kg and is irritated the stomach distilled water, irritates stomach every day once, carries out vaginal smear examination simultaneously, observe appearance estrous number of animals (is index the big and random superficial cell of a large amount of shapes to occur), the results are shown in Table 1.The Embryo cervi bioactive peptide can obviously promote the estrous appearance of female immature Mus after administration.
Table 1 Embryo cervi bioactive peptide is to the estrous influence of female immature Mus (x ± s n=12)
The number of mice in rutting period appears behind the group dosed administration
(mg/kg) 8d 9d 10d 11d 12d
Contrast-0 1233
Diethylstilbestrol 428
*9
*11
*12
* *
Embryo cervi bioactive peptide 40 047
*9
*10
*
20 0 4 5 8
* 9
*
Embryo cervi powder 500 03457
*
With compare * P＜0.05, * * P＜0.01, * * * P＜0.001
1.2 to the estrous influence of removal ovary mice
After 80 of adult mices are extractd ovary, raised 20 days, check with the vaginal smear method, select and estrous mice do not occur, press table 2 grouping administration, the Mus number of observation appearance in rutting period the results are shown in Table 2.The Embryo cervi bioactive peptide can obviously promote the estrous appearance of removal ovary mice after administration.
Table 2 Embryo cervi bioactive peptide is to the estrous influence of removal ovary mice (x ± s n=10)
The number of mice in rutting period appears behind the group dosed administration
(mg/kg) 5d 6d 7d 8d 9d
Contrast-0 0011
Diethylstilbestrol 4025 10
* *10
* *
Embryo cervi bioactive peptide 40 00049
*
20 0 0 0 2 7
*
Embryo cervi powder 500 00017
*
With compare * P＜0.05, * * P＜0.01, * * * P＜0.001
1.3 influence to female young rat uterus weight and protein content
50 of rats are pressed table 3 grouping administration, in 15 days, every day gastric infusion once, matched group press 10ml/kg and is irritated the stomach distilled water, behind the last administration 1h, puts to death animal, wins the uterus, weighs, and presses Lowry method survey protein content simultaneously, the results are shown in Table 3.The Embryo cervi bioactive peptide can obviously increase female young rat uterus weight and protein content.
Table 3 Embryo cervi bioactive peptide is to the influence (x ± s n=10) of female young rat uterus weight and protein content
Group dosage (mg/kg) uterus (mg/100g body weight) protein (mg/10g uterus)
Contrast-95 ± 66 3.0 ± 0.6
Diethylstilbestrol 4 160 ± 47
*5.1 ± 0.9
*
Embryo cervi bioactive peptide 40 157 ± 41
*4.2 ± 0.7
*
Embryo cervi peptide 20 150 ± 37
*4.0 ± 0.6
*
Embryo cervi powder 500 144 ± 38
*3.8 ± 0.9
*
By table 1-3 as can be known, the Embryo cervi bioactive peptide has estrogen-like effects, and effect is better than Embryo cervi.2 Embryo cervi bioactive peptide have slow down aging (or skin aging), effects of beautification and nourishing face.
2.1 influence to female eldest child Mus whole blood SOD just and blood plasma MDA
50 of rats are pressed table 4 grouping administration, once a day, continuous 15 days, behind the last administration 1h, get blood, survey superoxide dismutase (SOD) activity with pyrogallol autoxidation method, survey lipid peroxidation product malonaldehyde (MDA) content with the TBA method, the results are shown in Table 4.The Embryo cervi bioactive peptide can obviously strengthen the full SOD activity of female eldest child Mus just, reduces contents of mda.Prompting Embryo cervi bioactive peptide can be used for slow down aging (or skin aging), has effects of beautification and nourishing face.Effect is better than Embryo cervi.
