CN102428832B - Fungal pharmaceutical mycoplasm with blood sugar lowering efficacy and preparation method thereof - Google Patents
Fungal pharmaceutical mycoplasm with blood sugar lowering efficacy and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of biofermentation engineering. A pharmaceutical medium and a fungal strain which have blood sugar lowering efficacy are used as bidirectional fermentation raw materials, wherein the pharmaceutical medium is mainly composed of the following raw materials: cornel, gynostemma, momordica grosvenori, winged euony twigs, balsam pear and polyrhachis vicina; the fungal strain is composed of the following strains: inonotus obliquus, cordyceps, phellinus igniarius, polystictus versicolor, and grifola frondosa; and bi-directional multi-fungi fermentation is carried out between multiple edible and pharmaceutical fungi and Chinese herbal medicines by a bidirectional fermentation process, thus organisms in two kingdoms or three kingdoms are organically combined to obtain an entirely new blood-sugar-lowering pharmaceutical mycoplasm product. The obtained product can generate 1+1>2 physiological function efficiency for hypoimmunity, has the actions of obviously lowering blood sugar but not increasing insulin concentration, has significant positive effects on helping treating and controlling diabetic complications, and can be used as an oral medicament for preventing and treating diabetes or a health-care product for adjusting blood sugar.
Description
Technical field
The invention belongs to the biological fermentation engineering field, be specifically related to a kind of fungi fermentation medicinal fungal substance with effect of lowering blood sugar.
Background technology
The treating diabetes difficulty is large, and expense is high, has become the global problem that national governments pay close attention to.The medicine of specially for treating diabetes only has six or seven kinds on China's medical market at present, is divided into three major types: biological medicament insulin, chemical drugs sulfonylurea biguanides, and Chinese patent drug class.Insulin preparation is expensive, is difficult to promote and have 8 large side effects; Though the chemical drugs blood sugar reducing function is obvious, and is larger to toxic and side effects such as liver, kidneys, also easily cause that cardiovascular complication increases the weight of etc.Though Chinese patent drug has certain therapeutic action, present clinical use mostly be crude extract preparation or doctor from the prescription decoction of medicinal ingredients of intending, lack drug effect significantly, active ingredient and the mechanism of action is clear, quality controllable, preparation is advanced modern Chinese herbal medicine.At present, the research range of Chinese patent drug is many in following ranges, has equally the problems referred to above:
One class is with different herbal prescriptions, or the Chinese patent drug that oven dry is pulverized or decocting is produced by conventional method.
Such as patent CN02157912.1 " ant glucose in urine ball and processing method thereof ", with ginseng, ant, cicada moves back, and the traditional Chinese medicines such as the coptis are batching, after drying pulverizing, the water decocting and concentrating, mixed powder and liquid granulation.
Patent 200910094733 " a kind of Chinese medicinal capsule for the treatment of diabetes " is characterized in that containing 23 kinds of Chinese herbal medicines such as green leaf chrysanthemum, imposing, gall nut, sealwort, American Ginseng.
Patent (AP) 02133230 " a kind of Yusanxiao medicine for the treatment of diabetes and preparation method＞" be with Radix Angelicae Sinensis, the coptis, red ginseng, pilose antler respectively pulverizing mix with the water extract of the 13 flavor Chinese medicines such as the Radix Astragali, glutinous rehmannia, prepared rhizome of rehmannia.
Patent 201010188883 " a kind of medicine for the treatment of diabetes and preparation method thereof " is bulk drug by following medicine: radix bupleuri, the root of herbaceous peony, the rhizome of nutgrass flatsedge, moutan bark etc., use Chinese medicine with coarse food grain mixture and nutrients compatibility.
Equations of The Second Kind is take Chinese medicinal plant as main, and Traditional Method processing is equipped with the synthetic drug of chemical drugs composition.
Belong to medicinal preparation such as patent CN97103536.9 " a kind of medicine for the treatment of diabetes and preparation method thereof ", inventive features is by kinds of traditional Chinese medicines: pig a kind of reed mentioned in ancient books, rhizoma alismatis, golden cypress, glutinous rice bud point etc. dissolve Metformin hydrochloride, Gliquidone through drying frying.
The 3rd class is the compound medicine with the Chinese medical extract compatibility.
