CN1350860A - Process for preparing health-care oral liquid for diabetics - Google Patents

Process for preparing health-care oral liquid for diabetics Download PDF

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Publication number
CN1350860A
CN1350860A CN 00131110 CN00131110A CN1350860A CN 1350860 A CN1350860 A CN 1350860A CN 00131110 CN00131110 CN 00131110 CN 00131110 A CN00131110 A CN 00131110A CN 1350860 A CN1350860 A CN 1350860A
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China
Prior art keywords
cordyceps
liquid
water
radix
fermentation
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CN 00131110
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Chinese (zh)
Inventor
陈萍
郭跃荣
王震宇
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851 ORAL LIQUOR FACTORY INST OF BIOENGINEERING FUJIAN AGRICULTURAL UNIV
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851 ORAL LIQUOR FACTORY INST OF BIOENGINEERING FUJIAN AGRICULTURAL UNIV
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Priority to CN 00131110 priority Critical patent/CN1350860A/en
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Abstract

A health-care oral liquid for diabetics is prepared from 10 Chiense-medicinal materials including fermented liquid of cordyceps, wolfberry fruit, yam, fleece flower root, etc. It features rich nutrients such as amino acids, trace elements and isoflavone, and low cost.

Description

The process for preparing health-care oral liquid that the diabetes patient uses
The present invention relates to the process for preparing health-care oral liquid that a kind of diabetes patient uses.
Diabetes are a kind of common endocrine metabolism diseases, can cause heart. kidney. and eye. peripheral vessels. many organs of whole body multisystem chronic complicating diseases such as nerve.Prevalence is about 6% in developed country, has become to be only second to malignant tumor and cardiovascular disease the third-largest disease afterwards.At present, though various medicines application of treatment diabetes are clinically arranged, how incomplete, as producing problems such as drug resistance and side effect.Number of patent application is that 95109071 patent application discloses a kind of " production process of amino acid oral liquid for diabetic ", this oral liquid is formed by the pure mother solution that contains 20 seed amino acids that utilizes Radix Ginseng to extract and certain Chinese herbal medicine decoction's liquid and flavoring agent modulation, its purpose is to solve the diabetes patient because of limit food. prohibit sugar. and diet nutritional deficiency, lack amino acid and cause sick body to recover to wait slowly problem.Yet, since this oral liquid with Radix Ginseng as primary raw material, so cost is higher, be unfavorable for applying.
The process for preparing health-care oral liquid that will provide a kind of diabetes patient to use is provided, the product that this production technology makes not only is rich in aminoacid and to human body beneficial's various trace elements, and isoflavone content is higher, and production cost is lower, is of value to diabetes patient.
Production technology of the present invention is characterised in that:
(1) prescription (weight proportion): Cordyceps mycelium fermented liquid 45~55%, structure Qi 5~10%, Rhizoma Dioscoreae 3~5%, Radix Polygoni Multiflori 5~10%, Radix Codonopsis 3~5%, the Radix Astragali 3~5%, Radix Glycyrrhizae 3~5%, Radix Pseudostellariae 3~5%, Radix Ginseng 2~5%, Lac regis apis 1~3%.
(2) production craft step is:
1. Cordyceps mycelium fermented liquid technological process:
1.1 the Cordyceps strain expanding species is cultivated.
1.2 shake-flask seed is cultivated.
1.3 fermentor cultivation
1.4 after the Cordyceps mycelium fragmentation, add the compound enzyme system of protein pectase cellulase formation through enzymolysis to fermentation culture; Enzyme denaturing; Add water, water is carried; Solid-liquid separation; At last enzymolysis solution is concentrated, make Cordyceps mycelium fermented liquid.
2. Chinese medicine processing. decoct:
2.1 the Radix Ginseng in will filling a prescription is pulverized, and adds the ethanol that concentration is 50-75%, water-bath refluxes, and puts cold after-filtration, keeps filtrate.Behind the filtrate recycling ethanol, be condensed into syrupy shape, standby.
2.2 other herbal raw material pulverize separately becomes granule in will filling a prescription, and sneaks into the ginseng residue and drops in the extraction pot, adds water, soaks, and decocts, and filters, and extracts filtrate for later use.