Table 4 Embryo cervi bioactive peptide is to the influence (x ± s n=10) of the female Mus of eldest child just SOD activity and MDA content
Group dosage (mg/kg) SOD (U/ml) MDA (nmol/ml)
Contrast-899 ± 198 7.2 ± 2.0
Embryo cervi peptide 20 1202 ± 221
*3.0 ± 1.1
* *
Embryo cervi bioactive peptide 10 1103 ± 210
*4.5 ± 2.0
*
Embryo cervi powder 500 1002 ± 231
*5.5 ± 2.6
*
3 Embryo cervi bioactive peptide have blood tonification effect
3.1 the Embryo cervi bioactive peptide to the influence of mice iron deficiency anemia with 50 of above-mentioned anemia model mices by the administration of dividing into groups shown in the table, every treated animal male and female half and half, every day gastric infusion once, matched group is irritated stomach distilled water 10ml/kg, after administration the 3rd, 7 day, get blood and survey RBC and Hb value, the results are shown in Table 6 and table 5.In addition, put to death animal, get blood, survey serum levels of iron content, the results are shown in Table 7.
Table 5: the Embryo cervi bioactive peptide to the influence of iron deficiency anemia mice hemoglobin value (x ± s, n=10)
Group dosage Hb (g/dL)
(mg/kg) 0d 3d 7d
Contrast-7.8 ± 0.9 7.9 ± 0.5 12 ± 0.8
Embryo cervi bioactive peptide 40 7.6 ± 0.9 9.2 ± 1.2
*14 ± 1.2
* *
20 7.8±0.9 9.8±1.5
** 14±1.2
***
Embryo cervi powder 500 7.7 ± 0.9 9.0 ± 1.8 13 ± 1.3
*
Compare with matched group
*P＜0.05,
*P＜0.01,
* *P＜0.001, down together
Table 6: the Embryo cervi bioactive peptide to the influence of iron deficiency anemia mouse red blood cell value (x ± s, n=10)
Group dosage RBC (10
12/ L)
(mg/kg) 0d 3d 7d
Contrast-4.5 ± 1.2 5.0 ± 0.4 6.2 ± 0.4
Embryo cervi bioactive peptide 4.0 4.6 ± 0.8 5.9 ± 0.9 6.7 ± 0.4
*
2.0 4.7±0.7 5.9±0.7 6.7±0.5
**
Embryo cervi powder 500 4.7 ± 0.9 5.8 ± 1.5 6.2 ± 1.2
Table 7: the Embryo cervi bioactive peptide to the influence of iron deficiency anemia mice serum iron content (x ± s, n=10)
Group dosage (mg/kg) serum levels of iron (μ g/dL)
Contrast-52 ± 9.3
Embryo cervi bioactive peptide 40 63 ± 8.8
*
20 61±8.4
**
Embryo cervi powder 500 59 ± 8.0
*
By table as seen, the Embryo cervi bioactive peptide can obviously increase iron deficiency anemia mice Hb content on the 3rd, 7 day after administration; Can obviously increase iron deficiency anemia mice RBC quantity; Can obviously increase iron deficiency anemia mice serum iron content.
This experimentation shows that the Embryo cervi bioactive peptide has blood tonification effect to the iron deficiency anemia mice.Effect is better than Embryo cervi
4. the Embryo cervi bioactive peptide has antiinflammatory action
4.1 the influence that mice dimethylbenzene is caused mice ear with 50 of adult mices by table grouping administration, in 7 days, irritate stomach every day once, matched group is irritated stomach equal-volume distilled water, 30min after the last administration, dimethylbenzene is dripped in mouse right ear, behind the 15min, put to death animal, punch in left and right sides ear same area with the 6mm card punch, as the swelling level index, the results are shown in Table 8 with two auricle weight differences.Embryo cervi bioactive peptide xylol causes that mice ear has the obvious suppression effect.