Mainly formed by ethyl acetate extract or the extract of Ramulus euonymi such as patent CN02138362.6 " a kind of Chinese medicament for reducing blood sugar and preparation method thereof ".This drug main of patent 200610066305 " a kind of pharmaceutical composition for the treatment of diabetes and preparation method thereof " will add chromium picolinate through the alcohol reflux extraction by tartarian buckwheat, mulberry leaf combination and make.
Up to now, the research that the edible and medicinal fungi biologic product is used for the treatment of diabetes and complication aspect thereof is still few, the medicinal fungal substance goods that more useless two-way fermentation engineering obtains." medicinal fungi biologic product and the preparation technologies thereof for the treatment of diabetes " are formulated by the extract of wild mushroom extract, mulberry leaf and Chinese yam mixture such as patent 200810064886.Patent ZL03111130.0 " edible and medicinal fungi solid fermentation bacterium powder " also only limits to do the mycelium that solid fermentation obtains with nutrient matrix and forms through compatibility.
Summary of the invention
The present invention adopts is the two-way fermentation technique of microorganism and " many bacterium " fermentation technique of not yet relating to of diabetes field so far.Namely do property of medicine matrix with Chinese herbal medicine, between many edible and medicinal fungis and Chinese herbal medicine, carry out amphicheirality's mixed culture fermentation, make the brand-new hypoglycemic property of medicine mycoplasma that organically combines between two or three boundary's biologies and obtain.
Theoretical foundation of the present invention is as follows:
A: two-way fermentation engineering refers to, fermentation substrate all adopts Chinese herbal medicine (or adding therein an amount of agricultural byproducts) and consists of property of medicine matrix, use medicinal fungi fermentation again, cause fermentation to have the amphicheirality, its tunning transfers medicinal fungal substance to by simple fungal mycelium.This medicinal fungal substance is added with better drug effect simply mutually than onset (original) fungi or property of medicine matrix itself or both, can produce the physiological function effectiveness of 1+1＞2, can significantly strengthen its active component, improves the effects such as drug effect and curative effect.
The two-way zymotechnique that the present invention adopts, that fermented bacterium and property of medicine matrix are consisted of in the fermentation combination, its property of medicine matrix was rich in the agricultural byproducts of the nutrition such as carbon, nitrogen as nutrient matrix by only use in the past, change into to adopt and have the traditional Chinese medicine of certain hypoglycemic activity composition as property of medicine matrix, it can provide for the fungi with function of reducing blood sugar the growth desired nutritional, enzyme because of fungi is changed structural constituent again, thereby produces new nature,taste and action.Because the present invention is conversion and the combination of carrying out the organic active composition between two boundaries or three boundary's biologies, its functional component is abundanter again, and hypoglycemic effect is more obvious.
Technological process of the present invention is as follows:
Take by weighing property of medicine base starting material → add water → sterilization → pro-rata property of medicine matrix → access respectively fungi strain → all medicinal fungal substances of two-way fermentation → collection → oven dry to pulverize → obtain the medicinal fungal substance goods.
B: adopt multiple edible and medicinal fungi with remarkable hypoglycemic activity composition and no side effects as fermented bacterium: Inonotus obliquus (Inonqqus obliquus); Cordyceps sinensis [Cordyceps sinensis (Berk.) Sacc]; Phellinus [(pHellinus igniariusL.es Fr.) Quel.], grifola frondosus (Grifola frondosa), rainbow conk [Coriolus versicolor (L.:Fr.)].Above-mentioned bacterial classification all derives from Dalian, Liaoning Province precious gill fungus fungus research and development centre.
The function of blood sugar reduction of the various fungies of adopting is as follows:
Inonotus obliquus: Inonotus obliquus is at Russia's medicinal fungi as the treatment disease among the people, and long-term animal experiment and clinical testing show without any side effects, and hepatitis, hypertension are also had control efficiency.Ceng Xiaolong's, " chemical composition of Inonotus obliquus and pharmacological research " shows, its artificial cultured mycelia contains a large amount of hypoglycemic, vegetable fiber class polysaccharide bodies of bringing back to life immunization, and the cure rate of diabetes is reached 93%.