3. allotment. concentrate:
With Chinese medicine extraction liquid, the Cordyceps mycelium fermented liquid mix homogeneously is concentrated into soluble solid and all reaches 6-8% (20C refractometer mensuration), mixes Lac regis apis by formula proportion, fully stirs, and filters, and accent PH is 5.0-6.0.
The prescription composition of health care oral liquid of the present invention is mainly considered from nourishing YIN and clearing away heat. the kidney invigorating. and invigorating the spleen and benefiting QI. blood circulation promoting and blood stasis dispelling. dispersing the stagnated live-QI to relieve the stagnation of QI. supplementing QI and nourishing YIN, the promptly dirty integrally-regulated balance of negative and positive gas of bowing is started with.Cordyceps wherein. Radix Ginseng. Radix Codonopsis. Radix Pseudostellariae. Fructus Lycii. Radix Polygoni Multiflori. the Radix Rehmanniae. Rhizoma Dioscoreae. raw materials such as Lac regis apis are rich in isoflavone. protein. the polypeptide class. the ginsenoside. biological glycoside. active skull cap components such as guanidine derivatives, not only has good hypoglycemic activity, (its mechanism of action mainly is by promoting the islet cells excreting insulin, promote the islet cells reparation. regeneration, promote peripheral tissues's cell to the utilization of Fructus Vitis viniferae to suppress of the picked-up of intestinal wall cell to glucose, increase the content of hepatic glycogen, the effect by two-ways regulation reaches good hypoglycemic effect); But also have blood fat reducing. blood pressure lowering. prevention of arterial sclerosis prevention of arterial is atherosis. prophylaxis of tumours. suppress activity of tumor cells. antifungal. antioxidation. human body immunity improving power. promote rehabilitation. effects such as health invigorating.Therefore invention helps the generation of early stage active prevention control of diabetes and complication thereof, to reduce and to avoid the serious harm of diabetes to the mankind.
In addition, the prescription main body be the culture medium and the Chinese herbal medicine of cheapness, in the prescription proportioning of Radix Ginseng seldom, so production cost is lower.
Below in conjunction with embodiment the present invention is specifically described.
The production technology of embodiment is characterised in that:
(1) composition of raw materials (weight proportion) is: Cordyceps mycelium fermented liquid 45~55% (getting 50%), structure Qi 5~10% (getting 7%), Rhizoma Dioscoreae 3~5% (getting 4%), Radix Polygoni Multiflori 5~10% (getting 7%), Radix Codonopsis 3~5% (getting 4%), the Radix Astragali 3~5% (getting 4%), Radix Glycyrrhizae 3~5% (getting 4%), Radix Pseudostellariae 3~5% (getting 4%), Radix Ginseng 2~5% (getting 3%), Lac regis apis 1~3% (getting 2%).
(2) production craft step is:
1. the grass mycelium submerged fermentation of worm summer is cultivated
1.1 culture medium preparation:
(a) expanding species is cultivated: the female culture medium of test tube: PDA culture medium
(b) shake-flask seed culture medium: Semen Glycines powder 2-8% (getting 5%), sucrose 1-5% (getting 1.5%), potassium dihydrogen phosphate 0.1-1% (getting 0.5%), the beautiful 0.05-0.5% (getting 0.3%) of sulphuric acid.
(c) fermentation tank culture medium: Semen Glycines powder 5-12% (getting 8%), sucrose 1-5% (getting 3%), potassium dihydrogen phosphate 0.1-1% (getting 0.5%), the beautiful 0.05-0.5% (getting 0.3%) of sulphuric acid, pH nature
------1.2 shake-flask seed cultivation---fermentor cultivation---fermentation stops strain deep layer fermenting process flow process: the Cordyceps preservation strain of directive breeding
1.3 key points for operation:
1.3.1 cultivating, shake-flask seed adopts rotary shaking table, 100 times/min of shaking table frequency, inoculum concentration 10-15% (getting 13%), through 3-4 days (getting 3 days), after 25-27C (the getting 26 ℃) constant temperature culture, it is pale yellow little turbid to ferment, and forms the mycelium pellet of the about 0.2-0.5cm size of diameter, and fermentation liquid has a kind of inherent delicate fragrance to shake flavour of a drug.
