CN102973725A

CN102973725A - Lentinan capsules

Info

Publication number: CN102973725A
Application number: CN 201210412332
Authority: CN
Inventors: 罗瑞雪
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-10-25
Filing date: 2012-10-25
Publication date: 2013-03-20

Abstract

The invention provides lentinan capsules. The lentinan capsules are manufactured by the following components of, by weight, 20-30 g of lentinula edodes, 10-20 g of fructus lycii, 5-10 g of ganoderma lucidum and 5-10 g of poria cocos. The lentinan capsules are prepared by the steps of weighing each component of the raw material drugs according to the weight proportion, grinding into fine powder respectively, decocting the lentinula edodes in a water bath at a temperature of 90-95 DEG C; screening with a 100-mesh screen; concentrating filtrate to a clear paste state; adding the fine powder of fructus lycii, ganoderma lucidum and poria cocos; mixing the powder uniformly, making the mixture into particles; drying the particles and loading the particles in capsules. The lentinan capsules have the effect of improving human immunity, and have homology of medicine and food, without any toxic and side effect.

Description

Lentinan

Technical field: the present invention relates to a kind of compound Chinese medicinal preparation that improves immunity.

Technical background:

Hepatic fibrosis be in the various chronic hepatopathys progress since in the liver fiber generate with degraded unbalancely, cause too much collagen due to intrahepatic deposition, often with inflammation and can develop into hepatitis interstitialis chronica.The genesis of hepatic fibrosis is relevant with immune status.Research in recent years shows: immunoregulation plays an important role in hepatic fibrosis, this mainly is because the liver blood supply is abundant, it is the center that body carries out mass exchange and metabolism, easily contact with external foreign body antigen, thereby active cell immune system and immunoreation occurs, and the further release cells factor, activate fat-storing cell and cause collagenic supersession disorderly, cause the generation of hepatic fibrosis.The raising body immunity can stop the generation of hepatic fibrosis.

Lentinan is the effective active composition that extracts from the quality xianggu sporophore, active component in the lentinan is β with branch-(1-3)-D-glucosan, main chain is comprised of the glucosyl group of β-(1-3)-connect, the glucosyl group that is connected by β-(1-6) along the main chain random distribution, be pectinate texture.Studies show that the active polysaccharide composition β of Lentinus Edodes-(1-3)-D-glucosan is to suppressing allos, homology or even genetic tumor is all effective.Lentinan scalable people Xiu Nei has the T cytoactive of immunologic function, can reduce the ability of methyl cholanthrene induced tumor.Chinese patent (94115213.8) discloses a kind of brain health oral liquor and manufacturing technology thereof, and its prescription is refine fish oil 10%, alginate jelly 1%, carboxymethyl cellulose 0.5%, soft plantation white sugar 20%, Saccharum Sinensis Roxb. 20%, the sucrose monoglyceride is an amount of, calcium chloride 4%, vitamin E 0.3%, citric acid is an amount of, lentinan 0.35%, and aquae destillata adds to 100%, its processing technology is water treatment, change glue, batching, pelletize, typing, rinsing, dewatering, lentinan is composite, canned, sterilization, the packing labeling, this invention has the moulding uniqueness and is suspended in the multiple liquid of lentinan for the refine fish oil micelle, features good taste, removed the fishy smell of other fish oil goods, be improved lipid composition, the health-care effect of free radical resisting oxidation and nourishing the brain and improving intelligence.Other has patent (201010129132.6) to disclose " a kind of product that improves skin metabolism and energy slow down aging and preparation method thereof ", belong to field of health care food, its active component comprises following component: Ganoderma extract, lentinan, Radix Puerariae isoflavone, soybean isoflavone, the weight portion of its component raw material is: 2～8 parts of 5～12 parts of Ganodermas, 1～5 part of lentinan, 1～8 part of Radix Puerariae isoflavone and soybean isoflavone.Experiment showed, that this invention herbaceous plant compositions can obviously improve skin metabolism, make it tender smooth, more flexible and gloss has played slow down aging, the effect of whitening and speckle dispelling.

Summary of the invention:

The purpose of this invention is to provide a kind of Lentinan, have the effect that improves immunity.

The present invention adopts Lentinus Edodes 20-30g, Fructus Lycii 10-20g, Ganoderma 5-10g, and the crude drug such as Poria 5-10g are made, and preparation method is, weight proportion by crude drug takes by weighing each component, and pulverization decocts Lentinus Edodes 90-95 ℃ of water-bath respectively, 100 orders filter, filtrate concentrates the clear paste shape, adds Fructus Lycii, Ganoderma, Poria fine powder, mixing, granulation, drying in incapsulating, and get final product.