Cordyceps sinensis: according to " researchs of Edible Fungal Polysaccharide on Reducing Blood Sugar mechanism " such as Du Mei, its main medicinal ingredient cordycepin has inhibitory action to hyperglycaemia, two kinds of polysaccharide Chinese caterpillar fungus hypha heteroglycan CsOH (being the heteroglycan that contains Arabinose, L-wood sugar, D-MANNOSE, D-galactose and D-Glucose) and polysaccharide semifinished product CHWc can significantly improve (containing D-MANNOSE, D-galactose and D-Glucose) physiologically active of beta Cell of islet, and hyperglycaemia is had obvious reducing effect.And can protect kidney to improve the immunity of human body, effectively control the various complication of diabetes.
Phellinus: the IL-1 cytokine that the Phellinus polysaccharide excites, the promotion insulin secretion is arranged, reduce the effect of blood sugar.Contain simultaneously abundant amino acid and zinc, have the effect of activation pancreas function.But and the immunocompetence of Effective Raise patient of diabetes.The Phellinus bacterium is promoted in addition cell function, reduces cholesterol, antithrombotic, and the effects such as contraction of mitigation blood vessel all help diabetic's blood pressure normalization (seeing " the domestic and international progress of Rare Medicinal Fungus Phellinus sp " such as Sun Pei dragons).
Disclose among grifola frondosus: the Du Mei etc. " research of Edible Fungal Polysaccharide on Reducing Blood Sugar mechanism ", the functional component of grifola frondosus mainly is polysaccharide and glycoprotein, for a kind of β of originated from fungus-(1,3)-D-glucan, has and falls hypoglycemic function.The Organic Chromium that content is higher in the grifola frondosus and Organic Selenium have the effect that assist insulin is kept normal sugar tolerance simultaneously.Also have in addition the active cell immunologic function, reduce blood pressure, the function of blood fat and serum insulin.Cirrhosis, diabetes are all produced effect and without any side effects.
Rainbow conk: contained polysaccharide in the rainbow conk (polysaccharide of Corlolus versicolor, PSK) has control blood sugar rising effect, on euglycemia without impact.Oral medication reacts without long term toxicity.Experiment shows that PSK is the very low material of a toxicity, also without mutagenesis (seeing " researchs of Edible Fungal Polysaccharide on Reducing Blood Sugar mechanism " such as Du Mei).
C: choose have effect of reducing blood sugar wide with, applicable crowd, have no side effect, on normal blood glucose value without the Chinese herbal medicine of impact (not reducing) or animal as property of medicine matrix:
Dogwood: the contained total terpene of dogwood can improve the normal mouse sugar tolerance, obviously reduces the blood glucose value that alloxan is induced, and improves serum insulin level; Significantly reduce the blood glucose value of streptozotocin-diabetes model, increase hepatic glycogen content.Normal blood sugar level is had no significant effect.Classified as one of Chinese herbal medicines kind (seeing " Chinese medicine blood-sugar decreasing active Recent Researchs " such as Liang Lei) by country.
Gynostemma pentaphylla: according to Xu Shiming, " research of gynostemma pentaphylla and application " record of Guo Yuxiao, the hypoglycemic activity of Gynostemma pentaphylla polysaccharide is different from general antidiabetic drug, its energy delay glucose is in the absorption of enteron aisle, Inhibiting α-glucosidase is active, can reduce the hyperglycaemia sufferer that alloxan induces and improve glycometabolism.Euglycemia without impact, is protected suprarenal gland, thymus gland and endocrine organ's effect in addition, hypotensive blood fat, control complication.
Momordica grosvenori: Qi Xiangyang etc. " Momordica grosvenori is to the hypoglycemic activity of diabetic mice " studies show that, it contains abundant glucoside, and the T2DM-II type diabetes that this glucoside is induced alloxan have remarkable hypoglycemic activity, and on normal mouse blood sugar without impact.Prevent and treat in addition hypertension, reducing blood lipid and antiobesity action, can control complication.
Report in " the Ramulus euonymi pharmaceutical research is brief talked " of Ramulus euonymi: Yin Guiyu, Yang Jinping, it contains the multiple hypoglycemic composition that falls, and can obviously reduce the blood glucose value that alloxan is induced, on normal mice blood sugar without impact.Its oxaloacetic acid sodium composition can stimulate the beta cell hyperplasia, adjusts abnormal metabolic process, strengthens the secretion of insulin.Nontoxic, being decocted in water for oral dose does not have apparent side effect yet for long-term taking or heavy dose (30g is following).Can also treat the diseases such as coronary heart disease, hypertension, arteriosclerosis, the complication of diabetes is had control action.
