CN105998373A - Health-caring oral liquid for treating hepatitis and preparation method thereof - Google Patents

Abstract

The invention relates to health-caring oral liquid for treating hepatitis and a preparation method thereof. The health-caring oral liquid comprises the following components by weight part: 5-8 parts of antrodia camphorate, 5-8 parts of Chinese magnoliavine, 5-8 parts of wolfberry, 5-8 parts of lucid ganoderma, 20-30 parts of a morchella broth, 20-30 parts of a golden mushroom broth, 20-30 parts of a grifola frondosus broth, and 10-12 parts of phellinus igniarius. The preparation method mainly comprises steps of extraction of the traditional Chinese medicines and wall breaking of mycelium. The preparation method is simple, maximum efficacy of the components can be performed, the health-caring oral liquid is benefit for absorption, and the raw materials cannot be wasted.

Description

A kind of health care oral liquid treating hepatitis and preparation method thereof

Technical field

The present invention relates to technical field of Chinese medicine, be specifically related to a kind of health care oral liquid treating hepatitis and preparation side thereof Method.

Background technology

Hepatitis is the general designation of inflammation.Typically refer to by multiple paathogenic factor--such as virus, antibacterial, parasite, chemistry Poisonous substance, medicine, ethanol, role of autoimmune factors etc. make liver cell be destroyed, and the function of liver suffers damage, and causes health one Series malaise symptoms, and the exception of liver function index.Spleen, kidney, mainly liver, can be involved in sick position, be characterized in the course of disease persistently, Touching endlessly, complicated symptoms, easily pass that it is dirty.

Without " hepatitis " name of disease in Ancient Chinese Medical Literature, but according to its clinical manifestation often occur hypochondriac pain, jaundice, Nausea and vomiting, Poor appetite, weak feature, can search the record of similar primary disease, belongs to the category of the diseases such as the traditional Chinese medical science " yellow cellulitis ", " hypochondriac pain ".

Burden of liver may be further resulted in by western medicine hepatitis at present to increase the weight of, and long-term taking side effect is serious.

Summary of the invention

For solving the problems referred to above, the present invention provides a kind of hepatoprotective effective, taking convenience, and can improve body immunity, The health care oral liquid of the treatment hepatitis that toxic and side effects is little.

Technical scheme:

A kind of health care oral liquid treating hepatitis, including the component of following weight portion: Antrodia Camphorata 5-8, Fructus Schisandrae Chinensis 5-8, Fructus Lycii 5- 8, Ganoderma 5-8, fermented morel liquid 20-30, Flammulina velutiper (Fr.) Sing fermentation liquid 20-30, Grifola gigantea (Pers.) Piat. Fermented liquid 20-30, Phellinus igniarius (L. ex Fr.) Quel. 10-12.

Further, including the component of following weight portion: Antrodia Camphorata 6, Fructus Schisandrae Chinensis 6, Fructus Lycii 6, Ganoderma 6, toadstool ferment Liquid 25, Flammulina velutiper (Fr.) Sing fermentation liquid 25, Grifola gigantea (Pers.) Piat. Fermented liquid 25, Phellinus igniarius (L. ex Fr.) Quel. 10.

Further, the preparation method of a kind of health care oral liquid treating hepatitis, it is characterised in that comprise the following steps:

(1) respectively by fermented morel liquid, Flammulina velutiper (Fr.) Sing fermentation liquid, Grifola gigantea (Pers.) Piat. Fermented liquid through 2500-3000 rev/min of centrifugal treating 10-15 minute, filter and obtain Morchella esculenta (L.) Pers mycelium, golden mushroom mycelium, maitake mushroom mycelia respectively, merge the preservation of above-mentioned filtrate and treat With；

(2) Morchella esculenta (L.) Pers mycelium that step (1) is filtrated to get respectively, golden mushroom mycelium, maitake mushroom mycelia carry out breaking cellular wall Process；

(3) Antrodia Camphorata, Fructus Schisandrae Chinensis, Fructus Lycii, Ganoderma, Phellinus igniarius (L. ex Fr.) Quel. are weighed according to above-mentioned weight portion, mixing, add mixture gross mass The water of 10-12 times, boils extraction 30-60min, filters, obtain filtrate；

(4) extracting solution of filtration step (3), filtering residue adds the water of step (3) mixture gross mass 2-4 times, boils extraction 30- 60min, filters, obtains filtrate；

(5) combining step (1), step (3) and the filtrate of step (4), dry in the air cool after add the Morchella esculenta (L.) Pers mycelium after described breaking cellular wall, Golden mushroom mycelium, maitake mushroom mycelia, mix.