Lentinan has immunological enhancement among the present invention, though its mechanism can be brought into play anti-tumor activity by the immunologic function of enhancing body in vivo without the effect of direct killing tumor cell.The NK cytoactive in spleen and abdominal cavity is strengthened, and inducement interferon is relevant with this product dosage, and its activity has synergism with interleukin class or interferon inducers.Other has proof, can strengthen the activity of the anti AIDS virus of deoxythymidine in external this product; The Fructus Lycii sweet in the mouth; Property is put down, and returns Liver Channel, kidney channel, lung meridian, has nourishing the liver; Nourishing kidney; The effect of lung moistening.Ganoderma is Chinese traditional valuable ingredient of Chinese medicine, possesses very high medical value, and the modern pharmacology of process scientific research institution many decades studies confirm that, Ganoderma is regulated blood glucose for strengthening body immunity, the control blood pressure, auxiliary tumor chemoradiotherapy, hepatoprotective, the aspects such as hypnotic all have significant curative effect; Poria sweet in the mouth, light, property are flat, be used as medicine have promoting diuresis to eliminate damp pathogen, the function of strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming.Modern medicine study: Poria energy enhancing human body immunity function, pachyman has obvious antitumor and protects the liver dirty effect.

Advantage of the present invention is:

Have the effect that improves immunity, and integration of edible and medicinal herbs, have no side effect.

Specific embodiments:

Embodiment 1: take by weighing respectively Lentinus Edodes 20g, Fructus Lycii 10g, Ganoderma 5g, the crude drug such as Poria 5g with various crude drug difference pulverizations, decoct Lentinus Edodes 90-95 ℃ of water-bath, 100 orders filter, filtrate concentrates the clear paste shape, adds Fructus Lycii, Ganoderma, Poria fine powder, mixing, granulation, drying in incapsulating, and get final product.

Embodiment 2: take by weighing respectively Lentinus Edodes 30g, Fructus Lycii 20g, Ganoderma 10g, the crude drug such as Poria 10g with various crude drug difference pulverizations, decoct Lentinus Edodes 90-95 ℃ of water-bath, 100 orders filter, filtrate concentrates the clear paste shape, adds Fructus Lycii, Ganoderma, Poria fine powder, mixing, granulation, drying in incapsulating, and get final product.

Embodiment 3: take by weighing respectively Lentinus Edodes 25g, Fructus Lycii 15g, Ganoderma 8g, the crude drug such as Poria 8g with various crude drug difference pulverizations, decoct Lentinus Edodes 90-95 ℃ of water-bath, 100 orders filter, filtrate concentrates the clear paste shape, adds Fructus Lycii, Ganoderma, Poria fine powder, mixing, granulation, drying in incapsulating, and get final product.

Embodiment 4: adopt mice reticuloendothelial system phagocytic functional experiment, the research Lentinan strengthens the effect of cellular immune function.

Adopt mice as animal subject, get 50 mices, male and female half and half, body weight are 18-22 g, are divided at random 5 groups: high dose group, middle dosage group, low dose group, and positive controls, model group, every group of l0 is only.Model group is according to the dosage gavage normal saline of 0.2ml/10g; Positive controls gavage 2.0g/kg lycium barbarum polysaccharide oral liquid; High, normal, basic dosage group gives respectively Lentinan 2.0g/kg, 1.0 g/kg, 0.5g/kg gavage, successive administration 20 days.30min gets and respectively organizes mice after the last administration, 25% india ink of tail vein injection 1% gelatin solution dilution, administration volume 0. 1ml/10g.30s and 6min mice vena orbitalis posterior clump are got blood 0. 025ml behind the injection prepared Chinese ink, be blown at once among the 0. 1%Na2CO3 liquid 2ml, suction pipe sucks in this liquid, blows out for several times, the blood that adheres to fully to wash out the suction pipe wall, it is complete to get blood, be dissolved in 2ml0. 1%Na2CO3 liquid school zero, 675nm place colorimetric on spectrophotometer with 0.025ml normal mouse blood.And after for the second time blood sampling, with taking off the cervical vertebra method mice is put to death, weigh, get liver, spleen is weighed, be calculated as follows phagocytic index k and engulf coefficient (correction phagocytic index) A:K=(lgOD1-lgOD2)/(T2-T1), A=W (K) ^1/3OD1, OD2 are 30s and 6min optical density in/the WLS formula, and T is the time, and W is body weight, and WLS is that the liver spleen closes heavily.

The result: such as table 1, the basic, normal, high dosage of Lentinan all can strengthen the mice reticuloendothelial system to the ability of cleaning up of engulfing of inertia carbon granule in the blood flow, and K value and A value are all seen obviously and increased (P＜0.05).