Balsam pear: its momordica saponins is the main active ingredient of hypoglycemic, can obviously reduce alloxan diabetes patient's blood sugar, and total effective rate reaches 82% and without toxicity (seeing " Chinese medicine blood-sugar decreasing active Recent Researchs " such as beam thunder, limit Po Lams).
Polyhachis vicina Roger: there are some researches show, it is to have the new resource food that medicine-food two-purpose is worth, and is the bidirectional immune regulator that can keep the organism physiology balance, can two-way adjustment blood sugar and blood pressure.Contained biologically active chromium can be kept sugar tolerance by assist insulin." cursive script formaldehyde " and contained extraordinary insect protein; can stimulate forcefully body to produce protection antibody, can make the diabetic avoid the infringement (seeing " researchs of several Animal Medicine hypoglycemic activities " such as the Ren Geng that relaxes) of various organs with sterol and triterpene compound.
The present invention specifically realizes in the following manner:
An aspect of of the present present invention is: the present invention relates to a kind of raw material with fungi medicinal fungal substance of effect of lowering blood sugar, comprises property of medicine matrix and fungi strain, wherein:
Property of medicine matrix is comprised of following raw material: dogwood, Gynostemma pentaphylla, Momordica grosvenori, Ramulus euonymi, balsam pear, Polyhachis vicina Roger, nutrient matrix; Wherein, the source of nutrient matrix is cereal, beans, rice bran, wheat bran;
Fungi strain is comprised of following bacterial classification: Inonotus obliquus, Cordyceps sinensis, Phellinus bacterium, rainbow conk, grifola frondosus;
The inoculation weight of fungi strain is 8～12wt% of property of medicine matrix.
In the raw material of above-mentioned fungi medicinal fungal substance, when property of medicine matrix and fungi strain during in following ratio proportioning, effect is better:
Property of medicine matrix is mixed by mass percentage by the raw material of following drying:
The corresponding relation of each fungi strain and used property of medicine matrix is respectively by mass percentage:
The inoculation weight of fungi strain is 8～12wt% of property of medicine matrix.
In the raw material of above-mentioned fungi medicinal fungal substance, when property of medicine matrix and fungi strain during in following ratio proportioning, best results:
Property of medicine matrix is mixed by mass percentage by the raw material of following drying:
The corresponding relation of each fungi strain and used property of medicine matrix is respectively by mass percentage:
The inoculation weight of fungi strain is the 10wt% of property of medicine matrix.
Other one side of the present invention is: a kind of preparation method with fungi medicinal fungal substance of effect of lowering blood sugar, and its concrete technology step is as follows:
(e) take by weighing by mass percentage dry property of medicine base starting material and mixing;
(f) in the mixture of step a gained, the running water of the pH7 of 1～10 times of mixture quality of adding～8 in 100～127 ℃ of sterilization 1～10h, obtains property of medicine matrix;
(g) by the corresponding relation of each fungi strain and used property of medicine matrix, the property of medicine matrix of allocation step b gained accesses respectively fungi strain under aseptic condition, inoculative proportion is 8～12wt% of property of medicine matrix, 23～27 ℃ of two-way fermentations 4～45 days, obtain respectively medicinal fungal substance;
(h) collect all medicinal fungal substances that step c obtains, mix obtaining this product after oven dry is pulverized.
Concrete: among the preparation method of above-mentioned fungi medicinal fungal substance, the two-way fermentation mode of employing is solid fermentation or liquid deep layer fermenting.
Concrete: among the preparation method of above-mentioned fungi medicinal fungal substance, the liquid deep layer fermenting condition is: 25～27 ℃ of temperature, tank pressure 0.7～0.8kg/cm
2, throughput 1: 1v/v/ divides.
Concrete: among the preparation method of above-mentioned fungi medicinal fungal substance, the inoculative proportion of described fungi strain is the 10wt% of property of medicine matrix.
Other one side of the present invention is: by the fungi medicinal fungal substance of said method preparation.
Other one side of the present invention is: the application of above-mentioned fungi medicinal fungal substance in preparation electuary, pill, tablet, capsule.