1.3.2 temperature:
When Cordyceps submerged fermentation cultivation optimum temperature was 25-27 ℃ (getting 26 ℃), when temperature was lower than 25C, mycelial growth obviously was suppressed: be higher than 28 ℃ of easy old and feeble self-dissolvings of mycelial growth, culture fluid darkened.
1.3.3.:
The Cordyceps deep layer is cultivated and is required to have a meta-acid environment, and this has often considered when selecting culture medium constituent, has not needed during preparation to test pH value specially, and give free rein to.
1.3.4 ventilation:
Ventilation is cultivated very important to the Cordyceps deep layer, general ventilation is with 1: 0.5-1: 1.0/min (gets 1: 0.8/min) be advisable.Bubbling air is too much or very few, all is unfavorable for mycelial growth.
1.3.5
The ventilation deep layer is cultivated jar standard of putting: hyphae content " 15% (weight in wet base); Abnormal smells from the patient delicate fragrance; Residual total sugar " 1.2%; Ammoniacal nitrogen<30mg/m); Fermentation liquid is thickness very, shape such as paste; The mycelia aging, minority self-dissolving, cultivation cycle 3-4 days (getting 3 days).
1.3.6 biological processes:
The mycelium process is broken, add protein. pectase. the compound enzyme that cellulase constitutes is 1%, 55 ℃ of enzymolysis 60 minutes, 95-100 ℃ (getting 98 ℃), 3-5min (getting 4min) enzyme denaturing adds 5-10 times of water (getting 7 times), and 90-100 ℃ of (getting 80 ℃) water is carried, hour 1.5-2 (getting 2 hours), high speed centrifugation carries out solid-liquid separation.Residue is handled with homogenizer, added 3-5 times of water (getting 4 times), 90-100 ℃ (getting 90 ℃) extracts 1-1.5 hour (getting 1 hour), and high speed centrifugation separates, and clear liquid and enzymolysis solution merge, and molten the contracting of reducing pressure obtains the Cordyceps extracting solution.
2. Chinese medicine processing. decoct:
2.1 Radix Ginseng is pulverized, adding 5-8, doubly to measure (getting 6 times) concentration be 50-75% (getting 70%) ethanol, water-bath refluxed 2 hours, put the double-deck filter paper filtering in cold back, kept filtrate, medicinal residues are same to be extracted 4 times, merge 5 times alcohol extract, the double-deck filter paper of reuse and to add Pulvis Talci be that filter aid filters is behind the filtrate recycling ethanol, be condensed into syrupy shape, standby.
2.2 its body herbal raw material pulverize separately becomes diameter 2.0-3.0 mm granules in will filling a prescription, sneak into the ginseng residue and drop in the extraction pot, add 5-10 times of water (getting 8 times), soak 2-3h (getting 2h) by formulation ratio, then in 90-100 ℃ (getting 90 ℃), decoct 60--90min (getting 70min), filter, filtering residue adds 3-5 times of water (getting 3 times), get 90 ℃ in 90-100 ℃ 9 again), decoct 30min, filter merging filtrate.
3. allotment. concentrate:
With Chinese medicine extraction liquid, Chinese caterpillar fungus fermentation extracting solution mix homogeneously injects the cryogenic vacuum concentration tank and concentrates.Cycles of concentration is 1.5-2.5 times (getting 1.5 times) of stock solution, and soluble solid all reaches 6-8% (getting 6%) (20 ℃ of refractometers are measured).Mix Lac regis apis in proportion, fully stir, filter.Accent PH is 5.0-6.0.
4. fill:
Above concentrated solution is used 250ml brown bottle filling and capping respectively, and 120 ℃, the 45min sterilization, after the assay was approved, i.e. finished product.
Oral liquid of the present invention after testing is rich in 17 kinds with upper amino acid, macroelement calcium. magnesium. and phosphorus, trace element-selenium. zinc. the road, vitamin B1 .B2.C and biological activity Specific Factors for Health, as: isoflavone. nucleic acid. polypeptide. polysaccharide. mannitol. Saponin.Amino acid content: 6260mg/l wherein; Isoflavone: 440.1 μ g/ml; Nucleic acid 0.5mg/ml; Mannitol 6.17mg/ml; Saponin 349mg/l.Test shows that this oral liquid is that young Mus can promote growth, physical strength reinforcing heart and endurance anoxia ability significantly to underfed ICR.To the mice of adrenal gland's disposition hyperglycemia and high sugar diabetes model, press 10g.kg respectively with oral liquid of the present invention -1And 30g.kg -1Body weight dosage, continuous irrigation stomach 3 days, the result shows that all it has significant inhibition and the effect of hyperglycemia drug effect to two kinds of dosage.Compared with similar products, " Ecogen-851 " isoflavone-containing is 98 μ g/ml; " BESO-851 " isoflavone-containing is 81 μ g/ml, and it is 68.9 μ g/ml that Beijing three essentials-essence is revealed isoflavone-containing, and the natural isoflavone content of oral liquid of the present invention is 440.1ug/ml, for the highest in the like product.

