CN105998061A - Bacterium medicine for treating cancer and preparation method - Google Patents

Bacterium medicine for treating cancer and preparation method Download PDF

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CN105998061A
CN105998061A CN201610455193.9A CN201610455193A CN105998061A CN 105998061 A CN105998061 A CN 105998061A CN 201610455193 A CN201610455193 A CN 201610455193A CN 105998061 A CN105998061 A CN 105998061A
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preparation
treating cancer
bacterium medicine
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bacterium
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钱师良
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    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
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Abstract

The invention discloses a bacterium medicine for treating cancer and a preparation method. The bacterium medicine is prepared from 1.2 mg/ml to 1.6 mg/ml of free amino acid, 5.3 mg/ml to 5.8 mg/ml of hydrolytic amino acid, 0.23 mg/ml to 0.27 mg/ml of nucleotide, 0.15 mg/ml to 0.18 mg/ml of RNA, 0.10 mg/ml to 0.12 mg/ml of DNA, 7.0 mg/ml to 7.4 mg/ml of folic acid, 95.5 mg/ml to 99.5 mg/ml of pantothenic acid, 1.8 mg/ml to 2.1 mg/ml of polysaccharide, 3.3 mg/ml to 3.6 mg/ml of vitamin B6, 30.4 mg/ml to 33.4 mg/ml of vitamin B12 and 0.13 mg/ml to 0.19 mg/ml of copper. The bacterium medicine can prevent the cancer, has a remarkable effect on the aspects of relieving various kinds of pain of a patient, improving the life quality of the cancerous patient, increasing the cure rate of the cancerous patient, prolonging the life of the cancerous patient and the like and is especially suitable for being used by the terminal cancer patient who does not want to accept radiotherapy and chemotherapy in the hospital and cannot be subjected to radiotherapy and chemotherapy due to the too large age or poor health.