The liquid deep layer fermenting technology of edible mushroom belongs to one of modern biotechnology.The submerged fermentation technology is directly produced the edible mushroom thalline, obtains to be rich in simultaneously the zymotic fluid of the nutrient components such as amino acid and secondary metabolites.The key of edible fungus liquid submerged fermentation technical research is, different edible mushrooms will be cultivated with different fermentation substrates, and therefore, selecting with the preparation fermentation substrate is the key of edible fungus liquid submerged fermentation technology.Utilize food (medicine) can obtain a large amount of mycelium and tunning thereof within a short period of time with the bacteria liquid submerged fermentation, because this Period Process is short, output is high, cost is low, process equipment is simple, therefore in Edible Fungi, have broad application prospects.Food (medicine) can produce the several physiological active substances such as the multiple compounds of polysaccharide, alkaloid, terpenoid, sterol, enzyme, nucleic acid, vitamin, the effect of tool antibiotic and plant hormone in the submerged fermentation process with fungi, these materials have respectively to the effect of preventing and curing diseases of the human organs such as cardiovascular, liver, nervous system, kidney, property and anticancer, anti-inflammatory, anti-ageing, antibiotic, improve the effect such as immunity.
The product that the present invention relates to adopts edibleization that organise, the pollution-free property of medicine matrix that has no side effect and various fungi, and in whole production technology, do not add and use any chemical substance, to guarantee the safety of product, avoid in treatment and health care, human body being formed secondary injury.
The present invention organically combines the composition of the interbiotic main hypoglycemic factor cordycepin in three boundaries, multiple fungi polysaccharide and multiple medicinal plant polysaccharide and biological chromium through amphicheirality's fermentation and physiological conversion, forms to have new bioactive molecule group and the medicinal fungal substance of secondary metabolites.
Beneficial effect: the invention belongs to biomedicine field.The two-way fermentation technique of microorganism and mixed culture fermentation technology that the diabetes field so far of adopting not yet relates to make to organically combine between two or three boundary's biologies and obtain brand-new hypoglycemic mycoplasma.This product has the effect that obvious reduction blood sugar does not increase again insulin concentration, for supplemental treatment and the complication of controlling diabetes obvious and positive effect is arranged, and does not cause the acute hypoglycemia that Common drugs produces.Quality controllable than existing blood-sugar lowering tcm drug, formulation is advanced.This product belongs to pure biologic product, and is without any side effects; Said preparation is suitable for diabetic's treatment, the prevention of diabetes, can be used as the health medicine that falls hypoglycemic medicinal preparation for oral administration or regulate blood sugar.
Embodiment
The raw material sources that relate in the following non-limiting example are as follows:
Dogwood, Gynostemma pentaphylla, Momordica grosvenori, Ramulus euonymi, all be purchased from the shop of Chinese medicines;
Balsam pear is purchased from Shanghai Yi Lin Food Co., Ltd;
Polyhachis vicina Roger derives from the pure ant powder of Hengxian County factory;
Fermented bacterium: Inonotus obliquus (Inonqqus obliquus); Cordyceps sinensis [Cordyceps sinensis (Berk.) Sacc]; Phellinus [(pHellinus igniariusL.ex Fr.) Quel.], grifola frondosus (Grifola frondosa), rainbow conk [Coriolus versicolor (L.:Fr.)].Above-mentioned bacterial classification all derives from Dalian, Liaoning Province precious gill fungus fungus research and development centre;
Laboratory animal: kunming mice, small white mouse, body weight 18～22g, male and female half and half are purchased from the good universe journey bio tech ltd in Shenyang.
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
(1) preparation of medicinal fungal substance
(a) with Chinese herbal medicine after 50～60 ℃ of oven dry, take by weighing dry property of medicine base starting material and mix by following mass percent: dogwood 2000g, Gynostemma pentaphylla 3000g, Momordica grosvenori 1500g, Ramulus euonymi 1500g, balsam pear 1500g, Polyhachis vicina Roger 500g.Above-mentioned raw materials is crushed to 1～2mm.
(b) mixture of step a gained is packed into container adds the running water of pH7.5 by 1: 1 weight ratio, in 127 ℃ of sterilization 2h, be cooled to below 30 ℃ after, obtain property of medicine matrix.