The acquisition pattern of above-mentioned each fermentation liquid is the ordinary skill in the art, and those skilled in the art need not move through creation Property work can obtain, and experiment proves that obtaining the culture medium that used of each fermentation liquid has no significant effect the effect of the present invention, Also may select and separated from fermentation liquid by mycelium, redispersion replaces mycelium fermentation in the water of fermentation liquid equivalent Liquid, experiment proves that the two effect is suitable.Cell tissue broken wall treatment described in step (2) uses ultrasonic fragmentation, is also this The routine techniques in field.The culture medium that each fermentation liquid is used is: mass content is the glucose of 2%, and mass content is 9% bean Slag, mass content is the magnesium sulfate of 0.05%, and mass content is the potassium dihydrogen phosphate of 0.1%, and surplus is drinking water.Fermentation time is 6 days, fermentation temperature was 25 DEG C.

Antrodia Camphorata: be mainly derivant and some triterpenoid compound of Lanostane class, (ACE) can be suppressed to reach Purpose to blood pressure lowering.

Fructus Schisandrae Chinensis: astringe the lung, nourishing kidney, promote the production of body fluid, receive antiperspirant, arresting seminal emission.Controlling the deficiency of the lung to breath with cough, xerostomia is made thirsty, and spontaneous perspiration, night sweat, impairment caused by overstrain is thin Thin, emission, chronic diarrhea chronic dysentery.

Fructus Lycii: the effects such as sweet in the mouth, property are flat, have nourishing YIN and supplementing blood, replenishing vital essence to improve eyesight.The traditional Chinese medical science be usually used in treating because of the hepatic and renal YIN deficiency or Asthenia of essence and blood and the disease such as soreness of the waist and knees dizzy, dizzy, impotence and premature ejaculation, seminal emission, leukorrhagia and diabetes of causing.

Ganoderma: for strengthening body immunity, regulate blood glucose, controls blood pressure, adjuvant therapy chemicotherapy, hepatoprotective, promotees Enter the aspects such as sleep and be respectively provided with significant curative effect. there is the effect of building body.

Grifola frondosa: Grifola frondosa is nutritious, its nutrient content is through China Preventive Medicial Science Institute's nutrition and food hygiene Institute and the Ministry of Agriculture's each hectogram of Product Quality Verification Centers detection are done in Grifola frondosa and (are wherein contained needed by human body containing 25.2 grams of protein Amino acid/11 8 kinds 18.68 grams, wherein must base acid account for 45.5%) fat 3.2 grams, dietary fiber 33.7 grams, carbohydrate 21.4 Gram, ash 5.1 grams, mineral rich in multiple beneficial, potassium, phosphorus, ferrum, zinc, calcium, copper, selenium, chromium etc., vitamin content enriches, VE109.7 milligram, VB11.47 milligram, VB20.72 milligram, VC17.0 milligram, carotene 4.5 milligrams. various nutrients occupies each Planting first of edible fungi, wherein VB1 and VE content is the highest 10-20 times, and VC content is 3-5 times that it is similar, protein and aminoacid It is 2 times of Lentinus Edodes, can promote that children's torso is grown up healthy and sound and intelligent development, relevant arginine and lysine content relatively Flammulina velutiper (Fr.) Sing The content of middle lysine (1.024%) and arginine (1.231%) is high；The Aspartic Acid relevant with delicate flavour and content of glutamic acid are relatively Height, be therefore described as " edible fungi prince " with " North China Radix Ginseng ".Grifola frondosa has high medical health care function.According to document report Leading, it has suppression hypertension and effect of obesity；Due to rich in ferrum, copper and vitamin C, it can prevent anemia, vitamin C deficiency, white Purplish or white patches on the skin wind, prevents the generation of arteriosclerosis and cerebral thrombosis；Its selenium and chromium content are higher, protected liver, pancreas, prevention hepatitis and The effect of diabetes；Se content height makes it also have and prevents and treats Keshan disease, Kaschin-Beck disease and some cardiopathic function；It is held concurrently calcic And vitamin D, both coordinate, can effectively prevent and treat rickets；The favourable brain development of higher Zn content, holding oxyopia, Promote wound healing；The vitamin E of high-load and selenium coordinate, and make it defying age, memory reinforcing and sensitivity.Grifola frondosa Or noticeable anticarcinogen source, on the one hand, higher Se content has the effect resisting cancer, especially contained Grifola frondosa Polysaccharide (Grifolan), based on beta glucan (glucan), wherein active anticancer is the strongest, the β with 6 side chains-(1,3)-Portugal Polysaccharide accounts for sizable proportion, it is stated that than the lentinan (Lentinan, the PSK of Japan) appeared on the market, krestin (PSP in Shanghai) etc. have higher anti-cancer ability；It is again fabulous immunomodulator simultaneously.As Chinese medicine, Grifola frondosa and pig Siberian cocklebur equivalence, can control dysuria, edema, tinea pedis, hepatitis ascites and diabetes etc., be the most valuable medicinal fungi.