Description

A kind of bacterium medicine treating cancer and preparation method
Technical field
The present invention relates to medicine field, in particular to a kind of bacterium medicine treating cancer and preparation method.
Background technology
In recent years, pathogenesis of cancer number and death toll are constantly increasing, " Chinese tumor registration annual report in 2012 " display, the annual new cancer cases in the whole nation has 3,120,000, that is, whole nation average minute clock has 6 people to be diagnosed as cancer, has 5 people to die from cancer, and the annual whole nation reaches ten thousand people more than 250 because of the case of cancer mortality.World Health Organization (WHO) predicts, to the year two thousand twenty, annual new cancer disease number will be more than 15,000,000 people, and cancer has become as first killer of the new century mankind, and becomes the public health problem that the whole world is maximum.
At present, the Therapeutic Method that cancer is traditional mainly has " treatment by Chinese herbs, operative treatment, radiotherapy, chemotherapy " four kinds of methods.Treatment by Chinese herbs belongs to tcm therapy, and operative treatment, radiotherapy, chemotherapy belong to western medicine therapy.
In terms of oncotherapy, Chinese and western medicine is respectively arranged with respective advantage and defect.The advantage of western medical treatment is instant effect, but side effect is too much, often causes the adverse consequences that things turn out contrary to one's wishes because of over-treatment, and side effect severe patient can cause single system or multisystem nonfunction, life threatening, causing many patient treatment final daies is exactly day of death.Advantages of TCM is that infringement iatrogenic to the secondary in the middle of cancer Radiotherapy chemotherapy has good auxiliary therapeutic action, it is possible to treat for cause of disease, but take effect slowly, curative effect the most notable.The means using the combination of Chinese and Western medicine can make both have complementary advantages, can reach the purpose improving comprehensive therapeutic effect, but current therapeutic method of traditional Chinese and western medicine all exists that treatment easily causes systemic-function exhaustion, misery index is high, cure rate is low, the unsatisfactory part of the high many of expense, the treatment of cancer is in the urgent need to starting new treatment of cancer theory, Therapeutic Method, medicine.
Modern diseases is changing disease disease spectrum to the threat of the mankind, require medical model by simple disease treatment to the Mode change preventing, keeping healthy, treat, rehabilitation combines, various alternative medicines and traditional medicine will be played a greater and greater role, and the world trends of " back to nature " that formed under this background are just promoting international community to natural drug and the pay attention to day by day of nutrient drug and growth.Integration of edible and medicinal herbs, doctor's (treatment) food (treatment) are integrally, modern medicine development and the trend of clinical treatment and the optimised form of medical treatment are become, therefore, develop, produce a kind of in replacing, the doctor trained in Western medicine treatment means to cancer and the product of medicine: there is abundant nutrition, delicious flavour and to have the edible fungi medicine of high health value and therapeutic effect be the direction that mankind itself develops effort.
Summary of the invention
For the problem also not having the most spendable a kind of bacterium medicine treating cancer in prior art, the present invention is proposed, to this end, present invention is primarily targeted at a kind of bacterium medicine treating cancer of offer and preparation method, to solve the problems referred to above.
The present invention solve above-mentioned technical problem use techniques below scheme:
nullA kind of bacterium medicine treating cancer and preparation method，It is characterized in that: free amino acid total amount 1.2mg/ml to 1.6mg/ml、Hydrolysis amino acid total amount 5.3mg/ml to 5.8mg/ml、Nucleotide 0.23mg/ml to 0.27mg/ml、RNA 0.15mg/ml to 0.18mg/ml、DNA 0.10mg/ml to 0.12mg/ml、Folic acid 7.0 μ g/ml to 7.4 μ g/ml、Pantothenic acid 95.5 μ g/ml to 99.5 μ g/ml、Polysaccharide 1.8mg/ml to 2.1mg/ml、Vitamin B6 3.3 μ g/ml to 3.6 μ g/ml、Vitamin B12 30.4 μ g/ml to 33.4 μ g/ml、Copper 0.13mg/L to 0.19mg/L、Zinc 0.86mg/L to 0.91mg/L、Germanium 0.05 μ g/ml to 0.1 μ g/ml、Selenium 0.05 μ g/ml to 0.1 μ g/ml、Chromium 0.045 μ g/ml to 0.049 μ g/ml、Manganese 0.54mg/L to 0.59mg/L、Vitamin C 19.6 μ g/ml to 20.1 μ g/ml、Adenine 0.43 μ g/ml to 0.5 μ g/ml.
Preferably, described a kind of its above-mentioned all of component content of bacterium medicine treating cancer is all the contained natural nutrient component of mycelium, sporophore or spore by wild or tame edible fungi self and ingredient combines, must not prepare by the artificial method adding these chemical compositions, lose natural drug and the nutrient drug theory of patent of the present invention " back to nature " the most completely.
Preferably, described free amino acid total amount is 1.6mg/ml.
Preferably, described hydrolysis amino acid total amount is 5.8mg/ml.
Preferably, described folic acid is 7.4 μ g/ml.
Preferably, described polysaccharide is 2.1mg/ml.
Preferably, described germanium is 0.1 μ g/ml.
Preferably, described selenium is 0.1 μ g/ml.
Preferably, the raw material of a kind of bacterium medicine treating cancer can be mycelium manufacture, simple sporophore manufacture or the simple spore manufacture that edible fungi is simple, it is also possible to be that edible fungi mycelium, sporophore or spore three mix manufacture according to a certain percentage.But, the method of the mycelium manufacture that employing edible fungi is simple is the first-selected best approach of a kind of bacterium medicine treating cancer, also it is the invention emphasis of this patent, the method using simple mycelium manufacture can be settled a dispute by the parties concerned themselves the difficulty that raw materials for production are unable to supply completely, can scientifically control the good and bad problem in raw materials for production, the quality making product is guaranteed, and the large-scale group type of particularly suitable batch production produces, it is ensured that the supply of product, popularization and clinical practice.