(c) under aseptic condition, in 2000g property of medicine matrix, access the Inonotus obliquus bacterial strain; In 4000g property of medicine matrix, access Cordyceps strain; In 2000g property of medicine matrix, access the Phellinus bacteria strain; In 1000g property of medicine matrix, access Grifola frondosa strain; In 1000g property of medicine matrix, access the rainbow conk bacterial strain; The inoculative proportion of above-mentioned bacterial classification is 10% of the property of medicine matrix weight inoculated.
(d) connect 25 ± 1 ℃ of fermentations of bacterium postposition 45 days.
(e) collect all medicinal fungal substances that steps d obtains, dry to water content below 5% through 40～50 ℃, mix being crushed to below the 100nm by the nano cell wall-broken grinder, obtain the medicinal fungal substance goods.
(2) finished product detects
According to appendix VID of Pharmacopoeia of People's Republic of China version in 2005, high performance liquid chromatography is measured cordycepin and is reached 3.88mg/g; Measuring total polysaccharide according to UV-VIS spectrophotometry (appendix VA of Chinese Pharmacopoeia version in 2005) is 16.7%; According to the assay method of chromium content in standard GB/T/T5009.123-2003 food, measuring biological chromium content is 0.86mg/kg.
Embodiment 2
(1) preparation of medicinal fungal substance
(a) with Chinese herbal medicine after 50～60 ℃ of oven dry, take by weighing dry property of medicine base starting material and mix by following mass percent: dogwood 1000g, Gynostemma pentaphylla 2500g, Momordica grosvenori 1000g, Ramulus euonymi 2000g, balsam pear 3000g, Polyhachis vicina Roger 500g.Above-mentioned raw materials is crushed to 1～2mm.
(b) mixture of step a gained is packed into container adds the running water of pH7.5 by 1: 1 weight ratio, in 127 ℃ of sterilization 2h, be cooled to below 30 ℃ after, obtain property of medicine matrix.
(c) under aseptic condition, in 3000g property of medicine matrix, access the Inonotus obliquus bacterial strain; In 3000g property of medicine matrix, access Cordyceps strain; In 2000g property of medicine matrix, access the Phellinus bacteria strain; In 1000g property of medicine matrix, access Grifola frondosa strain; In 1000g property of medicine matrix, access the rainbow conk bacterial strain; The inoculative proportion of above-mentioned bacterial classification is 10% of the property of medicine matrix weight inoculated.
(d) connect 25 ± 1 ℃ of fermentations of bacterium postposition 45 days.
Collect all medicinal fungal substances that steps d obtains, dry to water content below 5% through 40～50 ℃, mix being crushed to below the 100nm by the nano cell wall-broken grinder, obtain the medicinal fungal substance goods.
(2) finished product detects
According to appendix VID of Pharmacopoeia of People's Republic of China version in 2005, high performance liquid chromatography is measured cordycepin and is reached 3.3mg/g; Measuring total polysaccharide according to UV-VIS spectrophotometry (appendix VA of Chinese Pharmacopoeia version in 2005) is 14.6%; According to the assay method of chromium content in standard GB/T/T5009.123-2003 food, measuring biological chromium content is 0.89mg/kg.
Embodiment 3
(1) preparation of medicinal fungal substance
(a) with Chinese herbal medicine after 50～60 ℃ of oven dry, take by weighing dry property of medicine base starting material and mix by following mass percent: dogwood 2400g, Gynostemma pentaphylla 2000g, Momordica grosvenori 2000g, Ramulus euonymi 500g, balsam pear 1000g, Polyhachis vicina Roger 100g.After above-mentioned raw materials is crushed to 1～2mm, add rice 2000g.
(b) mixture of step a gained is packed into container adds the running water of pH7.5 by 1: 1 weight ratio, in 127 ℃ of sterilization 2h, be cooled to below 30 ℃ after, obtain property of medicine matrix.
(c) under aseptic condition, in 4000g property of medicine matrix, access the Inonotus obliquus bacterial strain; In 2000g property of medicine matrix, access Cordyceps strain; In 2000g property of medicine matrix, access the Phellinus bacteria strain; In 1000g property of medicine matrix, access Grifola frondosa strain; In 1000g property of medicine matrix, access the rainbow conk bacterial strain; The inoculative proportion of above-mentioned bacterial classification is 10% of the property of medicine matrix weight inoculated.
(d) connect 25 ± 1 ℃ of fermentations of bacterium postposition 45 days.