Morchella esculenta (L.) Pers: efficacy effect aspect, Morchella esculenta (L.) Pers has the kidney invigorating, tonifying YANG, supplementing the brain, the effect such as refresh oneself, the most nutritious, Delicious flavour, and benefit the intestines and stomach, be treatment dyspepsia, and the good Chinese medicine that abundant expectoration is breathed hard has the health-care effect of uniqueness.

Flammulina velutiper (Fr.) Sing: fresh Ion Absorption of Flammulina Velutipes is containing vitamin B group, vitamin C, carbohydrate, mineral, carotene, multiple Aminoacid, phytohaemagglutinin, polysaccharide, taurine, eritadenine, sterol Radix Ophiopogonis, cell dissolve toxin, Flammulina velutipes cytotoxin etc.. Tonifying liver, benefit the intestines and stomach, anticancer；Cure mainly the diseases such as hepatopathy, gastrointestinal tract inflammation, ulcer, carcinoma, Fructus Alpiniae Oxyphyllae.

Phellinus igniarius (L. ex Fr.) Quel.: antitumaous effect, liver protection effect anti-hepatic fibrosis, promote liver cell regeneration, can be used for preventing and treating chronic hepatitis, Hepatitis, hepatic ascites etc..Reduce and adjust blood sugar concentration, effectively prevent and improve diabetes.Reduce blood fat, prevent tremulous pulse hard Change, prevent the generation of cardiovascular and cerebrovascular disease.Prevention and treatment rheumatoid arthritis phellinus linteus extract can completely inhibit uric acid, right Gout has good result.Antiallergic, fine to allergic rhinitis, cureless eczema curative effect.

The usage and dosage of the present invention:

Within 1st, take twice, the most once, the most each 50 milliliters.

Beneficial effects of the present invention:

In inventive formulation, each component is respectively provided with hepatoprotective effect, the most also can interact after compounding, and produces Synergistic Effect, improves hepatoprotective effect further.The Chinese medicine of the present invention generally has effect of strong kidney enhancing human body immunity power, said components Common effect, can be effectively improved the antihypertensive effect of golden mushroom mycelium, maitake mushroom mycelia and Phellinus igniarius (L. ex Fr.) Quel..

Morchella esculenta (L.) Pers mycelium after breaking cellular wall has the kidney invigorating, tonifying YANG, supplementing the brain, the effect such as refresh oneself, the most nutritious, taste is fresh U.S., and benefit the intestines and stomach, be treatment dyspepsia, and the good Chinese medicine that abundant expectoration is breathed hard has the health-care effect of uniqueness.

Maitake mushroom mycelia after breaking cellular wall it have suppression hypertension and effect of obesity；Due to rich in ferrum, copper and Wei Ta Life C, it can prevent anemia, vitamin C deficiency, vitiligo, prevent the generation of arteriosclerosis and cerebral thrombosis；Its selenium and chromium content are higher, Protected liver, pancreas, prevention hepatitis and the effect of diabetes；Se content height makes it also have and prevents and treats Keshan disease, Kaschin-Beck disease With some cardiopathic function；It is held concurrently calcic and vitamin D, and both coordinate, and can effectively prevent and treat rickets；Higher Zn content Favourable brain development, holding oxyopia, promote wound healing；The vitamin E of high-load and selenium coordinate, and make it anti-ageing Always, memory reinforcing and sensitivity.

Golden mushroom mycelium tonifying liver after breaking cellular wall, benefit the intestines and stomach, anticancer；Cure mainly hepatopathy, gastrointestinal tract inflammation, ulcer, carcinoma etc. Disease, Fructus Alpiniae Oxyphyllae.

Additionally, the present invention uses mycelium, relatively sporophore cost significantly reduces, and mycelium is that filtering fermentation liquor obtains Mycelium, fermentation liquid is also added in oral liquid, does not waste the beneficiating ingredient in raw material, and the mycelium after breaking cellular wall is not compared to The mycelium of breaking cellular wall is easier to allow absorption of human body.Cultivating in gnotobasis, non agricultural chemical residuum, not by air pollution, it is ensured that drink The safe for drinking of material.

