The object of the invention is to provide a kind of preparation method with physiologically active medicine-junzhisu bacteria liquid mixture, not only have the effective ingredient of former medicinal plants and former medicinal fungi with the junzhisu bacteria liquid mixture of this method preparation, and possess super former pharmaceutically-active special drug effect through biology mixings coordinating effect one-tenth; It can regulate body fluid, coordinates the normal state fertility effect of flora in the body, promotes and control cell growth promoter, thereby can cure multiple difficult and complicated illness and antagonism disease in the short period of time.
The alleged junzhisu bacteria liquid mixture of the present invention refers to the mixed liquor through the medicinal fungus liquid of the variety classes various dose of special biotechnical processes method preparation.
The technical scheme that realizes the object of the invention is: utilize medicinal plants to be the culture medium culturing medicinal fungi, extract through biological zymolysis, after being prepared into physiologically active medicinal fungus liquid,, be mixed with variety classes and various dose medicinal fungus liquid mixture is promptly made the junzhisu bacteria liquid mixture finished product again according to clinical needs.Junzhisu bacteria liquid mixture is concrete preparation technology comprise: one, the medicinal fungi bacterial strain is chosen; Two, bacterial strain mutation; Three, bacterial strain acclimatization culture; Four, bacterial strain production is cultivated; Five, biological zymolysis is extracted; Six, medicinal fungus liquid filtration sterilization; Seven, step such as finished product preparation.Now concrete steps are described below:
One, the medicinal fungi bacterial strain is chosen: medicinal fungi bacterial strain of the present invention comprises: JINZHENGU, Auricularia, Auricularia polytricha (Mout) Sacc., Lentinus Edodes, Hericium erinaceus (Bull. Ex Fr.) Pers., Volvariella volvacea (Bull.Ex Franch.) Singer, mushroom, Ganoderma, Ganoderma, Tremella aurantialba Bandoni et Zang, Tremella, pick up the ears, medicinal fungus bacterial strain such as Poria.
Two, medicinal bacterial strain mutagenic and breeding: the medicinal fungi bacterial strain is inoculated in respectively in the PDA culture medium, put in 30 ℃ of environment and cultivated 1-5 days, put again and use the 30W quartz burner in the mutation case, distance 3-18cm shines respectively, 1-18 minutes rearmounted temperature are that cultivated 5-8 days in 28-33 ℃ of darkrooms, select the efficient medicinal bacterial strain of the surging effect of fertility.
Three, medicinal bacterial strain acclimatization culture
1, domestication culture medium preparation: the medicinal plants prescription of selecting for use as the domestication culture medium of medicinal bacterial strain is by Semen phaseoli radiati 30-40%, Semen Tritici aestivi 25-35%, Fructus Cucurbitae moschatae 7-17%, Herba Spinaciae 3.5-6.5%, Semen sojae atricolor 3.5-6.5%, Herba Imperatae 3.5-6.5, Folium Camelliae sinensis 1.5-3.5%, Herb Gynostemmae Pentaphylli 1.5-3.5%, pollen 0.5-1.5%, Fructus Jujubae (enucleation) 0.5-1.5%, Radix Morindae Officinalis 0.5-1.5%, take by weighing each raw material of culture medium, Semen phaseoli radiati, Semen sojae atricolor, Semen Tritici aestivi are dipped into rudiment, pick up and drain; Fructus Cucurbitae moschatae, Herba Spinaciae, Herba Imperatae, Radix Morindae Officinalis are chopped into 2cm size particle, after the mixing, after 6 times of left and right sides magnetic water of adding material quantity boil 30 minutes, filter and remove residue.Filtrate, add 0.18% agar of main amount of filtrate while hot, be sub-packed in 20 * 20mm in vitro after sterilization treatment, promptly make the naturalized strain culture medium.
2, the acclimatization culture of medicinal bacterial strain: will insert respectively through the efficient bacterial strain of mutagenic and breeding in the above-mentioned domestication culture medium for preparing and carry out acclimatization culture, and therefrom filter out the efficient medicinal bacterial strain that is suitable for the fertility of medicinal plants culture medium.Medicinal bacterial strain put in the incubator cultivate, therefrom filter out the bacterial strain of suitable high temperature fertility, go up use for producing.