Table 8: the Embryo cervi bioactive peptide to mice dimethylbenzene cause mice ear influence (x ± s, n=10)
Group dosage (mg/kg) two auricle weight differences (mg) press down swollen rate (%)
Contrast-8.2 ± 3.1
Embryo cervi bioactive peptide 40 5.0 ± 2.0
*46
20 5.6±3.0
* 39
Embryo cervi powder 500 5.8 ± 1.5
*40
With compare,
*P＜0.05
5. the Embryo cervi bioactive peptide has analgesic activity
5.1 the influence to the mice hot plate threshold of pain time in 7 days, is irritated stomach every day once by table grouping administration with 50 of adult mices, matched group is irritated stomach equal-volume distilled water, behind the last administration 1h, measures the mice threshold of pain time, the results are shown in Table 9.The Embryo cervi bioactive peptide can obviously prolong the mice hot plate threshold of pain time.
Table 9: the Embryo cervi bioactive peptide to the influence of threshold of pain time of mice hot plate (x ± s, n=10)
Group dosage (mg/kg) threshold of pain time (min)
Contrast-19 ± 4.9
Embryo cervi bioactive peptide 40 40 ± 19
*
20 30±18
*
Embryo cervi powder 500 29 ± 15
*
With compare,
*P＜0.01
5.2 the influence that Dichlorodiphenyl Acetate causes mouse writhing reaction with 50 of adult mices by table grouping administration, in 7 days, irritate stomach every day once, matched group is irritated stomach equal-volume distilled water, behind the last administration 1h, whole mouse peritoneals are injected 0.6% acetum, and the number of times of writhing response takes place in the record mice in 10min, the results are shown in Table 10.The Embryo cervi bioactive peptide can obviously reduce the number of times that acetic acid causes the mouse writhing reaction.
Table 10: Embryo cervi bioactive peptide Dichlorodiphenyl Acetate causes the influence (x ± s) of mouse writhing reaction
Group dosage (mg/kg) writhing response number of times (inferior)
Contrast-35 ± 3.4
Embryo cervi bioactive peptide 40 11 ± 3.9
* *
20 13±4.0
***
Embryo cervi powder 500 14 ± 4.6
*
With compare,
* *P＜0.001
6. the Embryo cervi bioactive peptide has immunological enhancement
6.1 the influence to mouse monokaryon macrophage phagocytic ability is pressed table grouping administration with 50 of adult mices, in 7 days, irritate stomach every day once, matched group is irritated stomach equal-volume distilled water, behind the last administration 1h, with whole mouse tail vein injection prepared Chinese ink (1: 4) 0.1ml/ only, respectively at injection back 2min and 5min, eye socket is got blood 10 μ l, adds 0.1%Na
2CO
3Among the solution 2ml,, behind sacrifice of animal, get liver and spleen, weigh, calculate phagocytic index K value and α value, the results are shown in Table 11 at 600nm place photometry density value.The Embryo cervi bioactive peptide can obviously increase the phagocytic index K value and the α value of mouse monokaryon macrophage.
Table 11: the Embryo cervi bioactive peptide to the influence of mouse monokaryon macrophage phagocytic ability (x ± s, n=10)
Group dosage (mg/kg) phagocytic index K value phagocytic index α value
Contrast-0.020 ± 0.002 5.0 ± 0.35
Embryo cervi bioactive peptide 40 0.029 ± 0.005
*5.7 ± 0.40
*
20 0.027±0.004
* 5.6±0.60
*
Embryo cervi powder 500 0.027 ± 0.005
*5.5 ± 0.6
*
With compare,
*P＜0.05
*P＜0.01
7. the Embryo cervi bioactive peptide has the sleep of enhancing effect
7.1 press table grouping administration with 50 of adult mices the long length of one's sleep, in 7 days, irritates stomach every day once, matched group is irritated stomach equal-volume distilled water, behind the last administration 1h, with whole ip in mice pentobarbital sodium 55mg/kg, under 24～25 ℃ of environment, the record mouse sleep time the results are shown in Table 12.The Embryo cervi bioactive peptide can obviously prolong mice pentobarbital sodium length of one's sleep.