(e) collect all medicinal fungal substances that steps d obtains, dry to water content below 5% through 40～50 ℃, mix being crushed to below the 100nm by the nano cell wall-broken grinder, obtain the medicinal fungal substance goods.
(2) finished product detects
According to appendix VID of Pharmacopoeia of People's Republic of China version in 2005, high performance liquid chromatography is measured cordycepin and is reached 1.3mg/g; Measuring total polysaccharide according to UV-VIS spectrophotometry (appendix VA of Chinese Pharmacopoeia version in 2005) is 11%; According to the assay method of chromium content in standard GB/T/T5009.123-2003 food, measuring biological chromium content is 0.21mg/kg.
Embodiment 4
(1) preparation of medicinal fungal substance
(a) with Chinese herbal medicine after 50～60 ℃ of oven dry, take by weighing dry property of medicine base starting material and mix by following mass percent: dogwood 600g, Gynostemma pentaphylla 600g, Momordica grosvenori 500g, Ramulus euonymi 600g, balsam pear 500g, Polyhachis vicina Roger 200g.Above-mentioned raw materials is crushed to 100 orders.
(b) mixture of step a gained is packed into fermentation tank adds the running water of pH7.5 by 1: 10 weight ratio, in 127 ℃ of sterilizations 40 minutes, be cooled to below 30 ℃ after, obtain property of medicine matrix.
(c) under aseptic condition, according to the 10wt% access Inonotus obliquus bacterial strain of the property of medicine matrix weight of inoculating.
(d) meet 25 ± 1 ℃ of bacterium postposition, tank pressure 0.7～0.8kg/cm
2, throughput 1: 1v/v/ divides, fermented and cultured 8 days.
(e) collect steps d and obtain the Inonotus obliquus medicinal fungal substance, with haplo-effect concentrator Vacuum Concentration to 1.2, dry to water content below 5% through 40～50 ℃, obtain the Inonotus obliquus medicinal fungal substance.
(f) submerged fermentation method with above-mentioned Inonotus obliquus obtains respectively aweto, Phellinus bacterium, the corresponding medicinal fungal substance of grifola frondosus, rainbow conk.
(g) get Inonotus obliquus medicinal fungal substance 2000g; aweto medicinal fungal substance 1500g, Phellinus bacterium medicinal fungal substance 500g, grifola frondosus medicinal fungal substance 500g, rainbow conk medicinal fungal substance 500g; mixing is crushed to below the 100nm by the nano cell wall-broken grinder, obtains the medicinal fungal substance goods.
(2) finished product detects
According to appendix VID of Pharmacopoeia of People's Republic of China version in 2005, high performance liquid chromatography is measured cordycepin and is reached 2.5mg/g; Measuring total polysaccharide according to UV-VIS spectrophotometry (an appendix V of Chinese Pharmacopoeia version in 2005 A) is 18.7%; According to the assay method of chromium content in standard GB/T/T5009.123-2003 food, measuring biological chromium content is 0.43mg/kg.
Embodiment 5
(1) preparation of medicinal fungal substance
(a) with Chinese herbal medicine after 50～60 ℃ of oven dry, take by weighing and mix by following mass percent: dogwood 3000g, Gynostemma pentaphylla 2000g, Momordica grosvenori 1000g, Ramulus euonymi 1500g, balsam pear 1500g, Polyhachis vicina Roger 1000g.With said mixture through three lixiviates: after the running water that adds the pH7.5 of 2 times of mixture weight is for the first time boiled 20 minutes, collect filtrate, the running water that adds for the second time the pH7.5 of 2 times of mixture weight was boiled rear 15 minutes, collect filtrate, the running water that adds for the third time the pH7.5 of 2 times of mixture weight was boiled rear 10 minutes, collected filtrate, then three times filtrate was merged, be concentrated into 6000ml, it is joined in the 5000 gram rice.
(b) mixture of step a gained is packed into container, in 127 ℃ of sterilization 2h, be cooled to below 30 ℃ after, obtain property of medicine matrix.
(c) under aseptic condition, in 500g property of medicine matrix, access the Inonotus obliquus bacterial strain; In 2000g property of medicine matrix, access Cordyceps strain; In 500g property of medicine matrix, access the Phellinus bacteria strain; In 1000g property of medicine matrix, access Grifola frondosa strain; In 1000g property of medicine matrix, access the rainbow conk bacterial strain; The inoculative proportion of above-mentioned bacterial classification is 10% of the property of medicine matrix weight inoculated.