Four, the production of medicinal bacterial strain is cultivated
1, production culture medium preparation: the culture medium prescription of medicinal bacterial strain production usefulness is with above-mentioned domestication culture medium, cultivate based raw material respectively through soaking rudiment, after the chopping mixing, add magnetic water, to water content 65%, and after regulating pH value to 6 (faintly acid), add the Gypsum Fibrosum of material quantity 2% and about 1% sucrose again, mix homogeneously is respectively charged in the 750g vial, 280-380g/ bottle, through sterilization treatment, promptly make the production culture medium of medicinal bacterial strain again.
2, the production of medicinal bacterial strain is cultivated: will insert in the above-mentioned production culture medium for preparing through the efficient medicinal bacterial strain of acclimatization culture respectively, 22-28 ℃ of temperature, carrying out productivity under relative humidity 75-85% condition cultivates, when hyphal development when presenting pigment secretions, be that mycelial growth changes cometabolism over to during the phase by elementary metabolism, be about to temperature and bring up to 40-45 ℃, mycelium stops the differentiation fertility about 2 hours, promptly gets medicinal fungus filament and metabolite thereof.
Five, zymolysis is extracted:
1, biological synergistic agent preparation: biological synergistic agent prescription is by fresh-laid egg (shelling) 0.82-2.5%, fresh Mel 1.64-3.28%, vinegar 2-3.7%, vitamin C 0.045-0.252%, zinc gluconate 0.022-0.082%, magnetic water 73.8-92.4%, mix homogeneously, regulate PH to 5-6, promptly join biological synergistic agent.
2, the monotype medicinal liquid is produced: add biological synergistic agent in above-mentioned being covered with in the mycelial compost of having stopped cultivating by 200-300ml/ bottle amount, 35-50 ℃ of following zymolysis of temperature 2-4 hours, filtration is won behind time medicinal liquid, in compost, add synergistic agent by 100-150ml/ bottle again and repeat the condition of zymolysis for the first time, filter medicinal liquid for the second time, merge first and second time medicinal liquid and be the unitary type medicinal liquid.
3, the mixed type medicinal liquid is produced: the compost that above-mentioned second extraction is crossed is formed according to clinical junzhisu bacteria liquid mixture form formula, taking out each bacterium compost in proportion puts in the stainless pot, the biological synergistic agent that adds 0.4-0.6 times of Mixed culture material amount, 45-55 ℃ of C pH values 3.5-4.5 of temperature (lactic acid adjusting) zymolysis 1.5-2.5 hours, filter to get filtrate and promptly make the mixed type medicinal liquid.
Two, filtration sterilization: the above-mentioned unitary type medicinal liquid of producing and mixed type medicinal liquid are put filter press respectively filter, filter flask is sub-packed in the container that sterilization crosses seals, put in 50-60 ℃ of water-baths and handled 3-5 hours, sharply cooling gets final product again.
Seven, finished product is produced: by clinical junzhisu bacteria liquid mixture form formula, account for 50% by the unitary type medicinal liquid, the mixed type medicinal liquid accounts for 50%, makes by different medicinal fungus liquid mixed liquors, promptly makes junzhisu bacteria liquid mixture dosage form finished product.
1, junzhisu bacteria liquid mixture A type prescription: by the unitary type medicinal liquid, Flammulina velutipes liquid, Poria liquid each 12.5-17.5%, lucidum liquid, Lentinus Edodes liquid, Auricularia liquid, pick up the ears liquid each 2.5-7.5% and the mixed type medicine 35-65% of above-mentioned mushroom, mix the mixed liquor of forming.
2, junzhisu bacteria liquid mixture Type B prescription: by unitary type medicinal liquid Flammulina velutipes liquid 12.5-17.5%, Hericium erinaceus (Bull. Ex Fr.) Pers. liquid 7.5-12.5%, Volvariella volvacea (Bull.Ex Franch.) Singer liquid, lucidum liquid, the liquid of picking up the ears, Auricularia polytricha (Mout) Sacc. liquid, tremella liquid each 2.5-7.5% and the mixed type medicinal liquid 32.5-67.5% of above mushroom, the mixed liquor of composition.