Claims (2)

1. the process for preparing health-care oral liquid used of a diabetes patient is characterized in that:
(1) prescription (weight proportion): Cordyceps mycelium fermented liquid 45~55%, structure Qi 5~10%, Rhizoma Dioscoreae 3~5%, Radix Polygoni Multiflori 5~10%, Radix Codonopsis 3~5%, the Radix Astragali 3~5%, Radix Glycyrrhizae 3~5%, Radix Pseudostellariae 3~5%, Radix Ginseng 2~5%, Lac regis apis 1~3%.
(2) production craft step is:
(1) Cordyceps mycelium fermented liquid technological process:
1.1 the Cordyceps strain expanding species is cultivated.
1.2 shake-flask seed is cultivated.
1.3 fermentor cultivation
1.4 after the Cordyceps mycelium fragmentation, add the compound enzyme system of protein pectase cellulase formation through enzymolysis to fermentation culture; Enzyme denaturing; Add water, water is carried: solid-liquid separation; At last enzymolysis solution is concentrated, make Cordyceps mycelium fermented liquid.
(2) Chinese medicine processing decocts:
2.1 the Radix Ginseng in will filling a prescription is pulverized, and adds the ethanol that concentration is 50-75%, water-bath refluxes, and puts cold after-filtration, keeps filtrate, behind the filtrate recycling ethanol, is condensed into syrupy shape, and is standby.
2.2 other herbal raw material pulverize separately becomes granule in will filling a prescription, and sneaks into the ginseng residue and drops in the extraction pot, adds water, soaks, and decocts, and filters, and extracts filtrate for later use.
(3.) allotment concentrates:
With Chinese medicine extraction liquid, the Cordyceps mycelium fermented liquid mix homogeneously is concentrated into soluble solid and all reaches 6-8% (20 ℃ of refractometers are measured), mixes Lac regis apis by formula proportion, fully stirs, and filters, and accent PH is 5.0-6.0.
2. the process for preparing health-care oral liquid that diabetes patient according to claim 1 uses is characterized in that: described production craft step is:
(1.) the Cordyceps mycelium submerged fermentation is cultivated
1.1 culture medium preparation:
(a) expanding species is cultivated: the female culture medium of test tube: PDA culture medium
(b) shake-flask seed culture medium: Semen Glycines powder 2--8%, sucrose 1--5%, potassium dihydrogen phosphate 0.1--1%, the beautiful 0.05--0.5% of sulphuric acid
(c) fermentation tank culture medium: Semen Glycines powder 5--12%, sucrose 1--5%, potassium dihydrogen phosphate 0.1--1%, the beautiful 0.05--0.5% of sulphuric acid, pH nature
------1.2 shake-flask seed cultivation---fermentor cultivation---fermentation stops strain deep layer fermenting process flow process: the Cordyceps preservation strain of directive breeding
1.3 the main points of work:
1.3.1 cultivating, shake-flask seed adopts rotary shaking table, 100 times/min of shaking table frequency, inoculum concentration 10-15%, through 3-4 days, after the 25-27 ℃ of constant temperature culture, it was pale yellow little turbid to ferment, form the mycelium pellet of the about 0.2-0.5cm size of diameter, fermentation liquid has a kind of inherent delicate fragrance to shake flavour of a drug.
1.3.2 temperature:
When Cordyceps submerged fermentation cultivation optimum temperature was 25-27 ℃, when temperature was lower than 25 ℃, mycelial growth obviously was suppressed; Be higher than the easy old and feeble self-dissolving of 28C mycelial growth, culture fluid darkens.