The preparation method of the present invention is simple, and can play the maximum drug effect of each composition, is beneficial to absorb, does not waste raw material.

Detailed description of the invention

Table 1 specific embodiment

Table 2 comparative example

Hepatitis B experiment case

Recovery from illness: the hepatitis B series three big positives are all turned out cloudy, clinical symptom disappearance, liver function and three big routines are normal.

Effective: hepatitis B series great three positive turns small three positive, clinical symptom relief more than 2/3, liver function and three big conventional improvements Reach more than 2/3.

Effective: hepatitis B series great three positive reverses more than 1/3, and liver function, three big routines take a favorable turn, and symptom has alleviated.

Invalid: unchanged before and after treatment.

ICR Strains of Mouse 70, male and female quantity is equal, body weight 20.1 ± 1.0g, rearing conditions: room temperature 25 ± 2 DEG C, manually Illumination 12h/d (h/d), by sex sub-cage rearing, 5, every cage, freely drinks water, and feeds with 21% high protein feed.Stochastic averagina divides Group, is divided into blank group, embodiment 1 group, embodiment 5 groups, embodiment 6 groups, comparative example 1 group, comparative example 2 groups, comparative example 3 Group, often group 10, the every day of each perfusion 2 milliliters sooner or later, blank group fills the drinking water of same dose.

Embodiment 1 group uses embodiment 1 oral liquid, and embodiment 5 groups uses embodiment 5 oral liquid, and embodiment 6 groups uses real Executing example 6 oral liquid, comparative example 1 group uses comparative example 1 oral liquid, and comparative example 2 groups uses comparative example 2 oral liquid, and comparative example 3 groups is adopted With comparative example 3 oral liquid, blank group fills the drinking water of same dose.30min after the mice last perfusion of each group, each Mus abdomen The chicken red blood cells suspension 0.25mL/10g of chamber injection 3%.

Chicken red blood cells suspension manufacturing methods: under aseptic condition, venous blood collection under the chicken wing, put in triangular flask, flask Middle elder generation is added with the Alsever of 5 times of blood flow volumes^,S liquid (sodium citrate .2H₂O 80g, Chinese holly edge acid 0.5, glucose 18.7g, sodium chloride 4.2g, adds distilled water and filters to 1000mL, in 68.9KPa pressure cooker after sterilizing 20min, and 4 DEG C of Refrigerator stores).Will be added with Alsever^,After the Sanguis Gallus domesticus of s liquid washs three times with 2000 turns/min of physiological saline solution, then it is made into the suspension of 3% with normal saline Liquid.Each group mouse peritoneal injected 3% chicken red blood cells suspension after 9 hours, and de-cervical vertebra is put to death, abdominal cut skin under aseptic condition Skin, intraperitoneal injection of saline 2 milliliters, gently rub abdominal part 2-3min, draw peritoneal irrigation and drip on microscope slide, every agreement that contracts a film or TV play to an actor or actress 0.2 milli Rising, incubation 30min in 37 DEG C of incubators, take out slide, normal saline rinses, and dries the fixing 5min of rear acetone-methanol (1:1), Dyeing 3-5min with 4%Giemsa-Wright liquid, distilled water rinses, oil Microscopic observation after natural drying again, and mice is huge bites for each group Cell phagocytic percentage and phagocytic index, t test statistics processes, the results are shown in Table 3.

Macrophage number/200 the macrophage * 100% of phagocytic percentage=wherein swallow chicken red blood cell

Chicken red blood cell sum/200 macrophages of phagocytic index=wherein swallowed

Embodiment 1 group, embodiment 5 groups, embodiment 6 groups, comparative example 1 group, comparative example 2 groups, comparative example 3 groups are made in accordance with the following methods Standby:

(1) respectively by fermented morel liquid, Flammulina velutiper (Fr.) Sing fermentation liquid, Grifola gigantea (Pers.) Piat. Fermented liquid through 3000 revs/min of centrifugal treating 15 points Clock, filters and obtains Morchella esculenta (L.) Pers mycelium, golden mushroom mycelium, maitake mushroom mycelia respectively, merges above-mentioned filtrate and preserves stand-by；

(2) Morchella esculenta (L.) Pers mycelium that step (1) is filtrated to get respectively, golden mushroom mycelium, maitake mushroom mycelia carry out breaking cellular wall Process；

(3) Antrodia Camphorata, Fructus Schisandrae Chinensis, Fructus Lycii, Ganoderma, Phellinus igniarius (L. ex Fr.) Quel. are weighed according to above-mentioned weight portion, mixing, add mixture gross mass The water of 10 times, boils extraction 45min, filters, obtain filtrate；

(4) extracting solution of filtration step (3), filtering residue adds the water of step (3) mixture gross mass 4 times, boils extraction 45min, filters, obtains filtrate；

(5) combining step (1), step (3) and the filtrate of step (4), dry in the air cool after add the Morchella esculenta (L.) Pers mycelium after described breaking cellular wall, Golden mushroom mycelium, maitake mushroom mycelia, mix.