(d) connect 25 ± 1 ℃ of fermentations of bacterium postposition 45 days.
(e) collect all medicinal fungal substances that steps d obtains, dry to water content below 5% through 40～50 ℃, mix being crushed to below the 100nm by the nano cell wall-broken grinder, obtain the medicinal fungal substance goods.
(2) intermediate products detect
The polysaccharide of measuring property of medicine matrix according to UV-VIS spectrophotometry (appendix VA of Chinese Pharmacopoeia version in 2005) is 6.7%, and the polysaccharide of gained medicinal fungal substance increases to 9.1% after the Inonotus obliquus fermentation; According to the assay method of chromium content in standard GB/T/T5009.123-2003 food, measure the biological chromium content 0.73mg/kg of property of medicine matrix, the medicinal fungal substance after the grifola frondosus fermentation is brought up to 1.16mg/kg.According to appendix VID of Pharmacopoeia of People's Republic of China version in 2005, high performance liquid chromatography is measured cordycepin content and is brought up to 9.2mg/g in the Chinese caterpillar fungus mycoplasma by the 0mg/g in its property of medicine matrix.
Thus explanation, be added with simply mutually better drug effect through the medicinal fungal substance of two-way fermentation than onset (original) fungi or property of medicine matrix itself or both, can produce the physiological function effectiveness of 1+1＞2, can significantly strengthen its active component, improve the effects such as drug effect and curative effect.
(3) finished product detects
According to appendix VID of Pharmacopoeia of People's Republic of China version in 2005, high performance liquid chromatography, cordycepin reaches 4.3mg/g; Measuring total polysaccharide according to UV-VIS spectrophotometry (an appendix V of Chinese Pharmacopoeia version in 2005 A) is 20.6%; According to the assay method of chromium content in standard GB/T/T5009.123-2003 food, measuring biological chromium content is 3.8mg/kg.
Embodiment 6 zooperies
Divide into groups: after the confession examination kunming mice of 54 body weight 18～22g is raised 5 days, be divided at random three groups every group 18 male and female half and half.Three groups are respectively blank group, hyperglycemia model group, fungi-medicine group.Except the blank group, other two groups of mouse with 200mg/kg dosage injection alloxan, induce hyperglycaemia to reach 14.4mmol/L for three days on end.
Dispenser: blank group and hyperglycemia model group mouse be with continuous normal saline gavage 30 days, and the fungi-medicine group was with the continuous gavage of 25mg/kg dose 30 days.Then respectively organize the eyeball of mouse blood sampling and measure mice serum glucose with blood sugar test paper (commercially available).
The result: the zooperal result of above-mentioned small white mouse such as table 1, fungi-medicine group are compared difference and are had statistical significance (P＜0.05) with model group, the fungi-medicine group is compared difference not statistically significant (P＞0.05) with normal group.This result proves, product of the present invention can significantly reduce in the Mice Body, the hyperglycaemia of inducing through alloxan (200mg/kg).
Glucose content in table 1. mice serum (mmol/L) testing result
Claims (3)
1. the raw material with fungi medicinal fungal substance of effect of lowering blood sugar comprises property of medicine matrix and fungi strain, it is characterized in that:
Property of medicine matrix is comprised of following raw material: dogwood, Gynostemma pentaphylla, Momordica grosvenori, Ramulus euonymi, balsam pear, Polyhachis vicina Roger, nutrient matrix; Wherein, the source of nutrient matrix is cereal, beans, rice bran, wheat bran;
Fungi strain is comprised of following bacterial classification: Inonotus obliquus, Cordyceps sinensis, Phellinus bacterium, rainbow conk, grifola frondosus;
Wherein, the inoculation weight of fungi strain is 8～12wt% of property of medicine matrix.
2. the raw material of fungi medicinal fungal substance according to claim 1 is characterized in that:
Property of medicine matrix is mixed by mass percentage by the raw material of following drying:
The corresponding relation of each fungi strain and used property of medicine matrix is respectively by mass percentage:
The inoculation weight of fungi strain is 8～12wt% of property of medicine matrix.
3. the raw material of fungi medicinal fungal substance according to claim 2 is characterized in that:
Property of medicine matrix is mixed by mass percentage by the raw material of following drying:
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