3, junzhisu bacteria liquid mixture C type prescription, by unitary type medicinal liquid Flammulina velutipes liquid, Volvariella volvacea (Bull.Ex Franch.) Singer liquid each 7.5-12.5%, the mixed liquor that the mixed type medicinal liquid of Hericium erinaceus (Bull. Ex Fr.) Pers. liquid, tremella fluid, Poria liquid, mushroom liquid, Ganoderma liquid, each above mushroom of 2.5-7.5 and 32.5-67.5 of tremella liquid is formed.
Characteristics of the present invention are:
1, the junzhisu bacteria liquid mixture of the present invention active ingredient of having had both plurality of Chinese, crude drug and medicinal fungi becomes the special effect composition through biology mixing coordinating effect, and the physiologically active height to many difficult and complicated illness and antagonism disease, has the effect of rapid healing and has no side effect again; And all can reach 100% with interior obvious effective rate to various abnormal symptoms and take a turn for the better at 15 minutes.For example: the junzhisu bacteria liquid mixture Type B follows hyperlipemia and disease treatment that peculiar effect is arranged to early stage uremic, allow clinically patient rely * * woman took the junzhisu bacteria liquid mixture Type B in 61 years old 10-20ml/ time, 4 times/day, blood urea nitrogen is reduced to 18mg% by 33mg% around one course of treatment, creatinine is reduced to 1.9mg% by 3.2mg%, and graft function is normal; The interior total protein of body rises to 5.7g% by 5.0g% after two courses of treatment, and cholesterol is reduced to 282mg% by 370mg%, and heart rate is reduced to 70 times/minute by 106 times/minute, and the rhythm of the heart transfers to for 3 times/minute by stopping fighting normally, goes through six and does not have recurrence.For another example: junzhisu bacteria liquid mixture C type can be used for the treatment of and shows as more antagonistic diseases clinically.Hyperpietic's height * * man took junzhisu bacteria liquid mixture C type one month in 58 years old, and (10-20ml/ time, 3 times/day) systolic pressure is reduced to the 130mmHg diastolic pressure from 180mmHg and is reduced to 90mmHg from 100mmHg; Lu hypotensive * * male 33 years old, took junzhisu bacteria liquid mixture C type one month, be upgraded to the 120mmHg diastolic pressure from 80mmHg and be upgraded to 80mmHg from 60mmHGg.
2, long-lasting other similar medicine that also is better than of junzhisu bacteria liquid mixture drug effect, when for example junzhisu bacteria liquid mixture A type is in order to nervous system regulation, carsick (ship) patient there is specially good effect, the patient takes behind 10 1 20ml and can take effect in 10 minutes, effective percentage reaches 100%, and its effect can keep 10-24 hours, and the patient feels freely.Kindred circumstances allows the patient take the carsick clever sheet of Western medicine, and effectively the patient only can keep 4 hours effectively, and the patient feels the lethargic state of having dizzy spells.
3, junzhisu bacteria liquid mixture is to cultivate medicinal fungi with medicinal plants, and logical biological zymolysis method is extracted its quick-acting active ingredient, free type amino acid content has reached more than 17 kinds, and also contain other nutrition-concerted element (as vitamin, trace element etc.) of needed by human so can well coordinate human body fluid, control abnormity cell growth normal cell is grown up, and has no side effect again.Hysteroma and mastadenoma follow endocrine not normal, the patient for example Liu * * woman 36 years old is through taking the junzhisu bacteria liquid mixture Type B 10-15ml/ time, 3 times/day three weeks an of course of treatment, the state of an illness is promptly seen and is clearly better, after taking two courses of treatment, the tumor cell atresia, eumenorrhea and weight increase 3kg go through and did not have recurrence in 6 years.
4, junzhisu bacteria liquid mixture belongs to a kind of biological product of broad spectrum activity curative effect, contain activating components such as the various nutrients of needed by human body and coherin, there is broad spectrum activity to escort effect and continuity cellular machine energy effect to function of human body, can demonstrate rapid healing effect to the integrated patient of multiple difficult disease especially, and it is, obvious further to curative effect along with dosage increases.
5, junzhisu bacteria liquid mixture prepares the crude drug that raw material is got, and it is extensively wide to originate, and preparation technology's week is short, and equipment is comparatively simple, the extraction ratio height, and cost is relatively low honest and clean, and the ratio of performance to price is good.
Below by specific embodiment explanation the present invention,, can prepare different junzhisu bacteria liquid mixture series of products but can not limit the present invention in order to prove inventive concept exploitativeness of the present invention.