The impact (X ± S) of table 3 Oral Liquid On Mice peritoneal macrophage phagocytosis chicken red blood cell ability

Result shows: compared with blank group, embodiment 1 group, embodiment 5 groups, embodiment 6 groups, comparative example 1 group, contrast Example 2 groups, the phagocytic percentage of 3 groups of Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cells of comparative example and phagocytic index value all increase, and say Bright embodiment 1, embodiment 5, embodiment 6, comparative example 1, comparative example 2, comparative example 3 oral liquid be respectively provided with raising mice huge bite thin Born of the same parents' phagocytic activity, increases body non-specific immunity effect.

Lanqin oral solutions

200 example Chronic Hepatitis B men and women half and half, the age, in 18-60 one full year of life, takes mouthful embodiment 6 oral liquid and checks after 3 months Every indices.

The research of table 4 clinical symptoms

Observation item	Pain in the hepatic region	Poor appetite	Feel sick	Regurgitation	Xerostomia	Bitter taste
							Before treatment	110	138	158	44	82	184
After treatment	0	2	0	0	0	0

Showing that oral liquid is all effective to above-mentioned symptom, effective percentage reaches 98.5%.

The result of study of table 5 hepatitis B Serial serum

200 example Chronic Hepatitis Bs, during taking oral liquid and after terminating the course for the treatment of, have no obvious toxic-side effects and untoward reaction Appearance, show oral liquid Clinical practice safety.

Described above to the disclosed embodiments, makes professional and technical personnel in the field be capable of or uses the present invention. Multiple amendment to these embodiments will be apparent from for those skilled in the art, as defined herein General Principle can realize without departing from the spirit or scope of the present invention in other embodiments.Therefore, the present invention It is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein and features of novelty phase one The widest scope caused.

Claims (3)

1. the health care oral liquid treating hepatitis, it is characterised in that include the component of following weight portion: Antrodia Camphorata 5-8, the five tastes Sub-5-8, Fructus Lycii 5-8, Ganoderma 5-8, fermented morel liquid 20-30, Flammulina velutiper (Fr.) Sing fermentation liquid 20-30, Grifola gigantea (Pers.) Piat. Fermented liquid 20-30, Phellinus igniarius (L. ex Fr.) Quel. 10-12.

A kind of health care oral liquid treating hepatitis the most according to claim 1, it is characterised in that include following weight portion Component: Antrodia Camphorata 6, Fructus Schisandrae Chinensis 6, Fructus Lycii 6, Ganoderma 6, fermented morel liquid 25, Flammulina velutiper (Fr.) Sing fermentation liquid 25, Grifola gigantea (Pers.) Piat. Fermented liquid 25, Phellinus igniarius (L. ex Fr.) Quel. 10.

3. the preparation method of a kind of health care oral liquid treating hepatitis described in claim 1 or 2, it is characterised in that include with Lower step:

(1) respectively by fermented morel liquid, Flammulina velutiper (Fr.) Sing fermentation liquid, Grifola gigantea (Pers.) Piat. Fermented liquid through 2500-3000 rev/min of centrifugal treating 10-15 minute, filter and obtain Morchella esculenta (L.) Pers mycelium, golden mushroom mycelium, maitake mushroom mycelia respectively, merge the preservation of above-mentioned filtrate and treat With；

(2) Morchella esculenta (L.) Pers mycelium that step (1) is filtrated to get respectively, golden mushroom mycelium, maitake mushroom mycelia carry out breaking cellular wall Process；

(3) Antrodia Camphorata, Fructus Schisandrae Chinensis, Fructus Lycii, Ganoderma, Phellinus igniarius (L. ex Fr.) Quel. are weighed according to above-mentioned weight portion, mixing, add mixture gross mass The water of 10-12 times, boils extraction 30-60min, filters, obtain filtrate；

(4) extracting solution of filtration step (3), filtering residue adds the water of step (3) mixture gross mass 2-4 times, boils extraction 30- 60min, filters, obtains filtrate；

(5) combining step (1), step (3) and the filtrate of step (4), dry in the air cool after add the Morchella esculenta (L.) Pers mycelium after described breaking cellular wall, Golden mushroom mycelium, maitake mushroom mycelia, mix.