1.3.3.:
The Cordyceps deep layer is cultivated and is required to have a meta-acid environment, and this has often considered when selecting culture medium constituent, has not needed during preparation to test pH value specially, and give free rein to.
1.3.4 ventilation:
Ventilation is cultivated very important to the Cordyceps deep layer, general ventilation is with 1: 0.5-1: 1.0/min is advisable.Bubbling air is too much or very few, all is unfavorable for mycelial growth.
1.3.5
The ventilation deep layer is cultivated jar standard of putting: hyphae content " 15% (weight in wet base): abnormal smells from the patient delicate fragrance: residual total sugar " 1.2%; Ammoniacal nitrogen<30mg/ml; Fermentation liquid is thickness very, shape such as paste; The mycelia aging, minority self-dissolving, cultivation cycle 3-4 days.
1.3.6 biological processes:
Mycelium through broken, is added the protein pectase. the compound enzyme that cellulase constitutes is 1%, 55C enzymolysis 60 minutes, 95-100 ℃, 3-5min, enzyme denaturing adds 5-10 times of water, and 90-100 ℃ of water is carried 1.5-2 hour, and high speed centrifugation carries out solid-liquid separation.Residue is handled with homogenizer, added 3-5 times of water, 90-100 ℃, extracted 1-1.5 hour, high speed centrifugation separates, clear liquid and enzymolysis solution
Merge, molten the contracting of reducing pressure obtains the Cordyceps extracting solution.
(2.) Chinese medicine processing decocts:
2.1 Radix Ginseng is pulverized, add 5-8 and doubly measure 50-75% ethanol, water-bath refluxed 2 hours, put the double-deck filter paper filtering in cold back, keep filtrate, medicinal residues extract 4 times equally, merge 5 times alcohol extract, the double-deck filter paper of reuse and to add Pulvis Talci be the filter aid filtration, behind the filtrate recycling ethanol, be condensed into syrupy shape, standby.
2.2 its body herbal raw material pulverize separately becomes diameter 2.0-3.0 mm granules in will filling a prescription, sneaking into the ginseng residue drops in the extraction pot by formulation ratio, add 5-10 times of water, soak 2-3h, then in 90-100 ℃, decoct 60-90min, filter, filtering residue adds 3-5 times of water again in 90-100 ℃, decocts 30min, filter merging filtrate.
(3.) allotment. concentrate:
With Chinese medicine extraction liquid, Chinese caterpillar fungus fermentation extracting solution mix homogeneously injects the cryogenic vacuum concentration tank and concentrates.Cycles of concentration is 1.5-2.5 a times of stock solution, and soluble solid all reaches 6-8% (20 ℃ of refractometers are measured).Mix Lac regis apis in proportion, fully stir, filter.PH is 5.0-6.0.
CN 00131110 2000-10-29 2000-10-29 Process for preparing health-care oral liquid for diabetics Pending CN1350860A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1303909C (en) * 2004-04-28 2007-03-14 中国食品发酵工业研究院 Tonify soup of aweto and its production method
CN103483040A (en) * 2013-09-05 2014-01-01 东方中科生命科学有限责任公司 Culture medium for large-scale submerged fermentation for cordyceps sinensis and fermentation production method thereof
CN103497876A (en) * 2013-09-28 2014-01-08 武汉正立源科技有限公司 Health care medicinal liquor
CN104544438A (en) * 2015-01-13 2015-04-29 李为国 Functional plant fermented beverage and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1303909C (en) * 2004-04-28 2007-03-14 中国食品发酵工业研究院 Tonify soup of aweto and its production method
CN103483040A (en) * 2013-09-05 2014-01-01 东方中科生命科学有限责任公司 Culture medium for large-scale submerged fermentation for cordyceps sinensis and fermentation production method thereof
CN103483040B (en) * 2013-09-05 2015-08-05 东方中科生命科学有限责任公司 Chinese caterpillar fungus pilot scale liquid submerged fermentation substratum and fermentation method for producing thereof
CN103497876A (en) * 2013-09-28 2014-01-08 武汉正立源科技有限公司 Health care medicinal liquor
CN104544438A (en) * 2015-01-13 2015-04-29 李为国 Functional plant fermented beverage and preparation method thereof
CN104544438B (en) * 2015-01-13 2017-05-03 李为国 Functional plant fermented beverage and preparation method thereof

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