Table 12: the Embryo cervi bioactive peptide to the influence of the length of one's sleep of mice pentobarbital sodium (x ± s, n=10)
Group dosage (mg/kg) length of one's sleep (min)
Contrast-41 ± 21
Embryo cervi bioactive peptide 40 60 ± 11
*
20 58±14
*
Embryo cervi powder 500 56 ± 13
*
With compare,
*P＜0.05
7.2 syngignoscism is pressed table grouping administration with 50 of adult mices, in 7 days, irritate stomach every day once, matched group is irritated stomach equal-volume distilled water, behind the last administration 1h, with whole ip in mice pentobarbital sodium 30mg/kg, under 24～25 ℃ of environment, occur the number of animals (to be index more than the righting reflex loss 1min) of sleep in the record 15min, the results are shown in Table 13.The Embryo cervi bioactive peptide can obviously increase the number of mice that sleep appears in the sub-threshold dose pentobarbital sodium.
Table 13: the Embryo cervi bioactive peptide is to the influence of mice sub-threshold dose pentobarbital sodium effect (x ± s)
Sleep number of animals (only) occurrence rate (%) appears in group dosage (mg/kg) number of animals (only)
Contrast-10 2 20
Embryo cervi bioactive peptide 40 10 8 80
*
20 10 7 70
*
Embryo cervi powder 500 10 7 70
*
With compare,
*P＜0.05
8. the Embryo cervi bioactive peptide has anticonvulsant action
8.1 the influence that strychnine is caused mice convulsion with 50 of adult mices by table grouping administration, in 7 days, irritate stomach every day once, matched group is irritated stomach equal-volume distilled water, behind the last administration 1h, with whole ip in mice strychnine 1.5mg/kg, record mice convulsion generation number, time of occurrence (time from the injection strychnine to the tonic convulsion appearance), death time (time of taking place from the tonic convulsion to death), mortality rate the results are shown in Table 14.The Embryo cervi bioactive peptide can obviously prolong strychnine and cause mice convulsion time of occurrence and death time, and mice convulsion generation number and mortality rate are not had obvious influence.
Table 14: the Embryo cervi bioactive peptide causes the influence (x ± s) of mice convulsion to strychnine
Group dosage number of animals occurs fainting from fear the death time death toll occurring
(mg/kg) (only) number of animals (only) time (sec) (sec) (only)
Contrast-10 10 148 ± 19 31 ± 18 10
Embryo cervi bioactive peptide 40 10 10 198 ± 68
*85 ± 64
*10
20 10 10 162±35 71±22
* 10
Embryo cervi powder 500 10 10 160 ± 36 70 ± 23
*10
With compare,
*P＜0.05
8.2 the influence that caffeine and sodium benzoate is caused mice convulsion is with 50 groupings of adult mice administration, behind the last administration 1h, with whole mouse subcutaneous injection caffeine and sodium benzoate 800mg/kg, observation index is the same, the results are shown in Table 15.The Embryo cervi bioactive peptide can obviously prolong caffeine and sodium benzoate and cause the mice convulsion time of occurrence, and mouse diing time, convulsions generation number and mortality rate are not had obvious influence.
Table 15: the Embryo cervi bioactive peptide causes the influence (x ± s) of mice convulsion to caffeine and sodium benzoate
Group dosage number of animals occurs fainting from fear the death time death toll occurring
(mg/kg) (only) number of animals (only) time (sec) (sec) (only)
Contrast-10 10 312 ± 102 586 ± 301 10
Embryo cervi bioactive peptide 40 10 10 413 ± 103
*645 ± 223 10
20 10 10 395±84
* 674±235 10
Embryo cervi powder 500 10 10 397 ± 82
*652 ± 213 10
With compare,
*P＜0.05
Above-mentioned experimentation proves, the Embryo cervi bioactive peptide can obviously prolong mice pentobarbital sodium length of one's sleep, increase the number of mice that sleep appears in the sub-threshold dose pentobarbital sodium, the prolongation strychnine causes mice convulsion time of occurrence and death time and prolongs caffeine and sodium benzoate and causes the mice convulsion time of occurrence.Show that the Embryo cervi bioactive peptide has certain hypnosis, calmness, anticonvulsant action.