Table 1 Lentinan is on the impact of mice reticuloendothelial system phagocytic function (means ± s)

?	Medicine	Dosage	The animal number of elements	Phagocytic index k	Proofread and correct phagocytic index A
						Model group	Normal saline	0.2ml/10g	10	0. 0102±0.0011	3. 42 ± 0. 62
Positive group	Shiitake fungus polyoses oral liquid	2.0g/kg	10	0. 0311±0.0082*	6. 02 ± 1.23*
						High dose group	Lentinan	2.0g/kg	10	0. 0325±0.0074*	6. 24 ± 1.52*
Middle dosage group	Lentinan	1.0g/kg	10	0. 0295±0.0083*	5. 86 ± 0. 74*
						Low dose group	Lentinan	0.5g/kg	10	0. 0242±0.0086*	4.91 ± 0. 80*

* p＜0.05, * * p＜0.01 is compared with model group

Conclusion: Lentinan can strengthen mice reticuloendothelial system phagocytic function, promotes the effect of cellular immune function.

Embodiment 5: adopt the determination experiment of half hemolysis value (HC50), the research Lentinan strengthens the effect of mouse humoral immune function.

Adopt mice as animal subject, get 50 mices, male and female half and half, body weight are 18-22 g, are divided at random 5 groups: high dose group, middle dosage group, low dose group, and positive controls, model group, every group of l0 is only.Model group is according to the dosage gavage normal saline of 0.2ml/10g; Positive controls gavage 2.0g/kg shiitake fungus polyoses oral liquid; High, normal, basic dosage group gives respectively Lentinan 2.0g/kg, 1.0 g/kg, 0.5g/kg gavage, successive administration 20 days.The 8th day initial immunity of administration, sheep red blood cell (SRBC) (SRBC) 0.2mL of 5 groups of equal lumbar injections 5% of mice, administration again immunity in the 15th day, dosage is the same.The 20th day, after the last administration behind the 30min, mice vena orbitalis posterior clump is got blood 1ml, centrifugal, get serum, with the SA buffer serum is diluted 200 times, getting 1mL puts in vitro, add successively 10% (v/v is with the preparation of SA buffer) hematocrit SRBC 0.5 mL, complement 1mL (pressing the 1B10 dilution with the SA buffer).Other establishes the not control tube of increase serum (replacing with the SA buffer).After putting in 37 ℃ of waters bath with thermostatic control insulation 30min, the ice bath cessation reaction.The centrifugal 10min of 2000 rpm gets supernatant 1 mL, adds Dou Shi reagent 3 mL.(v/ v is with the preparation of SA buffer) hematocrit SRBC 0.25 mL that gets simultaneously 10%, add Dou Shi reagent to 4mL in another test tube, abundant mixing, place 10 min after, sentence control tube in 540nm and do blank, measure respectively and respectively manage light absorption value, calculate half hemolysis value HC50.The amount of hemolysin is calculated as follows with half hemolysis value (HC50) expression: the light absorption value * extension rate during half hemolysis value (HC50)=sample light absorption value/SRBC HD50.

Result: such as table 2.Compare with the model group mice, the basic, normal, high dosage of Lentinan makes antibody forming cell OD value increase (P＜0.05), serum hemolysin HC50 raise (P＜0.05).

Table 2 Lentinan is on the impact of HC50 (means ± s)

?	Medicine	Dosage	The animal number of elements	OD	HC50（g/Kg）
						Model group	Normal saline	0.2ml/10g	10	0. 425± 0. 032	109.52± 22.26
Positive group	Shiitake fungus polyoses oral liquid	2.0g/kg	10	0.570± 0. 121*	148.50± 35.01*
						High dose group	Lentinan	2.0g/kg	10	0. 624± 0. 132*	154.14± 41.13*
Middle dosage group	Lentinan	1.0g/kg	10	0. 583± 0. 119*	142.23± 40.23*
						Low dose group	Lentinan	0.5g/kg	10	0. 544± 0. 094*	134.35± 31.42*

* p＜0.05, * * p＜0.01 is compared with model group

Conclusion: Lentinan energy enhancing antibody forms cell OD value and serum hemolysin HC50 increases, and strengthens the effect of humoral immune function.

Claims

1. a Lentinan is characterized in that: be comprised of following crude drug: Lentinus Edodes 20-30g, Fructus Lycii 10-20g, Ganoderma 5-10g, Poria 5-10g.

2. according to claims 1 described Lentinan preparation method, it is characterized in that: the weight proportion by crude drug takes by weighing each component, and pulverization decocts Lentinus Edodes 90-95 ℃ of water-bath respectively, 100 orders filter, filtrate concentrates the clear paste shape, adds Fructus Lycii, Ganoderma, Poria fine powder, mixing, granulation, drying in incapsulating, and get final product.