The invention has the beneficial effects as follows:
A kind of bacterium medical instrument treating cancer that the method is prepared has significantly treatment cancer effect, it can not only the generation of prophylaxis of cancer and also alleviating the various miseries of cancer patient, improving the life quality of cancer patient and improve cancer patient's cure rate, extending the aspects such as cancer patient's life has remarkable result.Particularly suitable those be unwilling hospital carry out Radiotherapy chemotherapy and because older or physical weakness and cannot hospital carry out Radiotherapy chemotherapy treatment patient with advanced cancer use.
Detailed description of the invention
A kind of bacterium medicine being specifically designed to treatment of cancer and the preparation method of the present invention are further described by embodiment.
It should be noted that in the case of not conflicting, the embodiment in the application and the feature in embodiment can be mutually combined.
1, mother culture media employing formula: horse official seal potato 30%, sucrose 3%, agar 2%, water 100%.
Pedigree seed culture medium employing formula: Semen Maydis powder 2%, Testa oryzae 2%, Testa Tritici 2%, sucrose 3%, water 100%.
Fermentation tank culture medium employing formula: Semen Maydis powder 1%, Testa oryzae 3%, Testa Tritici 3%, analysis for soybean powder 1%, sucrose 1%, water 100%.
2, Mother culture matrix manufacturing: culture medium boils 20 30 minutes, after extracting liquid, loading in 18mm × 200mm test tube, every test tube charge weight is 25 30%, tampon beyond the Great Wall, it is placed in sterilizing (1,000 grams/cm) 20 30 minutes in pressure cooker, sterilizing is put into slant medium after taking the dish out of the pot, and aseptically inoculates after cooling, and inoculum concentration is 8 10%, spawn culture can use for 7 to 10 days, and cultivation temperature is 25 DEG C.
Primary spawn matrix manufacturing: culture medium boils 10 15 minutes, after extracting liquid, load in 750ml glass jar, every bottle of charge weight is 150 200ml, being placed in sterilizing (1,000 grams/cm) 30 minutes in pressure cooker, sterilizing takes the dish out of the pot and aseptically inoculates after cooling, and sowing quantity is 15 20% (every test tube stock inoculates 56 bottles), spawn culture can use for 25 to 28 days, and cultivation temperature is 25 DEG C.
Fermentor cultivation matrix manufacturing: culture medium boils 10 15 minutes, after extracting liquid, load in fermentation tank, the constant volume of culture fluid is the 70% of tank body total capacity, first with (2,500,000 grams/cm) sterilizing 60 minutes before inoculation, then reach again complete sterilizing with 115 118 DEG C of continuous sterilizations and be as the criterion to not destroying culture medium nutrient.After sterilization, i.e. can inoculate when tank body is cooled to 25 DEG C, sowing quantity is 10%.Cultivation temperature early stage is 25 26 DEG C, and the later stage is 26 27 DEG C, and the fermentation tank of 500 liters can produce 3 ton of half culture fluid for 3 days.
3, when after the mycelia culture liquid maturation cultivated in fermentation tank, aseptically, loading in sterilization 750ml glass jar thoroughly, every bottle of charge weight is 200ml, beyond the Great Wall with the bottle stopper of purification passage, insert in the Multilayer culture shelf in clean training workshop and cultivate.Culture period is 125 130 days, and in clean training workshop, cultivation temperature is 25 26 DEG C, and humidity is 60 65.Culture fluid in each culture bottle is shaken about 30 seconds every 10 days at the first month cultivated；The second month cultivated to shake the culture fluid in each culture bottle about 45 seconds every 8 days；Three month cultivated to culture period terminates the culture fluid in each culture bottle was shaken about 1 minute every 6 days.
4, the mycelium of each kind that cultivation has grown in pouring out culture bottle respectively after terminating, prepare in the ratio in a kind of bacterium medicine composition treating cancer, inserting after the mycelium mixing prepared, reactor boils 30 minutes, filter with centrifuge (12 layers of gauze) again, using filling machine to load in 250ml vial B bottle, charge weight is 250 265ml.
5, canned mycelia mixture is placed in sterilizing (1,000 grams/cm) 30 minutes in pressure cooker, sterilization time opens autoclave door after terminating, in pot, cover the Cover inside the bottle of the most together sterilizing while hot, after the cooling that takes the dish out of the pot, aseptically cover the production technologies such as bottle enclosing cover envelope bottleneck, vanning (every case fills 24 bottles).
6, finished product early stage produces and terminates, in loading packing crates, rear entrance in incubator carries out the post-fermentation and culture stage, 10 13 floor heights chest being piled into 10 row × 10 row are a pile cultivation and fermentation heap, the first half of the year is every two weeks turning once, the second half year is every turning in 20 to 25 days once, during turning, require under the finished product box placement on top, outside middle finished product box placement, beneath finished product box is placed on top, the finished product box of outside is placed middle, listing supply can be started through 1 year post-fermentation and culture product and use in clinic.
7, a kind of bacterium medicine patent of invention product treating cancer all has therapeutical effect to the various cancers having now been found that.
Embodiment described above, the simply one of the present invention more preferably detailed description of the invention, the usual variations and alternatives that those skilled in the art is carried out in the range of technical solution of the present invention all should comprise within the scope of the present invention.