9. the Embryo cervi bioactive peptide has oxygen lack resistant function
9.1 the influence to mice normal pressure hypoxia-bearing capability is pressed table grouping administration with 50 of adult mices, in 7 days, irritate stomach every day once, matched group is irritated stomach equal-volume distilled water, and behind the last administration 24h, it is the wide mouthed bottle that 250ml contains the 5g sodica calx that mice is put into volume, jam-pack, 1/bottle, the record mice time-to-live, the results are shown in Table 16.The Embryo cervi bioactive peptide can obviously prolong the time-to-live under the mice normobaric hypoxia.
Table 16: the Embryo cervi bioactive peptide to the influence of mice normal pressure hypoxia-bearing capability (x ± s, n=10)
Group dosage (mg/kg) time-to-live (min)
Contrast-32 ± 8.9
Embryo cervi bioactive peptide 40 45 ± 13
*
20 43±11
*
Embryo cervi powder 500 42 ± 13
*
With compare,
*P＜0.05
10 Embryo cervi bioactive peptide have antifatigue effect (strong effect)
10.1 the influence to the mice swimming time is pressed table grouping administration with 50 of adult mices, in 7 days, irritates stomach every day once, matched group is irritated stomach equal-volume distilled water, and behind the last administration 24h, it is in 23～25 ℃ the water that mice is dropped into temperature, record mice swimming time the results are shown in Table 17.The Embryo cervi bioactive peptide can obviously prolong the mice swimming time.
Table 17: the Embryo cervi bioactive peptide to the influence of mice swimming time (x ± s, n=10)
Group dosage (mg/kg) swimming time (min)
Contrast-196 ± 36
Embryo cervi bioactive peptide 40 285 ± 58
* *
20 265±53
*
Embryo cervi powder 500 259 ± 48
*
With compare,
*P＜0.05,
* *P＜0.001
11. the Embryo cervi bioactive peptide has the promotion protein synthesis
11.1 the influence to murine liver tissue DNA, RNA and protein content is pressed table grouping administration with 50 of adult mices, in 7 days, irritate stomach every day once, matched group is irritated stomach equal-volume distilled water, behind the last administration 1h, mice is put to death, get hepatic tissue, survey DNA, RNA and protein content, the results are shown in Table 18.The Embryo cervi bioactive peptide can obviously increase murine liver tissue RNA and protein content, and dna content is also had the increase effect, but no difference of science of statistics.
Table 18: the Embryo cervi bioactive peptide to the influence of mice hepatic DNA, RNA and protein content (x ± s, n=10)
Group dosage (mg/kg) DNA (mg/g) RNA (mg/g) protein (mg/g)
Contrast-3.6 ± 0.2 14 ± 1.1 252 ± 28
Embryo cervi bioactive peptide 40 5.0 ± 0.6 18 ± 1.0
*287 ± 25
*
20 4.7±0.4 17±1.2
* 276±19
*
Embryo cervi powder 500 4.6 ± 0.4 16 ± 1.1
*270 ± 16
*
With compare
*P＜0.05
This experimentation shows that the Embryo cervi bioactive peptide has certain anoxia enduring and antifatigue effect.The Biochemical Mechanism of its effect is relevant with protein content with its increase hepatic tissue RNA probably.
Through above-mentioned to the Embryo cervi bioactive peptide---the pharmacologically active of deer embryo element detects as can be seen, has very significantly advantage through the prepared deer embryo element of the present invention than former Embryo cervi dry powder.Can be applicable in the biochemical drug, both can make folk prescription, also can make compound recipe and use.Have enrich blood, pharmacodynamic feature such as antiinflammatory, analgesia, enhance immunity activity and estrogen-like effects.