Embodiment 1, by junzhisu bacteria liquid mixture A type join by unitary type medicinal liquid Flammulina velutipes liquid, Poria liquid each 1.5%, lucidum liquid, Lentinus Edodes liquid, Auricularia liquid, pick up the ears liquid each 5%, and the mixed liquor 50% of above-mentioned mushroom.Select medicinal fungi for use: Flammulina velutipes, Poria, Lentinus Edodes, Ganoderma, Auricularia, each bacterial strain of picking up the ears, by the above-mentioned processing step that provides respectively through bacterial strain mutation, bacterial strain acclimatization culture, bacterial strain production cultivation, zymolysis extraction, filtration sterilization, make unitary type medicinal fungus liquid and mixed type medicinal fungus liquid, be made into junzhisu bacteria liquid mixture A type by the junzhisu bacteria liquid mixture formula proportion again.Bacterial strain mutagenic and breeding condition is among the embodiment 1: above-mentioned bacterium is planted to be inoculated in and cultivates after 3 days in the mid-30 ℃ of environment of PDA culture medium, put again and use the 30W quartz burner in the mutation case, cultivated 6 days according to 10 minutes rearmounted 33 ℃ of darkrooms respectively apart from 10cm, carrying out medicinal fungus, to plant acclimatization culture be with Semen phaseoli radiati 35% with the culture medium of produce cultivating, Semen Tritici aestivi 30%, Fructus Cucurbitae moschatae 12%, Herba Spinaciae, Semen sojae atricolor, Herba Imperatae each 5%, Folium Camelliae sinensis, Herb Gynostemmae Pentaphylli each 2.5%, pollen, enuleation date, Radix Morindae Officinalis each 1%, after handling by preparation technology, add the magnetic water of 6 times of amounts, after boiling 30 minutes, get filtrate and take advantage of a favourable situation to add agar 0.18%, be mixed with the domestication culture medium, connect bacterium and be placed on cultivation in 33 ℃ of incubators, filter out the bacterial strain that is suitable for the high temperature fertility, use for producing to go up.The culture medium of carrying out medicinal bacterial strain production cultivation is with Semen phaseoli radiati 35%, Semen Tritici aestivi 30%, Fructus Cucurbitae moschatae 12%, Herba Spinaciae, Semen sojae atricolor, Herba Imperatae each 5%, Folium Camelliae sinensis, Herb Gynostemmae Pentaphylli each 2.5%, pollen, enuleation date, Radix Morindae Officinalis each 1%, after preparation technology's processing, add magnetic water to water content 65%, and after regulating pH value 6 with Fructus Citri Limoniae, other adds behind 1.5% Gypsum Fibrosum, sucrose 1% mix homogeneously.Culture medium was put the 1.5rg/cm pressure sterilization 45 minutes, connect bacterium after the cooling, put in 25 ℃ of temperature and relative air humidity 80% environment and cultivated, and when making mycelia secrete a little pigment, at once temperature was improved 42 ℃ to stop thalli growth.In biological zymolysis extraction process, biology helps depressant prescription to prepare with following prescription: new fresh hen egg (shelling) 1.8%, fresh Mel 3%, vinegar 2.5%, vitamin C 0.183%, zinc gluconate 0.07%, magnetic water 92%, citric acid is regulated pH value to 5.5, and the batching uniform mixing forms.Carrying out biological zymolysis extraction process is to carry out when thalline stops cultivating 2 hours, and the unitary type medicinal liquid is when zymolysis is for the first time extracted, the 250ml/ bottle adds biological synergistic agent 200ml, and the 250ml/ bottle is extracted in zymolysis for the second time, adds the biological synergistic agent of 100ml, 45 ℃ of zymolysis temperature, 3.5 hours time.It is 0.5 times of compost amount that the mixed type medicinal liquid adds biological synergistic agent amount, 50 ℃ of zymolysis temperature, and PG value 4, zymolysis can be filtered in 2 hours.All the other preparation technologies such as above-mentioned carrying out.Junzhisu bacteria liquid mixture A type by 1 preparation of this example detects through Institute of Analysis of the Chinese Academy of Agricultural Sciences: this liquid medicinal liquid contains free amino acid and reaches 17 kinds more than, total amino acids 32.0466m/ml total sugar 6.7%, reducing sugar 3.19%, vitamin A IU trace, vitamin B1 .256mg/ml100g, VB0.095mg/100g, compositions such as VC174mg/100g and polysaccharide material and trace element.Show according to the related hospitals clinical practice: junzhisu bacteria liquid mixture A type to nervous system regulation as carsick, seasick, neurasthenia such as insomnia, vertebra is increased pain, eyes, ear are swollen and ache, and the nerve skin symptoms such as disease and sudden senile silly fruit disease of itching all there is remarkable result.
Embodiment 2, press junzhisu bacteria liquid mixture Type B prescription, by unitary type medicinal liquid Flammulina velutipes liquid 15%, Hericium erinaceus (Bull. Ex Fr.) Pers. liquid 10%, Volvariella volvacea (Bull.Ex Franch.) Singer liquid, lucidum liquid, the liquid of picking up the ears, Auricularia polytricha (Mout) Sacc. liquid, tremella liquid each 5% and form with the mixed liquor 50% of mushroom, junzhisu bacteria liquid mixture Type B preparation technology is with embodiment 1.Detecting station, Foochow according to national food industry output and quality detects, this medicinal liquid contains free type aminoacid and reaches 17 kinds of (except that cystine) total amino acids 16.91mg/g, total sugar 0.97%, reducing sugar 0.88%, amino nitrogen 0.33%100ml, crude protein 3.15g/100ml VB2.7mg/100g, VE0.17mg/100g, VC11.0mg/100g, iodine 27mg/kg, chromium 1.11mg/kg, and composition such as polysaccharide.Show through the related hospitals clinical practice: this medicinal liquid has short control effect to human body cell, promptly can control sick cell, and the opposing function has coordination cell balance growth effects such as the differential growth of promotion effect to normal cell.Animal tumor test is being shown sarcoma S180, S37, cervical cancer V14 are all had significantly and extremely significant anticancer tumor effect, tumour inhibiting rate is more than 40%, and along with dosage increases, press down the also corresponding raising of tumor pain, cancerous cell control is eliminated simultaneously, weight increase clinically, this medicinal liquid is to rodent ulcer, have remarkable atrophy curative effect to hysteroma, mastadenoma, and weight in patients all has increase, in addition, this medicinal liquid follows hyperlipemia and heart disease to follow anemia that peculiar effect is all arranged to child, juvenile body weight growth in stature to chronic atrophic gastritis and early stage uremic.
Embodiment 3, junzhisu bacteria liquid mixture C type is filled a prescription by the unitary type medicinal liquid: Flammulina velutipes liquid 10%, Volvariella volvacea (Bull.Ex Franch.) Singer liquid 10%, Hericium erinaceus (Bull. Ex Fr.) Pers. liquid, tremella liquid, tremella liquid, mushroom liquid, Poria liquid, Ganoderma liquid each 5% and the mixed type medicinal liquid 50% of above mushroom formulated, it prepares technology such as embodiment 1.Detecting station, Foochow according to national food industry product quality detects: junzhisu bacteria liquid mixture C type contains free state aminoacid 17 kinds (except that cystine), total amino acid content 31,5mg/g, total sugar 2.72%, reducing sugar 2.66%, full nitrogen 7.01g/100ml, amino nitrogen 0.4g/100g, VB2.51mg/100g, VB3.61/100g, V0.45mg/100g, iodine 9mg/kg, copper 2.0mg/kg, calcium 0.6mg/100g, ferrum 1.2mg/100g, zinc 6.9mg/100g, chromium 2.1mg/100g.Junzhisu bacteria liquid mixture C type has special effect to endocrine disorder, shows according to the related hospitals clinical practice, and this medicinal liquid is to impotence, hyposexuality, and andropathys such as cacospermia follow heart disease to diabetes, and symptoms such as the depleted disease of mental and physical efforts II are all had original effect rapidly.
As can be seen from the above embodiments, junzhisu bacteria liquid mixture series of the present invention is to utilize the physiologically active medicine of natural pharmaceutical resources preparation, activating components such as the various essential nutrient of content needed by human body and coherin, therefore junzhisu bacteria liquid mixture has broad spectrum activity to escort effect and continuity cellular machine energy effect to function of human body, can demonstrate unique speed to the integrated patient of multiple difficult disease especially and treat and cure effect, this is Chinese medicine, crude drug, the irreplaceable effect of Western medicine so far.