Claims (20)

1. the bacterium medicine treating cancer and preparation method, it is characterised in that its content includes: Free amino acid total amount 1.2mg/ml to 1.6mg/ml, hydrolysis amino acid total amount 5.3mg/ml to 5.8mg/ml, nucleotide 0.23mg/ml to 0.27mg/ml, RNA 0.15mg/ml to 0.18mg/ml, DNA 0.10mg/ml to 0.12mg/ml, folic acid 7.0 μ g/ml to 7.4 μ g/ml, pantothenic acid 95.5 μ g/ml to 99.5 μ g/ml, polysaccharide 1.8mg/ml to 2.1mg/ml, vitamin B6 3.3 μ g/ml to 3.6 μ g/ml, dimension Raw element B12 30.4 μ g/ml to 33.4 μ g/ml, copper 0.13mg/L to 0.19mg/L, Zinc 0.86mg/L to 0.91mg/L, germanium 0.05 μ g/ml to 0.1 μ g/ml, selenium 0.05 μ g/ml to 0.1 μ g/ml, chromium 0.045 μ g/ml to 0.049 μ g/ml, manganese 0.54mg/L to 0.59mg/L, vitamin C 19.6 μ g/ml to 20.1 μ g/ml, Adenine 0.43 μ g/ml to 0.5 μ g/ml.
A kind of bacterium medicine treating cancer the most according to claim 1 and preparation method, it is special Levy and be: described a kind of treat cancer its above-mentioned all of component content of bacterium medicine be all Contained by mycelium, sporophore or the spore self of wild or tame edible fungi Some natural nutrient components and ingredient combine, and must not add this with artificial The method of a little chemical compositions is prepared.
A kind of bacterium medicine treating cancer the most according to claim 1 and preparation method, it is special Levying and be, described free amino acid total amount is 1.6mg/ml.
A kind of bacterium medicine treating cancer the most according to claim 1 and preparation method, it is special Levying and be, described hydrolysis amino acid total amount is 5.8mg/ml.
A kind of bacterium medicine treating cancer the most according to claim 1 and preparation method, it is special Levying and be, described folic acid is 7.4 μ g/ml.
A kind of bacterium medicine treating cancer the most according to claim 1 and preparation method, its feature Being, described polysaccharide is 2.1mg/ml.
A kind of bacterium medicine treating cancer the most according to claim 1 and preparation method, it is special Levying and be, described germanium is 0.1 μ g/ml.
A kind of bacterium medicine treating cancer the most according to claim 1 and preparation method, it is special Levying and be, described selenium is 0.1 μ g/ml.
9. according to a kind of bacterium medicine treating cancer described in claim 1 to 8 and preparation method, It is characterized in that: the raw material of a kind of bacterium medicine treating cancer can be that edible fungi is simple Mycelium manufacture, simple sporophore manufacture or simple spore manufacture, it is also possible to be Edible fungi mycelium, sporophore or spore three mix manufacture according to a certain percentage.But It is that the method for the mycelium manufacture that employing edible fungi is simple is a kind of bacterium treating cancer The first-selected best approach of medicine, is also the invention emphasis of this patent, uses simple mycelia The method of body manufacture can be settled a dispute by the parties concerned themselves the difficulty that raw materials for production are unable to supply completely, can Scientifically to control the good and bad problem in raw materials for production, the quality of product is made to be guaranteed, The large-scale group type of particularly suitable batch production produces, it is ensured that the supply of product, popularization And clinical practice.
10. according to a kind of bacterium medicine treating cancer described in claim 1 to 8 and preparation method, Its composition of raw materials is characterised by: purple leather ear 8 10%, Schizophyllum commune Franch． 8 10%, open country Mushroom 8 10%, ricetulus rodents 8 10%, reddish brown squama mushroom 8 10%, long and narrow spore Ganoderma 8 10%, Cordyceps 8 10%, Ganoderma 6 10%, Russula delica 5 7%, Lentinus Edodes 5 7%, long handle Lycoperdon pyriforme 5 7%, Agroeybe cylindracea mycelia 4 7%, Auricularia polytricha (Mout) Sacc. 4 7%.
11. according to a kind of bacterium medicine treating cancer described in claim 10 and preparation method, its Composition of raw materials is characterised by: described Schizophyllum commune Franch． is 10%.
12. according to a kind of bacterium medicine treating cancer described in claim 10 and preparation method, its Composition of raw materials is characterised by: described ricetulus rodents is 10%.
13. according to a kind of bacterium medicine treating cancer described in claim 10 and preparation method, its Composition of raw materials is characterised by: described long and narrow spore Ganoderma is 10%.
14. according to a kind of bacterium medicine treating cancer described in claim 10 and preparation method, its Composition of raw materials is characterised by: described Cordyceps is 10%.
15. according to a kind of bacterium medicine treating cancer described in claim 10 and preparation method, its Composition of raw materials is characterised by: described Auricularia polytricha (Mout) Sacc. 7%.
16. according to a kind of bacterium medicine treating cancer described in claim 9 and preparation method, its system Preparation Method is characterised by, the side of the Mycelium culture manufacture that described employing edible fungi is simple Method.
17. according to a kind of bacterium medicine treating cancer described in claim 16 and preparation method, its Preparation method characteristic is, described ferment in second time production technology is of patent of the present invention The most crucial technology, can make the bacterium of this patent product by ferment in second time production technology Silk amount reproduction, increases considerably mycelium production, makes intramycelial cancer-resisting substance become Get growth at double.
18. according to a kind of bacterium medicine treating cancer described in claim 16 and preparation method, its Preparation method characteristic is, described in the mycelium boiling time in a kettle. for preparing be 30 minutes；250ml vial B bottle, charge weight is 257 258ml.
19. according to a kind of bacterium medicine treating cancer described in claim 16 and preparation method, its Preparation method characteristic is, the finished product chest in described incubator is piled into 10 row × 10 Row × 13 floor heights are a cultivation and fermentation heap.
20. according to a kind of bacterium medicine treating cancer described in claim 16 and preparation method, its Preparation method characteristic is, described post-fermentation and culture production technology be patent of the present invention again One key technology, after a kind of bacterium medicine finished product treating cancer is produced, its battalion Form point and ingredient also cannot be played, need to be combined into through post-fermentation and culture The multistage anticancer component of combined type, the time of post-fermentation and culture is the longest, the anticancer one-tenth of product Dividing the abundantest, mouthfeel is the best, the most notable to the treatment curative effect of cancer patient.Produce A kind of bacterium medicine finished product treating cancer come treatment to cancer patient in three months is effective Rate is 20%, and within three months to half a year, the treated effect to cancer patient is 30 35%, Within six months to nine months, the treated effect to cancer patient is 40 55%, nine months It is 60 80% to 12 months treated effects to cancer patient, within more than 1 year, is More than 80%.
CN201610455193.9A 2016-06-21 2016-06-21 Bacterium medicine for treating cancer and preparation method Pending CN105998061A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106722867A (en) * 2016-11-25 2017-05-31 钱仙麟 The mixed liquor that a kind of full nutrient formulation of tumour is made
CN109045072A (en) * 2018-04-01 2018-12-21 钱仙麟 A kind of edible mushroom liquid bacterium medicine for advanced cancer analgesic