Aspect cosmetics, use the skin protection cosmetics that prepared deer embryo element lyophilized powder of the present invention and concentrated solution are prepared, have the effect that makes easily absorption of skin, skin moistening, skin care, beauty treatment, delaying decrepitude of skin.
Claims (4)
1, a kind of Embryo cervi bioactive peptide for preparing---the method for deer embryo element, the steps include: to get fresh or refrigerated Embryo cervi, clean, shred, stir into meat paste, with porcelain dish in vacuum drying oven, make dry powder at vacuum drying below 80 ℃, and be modulated into the solution that concentration is 2-15% with distilled water, and temperature is at 45-55 ℃, and pH value is at 6-8, add proteolytic enzyme, its amount is the 0.1-2% of Embryo cervi dry powder amount, under agitation, reacts 2-6 hour, keep pH constant during this time, after the cessation reaction dry powder is made in the reactant liquor lyophilization, also can be made the solution of desired concn through vacuum concentration under 80 ℃.
2, the Embryo cervi bioactive peptide for preparing as claimed in claim 1---the method for deer embryo element is characterized in that: proteolytic enzyme can be Trypsin hydrolytic enzyme, Fructus Chaenomelis proteolytic enzyme or neutral proteolytic enzyme.
3, the deer embryo element of claim 1 or 2 methods preparation, be used for preparing have enrich blood, the application of the medicine of antiinflammatory, analgesia, enhance immunity activity and estrogen-like effects.
4, the deer embryo element of claim 1 or 2 methods preparation is in the application that is used for preparing the cosmetics with skin moistening, skin care, beauty treatment, delaying decrepitude of skin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101100579A CN1251690C (en) | 2003-11-18 | 2003-11-18 | Preparing process and application of active peptide-Lutaisu by making use of spotted deer placenta |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101100579A CN1251690C (en) | 2003-11-18 | 2003-11-18 | Preparing process and application of active peptide-Lutaisu by making use of spotted deer placenta |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1543987A true CN1543987A (en) | 2004-11-10 |
| CN1251690C CN1251690C (en) | 2006-04-19 |
Family
ID=34335493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101100579A Expired - Fee Related CN1251690C (en) | 2003-11-18 | 2003-11-18 | Preparing process and application of active peptide-Lutaisu by making use of spotted deer placenta |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1251690C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100571542C (en) * | 2006-11-29 | 2009-12-23 | 山东师范大学 | The preparation method of venison enzymolysis oral liquid |
| CN102669669A (en) * | 2012-05-30 | 2012-09-19 | 吉林大学 | Deer spleen extract preparation and preparation method thereof |
| CN102872266A (en) * | 2012-10-09 | 2013-01-16 | 吉林大学 | Fetus cervi compound medicinal preparation and method for preparing same |
| CN102925523A (en) * | 2012-11-15 | 2013-02-13 | 辽宁大学 | Enzyme hydrolysis method for preparing fetus cervi active peptide |
| CN102919863A (en) * | 2012-10-31 | 2013-02-13 | 吉林大学 | Sika deer liver/heart powder or particle and preparation method and application thereof |
| CN109793885A (en) * | 2019-03-13 | 2019-05-24 | 湖北瑞邦生物科技有限公司 | It is a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia |
| CN110812310A (en) * | 2019-11-14 | 2020-02-21 | 融致丰生制药有限公司 | Deer fetus extract and composition for preventing alopecia, nourishing and growing hair, and preparation method and application thereof |
-
2003
- 2003-11-18 CN CNB2003101100579A patent/CN1251690C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100571542C (en) * | 2006-11-29 | 2009-12-23 | 山东师范大学 | The preparation method of venison enzymolysis oral liquid |
| CN102669669A (en) * | 2012-05-30 | 2012-09-19 | 吉林大学 | Deer spleen extract preparation and preparation method thereof |