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Publication number Priority date Publication date Assignee Title
CN1122715A (en) * 1994-11-10 1996-05-22 天津市农业生物工程研究中心 Functional oral liquor for enhancing human immunity
CN1124160A (en) * 1994-12-08 1996-06-12 卢宪 Preparing method for physiologically active medicine-junzhisu bacteria liquid mixture
CN1176756A (en) * 1997-09-13 1998-03-25 解玉岗 Nutrient and health-care liquid produced from dible fungi extract
CN1303622A (en) * 2000-05-12 2001-07-18 钱师良 Wuhong health-care beverage
CN1810951A (en) * 2004-11-19 2006-08-02 丁友昉 Fermented aweto and wolfberry fruit wine and its making process

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Publication number Priority date Publication date Assignee Title
CN1122715A (en) * 1994-11-10 1996-05-22 天津市农业生物工程研究中心 Functional oral liquor for enhancing human immunity
CN1124160A (en) * 1994-12-08 1996-06-12 卢宪 Preparing method for physiologically active medicine-junzhisu bacteria liquid mixture
CN1176756A (en) * 1997-09-13 1998-03-25 解玉岗 Nutrient and health-care liquid produced from dible fungi extract
CN1303622A (en) * 2000-05-12 2001-07-18 钱师良 Wuhong health-care beverage
CN1810951A (en) * 2004-11-19 2006-08-02 丁友昉 Fermented aweto and wolfberry fruit wine and its making process

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106722867A (en) * 2016-11-25 2017-05-31 钱仙麟 The mixed liquor that a kind of full nutrient formulation of tumour is made
CN109045072A (en) * 2018-04-01 2018-12-21 钱仙麟 A kind of edible mushroom liquid bacterium medicine for advanced cancer analgesic

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