| CN102669669B (en) * | 2012-05-30 | 2013-09-18 | 吉林大学 | Deer spleen extract preparation and preparation method thereof |
| CN102872266A (en) * | 2012-10-09 | 2013-01-16 | 吉林大学 | Fetus cervi compound medicinal preparation and method for preparing same |
| CN102872266B (en) * | 2012-10-09 | 2015-01-14 | 吉林大学 | Fetus cervi compound medicinal preparation and method for preparing same |
| CN102919863A (en) * | 2012-10-31 | 2013-02-13 | 吉林大学 | Sika deer liver/heart powder or particle and preparation method and application thereof |
| CN102919863B (en) * | 2012-10-31 | 2013-12-04 | 吉林大学 | Sika deer liver/heart powder or particle and preparation method and application thereof |
| CN102925523A (en) * | 2012-11-15 | 2013-02-13 | 辽宁大学 | Enzyme hydrolysis method for preparing fetus cervi active peptide |
| CN102925523B (en) * | 2012-11-15 | 2014-09-10 | 辽宁大学 | Enzyme hydrolysis method for preparing fetus cervi active peptide |
| CN109793885A (en) * | 2019-03-13 | 2019-05-24 | 湖北瑞邦生物科技有限公司 | It is a kind of for preventing or alleviating the preparation method of the complex polypeptide of anaemia |
| CN109793885B (en) * | 2019-03-13 | 2022-07-08 | 湖北瑞邦生物科技有限公司 | Preparation method of composite polypeptide for preventing or relieving anemia |
| CN110812310A (en) * | 2019-11-14 | 2020-02-21 | 融致丰生制药有限公司 | Deer fetus extract and composition for preventing alopecia, nourishing and growing hair, and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1251690C (en) | 2006-04-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1390594A (en) | Mixture for improving blood circulation | |
| CN1799429A (en) | Beverage with bamboo and siberian solomoseal rhizome its preparation method | |
| CN1543987A (en) | Preparing process and application of active peptide-Lutaisu by making use of spotted deer placenta | |
| CN1920049A (en) | Small molecule bioactive peptide, preparation method, composition and application thereof | |
| CN1799561A (en) | Application of Antrodia camphorata mycelium fermented extract in preparation of anti-radiation damage medicine | |
| CN1872278A (en) | A composition of medication | |
| CN1167459C (en) | Nutritive health food with functions of reducing blood fat and delaying senility and its prepn process | |
| CN1883527A (en) | A health product of lucid ganoderma and preparation method thereof | |
| CN101036642A (en) | Ginkgolide B nanometric liposomes medicine and the preparing method thereof | |
| CN1067538C (en) | Health-care powder | |
| CN1869208A (en) | Preparation of caterpillar fungus SOD and health-care product containing caterpillar fungus SOD | |
| CN1831015A (en) | Process for extracting fructus ligustri lucidi and Radixa stragali polysaccharide, the products and application thereof | |
| CN1172926C (en) | Astragalus calycosin and its glycoside with the function of resisting myocardial ischemia | |
| CN1166402C (en) | Composite Chinese medicine with health care function | |
| CN1651036A (en) | Angelica root mistletoe granules and its preparation method | |
| CN1099866C (en) | Natural spiroalgae cosmetic for skin and hair cleaning and protection | |
| CN1335161A (en) | Deer bone powder capsule | |
| CN1569054A (en) | Dripping pills of 'Xiao Chaihu' | |
| CN1733209A (en) | Injection for restoring pulse beat and process for preparing the same | |
| CN1907254A (en) | Cosmetic kreotoxin paste | |
| CN1193758C (en) | Preparation of theophylline or aminophylline supplied through skin for treating respiratory disease as well as its preparing method | |
| CN1490050A (en) | Hepatitis A inactivated vaccine | |
| CN1561960A (en) | Method for preparing biological beauty skin-care product containing keratin cell growth factor-2 and recombined human keratin cell growth factor-2 | |
| CN1104120A (en) | Senile-resistant and blood-fat reducing medicinal liquor made from Chinese traditional medicine | |
| CN1565438A (en) | Application of 5-hydroxymethylfurfural in preparation of medicines for nervous system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |